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quality_trimmer.py is a command line application for quality trimming of fastq files. usage: python quality_trimmer.py infile x y z where: infile = input file to be trimmed. Must be fastq format (preferably with .fastq suffix) x = minimum average quality across sliding window, (Phred score), e.g. 57 y = minimum length after trimming to keep read, e.g. 30 z = window size, e.g. 10 example session (at the command line prompt): python quality_trimmer.py example.fastq 57 30 10 # note: failed sequencess are excluded from the final output
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