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The current delta-cq vignette shows how different forms of qPCR data can be incorporated and analysed by tidyqpcr but is not a good example of plate design according to the tidyqpcr ethos. A new vignette going from raw qpcr machine data to publishable results with MIQE compliant, tidy plate design would be a perfect stepping stone for new users.
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I agree this would be good. Let's design an experiment for this, just 2 conditions, 3 tech reps, 2 bio reps (or more), correct controls, that fits on a 96-well plate.
There is a lot of old RNA extracted from Heat-shocked yeast (by me in 2018) in the -80 freezer. If that RNA is still ok, then we could use it. Or do a fresh "quick" experiment.
The current delta-cq vignette shows how different forms of qPCR data can be incorporated and analysed by tidyqpcr but is not a good example of plate design according to the tidyqpcr ethos. A new vignette going from raw qpcr machine data to publishable results with MIQE compliant, tidy plate design would be a perfect stepping stone for new users.
The text was updated successfully, but these errors were encountered: