The plugin can work with multi-channel multi-frame 3D images in *.lsm format obtained from a laser scanning microscope. The main functionality of the plugin includes:
- Assembly and visualization of images.
- Improve image quality (elimination of shear and overlap, application of the anisotropic diffusion algorithm).
- Linear operations with intensity values from different channels.
- Detection a 2D leaf surface.
- Application of the segmentation algorithm for cellular and nuclear layers.
- Manual correction of segmented regions.
- Marking of cell groups.
- Calculation of volumes for cells and nuclei.
- Comparison of cells and nuclei by mutual arrangement.
- Download images in tiff format for single-channel processing (multiple images for each channel can be downloaded).
- Saving results in the form of tables and intermediate images, obtained at any stage of processing.