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Bad dataset analysis
Ryan Wick edited this page Jul 7, 2020
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To begin, I ran Trycycler cluster on the assemblies:
trycycler cluster --reads reads.fastq.gz --assemblies assemblies/*.fasta --out_dir trycycler
Which produced this output.
A whopping 69 clusters were made, and the tree looks hideous:
This dataset was a bit of a trick – it's unusable rubbish.
- Home
- Software requirements
- Installation
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How to run Trycycler
- Quick start
- Step 1: Generating assemblies
- Step 2: Clustering contigs
- Step 3: Reconciling contigs
- Step 4: Multiple sequence alignment
- Step 5: Partitioning reads
- Step 6: Generating a consensus
- Step 7: Polishing after Trycycler
- Illustrated pipeline overview
- Demo datasets
- Implementation details
- FAQ and miscellaneous tips
- Other pages
- Guide to bacterial genome assembly (choose your own adventure)
- Accuracy vs depth