SpotAssayQuant

Spot Assay Quantification Pipeline for the O'Donnell Lab, University of Pittsburgh.

Video Tutorials

ImageJ Batch Cropping: https://vimeo.com/441141948/932277a750

ImageJ Batch Conversion to Binary: https://vimeo.com/441391587/b51fc8f232

CellProfiler Spot Assay Pipeline: https://vimeo.com/441173290/ab3a57f6d3

CellProfiler Edit Mode Commands: https://vimeo.com/441200392/43c79497c6

Spot Assay Pipeline Extra: https://vimeo.com/441461749/e323b0393d

Instructions

1. Download CellProfiler: https://cellprofiler.org/

2. Follow the Tutorial to set up and run the pipeline.

3. Run result directory through CPGraphSort. See Tutorial for more details.