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I have performed a velocity analysis on data from two different conditions. TI have identified differential velocities by cluster already using the tl.rank_velocity_genes() function, but I would like to perform pairwise comparisons between velocity estimates for the two conditions within the same clusters. I have 7 clusters, so for example if I have inflamed_cluster0 through inflamed_cluster7 and control_cluster0 through control_cluster0 how can I compare inflamed_cluster0 vs control_cluster0? and inflamed_cluster7 vs control_cluster7?
Should I output the values from the velocity layer and do a t-test? Or do you have another suggestion for how best to perform this analysis?
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I have performed a velocity analysis on data from two different conditions. TI have identified differential velocities by cluster already using the tl.rank_velocity_genes() function, but I would like to perform pairwise comparisons between velocity estimates for the two conditions within the same clusters. I have 7 clusters, so for example if I have inflamed_cluster0 through inflamed_cluster7 and control_cluster0 through control_cluster0 how can I compare inflamed_cluster0 vs control_cluster0? and inflamed_cluster7 vs control_cluster7?
Should I output the values from the velocity layer and do a t-test? Or do you have another suggestion for how best to perform this analysis?
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