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Update README.md
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yaaminiv authored Nov 17, 2016
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Expand Up @@ -15,7 +15,18 @@ This subdirectory hosts the Jupyter (iPython) notebooks that detail reproducible
- Displays FastQC results from `2016-10-13-oly-gonad-OA-part-1.ipynb`

[`2016-10-28-oly-gonad-OA-part-2-BLAST.ipynb`](https://github.com/yaaminiv/yaaminiv-fish546-2016/blob/master/notebooks/2016-10-28-oly-gonad-OA-part-2-BLAST.ipynb)
- Ran BLAST of draft *O. lurida* transcriptome against specialized Uniprot database (in progress as of 2016-11-04, to be completed by 2016-11-07).
- Ran BLAST of draft *O. lurida* transcriptome against specialized Uniprot database that includes gene ontology information
- Updated blastx arguments to rerun blastx and produce only best matches with Uniprot database

[`2016-11-04-oly-gonad-OA-kallisto.ipynb`](https://github.com/yaaminiv/yaaminiv-fish546-2016/blob/master/notebooks/2016-11-04-oly-gonad-OA-part3-kallisto.ipynb)
- Ran Kallisto to get count data (in progress as of 2016-11-04, to be completed by 2016-11-07).
- Ran Kallisto to get count data

[`2016-11-11-oly-gonad-OA-part4-differential-expression.ipynb`](https://github.com/yaaminiv/yaaminiv-fish546-2016/blob/master/notebooks/2016-11-11-oly-gonad-OA-part4-differential-expression.ipynb)
- Took count data and ran through DESeq2
- Identified genes differentially expressed at different levels of significance
- Compared the following sets:
- All .fastq files
- Female_106 vs. Female_108
- Male_106 vs. Male_108
- Female_106 vs. Male_106
- Female_108 vs. Male_108

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