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FuzzyID2: A software package for large dataset species identification via barcoding and metabarcoding using Hidden Markov Models and fuzzy set methods

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FuzzyID2

FuzzyID2: A software package for large dataset species identification via barcoding and metabarcoding using Hidden Markov Models and fuzzy set methods

This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version.

The following restriction to the GNU GPL applies: contact the author of this program (http://willpearse.github.com/phyloGenerator) for a suitable citation when publishing work that uses this code.

This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU General Public License for more details.

You should have received a copy of the GNU General Public License along with this program. If not, see http://www.gnu.org/licenses/.

FuzzyID2 installation: x86_64 compile command: g++ main.cpp mainFunction.cpp commonFunction.cpp -o FuzzyID2_x86_64 -lsqlite3 -lbpp-core -lbpp-seq -lbpp-phyl -Wl,-rpath ./lib64 -L ./lib64 -I ./include ln -s FuzzyID2_x86_64 FuzzyID2

i686 compile command: g++ main.cpp mainFunction.cpp commonFunction.cpp -o FuzzyID2_i686 -lsqlite3 -lbpp-core -lbpp-seq -lbpp-phyl -Wl,-rpath ./lib -L ./lib -I ./include ln -s FuzzyID2_i686 FuzzyID2

FuzzyID2 execute commands: (1)reference database construction python3 FuzzyID2_makeDB.py #parameters should be set in config file(config.txt) (2)sequence identification 1)single marker ./FuzzyID2 -c ID -in ./test/Noc_COI.fas -m K2P -out ./test/Noc-out.txt -d Noctuidae 2)metabarcode markers ./FuzzyID2 -c ID -in ./test/query_metabarcode.fas -m K2P -out ./test/Metabarcode_out.txt -d Metabarcode 3)Plant markers ./FuzzyID2 -c ID -in ./test/query_rbcl.fas+./test/query_trnl.fas -m K2P -out ./test/Plant_out.txt -d Plant -rb rbcl -mb trnl

#For more information, please read the FuzzyID2-guide.pdf.

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FuzzyID2: A software package for large dataset species identification via barcoding and metabarcoding using Hidden Markov Models and fuzzy set methods

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