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I am currently working on a project where I am integrating 10x Genomics PolyA-enriched RNA sequencing data with Fixed RNA Profiling data (with bbknn) to quantify macrophage and monocyte populations. Given that the Fixed RNA Profiling is based on probes that are only 14% spliced, I have concerns regarding the impact this might have on the results of my analysis. #267
This discussion was converted from issue #1138 on December 01, 2023 14:34.
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I am currently working on a project where I am integrating 10x Genomics PolyA-enriched RNA sequencing data with Fixed RNA Profiling data (with bbknn) to quantify macrophage and monocyte populations. Given that the Fixed RNA Profiling is based on probes that are only 14% spliced, I have concerns regarding the impact this might have on the results of my analysis. #267
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