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RNA Velocity #39

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FionaMoon opened this issue Jun 4, 2022 · 0 comments
Open

RNA Velocity #39

FionaMoon opened this issue Jun 4, 2022 · 0 comments

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@FionaMoon
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Hi,
I'm new to kallistobustools and I want to use this powerful tool to pre-processing 10xscRNA-seq data for RNA Velocity.
I've read the Tutorials of this tool but still confused with some details.

  1. In kb ref，-d {human,mouse,linnarsson}, I could understand that -d {human,mouse} are index for different species, but what's linnarsson for ?
  2. There are 4 kind of workflows to choose --workflow {standard,lamanno,nucleus,kite}. nucleus for single-nucleus RNA-seq, kite for multi-model data. But what standard & lamanno for? Which workflow is best for 10xscRNA-seq?

Thank you inadvance!

Fiona
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@FionaMoon