From 65f1032913b48b39471679c26ac1a95a3d8d4f01 Mon Sep 17 00:00:00 2001 From: maxulysse Date: Fri, 16 Feb 2024 16:55:27 +0100 Subject: [PATCH 001/292] copy previous dev branch --- CHANGELOG.md | 4 +- CITATIONS.md | 2 +- LICENSE | 2 +- README.md | 66 ++- assets/adaptivecard.json | 2 +- assets/email_template.html | 169 ++++-- assets/email_template.txt | 10 +- assets/methods_description_template.yml | 8 +- assets/multiqc_config.yml | 8 +- assets/samplesheet.csv | 6 +- assets/samplesheet_HG002.csv | 4 + assets/samplesheet_HG002_hg19.csv | 5 + assets/samplesheet_HG002_hg38.csv | 5 + assets/samplesheet_SEQC2.csv | 3 + assets/schema_input.json | 55 +- assets/sendmail_template.txt | 6 +- assets/slackreport.json | 2 +- assets/svync/default.yaml | 0 assets/svync/delly.yaml | 69 +++ assets/svync/gridss.yaml | 65 ++ assets/svync/manta.yaml | 66 +++ assets/svync/smoove.yaml | 61 ++ bin/add_svtype.py | 61 ++ bin/annotate_repeat_regions.py | 71 +++ bin/check_samplesheet.py | 337 ++++------- bin/convertInversion.py | 278 +++++++++ bin/id_annotation.py | 52 ++ bin/parse_vcf.py | 50 ++ bin/simple_event_annotator.R | 59 ++ conf/base.config | 10 +- conf/igenomes.config | 372 +----------- conf/modules.config | 182 +++++- conf/test_data.config | 347 +++++++++++ conf/test_full.config | 2 +- conf/test_hg19.config | 42 ++ conf/test_hg37.config | 43 ++ conf/test_hg38.config | 53 ++ conf/test_hg38_somatic.config | 39 ++ conf/truth.config | 24 + docs/README.md | 4 +- docs/output.md | 2 +- docs/usage.md | 12 +- lib/NfcoreSchema.groovy | 528 +++++++++++++++++ lib/WorkflowMain.groovy | 18 +- lib/WorkflowVariantbenchmarking.groovy | 4 +- main.nf | 21 +- modules.json | 81 ++- modules/local/addhead.nf | 38 ++ modules/local/awk_sort.nf | 50 ++ modules/local/bamsurgeon_evaluator.nf | 42 ++ modules/local/bcftools_view.nf | 48 ++ modules/local/bgzip_tabix.nf | 47 ++ modules/local/extract_main.nf | 33 ++ modules/local/gridss_annotation.nf | 60 ++ modules/local/main_chroms.nf | 34 ++ modules/local/samplesheet_check.nf | 4 +- modules/local/truvari_phab.nf | 42 ++ modules/local/truvari_refine.nf | 38 ++ modules/local/vcf_genotype_annotator.nf | 36 ++ modules/local/vcfdist.nf | 41 ++ modules/local/wittyer.nf | 53 ++ .../nf-core/bcftools/annotate/environment.yml | 7 + modules/nf-core/bcftools/annotate/main.nf | 63 ++ modules/nf-core/bcftools/annotate/meta.yml | 56 ++ modules/nf-core/bcftools/isec/environment.yml | 7 + modules/nf-core/bcftools/isec/main.nf | 33 ++ modules/nf-core/bcftools/isec/meta.yml | 52 ++ .../nf-core/bcftools/isec/tests/main.nf.test | 44 ++ .../bcftools/isec/tests/main.nf.test.snap | 25 + .../bcftools/isec/tests/nextflow.config | 3 + modules/nf-core/bcftools/isec/tests/tags.yml | 2 + modules/nf-core/bcftools/norm/environment.yml | 7 + modules/nf-core/bcftools/norm/main.nf | 63 ++ modules/nf-core/bcftools/norm/meta.yml | 61 ++ .../nf-core/bcftools/reheader/environment.yml | 7 + modules/nf-core/bcftools/reheader/main.nf | 68 +++ modules/nf-core/bcftools/reheader/meta.yml | 63 ++ .../bcftools/reheader/tests/bcf.config | 4 + .../bcftools/reheader/tests/main.nf.test | 197 +++++++ .../bcftools/reheader/tests/main.nf.test.snap | 166 ++++++ .../nf-core/bcftools/reheader/tests/tags.yml | 2 + .../bcftools/reheader/tests/vcf.config | 4 + .../bcftools/reheader/tests/vcf.gz.config | 4 + modules/nf-core/bcftools/sort/environment.yml | 7 + modules/nf-core/bcftools/sort/main.nf | 63 ++ modules/nf-core/bcftools/sort/meta.yml | 42 ++ .../nf-core/bcftools/stats/environment.yml | 7 + modules/nf-core/bcftools/stats/main.nf | 60 ++ modules/nf-core/bcftools/stats/meta.yml | 77 +++ modules/nf-core/bcftools/view/environment.yml | 7 + modules/nf-core/bcftools/view/main.nf | 55 ++ modules/nf-core/bcftools/view/meta.yml | 64 ++ .../nf-core/bcftools/view/tests/main.nf.test | 103 ++++ .../bcftools/view/tests/main.nf.test.snap | 45 ++ .../bcftools/view/tests/nextflow.config | 3 + modules/nf-core/bcftools/view/tests/tags.yml | 2 + modules/nf-core/bedgovcf/environment.yml | 7 + modules/nf-core/bedgovcf/main.nf | 49 ++ modules/nf-core/bedgovcf/meta.yml | 57 ++ modules/nf-core/bedgovcf/tests/main.nf.test | 77 +++ .../nf-core/bedgovcf/tests/main.nf.test.snap | 60 ++ modules/nf-core/bedgovcf/tests/tags.yml | 2 + .../nf-core/bedtools/coverage/environment.yml | 7 + modules/nf-core/bedtools/coverage/main.nf | 39 ++ modules/nf-core/bedtools/coverage/meta.yml | 57 ++ .../bedtools/genomecov/environment.yml | 7 + modules/nf-core/bedtools/genomecov/main.nf | 70 +++ modules/nf-core/bedtools/genomecov/meta.yml | 59 ++ .../bedtools/intersect/environment.yml | 7 + modules/nf-core/bedtools/intersect/main.nf | 59 ++ modules/nf-core/bedtools/intersect/meta.yml | 59 ++ .../nf-core/bedtools/merge/environment.yml | 7 + modules/nf-core/bedtools/merge/main.nf | 47 ++ modules/nf-core/bedtools/merge/meta.yml | 45 ++ .../nf-core/bedtools/subtract/environment.yml | 7 + modules/nf-core/bedtools/subtract/main.nf | 50 ++ modules/nf-core/bedtools/subtract/meta.yml | 45 ++ .../bedtools/unionbedg/environment.yml | 7 + modules/nf-core/bedtools/unionbedg/main.nf | 50 ++ modules/nf-core/bedtools/unionbedg/meta.yml | 52 ++ .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../custom/dumpsoftwareversions/meta.yml | 2 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 +- .../nf-core/manta/convertinversion/main.nf | 36 ++ .../nf-core/manta/convertinversion/meta.yml | 46 ++ modules/nf-core/multiqc/environment.yml | 4 +- modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 3 +- .../nf-core/survivor/filter/environment.yml | 7 + modules/nf-core/survivor/filter/main.nf | 64 ++ modules/nf-core/survivor/filter/meta.yml | 58 ++ .../nf-core/survivor/stats/environment.yml | 7 + modules/nf-core/survivor/stats/main.nf | 54 ++ modules/nf-core/survivor/stats/meta.yml | 52 ++ .../nf-core/survivor/stats/tests/main.nf.test | 71 +++ .../survivor/stats/tests/main.nf.test.snap | 34 ++ modules/nf-core/survivor/stats/tests/tags.yml | 2 + .../svanalyzer/svbenchmark/environment.yml | 10 + .../nf-core/svanalyzer/svbenchmark/main.nf | 65 ++ .../nf-core/svanalyzer/svbenchmark/meta.yml | 80 +++ .../svanalyzer/svbenchmark/tests/main.nf.test | 85 +++ .../svbenchmark/tests/main.nf.test.snap | 188 ++++++ .../svanalyzer/svbenchmark/tests/tags.yml | 2 + modules/nf-core/svync/environment.yml | 9 + modules/nf-core/svync/main.nf | 40 ++ modules/nf-core/svync/meta.yml | 59 ++ modules/nf-core/svync/tests/main.nf.test | 84 +++ modules/nf-core/svync/tests/main.nf.test.snap | 55 ++ modules/nf-core/svync/tests/tags.yml | 2 + modules/nf-core/tabix/bgzip/environment.yml | 7 + modules/nf-core/tabix/bgzip/main.nf | 54 ++ modules/nf-core/tabix/bgzip/meta.yml | 52 ++ .../nf-core/tabix/bgziptabix/environment.yml | 7 + modules/nf-core/tabix/bgziptabix/main.nf | 47 ++ modules/nf-core/tabix/bgziptabix/meta.yml | 53 ++ modules/nf-core/tabix/tabix/environment.yml | 7 + modules/nf-core/tabix/tabix/main.nf | 42 ++ modules/nf-core/tabix/tabix/meta.yml | 49 ++ modules/nf-core/truvari/bench/environment.yml | 7 + modules/nf-core/truvari/bench/main.nf | 61 ++ modules/nf-core/truvari/bench/meta.yml | 111 ++++ nextflow.config | 74 ++- nextflow_schema.json | 558 +++++++++--------- pyproject.toml | 17 +- subworkflows/local/germline_benchmark.nf | 103 ++++ .../local/germline_benchmark_backup.nf | 162 +++++ subworkflows/local/input_check.nf | 58 +- subworkflows/local/prepare_regions.nf | 44 ++ subworkflows/local/prepare_vcfs_test.nf | 131 ++++ subworkflows/local/prepare_vcfs_truth.nf | 99 ++++ subworkflows/local/report_vcf_statistics.nf | 52 ++ subworkflows/local/somatic_benchmark.nf | 49 ++ subworkflows/local/vcf_conversion.nf | 126 ++++ subworkflows/local/visualizations.nf | 35 ++ workflows/variantbenchmarking.nf | 140 ++++- 177 files changed, 8407 insertions(+), 1203 deletions(-) create mode 100644 assets/samplesheet_HG002.csv create mode 100644 assets/samplesheet_HG002_hg19.csv create mode 100644 assets/samplesheet_HG002_hg38.csv create mode 100644 assets/samplesheet_SEQC2.csv create mode 100644 assets/svync/default.yaml create mode 100644 assets/svync/delly.yaml create mode 100644 assets/svync/gridss.yaml create mode 100644 assets/svync/manta.yaml create mode 100644 assets/svync/smoove.yaml create mode 100644 bin/add_svtype.py create mode 100644 bin/annotate_repeat_regions.py create mode 100644 bin/convertInversion.py create mode 100644 bin/id_annotation.py create mode 100644 bin/parse_vcf.py create mode 100644 bin/simple_event_annotator.R create mode 100644 conf/test_data.config create mode 100644 conf/test_hg19.config create mode 100644 conf/test_hg37.config create mode 100644 conf/test_hg38.config create mode 100644 conf/test_hg38_somatic.config create mode 100644 conf/truth.config create mode 100644 lib/NfcoreSchema.groovy mode change 100755 => 100644 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create mode 100644 subworkflows/local/report_vcf_statistics.nf create mode 100644 subworkflows/local/somatic_benchmark.nf create mode 100644 subworkflows/local/vcf_conversion.nf create mode 100644 subworkflows/local/visualizations.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index e86fa48..8f39c6e 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,11 +1,11 @@ -# nf-core/variantbenchmarking: Changelog +# nf-core/benchmark: Changelog The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). ## v1.0dev - [date] -Initial release of nf-core/variantbenchmarking, created with the [nf-core](https://nf-co.re/) template. +Initial release of nf-core/benchmark, created with the [nf-core](https://nf-co.re/) template. ### `Added` diff --git a/CITATIONS.md b/CITATIONS.md index 28d5f49..2bc3e86 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,4 +1,4 @@ -# nf-core/variantbenchmarking: Citations +# nf-core/benchmark: Citations ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) diff --git a/LICENSE b/LICENSE index e48cb19..0aefc2e 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) Kübra Narcı +Copyright (c) kuebra.narci@dkfz.de Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index d9d4aaa..090c8b2 100644 --- a/README.md +++ b/README.md @@ -1,23 +1,19 @@ -

- - - nf-core/variantbenchmarking - -

-[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +# ![nf-core/svbench](docs/images/nf-core-svbench_logo_light.png#gh-light-mode-only) + +[![GitHub Actions CI Status](https://github.com/nf-core/svbench/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/svbench/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/svbench/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/svbench/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/svbench/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/variantbenchmarking) +[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/svbench) -[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23benchmark-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/svbench)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) ## Introduction -**nf-core/variantbenchmarking** is a bioinformatics pipeline that ... +**nf-core/svbench** is a bioinformatics pipeline that ... -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +1. Standardization of SVs in test VCF files +2. Normalization of SVs in test VCF files +3. Normalization of SVs in truth VCF files +4. SV stats and histograms +5. Germline benchmarking of SVs +6. Somatic benchmarking of SVs +7. Final report and comparisons ## Usage -> [!NOTE] -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. +:::note +If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how +to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) +with `-profile test` before running the workflow on actual data. +::: + +Supported SV callers: Manta, SVaba, Dragen, Delly, Lumpy .. +Available Truth samples: HG002, SEQC2 Now, you can run the pipeline using: @@ -58,27 +64,29 @@ Now, you can run the pipeline using: ```bash -nextflow run nf-core/variantbenchmarking \ +nextflow run nf-core/svbench \ -profile \ --input samplesheet.csv \ --outdir ``` -> [!WARNING] -> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; -> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). +:::warning +Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those +provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; +see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). +::: -For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). +For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/svbench/usage) and the [parameter documentation](https://nf-co.re/svbench/parameters). ## Pipeline output -To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page. +To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/svbench/results) tab on the nf-core website pipeline page. For more details about the output files and reports, please refer to the -[output documentation](https://nf-co.re/variantbenchmarking/output). +[output documentation](https://nf-co.re/svbench/output). ## Credits -nf-core/variantbenchmarking was originally written by Kübra Narcı. +nf-core/svbench was originally written by kuebra.narci@dkfz.de. We thank the following people for their extensive assistance in the development of this pipeline: @@ -88,12 +96,12 @@ We thank the following people for their extensive assistance in the development If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). -For further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)). +For further information or help, don't hesitate to get in touch on the [Slack `#svbench` channel](https://nfcore.slack.com/channels/svbench) (you can join with [this invite](https://nf-co.re/join/slack)). ## Citations - + diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json index 4b5f7c5..4f89163 100644 --- a/assets/adaptivecard.json +++ b/assets/adaptivecard.json @@ -17,7 +17,7 @@ "size": "Large", "weight": "Bolder", "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", - "text": "nf-core/variantbenchmarking v${version} - ${runName}", + "text": "nf-core/benchmark v${version} - ${runName}", "wrap": true }, { diff --git a/assets/email_template.html b/assets/email_template.html index ee343c4..323a139 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,53 +1,134 @@ - - - - + + + + - - nf-core/variantbenchmarking Pipeline Report - - -
+ + nf-core/benchmark Pipeline Report + + +
+ - +

nf-core/benchmark v${version}

+

Run Name: $runName

-

nf-core/variantbenchmarking ${version}

-

Run Name: $runName

- -<% if (!success){ - out << """ -
-

nf-core/variantbenchmarking execution completed unsuccessfully!

-

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

+ <% if (!success){ out << """ +
+

+ nf-core/benchmark execution completed unsuccessfully! +

+

+ The exit status of the task that caused the workflow execution to fail + was: $exitStatus. +

The full error message was:

- 
${errorReport}
-
- """ -} else { - out << """ -
- nf-core/variantbenchmarking execution completed successfully! -
- """ -} -%> + 
+${errorReport}
+
+ """ } else { out << """ +
+ nf-core/benchmark execution completed successfully! +
+ """ } %> -

The workflow was completed at $dateComplete (duration: $duration)

-

The command used to launch the workflow was as follows:

-
$commandLine
+

+ The workflow was completed at $dateComplete (duration: + $duration) +

+

The command used to launch the workflow was as follows:

+ 
+$commandLine
-

Pipeline Configuration:

- - - <% out << summary.collect{ k,v -> "" }.join("\n") %> - -
$k
$v
+

Pipeline Configuration:

+ + + <% out << summary.collect{ k,v -> " + + + + + " }.join("\n") %> + +
+ $k + + 
$v
+
-

nf-core/variantbenchmarking

-

https://github.com/nf-core/variantbenchmarking

- -
- - +

nf-core/benchmark

+

+ https://github.com/nf-core/benchmark +

+
+ diff --git a/assets/email_template.txt b/assets/email_template.txt index e9ad84c..4acfb7f 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,15 +4,15 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/variantbenchmarking ${version} + nf-core/benchmark v${version} ---------------------------------------------------- Run Name: $runName <% if (success){ - out << "## nf-core/variantbenchmarking execution completed successfully! ##" + out << "## nf-core/benchmark execution completed successfully! ##" } else { out << """#################################################### -## nf-core/variantbenchmarking execution completed unsuccessfully! ## +## nf-core/benchmark execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: $exitStatus. The full error message was: @@ -35,5 +35,5 @@ Pipeline Configuration: <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> -- -nf-core/variantbenchmarking -https://github.com/nf-core/variantbenchmarking +nf-core/benchmark +https://github.com/nf-core/benchmark diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index 1e3c455..35dd7dd 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -1,13 +1,13 @@ -id: "nf-core-variantbenchmarking-methods-description" +id: "nf-core-benchmark-methods-description" description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "nf-core/variantbenchmarking Methods Description" -section_href: "https://github.com/nf-core/variantbenchmarking" +section_name: "nf-core/benchmark Methods Description" +section_href: "https://github.com/nf-core/benchmark" plot_type: "html" ## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

Methods

-

Data was processed using nf-core/variantbenchmarking v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

+

Data was processed using nf-core/benchmark v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

${workflow.commandLine}

${tool_citations}

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index d26a71b..f195fd3 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,13 +1,13 @@ report_comment: > - This report has been generated by the nf-core/variantbenchmarking + This report has been generated by the nf-core/benchmark analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: - "nf-core-variantbenchmarking-methods-description": + "nf-core-benchmark-methods-description": order: -1000 software_versions: order: -1001 - "nf-core-variantbenchmarking-summary": + "nf-core-benchmark-summary": order: -1002 export_plots: true diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 5f653ab..d7db7d3 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,3 @@ -sample,fastq_1,fastq_2 -SAMPLE_PAIRED_END,/path/to/fastq/files/AEG588A1_S1_L002_R1_001.fastq.gz,/path/to/fastq/files/AEG588A1_S1_L002_R2_001.fastq.gz -SAMPLE_SINGLE_END,/path/to/fastq/files/AEG588A4_S4_L003_R1_001.fastq.gz, +sample,test_vcf,truth_vcf,caller,type +HG002,"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/HG002.svaba.germline.sv.convBNDtoDEL.vcf","/Users/w620-admin/Desktop/nf-core/dataset/hg37/GIAB_Assemblytics_structural_variants_only_160618/hg002.Assemblytics_structural_variants.sorted.vcf.gz",svaba,sv +HG003,"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/HG003.svaba.germline.sv.convBNDtoDEL.vcf","/Users/w620-admin/Desktop/nf-core/dataset/hg37/GIAB_Assemblytics_structural_variants_only_160618/hg003.Assemblytics_structural_variants.sorted.vcf.gz",svaba,sv diff --git a/assets/samplesheet_HG002.csv b/assets/samplesheet_HG002.csv new file mode 100644 index 0000000..db4ccca --- /dev/null +++ b/assets/samplesheet_HG002.csv @@ -0,0 +1,4 @@ +test_vcf,caller +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_delly_SV_hg19.vcf.gz",delly +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.vcf.gz",lumpy +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype.vcf",manta \ No newline at end of file diff --git a/assets/samplesheet_HG002_hg19.csv b/assets/samplesheet_HG002_hg19.csv new file mode 100644 index 0000000..d1d8dba --- /dev/null +++ b/assets/samplesheet_HG002_hg19.csv @@ -0,0 +1,5 @@ +test_vcf,caller +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_DRAGEN_SV_hg19.vcf.gz",dragen +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_delly_SV_hg19.vcf.gz",delly +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.vcf.gz",lumpy +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype.vcf",manta \ No newline at end of file diff --git a/assets/samplesheet_HG002_hg38.csv b/assets/samplesheet_HG002_hg38.csv new file mode 100644 index 0000000..95cbeef --- /dev/null +++ b/assets/samplesheet_HG002_hg38.csv @@ -0,0 +1,5 @@ +test_vcf,caller +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.vcf.gz",lumpy +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/manta.HG002.vcf.gz",manta +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Ashkenazim_unnanotated/Ashkenazim_HG002.filtered.sv.vcf.gz",merged + diff --git a/assets/samplesheet_SEQC2.csv b/assets/samplesheet_SEQC2.csv new file mode 100644 index 0000000..5b64aed --- /dev/null +++ b/assets/samplesheet_SEQC2.csv @@ -0,0 +1,3 @@ +test_vcf,caller +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/test/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-Strelka.indel.vcf.gz",strelka +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/test/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-MuTect2.vcf.gz",mutect2 diff --git a/assets/schema_input.json b/assets/schema_input.json index c4f8dd9..5746c3f 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,36 +1,23 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/assets/schema_input.json", - "title": "nf-core/variantbenchmarking pipeline - params.input schema", - "description": "Schema for the file provided with params.input", - "type": "array", - "items": { - "type": "object", - "properties": { - "sample": { - "type": "string", - "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" - }, - "fastq_1": { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$", - "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" - }, - "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] - } - }, - "required": ["sample", "fastq_1"] - } + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/benchmark/master/assets/schema_input.json", + "title": "nf-core/benchmark pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "test_vcf": { + "type": "string", + "pattern": "", + "errorMessage": "Test VCF must be provided, cannot contain spaces and must have extension '.vcf.gz'" + }, + "caller": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Name of the variant caller used to generate test file" + } + }, + "required": ["test_vcf","caller"] + } } diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 6ab0eb8..22c306e 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -9,12 +9,12 @@ Content-Type: text/html; charset=utf-8 $email_html --nfcoremimeboundary -Content-Type: image/png;name="nf-core-variantbenchmarking_logo.png" +Content-Type: image/png;name="nf-core-benchmark_logo.png" Content-Transfer-Encoding: base64 Content-ID: -Content-Disposition: inline; filename="nf-core-variantbenchmarking_logo_light.png" +Content-Disposition: inline; filename="nf-core-benchmark_logo_light.png" -<% out << new File("$projectDir/assets/nf-core-variantbenchmarking_logo_light.png"). +<% out << new File("$projectDir/assets/nf-core-benchmark_logo_light.png"). bytes. encodeBase64(). toString(). diff --git a/assets/slackreport.json b/assets/slackreport.json index 9e94a79..d2d6f69 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/variantbenchmarking ${version} - ${runName}", + "author_name": "nf-core/svbench ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/assets/svync/default.yaml b/assets/svync/default.yaml new file mode 100644 index 0000000..e69de29 diff --git a/assets/svync/delly.yaml b/assets/svync/delly.yaml new file mode 100644 index 0000000..f820a1d --- /dev/null +++ b/assets/svync/delly.yaml @@ -0,0 +1,69 @@ +id: delly_$INFO/SVTYPE +alt: + BND: TRA +info: + CALLER: + value: delly + number: 1 + type: string + description: The caller used to determine this variant + SVLEN: + value: ~sub:$INFO/END,$POS + number: 1 + type: integer + description: The length of the structural variant + alts: + DEL: -~sub:$INFO/END,$POS + INS: $INFO/SVLEN + TRA: 1 + CIEND: + value: $INFO/CIEND + number: 2 + type: integer + description: PE confidence interval around END + CIPOS: + value: $INFO/CIPOS + number: 2 + type: integer + description: PE confidence interval around POS + SVTYPE: + value: $INFO/SVTYPE + number: 1 + type: string + description: Type of structural variant + CHR2: + value: + number: 1 + type: string + description: Chromosome for second position + alts: + TRA: $INFO/CHR2 + END: + value: $INFO/END + number: 1 + type: integer + description: End position of the structural variant + alts: + TRA: $INFO/POS2 + IMPRECISE: + value: $INFO/IMPRECISE + number: 0 + type: flag + description: Imprecise structural variation +format: + GT: + value: $FORMAT/GT + number: 1 + type: string + description: Genotype + PE: + value: $FORMAT/DR,$FORMAT/DV + number: 2 + type: integer + description: Paired-read support for the ref and alt alleles in the order listed + SR: + value: $FORMAT/RR,$FORMAT/RV + number: 2 + type: integer + description: Split-read support for the ref and alt alleles in the order listed + diff --git a/assets/svync/gridss.yaml b/assets/svync/gridss.yaml new file mode 100644 index 0000000..62c8c8a --- /dev/null +++ b/assets/svync/gridss.yaml @@ -0,0 +1,65 @@ +id: gridss_$INFO/SVTYPE +info: + CALLER: + value: gridss + number: 1 + type: string + description: The caller used to determine this variant + SVLEN: + value: ~sub:$INFO/END,$POS + number: 1 + type: integer + description: The length of the structural variant + alts: + BND: + TRA: 0 + DEL: -~sub:$INFO/END,$POS + CIEND: + value: $INFO/CIRPOS + number: 2 + type: integer + description: PE confidence interval around END + CIPOS: + value: $INFO/CIPOS + number: 2 + type: integer + description: PE confidence interval around POS + SVTYPE: + value: $INFO/SVTYPE + number: 1 + type: string + description: Type of structural variant + CHR2: + value: + number: 1 + type: string + description: Chromosome for second position + alts: + TRA: $INFO/CHR2 + END: + value: $INFO/END + number: 1 + type: integer + description: End position of the structural variant + IMPRECISE: + value: $INFO/IMPRECISE + number: 0 + type: flag + description: Imprecise structural variation +format: + GT: + value: $FORMAT/GT + number: 1 + type: string + description: Genotype + PE: + value: $FORMAT/REFPAIR,$FORMAT/RP + number: 2 + type: integer + description: Paired-read support for the ref and alt alleles in the order listed + SR: + value: .,$FORMAT/SR + number: 2 + type: integer + description: Split-read support for the ref and alt alleles in the order listed + diff --git a/assets/svync/manta.yaml b/assets/svync/manta.yaml new file mode 100644 index 0000000..eaa5869 --- /dev/null +++ b/assets/svync/manta.yaml @@ -0,0 +1,66 @@ +id: manta_$INFO/SVTYPE +info: + CALLER: + value: manta + number: 1 + type: string + description: The caller used to determine this variant + SVLEN: + value: $INFO/SVLEN + number: 1 + type: integer + description: The length of the structural variant + alts: + INS: ~sum:~len:LEFT_SVINSSEQ,~len:RIGHT_SVINSSEQ + TRA: 1 + CIEND: + value: $INFO/CIEND + number: 2 + type: integer + description: PE confidence interval around END + CIPOS: + value: $INFO/CIPOS + number: 2 + type: integer + description: PE confidence interval around POS + SVTYPE: + value: $INFO/SVTYPE + number: 1 + type: string + description: Type of structural variant + CHR2: + value: + number: 1 + type: string + description: Chromosome for second position + alts: + TRA: $INFO/CHR2 + END: + value: $INFO/END + number: 1 + type: integer + description: End position of the structural variant + alts: + TRA: $INFO/POS2 + IMPRECISE: + value: $INFO/IMPRECISE + number: 0 + type: flag + description: Imprecise structural variation +format: + GT: + value: $FORMAT/GT + number: 1 + type: string + description: Genotype + PE: + value: $FORMAT/PR + number: 2 + type: integer + description: Paired-read support for the ref and alt alleles in the order listed + SR: + value: $FORMAT/SR + number: 2 + type: integer + description: Split-read support for the ref and alt alleles in the order listed + diff --git a/assets/svync/smoove.yaml b/assets/svync/smoove.yaml new file mode 100644 index 0000000..ee277a7 --- /dev/null +++ b/assets/svync/smoove.yaml @@ -0,0 +1,61 @@ +id: smoove_$INFO/SVTYPE +info: + CALLER: + value: smoove + number: 1 + type: string + description: The caller used to determine this variant + SVLEN: + value: $INFO/SVLEN + number: 1 + type: integer + description: The length of the structural variant + CIEND: + value: $INFO/CIEND + number: 2 + type: integer + description: PE confidence interval around END + CIPOS: + value: $INFO/CIPOS + number: 2 + type: integer + description: PE confidence interval around POS + SVTYPE: + value: $INFO/SVTYPE + number: 1 + type: string + description: Type of structural variant + CHR2: + value: + number: 1 + type: string + description: Chromosome for second position + alts: + TRA: $INFO/CHR2 + END: + value: $INFO/END + number: 1 + type: integer + description: End position of the structural variant + IMPRECISE: + value: $INFO/IMPRECISE + number: 0 + type: flag + description: Imprecise structural variation +format: + GT: + value: $FORMAT/GT + number: 1 + type: string + description: Genotype + PE: + value: .,$FORMAT/PE + number: 2 + type: integer + description: Paired-read support for the ref and alt alleles in the order listed + SR: + value: .,$FORMAT/SR + number: 2 + type: integer + description: Split-read support for the ref and alt alleles in the order listed + diff --git a/bin/add_svtype.py b/bin/add_svtype.py new file mode 100644 index 0000000..d93981e --- /dev/null +++ b/bin/add_svtype.py @@ -0,0 +1,61 @@ +import argparse +import pysam +import logging +import os +import subprocess as sp + +logging.basicConfig( + format='%(levelname)-7s | %(asctime)s | %(message)s', + datefmt='%H:%M:%S') +logger = logging.getLogger(__name__) +logger.setLevel(logging.INFO) + + +parser = argparse.ArgumentParser() +parser.add_argument('--graph', required=True, help='Input VCF') +parser.add_argument('--svlength', type=int, required=False, help='SV Lenght', default=50) +args = parser.parse_args() + + + +in_vcf = pysam.VariantFile(args.graph) +out_name = os.path.basename(args.graph) +if out_name.endswith('.gz'): + out_name = out_name[:-3] +if out_name.endswith('.vcf'): + out_name = out_name[:-4] + +anno_header = in_vcf.header +logger.info('Writing Header') +anno_header.info.add("SVTYPE","1","String","Type of structural variant") +anno_header.info.add("SVLEN",".","Integer","Difference in length between the REF and ALT alleles") + +anno_vcf = pysam.VariantFile('{}.annotated.vcf.gz'.format(out_name), 'w', header=anno_header) + +logger.info('Adding SVTYE/SVLEN to VCF') +counter=0 +for v in in_vcf: + svlen=(len(v.alts[0])-len(v.ref)) + limit=args.svlength + + if (svlen < -1*limit): + v.info.update({'SVTYPE':"DEL"}) + v.info.update({'SVLEN':svlen}) + anno_vcf.write(v) + counter=counter+1 + elif (svlen > limit): + v.info.update({'SVTYPE':"INS"}) + v.info.update({'SVLEN':svlen}) + anno_vcf.write(v) + counter=counter+1 + else: + anno_vcf.write(v) + +in_vcf.close() +anno_vcf.close() +logger.info('Finished adding SVTYE/SVLEN to VCF: {}'.format(counter)) + +sp.run(['tabix', anno_vcf.filename]) +logger.info('VCF indexed') + +logger.info('DONE') diff --git a/bin/annotate_repeat_regions.py b/bin/annotate_repeat_regions.py new file mode 100644 index 0000000..c92bcaa --- /dev/null +++ b/bin/annotate_repeat_regions.py @@ -0,0 +1,71 @@ +import pysam +import pandas as pd +import numpy as np +import matplotlib.pyplot as plt +import argparse +import os + +parser = argparse.ArgumentParser() +parser.add_argument('--vcf', required=True, help='Input VCF') +parser.add_argument('--repeat', required=True, help='Simple Repeat regions') +parser.add_argument('--repmask', required=True, help='Repeat Mask regions') +parser.add_argument('--segdup', required=True, help='Segmental Duplication regions') + +args = parser.parse_args + +def print_AF(in_vcf,samplerep,repeatmask,segdup): + in_file = pysam.VariantFile(in_vcf) + out_name = os.path.basename(in_vcf) + if out_name.endswith('.gz'): + out_name = out_name[:-3] + if out_name.endswith('.vcf'): + out_name = out_name[:-4] + + with open(out_name + ".txt","w") as out_file: + + print('SV_Type', 'SV', 'Length' , 'AF', 'Simple_Repeat','Repeat_Name','Repeat_Class','Segmental_Dup', file=out_file) + for v in in_vcf: + temp1=samplerep[(samplerep['chrom']== v.chrom) &( (( v.start > samplerep['chromStart']-1 ) & ( v.start < samplerep['chromEnd'] +1 ) )| (( v.stop > samplerep['chromStart'] -1) & ( v.stop < samplerep['chromEnd'] +1 ))) ] + + if len(temp1) > 0: + simple_repeat=temp1.iloc[0].period + else: + simple_repeat='None' + + temp2=repeatmask[(repeatmask['genoName']== v.chrom) &( (( v.start > repeatmask['genoStart']-1 ) & ( v.start < repeatmask['genoEnd'] +1 ) )| (( v.stop > repeatmask['genoStart'] -1) & ( v.stop < repeatmask['genoEnd'] +1 ))) ] + + if len(temp2) > 0: + repname=temp2.iloc[0].repName + repclass=temp2.iloc[0].repClass + else: + repname='None' + repclass='None' + + temp3=segdup[(segdup['chrom']== v.chrom) &( (( v.start > segdup['chromStart']-1 ) & ( v.start < segdup['chromEnd'] +1 ) )| (( v.stop > segdup['chromStart'] -1) & ( v.stop < segdup['chromEnd'] +1 ))) ] + if len(temp3) > 0: + segdups=temp3.iloc[0].fracMatch + else: + segdups='None' + + + if len(v.ref) - len(v.alts[0]) > 49: + if len(v.ref) - len(v.alts[0]) < 1001: + print('DEL', v.ref, len(v.alts[0])- len(v.ref) , v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) + else: + print('DEL', '...', len(v.alts[0])- len(v.ref) , v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) + + elif len(v.alts[0]) - len(v.ref) > 49: + if len(v.ref) - len(v.alts[0]) < 1001: + print('INS', v.alts[0],len(v.alts[0]) - len(v.ref), v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) + else: + print('INS', '...',len(v.alts[0]) - len(v.ref), v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) + + else: + continue + + +repeat_file = pd.read_csv(args.repeat, header=0, sep='\t') +repmask_file = pd.read_csv(args.repmask, header=0, sep='\t') +segdup_file = pd.read_csv(args.segdups, header=0, sep='\t') + +print_AF(args.vcf, repeat_file,repmask_file,segdup_file ) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 4a758fe..5bbe208 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -1,258 +1,125 @@ -#!/usr/bin/env python +#!/usr/bin/env python3 - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging +import os import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. +import errno +import argparse - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" +def parse_args(args=None): + Description = "Reformat samplesheet file and check its contents." + Epilog = "Example usage: python check_samplesheet.py " -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) + parser = argparse.ArgumentParser(description=Description, epilog=Epilog) + parser.add_argument("FILE_IN", help="Input samplesheet file.") + parser.add_argument("FILE_OUT", help="Output file.") + return parser.parse_args(args) -def sniff_format(handle): - """ - Detect the tabular format. +def make_dir(path): + if len(path) > 0: + try: + os.makedirs(path) + except OSError as exception: + if exception.errno != errno.EEXIST: + raise exception - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect +def print_error(error, context="Line", context_str=""): + error_str = f"ERROR: Please check samplesheet -> {error}" + if context != "" and context_str != "": + error_str = f"ERROR: Please check samplesheet -> {error}\n{context.strip()}: '{context_str.strip()}'" + print(error_str) + sys.exit(1) def check_samplesheet(file_in, file_out): """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, + This function checks that the samplesheet follows the following structure: + test_vcf,caller + test1.vcf,manta + test2.vcf,svaba + For an example see: + https://github.com/ghga-de/nf-benchmark/assets/samplesheet.csv + """ - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv + sample_mapping_dict = {} + with open(file_in, "r", encoding='utf-8-sig') as fin: - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") + ## Check header + MIN_COLS = 2 + HEADER = ["test_vcf","caller"] + header = [x.strip('"') for x in fin.readline().strip().split(",")] + if header[: len(HEADER)] != HEADER: + print( + f"ERROR: Please check samplesheet header -> {','.join(header)} != {','.join(HEADER)}" + ) sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) + ## Check caller entries + for line in fin: + if line.strip(): + lspl = [x.strip().strip('"') for x in line.strip().split(",")] + + ## Check valid number of columns per row + if len(lspl) < len(HEADER): + print_error( + f"Invalid number of columns (minimum = {len(HEADER)})!", + "Line", + line, + ) + + num_cols = len([x for x in lspl if x]) + if num_cols < MIN_COLS: + print_error( + f"Invalid number of populated columns (minimum = {MIN_COLS})!", + "Line", + line, + ) + + ## Check caller name entries + test_vcf, caller = lspl[: len(HEADER)] + if caller.find(" ") != -1: + print( + f"WARNING: Spaces have been replaced by underscores for caller: {caller}" + ) + caller = caller.replace(" ", "_") + if not caller: + print_error("Caller entry has not been specified!", "Line", line) + + sample_info = [] ## [test_vcf, caller ] + + sample_info = [test_vcf, caller] + + ## Create caller mapping dictionary = {caller: [[test_vcf, caller ]]} + if caller not in sample_mapping_dict: + sample_mapping_dict[caller] = [sample_info] + else: + if sample_info in sample_mapping_dict[caller]: + print_error("Samplesheet contains duplicate rows!", "Line", line) + else: + sample_mapping_dict[caller].append(sample_info) + + ## Write validated samplesheet with appropriate columns + if len(sample_mapping_dict) > 0: + out_dir = os.path.dirname(file_out) + make_dir(out_dir) + with open(file_out, "w") as fout: + fout.write( + ",".join(["test_vcf","caller"]) + + "\n" + ) + for caller in sorted(sample_mapping_dict.keys()): -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) + for idx, val in enumerate(sample_mapping_dict[caller]): + fout.write(",".join(val) + "\n") + else: + print_error(f"No entries to process!", "Samplesheet: {file_in}") -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) +def main(args=None): + args = parse_args(args) + check_samplesheet(args.FILE_IN, args.FILE_OUT) if __name__ == "__main__": diff --git a/bin/convertInversion.py b/bin/convertInversion.py new file mode 100644 index 0000000..8f70cdf --- /dev/null +++ b/bin/convertInversion.py @@ -0,0 +1,278 @@ +#!/usr/bin/env python2 +# + + +import sys +import gzip +from io import BufferedReader +from subprocess import check_output +from os import path +from os.path import exists + + +class VcfRecord: + + def __init__(self, inline): + tokens = inline.strip().split('\t') + + self.chr = tokens[0] + self.pos = int(tokens[1]) + self.vid = tokens[2] + self.ref = tokens[3] + self.alt = tokens[4] + self.qual = tokens[5] + self.filter = tokens[6] + self.info = tokens[7].split(';') + self.others = "\t".join(tokens[8:]) + + # Create a dictionary for INFO + self.infoDict ={} + for infoItem in self.info: + items = infoItem.split('=') + if len(items) == 1: + self.infoDict[items[0]] = True + elif len(items) > 1: + self.infoDict[items[0]] = items[1] + + self.isINV3 = False + self.isINV5 = False + self.mateChr = "" + self.matePos = -1 + + + def checkInversion(self): + + def getMateInfo(splitChar): + items = self.alt.split(splitChar) + mateInfo = items[1].split(':') + # Assuming that the last item, contains position information + matePos = mateInfo[-1] + # Other items belong to chromosome + self.mateChr = ":".join(mateInfo[:-1]) + self.matePos = int(matePos) + + if self.alt.startswith('['): + getMateInfo('[') + if self.mateChr == self.chr: + self.isINV5 = True + elif self.alt.endswith(']'): + getMateInfo(']') + if self.mateChr == self.chr: + self.isINV3 = True + + + def makeLine(self): + infoStr = ";".join(self.info) + + self.line = "\t".join((self.chr, + str(self.pos), + self.vid, + self.ref, + self.alt, + self.qual, + self.filter, + infoStr, + self.others + ))+"\n" + + +def scanVcf(vcfFile): + + invMateDict = {} + + if vcfFile.endswith('gz'): + gzfp = gzip.open(vcfFile, 'rb') + fpVcf = BufferedReader(gzfp) + else: + fpVcf = open(vcfFile, 'rb') + + for line in fpVcf: + if line[0] == '#': + continue + + vcfRec = VcfRecord(line) + vcfRec.checkInversion() + if vcfRec.isINV3 or vcfRec.isINV5: + if vcfRec.vid in invMateDict: + # update mate INFO + invMateDict[vcfRec.vid] = vcfRec.infoDict + else: + mateId = vcfRec.infoDict["MATEID"] + invMateDict[mateId] = "" + + return invMateDict + + +def getReference(samtools, refFasta, chrom, start, end): + region = "%s:%d-%d" % (chrom, start, end) + samtoolsOut = check_output([samtools, "faidx", refFasta, region]) + refSeq = "" + for seq in samtoolsOut.split('\n'): + if not seq.startswith(">"): + refSeq += seq + + return refSeq.upper() + + +def writeLines(lines): + for line in lines: + sys.stdout.write(line) + + +def convertInversions(samtools, refFasta, vcfFile, invMateDict): + isHeaderInfoAdded = False + isHeaderAltAdded = False + lineBuffer = [] + bufferedChr = "" + bufferedPos = -1 + + if vcfFile.endswith('gz'): + gzfp = gzip.open(vcfFile, 'rb') + fpVcf = BufferedReader(gzfp) + else: + fpVcf = open(vcfFile, 'rb') + + for line in fpVcf: + if line.startswith('#'): + if (not isHeaderInfoAdded) and line.startswith("##FORMAT="): + sys.stdout.write("##INFO=\n") + sys.stdout.write("##INFO=\n") + isHeaderInfoAdded = True + + if (not isHeaderAltAdded) and line.startswith("##ALT="): + sys.stdout.write("##ALT=\n") + isHeaderAltAdded = True + + sys.stdout.write(line) + continue + + vcfRec = VcfRecord(line) + + # skip mate record + if vcfRec.vid in invMateDict: + continue + + vcfRec.checkInversion() + if vcfRec.isINV3 or vcfRec.isINV5: + if vcfRec.isINV5: + # adjust POS for INV5 + vcfRec.pos -= 1 + vcfRec.matePos -= 1 + vcfRec.ref = getReference(samtools, refFasta, + vcfRec.chr, vcfRec.pos, vcfRec.pos) + + # update manta ID + vidSuffix = vcfRec.vid.split("MantaBND")[1] + idx = vidSuffix.rfind(':') + vcfRec.vid = "MantaINV%s" % vidSuffix[:idx] + + # symbolic ALT + vcfRec.alt = "" + + # add END + infoEndStr = "END=%d" % vcfRec.matePos + + newInfo = [infoEndStr] + for infoItem in vcfRec.info: + if infoItem.startswith("SVTYPE"): + # change SVTYPE + newInfo.append("SVTYPE=INV") + # add SVLEN + infoSvLenStr = "SVLEN=%d" % (vcfRec.matePos-vcfRec.pos) + newInfo.append(infoSvLenStr) + + elif infoItem.startswith("CIPOS"): + newInfo.append(infoItem) + + # set CIEND + isImprecise = "IMPRECISE" in vcfRec.infoDict + # for imprecise calls, set CIEND to the mate breakpoint's CIPOS + if isImprecise: + mateId = vcfRec.infoDict["MATEID"] + mateInfoDict = invMateDict[mateId] + infoCiEndStr = "CIEND=%s" % (mateInfoDict["CIPOS"]) + newInfo.append(infoCiEndStr) + # for precise calls, set CIEND w.r.t HOMLEN + else: + if "HOMLEN" in vcfRec.infoDict: + infoCiEndStr = "CIEND=-%s,0" % vcfRec.infoDict["HOMLEN"] + newInfo.append(infoCiEndStr) + + elif infoItem.startswith("HOMSEQ"): + # update HOMSEQ for INV5 + if vcfRec.isINV5: + cipos = vcfRec.infoDict["CIPOS"].split(',') + homSeqStart = vcfRec.pos + int(cipos[0]) + 1 + homSeqEnd = vcfRec.pos + int(cipos[1]) + refSeq = getReference(samtools, refFasta, vcfRec.chr, + homSeqStart, homSeqEnd) + infoHomSeqStr = "HOMSEQ=%s" % refSeq + newInfo.append(infoHomSeqStr) + else: + newInfo.append(infoItem) + + # skip BND-specific tags + elif (infoItem.startswith("MATEID") or + infoItem.startswith("BND_DEPTH") or + infoItem.startswith("MATE_BND_DEPTH")): + continue + + # update event ID + elif infoItem.startswith("EVENT"): + eidSuffix = vcfRec.infoDict["EVENT"].split("MantaBND")[1] + idx = vidSuffix.rfind(':') + infoEventStr = "EVENT=MantaINV%s" % eidSuffix[:idx] + newInfo.append(infoEventStr) + + # apply all other tags + else: + newInfo.append(infoItem) + + # add INV3/INV5 tag + if vcfRec.isINV3: + newInfo.append("INV3") + elif vcfRec.isINV5: + newInfo.append("INV5") + + vcfRec.info = newInfo + + vcfRec.makeLine() + + # make sure the vcf is sorted in genomic order + if (not vcfRec.chr == bufferedChr) or (vcfRec.pos > bufferedPos): + if lineBuffer: + writeLines(lineBuffer) + + lineBuffer = [vcfRec.line] + bufferedChr = vcfRec.chr + bufferedPos = vcfRec.pos + elif vcfRec.pos < bufferedPos: + lineBuffer.insert(0, vcfRec.line) + else: + lineBuffer.append(vcfRec.line) + + if lineBuffer: + writeLines(lineBuffer) + + + +if __name__=='__main__': + + usage = "convertInversion.py \n" + if len(sys.argv) <= 3: + sys.stderr.write(usage) + sys.exit(1) + + samtools = sys.argv[1] + refFasta = sys.argv[2] + vcfFile = sys.argv[3] + + for inputFile in [samtools, refFasta, vcfFile]: + if not(exists(inputFile)): + errMsg = ('File %s does not exist.' + % inputFile) + sys.stderr.write(errMsg + '\nProgram exits.') + sys.exit(1) + + invMateDict = scanVcf(vcfFile) + convertInversions(samtools, refFasta, vcfFile, invMateDict) diff --git a/bin/id_annotation.py b/bin/id_annotation.py new file mode 100644 index 0000000..1289459 --- /dev/null +++ b/bin/id_annotation.py @@ -0,0 +1,52 @@ +import argparse +import pysam +import logging +import os +import subprocess as sp + +logging.basicConfig( + format='%(levelname)-7s | %(asctime)s | %(message)s', + datefmt='%H:%M:%S') +logger = logging.getLogger(__name__) +logger.setLevel(logging.INFO) + + +parser = argparse.ArgumentParser() +parser.add_argument('--vcf', required=True, help='Input VCF') +args = parser.parse_args() + + + +in_vcf = pysam.VariantFile(args.vcf) +out_name = os.path.basename(args.vcf) +if out_name.endswith('.gz'): + out_name = out_name[:-3] +if out_name.endswith('.vcf'): + out_name = out_name[:-4] + +anno_header = in_vcf.header + +anno_vcf = pysam.VariantFile('{}.annotated.vcf.gz'.format(out_name), 'w', header=anno_header) + +logger.info('Adding CHROM_POS_TYPE to ID') +for v in in_vcf: + svlen=(len(v.alts[0])-len(v.ref)) + if (svlen < 0): + string=str(v.chrom) +'_'+ str(v.pos) + '_DEL' + v.id =string + elif (svlen > 0): + string=str(v.chrom) +'_'+ str(v.pos) + '_INS' + v.id =string + else: + string=str(v.chrom) +'_'+ str(v.pos) + '_SNP' + v.id =string + + +in_vcf.close() +anno_vcf.close() +logger.info('Finished') + +sp.run(['tabix', anno_vcf.filename]) +logger.info('VCF indexed') + +logger.info('DONE') diff --git a/bin/parse_vcf.py b/bin/parse_vcf.py new file mode 100644 index 0000000..6444b1d --- /dev/null +++ b/bin/parse_vcf.py @@ -0,0 +1,50 @@ +import argparse +import pysam +import logging +import os +import subprocess as sp + +logging.basicConfig( + format='%(levelname)-7s | %(asctime)s | %(message)s', + datefmt='%H:%M:%S') +logger = logging.getLogger(__name__) +logger.setLevel(logging.INFO) + +parser = argparse.ArgumentParser() +parser.add_argument('--vcf', required=True, help='Input VCF') +args = parser.parse_args() + +in_vcf = pysam.VariantFile(args.vcf) +out_name = os.path.basename(args.vcf) +if out_name.endswith('.gz'): + out_name = out_name[:-3] +if out_name.endswith('.vcf'): + out_name = out_name[:-4] + +out_header = in_vcf.header +out_vcf = pysam.VariantFile('{}.resolved.vcf.gz'.format(out_name), 'w', header=out_header) + +logger.info('Only will be sequence resolved, BND, and are filtered out!') +for v in in_vcf: + if v.info['SVTYPE'] == 'BND': + continue + else: + if v.alts[0] == '': + continue + + elif v.alts[0] == '': + continue + + elif v.alts[0] == '': + v.alleles = (fasta_f.fetch(v.chrom, v.pos - 1, v.stop), v.ref) + out_vcf.write(v) + else: + out_vcf.write(v) +fasta_f.close() +in_vcf.close() +out_vcf.close() + +sp.run(['tabix', out_vcf.filename]) +logger.info('VCF indexed') + +logger.info('DONE') diff --git a/bin/simple_event_annotator.R b/bin/simple_event_annotator.R new file mode 100644 index 0000000..ec99571 --- /dev/null +++ b/bin/simple_event_annotator.R @@ -0,0 +1,59 @@ +#!/usr/bin/env Rscript +# +# Copyright GRIDSS +## original version : https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R + +#source("https://bioconductor.org/biocLite.R") +#biocLite("VariantAnnotation") +#install_github("PapenfussLab/StructuralVariantAnnotation") +#install.packages("stringr") +library(VariantAnnotation) +library(StructuralVariantAnnotation) +library(stringr) +library(getopt) +#' Simple SV type classifier +## USAGE +## simple_event_annotator.R infile outfile genome + + +simpleEventType <- function(gr) { + return(ifelse(seqnames(gr) != seqnames(partner(gr)), "ITX", # inter-chromosomosal + ifelse(gr$insLen >= abs(gr$svLen) * 0.7, "INS", # TODO: improve classification of complex events + ifelse(strand(gr) == strand(partner(gr)), "INV", + ifelse(xor(start(gr) < start(partner(gr)), strand(gr) == "-"), "DEL", + "DUP"))))) +} +cmdArgs = commandArgs(TRUE) +print(cmdArgs) +if (length(cmdArgs) < 3) print(paste("Incorrect number of arguments (3 expected): ",length(cmdArgs))) +infile = cmdArgs[1] +outfile = cmdArgs[2] +genome = cmdArgs[3] + + +# using the example in the GRIDSS /example directory +vcf <- readVcf(infile, genome) +gr <- breakpointRanges(vcf) +svtype <- simpleEventType(gr) +info(vcf)$SIMPLE_TYPE <- NA_character_ +info(vcf[gr$vcfId])$SIMPLE_TYPE <- svtype +info(vcf[gr$vcfId])$SVLEN <- gr$svLen +writeVcf(vcf, outfile) + +## TODO: perform event filtering here +## By default, GRIDSS is very sensitive but this comes at the cost of a high false discovery rate +#gr <- gr[gr$FILTER == "PASS" & partner(gr)$FILTER == "PASS"] # Remove low confidence calls + +#simplegr <- gr[simpleEventType(gr) %in% c("INS", "INV", "DEL", "DUP")] +#simplebed <- data.frame( +# chrom=seqnames(simplegr), +# # call the centre of the homology/inexact interval +# start=as.integer((start(simplegr) + end(simplegr)) / 2), +# end=as.integer((start(partner(simplegr)) + end(partner(simplegr))) / 2), +# name=simpleEventType(simplegr), +# score=simplegr$QUAL, +# strand="." +# ) +## Just the lower of the two breakends so we don't output everything twice +#simplebed <- simplebed[simplebed$start < simplebed$end,] +#write.table(simplebed, "chr12.1527326.DEL1024.simple.bed", quote=FALSE, sep='\t', row.names=FALSE, col.names=FALSE) \ No newline at end of file diff --git a/conf/base.config b/conf/base.config index 194b09b..a927e41 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/variantbenchmarking Nextflow base config file + nf-core/benchmark Nextflow base config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A 'blank slate' config file, appropriate for general use on most high performance compute environments. Assumes that all software is installed and available on @@ -28,17 +28,17 @@ process { // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { cpus = { check_max( 1 , 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } + memory = { check_max( 4.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } } withLabel:process_low { cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } + memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } } withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } + cpus = { check_max( 4 * task.attempt, 'cpus' ) } + memory = { check_max( 8.GB * task.attempt, 'memory' ) } time = { check_max( 8.h * task.attempt, 'time' ) } } withLabel:process_high { diff --git a/conf/igenomes.config b/conf/igenomes.config index 3f11437..cd55be5 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -13,6 +13,7 @@ params { genomes { 'GRCh37' { fasta = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa" + fai = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/WholeGenomeFasta/genome.fa.fai" bwa = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/BWAIndex/version0.6.0/" bowtie2 = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/Ensembl/GRCh37/Sequence/STARIndex/" @@ -26,6 +27,7 @@ params { } 'GRCh38' { fasta = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa" + fai = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/WholeGenomeFasta/genome.fa.fai" bwa = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/BWAIndex/version0.6.0/" bowtie2 = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/NCBI/GRCh38/Sequence/STARIndex/" @@ -38,249 +40,16 @@ params { } 'CHM13' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" + fai = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa.fai" bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/" bwamem2 = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/" gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf" gff = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz" mito_name = "chrM" } - 'GRCm38' { - fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/GRCm38-blacklist.bed" - } - 'TAIR10' { - fasta = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Arabidopsis_thaliana/Ensembl/TAIR10/Annotation/README.txt" - mito_name = "Mt" - } - 'EB2' { - fasta = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bacillus_subtilis_168/Ensembl/EB2/Annotation/README.txt" - } - 'UMD3.1' { - fasta = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Bos_taurus/Ensembl/UMD3.1/Annotation/README.txt" - mito_name = "MT" - } - 'WBcel235' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/Ensembl/WBcel235/Annotation/Genes/genes.bed" - mito_name = "MtDNA" - macs_gsize = "9e7" - } - 'CanFam3.1' { - fasta = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/Ensembl/CanFam3.1/Annotation/README.txt" - mito_name = "MT" - } - 'GRCz10' { - fasta = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/Ensembl/GRCz10/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'BDGP6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/Ensembl/BDGP6/Annotation/Genes/genes.bed" - mito_name = "M" - macs_gsize = "1.2e8" - } - 'EquCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/Ensembl/EquCab2/Annotation/README.txt" - mito_name = "MT" - } - 'EB1' { - fasta = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Escherichia_coli_K_12_DH10B/Ensembl/EB1/Annotation/README.txt" - } - 'Galgal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/Ensembl/Galgal4/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Gm01' { - fasta = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Glycine_max/Ensembl/Gm01/Annotation/README.txt" - } - 'Mmul_1' { - fasta = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Macaca_mulatta/Ensembl/Mmul_1/Annotation/README.txt" - mito_name = "MT" - } - 'IRGSP-1.0' { - fasta = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Oryza_sativa_japonica/Ensembl/IRGSP-1.0/Annotation/Genes/genes.bed" - mito_name = "Mt" - } - 'CHIMP2.1.4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/Ensembl/CHIMP2.1.4/Annotation/README.txt" - mito_name = "MT" - } - 'Rnor_5.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_5.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'Rnor_6.0' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/Ensembl/Rnor_6.0/Annotation/Genes/genes.bed" - mito_name = "MT" - } - 'R64-1-1' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Saccharomyces_cerevisiae/Ensembl/R64-1-1/Annotation/Genes/genes.bed" - mito_name = "MT" - macs_gsize = "1.2e7" - } - 'EF2' { - fasta = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Schizosaccharomyces_pombe/Ensembl/EF2/Annotation/README.txt" - mito_name = "MT" - macs_gsize = "1.21e7" - } - 'Sbi1' { - fasta = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sorghum_bicolor/Ensembl/Sbi1/Annotation/README.txt" - } - 'Sscrofa10.2' { - fasta = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/Ensembl/Sscrofa10.2/Annotation/README.txt" - mito_name = "MT" - } - 'AGPv3' { - fasta = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Zea_mays/Ensembl/AGPv3/Annotation/Genes/genes.bed" - mito_name = "Mt" - } 'hg38' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa" + fai = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/WholeGenomeFasta/genome.fa.fai" bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/BWAIndex/version0.6.0/" bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg38/Sequence/STARIndex/" @@ -293,6 +62,7 @@ params { } 'hg19' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa" + fai = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/WholeGenomeFasta/genome.fa.fai" bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/BWAIndex/version0.6.0/" bowtie2 = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/Bowtie2Index/" star = "${params.igenomes_base}/Homo_sapiens/UCSC/hg19/Sequence/STARIndex/" @@ -304,137 +74,5 @@ params { macs_gsize = "2.7e9" blacklist = "${projectDir}/assets/blacklists/hg19-blacklist.bed" } - 'mm10' { - fasta = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Mus_musculus/UCSC/mm10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.87e9" - blacklist = "${projectDir}/assets/blacklists/mm10-blacklist.bed" - } - 'bosTau8' { - fasta = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Bos_taurus/UCSC/bosTau8/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'ce10' { - fasta = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Caenorhabditis_elegans/UCSC/ce10/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "9e7" - } - 'canFam3' { - fasta = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Canis_familiaris/UCSC/canFam3/Annotation/README.txt" - mito_name = "chrM" - } - 'danRer10' { - fasta = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Danio_rerio/UCSC/danRer10/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.37e9" - } - 'dm6' { - fasta = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Drosophila_melanogaster/UCSC/dm6/Annotation/Genes/genes.bed" - mito_name = "chrM" - macs_gsize = "1.2e8" - } - 'equCab2' { - fasta = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Equus_caballus/UCSC/equCab2/Annotation/README.txt" - mito_name = "chrM" - } - 'galGal4' { - fasta = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Gallus_gallus/UCSC/galGal4/Annotation/README.txt" - mito_name = "chrM" - } - 'panTro4' { - fasta = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Pan_troglodytes/UCSC/panTro4/Annotation/README.txt" - mito_name = "chrM" - } - 'rn6' { - fasta = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Rattus_norvegicus/UCSC/rn6/Annotation/Genes/genes.bed" - mito_name = "chrM" - } - 'sacCer3' { - fasta = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Sequence/BismarkIndex/" - readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" - mito_name = "chrM" - macs_gsize = "1.2e7" - } - 'susScr3' { - fasta = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/WholeGenomeFasta/genome.fa" - bwa = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BWAIndex/version0.6.0/" - bowtie2 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/Bowtie2Index/" - star = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/STARIndex/" - bismark = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Sequence/BismarkIndex/" - gtf = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.gtf" - bed12 = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/Genes/genes.bed" - readme = "${params.igenomes_base}/Sus_scrofa/UCSC/susScr3/Annotation/README.txt" - mito_name = "chrM" - } } } diff --git a/conf/modules.config b/conf/modules.config index d91c6ab..6e27229 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -26,10 +26,6 @@ process { ] } - withName: FASTQC { - ext.args = '--quiet' - } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, @@ -46,5 +42,181 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } - + withName: "BCFTOOLS_NORM_1" { + ext.args = {"--output-type z -N -m-any -c w" } + ext.prefix = {"${meta.id}.${meta2.caller}_norm"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/preprocess"}, + pattern: "*{.vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } + withName: "BCFTOOLS_NORM_2" { + ext.args = {"--output-type z --rm-du exact -c w" } + ext.prefix = {"${meta.id}.${meta2.caller}_dedup"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/preprocess"}, + pattern: "*{.vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } + withName: AWK_SORT { + ext.prefix = {"${meta.id}.${meta2.caller}_sort"} + publishDir = [ + path: { "${params.outdir}/test" }, + enabled: false + ] + } + withName: BCFTOOLS_RENAME_CHR { + ext.args = {"--output-type z" } + ext.prefix = {"${meta.id}.${meta2.caller}_rename_chr"} + publishDir = [ + path: { "${params.outdir}/test" }, + enabled: false + ] + } + withName: "BCFTOOLS_REHEADER_TRUTH" { + ext.args2 = {"--output-type v" } + ext.prefix = {"${meta.id}_truth"} + publishDir = [ + path: { "${params.outdir}/test" }, + enabled: false + ] + } + withName: "BCFTOOLS_REHEADER_TEST" { + ext.args2 = {"--output-type v" } + ext.prefix = {"${meta.id}_query"} + publishDir = [ + path: { "${params.outdir}/test" }, + enabled: false + ] + } + withName: BCFTOOLS_VIEW { + ext.args2 = {"--output-type z" } + ext.prefix = {"${meta.id}.${meta2.caller}_filter"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/"}, + pattern: "*{.vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } + withName: BCFTOOLS_ISEC { + ext.args = {"--output-type v" } + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/svanalyzer_bench/${meta2.caller}/isec"}, + mode: params.publish_dir_mode + ] + } + withName: SURVIVOR_STATS { + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/stats/survivor/"}, + pattern: "*{.stats}", + mode: params.publish_dir_mode + ] + } + withName: SURVIVOR_FILTER { + ext.prefix = {"${meta.id}.${meta2.caller}.filter"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/preprocess"}, + pattern: "*{.vcf}", + mode: params.publish_dir_mode + ] + } + withName: BCFTOOLS_STATS { + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/stats/bcftools/"}, + pattern: "*{stats.txt}", + mode: params.publish_dir_mode + ] + } + withName: "TRUVARI_PHAB" { + ext.prefix = {"${meta.id}.${meta2.caller}.harm"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/truvari_phab/${meta2.caller}"}, + pattern: "*{.vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } + withName: "TRUVARI_BENCH" { + ext.args = {"--pctsize 0.5 --pctovl 0.5 --refdist 1000"} + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/truvari_bench/${meta2.caller}"}, + pattern: "*{.vcf.gz,vcf.gz.tbi,json}", + mode: params.publish_dir_mode + ] + } + withName: SVANALYZER_SVBENCHMARK { + ext.args = {"-normshift 0.3 –normdist 0.3 –normsizediff 0.3"} + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/svanalyzer_bench/${meta2.caller}"}, + pattern: "*{.vcf,distances,log,report}", + mode: params.publish_dir_mode + ] + } + withName: WITTYER { + ext.args = {"--evaluationMode=CrossTypeAndSimpleCounting --percentDistance=0.3 --bpDistance=1000"} + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/wittyer_bench/${meta2.caller}"}, + pattern: "*{json,.vcf.gz.tbi,vcf.gz}", + mode: params.publish_dir_mode + ] + } + withName: VCFDIST { + ext.args = {"-v 0"} + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/vcfdist_bench/${meta2.caller}"}, + pattern: "*{.vcf,tsv}", + mode: params.publish_dir_mode + ] + } + withName: BAMSURGEON_EVALUATOR { + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/bamsurgeon_evalator/${meta2.caller}"}, + pattern: "*{.vcf}", + mode: params.publish_dir_mode + ] + } + withName: HAPPY_SOMPY { + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/sompy_bench/${meta2.caller}"}, + pattern: "*{.vcf.gz,vcf.gz.tbi,json,csv}", + mode: params.publish_dir_mode + ] + } + withName: MANTA_CONVERTINVERSION { + ext.prefix = {"${meta.id}.${meta2.caller}"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/preprocess/${meta2.caller}"}, + pattern: "*{.vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } + withName: SVYNC { + ext.prefix = {"${meta.id}.${meta2.caller}.stnd"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/preprocess"}, + pattern: "*{.vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } +} +// +// Don't publish results for these processes +// +process { + withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX' { + publishDir = [ + path: { "${params.outdir}/test" }, + enabled: false + ] + } } diff --git a/conf/test_data.config b/conf/test_data.config new file mode 100644 index 0000000..6a98dd0 --- /dev/null +++ b/conf/test_data.config @@ -0,0 +1,347 @@ +// README: +// https://github.com/nf-core/test-datasets/blob/modules/README.md + +params { + // Base directory for test data + test_data_base = "https://raw.githubusercontent.com/nf-core/test-datasets/modules" + + test_data { + 'homo_sapiens' { + 'genome' { + genome_elfasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.elfasta" + genome_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta" + genome_fasta_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.fai" + genome_fasta_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz" + genome_fasta_gz_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz.fai" + genome_fasta_gz_gzi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz.gzi" + genome_strtablefile = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_strtablefile.zip" + genome_dict = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.dict" + genome_gff3 = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.gff3" + genome_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.gtf" + genome_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.interval_list" + genome_multi_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.multi_intervals.bed" + genome_blacklist_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed" + genome_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.sizes" + genome_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed" + genome_header = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.header" + genome_bed_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed.gz" + genome_bed_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed.gz.tbi" + genome_elsites = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.elsites" + transcriptome_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/transcriptome.fasta" + genome2_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome2.fasta" + genome_chain_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.chain.gz" + genome_annotated_interval_tsv = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.annotated_intervals.tsv" + genome_mt_gb = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.NC_012920_1.gb" + genome_preprocessed_count_tsv = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.preprocessed_intervals.counts.tsv" + genome_preprocessed_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.preprocessed_intervals.interval_list" + genome_ploidy_model = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.ploidy_model.tar.gz" + genome_ploidy_calls = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.ploidy_calls.tar.gz" + genome_germline_cnv_model = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.germline_cnv_model.tar.gz" + genome_germline_cnv_calls = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.germline_cnv_calls.tar.gz" + genome_motifs = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_motifs.txt" + genome_config = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_config.json" + + genome_1_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr1/genome.fasta.gz" + genome_1_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr1/genome.gtf" + + genome_21_sdf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome_sdf.tar.gz" + genome_21_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta" + genome_21_fasta_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai" + genome_21_gencode_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chr21_gencode.gtf" + genome_21_dict = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.dict" + genome_21_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.sizes" + genome_21_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.interval_list" + genome_21_annotated_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/annotated.bed" + genome_21_multi_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed" + genome_21_multi_interval_antitarget_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.antitarget.bed" + genome_21_multi_interval_bed_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed.gz" + genome_21_multi_interval_bed_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed.gz.tbi" + genome_21_chromosomes_dir = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chromosomes.tar.gz" + genome_21_reference_cnn = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/reference_chr21.cnn" + genome_21_eigenstrat_snp = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chr_21.snp" + genome_21_stitch_posfile = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/dbsnp_138.hg38.first_10_biallelic_sites.tsv" + + dbsnp_146_hg38_elsites = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.elsites" + dbsnp_146_hg38_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz" + dbsnp_146_hg38_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi" + gnomad_r2_1_1_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz" + gnomad_r2_1_1_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz.tbi" + mills_and_1000g_indels_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz" + mills_and_1000g_indels_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi" + syntheticvcf_short_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.vcf.gz" + syntheticvcf_short_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.vcf.gz.tbi" + syntheticvcf_short_score = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.score" + gnomad_r2_1_1_sv_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1-sv.vcf.gz" + gnomad2_r2_1_1_sv_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD2.r2.1.1-sv.vcf.gz" + + hapmap_3_3_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/hapmap_3.3.hg38.vcf.gz" + hapmap_3_3_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/hapmap_3.3.hg38.vcf.gz.tbi" + res_1000g_omni2_5_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_omni2.5.hg38.vcf.gz" + res_1000g_omni2_5_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_omni2.5.hg38.vcf.gz.tbi" + res_1000g_phase1_snps_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_phase1.snps.hg38.vcf.gz" + res_1000g_phase1_snps_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_phase1.snps.hg38.vcf.gz.tbi" + dbsnp_138_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbsnp_138.hg38.vcf.gz" + dbsnp_138_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbsnp_138.hg38.vcf.gz.tbi" + gnomad_r2_1_1_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/gnomAD.r2.1.1.vcf.gz" + gnomad_r2_1_1_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/gnomAD.r2.1.1.vcf.gz.tbi" + mills_and_1000g_indels_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/mills_and_1000G.indels.hg38.vcf.gz" + mills_and_1000g_indels_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/mills_and_1000G.indels.hg38.vcf.gz.tbi" + haplotype_map = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/haplotype_map.txt" + dbNSFP_4_1a_21_hg38_txt_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbNSFP4.1a.21.txt.gz" + dbNSFP_4_1a_21_hg38_txt_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbNSFP4.1a.21.txt.gz.tbi" + ngscheckmate_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/SNP_GRCh38_hg38_wChr.bed" + + index_salmon = "${params.test_data_base}/data/genomics/homo_sapiens/genome/index/salmon" + repeat_expansions = "${params.test_data_base}/data/genomics/homo_sapiens/genome/loci/repeat_expansions.json" + justhusky_ped = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky.ped" + justhusky_minimal_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky_minimal.vcf.gz" + justhusky_minimal_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky_minimal.vcf.gz.tbi" + + vcfanno_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/vcfanno/vcfanno_grch38_module_test.tar.gz" + vcfanno_toml = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/vcfanno/vcfanno.toml" + updsites_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/updsites.bed" + + prg_input = "${params.test_data_base}/data/genomics/homo_sapiens/genome/PRG_test.zip" + crispr_functional_counts = "${params.test_data_base}/data/genomics/homo_sapiens/genome/tsv/functional_genomics_counts.tsv" + crispr_functional_library = "${params.test_data_base}/data/genomics/homo_sapiens/genome/tsv/library_functional_genomics.tsv" + + vep_cache = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vep.tar.gz" + affy_array_samplesheet = "${params.test_data_base}/data/genomics/homo_sapiens/array_expression/GSE38751.csv" + affy_array_celfiles_tar = "${params.test_data_base}/data/genomics/homo_sapiens/array_expression/GSE38751_RAW.tar" + + } + 'pangenome' { + pangenome_fa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa" + pangenome_fa_bgzip = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz" + pangenome_fa_bgzip_fai = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz.fai" + pangenome_fa_bgzip_gzi = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz.gzi" + pangenome_paf = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.paf" + pangenome_paf_gz = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.paf.gz" + pangenome_seqwish_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.seqwish.gfa" + pangenome_smoothxg_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.smoothxg.gfa" + pangenome_gfaffix_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.gfaffix.gfa" + 'odgi' { + pangenome_og = "${params.test_data_base}/data/pangenomics/homo_sapiens/odgi/pangenome.og" + pangenome_lay = "${params.test_data_base}/data/pangenomics/homo_sapiens/odgi/pangenome.lay" + } + } + 'illumina' { + test_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam" + test_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai" + test_paired_end_name_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.name.sorted.bam" + test_paired_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam" + test_paired_end_markduplicates_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai" + test_paired_end_markduplicates_sorted_referencesn_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.referencesn.txt" + test_paired_end_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam" + test_paired_end_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam.bai" + test_paired_end_umi_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_consensus.bam" + test_paired_end_umi_converted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_converted.bam" + test_paired_end_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_grouped.bam" + test_paired_end_umi_histogram_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_histogram.txt" + test_paired_end_umi_unsorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_unsorted.bam" + test_paired_end_umi_unsorted_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.unsorted_tagged.bam" + test_paired_end_hla = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.bam" + test_paired_end_hla_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam" + test_paired_end_hla_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam.bai" + test_rna_paired_end_sorted_chr6_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.chr6.bam" + test_rna_paired_end_sorted_chr6_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.chr6.bam.bai" + + test2_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam" + test2_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam.bai" + test2_paired_end_name_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.name.sorted.bam" + test2_paired_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.markduplicates.sorted.bam" + test2_paired_end_markduplicates_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.markduplicates.sorted.bam.bai" + test2_paired_end_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam" + test2_paired_end_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam.bai" + test2_paired_end_umi_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_consensus.bam" + test2_paired_end_umi_converted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_converted.bam" + test2_paired_end_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_grouped.bam" + test2_paired_end_umi_histogram_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_histogram.txt" + test2_paired_end_umi_unsorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_unsorted.bam" + test2_paired_end_umi_unsorted_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.unsorted_tagged.bam" + test_paired_end_duplex_umi_unmapped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_unmapped.bam" + test_paired_end_duplex_umi_mapped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_mapped.bam" + test_paired_end_duplex_umi_mapped_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_mapped_tagged.bam" + test_paired_end_duplex_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_grouped.bam" + test_paired_end_duplex_umi_duplex_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_duplex_consensus.bam" + + mitochon_standin_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam" + mitochon_standin_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam.bai" + test_illumina_mt_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test_illumina_mt.bam" + test_illumina_mt_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test_illumina_mt.bam.bai" + + test3_single_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam" + + read_group_settings_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/read_group_settings.txt" + + test_paired_end_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram" + test_paired_end_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai" + test_paired_end_markduplicates_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.markduplicates.sorted.cram" + test_paired_end_markduplicates_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.markduplicates.sorted.cram.crai" + test_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram" + test_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai" + + test2_paired_end_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.sorted.cram" + test2_paired_end_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.sorted.cram.crai" + test2_paired_end_markduplicates_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.markduplicates.sorted.cram" + test2_paired_end_markduplicates_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.markduplicates.sorted.cram.crai" + test2_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram" + test2_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram.crai" + test3_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test3.paired_end.recalibrated.sorted.cram" + test3_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test3.paired_end.recalibrated.sorted.cram.crai" + + test_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz" + test_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz" + test_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test.umi_1.fastq.gz" + test_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test.umi_2.fastq.gz" + test2_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz" + test2_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2_2.fastq.gz" + test2_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2.umi_1.fastq.gz" + test2_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2.umi_2.fastq.gz" + test_rnaseq_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz" + test_rnaseq_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz" + test_paired_end_duplex_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_duplex_umi_1.fastq.gz" + test_paired_end_duplex_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_duplex_umi_2.fastq.gz" + + test_baserecalibrator_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test.baserecalibrator.table" + test2_baserecalibrator_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test2.baserecalibrator.table" + test_pileups_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test.pileups.table" + test2_pileups_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test2.pileups.table" + + test_paired_end_sorted_dragstrmodel = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_paired_end_sorted_dragstrmodel.txt" + + test_genomicsdb_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_genomicsdb.tar.gz" + test_pon_genomicsdb_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_pon_genomicsdb.tar.gz" + + test2_haplotc_ann_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz" + test2_haplotc_ann_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz.tbi" + test_haplotc_cnn_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test_haplotcaller.cnn.vcf.gz" + test_haplotc_cnn_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test_haplotcaller.cnn.vcf.gz.tbi" + + test2_haplotc_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz" + test2_haplotc_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz.tbi" + + test2_recal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal" + test2_recal_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal.idx" + test2_tranches = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.tranches" + test2_allele_specific_recal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.recal" + test2_allele_specific_recal_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.recal.idx" + test2_allele_specific_tranches = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.tranches" + + test_test2_paired_mutect2_calls_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz" + test_test2_paired_mutect2_calls_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz.tbi" + test_test2_paired_mutect2_calls_vcf_gz_stats = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz.stats" + test_test2_paired_mutect2_calls_f1r2_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.f1r2.tar.gz" + test_test2_paired_mutect2_calls_artifact_prior_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired_mutect2_calls.artifact-prior.tar.gz" + test_test2_paired_segmentation_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired.segmentation.table" + test_test2_paired_contamination_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired.contamination.table" + + test_genome_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf" + test_genome_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.gz" + test_genome_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.gz.tbi" + test_genome_vcf_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.idx" + + test_genome_vcf_ud = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.UD" + test_genome_vcf_mu = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.mu" + test_genome_vcf_bed = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.bed" + + test2_genome_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf" + test2_genome_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.gz" + test2_genome_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.gz.tbi" + test2_genome_vcf_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.idx" + + test_genome21_indels_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.genome_21.somatic_sv.vcf.gz" + test_genome21_indels_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.genome_21.somatic_sv.vcf.gz.tbi" + + test_mpileup = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/mpileup/test.mpileup.gz" + test2_mpileup = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/mpileup/test2.mpileup.gz" + + test_broadpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/broadpeak/test.broadPeak" + test2_broadpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/broadpeak/test2.broadPeak" + + test_narrowpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/narrowpeak/test.narrowPeak" + test2_narrowpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/narrowpeak/test2.narrowPeak" + + test_yak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/yak/test.yak" + test2_yak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/yak/test2.yak" + + cutandrun_bedgraph_test_1 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/cutandtag_h3k27me3_test_1.bedGraph" + cutandrun_bedgraph_test_2 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/cutandtag_igg_test_1.bedGraph" + na24385_chr22_coverage = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/NA24385_coverage.bed" + + empty_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/empty.vcf.gz" + empty_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/empty.vcf.gz.tbi" + + simulated_sv = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv.vcf.gz" + simulated_sv_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv.vcf.gz.tbi" + simulated_sv2 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv2.vcf.gz" + simulated_sv2_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv2.vcf.gz.tbi" + + test_rnaseq_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.rnaseq.vcf" + test_sv_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz" + test_sv_vcf_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz.tbi" + na24385_chr22_sv_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" + na24385_chr22_sv_vcf_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz.tbi" + genmod_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/genmod.vcf.gz" + genmod_annotate_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_annotate.vcf.gz" + genmod_models_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_models.vcf.gz" + genmod_score_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_score.vcf.gz" + + test_mito_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA12878_chrM.vcf.gz" + + test_pytor = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/pytor/test.pytor" + rank_model = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/genmod/svrank_model_-v1.8-.ini" + + test_flowcell = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bcl/flowcell.tar.gz" + test_flowcell_samplesheet = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bcl/flowcell_samplesheet.csv" + + varlociraptor_scenario = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/varlociraptor/scenario.yml" + + contig_ploidy_priors_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/contig_ploidy_priors_table.tsv" + + purecn_ex1_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1.bam" + purecn_ex1_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1.bam.bai" + purecn_ex1_interval = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1_intervals.txt" + purecn_ex1_normal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1_normal.txt.gz" + purecn_ex2_normal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex2_normal.txt.gz" + purecn_normalpanel_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_normalpanel.vcf.gz" + purecn_normalpanel_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_normalpanel.vcf.gz.tbi" + } + 'gene_set_analysis' { + gct = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/P53_6samples_collapsed_symbols.gct" + cls = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/P53_6samples.cls" + gmx = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/c1.symbols.reduced.gmx" + } + 'cnvkit' { + amplicon_cnr = "https://raw.githubusercontent.com/etal/cnvkit/v0.9.9/test/formats/amplicon.cnr" + amplicon_cns = "https://raw.githubusercontent.com/etal/cnvkit/v0.9.9/test/formats/amplicon.cns" + } + } + 'generic' { + 'csv' { + test_csv = "${params.test_data_base}/data/generic/csv/test.csv" + } + 'notebooks' { + rmarkdown = "${params.test_data_base}/data/generic/notebooks/rmarkdown/rmarkdown_notebook.Rmd" + ipython_md = "${params.test_data_base}/data/generic/notebooks/jupyter/ipython_notebook.md" + ipython_ipynb = "${params.test_data_base}/data/generic/notebooks/jupyter/ipython_notebook.ipynb" + } + 'tar' { + tar_gz = "${params.test_data_base}/data/generic/tar/hello.tar.gz" + } + 'tsv' { + test_tsv = "${params.test_data_base}/data/generic/tsv/test.tsv" + } + 'txt' { + hello = "${params.test_data_base}/data/generic/txt/hello.txt" + } + 'unsorted_data' { + 'unsorted_text' { + genome_file = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.genome" + intervals = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.bed" + numbers_csv = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.csv" + } + } + } + } +} diff --git a/conf/test_full.config b/conf/test_full.config index 57fba2a..7693ac0 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_full, --outdir + nextflow run nf-core/benchmark -profile test_full, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_hg19.config b/conf/test_hg19.config new file mode 100644 index 0000000..2734e5a --- /dev/null +++ b/conf/test_hg19.config @@ -0,0 +1,42 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/benchmark -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 16 + max_memory = 100.GB + max_time = '8.h' + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'assets/samplesheet_HG002.csv' + outdir = 'results' + + // Genome references + genome = 'GRCh37' + analysis = 'germline' //somatic + method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist + + similarity = 0 // determines the sequence similarity level in benchmarking. + standardization = true + preprocess = "normalization, deduplication" + //bnd_to_inv = true + + sample = "HG002" // available samples: SEQC2, HG002 + truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.vcf.gz" + high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.bed" + +} diff --git a/conf/test_hg37.config b/conf/test_hg37.config new file mode 100644 index 0000000..97db262 --- /dev/null +++ b/conf/test_hg37.config @@ -0,0 +1,43 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/benchmark -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 16 + max_memory = 100.GB + max_time = '8.h' + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'assets/samplesheet_HG002.csv' + outdir = 'results' + + // Genome references + genome = 'GRCh37' + analysis = 'germline' //somatic + method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist + + similarity = 0 // determines the sequence similarity level in benchmarking. + standardization = true + preprocess = "normalization, deduplication" + //bnd_to_inv = true + + sample = "HG002" // available samples: SEQC2, HG002 + truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.vcf.gz" + high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.bed" + +} + diff --git a/conf/test_hg38.config b/conf/test_hg38.config new file mode 100644 index 0000000..05958d2 --- /dev/null +++ b/conf/test_hg38.config @@ -0,0 +1,53 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/benchmark -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 16 + max_memory = '100.GB' + max_time = '8.h' + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'assets/samplesheet_HG002_hg38.csv' + outdir = 'results' + + // Genome references + genome = 'hg38' + + // Processes + analysis = 'germline' //somatic + method = 'truvari,svanalyzer,wittyer,vcfdist' // --not working for now : wittyer, vcfdist + //harmonize = true // ATTENTION : takes long time because of multi-alignment process. + similarity = 0 // determines the sequence similarity level in benchmarking. + preprocess = "normalization, deduplication" + + // Truvari params + //dup_to_ins = true // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 + + // Manta params + //bnd_to_inv = true // manta reports INV as BND + + // Gridss params + //gridss_annotate = true + + //standardization = true + + sample = "HG002" // available samples: SEQC2, HG002 + truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.vcf.gz" + high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.bed" + +} diff --git a/conf/test_hg38_somatic.config b/conf/test_hg38_somatic.config new file mode 100644 index 0000000..23e6ecb --- /dev/null +++ b/conf/test_hg38_somatic.config @@ -0,0 +1,39 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/benchmark -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 16 + max_memory = '100.GB' + max_time = '8.h' + + // Input data + // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets + // TODO nf-core: Give any required params for the test so that command line flags are not needed + input = 'assets/samplesheet_SEQC2.csv' + outdir = 'results' + + // Genome references + genome = 'hg38' + analysis = 'somatic' //somatic + method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist + + + sample = "SEQC2" // available samples: SEQC2, HG002 + truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/sSNV_truth_set_v1.0.vcf.gz" + high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/High-Confidence_Regions_v1.2.bed" + rename_chromosomes = "assets/rename_chroms_hg38.txt" + +} diff --git a/conf/truth.config b/conf/truth.config new file mode 100644 index 0000000..0a89ef4 --- /dev/null +++ b/conf/truth.config @@ -0,0 +1,24 @@ + +params { + // Base directory for test data + truth { + 'GRCh38' { + 'germline' { + 'HG002'{ + sv_bed = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/NIST_GIAB/HG002_SVs_Tier1_v0.6.bed" + snv_bed = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/NIST_GIAB/HG002_GRCh38_1_22_v4.2.1_benchmark_noinconsistent.bed" + } + } + 'somatic' { + 'HG002'{ + sv_bed = '/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_CMRG_SV_v1.00.bed' + snv_bed = '/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_CMRG_smallvar_v1.00.bed' + } + 'SEQC2'{ + snv_bed = '/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/High-Confidence_Regions_v1.2.bed' + sv_bed = '/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/High-Confidence_Regions_v1.2.bed' + } + } + } + } +} diff --git a/docs/README.md b/docs/README.md index c79ccc9..23c4836 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,6 +1,6 @@ -# nf-core/variantbenchmarking: Documentation +# nf-core/benchmark: Documentation -The nf-core/variantbenchmarking documentation is split into the following pages: +The nf-core/benchmark documentation is split into the following pages: - [Usage](usage.md) - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. diff --git a/docs/output.md b/docs/output.md index c671e3f..34599a9 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,4 +1,4 @@ -# nf-core/variantbenchmarking: Output +# nf-core/benchmark: Output ## Introduction diff --git a/docs/usage.md b/docs/usage.md index 3093473..365ca0c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,6 +1,6 @@ -# nf-core/variantbenchmarking: Usage +# nf-core/benchmark: Usage -## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/variantbenchmarking/usage](https://nf-co.re/variantbenchmarking/usage) +## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/benchmark/usage](https://nf-co.re/benchmark/usage) > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ @@ -57,7 +57,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/variantbenchmarking --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-core/benchmark --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -82,7 +82,7 @@ Do not use `-c ` to specify parameters as this will result in errors. Cust The above pipeline run specified with a params file in yaml format: ```bash -nextflow run nf-core/variantbenchmarking -profile docker -params-file params.yaml +nextflow run nf-core/benchmark -profile docker -params-file params.yaml ``` with `params.yaml` containing: @@ -101,14 +101,14 @@ You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-c When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash -nextflow pull nf-core/variantbenchmarking +nextflow pull nf-core/benchmark ``` ### Reproducibility It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [nf-core/variantbenchmarking releases page](https://github.com/nf-core/variantbenchmarking/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. +First, go to the [nf-core/benchmark releases page](https://github.com/nf-core/benchmark/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy new file mode 100644 index 0000000..33cd4f6 --- /dev/null +++ b/lib/NfcoreSchema.groovy @@ -0,0 +1,528 @@ +// +// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. +// + +import org.everit.json.schema.Schema +import org.everit.json.schema.loader.SchemaLoader +import org.everit.json.schema.ValidationException +import org.json.JSONObject +import org.json.JSONTokener +import org.json.JSONArray +import groovy.json.JsonSlurper +import groovy.json.JsonBuilder + +class NfcoreSchema { + + // + // Resolve Schema path relative to main workflow directory + // + public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { + return "${workflow.projectDir}/${schema_filename}" + } + + // + // Function to loop over all parameters defined in schema and check + // whether the given parameters adhere to the specifications + // + /* groovylint-disable-next-line UnusedPrivateMethodParameter */ + public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { + def has_error = false + //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// + // Check for nextflow core params and unexpected params + def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text + def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') + def nf_params = [ + // Options for base `nextflow` command + 'bg', + 'c', + 'C', + 'config', + 'd', + 'D', + 'dockerize', + 'h', + 'log', + 'q', + 'quiet', + 'syslog', + 'v', + + // Options for `nextflow run` command + 'ansi', + 'ansi-log', + 'bg', + 'bucket-dir', + 'c', + 'cache', + 'config', + 'dsl2', + 'dump-channels', + 'dump-hashes', + 'E', + 'entry', + 'latest', + 'lib', + 'main-script', + 'N', + 'name', + 'offline', + 'params-file', + 'pi', + 'plugins', + 'poll-interval', + 'pool-size', + 'profile', + 'ps', + 'qs', + 'queue-size', + 'r', + 'resume', + 'revision', + 'stdin', + 'stub', + 'stub-run', + 'test', + 'w', + 'with-charliecloud', + 'with-conda', + 'with-dag', + 'with-docker', + 'with-mpi', + 'with-notification', + 'with-podman', + 'with-report', + 'with-singularity', + 'with-timeline', + 'with-tower', + 'with-trace', + 'with-weblog', + 'without-docker', + 'without-podman', + 'work-dir' + ] + def unexpectedParams = [] + + // Collect expected parameters from the schema + def expectedParams = [] + def enums = [:] + for (group in schemaParams) { + for (p in group.value['properties']) { + expectedParams.push(p.key) + if (group.value['properties'][p.key].containsKey('enum')) { + enums[p.key] = group.value['properties'][p.key]['enum'] + } + } + } + + for (specifiedParam in params.keySet()) { + // nextflow params + if (nf_params.contains(specifiedParam)) { + log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" + has_error = true + } + // unexpected params + def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' + def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } + def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() + def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) + if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { + // Temporarily remove camelCase/camel-case params #1035 + def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} + if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ + unexpectedParams.push(specifiedParam) + } + } + } + + //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// + // Validate parameters against the schema + InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() + JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) + + // Remove anything that's in params.schema_ignore_params + raw_schema = removeIgnoredParams(raw_schema, params) + + Schema schema = SchemaLoader.load(raw_schema) + + // Clean the parameters + def cleanedParams = cleanParameters(params) + + // Convert to JSONObject + def jsonParams = new JsonBuilder(cleanedParams) + JSONObject params_json = new JSONObject(jsonParams.toString()) + + // Validate + try { + schema.validate(params_json) + } catch (ValidationException e) { + println '' + log.error 'ERROR: Validation of pipeline parameters failed!' + JSONObject exceptionJSON = e.toJSON() + printExceptions(exceptionJSON, params_json, log, enums) + println '' + has_error = true + } + + // Check for unexpected parameters + if (unexpectedParams.size() > 0) { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) + println '' + def warn_msg = 'Found unexpected parameters:' + for (unexpectedParam in unexpectedParams) { + warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" + } + log.warn warn_msg + log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" + println '' + } + + if (has_error) { + System.exit(1) + } + } + + // + // Beautify parameters for --help + // + public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) + Integer num_hidden = 0 + String output = '' + output += 'Typical pipeline command:\n\n' + output += " ${colors.cyan}${command}${colors.reset}\n\n" + Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) + Integer max_chars = paramsMaxChars(params_map) + 1 + Integer desc_indent = max_chars + 14 + Integer dec_linewidth = 160 - desc_indent + for (group in params_map.keySet()) { + Integer num_params = 0 + String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (group_params.get(param).hidden && !params.show_hidden_params) { + num_hidden += 1 + continue; + } + def type = '[' + group_params.get(param).type + ']' + def description = group_params.get(param).description + def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : '' + def description_default = description + colors.dim + defaultValue + colors.reset + // Wrap long description texts + // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap + if (description_default.length() > dec_linewidth){ + List olines = [] + String oline = "" // " " * indent + description_default.split(" ").each() { wrd -> + if ((oline.size() + wrd.size()) <= dec_linewidth) { + oline += wrd + " " + } else { + olines += oline + oline = wrd + " " + } + } + olines += oline + description_default = olines.join("\n" + " " * desc_indent) + } + group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' + num_params += 1 + } + group_output += '\n' + if (num_params > 0){ + output += group_output + } + } + if (num_hidden > 0){ + output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset + } + output += NfcoreTemplate.dashedLine(params.monochrome_logs) + return output + } + + // + // Groovy Map summarising parameters/workflow options used by the pipeline + // + public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { + // Get a selection of core Nextflow workflow options + def Map workflow_summary = [:] + if (workflow.revision) { + workflow_summary['revision'] = workflow.revision + } + workflow_summary['runName'] = workflow.runName + if (workflow.containerEngine) { + workflow_summary['containerEngine'] = workflow.containerEngine + } + if (workflow.container) { + workflow_summary['container'] = workflow.container + } + workflow_summary['launchDir'] = workflow.launchDir + workflow_summary['workDir'] = workflow.workDir + workflow_summary['projectDir'] = workflow.projectDir + workflow_summary['userName'] = workflow.userName + workflow_summary['profile'] = workflow.profile + workflow_summary['configFiles'] = workflow.configFiles.join(', ') + + // Get pipeline parameters defined in JSON Schema + def Map params_summary = [:] + def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) + for (group in params_map.keySet()) { + def sub_params = new LinkedHashMap() + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (params.containsKey(param)) { + def params_value = params.get(param) + def schema_value = group_params.get(param).default + def param_type = group_params.get(param).type + if (schema_value != null) { + if (param_type == 'string') { + if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { + def sub_string = schema_value.replace('\$projectDir', '') + sub_string = sub_string.replace('\${projectDir}', '') + if (params_value.contains(sub_string)) { + schema_value = params_value + } + } + if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { + def sub_string = schema_value.replace('\$params.outdir', '') + sub_string = sub_string.replace('\${params.outdir}', '') + if ("${params.outdir}${sub_string}" == params_value) { + schema_value = params_value + } + } + } + } + + // We have a default in the schema, and this isn't it + if (schema_value != null && params_value != schema_value) { + sub_params.put(param, params_value) + } + // No default in the schema, and this isn't empty + else if (schema_value == null && params_value != "" && params_value != null && params_value != false) { + sub_params.put(param, params_value) + } + } + } + params_summary.put(group, sub_params) + } + return [ 'Core Nextflow options' : workflow_summary ] << params_summary + } + + // + // Beautify parameters for summary and return as string + // + public static String paramsSummaryLog(workflow, params) { + Map colors = NfcoreTemplate.logColours(params.monochrome_logs) + String output = '' + def params_map = paramsSummaryMap(workflow, params) + def max_chars = paramsMaxChars(params_map) + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + if (group_params) { + output += colors.bold + group + colors.reset + '\n' + for (param in group_params.keySet()) { + output += " " + colors.blue + param.padRight(max_chars) + ": " + colors.green + group_params.get(param) + colors.reset + '\n' + } + output += '\n' + } + } + output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" + output += NfcoreTemplate.dashedLine(params.monochrome_logs) + return output + } + + // + // Loop over nested exceptions and print the causingException + // + private static void printExceptions(ex_json, params_json, log, enums, limit=5) { + def causingExceptions = ex_json['causingExceptions'] + if (causingExceptions.length() == 0) { + def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ + // Missing required param + if (m.matches()) { + log.error "* Missing required parameter: --${m[0][1]}" + } + // Other base-level error + else if (ex_json['pointerToViolation'] == '#') { + log.error "* ${ex_json['message']}" + } + // Error with specific param + else { + def param = ex_json['pointerToViolation'] - ~/^#\// + def param_val = params_json[param].toString() + if (enums.containsKey(param)) { + def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices" + if (enums[param].size() > limit) { + log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )" + } else { + log.error "${error_msg}: ${enums[param].join(', ')})" + } + } else { + log.error "* --${param}: ${ex_json['message']} (${param_val})" + } + } + } + for (ex in causingExceptions) { + printExceptions(ex, params_json, log, enums) + } + } + + // + // Remove an element from a JSONArray + // + private static JSONArray removeElement(json_array, element) { + def list = [] + int len = json_array.length() + for (int i=0;i + if(raw_schema.keySet().contains('definitions')){ + raw_schema.definitions.each { definition -> + for (key in definition.keySet()){ + if (definition[key].get("properties").keySet().contains(ignore_param)){ + // Remove the param to ignore + definition[key].get("properties").remove(ignore_param) + // If the param was required, change this + if (definition[key].has("required")) { + def cleaned_required = removeElement(definition[key].required, ignore_param) + definition[key].put("required", cleaned_required) + } + } + } + } + } + if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { + raw_schema.get("properties").remove(ignore_param) + } + if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { + def cleaned_required = removeElement(raw_schema.required, ignore_param) + raw_schema.put("required", cleaned_required) + } + } + return raw_schema + } + + // + // Clean and check parameters relative to Nextflow native classes + // + private static Map cleanParameters(params) { + def new_params = params.getClass().newInstance(params) + for (p in params) { + // remove anything evaluating to false + if (!p['value']) { + new_params.remove(p.key) + } + // Cast MemoryUnit to String + if (p['value'].getClass() == nextflow.util.MemoryUnit) { + new_params.replace(p.key, p['value'].toString()) + } + // Cast Duration to String + if (p['value'].getClass() == nextflow.util.Duration) { + new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) + } + // Cast LinkedHashMap to String + if (p['value'].getClass() == LinkedHashMap) { + new_params.replace(p.key, p['value'].toString()) + } + } + return new_params + } + + // + // This function tries to read a JSON params file + // + private static LinkedHashMap paramsLoad(String json_schema) { + def params_map = new LinkedHashMap() + try { + params_map = paramsRead(json_schema) + } catch (Exception e) { + println "Could not read parameters settings from JSON. $e" + params_map = new LinkedHashMap() + } + return params_map + } + + // + // Method to actually read in JSON file using Groovy. + // Group (as Key), values are all parameters + // - Parameter1 as Key, Description as Value + // - Parameter2 as Key, Description as Value + // .... + // Group + // - + private static LinkedHashMap paramsRead(String json_schema) throws Exception { + def json = new File(json_schema).text + def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') + def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') + /* Tree looks like this in nf-core schema + * definitions <- this is what the first get('definitions') gets us + group 1 + title + description + properties + parameter 1 + type + description + parameter 2 + type + description + group 2 + title + description + properties + parameter 1 + type + description + * properties <- parameters can also be ungrouped, outside of definitions + parameter 1 + type + description + */ + + // Grouped params + def params_map = new LinkedHashMap() + schema_definitions.each { key, val -> + def Map group = schema_definitions."$key".properties // Gets the property object of the group + def title = schema_definitions."$key".title + def sub_params = new LinkedHashMap() + group.each { innerkey, value -> + sub_params.put(innerkey, value) + } + params_map.put(title, sub_params) + } + + // Ungrouped params + def ungrouped_params = new LinkedHashMap() + schema_properties.each { innerkey, value -> + ungrouped_params.put(innerkey, value) + } + params_map.put("Other parameters", ungrouped_params) + + return params_map + } + + // + // Get maximum number of characters across all parameter names + // + private static Integer paramsMaxChars(params_map) { + Integer max_chars = 0 + for (group in params_map.keySet()) { + def group_params = params_map.get(group) // This gets the parameters of that particular group + for (param in group_params.keySet()) { + if (param.size() > max_chars) { + max_chars = param.size() + } + } + } + return max_chars + } +} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 025eed8..d13ecad 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -1,5 +1,5 @@ // -// This file holds several functions specific to the main.nf workflow in the nf-core/variantbenchmarking pipeline +// This file holds several functions specific to the main.nf workflow in the nf-core/benchmark pipeline // import nextflow.Nextflow @@ -24,7 +24,7 @@ class WorkflowMain { // // Validate parameters and print summary to screen // - public static void initialise(workflow, params, log, args) { + public static void initialise(workflow, params, log) { // Print workflow version and exit on --version if (params.version) { @@ -35,8 +35,6 @@ class WorkflowMain { // Check that a -profile or Nextflow config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) // Check that conda channels are set-up correctly if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { @@ -62,16 +60,4 @@ class WorkflowMain { } return null } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } } diff --git a/lib/WorkflowVariantbenchmarking.groovy b/lib/WorkflowVariantbenchmarking.groovy old mode 100755 new mode 100644 index ae9238e..9f0a479 --- a/lib/WorkflowVariantbenchmarking.groovy +++ b/lib/WorkflowVariantbenchmarking.groovy @@ -1,11 +1,11 @@ // -// This file holds several functions specific to the workflow/variantbenchmarking.nf in the nf-core/variantbenchmarking pipeline +// This file holds several functions specific to the workflow/svbench.nf in the nf-core/svbench pipeline // import nextflow.Nextflow import groovy.text.SimpleTemplateEngine -class WorkflowVariantbenchmarking { +class WorkflowBenchmark { // // Check and validate parameters diff --git a/main.nf b/main.nf index 175c581..27d248d 100644 --- a/main.nf +++ b/main.nf @@ -1,11 +1,11 @@ #!/usr/bin/env nextflow /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/variantbenchmarking + nf-core/svbench ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Github : https://github.com/nf-core/variantbenchmarking - Website: https://nf-co.re/variantbenchmarking - Slack : https://nfcore.slack.com/channels/variantbenchmarking + Github : https://github.com/nf-core/svbench + Website: https://nf-co.re/svbench + Slack : https://nfcore.slack.com/channels/svbench ---------------------------------------------------------------------------------------- */ @@ -21,6 +21,7 @@ nextflow.enable.dsl = 2 // This is an example of how to use getGenomeAttribute() to fetch parameters // from igenomes.config using `--genome` params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +params.fai = WorkflowMain.getGenomeAttribute(params, 'fai') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -44,7 +45,7 @@ if (params.validate_params) { validateParameters() } -WorkflowMain.initialise(workflow, params, log, args) +WorkflowMain.initialise(workflow, params, log) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -52,13 +53,13 @@ WorkflowMain.initialise(workflow, params, log, args) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { VARIANTBENCHMARKING } from './workflows/variantbenchmarking' +include { SVBENCH } from './workflows/svbench' // -// WORKFLOW: Run main nf-core/variantbenchmarking analysis pipeline +// WORKFLOW: Run main nf-core/svbench analysis pipeline // -workflow NFCORE_VARIANTBENCHMARKING { - VARIANTBENCHMARKING () +workflow NFCORE_SVBENCH { + SVBENCH () } /* @@ -72,7 +73,7 @@ workflow NFCORE_VARIANTBENCHMARKING { // See: https://github.com/nf-core/rnaseq/issues/619 // workflow { - NFCORE_VARIANTBENCHMARKING () + NFCORE_SVBENCH () } /* diff --git a/modules.json b/modules.json index 789c505..881ab96 100644 --- a/modules.json +++ b/modules.json @@ -1,27 +1,62 @@ { - "name": "nf-core/variantbenchmarking", - "homePage": "https://github.com/nf-core/variantbenchmarking", - "repos": { - "https://github.com/nf-core/modules.git": { - "modules": { - "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, - "fastqc": { - "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", - "installed_by": ["modules"] - }, - "multiqc": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - } - } - } + "name": "nf-core/svbench", + "homePage": "https://github.com/nf-core/svbench", + "repos": { + "https://github.com/nf-core/modules.git": { + "modules": { + "nf-core": { + "bcftools/annotate": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "bcftools/norm": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "custom/dumpsoftwareversions": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "fastqc": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "happy/happy": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "happy/sompy": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "multiqc": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "tabix/tabix": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "truvari/bench": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "survivor/stats": { + "branch": "master", + "git_sha": "00d03755abd2cac54a039525716aeabea9efb9e7", + "installed_by": ["modules"] + } } + } } + } } diff --git a/modules/local/addhead.nf b/modules/local/addhead.nf new file mode 100644 index 0000000..89a2a50 --- /dev/null +++ b/modules/local/addhead.nf @@ -0,0 +1,38 @@ +process ADDHEAD { + tag "$meta.id $meta2.caller" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta), val(meta2), path(vcf), path(header) + + output: + tuple val(meta), val(meta2), path("*.vcf.gz") , emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + cat $header $vcf > ${prefix}_rehead.vcf + + bcftools \\ + sort \\ + --output ${prefix}.vcf.gz \\ + --output-type z \\ + --temp-dir . \\ + ${prefix}_rehead.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/local/awk_sort.nf b/modules/local/awk_sort.nf new file mode 100644 index 0000000..cb2dc29 --- /dev/null +++ b/modules/local/awk_sort.nf @@ -0,0 +1,50 @@ +process AWK_SORT { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': + 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" + + input: + tuple val(meta),val(meta2), path(vcf) + + output: + tuple val(meta),val(meta2), path("*.tmp.vcf.gz"),path("*.tmp.vcf.gz.tbi"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def zipname = vcf.getBaseName() + + if (vcf.getExtension() == "gz"){ + """ + bgzip -d $vcf + cat $zipname | awk '\$1 ~ /^#/ {print \$0;next} {print \$0 | "sort -k1,1 -k2,2n"}' > ${zipname}.tmp.vcf + bgzip -c ${zipname}.tmp.vcf > ${zipname}.tmp.vcf.gz + tabix -p vcf ${zipname}.tmp.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + } + else{ + """ + cat $vcf | awk '\$1 ~ /^#/ {print \$0;next} {print \$0 | "sort -k1,1 -k2,2n"}' > ${zipname}.tmp.vcf + bgzip -c ${zipname}.tmp.vcf > ${zipname}.tmp.vcf.gz + tabix -p vcf ${zipname}.tmp.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + } +} diff --git a/modules/local/bamsurgeon_evaluator.nf b/modules/local/bamsurgeon_evaluator.nf new file mode 100644 index 0000000..384a515 --- /dev/null +++ b/modules/local/bamsurgeon_evaluator.nf @@ -0,0 +1,42 @@ +process BAMSURGEON_EVALUATOR { + tag "$meta.id" + label 'process_low' + + conda "" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'docker://lethalfang/bamsurgeon:1.2': + 'lethalfang/bamsurgeon:1.2' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi) + tuple path(fasta), path(fai) + val(muttype) + + output: + tuple val(meta),val(meta2), path("*.{vcf}"), emit: bench + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + python3 /usr/local/bamsurgeon/scripts/evaluator.py \\ + -v $vcf \\ + -t $truth_vcf \\ + -f $fasta \\ + -m $muttype \\ + $args \\ + --fp ${prefix}.falsepositives.vcf \\ + --tp ${prefix}.truepositives.vcf \\ + --fn ${prefix}.falsenegatives.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bamsurgeon: v1.2 + END_VERSIONS + """ +} diff --git a/modules/local/bcftools_view.nf b/modules/local/bcftools_view.nf new file mode 100644 index 0000000..eab11f7 --- /dev/null +++ b/modules/local/bcftools_view.nf @@ -0,0 +1,48 @@ +process BCFTOOLS_VIEW { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(index) + + + output: + tuple val(meta),val(meta2), path("*.vcf") , emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + def regions = params.genome.contains("38")? "chr1 chr2 chr3 chr4 chr5 chr6 chr7 chr8 chr9 chr10 chr11 chr12 chr13 chr14 chr15 chr16 chr17 chr18 chr19 chr20 chr21 chr22 chrX chrY" : "1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 X Y" + + """ + bcftools \\ + view \\ + $vcf \\ + $regions > ${prefix}.vcf + + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/local/bgzip_tabix.nf b/modules/local/bgzip_tabix.nf new file mode 100644 index 0000000..887da5f --- /dev/null +++ b/modules/local/bgzip_tabix.nf @@ -0,0 +1,47 @@ +process BGZIP_TABIX { + tag "$meta.id" + label 'process_single' + + conda "" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta),val(meta2), path(vcf) + + output: + tuple val(meta),val(meta2), path("*.vcf.gz"), path("*.tbi"), emit: gz_tbi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def zipname = vcf.getBaseName() + ".temp.vcf.gz" + + if (vcf.getExtension() == "gz"){ + """ + cp $vcf $zipname + tabix -p vcf $zipname + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + } + else{ + """ + bgzip ${args2} --threads ${task.cpus} -c $vcf > $zipname + tabix -p vcf $zipname + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + } +} diff --git a/modules/local/extract_main.nf b/modules/local/extract_main.nf new file mode 100644 index 0000000..819b077 --- /dev/null +++ b/modules/local/extract_main.nf @@ -0,0 +1,33 @@ +process EXTRACT_MAIN { + tag "$meta.id" + label 'process_single' + + conda "" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta), path(bed) + + output: + tuple val(meta), path("*.txt"), emit: chr_list + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def name = bed.getBaseName() + + """ + cat $bed | awk '{print \$1}' | uniq > ${name}.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + +} diff --git a/modules/local/gridss_annotation.nf b/modules/local/gridss_annotation.nf new file mode 100644 index 0000000..317f5a6 --- /dev/null +++ b/modules/local/gridss_annotation.nf @@ -0,0 +1,60 @@ +// UNTESTED +process GRIDSS_ANNOTATION { + tag "$meta.id $meta2.caller" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gridss:2.13.2--h270b39a_0': + 'quay.io/biocontainers/gridss:2.13.2--h270b39a_0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(index) + tuple path(fasta), path(fasta_fai) + + output: + tuple val(meta),val(meta2), path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def genome = params.genome.contains("38") ? "hg38": "hg19" + def VERSION = '2.13.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + + if (meta2.caller == "gridss"){ + """ + bgzip -d $vcf -c > unzziped.vcf + simple_event-annotator.R \\ + unzziped.vcf \\ + ${prefix}.vcf \\ + ${genome} + + bgzip --threads ${task.cpus} -c ${prefix}.vcf > ${prefix}.anno.vcf.gz + tabix -p vcf ${prefix}.anno.vcf.gz + + rm unzziped.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gridss: ${VERSION} + END_VERSIONS + """ + } + else{ + """ + cp $vcf ${prefix}.vcf.gz + cp $index ${prefix}.vcf.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gridss: ${VERSION} + END_VERSIONS + """ + + } + +} diff --git a/modules/local/main_chroms.nf b/modules/local/main_chroms.nf new file mode 100644 index 0000000..3078ef1 --- /dev/null +++ b/modules/local/main_chroms.nf @@ -0,0 +1,34 @@ +process MAIN_CHROMS { + tag "$meta.id" + label 'process_single' + + conda "" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta), path(fai) + + output: + tuple val(meta), path("*.sizes"), emit: sizes + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def name = fai.getBaseName() + + """ + cut -f 1,2 $fai > size + head -n24 size > ${name}.sizes + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + +} diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 87d88f4..6424a24 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK { conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" + 'quay.io/biocontainers/python:3.8.3' }" input: path samplesheet @@ -17,7 +17,7 @@ process SAMPLESHEET_CHECK { when: task.ext.when == null || task.ext.when - script: // This script is bundled with the pipeline, in nf-core/variantbenchmarking/bin/ + script: // This script is bundled with the pipeline, in nf-core/benchmark/bin/ """ check_samplesheet.py \\ $samplesheet \\ diff --git a/modules/local/truvari_phab.nf b/modules/local/truvari_phab.nf new file mode 100644 index 0000000..ea65b3a --- /dev/null +++ b/modules/local/truvari_phab.nf @@ -0,0 +1,42 @@ +process TRUVARI_PHAB { + tag "$meta.id $meta2.caller" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'docker://kubran/truvari:v4.3.0': + 'kubran/truvari:v4.3.0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple path(fasta), path(fai) + + output: + tuple val(meta), val(meta2), path("*.vcf.gz") , emit: harmon + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions = bed ? "--region $bed" : "" + + """ + truvari phab \\ + --base ${truth_vcf} \\ + --comp ${vcf} \\ + --bSample $meta.id \\ + --cSample $meta.id \\ + --reference ${fasta} \\ + --output ${prefix}.vcf.gz \\ + ${regions} \\ + ${args} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + truvari: \$(echo \$(truvari version 2>&1) | sed 's/^Truvari v//' )) + END_VERSIONS + """ +} diff --git a/modules/local/truvari_refine.nf b/modules/local/truvari_refine.nf new file mode 100644 index 0000000..e10d618 --- /dev/null +++ b/modules/local/truvari_refine.nf @@ -0,0 +1,38 @@ +process TRUVARI_REFINE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'docker://kubran/truvari:v4.3.0': + 'kubran/truvari:v4.3.0' }" + + input: + tuple val(meta), val(meta2), path(bench) + each path(bed) + tuple path(fasta), path(fai) + + output: + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + truvari refine \\ + --use-original-vcfs \\ + --reference $fasta \\ + --regions $bed \\ + $bench + . + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + truvari: \$(echo \$(truvari version 2>&1) | sed 's/^Truvari v//' )) + END_VERSIONS + """ +} diff --git a/modules/local/vcf_genotype_annotator.nf b/modules/local/vcf_genotype_annotator.nf new file mode 100644 index 0000000..9fa366a --- /dev/null +++ b/modules/local/vcf_genotype_annotator.nf @@ -0,0 +1,36 @@ +process VCF_GENOTYPE_ANNOTATOR { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'ddocker://griffithlab/vatools:5.1.10' + 'griffithlab/vatools:5.1.10' }" + + input: + tuple val(meta),val(meta2), path(vcf) + + output: + tuple val(meta),val(meta2), path("*.{vcf}"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + vcf-genotype-annotator \\ + $vcf \\ + ${meta.id} \\ + $args + -o ${prefix}.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ +} diff --git a/modules/local/vcfdist.nf b/modules/local/vcfdist.nf new file mode 100644 index 0000000..0ae9939 --- /dev/null +++ b/modules/local/vcfdist.nf @@ -0,0 +1,41 @@ +process VCFDIST { + tag "$meta.id $meta2.caller" + label 'process_single' + + conda "" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'docker://timd1/vcfdist:v2.3.2' : + 'timd1/vcfdist:v2.3.2' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple path(fasta), path(fai) + + output: + tuple val(meta), path("*.tsv,vcf"), emit: bench + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions = bed ? "-b $bed" : "" + + """ + vcfdist \\ + ${vcf} \\ + ${truth_vcf} \\ + $fasta \\ + -p ${prefix} \\ + ${regions} \\ + ${args} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + vcfdist: \$(echo \$(vcfdist --version 2>&1) | sed 's/^.*vcfdist v//') + END_VERSIONS + """ + +} diff --git a/modules/local/wittyer.nf b/modules/local/wittyer.nf new file mode 100644 index 0000000..20db34a --- /dev/null +++ b/modules/local/wittyer.nf @@ -0,0 +1,53 @@ +process WITTYER { + tag "$meta.id $meta2.caller" + label 'process_single' + + conda "" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'docker://kubran/wittyer:0.3.3.0' : + 'kubran/wittyer:0.3.3.0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + path(config) + + output: + tuple val(meta), path("*ConfigFileUsed.json") , emit: config + tuple val(meta), path("*.Stats.json") , emit: report + tuple val(meta), path("*eval.vcf.gz") , emit: bench_vcf + tuple val(meta), path("*eval.vcf.gz.tbi") , emit: bench_vcf_gzi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions = bed ? "--includeBed=$bed" : "" + def config = config ? "--configFile=$config" : "" + + """ + mkdir bench + dotnet /opt/Wittyer/Wittyer.dll \\ + --truthVcf=${truth_vcf} \\ + --inputVcf=${vcf} \\ + --outputDirectory=bench \\ + ${regions} \\ + ${config} \\ + ${args} + + mv bench/Wittyer.ConfigFileUsed.json ${prefix}.ConfigFileUsed.json + mv bench/Wittyer.Stats.json ${prefix}.Stats.json + mv bench/*.vcf.gz ${prefix}.eval.vcf.gz + mv bench/*.vcf.gz.tbi ${prefix}.eval.vcf.gz.tbi + + rm -rf bench + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wittyer: 0.3.3.0 + END_VERSIONS + """ + +} diff --git a/modules/nf-core/bcftools/annotate/environment.yml b/modules/nf-core/bcftools/annotate/environment.yml new file mode 100644 index 0000000..273ffff --- /dev/null +++ b/modules/nf-core/bcftools/annotate/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_annotate +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.17 diff --git a/modules/nf-core/bcftools/annotate/main.nf b/modules/nf-core/bcftools/annotate/main.nf new file mode 100644 index 0000000..05b9c63 --- /dev/null +++ b/modules/nf-core/bcftools/annotate/main.nf @@ -0,0 +1,63 @@ +process BCFTOOLS_ANNOTATE { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': + 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" + + input: + tuple val(meta), path(input), path(index), path(annotations), path(annotations_index), path(header_lines) + + output: + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def header_file = header_lines ? "--header-lines ${header_lines}" : '' + def annotations_file = annotations ? "--annotations ${annotations}" : '' + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + if ("$input" == "${prefix}.${extension}") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + """ + bcftools \\ + annotate \\ + $args \\ + $annotations_file \\ + $header_file \\ + --output ${prefix}.${extension} \\ + --threads $task.cpus \\ + $input + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/annotate/meta.yml b/modules/nf-core/bcftools/annotate/meta.yml new file mode 100644 index 0000000..f3aa463 --- /dev/null +++ b/modules/nf-core/bcftools/annotate/meta.yml @@ -0,0 +1,56 @@ +name: bcftools_annotate +description: Add or remove annotations. +keywords: + - bcftools + - annotate + - vcf + - remove + - add +tools: + - annotate: + description: Add or remove annotations. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: https://samtools.github.io/bcftools/bcftools.html#annotate + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: Query VCF or BCF file, can be either uncompressed or compressed + - index: + type: file + description: Index of the query VCF or BCF file + - annotations: + type: file + description: Bgzip-compressed file with annotations + - annotations_index: + type: file + description: Index of the annotations file + - header_lines: + type: file + description: Contains lines to append to the output VCF header +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: Compressed annotated VCF file + pattern: "*{vcf,vcf.gz,bcf,bcf.gz}" +authors: + - "@projectoriented" + - "@ramprasadn" +maintainers: + - "@projectoriented" + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/isec/environment.yml b/modules/nf-core/bcftools/isec/environment.yml new file mode 100644 index 0000000..e798a40 --- /dev/null +++ b/modules/nf-core/bcftools/isec/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_isec +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/isec/main.nf b/modules/nf-core/bcftools/isec/main.nf new file mode 100644 index 0000000..64aae32 --- /dev/null +++ b/modules/nf-core/bcftools/isec/main.nf @@ -0,0 +1,33 @@ +process BCFTOOLS_ISEC { + tag "$meta.id $meta.caller" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta),val(meta2), path(vcfs), path(tbis) + + output: + tuple val(meta),val(meta2), path("${prefix}"), emit: results + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + """ + bcftools isec \\ + $args \\ + -p $prefix \\ + *.vcf.gz + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/isec/meta.yml b/modules/nf-core/bcftools/isec/meta.yml new file mode 100644 index 0000000..94f4256 --- /dev/null +++ b/modules/nf-core/bcftools/isec/meta.yml @@ -0,0 +1,52 @@ +name: bcftools_isec +description: Apply set operations to VCF files +keywords: + - variant calling + - intersect + - union + - complement + - VCF +tools: + - isec: + description: | + Computes intersections, unions and complements of VCF files. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcfs: + type: list + description: | + List containing 2 or more vcf files + e.g. [ 'file1.vcf', 'file2.vcf' ] + - tbis: + type: list + description: | + List containing the tbi index files corresponding to the vcfs input files + e.g. [ 'file1.vcf.tbi', 'file2.vcf.tbi' ] +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - results: + type: directory + description: Folder containing the set operations results perform on the vcf files + pattern: "${prefix}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/bcftools/isec/tests/main.nf.test b/modules/nf-core/bcftools/isec/tests/main.nf.test new file mode 100644 index 0000000..89d5335 --- /dev/null +++ b/modules/nf-core/bcftools/isec/tests/main.nf.test @@ -0,0 +1,44 @@ +nextflow_process { + + name "Test Process BCFTOOLS_ISEC" + script "../main.nf" + process "BCFTOOLS_ISEC" + + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/isec" + + config "./nextflow.config" + + test("sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]]") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test2_vcf_gz'], checkIfExists: true)], + [ + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test2_vcf_gz_tbi'], checkIfExists: true)] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.results, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bcftools/isec/tests/main.nf.test.snap b/modules/nf-core/bcftools/isec/tests/main.nf.test.snap new file mode 100644 index 0000000..919809b --- /dev/null +++ b/modules/nf-core/bcftools/isec/tests/main.nf.test.snap @@ -0,0 +1,25 @@ +{ + "sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]]": { + "content": [ + [ + [ + { + "id": "test" + }, + [ + "0000.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee", + "0000.vcf.gz.tbi:md5,7f005943c935f2b55ba3f9d4802aa09f", + "0001.vcf.gz:md5,5937e33da388e9f6992ea0b44c5c2629", + "0001.vcf.gz.tbi:md5,2cdcee9edc71f6d84325d34d78d445cc", + "README.txt:md5,10fc33b66522645600d44afbd41fb792", + "sites.txt:md5,1cea3fbde7f6d3c97f3d39036f9690df" + ] + ] + ], + [ + "versions.yml:md5,c9e98dd502f38110979feabbee9937df" + ] + ], + "timestamp": "2023-11-29T14:00:22.623656731" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/isec/tests/nextflow.config b/modules/nf-core/bcftools/isec/tests/nextflow.config new file mode 100644 index 0000000..3212fe9 --- /dev/null +++ b/modules/nf-core/bcftools/isec/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '--nfiles +2 --output-type z --no-version' +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/isec/tests/tags.yml b/modules/nf-core/bcftools/isec/tests/tags.yml new file mode 100644 index 0000000..c0fb5a1 --- /dev/null +++ b/modules/nf-core/bcftools/isec/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/isec: + - "modules/nf-core/bcftools/isec/**" diff --git a/modules/nf-core/bcftools/norm/environment.yml b/modules/nf-core/bcftools/norm/environment.yml new file mode 100644 index 0000000..74e94f2 --- /dev/null +++ b/modules/nf-core/bcftools/norm/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_norm +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.17 diff --git a/modules/nf-core/bcftools/norm/main.nf b/modules/nf-core/bcftools/norm/main.nf new file mode 100644 index 0000000..379acdb --- /dev/null +++ b/modules/nf-core/bcftools/norm/main.nf @@ -0,0 +1,63 @@ +process BCFTOOLS_NORM { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': + 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(index) + tuple path(fasta), path(fai) + tuple val(meta3), path(regions) + + output: + tuple val(meta),val(meta2), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf.gz" + def region = regions ? "-R ${regions}" : "" + + """ + bcftools norm \\ + --fasta-ref ${fasta} \\ + --output ${prefix}.${extension}\\ + $args \\ + --threads $task.cpus \\ + $region \\ + ${vcf} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf.gz" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/norm/meta.yml b/modules/nf-core/bcftools/norm/meta.yml new file mode 100644 index 0000000..1f3e1b6 --- /dev/null +++ b/modules/nf-core/bcftools/norm/meta.yml @@ -0,0 +1,61 @@ +name: bcftools_norm +description: Normalize VCF file +keywords: + - normalize + - norm + - variant calling + - VCF +tools: + - norm: + description: | + Normalize VCF files. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + The vcf file to be normalized + e.g. 'file1.vcf' + pattern: "*.{vcf,vcf.gz}" + - tbi: + type: file + description: | + An optional index of the VCF file (for when the VCF is compressed) + pattern: "*.vcf.gz.tbi" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] + - fasta: + type: file + description: FASTA reference file + pattern: "*.{fasta,fa}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: One of uncompressed VCF (.vcf), compressed VCF (.vcf.gz), compressed BCF (.bcf.gz) or uncompressed BCF (.bcf) normalized output file + pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@abhi18av" + - "@ramprasadn" +maintainers: + - "@abhi18av" + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/reheader/environment.yml b/modules/nf-core/bcftools/reheader/environment.yml new file mode 100644 index 0000000..aab0dc9 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_reheader +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/reheader/main.nf b/modules/nf-core/bcftools/reheader/main.nf new file mode 100644 index 0000000..a60248c --- /dev/null +++ b/modules/nf-core/bcftools/reheader/main.nf @@ -0,0 +1,68 @@ +process BCFTOOLS_REHEADER { + tag "$meta.id $meta2.caller" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(index) + tuple path(fasta), path(fai) + + output: + tuple val(meta),val(meta2), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def fai_argument = fai ? "--fai $fai" : "" + def args2 = task.ext.args2 ?: '--output-type z' + def extension = args2.contains("--output-type b") || args2.contains("-Ob") ? "bcf.gz" : + args2.contains("--output-type u") || args2.contains("-Ou") ? "bcf" : + args2.contains("--output-type z") || args2.contains("-Oz") ? "vcf.gz" : + args2.contains("--output-type v") || args2.contains("-Ov") ? "vcf" : + "vcf" + """ + echo ${prefix} > sample.txt + + bcftools \\ + reheader \\ + $fai_argument \\ + --samples sample.txt \\ + $args \\ + --threads $task.cpus \\ + $vcf \\ + | bcftools view \\ + $args2 \\ + --output ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args2 = task.ext.args2 ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + + def extension = args2.contains("--output-type b") || args2.contains("-Ob") ? "bcf.gz" : + args2.contains("--output-type u") || args2.contains("-Ou") ? "bcf" : + args2.contains("--output-type z") || args2.contains("-Oz") ? "vcf.gz" : + args2.contains("--output-type v") || args2.contains("-Ov") ? "vcf" : + "vcf" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/reheader/meta.yml b/modules/nf-core/bcftools/reheader/meta.yml new file mode 100644 index 0000000..690d4ea --- /dev/null +++ b/modules/nf-core/bcftools/reheader/meta.yml @@ -0,0 +1,63 @@ +name: bcftools_reheader +description: Reheader a VCF file +keywords: + - reheader + - vcf + - update header +tools: + - reheader: + description: | + Modify header of VCF/BCF files, change sample names. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://samtools.github.io/bcftools/bcftools.html#reheader + doi: 10.1093/gigascience/giab008 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF/BCF file + pattern: "*.{vcf.gz,vcf,bcf}" + - header: + type: file + description: New header to add to the VCF + pattern: "*.{header.txt}" + - samples: + type: file + description: File containing sample names to update (one sample per line) + pattern: "*.{samples.txt}" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] + - fai: + type: file + description: Fasta index to update header sequences with + pattern: "*.{fai}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: VCF with updated header, bgzipped per default + pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" +authors: + - "@bjohnnyd" + - "@jemten" + - "@ramprasadn" +maintainers: + - "@bjohnnyd" + - "@jemten" + - "@ramprasadn" diff --git a/modules/nf-core/bcftools/reheader/tests/bcf.config b/modules/nf-core/bcftools/reheader/tests/bcf.config new file mode 100644 index 0000000..2b7dff5 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/bcf.config @@ -0,0 +1,4 @@ +process { + ext.args2 = { "--no-version --output-type b" } + ext.prefix = "tested" +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/reheader/tests/main.nf.test b/modules/nf-core/bcftools/reheader/tests/main.nf.test new file mode 100644 index 0000000..f3200cb --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/main.nf.test @@ -0,0 +1,197 @@ +nextflow_process { + + name "Test Process BCFTOOLS_REHEADER" + script "../main.nf" + process "BCFTOOLS_REHEADER" + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/reheader" + + test("sarscov2 - [vcf, [], []], fai - vcf output") { + + config "./vcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - vcf.gz output") { + + config "./vcf.gz.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - bcf output") { + + config "./bcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, header, []], []") { + + config "./vcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], samples], fai") { + + config "./vcf.config" + when { + + process { + """ + ch_no_samples = Channel.of([ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [] + ]) + ch_samples = Channel.of(["samples.txt", "new_name"]) + .collectFile(newLine:true) + input[0] = ch_no_samples.combine(ch_samples) + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - stub") { + + options "-stub" + config "./vcf.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.vcf[0][1]).name, + process.out.versions, + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap b/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap new file mode 100644 index 0000000..112736a --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap @@ -0,0 +1,166 @@ +{ + "sarscov2 - [vcf, [], []], fai - vcf output": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:05:44.058376693" + }, + "sarscov2 - [vcf, [], []], fai - bcf output": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.bcf.gz:md5,c31d9afd8614832c2a46d9a55682c97a" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.bcf.gz:md5,c31d9afd8614832c2a46d9a55682c97a" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:06:03.793372514" + }, + "sarscov2 - [vcf, [], []], fai - vcf.gz output": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:05:53.954090441" + }, + "sarscov2 - [vcf, [], []], fai - stub": { + "content": [ + "tested.vcf", + [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + ], + "timestamp": "2023-11-29T13:06:33.549685303" + }, + "sarscov2 - [vcf, [], samples], fai": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,c64c373c10b0be24b29d6f18708ec1e8" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,c64c373c10b0be24b29d6f18708ec1e8" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:06:23.474745156" + }, + "sarscov2 - [vcf, header, []], []": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,3189bc9a720d5d5d3006bf72d91300cb" + ] + ], + "1": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "tested.vcf:md5,3189bc9a720d5d5d3006bf72d91300cb" + ] + ], + "versions": [ + "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + ] + } + ], + "timestamp": "2023-11-29T13:06:13.841648691" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/reheader/tests/tags.yml b/modules/nf-core/bcftools/reheader/tests/tags.yml new file mode 100644 index 0000000..c252941 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/reheader: + - modules/nf-core/bcftools/reheader/** diff --git a/modules/nf-core/bcftools/reheader/tests/vcf.config b/modules/nf-core/bcftools/reheader/tests/vcf.config new file mode 100644 index 0000000..820f2ae --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/vcf.config @@ -0,0 +1,4 @@ +process { + ext.args2 = { "--no-version" } + ext.prefix = "tested" +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/reheader/tests/vcf.gz.config b/modules/nf-core/bcftools/reheader/tests/vcf.gz.config new file mode 100644 index 0000000..c3031c3 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/vcf.gz.config @@ -0,0 +1,4 @@ +process { + ext.args2 = { "--no-version --output-type z" } + ext.prefix = "tested" +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml new file mode 100644 index 0000000..89cf911 --- /dev/null +++ b/modules/nf-core/bcftools/sort/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_sort +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf new file mode 100644 index 0000000..a739b09 --- /dev/null +++ b/modules/nf-core/bcftools/sort/main.nf @@ -0,0 +1,63 @@ +process BCFTOOLS_SORT { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta), val(meta2), path(vcf) + + output: + tuple val(meta), val(meta2), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf + tuple val(meta), val(meta2), path("*.{tbi}") , emit: index + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + """ + bcftools \\ + sort \\ + --output ${prefix}.${extension} \\ + --temp-dir . \\ + --write-index \\ + $args \\ + $vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml new file mode 100644 index 0000000..84747c6 --- /dev/null +++ b/modules/nf-core/bcftools/sort/meta.yml @@ -0,0 +1,42 @@ +name: bcftools_sort +description: Sorts VCF files +keywords: + - sorting + - VCF + - variant calling +tools: + - sort: + description: Sort VCF files by coordinates. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + tool_dev_url: https://github.com/samtools/bcftools + doi: "10.1093/bioinformatics/btp352" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: The VCF/BCF file to be sorted + pattern: "*.{vcf.gz,vcf,bcf}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: Sorted VCF file + pattern: "*.{vcf.gz}" +authors: + - "@Gwennid" +maintainers: + - "@Gwennid" diff --git a/modules/nf-core/bcftools/stats/environment.yml b/modules/nf-core/bcftools/stats/environment.yml new file mode 100644 index 0000000..1a96952 --- /dev/null +++ b/modules/nf-core/bcftools/stats/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_stats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/stats/main.nf b/modules/nf-core/bcftools/stats/main.nf new file mode 100644 index 0000000..8a38397 --- /dev/null +++ b/modules/nf-core/bcftools/stats/main.nf @@ -0,0 +1,60 @@ +process BCFTOOLS_STATS { + tag "$meta.id $meta2.caller" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta), val(meta2), path(vcf), path(index) + tuple val(meta3), path(regions) + tuple val(meta4), path(targets) + tuple val(meta5), path(samples) + tuple val(meta6), path(exons) + tuple val(meta7), path(fasta) + + output: + tuple val(meta),val(meta2), path("*stats.txt"), emit: stats + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions_file = regions ? "--regions-file ${regions}" : "" + def targets_file = targets ? "--targets-file ${targets}" : "" + def samples_file = samples ? "--samples-file ${samples}" : "" + def reference_fasta = fasta ? "--fasta-ref ${fasta}" : "" + def exons_file = exons ? "--exons ${exons}" : "" + """ + bcftools stats \\ + $args \\ + $regions_file \\ + $targets_file \\ + $samples_file \\ + $reference_fasta \\ + $exons_file \\ + $vcf > ${prefix}.bcftools_stats.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.bcftools_stats.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/stats/meta.yml b/modules/nf-core/bcftools/stats/meta.yml new file mode 100644 index 0000000..7ea2103 --- /dev/null +++ b/modules/nf-core/bcftools/stats/meta.yml @@ -0,0 +1,77 @@ +name: bcftools_stats +description: Generates stats from VCF files +keywords: + - variant calling + - stats + - VCF +tools: + - stats: + description: | + Parses VCF or BCF and produces text file stats which is suitable for + machine processing and can be plotted using plot-vcfstats. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF input file + pattern: "*.{vcf}" + - tbi: + type: file + description: | + The tab index for the VCF file to be inspected. Optional: only required when parameter regions is chosen. + pattern: "*.tbi" + - regions: + type: file + description: | + Optionally, restrict the operation to regions listed in this file. (VCF, BED or tab-delimited) + - targets: + type: file + description: | + Optionally, restrict the operation to regions listed in this file (doesn't rely upon tbi index files) + - samples: + type: file + description: | + Optional, file of sample names to be included or excluded. + e.g. 'file.tsv' + - exons: + type: file + description: | + Tab-delimited file with exons for indel frameshifts (chr,beg,end; 1-based, inclusive, optionally bgzip compressed). + e.g. 'exons.tsv.gz' + - fasta: + type: file + description: | + Faidx indexed reference sequence file to determine INDEL context. + e.g. 'reference.fa' +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - stats: + type: file + description: Text output file containing stats + pattern: "*_{stats.txt}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@SusiJo" + - "@TCLamnidis" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@SusiJo" + - "@TCLamnidis" diff --git a/modules/nf-core/bcftools/view/environment.yml b/modules/nf-core/bcftools/view/environment.yml new file mode 100644 index 0000000..8937c6d --- /dev/null +++ b/modules/nf-core/bcftools/view/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_view +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf new file mode 100644 index 0000000..63b44c8 --- /dev/null +++ b/modules/nf-core/bcftools/view/main.nf @@ -0,0 +1,55 @@ +process BCFTOOLS_VIEW { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta3), path(regions) + path(targets) + path(samples) + + output: + tuple val(meta),val(meta2), path("*.gz") , emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions_file = regions ? "--regions-file ${regions}" : "" + def targets_file = targets ? "--targets-file ${targets}" : "" + def samples_file = samples ? "--samples-file ${samples}" : "" + """ + bcftools view \\ + --output ${prefix}.vcf.gz \\ + ${regions_file} \\ + ${targets_file} \\ + ${samples_file} \\ + $args \\ + --threads $task.cpus \\ + ${vcf} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/view/meta.yml b/modules/nf-core/bcftools/view/meta.yml new file mode 100644 index 0000000..eaa12b5 --- /dev/null +++ b/modules/nf-core/bcftools/view/meta.yml @@ -0,0 +1,64 @@ +name: bcftools_view +description: View, subset and filter VCF or BCF files by position and filtering expression. Convert between VCF and BCF +keywords: + - variant calling + - view + - bcftools + - VCF +tools: + - view: + description: | + View, subset and filter VCF or BCF files by position and filtering expression. Convert between VCF and BCF + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + The vcf file to be inspected. + e.g. 'file.vcf' + - index: + type: file + description: | + The tab index for the VCF file to be inspected. + e.g. 'file.tbi' + - regions: + type: file + description: | + Optionally, restrict the operation to regions listed in this file. + e.g. 'file.vcf' + - targets: + type: file + description: | + Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files) + e.g. 'file.vcf' + - samples: + type: file + description: | + Optional, file of sample names to be included or excluded. + e.g. 'file.tsv' +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF normalized output file + pattern: "*.{vcf.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@abhi18av" +maintainers: + - "@abhi18av" diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test b/modules/nf-core/bcftools/view/tests/main.nf.test new file mode 100644 index 0000000..c285674 --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/main.nf.test @@ -0,0 +1,103 @@ +nextflow_process { + + name "Test Process BCFTOOLS_VIEW" + script "../main.nf" + process "BCFTOOLS_VIEW" + + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/view" + + config "./nextflow.config" + + test("sarscov2 - [vcf, tbi], [], [], []") { + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [vcf, tbi], vcf, tsv, []") { + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + ] + input[1] = file(params.test_data['sarscov2']['illumina']['test3_vcf_gz'], checkIfExists: true) + input[2] = file(params.test_data['sarscov2']['illumina']['test2_vcf_targets_tsv_gz'], checkIfExists: true) + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [vcf, tbi], [], [], [] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.vcf[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test.snap b/modules/nf-core/bcftools/view/tests/main.nf.test.snap new file mode 100644 index 0000000..049ac25 --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/main.nf.test.snap @@ -0,0 +1,45 @@ +{ + "sarscov2 - [vcf, tbi], vcf, tsv, []": { + "content": [ + [ + [ + { + "id": "out", + "single_end": false + }, + "out.vcf.gz:md5,3c47ba1a6aa4ef9b3ad800175814d739" + ] + ], + [ + "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + ] + ], + "timestamp": "2023-11-29T14:27:10.724842996" + }, + "sarscov2 - [vcf, tbi], [], [], [] - stub": { + "content": [ + "out.vcf.gz", + [ + "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + ] + ], + "timestamp": "2023-11-29T14:27:17.445846794" + }, + "sarscov2 - [vcf, tbi], [], [], []": { + "content": [ + [ + [ + { + "id": "out", + "single_end": false + }, + "out.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + ] + ], + [ + "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + ] + ], + "timestamp": "2023-11-29T14:27:03.328392594" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/nextflow.config b/modules/nf-core/bcftools/view/tests/nextflow.config new file mode 100644 index 0000000..b05aa50 --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '--no-version' +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/tags.yml b/modules/nf-core/bcftools/view/tests/tags.yml new file mode 100644 index 0000000..43b1f0a --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/view: + - "modules/nf-core/bcftools/view/**" diff --git a/modules/nf-core/bedgovcf/environment.yml b/modules/nf-core/bedgovcf/environment.yml new file mode 100644 index 0000000..61cfbcc --- /dev/null +++ b/modules/nf-core/bedgovcf/environment.yml @@ -0,0 +1,7 @@ +name: bedgovcf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedgovcf=0.1.0 diff --git a/modules/nf-core/bedgovcf/main.nf b/modules/nf-core/bedgovcf/main.nf new file mode 100644 index 0000000..f39f404 --- /dev/null +++ b/modules/nf-core/bedgovcf/main.nf @@ -0,0 +1,49 @@ +process BEDGOVCF { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedgovcf:0.1.0--h9ee0642_0': + 'biocontainers/bedgovcf:0.1.0--h9ee0642_0' }" + + input: + tuple val(meta), path(bed), path(config) + tuple val(meta2), path(fai) + + output: + tuple val(meta), path("*.vcf.gz"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + bedgovcf \\ + $args \\ + --bed $bed \\ + --fai $fai \\ + --config $config \\ + | bgzip --stdout --threads $task.cpus $args2 > ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedgovcf: \$(echo \$(bedgovcf --version 2>&1) | sed 's/^bedgovcf version //' ) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedgovcf: \$(echo \$(bedgovcf --version 2>&1) | sed 's/^bedgovcf version //' ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedgovcf/meta.yml b/modules/nf-core/bedgovcf/meta.yml new file mode 100644 index 0000000..406935e --- /dev/null +++ b/modules/nf-core/bedgovcf/meta.yml @@ -0,0 +1,57 @@ +--- +name: "bedgovcf" +description: Convert a BED file to a VCF file according to a YAML config +keywords: + - bed + - vcf + - conversion + - variants +tools: + - "bedgovcf": + description: "A simple tool to convert BED files to VCF files" + homepage: "https://github.com/nvnieuwk/bedgovcf" + documentation: "https://github.com/nvnieuwk/bedgovcf" + tool_dev_url: "https://github.com/nvnieuwk/bedgovcf" + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - bed: + type: file + description: The BED file to convert to VCF + pattern: "*.bed" + - config: + type: file + description: The config file to use for the conversion + pattern: "*.{yml,yaml}" + - meta2: + type: map + description: | + Groovy Map containing fasta index information + e.g. `[ id:'test', single_end:false ]` + - fai: + type: file + description: The fasta index file + pattern: "*.fai" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: The converted VCF file + pattern: "*.vcf.gz" + +authors: + - "@nvnieuwk" diff --git a/modules/nf-core/bedgovcf/tests/main.nf.test b/modules/nf-core/bedgovcf/tests/main.nf.test new file mode 100644 index 0000000..f55fbb6 --- /dev/null +++ b/modules/nf-core/bedgovcf/tests/main.nf.test @@ -0,0 +1,77 @@ +nextflow_process { + + name "Test Process BEDGOVCF" + script "../main.nf" + process "BEDGOVCF" + tag "modules" + tag "modules_nfcore" + tag "bedgovcf" + + test("homo_sapiens - [bed, config] - fai") { + + // WARNING: md5sum between local tests and GHA tests differ + // Please change them manually after every snapshot update + when { + params { + outdir = $outputDir + } + process { + """ + raw_ch = Channel.of([ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ]) + config = Channel.of(["config.yaml", "header:", " - name: test", " content: test"]) + .collectFile(newLine:true, sort:'index') + input[0] = raw_ch.combine(config) + input[1] = [ + [id:"ref"], + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + + ) + } + } + + + test("homo_sapiens - [bed, config] - fai STUB") { + options "-stub" + when { + params { + outdir = $outputDir + } + process { + """ + raw_ch = Channel.of([ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) + ]) + config = Channel.of(["config.yaml", "header:", " - name: test", " content: test"]) + .collectFile(newLine:true, sort:'index') + input[0] = raw_ch.combine(config) + input[1] = [ + [id:"ref"], + file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + + ) + } + } + +} diff --git a/modules/nf-core/bedgovcf/tests/main.nf.test.snap b/modules/nf-core/bedgovcf/tests/main.nf.test.snap new file mode 100644 index 0000000..2e34fe4 --- /dev/null +++ b/modules/nf-core/bedgovcf/tests/main.nf.test.snap @@ -0,0 +1,60 @@ +{ + "homo_sapiens - [bed, config] - fai STUB": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ] + } + ], + "timestamp": "2023-11-14T12:45:41.931292108" + }, + "homo_sapiens - [bed, config] - fai": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" + ] + ], + "1": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" + ] + ], + "versions": [ + "versions.yml:md5,27e259763ddc44796207f766afb94887" + ] + } + ], + "timestamp": "2023-11-14T12:45:36.218283171" + } +} \ No newline at end of file diff --git a/modules/nf-core/bedgovcf/tests/tags.yml b/modules/nf-core/bedgovcf/tests/tags.yml new file mode 100644 index 0000000..d66b105 --- /dev/null +++ b/modules/nf-core/bedgovcf/tests/tags.yml @@ -0,0 +1,2 @@ +bedgovcf: + - modules/nf-core/bedgovcf/** diff --git a/modules/nf-core/bedtools/coverage/environment.yml b/modules/nf-core/bedtools/coverage/environment.yml new file mode 100644 index 0000000..53a3b4c --- /dev/null +++ b/modules/nf-core/bedtools/coverage/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_coverage +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.30.0 diff --git a/modules/nf-core/bedtools/coverage/main.nf b/modules/nf-core/bedtools/coverage/main.nf new file mode 100644 index 0000000..03b0545 --- /dev/null +++ b/modules/nf-core/bedtools/coverage/main.nf @@ -0,0 +1,39 @@ +process BEDTOOLS_COVERAGE { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--h468198e_3': + 'quay.io/biocontainers/bedtools:2.30.0--h468198e_3' }" + + input: + tuple val(meta), path(input_A), path(input_B) + path genome_file + + output: + tuple val(meta), path("*.bed"), emit: bed + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def reference = genome_file ? "-g ${genome_file} -sorted" : "" + """ + bedtools \\ + coverage \\ + $args \\ + $reference \\ + -a $input_A \\ + -b $input_B \\ + > ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(echo \$(bedtools --version 2>&1) | sed 's/^.*bedtools v//' )) + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/coverage/meta.yml b/modules/nf-core/bedtools/coverage/meta.yml new file mode 100644 index 0000000..d825ca7 --- /dev/null +++ b/modules/nf-core/bedtools/coverage/meta.yml @@ -0,0 +1,57 @@ +name: "bedtools_coverage" +description: computes both the depth and breadth of coverage of features in file B on the features in file A +keywords: + - bedtools + - coverage + - bam + - bed + - gff + - vcf + - histogram +tools: + - "bedtools": + description: "A powerful toolset for genome arithmetic" + homepage: "https://bedtools.readthedocs.io/en/latest/index.html" + documentation: "https://bedtools.readthedocs.io/en/latest/content/bedtools-suite.html" + tool_dev_url: "https://github.com/arq5x/bedtools2" + doi: "10.1093/bioinformatics/btq033" + licence: "['GPL v2', 'MIT']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input_A: + type: file + description: BAM/BED/GFF/VCF file + pattern: "*.{bam,bed,gff,vcf}" + - input_B: + type: file + description: One or more BAM/BED/GFF/VCF file + pattern: "*.{bam,bed,gff,vcf}" + - genome_file: + type: file + description: | + Optional reference genome 2 column file that defines the expected chromosome order + in the input files for use with the -sorted option. + When `genome_file` is provided, `-sorted` option is added to the command. + pattern: "*.{fai,txt,chromsizes}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bed: + type: file + description: File containing coverage of sequence alignments + pattern: "*.bed" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@priyanka-surana" +maintainers: + - "@priyanka-surana" diff --git a/modules/nf-core/bedtools/genomecov/environment.yml b/modules/nf-core/bedtools/genomecov/environment.yml new file mode 100644 index 0000000..574c267 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_genomecov +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.31.0 diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf new file mode 100644 index 0000000..5020882 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/main.nf @@ -0,0 +1,70 @@ +process BEDTOOLS_GENOMECOV { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' : + 'quay.io/biocontainers/bedtools:2.31.0--hf5e1c6e_2' }" + + input: + tuple val(meta), path(intervals), val(scale) + path sizes + val extension + + output: + tuple val(meta), path("*.${extension}"), emit: genomecov + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args_list = args.tokenize() + args += (scale > 0 && scale != 1) ? " -scale $scale" : "" + if (!args_list.contains('-bg') && (scale > 0 && scale != 1)) { + args += " -bg" + } + + def prefix = task.ext.prefix ?: "${meta.id}" + if (intervals.name =~ /\.bam/) { + """ + bedtools \\ + genomecov \\ + -ibam $intervals \\ + $args \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + } else { + """ + bedtools \\ + genomecov \\ + -i $intervals \\ + -g $sizes \\ + $args \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml new file mode 100644 index 0000000..cc32da9 --- /dev/null +++ b/modules/nf-core/bedtools/genomecov/meta.yml @@ -0,0 +1,59 @@ +name: bedtools_genomecov +description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg) summaries of feature coverage (e.g., aligned sequences) for a given genome. +keywords: + - bed + - bam + - genomecov + - bedtools + - histogram +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - intervals: + type: file + description: BAM/BED/GFF/VCF + pattern: "*.{bam|bed|gff|vcf}" + - scale: + type: integer + description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch + - sizes: + type: file + description: Tab-delimited table of chromosome names in the first column and chromosome sizes in the second column + - extension: + type: string + description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - genomecov: + type: file + description: Computed genome coverage file + pattern: "*.${extension}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" + - "@sidorov-si" + - "@chris-cheshire" +maintainers: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" + - "@sidorov-si" + - "@chris-cheshire" diff --git a/modules/nf-core/bedtools/intersect/environment.yml b/modules/nf-core/bedtools/intersect/environment.yml new file mode 100644 index 0000000..f8bb5fb --- /dev/null +++ b/modules/nf-core/bedtools/intersect/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_intersect +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.30.0 diff --git a/modules/nf-core/bedtools/intersect/main.nf b/modules/nf-core/bedtools/intersect/main.nf new file mode 100644 index 0000000..8d6f280 --- /dev/null +++ b/modules/nf-core/bedtools/intersect/main.nf @@ -0,0 +1,59 @@ +process BEDTOOLS_INTERSECT { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' : + 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" + + input: + tuple val(meta), path(intervals1), path(intervals2) + tuple val(meta2), path(chrom_sizes) + + output: + tuple val(meta), path("*.${extension}"), emit: intersect + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + //Extension of the output file. It is set by the user via "ext.suffix" in the config. Corresponds to the file format which depends on arguments (e. g., ".bed", ".bam", ".txt", etc.). + extension = task.ext.suffix ?: "${intervals1.extension}" + def sizes = chrom_sizes ? "-g ${chrom_sizes}" : '' + if ("$intervals1" == "${prefix}.${extension}" || + "$intervals2" == "${prefix}.${extension}") + error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + bedtools \\ + intersect \\ + -a $intervals1 \\ + -b $intervals2 \\ + $args \\ + $sizes \\ + > ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + extension = task.ext.suffix ?: "bed" + if ("$intervals1" == "${prefix}.${extension}" || + "$intervals2" == "${prefix}.${extension}") + error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/intersect/meta.yml b/modules/nf-core/bedtools/intersect/meta.yml new file mode 100644 index 0000000..8d9a08a --- /dev/null +++ b/modules/nf-core/bedtools/intersect/meta.yml @@ -0,0 +1,59 @@ +name: bedtools_intersect +description: Allows one to screen for overlaps between two sets of genomic features. +keywords: + - bed + - intersect + - overlap +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/intersect.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - intervals1: + type: file + description: BAM/BED/GFF/VCF + pattern: "*.{bam|bed|gff|vcf}" + - intervals2: + type: file + description: BAM/BED/GFF/VCF + pattern: "*.{bam|bed|gff|vcf}" + - meta2: + type: map + description: | + Groovy Map containing reference chromosome sizes + e.g. [ id:'test' ] + - chrom_sizes: + type: file + description: Chromosome sizes file + pattern: "*{.sizes,.txt}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - intersect: + type: file + description: File containing the description of overlaps found between the two features + pattern: "*.${extension}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" + - "@sidorov-si" +maintainers: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" + - "@sidorov-si" diff --git a/modules/nf-core/bedtools/merge/environment.yml b/modules/nf-core/bedtools/merge/environment.yml new file mode 100644 index 0000000..961e602 --- /dev/null +++ b/modules/nf-core/bedtools/merge/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_merge +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.31.0 diff --git a/modules/nf-core/bedtools/merge/main.nf b/modules/nf-core/bedtools/merge/main.nf new file mode 100644 index 0000000..153145b --- /dev/null +++ b/modules/nf-core/bedtools/merge/main.nf @@ -0,0 +1,47 @@ +process BEDTOOLS_MERGE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' : + 'quay.io/biocontainers/bedtools:2.31.0--hf5e1c6e_2' }" + + input: + tuple val(meta), path(bed) + + output: + tuple val(meta), path('*.bed'), emit: bed + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if ("$bed" == "${prefix}.bed") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + bedtools \\ + merge \\ + -i $bed \\ + $args \\ + > ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/merge/meta.yml b/modules/nf-core/bedtools/merge/meta.yml new file mode 100644 index 0000000..5565ce4 --- /dev/null +++ b/modules/nf-core/bedtools/merge/meta.yml @@ -0,0 +1,45 @@ +name: bedtools_merge +description: combines overlapping or “book-ended” features in an interval file into a single feature which spans all of the combined features. +keywords: + - bed + - merge + - bedtools + - overlapped bed +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/merge.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bed: + type: file + description: Input BED file + pattern: "*.{bed}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bed: + type: file + description: Overlapped bed file with combined features + pattern: "*.{bed}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" +maintainers: + - "@Emiller88" + - "@sruthipsuresh" + - "@drpatelh" diff --git a/modules/nf-core/bedtools/subtract/environment.yml b/modules/nf-core/bedtools/subtract/environment.yml new file mode 100644 index 0000000..b869a4f --- /dev/null +++ b/modules/nf-core/bedtools/subtract/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_subtract +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.30.0 diff --git a/modules/nf-core/bedtools/subtract/main.nf b/modules/nf-core/bedtools/subtract/main.nf new file mode 100644 index 0000000..028660d --- /dev/null +++ b/modules/nf-core/bedtools/subtract/main.nf @@ -0,0 +1,50 @@ +process BEDTOOLS_SUBTRACT { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' : + 'biocontainers/bedtools:2.30.0--hc088bd4_0' }" + + input: + tuple val(meta), path(intervals1), path(intervals2) + + output: + tuple val(meta), path("*.bed"), emit: bed + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if ("$intervals1" == "${prefix}.bed" || + "$intervals2" == "${prefix}.bed") + error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + bedtools \\ + subtract \\ + -a $intervals1 \\ + -b $intervals2 \\ + $args \\ + > ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/subtract/meta.yml b/modules/nf-core/bedtools/subtract/meta.yml new file mode 100644 index 0000000..0226ff1 --- /dev/null +++ b/modules/nf-core/bedtools/subtract/meta.yml @@ -0,0 +1,45 @@ +name: bedtools_subtract +description: Finds overlaps between two sets of regions (A and B), removes the overlaps from A and reports the remaining portion of A. +keywords: + - bed + - gff + - vcf + - subtract +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/subtract.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - intervals1: + type: file + description: BED/GFF/VCF + pattern: "*.{bed|gff|vcf}" + - intervals2: + type: file + description: BED/GFF/VCF + pattern: "*.{bed|gff|vcf}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bed: + type: file + description: File containing the difference between the two sets of features + patters: "*.bed" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@sidorov-si" +maintainers: + - "@sidorov-si" diff --git a/modules/nf-core/bedtools/unionbedg/environment.yml b/modules/nf-core/bedtools/unionbedg/environment.yml new file mode 100644 index 0000000..895a72f --- /dev/null +++ b/modules/nf-core/bedtools/unionbedg/environment.yml @@ -0,0 +1,7 @@ +name: bedtools_unionbedg +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bedtools=2.31.0 diff --git a/modules/nf-core/bedtools/unionbedg/main.nf b/modules/nf-core/bedtools/unionbedg/main.nf new file mode 100644 index 0000000..68b9054 --- /dev/null +++ b/modules/nf-core/bedtools/unionbedg/main.nf @@ -0,0 +1,50 @@ +process BEDTOOLS_UNIONBEDG { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' : + 'biocontainers/bedtools:2.31.0--hf5e1c6e_2' }" + + input: + tuple val(meta), path(bedgraph) + tuple val(meta2), path(chrom_sizes) + + output: + tuple val(meta), path("*.bed"), emit: bed + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def sizes = chrom_sizes ? "-g ${chrom_sizes}" : '' + bedgraph.collect { if ("$it" == "${prefix}.bed") error "$it has the same name as the output, use \"task.ext.prefix\" to disambiguate!" } + """ + bedtools \\ + unionbedg \\ + -i $bedgraph \\ + $sizes \\ + $args \\ + > ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/bedtools/unionbedg/meta.yml b/modules/nf-core/bedtools/unionbedg/meta.yml new file mode 100644 index 0000000..ee73e8b --- /dev/null +++ b/modules/nf-core/bedtools/unionbedg/meta.yml @@ -0,0 +1,52 @@ +name: bedtools_unionbedg +description: Combines multiple BedGraph files into a single file +keywords: + - bed + - unionBedGraphs + - bedGraph + - comparisons + - combine +tools: + - bedtools: + description: | + A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. + documentation: https://bedtools.readthedocs.io/en/latest/content/tools/slop.html + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bedgraph: + type: file + description: | + Input BedGraph file: four column BED format, with 4th column with numerical values: integer or real, positive or negative + pattern: "*.{bedGraph,bedgraph}" + - meta2: + type: map + description: | + Groovy Map containing meta information for the reference chromosome sizes + e.g. [ id:'test' ] + - chrom_sizes: + type: file + description: Chromosome sizes file + pattern: "*{.sizes,.txt}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bed: + type: file + description: Combined BED file with values from all bedGraph files + pattern: "*.{bed}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@ekushele" +maintainers: + - "@ekushele" diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index 9b3272b..f0c63f6 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index f218761..3c726cf 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index 5f15a5f..9414c32 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index b1e1630..eec1db1 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,12 +31,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } + { assert snapshot(process.out).match() } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 5f59a93..4274ed5 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,33 +1,27 @@ { "Should run without failures": { "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } ], - "timestamp": "2024-01-09T23:01:18.710682" + "timestamp": "2023-11-03T14:43:22.157011" } -} \ No newline at end of file +} diff --git a/modules/nf-core/manta/convertinversion/main.nf b/modules/nf-core/manta/convertinversion/main.nf new file mode 100644 index 0000000..9010c3c --- /dev/null +++ b/modules/nf-core/manta/convertinversion/main.nf @@ -0,0 +1,36 @@ +process MANTA_CONVERTINVERSION { + tag "$meta.id" + label 'process_single' + label 'error_retry' + + conda "bioconda::manta=1.6.0 bioconda::samtools=1.16.1" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-40295ae41112676b05b649e513fe7000675e9b84:a0332aa38645fbb8969567731ce68cfb7f830ec4-0': + 'quay.io/biocontainers/mulled-v2-40295ae41112676b05b649e513fe7000675e9b84:a0332aa38645fbb8969567731ce68cfb7f830ec4-0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(index) + tuple path(fasta), path(fai) + + output: + tuple val(meta),val(meta2), path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf_tabi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + convertInversion.py \$(which samtools) $fasta $vcf | bgzip --threads $task.cpus > ${prefix}.converted.vcf.gz + tabix ${prefix}.converted.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + manta: \$( configManta.py --version ) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) + END_VERSIONS + """ + +} diff --git a/modules/nf-core/manta/convertinversion/meta.yml b/modules/nf-core/manta/convertinversion/meta.yml new file mode 100644 index 0000000..cf9e169 --- /dev/null +++ b/modules/nf-core/manta/convertinversion/meta.yml @@ -0,0 +1,46 @@ +name: "manta_convertinversion" +description: Manta calls structural variants (SVs) and indels from mapped paired-end sequencing reads. This script reformats inversions into single inverted sequence junctions which was the format used in Manta versions <= 1.4.0. +keywords: + - structural variants + - conversion + - indels +tools: + - manta: + description: Structural variant and indel caller for mapped sequencing data + homepage: https://github.com/Illumina/manta + documentation: https://github.com/Illumina/manta/blob/v1.6.0/docs/userGuide/README.md + tool_dev_url: https://github.com/Illumina/manta + doi: "10.1093/bioinformatics/btv710" + licence: ["GPL v3"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF file produces by Manta + pattern: "*.vcf.gz" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: VCF file with reformatted inversions + pattern: "*.vcf.gz" + - tbi: + type: file + description: TBI file produces by Manta + pattern: "*.vcf.gz.tbi" +authors: + - "@FriederikeHanssen" diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b75..e5e14e8 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -1,7 +1,7 @@ -name: multiqc +name: MultiQC channels: - conda-forge - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c4..a908c80 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,14 +25,12 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' - def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ - $logo \\ . cat <<-END_VERSIONS > versions.yml @@ -43,7 +41,7 @@ process MULTIQC { stub: """ - mkdir multiqc_data + touch multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index 45a9bc3..a61223e 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,5 @@ -name: multiqc +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +name: MultiQC description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC diff --git a/modules/nf-core/survivor/filter/environment.yml b/modules/nf-core/survivor/filter/environment.yml new file mode 100644 index 0000000..56839ce --- /dev/null +++ b/modules/nf-core/survivor/filter/environment.yml @@ -0,0 +1,7 @@ +name: survivor_filter +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::survivor=1.0.7 diff --git a/modules/nf-core/survivor/filter/main.nf b/modules/nf-core/survivor/filter/main.nf new file mode 100644 index 0000000..d9c2557 --- /dev/null +++ b/modules/nf-core/survivor/filter/main.nf @@ -0,0 +1,64 @@ +process SURVIVOR_FILTER { + tag "$meta.id $meta2.caller" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/survivor:1.0.7--h9a82719_1': + 'quay.io/biocontainers/survivor:1.0.7--h9a82719_1' }" + + input: + tuple val(meta),val(meta2), path(vcf_file), path(bed) // VCF file to filter and BED file with regions to ignore (NA to disable) + val(minsv) // Min SV size (-1 to disable) + val(maxsv) // Max SV size (-1 to disable) + val(minallelefreq) // Min allele frequency (0-1) + val(minnumreads) // Min number of reads support: RE flag (-1 to disable) + + output: + tuple val(meta),val(meta2), path("*.vcf"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def bed_file = bed ? "${bed}" : "NA" + + if( "$vcf_file" == "${prefix}.vcf" ){ + error "Input and output names are the same, set prefix in module configuration to disambiguate!" + } + """ + SURVIVOR \\ + filter \\ + $vcf_file \\ + $bed_file \\ + $minsv \\ + $maxsv \\ + $minallelefreq \\ + $minnumreads \\ + ${prefix}.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + survivor: \$(echo \$(SURVIVOR 2>&1 | grep "Version" | sed 's/^Version: //')) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def bed_file = bed ? "${bed}" : "NA" + + if( "$vcf_file" == "${prefix}.vcf" ){ + error "Input and output names are the same, set prefix in module configuration to disambiguate!" + } + + """ + touch ${prefix}.vcf + cat <<-END_VERSIONS > versions.yml + "${task.process}": + survivor: \$(echo \$(SURVIVOR 2>&1 | grep "Version" | sed 's/^Version: //')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/survivor/filter/meta.yml b/modules/nf-core/survivor/filter/meta.yml new file mode 100644 index 0000000..4cb2905 --- /dev/null +++ b/modules/nf-core/survivor/filter/meta.yml @@ -0,0 +1,58 @@ +name: "survivor_filter" +description: Filter a vcf file based on size and/or regions to ignore +keywords: + - survivor + - filter + - vcf + - structural variants +tools: + - "survivor": + description: "Toolset for SV simulation, comparison and filtering" + homepage: "https://github.com/fritzsedlazeck/SURVIVOR/wiki" + documentation: "https://github.com/fritzsedlazeck/SURVIVOR/wiki" + tool_dev_url: "https://github.com/fritzsedlazeck/SURVIVOR" + doi: "10.1038/NCOMMS14061" + licence: "['MIT']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF file to filter + pattern: "*.{vcf}" + - bed: + type: file + description: BED file with regions to ignore (NA to disable) + - minsv: + type: integer + description: Min SV size (-1 to disable) + - maxsv: + type: integer + description: Max SV size (-1 to disable) + - minallelefreq: + type: float + description: Min allele frequency (0-1) + - minnumreads: + type: integer + description: Min number of reads support [RE flag (-1 to disable)] +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: Filtered VCF file + pattern: "*.{vcf}" +authors: + - "@LlaneroHiboreo" +maintainers: + - "@LlaneroHiboreo" diff --git a/modules/nf-core/survivor/stats/environment.yml b/modules/nf-core/survivor/stats/environment.yml new file mode 100644 index 0000000..4f64007 --- /dev/null +++ b/modules/nf-core/survivor/stats/environment.yml @@ -0,0 +1,7 @@ +name: survivor_stats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::survivor=1.0.7 diff --git a/modules/nf-core/survivor/stats/main.nf b/modules/nf-core/survivor/stats/main.nf new file mode 100644 index 0000000..528e94c --- /dev/null +++ b/modules/nf-core/survivor/stats/main.nf @@ -0,0 +1,54 @@ +process SURVIVOR_STATS { + tag "$meta.id $meta2.caller" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/survivor:1.0.7--h9a82719_1': + 'quay.io/biocontainers/survivor:1.0.7--h9a82719_1' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(index) + val(minsv) // Min SV size (-1 to disable) + val(maxsv) // Max SV size (-1 to disable) + val(minnumreads) // Min number of reads support: RE flag (-1 to disable) + + output: + tuple val(meta),val(meta2), path("*.stats"), emit: stats + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def prefix = task.ext.prefix ?: "${meta.id}" + def name = vcf.getBaseName() + + """ + gzip -d $vcf + + SURVIVOR \\ + stats \\ + $name \\ + $minsv \\ + $maxsv \\ + $minnumreads \\ + ${prefix}.stats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + survivor: \$(echo \$(SURVIVOR 2>&1 | grep "Version" | sed 's/^Version: //')) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.stats + cat <<-END_VERSIONS > versions.yml + "${task.process}": + survivor: \$(echo \$(SURVIVOR 2>&1 | grep "Version" | sed 's/^Version: //')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/survivor/stats/meta.yml b/modules/nf-core/survivor/stats/meta.yml new file mode 100644 index 0000000..ba450a7 --- /dev/null +++ b/modules/nf-core/survivor/stats/meta.yml @@ -0,0 +1,52 @@ +name: "survivor_stats" +description: Report multipe stats over a VCF file +keywords: + - survivor + - statistics + - vcf + - structural variants +tools: + - "survivor": + description: "Toolset for SV simulation, comparison and filtering" + homepage: "https://github.com/fritzsedlazeck/SURVIVOR/wiki" + documentation: "https://github.com/fritzsedlazeck/SURVIVOR/wiki" + tool_dev_url: "https://github.com/fritzsedlazeck/SURVIVOR" + doi: "10.1038/NCOMMS14061" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF file to filter + pattern: "*.{vcf}" + - minsv: + type: integer + description: Min SV size (-1 to disable) + - maxsv: + type: integer + description: Max SV size (-1 to disable) + - minnumreads: + type: integer + description: Min number of reads support [RE flag (-1 to disable)] +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - stats: + type: file + description: File containing statistics given input VCF file + pattern: "*.{stats}" +authors: + - "@kubranarci" +maintainers: + - "@kubranarci" diff --git a/modules/nf-core/survivor/stats/tests/main.nf.test b/modules/nf-core/survivor/stats/tests/main.nf.test new file mode 100644 index 0000000..e83df57 --- /dev/null +++ b/modules/nf-core/survivor/stats/tests/main.nf.test @@ -0,0 +1,71 @@ +// nf-core modules test survivor/stats +nextflow_process { + + name "Test Process SURVIVOR_STATS" + script "../main.nf" + process "SURVIVOR_STATS" + + tag "modules" + tag "modules_nfcore" + tag "survivor" + tag "survivor/stats" + + test("homo_sapiens - haplotc - vcfgz") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true) + ] + input[1] = -1 + input[2] = -1 + input[3] = -1 + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.stats, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - mutect - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true) + ] + input[1] = -1 + input[2] = -1 + input[3] = -1 + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.stats, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/survivor/stats/tests/main.nf.test.snap b/modules/nf-core/survivor/stats/tests/main.nf.test.snap new file mode 100644 index 0000000..d629dfc --- /dev/null +++ b/modules/nf-core/survivor/stats/tests/main.nf.test.snap @@ -0,0 +1,34 @@ +{ + "homo_sapiens - haplotc - vcfgz": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.stats:md5,023a37c7d6fab374c9bdcf98c011022c" + ] + ], + [ + "versions.yml:md5,35bf44775e74ffec88173cd2093c2928" + ] + ], + "timestamp": "2024-01-15T15:37:42.562568997" + }, + "homo_sapiens - mutect - stub": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.stats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + [ + "versions.yml:md5,35bf44775e74ffec88173cd2093c2928" + ] + ], + "timestamp": "2024-01-15T15:27:09.132671858" + } +} \ No newline at end of file diff --git a/modules/nf-core/survivor/stats/tests/tags.yml b/modules/nf-core/survivor/stats/tests/tags.yml new file mode 100644 index 0000000..762cebe --- /dev/null +++ b/modules/nf-core/survivor/stats/tests/tags.yml @@ -0,0 +1,2 @@ +survivor/stats: + - "modules/nf-core/survivor/stats/**" diff --git a/modules/nf-core/svanalyzer/svbenchmark/environment.yml b/modules/nf-core/svanalyzer/svbenchmark/environment.yml new file mode 100644 index 0000000..12cac54 --- /dev/null +++ b/modules/nf-core/svanalyzer/svbenchmark/environment.yml @@ -0,0 +1,10 @@ +name: svanalyzer_svbenchmark + +channels: + - conda-forge + - bioconda + - defaults + +dependencies: + - bioconda::edlib=1.2.3 + - bioconda::svanalyzer=0.35 diff --git a/modules/nf-core/svanalyzer/svbenchmark/main.nf b/modules/nf-core/svanalyzer/svbenchmark/main.nf new file mode 100644 index 0000000..afbcd9b --- /dev/null +++ b/modules/nf-core/svanalyzer/svbenchmark/main.nf @@ -0,0 +1,65 @@ + +process SVANALYZER_SVBENCHMARK { + tag "$meta.id $meta2.caller" + label 'process_medium' + + conda "bioconda::svanalyzer=0.35" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/svanalyzer:0.35--pl526_0': + 'quay.io/biocontainers/svanalyzer:0.35--pl526_0' }" + + input: + tuple val(meta),val(meta2), path(test), path(test_index), path(truth), path(truth_index), path(bed) + tuple path(fasta), path(fai) + + output: + tuple val(meta),val(meta2), path("*.falsenegatives.vcf"), emit: fns + tuple val(meta),val(meta2), path("*.falsepositives.vcf"), emit: fps + tuple val(meta),val(meta2), path("*.distances") , emit: distances + tuple val(meta),val(meta2), path("*.log") , emit: log + tuple val(meta),val(meta2), path("*.report") , emit: report + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def bed = bed ? "-includebed $bed" : "" + def VERSION = '0.35' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + + """ + svanalyzer \\ + benchmark \\ + $args \\ + --ref $fasta \\ + --test $test \\ + --truth $truth \\ + --prefix $prefix \\ + $bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + svanalyzer: ${VERSION} + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '0.35' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + + """ + touch ${prefix}.falsenegatives.vcf + touch ${prefix}.falsepositives.vcf + touch ${prefix}.distances + touch ${prefix}.log + touch ${prefix}.report + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + svanalyzer: ${VERSION} + END_VERSIONS + """ +} diff --git a/modules/nf-core/svanalyzer/svbenchmark/meta.yml b/modules/nf-core/svanalyzer/svbenchmark/meta.yml new file mode 100644 index 0000000..ba72c3d --- /dev/null +++ b/modules/nf-core/svanalyzer/svbenchmark/meta.yml @@ -0,0 +1,80 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +name: "svanalyzer_svbenchmark" +description: "SVbenchmark compares a set of “test” structural variants in VCF format to a known truth set (also in VCF format) and outputs estimates of sensitivity and specificity." +keywords: + - structural variant + - sv + - benchmarking +tools: + - "svanalyzer": + description: "SVanalyzer: tools for the analysis of structural variation in genomes" + homepage: "https://svanalyzer.readthedocs.io/en/latest/index.html" + documentation: "https://svanalyzer.readthedocs.io/en/latest/index.html" + tool_dev_url: "https://github.com/nhansen/SVanalyzer" + license: "['CC0']" + +input: + - meta: + type: map + description: | + Groovy Map containing test sample information + e.g. `[ id:'test' ]` + - meta2: + type: map + description: | + Groovy Map containing truth sample information + e.g. `[ id:'test2' ]` + - meta3: + type: map + description: | + Groovy Map containing reference genome information + e.g. `[ id:'test3' ]` + - test: + type: file + description: A VCF-formatted file of structural variants to test (required) + pattern: "*.{vcf,vcf.gz}" + - truth: + type: file + description: A VCF-formatted file of variants to compare against (required) + pattern: "*.{vcf,vcf.gz}" + - fasta: + type: file + description: The reference FASTA file for the supplied VCF file or files (required) + pattern: "*.{fa,fasta,fa.gz,fasta.gz}" + - bed: + type: file + description: BED File of regions from which to include variants. Used to filter both test and truth variants. + pattern: "*.{bed}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information inherited from test vcf + e.g. `[ id:'test']` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - fns: + type: file + description: VCF file with False Negatives + pattern: "*.{vcf}" + - fps: + type: file + description: VCF file with False Positives + pattern: "*.{vcf}" + - distances: + type: file + description: TSV file with genomic distances and size differences between structural variants compared + pattern: "*.{distances}" + - log: + type: file + description: LOG file of the run + pattern: "*.{log}" + - report: + type: file + description: Text file reporting RECALL, PRECISION and F1. + pattern: "*.{log}" +authors: + - "@kubranarci" diff --git a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test new file mode 100644 index 0000000..51466f7 --- /dev/null +++ b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test @@ -0,0 +1,85 @@ +nextflow_process { + + name "Test Process SVANALYZER_SVBENCHMARK" + script "../main.nf" + process "SVANALYZER_SVBENCHMARK" + tag "modules" + tag "modules_nfcore" + tag "svanalyzer" + tag "svanalyzer/svbenchmark" + + test("homo_sapiens - illumina - vcf.gz") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true) + ] + input[2] = [ + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + input[3] = [ + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) + ] + input[4] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.fns).match("fns") }, + { assert snapshot(process.out.fps).match("fps") }, + { assert snapshot(process.out.distances).match("distances") }, + { assert snapshot(process.out.report).match("report") }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("homo_sapiens - illumina - vcf.gz - bed") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true) + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true) + ] + input[2] = [ + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + input[3] = [ + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) + ] + input[4] = [ + file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.fns).match("bed_fns") }, + { assert snapshot(process.out.fps).match("bed_fps") }, + { assert snapshot(process.out.distances).match("bed_distances") }, + { assert snapshot(process.out.report).match("bed_report") }, + { assert snapshot(process.out.versions).match("bed_versions") } + ) + } + } +} \ No newline at end of file diff --git a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap new file mode 100644 index 0000000..599697d --- /dev/null +++ b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap @@ -0,0 +1,188 @@ +{ + "homo_sapiens - illumina - vcf.gz": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.falsenegatives.vcf:md5,7a574ad3c614f0c38a6dccb5901ddc05" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" + ] + ], + "2": [ + [ + { + "id": "test" + }, + "test.distances:md5,b6a908b1187f159429dc8c6beb6e6f57" + ] + ], + "3": [ + [ + { + "id": "test" + }, + "test.log:md5,16fd250ae2adcb07c8f101f640fdce81" + ] + ], + "4": [ + [ + { + "id": "test" + }, + "test.report:md5,d0c7db2466442cbdd4f87ad516e00d32" + ] + ], + "5": [ + "versions.yml:md5,6ac08491dbe591d24615c131658cf771" + ], + "distances": [ + [ + { + "id": "test" + }, + "test.distances:md5,b6a908b1187f159429dc8c6beb6e6f57" + ] + ], + "fns": [ + [ + { + "id": "test" + }, + "test.falsenegatives.vcf:md5,7a574ad3c614f0c38a6dccb5901ddc05" + ] + ], + "fps": [ + [ + { + "id": "test" + }, + "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" + ] + ], + "log": [ + [ + { + "id": "test" + }, + "test.log:md5,16fd250ae2adcb07c8f101f640fdce81" + ] + ], + "report": [ + [ + { + "id": "test" + }, + "test.report:md5,d0c7db2466442cbdd4f87ad516e00d32" + ] + ], + "versions": [ + "versions.yml:md5,6ac08491dbe591d24615c131658cf771" + ] + } + ], + "timestamp": "2023-11-24T15:28:29.422156227" + }, + "homo_sapiens - illumina - vcf.gz - bed": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.falsenegatives.vcf:md5,c04105833d5f9acb5ec50af0b89a6b0f" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" + ] + ], + "2": [ + [ + { + "id": "test" + }, + "test.distances:md5,95f7b1679e805979f0c9a4a322cb77f4" + ] + ], + "3": [ + [ + { + "id": "test" + }, + "test.log:md5,75f05aac48afd818f2d5e8a3144116ea" + ] + ], + "4": [ + [ + { + "id": "test" + }, + "test.report:md5,aaa9225dd6e261951c020e23473375b9" + ] + ], + "5": [ + "versions.yml:md5,6ac08491dbe591d24615c131658cf771" + ], + "distances": [ + [ + { + "id": "test" + }, + "test.distances:md5,95f7b1679e805979f0c9a4a322cb77f4" + ] + ], + "fns": [ + [ + { + "id": "test" + }, + "test.falsenegatives.vcf:md5,c04105833d5f9acb5ec50af0b89a6b0f" + ] + ], + "fps": [ + [ + { + "id": "test" + }, + "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" + ] + ], + "log": [ + [ + { + "id": "test" + }, + "test.log:md5,75f05aac48afd818f2d5e8a3144116ea" + ] + ], + "report": [ + [ + { + "id": "test" + }, + "test.report:md5,aaa9225dd6e261951c020e23473375b9" + ] + ], + "versions": [ + "versions.yml:md5,6ac08491dbe591d24615c131658cf771" + ] + } + ], + "timestamp": "2023-11-24T15:29:05.547215282" + } +} \ No newline at end of file diff --git a/modules/nf-core/svanalyzer/svbenchmark/tests/tags.yml b/modules/nf-core/svanalyzer/svbenchmark/tests/tags.yml new file mode 100644 index 0000000..2a3bc9c --- /dev/null +++ b/modules/nf-core/svanalyzer/svbenchmark/tests/tags.yml @@ -0,0 +1,2 @@ +svanalyzer/svbenchmark: + - "modules/nf-core/svanalyzer/svbenchmark/**" diff --git a/modules/nf-core/svync/environment.yml b/modules/nf-core/svync/environment.yml new file mode 100644 index 0000000..fb7a1db --- /dev/null +++ b/modules/nf-core/svync/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "svync" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::svync=0.1.2" diff --git a/modules/nf-core/svync/main.nf b/modules/nf-core/svync/main.nf new file mode 100644 index 0000000..d28e526 --- /dev/null +++ b/modules/nf-core/svync/main.nf @@ -0,0 +1,40 @@ +process SVYNC { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/svync:0.1.2--h9ee0642_0': + 'quay.io/biocontainers/svync:0.1.2--h9ee0642_0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(tbi), path(config) + + output: + tuple val(meta),val(meta2), path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + if ("$vcf" == "${prefix}.vcf.gz") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + + """ + svync \\ + $args \\ + --config $config \\ + --input $vcf \\ + | bgzip --threads $task.cpus $args2 > ${prefix}.vcf.gz + + tabix ${prefix}.vcf.gz + cat <<-END_VERSIONS > versions.yml + "${task.process}": + svync: \$(svync --version | sed 's/svync version //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/svync/meta.yml b/modules/nf-core/svync/meta.yml new file mode 100644 index 0000000..3846ae2 --- /dev/null +++ b/modules/nf-core/svync/meta.yml @@ -0,0 +1,59 @@ +--- +name: "svync" +description: A tool to standardize VCF files from structural variant callers +keywords: + - structural variants + - vcf + - standardization + - standardize + - sv +tools: + - "svync": + description: "A tool to standardize VCF files from structural variant callers" + homepage: "https://github.com/nvnieuwk/svync" + documentation: "https://github.com/nvnieuwk/svync" + tool_dev_url: "https://github.com/nvnieuwk/svync" + licence: ["MIT"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - vcf: + type: file + description: The input VCF file containing structural variants + pattern: "*.{vcf,vcf.gz}" + - tbi: + type: file + description: The index of the input VCF file containing structural variants + pattern: "*.tbi" + - meta2: + type: map + description: | + Groovy Map containing config meta information + - config: + type: file + description: The config stating how the standardization should happen + pattern: "*.{yml,yaml}" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: The standardized VCF file + pattern: "*.vcf.gz" + +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/modules/nf-core/svync/tests/main.nf.test b/modules/nf-core/svync/tests/main.nf.test new file mode 100644 index 0000000..cc618f9 --- /dev/null +++ b/modules/nf-core/svync/tests/main.nf.test @@ -0,0 +1,84 @@ +nextflow_process { + + name "Test Process SVYNC" + script "../main.nf" + process "SVYNC" + + tag "modules" + tag "modules_nfcore" + tag "svync" + + test("sarscov2 - vcf, config") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['simulated_sv'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv_tbi'], checkIfExists: true) + ] + + input[1] = Channel.of([ + "config.yaml", + "info:", + " SVMETHOD:", + " value: svync", + " description: Type of approach used to detect SV", + " number: 1", + " type: string", + "format:", + " GT:", + " value: 1/1", + " description: Genotype", + " number: 1", + " type: string" + ]) + .collectFile(newLine:true, sort:'index') + .map { [[], it] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf[0][0], + path(process.out.vcf[0][1]).linesGzip[20..30] + ).match() } + ) + } + + } + + test("sarscov2 - vcf, config - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['simulated_sv'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv_tbi'], checkIfExists: true) + ] + + input[1] = Channel.of(["config.yaml", "info:", " SVMETHOD:", " value: svync", " description: Type of approach used to detect SV", " number: 1", " type: string"]) + .collectFile(newLine:true, sort:'index') + .map { [[], it] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/svync/tests/main.nf.test.snap b/modules/nf-core/svync/tests/main.nf.test.snap new file mode 100644 index 0000000..a951b8a --- /dev/null +++ b/modules/nf-core/svync/tests/main.nf.test.snap @@ -0,0 +1,55 @@ +{ + "sarscov2 - vcf, config": { + "content": [ + { + "id": "test", + "single_end": false + }, + [ + "chr21\t6279548\t_2\tN\t\t.\tLowQual\tEND=6280162;IMPRECISE;SVLEN=614;SVMETHOD=svync;SVTYPE=DUP\tGT\t1/1", + "chr21\t6279548\t_3\tN\t\t.\tLowQual\tEND=6280162;IMPRECISE;SVLEN=614;SVMETHOD=svync;SVTYPE=INV\tGT\t1/1", + "chr21\t6497225\t_4\tN\t\t.\tLowQual\tEND=6497978;IMPRECISE;SVLEN=753;SVMETHOD=svync;SVTYPE=INV\tGT\t1/1", + "chr21\t6801798\t_5\tN\t\t.\tLowQual\tEND=6801868;IMPRECISE;SVLEN=70;SVMETHOD=svync;SVTYPE=DEL\tGT\t1/1", + "chr21\t6801868\t_6\tN\t\t.\tLowQual\tEND=6802434;IMPRECISE;SVLEN=566;SVMETHOD=svync;SVTYPE=INV\tGT\t1/1", + "chr21\t6802434\t_7\tN\t\t.\tLowQual\tEND=6802504;IMPRECISE;SVLEN=70;SVMETHOD=svync;SVTYPE=DEL\tGT\t1/1", + "chr21\t6907396\t_8\tN\t\t.\tLowQual\tEND=6907463;IMPRECISE;SVLEN=67;SVMETHOD=svync;SVTYPE=DEL\tGT\t1/1", + "chr21\t6907463\t_9\tN\t\t.\tLowQual\tEND=6908007;IMPRECISE;SVLEN=544;SVMETHOD=svync;SVTYPE=INV\tGT\t1/1", + "chr21\t6908007\t_10\tN\t\t.\tLowQual\tEND=6908074;IMPRECISE;SVLEN=67;SVMETHOD=svync;SVTYPE=DEL\tGT\t1/1", + "chr21\t7776662\t_11\tN\t\t.\tLowQual\tEND=7777323;IMPRECISE;SVLEN=661;SVMETHOD=svync;SVTYPE=INV\tGT\t1/1", + "chr21\t8245292\t_12\tN\t\t.\tLowQual\tEND=8245913;IMPRECISE;SVLEN=621;SVMETHOD=svync;SVTYPE=INV\tGT\t1/1" + ] + ], + "timestamp": "2024-01-23T11:22:21.730837098" + }, + "sarscov2 - vcf, config - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + "versions.yml:md5,dd982c7896f22ebaa0ea51d00472c96c" + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,dd982c7896f22ebaa0ea51d00472c96c" + ] + } + ], + "timestamp": "2024-01-24T12:23:01.99066345" + } +} \ No newline at end of file diff --git a/modules/nf-core/svync/tests/tags.yml b/modules/nf-core/svync/tests/tags.yml new file mode 100644 index 0000000..e63467d --- /dev/null +++ b/modules/nf-core/svync/tests/tags.yml @@ -0,0 +1,2 @@ +svync: + - "modules/nf-core/svync/**" diff --git a/modules/nf-core/tabix/bgzip/environment.yml b/modules/nf-core/tabix/bgzip/environment.yml new file mode 100644 index 0000000..4fe40c5 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/environment.yml @@ -0,0 +1,7 @@ +name: tabix_bgzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf new file mode 100644 index 0000000..8e22f38 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/main.nf @@ -0,0 +1,54 @@ +process TABIX_BGZIP { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta),val(meta2), path(input) + + output: + tuple val(meta),val(meta2), path("${output}") , emit: output + tuple val(meta),val(meta2), path("${output}.gzi"), emit: gzi, optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension()) + extension = in_bgzip ? input.getBaseName().tokenize(".")[-1] : input.getExtension() + output = in_bgzip ? "${prefix}.${extension}" : "${prefix}.${extension}.gz" + command = in_bgzip ? '-d' : '' + // Name the index according to $prefix, unless a name has been requested + if ((args.matches("(^| )-i\\b") || args.matches("(^| )--index(\$| )")) && !args.matches("(^| )-I\\b") && !args.matches("(^| )--index-name\\b")) { + args = args + " -I ${output}.gzi" + } + """ + bgzip $command -c $args -@${task.cpus} $input > ${output} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + in_bgzip = ["gz", "bgz", "bgzf"].contains(input.getExtension()) + output = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz" + + """ + touch ${output} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tabix/bgzip/meta.yml b/modules/nf-core/tabix/bgzip/meta.yml new file mode 100644 index 0000000..621d49e --- /dev/null +++ b/modules/nf-core/tabix/bgzip/meta.yml @@ -0,0 +1,52 @@ +name: tabix_bgzip +description: Compresses/decompresses files +keywords: + - compress + - decompress + - bgzip + - tabix +tools: + - bgzip: + description: | + Bgzip compresses or decompresses files in a similar manner to, and compatible with, gzip. + homepage: https://www.htslib.org/doc/tabix.html + documentation: http://www.htslib.org/doc/bgzip.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input: + type: file + description: file to compress or to decompress +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - output: + type: file + description: Output compressed/decompressed file + pattern: "*." + - gzi: + type: file + description: Optional gzip index file for compressed inputs + pattern: "*.gzi" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" + - "@nvnieuwk" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" + - "@nvnieuwk" diff --git a/modules/nf-core/tabix/bgziptabix/environment.yml b/modules/nf-core/tabix/bgziptabix/environment.yml new file mode 100644 index 0000000..028461c --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/environment.yml @@ -0,0 +1,7 @@ +name: tabix_bgziptabix +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf new file mode 100644 index 0000000..3a099b8 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/main.nf @@ -0,0 +1,47 @@ +process TABIX_BGZIPTABIX { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta),val(meta2), path(input) + + output: + tuple val(meta),val(meta2), path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi + tuple val(meta),val(meta2), path("*.gz"), path("*.csi"), optional: true, emit: gz_csi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + bgzip --threads ${task.cpus} -c $args $input > ${prefix}.${input.getExtension()}.gz + tabix $args2 ${prefix}.${input.getExtension()}.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.${input.getExtension()}.gz + touch ${prefix}.${input.getExtension()}.gz.tbi + touch ${prefix}.${input.getExtension()}.gz.csi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tabix/bgziptabix/meta.yml b/modules/nf-core/tabix/bgziptabix/meta.yml new file mode 100644 index 0000000..438aba4 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/meta.yml @@ -0,0 +1,53 @@ +name: tabix_bgziptabix +description: bgzip a sorted tab-delimited genome file and then create tabix index +keywords: + - bgzip + - compress + - index + - tabix + - vcf +tools: + - tabix: + description: Generic indexer for TAB-delimited genome position files. + homepage: https://www.htslib.org/doc/tabix.html + documentation: https://www.htslib.org/doc/tabix.1.html + doi: 10.1093/bioinformatics/btq671 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tab: + type: file + description: TAB-delimited genome position file + pattern: "*.{bed,gff,sam,vcf}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - gz: + type: file + description: Output compressed file + pattern: "*.{gz}" + - tbi: + type: file + description: tabix index file + pattern: "*.{gz.tbi}" + - csi: + type: file + description: tabix alternate index file + pattern: "*.{gz.csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@maxulysse" + - "@DLBPointon" +maintainers: + - "@maxulysse" + - "@DLBPointon" diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml new file mode 100644 index 0000000..7167fb8 --- /dev/null +++ b/modules/nf-core/tabix/tabix/environment.yml @@ -0,0 +1,7 @@ +name: tabix_tabix +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf new file mode 100644 index 0000000..3c6dea7 --- /dev/null +++ b/modules/nf-core/tabix/tabix/main.nf @@ -0,0 +1,42 @@ +process TABIX_TABIX { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta),val(meta2), path(tab) + + output: + tuple val(meta),val(meta2), path("*.tbi"), optional:true, emit: tbi + tuple val(meta),val(meta2), path("*.csi"), optional:true, emit: csi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + """ + tabix $args $tab + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${tab}.tbi + cat <<-END_VERSIONS > versions.yml + + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/tabix/tabix/meta.yml b/modules/nf-core/tabix/tabix/meta.yml new file mode 100644 index 0000000..ae5b4f4 --- /dev/null +++ b/modules/nf-core/tabix/tabix/meta.yml @@ -0,0 +1,49 @@ +name: tabix_tabix +description: create tabix index from a sorted bgzip tab-delimited genome file +keywords: + - index + - tabix + - vcf +tools: + - tabix: + description: Generic indexer for TAB-delimited genome position files. + homepage: https://www.htslib.org/doc/tabix.html + documentation: https://www.htslib.org/doc/tabix.1.html + doi: 10.1093/bioinformatics/btq671 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tab: + type: file + description: TAB-delimited genome position file compressed with bgzip + pattern: "*.{bed.gz,gff.gz,sam.gz,vcf.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - tbi: + type: file + description: tabix index file + pattern: "*.{tbi}" + - csi: + type: file + description: coordinate sorted index file + pattern: "*.{csi}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" +maintainers: + - "@joseespinosa" + - "@drpatelh" + - "@maxulysse" diff --git a/modules/nf-core/truvari/bench/environment.yml b/modules/nf-core/truvari/bench/environment.yml new file mode 100644 index 0000000..033354b --- /dev/null +++ b/modules/nf-core/truvari/bench/environment.yml @@ -0,0 +1,7 @@ +name: truvari_bench +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::truvari=4.1.0 diff --git a/modules/nf-core/truvari/bench/main.nf b/modules/nf-core/truvari/bench/main.nf new file mode 100644 index 0000000..b0831d0 --- /dev/null +++ b/modules/nf-core/truvari/bench/main.nf @@ -0,0 +1,61 @@ +process TRUVARI_BENCH { + tag "$meta.id $meta2.caller" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/truvari:4.1.0--pyhdfd78af_0': + 'quay.io/biocontainers/truvari:4.1.0--pyhdfd78af_0' }" + + input: + tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple path(fasta), path(fai) + + output: + tuple val(meta), path("*.fn.vcf.gz") , emit: fn_vcf + tuple val(meta), path("*.fn.vcf.gz.tbi") , emit: fn_tbi + tuple val(meta), path("*.fp.vcf.gz") , emit: fp_vcf + tuple val(meta), path("*.fp.vcf.gz.tbi") , emit: fp_tbi + tuple val(meta), path("*.tp-base.vcf.gz") , emit: tp_base_vcf + tuple val(meta), path("*.tp-base.vcf.gz.tbi") , emit: tp_base_tbi + tuple val(meta), path("*.tp-comp.vcf.gz") , emit: tp_comp_vcf + tuple val(meta), path("*.tp-comp.vcf.gz.tbi") , emit: tp_comp_tbi + tuple val(meta), path("*.summary.json") , emit: summary + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions = bed ? "--includebed $bed" : "" + def convert_type = params.dup_to_ins ? "--dup-to-ins" : "" + + """ + truvari bench \\ + --base ${truth_vcf} \\ + --comp ${vcf} \\ + --reference ${fasta} \\ + --output ${prefix} \\ + --pctseq $params.similarity \\ + $convert_type \\ + ${regions} \\ + ${args} + + mv ${prefix}/fn.vcf.gz ./${prefix}.fn.vcf.gz + mv ${prefix}/fn.vcf.gz.tbi ./${prefix}.fn.vcf.gz.tbi + mv ${prefix}/fp.vcf.gz ./${prefix}.fp.vcf.gz + mv ${prefix}/fp.vcf.gz.tbi ./${prefix}.fp.vcf.gz.tbi + mv ${prefix}/tp-base.vcf.gz ./${prefix}.tp-base.vcf.gz + mv ${prefix}/tp-base.vcf.gz.tbi ./${prefix}.tp-base.vcf.gz.tbi + mv ${prefix}/tp-comp.vcf.gz ./${prefix}.tp-comp.vcf.gz + mv ${prefix}/tp-comp.vcf.gz.tbi ./${prefix}.tp-comp.vcf.gz.tbi + mv ${prefix}/summary.json ./${prefix}.summary.json + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + truvari: \$(echo \$(truvari version 2>&1) | sed 's/^Truvari v//' )) + END_VERSIONS + """ +} diff --git a/modules/nf-core/truvari/bench/meta.yml b/modules/nf-core/truvari/bench/meta.yml new file mode 100644 index 0000000..78f9abf --- /dev/null +++ b/modules/nf-core/truvari/bench/meta.yml @@ -0,0 +1,111 @@ +--- +name: "truvari_bench" +description: Given baseline and comparison sets of variants, calculate the recall/precision/f-measure +keywords: + - structural variants + - sv + - vcf + - benchmark + - comparison +tools: + - "truvari": + description: "Structural variant comparison tool for VCFs" + homepage: "https://github.com/ACEnglish/truvari" + documentation: "https://github.com/acenglish/truvari/wiki" + tool_dev_url: "https://github.com/ACEnglish/truvari" + doi: "10.1186/s13059-022-02840-6" + licence: "['MIT']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - vcf: + type: file + description: Input SV VCF file + pattern: "*.vcf.gz" + - tbi: + type: file + description: Input SV VCF index file + pattern: "*.vcf.gz.tbi" + - truth_vcf: + type: file + description: Input VCF file with truth SVs + pattern: "*.vcf.gz" + - tbi: + type: file + description: Input VCF index file with truth SVs + pattern: "*.vcf.gz.tbi" + - bed: + type: file + description: BED file containing regions to compare + pattern: "*.bed" + - meta2: + type: map + description: | + Groovy Map containing fasta information + e.g. `[ id:'test', single_end:false ]` + - fasta: + type: file + description: Reference FASTA file + pattern: "*.{fasta,fa,fna}" + - meta3: + type: map + description: | + Groovy Map containing fasta index information + e.g. `[ id:'test', single_end:false ]` + - fai: + type: file + description: Reference FASTA index file + pattern: "*.fai" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - fn_vcf: + type: file + description: VCF file with false negatives + pattern: "*.fn.vcf.gz" + - fn_tbi: + type: file + description: VCF index file with false negatives + pattern: "*.fn.vcf.gz.tbi" + - fp_vcf: + type: file + description: VCF file with false positives + pattern: "*.fp.vcf.gz" + - fp_tbi: + type: file + description: VCF index file with false positives + pattern: "*.fp.vcf.gz.tbi" + - tp_base_vcf: + type: file + description: VCF file with base true positives + pattern: "*.tp-base.vcf.gz" + - tp_base_tbi: + type: file + description: VCF index file with base true positives + pattern: "*.tp-base.vcf.gz.tbi" + - tp_comp_vcf: + type: file + description: VCF file with compared true positives + pattern: "*.tp-comp.vcf.gz" + - tp_comp_tbi: + type: file + description: VCF index file with compared true positives + pattern: "*.tp-comp.vcf.gz.tbi" + - summary: + type: file + description: Summary JSON file with results from the benchmark + pattern: "*.summary.json" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/nextflow.config b/nextflow.config index b46c50e..73dc5cf 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/variantbenchmarking Nextflow config file + nf-core/svbench Nextflow config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Default config options for all compute environments ---------------------------------------------------------------------------------------- @@ -12,11 +12,42 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options input = null + output = "results" + + // Preprocess spesific parameters + // normalization includes braking down multiallelic samples -m any + // deduplication removes one of the variants in the same position + preprocess = "" + standardization = "" + + // Benchmarking method + method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist + + // minsize effects both truvari and svbenchmark in different ways! svbechmark filters both base and comp calls + // although with truvari it is possible to filter only base, which makes more sense to me! + // I will use this parameter to filter SVs before benchmarking! + min_sv_size = 50 + + // Truvari params + dup_to_ins = "" // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 + + // Manta params + bnd_to_inv = "" // manta reports INV as BND + + // Gridss params + gridss_annotate = "" + + // References genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - + analysis = "germline" + + truth_config_ignore = false + sv_bed = null + snv_bed = null + sdf_file = null // MultiQC options multiqc_config = null @@ -43,7 +74,7 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - + // Max resource options // Defaults only, expecting to be overwritten @@ -70,12 +101,12 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -// Load nf-core/variantbenchmarking custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! +// Load nf-core/svbench custom profiles from different institutions. +// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! // try { -// includeConfig "${params.custom_config_base}/pipeline/variantbenchmarking.config" +// includeConfig "${params.custom_config_base}/pipeline/svbench.config" // } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/variantbenchmarking profiles: ${params.custom_config_base}/pipeline/variantbenchmarking.config") +// System.err.println("WARNING: Could not load nf-core/config/svbench profiles: ${params.custom_config_base}/pipeline/svbench.config") // } profiles { debug { @@ -91,7 +122,6 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false - channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { @@ -106,16 +136,16 @@ profiles { } docker { docker.enabled = true + docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true @@ -169,23 +199,27 @@ profiles { executor.cpus = 4 executor.memory = 8.GB } - test { includeConfig 'conf/test.config' } + test_hg37 { includeConfig 'conf/test_hg37.config' } + test_hg38 { includeConfig 'conf/test_hg38.config' } test_full { includeConfig 'conf/test_full.config' } + truth { includeConfig 'conf/truth.config' } + } // Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile // Will not be used unless Apptainer / Docker / Podman / Singularity are enabled // Set to your registry if you have a mirror of containers -apptainer.registry = 'quay.io' -docker.registry = 'quay.io' -podman.registry = 'quay.io' -singularity.registry = 'quay.io' +apptainer.registry = '' +docker.registry = '' +podman.registry = '' +singularity.registry = '' // Nextflow plugins plugins { - id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet } + // Load igenomes.config if required if (!params.igenomes_ignore) { includeConfig 'conf/igenomes.config' @@ -228,10 +262,10 @@ dag { } manifest { - name = 'nf-core/variantbenchmarking' - author = """Kübra Narcı""" - homePage = 'https://github.com/nf-core/variantbenchmarking' - description = """Nextflow variant benchmarking pipeline""" + name = 'nf-core/svbench' + author = """kuebra.narci@dkfz.de""" + homePage = 'https://github.com/nf-core/svbench' + description = """Master benchmarking pipeline for Structural Variant callers """ mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '1.0dev' diff --git a/nextflow_schema.json b/nextflow_schema.json index 861d2ab..fbbe68f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,288 +1,296 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", - "title": "nf-core/variantbenchmarking pipeline parameters", - "description": "Nextflow variant benchmarking pipeline", - "type": "object", - "definitions": { - "input_output_options": { - "title": "Input/output options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], - "properties": { - "input": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/csv", - "pattern": "^\\S+\\.csv$", - "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/variantbenchmarking/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" - }, - "outdir": { - "type": "string", - "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" - }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" - } - } + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/svbench/master/nextflow_schema.json", + "title": "nf-core/svbench pipeline parameters", + "description": "Master benchmarking pipeline for variant callers ", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["input", "outdir"], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/csv", + "schema": "assets/schema_input.json", + "pattern": "^\\S+\\.(csv|tsv|yaml)$", + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/svbench/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." - }, - "fasta": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code" - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - } - } + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" }, - "institutional_config_options": { - "title": "Institutional config options", - "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", - "properties": { - "custom_config_version": { - "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "custom_config_base": { - "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", - "fa_icon": "fas fa-users-cog" - }, - "config_profile_name": { - "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_description": { - "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - } - } + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + }, + "fasta": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", + "description": "Path to FASTA genome file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" }, - "generic_options": { - "title": "Generic options", - "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", - "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "version": { - "type": "boolean", - "description": "Display version and exit.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "publish_dir_mode": { - "type": "string", - "default": "copy", - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", - "hidden": true - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true - }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true - }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true - }, - "hook_url": { - "type": "string", - "description": "Incoming hook URL for messaging service", - "fa_icon": "fas fa-people-group", - "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", - "hidden": true - }, - "multiqc_config": { - "type": "string", - "format": "file-path", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "validationShowHiddenParams": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "validationFailUnrecognisedParams": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters fails when an unrecognised parameter is found.", - "hidden": true, - "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." - }, - "validationLenientMode": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters in lenient more.", - "hidden": true, - "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." - } - } + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } + } }, - "allOf": [ - { - "$ref": "#/definitions/input_output_options" + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true }, - { - "$ref": "#/definitions/reference_genome_options" + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true }, - { - "$ref": "#/definitions/institutional_config_options" + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], + "hidden": true }, - { - "$ref": "#/definitions/max_job_request_options" + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true }, - { - "$ref": "#/definitions/generic_options" + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "format": "file-path", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "validationShowHiddenParams": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "validationFailUnrecognisedParams": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters fails when an unrecognised parameter is found.", + "hidden": true, + "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." + }, + "validationLenientMode": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters in lenient more.", + "hidden": true, + "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." } - ] + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/reference_genome_options" + }, + { + "$ref": "#/definitions/institutional_config_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] } diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c..0d62beb 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,13 +1,10 @@ -# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. +# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.ruff] +[tool.black] line-length = 120 -target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] -cache-dir = "~/.cache/ruff" +target_version = ["py37", "py38", "py39", "py310"] -[tool.ruff.isort] -known-first-party = ["nf_core"] - -[tool.ruff.per-file-ignores] -"__init__.py" = ["E402", "F401"] +[tool.isort] +profile = "black" +known_first_party = ["nf_core"] +multi_line_output = 3 diff --git a/subworkflows/local/germline_benchmark.nf b/subworkflows/local/germline_benchmark.nf new file mode 100644 index 0000000..e07f1a6 --- /dev/null +++ b/subworkflows/local/germline_benchmark.nf @@ -0,0 +1,103 @@ +// +// GERMLINE: SUBWORKFLOW FOR GERMLINE VARIANTS +// + +params.options = [:] + +include { TRUVARI_PHAB } from '../../modules/local/truvari_phab' addParams( options: params.options ) +include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' addParams( options: params.options ) +include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' addParams( options: params.options ) +include { WITTYER } from '../../modules/local/wittyer' addParams( options: params.options ) +include { VCFDIST } from '../../modules/local/vcfdist' addParams( options: params.options ) +include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon_evaluator' addParams( options: params.options ) + +workflow GERMLINE_BENCHMARK { + take: + input_ch // channel: [val(meta),val(meta2), test_vcf, test_index , truth_vcf, truth_index] + ref // reference channel [ref.fa, ref.fa.fai] + truth_vcf // channel: [val(meta),val(meta2),truth_vcf, truth_index] + + main: + + versions=Channel.empty() + + // SV benchmarking + + if (params.method.contains('truvari')){ + + if(params.harmonize){ + // + // TRUVARI: TRUVARI_PHAB + // + TRUVARI_PHAB( + input_ch, + ref + ) + } + // + // MODULE: TRUVARI_BENCH + // + TRUVARI_BENCH( + input_ch, + ref + ) + versions = versions.mix(TRUVARI_BENCH.out.versions) + + } + + if (params.method.contains('svanalyzer')){ + // + // MODULE: SVANALYZER_SVBENCHMARK + // + // slower than truvari + SVANALYZER_SVBENCHMARK( + input_ch, + ref + ) + versions = versions.mix(SVANALYZER_SVBENCHMARK.out.versions) + + } + + if (params.method.contains('wittyer')){ + // + // MODULE: WITTYER + // + // BIG Advantage: reports by variant type + // Able to report CNV + WITTYER( + input_ch, + [] + ) + versions = versions.mix(WITTYER.out.versions) + } + + + if (params.method.contains('vcfdist')){ + // + // MODULE: VCFDIST + // + VCFDIST( + input_ch, + ref + ) + versions = versions.mix(VCFDIST.out.versions) + } + + if (params.method.contains('bamsurgeon')){ + // + // MODULE: BAMSURGEON_EVALUATOR + // + //https://github.com/adamewing/bamsurgeon/blob/master/scripts/evaluator.py + BAMSURGEON_EVALUATOR( + input_ch.map{it -> tuple(it[0],it[1], it[2], it[3], it[4], it[5])}, + ref, + "SV" + ) + versions = versions.mix(BAMSURGEON_EVALUATOR.out.versions) + } + + + + emit: + versions +} diff --git a/subworkflows/local/germline_benchmark_backup.nf b/subworkflows/local/germline_benchmark_backup.nf new file mode 100644 index 0000000..59607f8 --- /dev/null +++ b/subworkflows/local/germline_benchmark_backup.nf @@ -0,0 +1,162 @@ +// +// GERMLINE: SUBWORKFLOW FOR GERMLINE VARIANTS +// + +params.options = [:] + +include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' addParams( options: params.options ) +include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' addParams( options: params.options ) +include { WITTYER } from '../../modules/local/wittyer' addParams( options: params.options ) +include { VCFDIST } from '../../modules/local/vcfdist' addParams( options: params.options ) +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_QUERY } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_TRUTH } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { ADDHEAD as ADDHEAD_TRUTH } from '../../modules/local/addhead' addParams( options: params.options ) +include { ADDHEAD as ADDHEAD_QUERY } from '../../modules/local/addhead' addParams( options: params.options ) +include { BCFTOOLS_ISEC as BCFTOOLS_ISEC_TRUTH } from '../../modules/nf-core/bcftools/isec' addParams( options: params.options ) +include { BCFTOOLS_ISEC as BCFTOOLS_ISEC_QUERY } from '../../modules/nf-core/bcftools/isec' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) + +workflow GERMLINE_BENCHMARK { + take: + input_ch // channel: [val(meta),val(meta2), test_vcf, test_index , truth_vcf, truth_index] + bed // channel: bed + ref // reference channel [ref.fa, ref.fa.fai] + truth_vcf // channel: [val(meta),val(meta2),truth_vcf, truth_index] + + main: + + versions=Channel.empty() + + // SV benchmarking + + if (params.method.contains('truvari')){ + // + // MODULE: TRUVARI_BENCH + // + TRUVARI_BENCH( + input_ch, + bed, + ref + ) + versions = versions.mix(TRUVARI_BENCH.out.versions) + } + + if (params.method.contains('svanalyzer')){ + // + // MODULE: SVANALYZER_SVBENCHMARK + // + // slower than truvari + SVANALYZER_SVBENCHMARK( + input_ch, + ref, + bed + ) + versions = versions.mix(SVANALYZER_SVBENCHMARK.out.versions) + + // get the original headers from the vcfs + // + // MODULE: BCFTOOLS_VIEW + // + BCFTOOLS_VIEW_QUERY( + input_ch.map{it -> tuple(it[0],it[1], it[2], it[3])} + ) + query_header = BCFTOOLS_VIEW_QUERY.out.header + + BCFTOOLS_VIEW_TRUTH( + truth_vcf + ) + truth_header = BCFTOOLS_VIEW_TRUTH.out.header + versions = versions.mix(BCFTOOLS_VIEW_QUERY.out.versions) + // + // MODULE: BCFTOOLS_REHEADER + // + SVANALYZER_SVBENCHMARK.out.fns.combine(truth_header, by:0) + .map{it -> tuple(it[0],it[1], it[2], it[4])} + .set{fns_header} + fns_header.view() + ADDHEAD_TRUTH( + fns_header + ) + + TABIX_TABIX_1( + ADDHEAD_TRUTH.out.vcf + ) + + ADDHEAD_TRUTH.out.vcf.join(TABIX_TABIX_1.out.tbi, by:1) + .map{it -> tuple( it[1], it[0], it[2], it[4])} + .set{fns_vcf} + + ////// + SVANALYZER_SVBENCHMARK.out.fps.combine(query_header, by:0) + .map{it -> tuple(it[0],it[1], it[2], it[4])} + .set{fps_header} + + ADDHEAD_QUERY( + fps_header + ) + versions = versions.mix(ADDHEAD_QUERY.out.versions) + + TABIX_TABIX_2( + ADDHEAD_QUERY.out.vcf + ) + + ADDHEAD_QUERY.out.vcf.join(TABIX_TABIX_2.out.tbi, by:1) + .map{it -> tuple( it[1], it[0], it[2], it[4])} + .set{fps_ch} + // + // MODULE: BCFTOOLS_ISEC + // + + // Find TP_comp (query) + input_ch.map{it -> tuple(it[0],it[1], it[2], it[3])} + .combine(fps_ch, by:0) + .map{it -> tuple(it[0],it[1], it[2], it[5], it[3], it[6])} + .set{query_ch} + + BCFTOOLS_ISEC_QUERY( + query_ch + ) + + // Find TP_comp (query) + truth_vcf.combine(fns_vcf, by:0) + .map{it -> tuple(it[0],it[1], it[2], it[5], it[3], it[6])} + .set{truth_ch} + + // Find TP_base (truth) + BCFTOOLS_ISEC_TRUTH( + truth_ch + ) + versions = versions.mix(BCFTOOLS_ISEC_TRUTH.out.versions) + + } + + if (params.method.contains('wittyer')){ + // + // MODULE: WITTYER + // + WITTYER( + input_ch, + bed, + [] + ) + versions = versions.mix(WITTYER.out.versions) + } + + + if (params.method.contains('vcfdist')){ + // + // MODULE: VCFDIST + // + VCFDIST( + input_ch, + ref, + bed + ) + versions = versions.mix(VCFDIST.out.versions) + } + + + emit: + versions +} diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 0aecf87..2c227a8 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -2,43 +2,55 @@ // Check input samplesheet and get read channels // -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' +params.options = [:] + +include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' addParams( options: params.options ) workflow INPUT_CHECK { take: samplesheet // file: /path/to/samplesheet.csv main: - SAMPLESHEET_CHECK ( samplesheet ) + SAMPLESHEET_CHECK (samplesheet) .csv .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } + .map{ create_vcf_channel(it) } + .set {ch_sample} emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] + ch_sample // channel: [ val(meta), test_vcf] + versions = SAMPLESHEET_CHECK.out.versions } -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map +def create_vcf_channel(LinkedHashMap row) { +// create meta map def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() + meta.id = params.sample + def meta2 = [:] + meta2.caller = row.caller // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" + def vcf_meta = [] + if (!file(row.test_vcf).exists()) { + exit 1, "ERROR: Please check input samplesheet -> Test file does not exist!\n${row.test_vcf}" + } + + if (meta2.caller == "delly"){ + vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/delly.yaml")] + } + else if (meta2.caller == "gridss"){ + vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/gridss.yaml")] + } + else if (meta2.caller == "manta"){ + if (file("${projectDir}/assets/svync/manta.yaml").exists()){ + vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/manta.yaml")] + } } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta + else if (meta2.caller == "smoove"){ + vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/smoove.yaml")] + } + else{ + vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/default.yaml")] + } + return vcf_meta } diff --git a/subworkflows/local/prepare_regions.nf b/subworkflows/local/prepare_regions.nf new file mode 100644 index 0000000..050cf10 --- /dev/null +++ b/subworkflows/local/prepare_regions.nf @@ -0,0 +1,44 @@ +// +// PREPARE_STRATIFICATIONS: SUBWORKFLOW TO PREPARE BED FILES - HIGH CONFIDENCE AND OTHER LEVELS +// + +params.options = [:] + +include { MAIN_CHROMS } from '../../modules/local/main_chroms.nf' addParams( options: params.options ) +include { EXTRACT_MAIN } from '../../modules/local/extract_main.nf' addParams( options: params.options ) + + +workflow PREPARE_REGIONS { + take: + ref // reference channel [ref.fa, ref.fa.fai] + high_conf + + main: + + versions=Channel.empty() + + ref.map { it -> tuple([id: it[0].baseName], it[1]) } + .set{fasta} + + // this is not working! + + // get contig file including only main chroms + MAIN_CHROMS( + fasta + ) + main_chroms = MAIN_CHROMS.out.sizes + versions = versions.mix(MAIN_CHROMS.out.versions) + + high_conf.map { it -> tuple([id: it[0].baseName], it[0]) } + .set{bed} + bed.view() + EXTRACT_MAIN( + bed + ) + chr_list = EXTRACT_MAIN.out.chr_list + + emit: + main_chroms + chr_list + versions +} diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf new file mode 100644 index 0000000..f01ef6e --- /dev/null +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -0,0 +1,131 @@ +// +// PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS +// + +params.options = [:] + +include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) +include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_1 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_2 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_3 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TEST } from '../../modules/nf-core/bcftools/reheader' addParams( options: params.options ) + + +workflow PREPARE_VCFS_TEST { + take: + input_ch // channel: [val(meta),val(meta2), vcf] + ref // reference channel [ref.fa, ref.fa.fai] + main_chroms // channel path(chrom sizes) + chr_list + + main: + + versions=Channel.empty() + + //ref.map { it -> tuple([id: it[0].baseName], it[1]) } + // .set{fasta} + // + // PREPARE_VCFS + // + // Reheader needed to standardize sample names + BCFTOOLS_REHEADER_TEST( + input_ch, + ref + ) + versions = versions.mix(BCFTOOLS_REHEADER_TEST.out.versions) + + TABIX_BGZIPTABIX_1( + BCFTOOLS_REHEADER_TEST.out.vcf + ) + vcf_ch = TABIX_BGZIPTABIX_1.out.gz_tbi + + // + // BCFTOOLS_VIEW + // + // To filter out contigs! + BCFTOOLS_VIEW( + vcf_ch + ) + versions = versions.mix(BCFTOOLS_VIEW.out.versions) + + TABIX_BGZIPTABIX_2( + BCFTOOLS_VIEW.out.vcf + ) + vcf_ch = TABIX_BGZIPTABIX_2.out.gz_tbi + + if (params.preprocess.contains("normalization")){ + // + // BCFTOOLS_NORM + // + // Breaks down -any- multi-allelic variants + BCFTOOLS_NORM_1( + vcf_ch, + ref, + [[],[]] + ) + versions = versions.mix(BCFTOOLS_NORM_1.out.versions) + + TABIX_TABIX_1( + BCFTOOLS_NORM_1.out.vcf + ) + + BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:1) + .map{it -> tuple( it[1], it[0], it[2], it[4])} + .set{vcf_ch} + } + if (params.min_sv_size > 0){ + + TABIX_BGZIP( + vcf_ch.map{it -> tuple( it[0], it[1], it[2])} + ) + versions = versions.mix(TABIX_BGZIP.out.versions) + + // + // MODULE: SURVIVOR_FILTER + // + // filters out smaller SVs than min_sv_size + SURVIVOR_FILTER( + TABIX_BGZIP.out.output.map{it -> tuple( it[0], it[1], it[2], [])}, + params.min_sv_size, + -1, + -1, + -1 + ) + versions = versions.mix(SURVIVOR_FILTER.out.versions) + + TABIX_BGZIPTABIX_3( + SURVIVOR_FILTER.out.vcf + ) + vcf_ch = TABIX_BGZIPTABIX_3.out.gz_tbi + } + + if (params.preprocess.contains("deduplication")){ + // + // BCFTOOLS_NORM + // + // Deduplicates variants at the same position test + BCFTOOLS_NORM_2( + vcf_ch, + ref, + [[],[]] + ) + versions = versions.mix(BCFTOOLS_NORM_2.out.versions) + + TABIX_TABIX_2( + BCFTOOLS_NORM_2.out.vcf + ) + + BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:1) + .map{it -> tuple( it[1], it[0], it[2], it[4])} + .set{vcf_ch} + } + emit: + vcf_ch + versions +} diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf new file mode 100644 index 0000000..10cff46 --- /dev/null +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -0,0 +1,99 @@ +// +// PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS +// + +params.options = [:] + +include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix.nf' addParams( options: params.options ) +include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_3 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TRUTH } from '../../modules/nf-core/bcftools/reheader' addParams( options: params.options ) + +workflow PREPARE_VCFS_TRUTH { + take: + truth_ch // channel: [val(meta), vcf] + ref // reference channel [ref.fa, ref.fa.fai] + + main: + + versions=Channel.empty() + + // + // PREPARE_VCFS + // + truth_ch.map { it -> tuple([id: params.sample],[caller:"truth"], it[0]) } + .set{truth} + + // BGZIP if needed and index truth + BGZIP_TABIX( + truth + ) + versions = versions.mix(BGZIP_TABIX.out.versions) + vcf_ch = BGZIP_TABIX.out.gz_tbi + + // Reheader needed to standardize sample names + BCFTOOLS_REHEADER_TRUTH( + vcf_ch, + ref + ) + versions = versions.mix(BCFTOOLS_REHEADER_TRUTH.out.versions) + + TABIX_BGZIPTABIX( + BCFTOOLS_REHEADER_TRUTH.out.vcf + ) + vcf_ch = TABIX_BGZIPTABIX.out.gz_tbi + + if (params.preprocess.contains("normalization")){ + // + // MODULE: BCFTOOLS_NORM + // + // Normalize test + // multi-allelic variants will be splitted. + BCFTOOLS_NORM_1( + vcf_ch, + ref, + [[],[]] + ) + versions = versions.mix(BCFTOOLS_NORM_1.out.versions) + + TABIX_TABIX_1( + BCFTOOLS_NORM_1.out.vcf + ) + versions = versions.mix(TABIX_TABIX_1.out.versions) + + BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:1) + .map{it -> tuple(it[1],it[0], it[2], it[4])} + .set{vcf_ch} + } + if (params.preprocess.contains("deduplication")){ + // + // MODULE: BCFTOOLS_NORM + // + // Deduplicate variants at the same position + BCFTOOLS_NORM_2( + vcf_ch, + ref, + [[],[]] + ) + versions = versions.mix(BCFTOOLS_NORM_2.out.versions) + + TABIX_TABIX_2( + BCFTOOLS_NORM_2.out.vcf + ) + versions = versions.mix(TABIX_TABIX_2.out.versions) + + BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:1) + .map{it -> tuple(it[1],it[0], it[2], it[4])} + .set{vcf_ch} + } + + + emit: + vcf_ch + versions +} diff --git a/subworkflows/local/report_vcf_statistics.nf b/subworkflows/local/report_vcf_statistics.nf new file mode 100644 index 0000000..a17a262 --- /dev/null +++ b/subworkflows/local/report_vcf_statistics.nf @@ -0,0 +1,52 @@ +// +// PREPARE_VCFS: SUBWORKFLOW TO REPORT VCF STATS +// + +params.options = [:] + +include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' addParams( options: params.options ) +include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' addParams( options: params.options ) + +workflow REPORT_VCF_STATISTICS { + take: + input_ch // channel: [val(meta), vcf, index] + + main: + + versions=Channel.empty() + + // + // SURVIVOR_STATS + // + + SURVIVOR_STATS( + input_ch, + -1, + -1, + -1 + ) + survivor_stats = SURVIVOR_STATS.out.stats + versions = versions.mix(SURVIVOR_STATS.out.versions) + + // + // BCFTOOLS_STATS + // + BCFTOOLS_STATS( + input_ch, + [[],[]], + [[],[]], + [[],[]], + [[],[]], + [[],[]] + ) + bcftools_stats = BCFTOOLS_STATS.out.stats + versions = versions.mix(BCFTOOLS_STATS.out.versions) + + + // Add here a tool, to visualize SV statistics in a histogram. + + emit: + bcftools_stats + survivor_stats + versions +} diff --git a/subworkflows/local/somatic_benchmark.nf b/subworkflows/local/somatic_benchmark.nf new file mode 100644 index 0000000..cc1bc80 --- /dev/null +++ b/subworkflows/local/somatic_benchmark.nf @@ -0,0 +1,49 @@ +// +// SOMATIC: SUBWORKFLOW FOR SOMATIC VARIANTS +// + +params.options = [:] + +include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' addParams( options: params.options ) +include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' addParams( options: params.options ) + +workflow SOMATIC_BENCHMARK { + take: + input_ch // channel: [val(meta), test_vcf,test_index, truth_vcf, truth_index, bed] + ref // reference channel [ref.fa, ref.fa.fai] + + main: + + versions=Channel.empty() + + // SV Benchmarking + // + // MODULE: TRUVARI_BENCH + // + TRUVARI_BENCH( + input_ch, + ref + ) + versions = versions.mix(TRUVARI_BENCH.out.versions) + + // SV Benchmarking + // + // MODULE: SVANALYZER_SVBENCHMARK + // + // note: slow + //SVANALYZER_SVBENCHMARK( + // bench.sv, + // ref, + // sv_bed + //) + //versions = versions.mix(SVANALYZER_SVBENCHMARK.out.versions) + + // Small Variant Benchmarking + + // SOMPY https://sites.google.com/view/seqc2/home/benchmarking-examples?authuser=0 + // is used for somatic variant benchmarking! + + + emit: + versions +} diff --git a/subworkflows/local/vcf_conversion.nf b/subworkflows/local/vcf_conversion.nf new file mode 100644 index 0000000..b6e355f --- /dev/null +++ b/subworkflows/local/vcf_conversion.nf @@ -0,0 +1,126 @@ +// +// VCF_CONVERSIONS: SUBWORKFLOW TO apply tool spesific conversions +// + +params.options = [:] + +include { MANTA_CONVERTINVERSION } from '../../modules/nf-core/manta/convertinversion' addParams( options: params.options ) +include { GRIDSS_ANNOTATION } from '../../modules/local/gridss_annotation' addParams( options: params.options ) +include { SVYNC } from '../../modules/nf-core/svync' addParams( options: params.options ) +include { AWK_SORT } from '../../modules/local/awk_sort.nf' addParams( options: params.options ) + +workflow VCF_CONVERSIONS { + take: + input_ch // channel: [val(meta),val(meta2), vcf, config.yml] + ref // reference channel [ref.fa, ref.fa.fai] + + main: + + out_vcf_ch = Channel.empty() + versions = Channel.empty() + + // + // MODULE: AWK_SORT + // + // sort and index input test files + + AWK_SORT( + input_ch.map{it -> tuple(it[0], it[1], it[2])} + ) + versions = versions.mix(AWK_SORT.out.versions) + vcf_ch = AWK_SORT.out.vcf + + // + // MODULE: SVYNC + // + // + if(params.standardization){ + + vcf_ch.branch{ + tool: it[1].caller == "delly" || it[1].caller == "gridss" || it[1].caller == "manta" || it[1].caller == "smoove" + other: true} + .set{main_vcf_ch} + + + input_ch.map{it -> tuple(it[0], it[1], it[3])} + .combine(vcf_ch, by:1) + .map{it -> tuple(it[1], it[0], it[4], it[5], it[2])} + .set{snd_ch} + + snd_ch.branch{ + tool: it[1].caller == "delly" || it[1].caller == "gridss" || it[1].caller == "manta" || it[1].caller == "smoove" + other: true} + .set{input} + + SVYNC( + input.tool + ) + out_vcf_ch = out_vcf_ch.mix(SVYNC.out.vcf) + out_vcf_ch = out_vcf_ch.mix(input.other) + }else{ + out_vcf_ch = vcf_ch + } + + out2_vcf_ch = Channel.empty() + + // Check tool spesific conversions + if(params.bnd_to_inv){ + + out_vcf_ch.branch{ + tool: it[1].caller == "manta" || it[1].caller == "dragen" + other: true} + .set{input} + // + // MANTA_CONVERTINVERSION + // + //NOTE: should also work for dragen + // Not working now!!!!! + + MANTA_CONVERTINVERSION( + input.tool, + ref + ) + versions = versions.mix(MANTA_CONVERTINVERSION.out.versions) + + out2_vcf_ch = out2_vcf_ch.mix(MANTA_CONVERTINVERSION.out.vcf_tabi) + out2_vcf_ch = out2_vcf_ch.mix(input.other) + + // https://github.com/srbehera/DRAGEN_Analysis/blob/main/convertInversion.py + + } + else{ + out2_vcf_ch = out_vcf_ch + } + + out3_vcf_ch = Channel.empty() + + if (params.gridss_annotate){ + out2_vcf_ch.branch{ + tool: it[1].caller == "gridss" + other: true} + .set{input} + + // + // GRIDSS_ANNOTATION + // + // https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R#L9 + // GRIDSS simple event annotation + GRIDSS_ANNOTATION( + input.tool, + ref + ) + versions = versions.mix(GRIDSS_ANNOTATION.out.versions) + + out3_vcf_ch = out3_vcf_ch.mix(GRIDSS_ANNOTATION.out.vcf) + out3_vcf_ch = out3_vcf_ch.mix(input.other) + } + else{ + out3_vcf_ch = out2_vcf_ch + } + + // https://github.com/EUCANCan/variant-extractor/blob/main/examples/vcf_to_csv.py + + emit: + out3_vcf_ch + versions +} diff --git a/subworkflows/local/visualizations.nf b/subworkflows/local/visualizations.nf new file mode 100644 index 0000000..29f727c --- /dev/null +++ b/subworkflows/local/visualizations.nf @@ -0,0 +1,35 @@ +// +// PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS +// + +params.options = [:] + +include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix.nf' addParams( options: params.options ) +include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_3 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { BGZIP_TABIX as BGZIP_TABIX_1 } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) +include { BGZIP_TABIX as BGZIP_TABIX_2 } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) + +workflow PREPARE_VCFS_TRUTH { + take: + truth_ch // channel: [val(meta), vcf] + ref // reference channel [ref.fa, ref.fa.fai] + main_chroms // channel: path(chrom sizes) + + main: + + versions=Channel.empty() + +// Check tool spesific conversions + + // https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R#L9 + // GRIDSS simple event annotation + + emit: + vcf_ch + versions +} diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 04a3bf2..62c0b93 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -4,7 +4,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryLog; paramsSummaryMap; fromSamplesheet } from 'plugin/nf-validation' def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' @@ -13,7 +13,22 @@ def summary_params = paramsSummaryMap(workflow) // Print parameter summary log to screen log.info logo + paramsSummaryLog(workflow) + citation -WorkflowVariantbenchmarking.initialise(params, log) +WorkflowBenchmark.initialise(params, log) + +// check mandatory parameters +ref = Channel.fromPath([params.fasta,params.fai], checkIfExists: true).collect() + +// check high confidence files + +truth = params.truth ? Channel.fromPath(params.truth, checkIfExists: true).collect() + : Channel.empty() + +high_conf = params.high_conf ? Channel.fromPath(params.high_conf, checkIfExists: true).collect() + : Channel.empty() + +// TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID + + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -35,7 +50,16 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { SOMATIC_BENCHMARK } from '../subworkflows/local/somatic_benchmark' +include { GERMLINE_BENCHMARK } from '../subworkflows/local/germline_benchmark' +include { PREPARE_REGIONS } from '../subworkflows/local/prepare_regions' +include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' +include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' +include { VCF_CONVERSIONS } from '../subworkflows/local/vcf_conversion' +include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TEST } from '../subworkflows/local/report_vcf_statistics' +include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_vcf_statistics' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -46,7 +70,6 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' @@ -59,7 +82,7 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft // Info required for completion email and summary def multiqc_report = [] -workflow VARIANTBENCHMARKING { +workflow SVBENCH { ch_versions = Channel.empty() @@ -70,17 +93,97 @@ workflow VARIANTBENCHMARKING { file(params.input) ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + ch_input = INPUT_CHECK.out.ch_sample + + // + // PREPARE_REGIONS: prepare stratifications and contigs + // + PREPARE_REGIONS( + ref, + high_conf + ) + ch_versions = ch_versions.mix(PREPARE_REGIONS.out.versions) + + // + // SUBWORKFLOW: VCF_CONVERSIONS + // + // Standardize VCFs, tool spesific modifications + VCF_CONVERSIONS( + ch_input, + ref + ) + ch_versions = ch_versions.mix(VCF_CONVERSIONS.out.versions) + + // + // SUBWORKFLOW: Prepare and normalize input vcfs + // + PREPARE_VCFS_TRUTH( + truth, + ref + ) + ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) + + PREPARE_VCFS_TEST( + VCF_CONVERSIONS.out.out3_vcf_ch.map{it -> tuple(it[0], it[1], it[2], it[3])}, + ref, + PREPARE_REGIONS.out.main_chroms, + PREPARE_REGIONS.out.chr_list + ) + ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) // - // MODULE: Run FastQC + // SUBWORKFLOW: GET STATISTICS OF FILES + // + //REPORT_STATISTICS_TRUTH( // - FASTQC ( - INPUT_CHECK.out.reads + REPORT_STATISTICS_TEST( + PREPARE_VCFS_TEST.out.vcf_ch + ) + REPORT_STATISTICS_TRUTH( + PREPARE_VCFS_TRUTH.out.vcf_ch ) - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) + + // prepare benchmark set + + high_conf.map { it -> tuple([id: params.sample],[caller:"truth"], it[0]) } + .set{bed} + + PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch, by:0) + .combine(bed, by:0) + .map{it -> tuple(it[0],it[1], it[2], it[3], it[5], it[6], it[8])} + .set{bench_ch} + + // + // SUBWORKFLOW: GERMLINE_BENCHMARK + // + //Benchmarking spesific to germline samples + + GERMLINE_BENCHMARK( + bench_ch, + ref, + PREPARE_VCFS_TRUTH.out.vcf_ch + ) + ch_versions = ch_versions.mix(GERMLINE_BENCHMARK.out.versions) + + + if (params.analysis.contains("somatic")){ + + // SOMATIC VARIANT BENCHMARKING + SOMATIC_BENCHMARK( + bench_ch, + ref + ) + ch_versions = ch_versions.mix(SOMATIC_BENCHMARK.out.versions) + } + + // TODO: NEED A TOOL TO COLLECT METRICS AND ROCS LIKE DATAVZRD OR SQLITE DATABASE + + + // TODO: BENCHMARKING OF CNV + + + // TODO: TRIO ANALYSIS : MENDELIAN INCONSISTANCE CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') @@ -89,17 +192,17 @@ workflow VARIANTBENCHMARKING { // // MODULE: MultiQC // - workflow_summary = WorkflowVariantbenchmarking.paramsSummaryMultiqc(workflow, summary_params) + workflow_summary = WorkflowBenchmark.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) - methods_description = WorkflowVariantbenchmarking.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) + methods_description = WorkflowBenchmark.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) ch_methods_description = Channel.value(methods_description) ch_multiqc_files = Channel.empty() ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + //ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) MULTIQC ( ch_multiqc_files.collect(), @@ -127,13 +230,6 @@ workflow.onComplete { } } -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } -} - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From da3f4eaa111ff73bef3ae4d0d8e2a6bab6b26de6 Mon Sep 17 00:00:00 2001 From: maxulysse Date: Fri, 16 Feb 2024 17:13:04 +0100 Subject: [PATCH 002/292] svbench + benchmark -> variantbenchmarking --- CHANGELOG.md | 4 ++-- CITATIONS.md | 2 +- README.md | 28 ++++++++++++------------- assets/adaptivecard.json | 2 +- assets/email_template.html | 16 +++++++------- assets/email_template.txt | 10 ++++----- assets/methods_description_template.yml | 8 +++---- assets/multiqc_config.yml | 8 +++---- assets/schema_input.json | 6 +++--- assets/sendmail_template.txt | 6 +++--- assets/slackreport.json | 2 +- conf/base.config | 2 +- conf/test_full.config | 2 +- conf/test_hg19.config | 2 +- conf/test_hg37.config | 2 +- conf/test_hg38.config | 6 +++--- conf/test_hg38_somatic.config | 2 +- docs/README.md | 4 ++-- docs/output.md | 2 +- docs/usage.md | 12 +++++------ lib/WorkflowMain.groovy | 2 +- lib/WorkflowVariantbenchmarking.groovy | 2 +- main.nf | 18 ++++++++-------- modules.json | 4 ++-- nextflow.config | 18 ++++++++-------- nextflow_schema.json | 6 +++--- workflows/variantbenchmarking.nf | 14 ++++++------- 27 files changed, 95 insertions(+), 95 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8f39c6e..e86fa48 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -1,11 +1,11 @@ -# nf-core/benchmark: Changelog +# nf-core/variantbenchmarking: Changelog The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). ## v1.0dev - [date] -Initial release of nf-core/benchmark, created with the [nf-core](https://nf-co.re/) template. +Initial release of nf-core/variantbenchmarking, created with the [nf-core](https://nf-co.re/) template. ### `Added` diff --git a/CITATIONS.md b/CITATIONS.md index 2bc3e86..28d5f49 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -1,4 +1,4 @@ -# nf-core/benchmark: Citations +# nf-core/variantbenchmarking: Citations ## [nf-core](https://pubmed.ncbi.nlm.nih.gov/32055031/) diff --git a/README.md b/README.md index 090c8b2..56b03b3 100644 --- a/README.md +++ b/README.md @@ -1,19 +1,19 @@ -# ![nf-core/svbench](docs/images/nf-core-svbench_logo_light.png#gh-light-mode-only) +# ![nf-core/variantbenchmarking](docs/images/nf-core-variantbenchmarking_logo_light.png#gh-light-mode-only) -[![GitHub Actions CI Status](https://github.com/nf-core/svbench/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/svbench/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/svbench/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/svbench/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/svbench/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/svbench) +[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/variantbenchmarking) -[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23benchmark-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/svbench)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23benchmark-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) ## Introduction -**nf-core/svbench** is a bioinformatics pipeline that ... +**nf-core/variantbenchmarking** is a bioinformatics pipeline that ... ```bash -nextflow run nf-core/svbench \ +nextflow run nf-core/variantbenchmarking \ -profile \ --input samplesheet.csv \ --outdir @@ -76,17 +76,17 @@ provided by the `-c` Nextflow option can be used to provide any configuration _* see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). ::: -For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/svbench/usage) and the [parameter documentation](https://nf-co.re/svbench/parameters). +For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). ## Pipeline output -To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/svbench/results) tab on the nf-core website pipeline page. +To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page. For more details about the output files and reports, please refer to the -[output documentation](https://nf-co.re/svbench/output). +[output documentation](https://nf-co.re/variantbenchmarking/output). ## Credits -nf-core/svbench was originally written by kuebra.narci@dkfz.de. +nf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de. We thank the following people for their extensive assistance in the development of this pipeline: @@ -96,12 +96,12 @@ We thank the following people for their extensive assistance in the development If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). -For further information or help, don't hesitate to get in touch on the [Slack `#svbench` channel](https://nfcore.slack.com/channels/svbench) (you can join with [this invite](https://nf-co.re/join/slack)). +For further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)). ## Citations - + diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json index 4f89163..4b5f7c5 100644 --- a/assets/adaptivecard.json +++ b/assets/adaptivecard.json @@ -17,7 +17,7 @@ "size": "Large", "weight": "Bolder", "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", - "text": "nf-core/benchmark v${version} - ${runName}", + "text": "nf-core/variantbenchmarking v${version} - ${runName}", "wrap": true }, { diff --git a/assets/email_template.html b/assets/email_template.html index 323a139..3334523 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -6,9 +6,9 @@ - nf-core/benchmark Pipeline Report + nf-core/variantbenchmarking Pipeline Report
-

nf-core/benchmark v${version}

+

nf-core/variantbenchmarking v${version}

Run Name: $runName

<% if (!success){ out << """ @@ -37,7 +37,7 @@

Run Name: $runName

" >

- nf-core/benchmark execution completed unsuccessfully! + nf-core/variantbenchmarking execution completed unsuccessfully!

The exit status of the task that caused the workflow execution to fail @@ -60,7 +60,7 @@

border-radius: 4px; " > - nf-core/benchmark execution completed successfully! + nf-core/variantbenchmarking execution completed successfully!

""" } %> @@ -123,10 +123,10 @@

Pipeline Configuration:

Process Name \\", - " \\ Software Version
CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
yaml5.4.1
TOOL1tool10.11.9
TOOL2tool21.9
WorkflowNextflow
-

nf-core/benchmark

+

nf-core/variantbenchmarking

- https://github.com/nf-core/benchmarkhttps://github.com/nf-core/variantbenchmarking

diff --git a/assets/email_template.txt b/assets/email_template.txt index 4acfb7f..38114da 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,15 +4,15 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/benchmark v${version} + nf-core/variantbenchmarking v${version} ---------------------------------------------------- Run Name: $runName <% if (success){ - out << "## nf-core/benchmark execution completed successfully! ##" + out << "## nf-core/variantbenchmarking execution completed successfully! ##" } else { out << """#################################################### -## nf-core/benchmark execution completed unsuccessfully! ## +## nf-core/variantbenchmarking execution completed unsuccessfully! ## #################################################### The exit status of the task that caused the workflow execution to fail was: $exitStatus. The full error message was: @@ -35,5 +35,5 @@ Pipeline Configuration: <% out << summary.collect{ k,v -> " - $k: $v" }.join("\n") %> -- -nf-core/benchmark -https://github.com/nf-core/benchmark +nf-core/variantbenchmarking +https://github.com/nf-core/variantbenchmarking diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index 35dd7dd..1e3c455 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -1,13 +1,13 @@ -id: "nf-core-benchmark-methods-description" +id: "nf-core-variantbenchmarking-methods-description" description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." -section_name: "nf-core/benchmark Methods Description" -section_href: "https://github.com/nf-core/benchmark" +section_name: "nf-core/variantbenchmarking Methods Description" +section_href: "https://github.com/nf-core/variantbenchmarking" plot_type: "html" ## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

Methods

-

Data was processed using nf-core/benchmark v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

+

Data was processed using nf-core/variantbenchmarking v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

${workflow.commandLine}

${tool_citations}

diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index f195fd3..9653868 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,13 +1,13 @@ report_comment: > - This report has been generated by the nf-core/benchmark + This report has been generated by the nf-core/variantbenchmarking analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: - "nf-core-benchmark-methods-description": + "nf-core-variantbenchmarking-methods-description": order: -1000 software_versions: order: -1001 - "nf-core-benchmark-summary": + "nf-core-variantbenchmarking-summary": order: -1002 export_plots: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 5746c3f..2ef1310 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/benchmark/master/assets/schema_input.json", - "title": "nf-core/benchmark pipeline - params.input schema", + "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/assets/schema_input.json", + "title": "nf-core/variantbenchmarking pipeline - params.input schema", "description": "Schema for the file provided with params.input", "type": "array", "items": { @@ -11,7 +11,7 @@ "type": "string", "pattern": "", "errorMessage": "Test VCF must be provided, cannot contain spaces and must have extension '.vcf.gz'" - }, + }, "caller": { "type": "string", "pattern": "^\\S+$", diff --git a/assets/sendmail_template.txt b/assets/sendmail_template.txt index 22c306e..6ab0eb8 100644 --- a/assets/sendmail_template.txt +++ b/assets/sendmail_template.txt @@ -9,12 +9,12 @@ Content-Type: text/html; charset=utf-8 $email_html --nfcoremimeboundary -Content-Type: image/png;name="nf-core-benchmark_logo.png" +Content-Type: image/png;name="nf-core-variantbenchmarking_logo.png" Content-Transfer-Encoding: base64 Content-ID: -Content-Disposition: inline; filename="nf-core-benchmark_logo_light.png" +Content-Disposition: inline; filename="nf-core-variantbenchmarking_logo_light.png" -<% out << new File("$projectDir/assets/nf-core-benchmark_logo_light.png"). +<% out << new File("$projectDir/assets/nf-core-variantbenchmarking_logo_light.png"). bytes. encodeBase64(). toString(). diff --git a/assets/slackreport.json b/assets/slackreport.json index d2d6f69..9e94a79 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/svbench ${version} - ${runName}", + "author_name": "nf-core/variantbenchmarking ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/base.config b/conf/base.config index a927e41..0e24cb6 100644 --- a/conf/base.config +++ b/conf/base.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/benchmark Nextflow base config file + nf-core/variantbenchmarking Nextflow base config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ A 'blank slate' config file, appropriate for general use on most high performance compute environments. Assumes that all software is installed and available on diff --git a/conf/test_full.config b/conf/test_full.config index 7693ac0..57fba2a 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile test_full, --outdir + nextflow run nf-core/variantbenchmarking -profile test_full, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_hg19.config b/conf/test_hg19.config index 2734e5a..3623854 100644 --- a/conf/test_hg19.config +++ b/conf/test_hg19.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile test, --outdir + nextflow run nf-core/variantbenchmarking -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_hg37.config b/conf/test_hg37.config index 97db262..aa20eb1 100644 --- a/conf/test_hg37.config +++ b/conf/test_hg37.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile test, --outdir + nextflow run nf-core/variantbenchmarking -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_hg38.config b/conf/test_hg38.config index 05958d2..3336ae6 100644 --- a/conf/test_hg38.config +++ b/conf/test_hg38.config @@ -6,7 +6,7 @@ Use as follows: nextflow run nf-core/benchmark -profile test, --outdir - +variantbenchmarking ---------------------------------------------------------------------------------------- */ @@ -31,7 +31,7 @@ params { // Processes analysis = 'germline' //somatic method = 'truvari,svanalyzer,wittyer,vcfdist' // --not working for now : wittyer, vcfdist - //harmonize = true // ATTENTION : takes long time because of multi-alignment process. + //harmonize = true // ATTENTION : takes long time because of multi-alignment process. similarity = 0 // determines the sequence similarity level in benchmarking. preprocess = "normalization, deduplication" @@ -39,7 +39,7 @@ params { //dup_to_ins = true // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 // Manta params - //bnd_to_inv = true // manta reports INV as BND + //bnd_to_inv = true // manta reports INV as BND // Gridss params //gridss_annotate = true diff --git a/conf/test_hg38_somatic.config b/conf/test_hg38_somatic.config index 23e6ecb..e8abecc 100644 --- a/conf/test_hg38_somatic.config +++ b/conf/test_hg38_somatic.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile test, --outdir + nextflow run nf-core/variantbenchmarking -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/docs/README.md b/docs/README.md index 23c4836..c79ccc9 100644 --- a/docs/README.md +++ b/docs/README.md @@ -1,6 +1,6 @@ -# nf-core/benchmark: Documentation +# nf-core/variantbenchmarking: Documentation -The nf-core/benchmark documentation is split into the following pages: +The nf-core/variantbenchmarking documentation is split into the following pages: - [Usage](usage.md) - An overview of how the pipeline works, how to run it and a description of all of the different command-line flags. diff --git a/docs/output.md b/docs/output.md index 34599a9..c671e3f 100644 --- a/docs/output.md +++ b/docs/output.md @@ -1,4 +1,4 @@ -# nf-core/benchmark: Output +# nf-core/variantbenchmarking: Output ## Introduction diff --git a/docs/usage.md b/docs/usage.md index 365ca0c..3093473 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -1,6 +1,6 @@ -# nf-core/benchmark: Usage +# nf-core/variantbenchmarking: Usage -## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/benchmark/usage](https://nf-co.re/benchmark/usage) +## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/variantbenchmarking/usage](https://nf-co.re/variantbenchmarking/usage) > _Documentation of pipeline parameters is generated automatically from the pipeline schema and can no longer be found in markdown files._ @@ -57,7 +57,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/benchmark --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-core/variantbenchmarking --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -82,7 +82,7 @@ Do not use `-c ` to specify parameters as this will result in errors. Cust The above pipeline run specified with a params file in yaml format: ```bash -nextflow run nf-core/benchmark -profile docker -params-file params.yaml +nextflow run nf-core/variantbenchmarking -profile docker -params-file params.yaml ``` with `params.yaml` containing: @@ -101,14 +101,14 @@ You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-c When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: ```bash -nextflow pull nf-core/benchmark +nextflow pull nf-core/variantbenchmarking ``` ### Reproducibility It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [nf-core/benchmark releases page](https://github.com/nf-core/benchmark/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. +First, go to the [nf-core/variantbenchmarking releases page](https://github.com/nf-core/variantbenchmarking/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index d13ecad..77e4235 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -1,5 +1,5 @@ // -// This file holds several functions specific to the main.nf workflow in the nf-core/benchmark pipeline +// This file holds several functions specific to the main.nf workflow in the nf-core/variantbenchmarking pipeline // import nextflow.Nextflow diff --git a/lib/WorkflowVariantbenchmarking.groovy b/lib/WorkflowVariantbenchmarking.groovy index 9f0a479..e563c58 100644 --- a/lib/WorkflowVariantbenchmarking.groovy +++ b/lib/WorkflowVariantbenchmarking.groovy @@ -1,5 +1,5 @@ // -// This file holds several functions specific to the workflow/svbench.nf in the nf-core/svbench pipeline +// This file holds several functions specific to the workflow/variantbenchmarking.nf in the nf-core/variantbenchmarking pipeline // import nextflow.Nextflow diff --git a/main.nf b/main.nf index 27d248d..2a99e0f 100644 --- a/main.nf +++ b/main.nf @@ -1,11 +1,11 @@ #!/usr/bin/env nextflow /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/svbench + nf-core/variantbenchmarking ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Github : https://github.com/nf-core/svbench - Website: https://nf-co.re/svbench - Slack : https://nfcore.slack.com/channels/svbench + Github : https://github.com/nf-core/variantbenchmarking + Website: https://nf-co.re/variantbenchmarking + Slack : https://nfcore.slack.com/channels/variantbenchmarking ---------------------------------------------------------------------------------------- */ @@ -53,13 +53,13 @@ WorkflowMain.initialise(workflow, params, log) ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { SVBENCH } from './workflows/svbench' +include { VARIANTBENCHMARKING } from './workflows/variantbenchmarking' // -// WORKFLOW: Run main nf-core/svbench analysis pipeline +// WORKFLOW: Run main nf-core/variantbenchmarking analysis pipeline // -workflow NFCORE_SVBENCH { - SVBENCH () +workflow NFCORE_VARIANTBENCHMARKING { + VARIANTBENCHMARKING () } /* @@ -73,7 +73,7 @@ workflow NFCORE_SVBENCH { // See: https://github.com/nf-core/rnaseq/issues/619 // workflow { - NFCORE_SVBENCH () + NFCORE_VARIANTBENCHMARKING () } /* diff --git a/modules.json b/modules.json index 881ab96..e608e79 100644 --- a/modules.json +++ b/modules.json @@ -1,6 +1,6 @@ { - "name": "nf-core/svbench", - "homePage": "https://github.com/nf-core/svbench", + "name": "nf-core/variantbenchmarking", + "homePage": "https://github.com/nf-core/variantbenchmarking", "repos": { "https://github.com/nf-core/modules.git": { "modules": { diff --git a/nextflow.config b/nextflow.config index 73dc5cf..1f4f8d7 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,6 +1,6 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - nf-core/svbench Nextflow config file + nf-core/variantbenchmarking Nextflow config file ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Default config options for all compute environments ---------------------------------------------------------------------------------------- @@ -23,8 +23,8 @@ params { // Benchmarking method method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist - // minsize effects both truvari and svbenchmark in different ways! svbechmark filters both base and comp calls - // although with truvari it is possible to filter only base, which makes more sense to me! + // minsize effects both truvari and variantbenchmarkingmark in different ways! svbechmark filters both base and comp calls + // although with truvari it is possible to filter only base, which makes more sense to me! // I will use this parameter to filter SVs before benchmarking! min_sv_size = 50 @@ -32,7 +32,7 @@ params { dup_to_ins = "" // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 // Manta params - bnd_to_inv = "" // manta reports INV as BND + bnd_to_inv = "" // manta reports INV as BND // Gridss params gridss_annotate = "" @@ -101,12 +101,12 @@ try { System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") } -// Load nf-core/svbench custom profiles from different institutions. +// Load nf-core/variantbenchmarking custom profiles from different institutions. // Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! // try { -// includeConfig "${params.custom_config_base}/pipeline/svbench.config" +// includeConfig "${params.custom_config_base}/pipeline/variantbenchmarking.config" // } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/svbench profiles: ${params.custom_config_base}/pipeline/svbench.config") +// System.err.println("WARNING: Could not load nf-core/config/variantbenchmarking profiles: ${params.custom_config_base}/pipeline/variantbenchmarking.config") // } profiles { debug { @@ -262,9 +262,9 @@ dag { } manifest { - name = 'nf-core/svbench' + name = 'nf-core/variantbenchmarking' author = """kuebra.narci@dkfz.de""" - homePage = 'https://github.com/nf-core/svbench' + homePage = 'https://github.com/nf-core/variantbenchmarking' description = """Master benchmarking pipeline for Structural Variant callers """ mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' diff --git a/nextflow_schema.json b/nextflow_schema.json index fbbe68f..9cb22d3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,7 +1,7 @@ { "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/svbench/master/nextflow_schema.json", - "title": "nf-core/svbench pipeline parameters", + "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", + "title": "nf-core/variantbenchmarking pipeline parameters", "description": "Master benchmarking pipeline for variant callers ", "type": "object", "definitions": { @@ -20,7 +20,7 @@ "schema": "assets/schema_input.json", "pattern": "^\\S+\\.(csv|tsv|yaml)$", "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/svbench/usage#samplesheet-input).", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/variantbenchmarking/usage#samplesheet-input).", "fa_icon": "fas fa-file-csv" }, "outdir": { diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 62c0b93..ea43fd9 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -82,7 +82,7 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft // Info required for completion email and summary def multiqc_report = [] -workflow SVBENCH { +workflow VARIANTBENCHMARKING { ch_versions = Channel.empty() @@ -94,7 +94,7 @@ workflow SVBENCH { ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) ch_input = INPUT_CHECK.out.ch_sample - + // // PREPARE_REGIONS: prepare stratifications and contigs // @@ -129,11 +129,11 @@ workflow SVBENCH { PREPARE_REGIONS.out.main_chroms, PREPARE_REGIONS.out.chr_list ) - ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) + ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) // // SUBWORKFLOW: GET STATISTICS OF FILES - // + // //REPORT_STATISTICS_TRUTH( // REPORT_STATISTICS_TEST( @@ -154,7 +154,7 @@ workflow SVBENCH { .map{it -> tuple(it[0],it[1], it[2], it[3], it[5], it[6], it[8])} .set{bench_ch} - // + // // SUBWORKFLOW: GERMLINE_BENCHMARK // //Benchmarking spesific to germline samples @@ -165,10 +165,10 @@ workflow SVBENCH { PREPARE_VCFS_TRUTH.out.vcf_ch ) ch_versions = ch_versions.mix(GERMLINE_BENCHMARK.out.versions) - + if (params.analysis.contains("somatic")){ - + // SOMATIC VARIANT BENCHMARKING SOMATIC_BENCHMARK( bench_ch, From a220d630b9499dd8605b818b1c78ade9c57c7736 Mon Sep 17 00:00:00 2001 From: Maxime U Garcia Date: Fri, 16 Feb 2024 17:25:42 +0100 Subject: [PATCH 003/292] Apply suggestions from code review --- README.md | 7 ++++++- workflows/variantbenchmarking.nf | 10 ++++++++-- 2 files changed, 14 insertions(+), 3 deletions(-) diff --git a/README.md b/README.md index 56b03b3..02fbed7 100644 --- a/README.md +++ b/README.md @@ -1,4 +1,9 @@ -# ![nf-core/variantbenchmarking](docs/images/nf-core-variantbenchmarking_logo_light.png#gh-light-mode-only) +

+ + + nf-core/variantbenchmarking + +

[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index ea43fd9..efe8d48 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -192,10 +192,10 @@ workflow VARIANTBENCHMARKING { // // MODULE: MultiQC // - workflow_summary = WorkflowBenchmark.paramsSummaryMultiqc(workflow, summary_params) + workflow_summary = WorkflowVariantbenchmarking.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) - methods_description = WorkflowBenchmark.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) + methods_description = WorkflowVariantbenchmarking.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) ch_methods_description = Channel.value(methods_description) ch_multiqc_files = Channel.empty() @@ -230,6 +230,12 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From bd7e23635bdaccc2e86783204a0a4178f1a7a4f0 Mon Sep 17 00:00:00 2001 From: Maxime U Garcia Date: Fri, 16 Feb 2024 17:25:58 +0100 Subject: [PATCH 004/292] Apply suggestions from code review --- assets/email_template.txt | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/email_template.txt b/assets/email_template.txt index 38114da..e9ad84c 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,7 +4,7 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/variantbenchmarking v${version} + nf-core/variantbenchmarking ${version} ---------------------------------------------------- Run Name: $runName From 7ff369e1c6c718e5a604b117fea583b36bb1ac61 Mon Sep 17 00:00:00 2001 From: Maxime U Garcia Date: Fri, 16 Feb 2024 17:26:40 +0100 Subject: [PATCH 005/292] Apply suggestions from code review --- README.md | 8 +++----- 1 file changed, 3 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index 02fbed7..b0b2898 100644 --- a/README.md +++ b/README.md @@ -75,11 +75,9 @@ nextflow run nf-core/variantbenchmarking \ --outdir ``` -:::warning -Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those -provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; -see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). -::: +> [!WARNING] +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; +> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). From dc274f54c6273f74f08330d44bdd345dfc48472f Mon Sep 17 00:00:00 2001 From: Maxime U Garcia Date: Fri, 16 Feb 2024 17:28:18 +0100 Subject: [PATCH 006/292] Apply suggestions from code review --- README.md | 7 ++----- 1 file changed, 2 insertions(+), 5 deletions(-) diff --git a/README.md b/README.md index b0b2898..a8a7b25 100644 --- a/README.md +++ b/README.md @@ -40,11 +40,8 @@ ## Usage -:::note -If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -with `-profile test` before running the workflow on actual data. -::: +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. Supported SV callers: Manta, SVaba, Dragen, Delly, Lumpy .. Available Truth samples: HG002, SEQC2 From 3a4c146ccce86a711f6f6a1b283a13d39628c0a8 Mon Sep 17 00:00:00 2001 From: maxulysse Date: Fri, 16 Feb 2024 17:30:28 +0100 Subject: [PATCH 007/292] file from TEMPLATE --- assets/email_template.html | 167 ++++++++++--------------------------- 1 file changed, 43 insertions(+), 124 deletions(-) diff --git a/assets/email_template.html b/assets/email_template.html index 3334523..ee343c4 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,134 +1,53 @@ - - - - + + + + - - nf-core/variantbenchmarking Pipeline Report - - -
- + + nf-core/variantbenchmarking Pipeline Report + + +
-

nf-core/variantbenchmarking v${version}

-

Run Name: $runName

+ - <% if (!success){ out << """ -
-

- nf-core/variantbenchmarking execution completed unsuccessfully! -

-

- The exit status of the task that caused the workflow execution to fail - was: $exitStatus. -

+

nf-core/variantbenchmarking ${version}

+

Run Name: $runName

+ +<% if (!success){ + out << """ +
+

nf-core/variantbenchmarking execution completed unsuccessfully!

+

The exit status of the task that caused the workflow execution to fail was: $exitStatus.

The full error message was:

- 
-${errorReport}
-
- """ } else { out << """ -
+ 
${errorReport}
+
+ """ +} else { + out << """ +
nf-core/variantbenchmarking execution completed successfully! -
- """ } %> +
+ """ +} +%> -

- The workflow was completed at $dateComplete (duration: - $duration) -

-

The command used to launch the workflow was as follows:

- 
-$commandLine
+

The workflow was completed at $dateComplete (duration: $duration)

+

The command used to launch the workflow was as follows:

+
$commandLine
-

Pipeline Configuration:

- - - <% out << summary.collect{ k,v -> " - - - - - " }.join("\n") %> - -
- $k - - 
$v
-
+

Pipeline Configuration:

+ + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
$k
$v
-

nf-core/variantbenchmarking

-

- https://github.com/nf-core/variantbenchmarking -

-
- +

nf-core/variantbenchmarking

+

https://github.com/nf-core/variantbenchmarking

+ +
+ + From 72cfc635bc7f4fd5d8078fceb1be8adeb0d87059 Mon Sep 17 00:00:00 2001 From: maxulysse Date: Fri, 16 Feb 2024 17:32:12 +0100 Subject: [PATCH 008/292] files from TEMPLATE --- lib/NfcoreSchema.groovy | 528 ---------------------------------------- lib/WorkflowMain.groovy | 16 +- 2 files changed, 15 insertions(+), 529 deletions(-) delete mode 100644 lib/NfcoreSchema.groovy diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy deleted file mode 100644 index 33cd4f6..0000000 --- a/lib/NfcoreSchema.groovy +++ /dev/null @@ -1,528 +0,0 @@ -// -// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. -// - -import org.everit.json.schema.Schema -import org.everit.json.schema.loader.SchemaLoader -import org.everit.json.schema.ValidationException -import org.json.JSONObject -import org.json.JSONTokener -import org.json.JSONArray -import groovy.json.JsonSlurper -import groovy.json.JsonBuilder - -class NfcoreSchema { - - // - // Resolve Schema path relative to main workflow directory - // - public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { - return "${workflow.projectDir}/${schema_filename}" - } - - // - // Function to loop over all parameters defined in schema and check - // whether the given parameters adhere to the specifications - // - /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { - def has_error = false - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Check for nextflow core params and unexpected params - def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text - def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') - def nf_params = [ - // Options for base `nextflow` command - 'bg', - 'c', - 'C', - 'config', - 'd', - 'D', - 'dockerize', - 'h', - 'log', - 'q', - 'quiet', - 'syslog', - 'v', - - // Options for `nextflow run` command - 'ansi', - 'ansi-log', - 'bg', - 'bucket-dir', - 'c', - 'cache', - 'config', - 'dsl2', - 'dump-channels', - 'dump-hashes', - 'E', - 'entry', - 'latest', - 'lib', - 'main-script', - 'N', - 'name', - 'offline', - 'params-file', - 'pi', - 'plugins', - 'poll-interval', - 'pool-size', - 'profile', - 'ps', - 'qs', - 'queue-size', - 'r', - 'resume', - 'revision', - 'stdin', - 'stub', - 'stub-run', - 'test', - 'w', - 'with-charliecloud', - 'with-conda', - 'with-dag', - 'with-docker', - 'with-mpi', - 'with-notification', - 'with-podman', - 'with-report', - 'with-singularity', - 'with-timeline', - 'with-tower', - 'with-trace', - 'with-weblog', - 'without-docker', - 'without-podman', - 'work-dir' - ] - def unexpectedParams = [] - - // Collect expected parameters from the schema - def expectedParams = [] - def enums = [:] - for (group in schemaParams) { - for (p in group.value['properties']) { - expectedParams.push(p.key) - if (group.value['properties'][p.key].containsKey('enum')) { - enums[p.key] = group.value['properties'][p.key]['enum'] - } - } - } - - for (specifiedParam in params.keySet()) { - // nextflow params - if (nf_params.contains(specifiedParam)) { - log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" - has_error = true - } - // unexpected params - def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' - def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } - def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { - // Temporarily remove camelCase/camel-case params #1035 - def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} - if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ - unexpectedParams.push(specifiedParam) - } - } - } - - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Validate parameters against the schema - InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() - JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) - - // Remove anything that's in params.schema_ignore_params - raw_schema = removeIgnoredParams(raw_schema, params) - - Schema schema = SchemaLoader.load(raw_schema) - - // Clean the parameters - def cleanedParams = cleanParameters(params) - - // Convert to JSONObject - def jsonParams = new JsonBuilder(cleanedParams) - JSONObject params_json = new JSONObject(jsonParams.toString()) - - // Validate - try { - schema.validate(params_json) - } catch (ValidationException e) { - println '' - log.error 'ERROR: Validation of pipeline parameters failed!' - JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, params_json, log, enums) - println '' - has_error = true - } - - // Check for unexpected parameters - if (unexpectedParams.size() > 0) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - println '' - def warn_msg = 'Found unexpected parameters:' - for (unexpectedParam in unexpectedParams) { - warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" - } - log.warn warn_msg - log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" - println '' - } - - if (has_error) { - System.exit(1) - } - } - - // - // Beautify parameters for --help - // - public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - Integer num_hidden = 0 - String output = '' - output += 'Typical pipeline command:\n\n' - output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - Integer max_chars = paramsMaxChars(params_map) + 1 - Integer desc_indent = max_chars + 14 - Integer dec_linewidth = 160 - desc_indent - for (group in params_map.keySet()) { - Integer num_params = 0 - String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (group_params.get(param).hidden && !params.show_hidden_params) { - num_hidden += 1 - continue; - } - def type = '[' + group_params.get(param).type + ']' - def description = group_params.get(param).description - def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : '' - def description_default = description + colors.dim + defaultValue + colors.reset - // Wrap long description texts - // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap - if (description_default.length() > dec_linewidth){ - List olines = [] - String oline = "" // " " * indent - description_default.split(" ").each() { wrd -> - if ((oline.size() + wrd.size()) <= dec_linewidth) { - oline += wrd + " " - } else { - olines += oline - oline = wrd + " " - } - } - olines += oline - description_default = olines.join("\n" + " " * desc_indent) - } - group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' - num_params += 1 - } - group_output += '\n' - if (num_params > 0){ - output += group_output - } - } - if (num_hidden > 0){ - output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset - } - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Groovy Map summarising parameters/workflow options used by the pipeline - // - public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { - // Get a selection of core Nextflow workflow options - def Map workflow_summary = [:] - if (workflow.revision) { - workflow_summary['revision'] = workflow.revision - } - workflow_summary['runName'] = workflow.runName - if (workflow.containerEngine) { - workflow_summary['containerEngine'] = workflow.containerEngine - } - if (workflow.container) { - workflow_summary['container'] = workflow.container - } - workflow_summary['launchDir'] = workflow.launchDir - workflow_summary['workDir'] = workflow.workDir - workflow_summary['projectDir'] = workflow.projectDir - workflow_summary['userName'] = workflow.userName - workflow_summary['profile'] = workflow.profile - workflow_summary['configFiles'] = workflow.configFiles.join(', ') - - // Get pipeline parameters defined in JSON Schema - def Map params_summary = [:] - def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - for (group in params_map.keySet()) { - def sub_params = new LinkedHashMap() - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (params.containsKey(param)) { - def params_value = params.get(param) - def schema_value = group_params.get(param).default - def param_type = group_params.get(param).type - if (schema_value != null) { - if (param_type == 'string') { - if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { - def sub_string = schema_value.replace('\$projectDir', '') - sub_string = sub_string.replace('\${projectDir}', '') - if (params_value.contains(sub_string)) { - schema_value = params_value - } - } - if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { - def sub_string = schema_value.replace('\$params.outdir', '') - sub_string = sub_string.replace('\${params.outdir}', '') - if ("${params.outdir}${sub_string}" == params_value) { - schema_value = params_value - } - } - } - } - - // We have a default in the schema, and this isn't it - if (schema_value != null && params_value != schema_value) { - sub_params.put(param, params_value) - } - // No default in the schema, and this isn't empty - else if (schema_value == null && params_value != "" && params_value != null && params_value != false) { - sub_params.put(param, params_value) - } - } - } - params_summary.put(group, sub_params) - } - return [ 'Core Nextflow options' : workflow_summary ] << params_summary - } - - // - // Beautify parameters for summary and return as string - // - public static String paramsSummaryLog(workflow, params) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - String output = '' - def params_map = paramsSummaryMap(workflow, params) - def max_chars = paramsMaxChars(params_map) - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - if (group_params) { - output += colors.bold + group + colors.reset + '\n' - for (param in group_params.keySet()) { - output += " " + colors.blue + param.padRight(max_chars) + ": " + colors.green + group_params.get(param) + colors.reset + '\n' - } - output += '\n' - } - } - output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Loop over nested exceptions and print the causingException - // - private static void printExceptions(ex_json, params_json, log, enums, limit=5) { - def causingExceptions = ex_json['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (ex_json['pointerToViolation'] == '#') { - log.error "* ${ex_json['message']}" - } - // Error with specific param - else { - def param = ex_json['pointerToViolation'] - ~/^#\// - def param_val = params_json[param].toString() - if (enums.containsKey(param)) { - def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices" - if (enums[param].size() > limit) { - log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )" - } else { - log.error "${error_msg}: ${enums[param].join(', ')})" - } - } else { - log.error "* --${param}: ${ex_json['message']} (${param_val})" - } - } - } - for (ex in causingExceptions) { - printExceptions(ex, params_json, log, enums) - } - } - - // - // Remove an element from a JSONArray - // - private static JSONArray removeElement(json_array, element) { - def list = [] - int len = json_array.length() - for (int i=0;i - if(raw_schema.keySet().contains('definitions')){ - raw_schema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { - raw_schema.get("properties").remove(ignore_param) - } - if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { - def cleaned_required = removeElement(raw_schema.required, ignore_param) - raw_schema.put("required", cleaned_required) - } - } - return raw_schema - } - - // - // Clean and check parameters relative to Nextflow native classes - // - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - // - // This function tries to read a JSON params file - // - private static LinkedHashMap paramsLoad(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = paramsRead(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - // - // Method to actually read in JSON file using Groovy. - // Group (as Key), values are all parameters - // - Parameter1 as Key, Description as Value - // - Parameter2 as Key, Description as Value - // .... - // Group - // - - private static LinkedHashMap paramsRead(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - // - // Get maximum number of characters across all parameter names - // - private static Integer paramsMaxChars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 77e4235..025eed8 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -24,7 +24,7 @@ class WorkflowMain { // // Validate parameters and print summary to screen // - public static void initialise(workflow, params, log) { + public static void initialise(workflow, params, log, args) { // Print workflow version and exit on --version if (params.version) { @@ -35,6 +35,8 @@ class WorkflowMain { // Check that a -profile or Nextflow config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) // Check that conda channels are set-up correctly if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { @@ -60,4 +62,16 @@ class WorkflowMain { } return null } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } From 652b4fdc09a49e1074a1346276b0e45938bf142c Mon Sep 17 00:00:00 2001 From: Maxime U Garcia Date: Fri, 16 Feb 2024 17:33:12 +0100 Subject: [PATCH 009/292] Apply suggestions from code review --- lib/WorkflowVariantbenchmarking.groovy | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/lib/WorkflowVariantbenchmarking.groovy b/lib/WorkflowVariantbenchmarking.groovy index e563c58..ae9238e 100644 --- a/lib/WorkflowVariantbenchmarking.groovy +++ b/lib/WorkflowVariantbenchmarking.groovy @@ -5,7 +5,7 @@ import nextflow.Nextflow import groovy.text.SimpleTemplateEngine -class WorkflowBenchmark { +class WorkflowVariantbenchmarking { // // Check and validate parameters From ae05f888fd68e008d3c8262dbf2622ad9c82d463 Mon Sep 17 00:00:00 2001 From: Maxime U Garcia Date: Fri, 16 Feb 2024 17:33:36 +0100 Subject: [PATCH 010/292] Apply suggestions from code review --- main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 2a99e0f..e7cf458 100644 --- a/main.nf +++ b/main.nf @@ -45,7 +45,7 @@ if (params.validate_params) { validateParameters() } -WorkflowMain.initialise(workflow, params, log) +WorkflowMain.initialise(workflow, params, log, args) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From 181c819f85db9de9421d227b10a59211f1dcf219 Mon Sep 17 00:00:00 2001 From: Maxime U Garcia Date: Fri, 16 Feb 2024 17:38:15 +0100 Subject: [PATCH 011/292] Apply suggestions from code review --- workflows/variantbenchmarking.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index efe8d48..6ec48fe 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -13,7 +13,7 @@ def summary_params = paramsSummaryMap(workflow) // Print parameter summary log to screen log.info logo + paramsSummaryLog(workflow) + citation -WorkflowBenchmark.initialise(params, log) +WorkflowVariantbenchmarking.initialise(params, log) // check mandatory parameters ref = Channel.fromPath([params.fasta,params.fai], checkIfExists: true).collect() @@ -236,6 +236,7 @@ workflow.onError { println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") } } + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From 3c1c6507c010a5cbd989df298c0bbca79ddedfd0 Mon Sep 17 00:00:00 2001 From: maxulysse Date: Fri, 16 Feb 2024 17:36:40 +0100 Subject: [PATCH 012/292] update file --- modules/local/samplesheet_check.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 6424a24..87d88f4 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK { conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'quay.io/biocontainers/python:3.8.3' }" + 'biocontainers/python:3.8.3' }" input: path samplesheet @@ -17,7 +17,7 @@ process SAMPLESHEET_CHECK { when: task.ext.when == null || task.ext.when - script: // This script is bundled with the pipeline, in nf-core/benchmark/bin/ + script: // This script is bundled with the pipeline, in nf-core/variantbenchmarking/bin/ """ check_samplesheet.py \\ $samplesheet \\ From 6a6ce9c6f5032f274d7d1612f728ac49e534c99f Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 19 Feb 2024 15:36:56 +0100 Subject: [PATCH 013/292] meta2 is removed --- conf/modules.config | 73 ++++---- conf/test_hg38.config | 3 +- modules/local/bcftools_view.nf | 4 +- modules/local/bgzip_tabix.nf | 6 +- modules/local/gridss_annotation.nf | 56 +++--- modules/local/samplesheet_check.nf | 2 +- modules/local/vcfdist.nf | 6 +- modules/local/wittyer.nf | 8 +- modules/nf-core/bcftools/norm/main.nf | 6 +- modules/nf-core/bcftools/reheader/main.nf | 6 +- modules/nf-core/bcftools/stats/main.nf | 8 +- modules/nf-core/bcftools/view/main.nf | 6 +- .../nf-core/manta/convertinversion/main.nf | 4 +- modules/nf-core/survivor/filter/main.nf | 8 +- modules/nf-core/survivor/stats/main.nf | 6 +- .../nf-core/svanalyzer/svbenchmark/main.nf | 14 +- modules/nf-core/svync/main.nf | 4 +- modules/nf-core/tabix/bgzip/main.nf | 6 +- modules/nf-core/tabix/bgziptabix/main.nf | 6 +- modules/nf-core/tabix/tabix/main.nf | 6 +- modules/nf-core/truvari/bench/main.nf | 4 +- .../local/germline_benchmark_backup.nf | 162 ------------------ subworkflows/local/input_check.nf | 30 ++-- subworkflows/local/prepare_regions.nf | 44 ----- subworkflows/local/prepare_vcfs_test.nf | 16 +- subworkflows/local/prepare_vcfs_truth.nf | 10 +- subworkflows/local/vcf_conversion.nf | 80 ++++----- workflows/variantbenchmarking.nf | 25 +-- 28 files changed, 170 insertions(+), 439 deletions(-) delete mode 100644 subworkflows/local/germline_benchmark_backup.nf delete mode 100644 subworkflows/local/prepare_regions.nf diff --git a/conf/modules.config b/conf/modules.config index 6e27229..3711745 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -43,8 +43,9 @@ process { ] } withName: "BCFTOOLS_NORM_1" { + ext.prefix = {"${params.sample}"} ext.args = {"--output-type z -N -m-any -c w" } - ext.prefix = {"${meta.id}.${meta2.caller}_norm"} + ext.prefix = {"${meta.id}_norm"} publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", @@ -52,24 +53,18 @@ process { ] } withName: "BCFTOOLS_NORM_2" { + ext.prefix = {"${params.sample}"} ext.args = {"--output-type z --rm-du exact -c w" } - ext.prefix = {"${meta.id}.${meta2.caller}_dedup"} + ext.prefix = {"${meta.id}_dedup"} publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", mode: params.publish_dir_mode ] } - withName: AWK_SORT { - ext.prefix = {"${meta.id}.${meta2.caller}_sort"} - publishDir = [ - path: { "${params.outdir}/test" }, - enabled: false - ] - } withName: BCFTOOLS_RENAME_CHR { ext.args = {"--output-type z" } - ext.prefix = {"${meta.id}.${meta2.caller}_rename_chr"} + ext.prefix = {"${meta.id}_rename_chr"} publishDir = [ path: { "${params.outdir}/test" }, enabled: false @@ -92,24 +87,17 @@ process { ] } withName: BCFTOOLS_VIEW { + ext.prefix = {"${params.sample}"} ext.args2 = {"--output-type z" } - ext.prefix = {"${meta.id}.${meta2.caller}_filter"} + ext.prefix = {"${meta.id}_filter"} publishDir = [ - path: {"${params.outdir}/${meta.id}/"}, + path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", mode: params.publish_dir_mode ] } - withName: BCFTOOLS_ISEC { - ext.args = {"--output-type v" } - ext.prefix = {"${meta.id}.${meta2.caller}"} - publishDir = [ - path: {"${params.outdir}/${meta.id}/svanalyzer_bench/${meta2.caller}/isec"}, - mode: params.publish_dir_mode - ] - } withName: SURVIVOR_STATS { - ext.prefix = {"${meta.id}.${meta2.caller}"} + ext.prefix = {"${params.sample}"} publishDir = [ path: {"${params.outdir}/${meta.id}/stats/survivor/"}, pattern: "*{.stats}", @@ -117,7 +105,7 @@ process { ] } withName: SURVIVOR_FILTER { - ext.prefix = {"${meta.id}.${meta2.caller}.filter"} + ext.prefix = {"${params.sample}.filter"} publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf}", @@ -125,7 +113,7 @@ process { ] } withName: BCFTOOLS_STATS { - ext.prefix = {"${meta.id}.${meta2.caller}"} + ext.prefix = {"${params.sample}.filter"} publishDir = [ path: {"${params.outdir}/${meta.id}/stats/bcftools/"}, pattern: "*{stats.txt}", @@ -133,75 +121,76 @@ process { ] } withName: "TRUVARI_PHAB" { - ext.prefix = {"${meta.id}.${meta2.caller}.harm"} + ext.prefix = {"${params.sample}"} + ext.prefix = {"${meta.id}.harm"} publishDir = [ - path: {"${params.outdir}/${meta.id}/truvari_phab/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/truvari_phab/"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", mode: params.publish_dir_mode ] } withName: "TRUVARI_BENCH" { + ext.prefix = {"${params.sample}"} ext.args = {"--pctsize 0.5 --pctovl 0.5 --refdist 1000"} - ext.prefix = {"${meta.id}.${meta2.caller}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/truvari_bench/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/truvari_bench"}, pattern: "*{.vcf.gz,vcf.gz.tbi,json}", mode: params.publish_dir_mode ] } withName: SVANALYZER_SVBENCHMARK { + ext.prefix = {"${params.sample}"} ext.args = {"-normshift 0.3 –normdist 0.3 –normsizediff 0.3"} - ext.prefix = {"${meta.id}.${meta2.caller}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/svanalyzer_bench/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/svanalyzer_bench"}, pattern: "*{.vcf,distances,log,report}", mode: params.publish_dir_mode ] } withName: WITTYER { + ext.prefix = {"${params.sample}"} ext.args = {"--evaluationMode=CrossTypeAndSimpleCounting --percentDistance=0.3 --bpDistance=1000"} - ext.prefix = {"${meta.id}.${meta2.caller}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/wittyer_bench/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/wittyer_bench"}, pattern: "*{json,.vcf.gz.tbi,vcf.gz}", mode: params.publish_dir_mode ] } withName: VCFDIST { + ext.prefix = {"${params.sample}"} ext.args = {"-v 0"} - ext.prefix = {"${meta.id}.${meta2.caller}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/vcfdist_bench/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/vcfdist_bench"}, pattern: "*{.vcf,tsv}", mode: params.publish_dir_mode ] } withName: BAMSURGEON_EVALUATOR { - ext.prefix = {"${meta.id}.${meta2.caller}"} + ext.prefix = {"${params.sample}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/bamsurgeon_evalator/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/bamsurgeon_evalator"}, pattern: "*{.vcf}", mode: params.publish_dir_mode ] } withName: HAPPY_SOMPY { - ext.prefix = {"${meta.id}.${meta2.caller}"} + ext.prefix = {"${params.sample}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/sompy_bench/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/sompy_bench"}, pattern: "*{.vcf.gz,vcf.gz.tbi,json,csv}", mode: params.publish_dir_mode ] } withName: MANTA_CONVERTINVERSION { - ext.prefix = {"${meta.id}.${meta2.caller}"} + ext.prefix = {"${params.sample}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess/${meta2.caller}"}, + path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", mode: params.publish_dir_mode ] } withName: SVYNC { - ext.prefix = {"${meta.id}.${meta2.caller}.stnd"} + ext.prefix = {"${params.sample}.stnd"} publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", @@ -212,8 +201,8 @@ process { // // Don't publish results for these processes // -process { - withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX' { +process { + withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX|TABIX_BGZIP' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false diff --git a/conf/test_hg38.config b/conf/test_hg38.config index 3336ae6..afb8c82 100644 --- a/conf/test_hg38.config +++ b/conf/test_hg38.config @@ -30,10 +30,11 @@ params { // Processes analysis = 'germline' //somatic - method = 'truvari,svanalyzer,wittyer,vcfdist' // --not working for now : wittyer, vcfdist + method = 'truvari,svanalyzer,wittyer' // --not working for now : vcfdist //harmonize = true // ATTENTION : takes long time because of multi-alignment process. similarity = 0 // determines the sequence similarity level in benchmarking. preprocess = "normalization, deduplication" + min_sv_size = 50 // Truvari params //dup_to_ins = true // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 diff --git a/modules/local/bcftools_view.nf b/modules/local/bcftools_view.nf index eab11f7..71e1fa2 100644 --- a/modules/local/bcftools_view.nf +++ b/modules/local/bcftools_view.nf @@ -8,11 +8,11 @@ process BCFTOOLS_VIEW { 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta),path(vcf), path(index) output: - tuple val(meta),val(meta2), path("*.vcf") , emit: vcf + tuple val(meta), path("*.vcf") , emit: vcf path "versions.yml" , emit: versions when: diff --git a/modules/local/bgzip_tabix.nf b/modules/local/bgzip_tabix.nf index 887da5f..f7f32fb 100644 --- a/modules/local/bgzip_tabix.nf +++ b/modules/local/bgzip_tabix.nf @@ -8,11 +8,11 @@ process BGZIP_TABIX { 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" input: - tuple val(meta),val(meta2), path(vcf) + tuple val(meta),path(vcf) output: - tuple val(meta),val(meta2), path("*.vcf.gz"), path("*.tbi"), emit: gz_tbi - path "versions.yml" , emit: versions + tuple val(meta),path("*.vcf.gz"), path("*.tbi"), emit: gz_tbi + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/local/gridss_annotation.nf b/modules/local/gridss_annotation.nf index 317f5a6..523d4bb 100644 --- a/modules/local/gridss_annotation.nf +++ b/modules/local/gridss_annotation.nf @@ -1,6 +1,6 @@ // UNTESTED process GRIDSS_ANNOTATION { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_medium' conda "${moduleDir}/environment.yml" @@ -9,11 +9,11 @@ process GRIDSS_ANNOTATION { 'quay.io/biocontainers/gridss:2.13.2--h270b39a_0' }" input: - tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta),path(vcf), path(index) tuple path(fasta), path(fasta_fai) output: - tuple val(meta),val(meta2), path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf + tuple val(meta),path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf path "versions.yml" , emit: versions when: @@ -22,39 +22,25 @@ process GRIDSS_ANNOTATION { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def genome = params.genome.contains("38") ? "hg38": "hg19" + def genome = params.genome.contains("38") ? "hg38": "hg19" def VERSION = '2.13.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. - if (meta2.caller == "gridss"){ - """ - bgzip -d $vcf -c > unzziped.vcf - simple_event-annotator.R \\ - unzziped.vcf \\ - ${prefix}.vcf \\ - ${genome} - - bgzip --threads ${task.cpus} -c ${prefix}.vcf > ${prefix}.anno.vcf.gz - tabix -p vcf ${prefix}.anno.vcf.gz - - rm unzziped.vcf - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - gridss: ${VERSION} - END_VERSIONS - """ - } - else{ - """ - cp $vcf ${prefix}.vcf.gz - cp $index ${prefix}.vcf.gz.tbi - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - gridss: ${VERSION} - END_VERSIONS - """ - - } + """ + bgzip -d $vcf -c > unzziped.vcf + simple_event-annotator.R \\ + unzziped.vcf \\ + ${prefix}.vcf \\ + ${genome} + + bgzip --threads ${task.cpus} -c ${prefix}.vcf > ${prefix}.anno.vcf.gz + tabix -p vcf ${prefix}.anno.vcf.gz + + rm unzziped.vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gridss: ${VERSION} + END_VERSIONS + """ } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 87d88f4..daead49 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK { conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" + 'quay.io/biocontainers/python:3.8.3' }" input: path samplesheet diff --git a/modules/local/vcfdist.nf b/modules/local/vcfdist.nf index 0ae9939..a2771c7 100644 --- a/modules/local/vcfdist.nf +++ b/modules/local/vcfdist.nf @@ -1,5 +1,5 @@ process VCFDIST { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_single' conda "" @@ -8,7 +8,7 @@ process VCFDIST { 'timd1/vcfdist:v2.3.2' }" input: - tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple val(meta),path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) tuple path(fasta), path(fai) output: @@ -37,5 +37,5 @@ process VCFDIST { vcfdist: \$(echo \$(vcfdist --version 2>&1) | sed 's/^.*vcfdist v//') END_VERSIONS """ - + } diff --git a/modules/local/wittyer.nf b/modules/local/wittyer.nf index 20db34a..57973e7 100644 --- a/modules/local/wittyer.nf +++ b/modules/local/wittyer.nf @@ -1,5 +1,5 @@ process WITTYER { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_single' conda "" @@ -8,14 +8,14 @@ process WITTYER { 'kubran/wittyer:0.3.3.0' }" input: - tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple val(meta),path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) path(config) output: tuple val(meta), path("*ConfigFileUsed.json") , emit: config tuple val(meta), path("*.Stats.json") , emit: report tuple val(meta), path("*eval.vcf.gz") , emit: bench_vcf - tuple val(meta), path("*eval.vcf.gz.tbi") , emit: bench_vcf_gzi + tuple val(meta), path("*eval.vcf.gz.tbi") , emit: bench_vcf_gzi path "versions.yml" , emit: versions when: @@ -49,5 +49,5 @@ process WITTYER { wittyer: 0.3.3.0 END_VERSIONS """ - + } diff --git a/modules/nf-core/bcftools/norm/main.nf b/modules/nf-core/bcftools/norm/main.nf index 379acdb..5127bba 100644 --- a/modules/nf-core/bcftools/norm/main.nf +++ b/modules/nf-core/bcftools/norm/main.nf @@ -8,13 +8,13 @@ process BCFTOOLS_NORM { 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" input: - tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta), path(vcf), path(index) tuple path(fasta), path(fai) tuple val(meta3), path(regions) output: - tuple val(meta),val(meta2), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf - path "versions.yml" , emit: versions + tuple val(meta),path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/bcftools/reheader/main.nf b/modules/nf-core/bcftools/reheader/main.nf index a60248c..5f01eff 100644 --- a/modules/nf-core/bcftools/reheader/main.nf +++ b/modules/nf-core/bcftools/reheader/main.nf @@ -1,5 +1,5 @@ process BCFTOOLS_REHEADER { - tag "$meta.id $meta2.caller" + tag "$meta.id " label 'process_low' conda "${moduleDir}/environment.yml" @@ -8,11 +8,11 @@ process BCFTOOLS_REHEADER { 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta),path(vcf), path(index) tuple path(fasta), path(fai) output: - tuple val(meta),val(meta2), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + tuple val(meta),path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/bcftools/stats/main.nf b/modules/nf-core/bcftools/stats/main.nf index 8a38397..2e0084b 100644 --- a/modules/nf-core/bcftools/stats/main.nf +++ b/modules/nf-core/bcftools/stats/main.nf @@ -1,5 +1,5 @@ process BCFTOOLS_STATS { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_single' conda "${moduleDir}/environment.yml" @@ -8,7 +8,7 @@ process BCFTOOLS_STATS { 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta), val(meta2), path(vcf), path(index) + tuple val(meta), path(vcf), path(index) tuple val(meta3), path(regions) tuple val(meta4), path(targets) tuple val(meta5), path(samples) @@ -16,8 +16,8 @@ process BCFTOOLS_STATS { tuple val(meta7), path(fasta) output: - tuple val(meta),val(meta2), path("*stats.txt"), emit: stats - path "versions.yml" , emit: versions + tuple val(meta), path("*stats.txt"), emit: stats + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf index 63b44c8..b62f56a 100644 --- a/modules/nf-core/bcftools/view/main.nf +++ b/modules/nf-core/bcftools/view/main.nf @@ -8,14 +8,14 @@ process BCFTOOLS_VIEW { 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta),path(vcf), path(index) tuple val(meta3), path(regions) path(targets) path(samples) output: - tuple val(meta),val(meta2), path("*.gz") , emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.gz") , emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/manta/convertinversion/main.nf b/modules/nf-core/manta/convertinversion/main.nf index 9010c3c..f771b74 100644 --- a/modules/nf-core/manta/convertinversion/main.nf +++ b/modules/nf-core/manta/convertinversion/main.nf @@ -9,11 +9,11 @@ process MANTA_CONVERTINVERSION { 'quay.io/biocontainers/mulled-v2-40295ae41112676b05b649e513fe7000675e9b84:a0332aa38645fbb8969567731ce68cfb7f830ec4-0' }" input: - tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta), path(vcf), path(index) tuple path(fasta), path(fai) output: - tuple val(meta),val(meta2), path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf_tabi + tuple val(meta), path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf_tabi path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/survivor/filter/main.nf b/modules/nf-core/survivor/filter/main.nf index d9c2557..a85bf62 100644 --- a/modules/nf-core/survivor/filter/main.nf +++ b/modules/nf-core/survivor/filter/main.nf @@ -1,5 +1,5 @@ process SURVIVOR_FILTER { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_single' conda "${moduleDir}/environment.yml" @@ -8,15 +8,15 @@ process SURVIVOR_FILTER { 'quay.io/biocontainers/survivor:1.0.7--h9a82719_1' }" input: - tuple val(meta),val(meta2), path(vcf_file), path(bed) // VCF file to filter and BED file with regions to ignore (NA to disable) + tuple val(meta),path(vcf_file), path(bed) // VCF file to filter and BED file with regions to ignore (NA to disable) val(minsv) // Min SV size (-1 to disable) val(maxsv) // Max SV size (-1 to disable) val(minallelefreq) // Min allele frequency (0-1) val(minnumreads) // Min number of reads support: RE flag (-1 to disable) output: - tuple val(meta),val(meta2), path("*.vcf"), emit: vcf - path "versions.yml" , emit: versions + tuple val(meta),path("*.vcf"), emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/survivor/stats/main.nf b/modules/nf-core/survivor/stats/main.nf index 528e94c..1b41304 100644 --- a/modules/nf-core/survivor/stats/main.nf +++ b/modules/nf-core/survivor/stats/main.nf @@ -1,5 +1,5 @@ process SURVIVOR_STATS { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_single' conda "${moduleDir}/environment.yml" @@ -8,13 +8,13 @@ process SURVIVOR_STATS { 'quay.io/biocontainers/survivor:1.0.7--h9a82719_1' }" input: - tuple val(meta),val(meta2), path(vcf), path(index) + tuple val(meta), path(vcf), path(index) val(minsv) // Min SV size (-1 to disable) val(maxsv) // Max SV size (-1 to disable) val(minnumreads) // Min number of reads support: RE flag (-1 to disable) output: - tuple val(meta),val(meta2), path("*.stats"), emit: stats + tuple val(meta),path("*.stats"), emit: stats path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/svanalyzer/svbenchmark/main.nf b/modules/nf-core/svanalyzer/svbenchmark/main.nf index afbcd9b..949e2cc 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/main.nf +++ b/modules/nf-core/svanalyzer/svbenchmark/main.nf @@ -1,6 +1,6 @@ process SVANALYZER_SVBENCHMARK { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_medium' conda "bioconda::svanalyzer=0.35" @@ -9,15 +9,15 @@ process SVANALYZER_SVBENCHMARK { 'quay.io/biocontainers/svanalyzer:0.35--pl526_0' }" input: - tuple val(meta),val(meta2), path(test), path(test_index), path(truth), path(truth_index), path(bed) + tuple val(meta),path(test), path(test_index), path(truth), path(truth_index), path(bed) tuple path(fasta), path(fai) output: - tuple val(meta),val(meta2), path("*.falsenegatives.vcf"), emit: fns - tuple val(meta),val(meta2), path("*.falsepositives.vcf"), emit: fps - tuple val(meta),val(meta2), path("*.distances") , emit: distances - tuple val(meta),val(meta2), path("*.log") , emit: log - tuple val(meta),val(meta2), path("*.report") , emit: report + tuple val(meta),path("*.falsenegatives.vcf"), emit: fns + tuple val(meta),path("*.falsepositives.vcf"), emit: fps + tuple val(meta),path("*.distances") , emit: distances + tuple val(meta),path("*.log") , emit: log + tuple val(meta), path("*.report") , emit: report path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/svync/main.nf b/modules/nf-core/svync/main.nf index d28e526..66c2f1a 100644 --- a/modules/nf-core/svync/main.nf +++ b/modules/nf-core/svync/main.nf @@ -8,10 +8,10 @@ process SVYNC { 'quay.io/biocontainers/svync:0.1.2--h9ee0642_0' }" input: - tuple val(meta),val(meta2), path(vcf), path(tbi), path(config) + tuple val(meta),path(vcf), path(tbi), path(config) output: - tuple val(meta),val(meta2), path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: vcf + tuple val(meta),path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: vcf path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf index 8e22f38..346c1ba 100644 --- a/modules/nf-core/tabix/bgzip/main.nf +++ b/modules/nf-core/tabix/bgzip/main.nf @@ -8,11 +8,11 @@ process TABIX_BGZIP { 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" input: - tuple val(meta),val(meta2), path(input) + tuple val(meta),path(input) output: - tuple val(meta),val(meta2), path("${output}") , emit: output - tuple val(meta),val(meta2), path("${output}.gzi"), emit: gzi, optional: true + tuple val(meta),path("${output}") , emit: output + tuple val(meta),path("${output}.gzi"), emit: gzi, optional: true path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf index 3a099b8..07f442d 100644 --- a/modules/nf-core/tabix/bgziptabix/main.nf +++ b/modules/nf-core/tabix/bgziptabix/main.nf @@ -8,11 +8,11 @@ process TABIX_BGZIPTABIX { 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" input: - tuple val(meta),val(meta2), path(input) + tuple val(meta),path(input) output: - tuple val(meta),val(meta2), path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi - tuple val(meta),val(meta2), path("*.gz"), path("*.csi"), optional: true, emit: gz_csi + tuple val(meta),path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi + tuple val(meta),path("*.gz"), path("*.csi"), optional: true, emit: gz_csi path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf index 3c6dea7..58dbd83 100644 --- a/modules/nf-core/tabix/tabix/main.nf +++ b/modules/nf-core/tabix/tabix/main.nf @@ -8,11 +8,11 @@ process TABIX_TABIX { 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" input: - tuple val(meta),val(meta2), path(tab) + tuple val(meta),path(tab) output: - tuple val(meta),val(meta2), path("*.tbi"), optional:true, emit: tbi - tuple val(meta),val(meta2), path("*.csi"), optional:true, emit: csi + tuple val(meta),path("*.tbi"), optional:true, emit: tbi + tuple val(meta),path("*.csi"), optional:true, emit: csi path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/truvari/bench/main.nf b/modules/nf-core/truvari/bench/main.nf index b0831d0..4e1a104 100644 --- a/modules/nf-core/truvari/bench/main.nf +++ b/modules/nf-core/truvari/bench/main.nf @@ -1,5 +1,5 @@ process TRUVARI_BENCH { - tag "$meta.id $meta2.caller" + tag "$meta.id" label 'process_single' conda "${moduleDir}/environment.yml" @@ -8,7 +8,7 @@ process TRUVARI_BENCH { 'quay.io/biocontainers/truvari:4.1.0--pyhdfd78af_0' }" input: - tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple val(meta),path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) tuple path(fasta), path(fai) output: diff --git a/subworkflows/local/germline_benchmark_backup.nf b/subworkflows/local/germline_benchmark_backup.nf deleted file mode 100644 index 59607f8..0000000 --- a/subworkflows/local/germline_benchmark_backup.nf +++ /dev/null @@ -1,162 +0,0 @@ -// -// GERMLINE: SUBWORKFLOW FOR GERMLINE VARIANTS -// - -params.options = [:] - -include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' addParams( options: params.options ) -include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' addParams( options: params.options ) -include { WITTYER } from '../../modules/local/wittyer' addParams( options: params.options ) -include { VCFDIST } from '../../modules/local/vcfdist' addParams( options: params.options ) -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_QUERY } from '../../modules/local/bcftools_view' addParams( options: params.options ) -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_TRUTH } from '../../modules/local/bcftools_view' addParams( options: params.options ) -include { ADDHEAD as ADDHEAD_TRUTH } from '../../modules/local/addhead' addParams( options: params.options ) -include { ADDHEAD as ADDHEAD_QUERY } from '../../modules/local/addhead' addParams( options: params.options ) -include { BCFTOOLS_ISEC as BCFTOOLS_ISEC_TRUTH } from '../../modules/nf-core/bcftools/isec' addParams( options: params.options ) -include { BCFTOOLS_ISEC as BCFTOOLS_ISEC_QUERY } from '../../modules/nf-core/bcftools/isec' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) - -workflow GERMLINE_BENCHMARK { - take: - input_ch // channel: [val(meta),val(meta2), test_vcf, test_index , truth_vcf, truth_index] - bed // channel: bed - ref // reference channel [ref.fa, ref.fa.fai] - truth_vcf // channel: [val(meta),val(meta2),truth_vcf, truth_index] - - main: - - versions=Channel.empty() - - // SV benchmarking - - if (params.method.contains('truvari')){ - // - // MODULE: TRUVARI_BENCH - // - TRUVARI_BENCH( - input_ch, - bed, - ref - ) - versions = versions.mix(TRUVARI_BENCH.out.versions) - } - - if (params.method.contains('svanalyzer')){ - // - // MODULE: SVANALYZER_SVBENCHMARK - // - // slower than truvari - SVANALYZER_SVBENCHMARK( - input_ch, - ref, - bed - ) - versions = versions.mix(SVANALYZER_SVBENCHMARK.out.versions) - - // get the original headers from the vcfs - // - // MODULE: BCFTOOLS_VIEW - // - BCFTOOLS_VIEW_QUERY( - input_ch.map{it -> tuple(it[0],it[1], it[2], it[3])} - ) - query_header = BCFTOOLS_VIEW_QUERY.out.header - - BCFTOOLS_VIEW_TRUTH( - truth_vcf - ) - truth_header = BCFTOOLS_VIEW_TRUTH.out.header - versions = versions.mix(BCFTOOLS_VIEW_QUERY.out.versions) - // - // MODULE: BCFTOOLS_REHEADER - // - SVANALYZER_SVBENCHMARK.out.fns.combine(truth_header, by:0) - .map{it -> tuple(it[0],it[1], it[2], it[4])} - .set{fns_header} - fns_header.view() - ADDHEAD_TRUTH( - fns_header - ) - - TABIX_TABIX_1( - ADDHEAD_TRUTH.out.vcf - ) - - ADDHEAD_TRUTH.out.vcf.join(TABIX_TABIX_1.out.tbi, by:1) - .map{it -> tuple( it[1], it[0], it[2], it[4])} - .set{fns_vcf} - - ////// - SVANALYZER_SVBENCHMARK.out.fps.combine(query_header, by:0) - .map{it -> tuple(it[0],it[1], it[2], it[4])} - .set{fps_header} - - ADDHEAD_QUERY( - fps_header - ) - versions = versions.mix(ADDHEAD_QUERY.out.versions) - - TABIX_TABIX_2( - ADDHEAD_QUERY.out.vcf - ) - - ADDHEAD_QUERY.out.vcf.join(TABIX_TABIX_2.out.tbi, by:1) - .map{it -> tuple( it[1], it[0], it[2], it[4])} - .set{fps_ch} - // - // MODULE: BCFTOOLS_ISEC - // - - // Find TP_comp (query) - input_ch.map{it -> tuple(it[0],it[1], it[2], it[3])} - .combine(fps_ch, by:0) - .map{it -> tuple(it[0],it[1], it[2], it[5], it[3], it[6])} - .set{query_ch} - - BCFTOOLS_ISEC_QUERY( - query_ch - ) - - // Find TP_comp (query) - truth_vcf.combine(fns_vcf, by:0) - .map{it -> tuple(it[0],it[1], it[2], it[5], it[3], it[6])} - .set{truth_ch} - - // Find TP_base (truth) - BCFTOOLS_ISEC_TRUTH( - truth_ch - ) - versions = versions.mix(BCFTOOLS_ISEC_TRUTH.out.versions) - - } - - if (params.method.contains('wittyer')){ - // - // MODULE: WITTYER - // - WITTYER( - input_ch, - bed, - [] - ) - versions = versions.mix(WITTYER.out.versions) - } - - - if (params.method.contains('vcfdist')){ - // - // MODULE: VCFDIST - // - VCFDIST( - input_ch, - ref, - bed - ) - versions = versions.mix(VCFDIST.out.versions) - } - - - emit: - versions -} diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 2c227a8..781660a 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -25,32 +25,30 @@ workflow INPUT_CHECK { def create_vcf_channel(LinkedHashMap row) { // create meta map def meta = [:] - meta.id = params.sample + meta.id = row.caller - def meta2 = [:] - meta2.caller = row.caller // add path(s) of the fastq file(s) to the meta map def vcf_meta = [] if (!file(row.test_vcf).exists()) { exit 1, "ERROR: Please check input samplesheet -> Test file does not exist!\n${row.test_vcf}" } - - if (meta2.caller == "delly"){ - vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/delly.yaml")] + + if (meta.id == "delly"){ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/delly.yaml")] } - else if (meta2.caller == "gridss"){ - vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/gridss.yaml")] + else if (meta.id == "gridss"){ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/gridss.yaml")] } - else if (meta2.caller == "manta"){ + else if (meta.id == "manta"){ if (file("${projectDir}/assets/svync/manta.yaml").exists()){ - vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/manta.yaml")] - } + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/manta.yaml")] + } + } + else if (meta.id == "smoove"){ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/smoove.yaml")] } - else if (meta2.caller == "smoove"){ - vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/smoove.yaml")] - } else{ - vcf_meta = [ meta, meta2, file(row.test_vcf), file("${projectDir}/assets/svync/default.yaml")] - } + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/default.yaml")] + } return vcf_meta } diff --git a/subworkflows/local/prepare_regions.nf b/subworkflows/local/prepare_regions.nf deleted file mode 100644 index 050cf10..0000000 --- a/subworkflows/local/prepare_regions.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// PREPARE_STRATIFICATIONS: SUBWORKFLOW TO PREPARE BED FILES - HIGH CONFIDENCE AND OTHER LEVELS -// - -params.options = [:] - -include { MAIN_CHROMS } from '../../modules/local/main_chroms.nf' addParams( options: params.options ) -include { EXTRACT_MAIN } from '../../modules/local/extract_main.nf' addParams( options: params.options ) - - -workflow PREPARE_REGIONS { - take: - ref // reference channel [ref.fa, ref.fa.fai] - high_conf - - main: - - versions=Channel.empty() - - ref.map { it -> tuple([id: it[0].baseName], it[1]) } - .set{fasta} - - // this is not working! - - // get contig file including only main chroms - MAIN_CHROMS( - fasta - ) - main_chroms = MAIN_CHROMS.out.sizes - versions = versions.mix(MAIN_CHROMS.out.versions) - - high_conf.map { it -> tuple([id: it[0].baseName], it[0]) } - .set{bed} - bed.view() - EXTRACT_MAIN( - bed - ) - chr_list = EXTRACT_MAIN.out.chr_list - - emit: - main_chroms - chr_list - versions -} diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index f01ef6e..7c4f5a3 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -19,10 +19,8 @@ include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TEST } from '../../modules/nf-c workflow PREPARE_VCFS_TEST { take: - input_ch // channel: [val(meta),val(meta2), vcf] + input_ch // channel: [val(meta),vcf, index] ref // reference channel [ref.fa, ref.fa.fai] - main_chroms // channel path(chrom sizes) - chr_list main: @@ -63,7 +61,7 @@ workflow PREPARE_VCFS_TEST { // // BCFTOOLS_NORM // - // Breaks down -any- multi-allelic variants + // Breaks down -any- multi-allelic variants BCFTOOLS_NORM_1( vcf_ch, ref, @@ -75,14 +73,13 @@ workflow PREPARE_VCFS_TEST { BCFTOOLS_NORM_1.out.vcf ) - BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:1) - .map{it -> tuple( it[1], it[0], it[2], it[4])} + BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) .set{vcf_ch} } if (params.min_sv_size > 0){ TABIX_BGZIP( - vcf_ch.map{it -> tuple( it[0], it[1], it[2])} + vcf_ch.map{it -> tuple( it[0], it[1])} ) versions = versions.mix(TABIX_BGZIP.out.versions) @@ -91,7 +88,7 @@ workflow PREPARE_VCFS_TEST { // // filters out smaller SVs than min_sv_size SURVIVOR_FILTER( - TABIX_BGZIP.out.output.map{it -> tuple( it[0], it[1], it[2], [])}, + TABIX_BGZIP.out.output.map{it -> tuple( it[0], it[1],[])}, params.min_sv_size, -1, -1, @@ -121,8 +118,7 @@ workflow PREPARE_VCFS_TEST { BCFTOOLS_NORM_2.out.vcf ) - BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:1) - .map{it -> tuple( it[1], it[0], it[2], it[4])} + BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) .set{vcf_ch} } emit: diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 10cff46..8396ab8 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -26,7 +26,7 @@ workflow PREPARE_VCFS_TRUTH { // // PREPARE_VCFS // - truth_ch.map { it -> tuple([id: params.sample],[caller:"truth"], it[0]) } + truth_ch.map { it -> tuple([id: "truth"], it[0]) } .set{truth} // BGZIP if needed and index truth @@ -53,7 +53,7 @@ workflow PREPARE_VCFS_TRUTH { // MODULE: BCFTOOLS_NORM // // Normalize test - // multi-allelic variants will be splitted. + // multi-allelic variants will be splitted. BCFTOOLS_NORM_1( vcf_ch, ref, @@ -66,8 +66,7 @@ workflow PREPARE_VCFS_TRUTH { ) versions = versions.mix(TABIX_TABIX_1.out.versions) - BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:1) - .map{it -> tuple(it[1],it[0], it[2], it[4])} + BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) .set{vcf_ch} } if (params.preprocess.contains("deduplication")){ @@ -87,8 +86,7 @@ workflow PREPARE_VCFS_TRUTH { ) versions = versions.mix(TABIX_TABIX_2.out.versions) - BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:1) - .map{it -> tuple(it[1],it[0], it[2], it[4])} + BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) .set{vcf_ch} } diff --git a/subworkflows/local/vcf_conversion.nf b/subworkflows/local/vcf_conversion.nf index b6e355f..c30bb6a 100644 --- a/subworkflows/local/vcf_conversion.nf +++ b/subworkflows/local/vcf_conversion.nf @@ -7,67 +7,58 @@ params.options = [:] include { MANTA_CONVERTINVERSION } from '../../modules/nf-core/manta/convertinversion' addParams( options: params.options ) include { GRIDSS_ANNOTATION } from '../../modules/local/gridss_annotation' addParams( options: params.options ) include { SVYNC } from '../../modules/nf-core/svync' addParams( options: params.options ) -include { AWK_SORT } from '../../modules/local/awk_sort.nf' addParams( options: params.options ) +include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) workflow VCF_CONVERSIONS { take: - input_ch // channel: [val(meta),val(meta2), vcf, config.yml] + input_ch // channel: [val(meta), vcf, config.yml] ref // reference channel [ref.fa, ref.fa.fai] main: - - out_vcf_ch = Channel.empty() versions = Channel.empty() // - // MODULE: AWK_SORT + // MODULE: BGZIP_TABIX // - // sort and index input test files - - AWK_SORT( - input_ch.map{it -> tuple(it[0], it[1], it[2])} - ) - versions = versions.mix(AWK_SORT.out.versions) - vcf_ch = AWK_SORT.out.vcf - + // zip and index input test files + + BGZIP_TABIX( + input_ch.map{it -> tuple(it[0], it[1])} + ) + versions = versions.mix(BGZIP_TABIX.out.versions) + vcf_ch = BGZIP_TABIX.out.gz_tbi + // // MODULE: SVYNC // - // - if(params.standardization){ - - vcf_ch.branch{ - tool: it[1].caller == "delly" || it[1].caller == "gridss" || it[1].caller == "manta" || it[1].caller == "smoove" - other: true} - .set{main_vcf_ch} - + // + if(params.standardization){ + out_vcf_ch = Channel.empty() - input_ch.map{it -> tuple(it[0], it[1], it[3])} - .combine(vcf_ch, by:1) - .map{it -> tuple(it[1], it[0], it[4], it[5], it[2])} + input_ch.map{it -> tuple(it[0], it[2])} + .combine(vcf_ch, by:0) .set{snd_ch} + snd_ch.view() snd_ch.branch{ - tool: it[1].caller == "delly" || it[1].caller == "gridss" || it[1].caller == "manta" || it[1].caller == "smoove" + tool: it[0].id == "delly" || it[0].id == "gridss" || it[0].id == "manta" || it[0].id == "smoove" other: true} .set{input} - + SVYNC( input.tool ) out_vcf_ch = out_vcf_ch.mix(SVYNC.out.vcf) out_vcf_ch = out_vcf_ch.mix(input.other) - }else{ - out_vcf_ch = vcf_ch + vcf_ch = out_vcf_ch } - - out2_vcf_ch = Channel.empty() // Check tool spesific conversions if(params.bnd_to_inv){ + out_vcf_ch = Channel.empty() out_vcf_ch.branch{ - tool: it[1].caller == "manta" || it[1].caller == "dragen" + tool: it[0].id == "manta" || it[0].id == "dragen" other: true} .set{input} // @@ -75,28 +66,26 @@ workflow VCF_CONVERSIONS { // //NOTE: should also work for dragen // Not working now!!!!! - + MANTA_CONVERTINVERSION( input.tool, ref ) versions = versions.mix(MANTA_CONVERTINVERSION.out.versions) - out2_vcf_ch = out2_vcf_ch.mix(MANTA_CONVERTINVERSION.out.vcf_tabi) - out2_vcf_ch = out2_vcf_ch.mix(input.other) + out_vcf_ch = out_vcf_ch.mix(MANTA_CONVERTINVERSION.out.vcf_tabi) + out_vcf_ch = out_vcf_ch.mix(input.other) + vcf_ch = out_vcf_ch // https://github.com/srbehera/DRAGEN_Analysis/blob/main/convertInversion.py } - else{ - out2_vcf_ch = out_vcf_ch - } - - out3_vcf_ch = Channel.empty() if (params.gridss_annotate){ + out_vcf_ch = Channel.empty() + out2_vcf_ch.branch{ - tool: it[1].caller == "gridss" + tool: it[0].id == "gridss" other: true} .set{input} @@ -111,16 +100,13 @@ workflow VCF_CONVERSIONS { ) versions = versions.mix(GRIDSS_ANNOTATION.out.versions) - out3_vcf_ch = out3_vcf_ch.mix(GRIDSS_ANNOTATION.out.vcf) - out3_vcf_ch = out3_vcf_ch.mix(input.other) - } - else{ - out3_vcf_ch = out2_vcf_ch + out_vcf_ch = out_vcf_ch.mix(GRIDSS_ANNOTATION.out.vcf) + out_vcf_ch = out_vcf_ch.mix(input.other) + vcf_ch = out_vcf_ch } - // https://github.com/EUCANCan/variant-extractor/blob/main/examples/vcf_to_csv.py emit: - out3_vcf_ch + vcf_ch versions } diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 6ec48fe..0819bfc 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -53,7 +53,6 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil include { INPUT_CHECK } from '../subworkflows/local/input_check' include { SOMATIC_BENCHMARK } from '../subworkflows/local/somatic_benchmark' include { GERMLINE_BENCHMARK } from '../subworkflows/local/germline_benchmark' -include { PREPARE_REGIONS } from '../subworkflows/local/prepare_regions' include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' include { VCF_CONVERSIONS } from '../subworkflows/local/vcf_conversion' @@ -95,15 +94,6 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) ch_input = INPUT_CHECK.out.ch_sample - // - // PREPARE_REGIONS: prepare stratifications and contigs - // - PREPARE_REGIONS( - ref, - high_conf - ) - ch_versions = ch_versions.mix(PREPARE_REGIONS.out.versions) - // // SUBWORKFLOW: VCF_CONVERSIONS // @@ -124,10 +114,8 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) PREPARE_VCFS_TEST( - VCF_CONVERSIONS.out.out3_vcf_ch.map{it -> tuple(it[0], it[1], it[2], it[3])}, - ref, - PREPARE_REGIONS.out.main_chroms, - PREPARE_REGIONS.out.chr_list + VCF_CONVERSIONS.out.vcf_ch, + ref ) ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) @@ -145,13 +133,8 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) // prepare benchmark set - - high_conf.map { it -> tuple([id: params.sample],[caller:"truth"], it[0]) } - .set{bed} - - PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch, by:0) - .combine(bed, by:0) - .map{it -> tuple(it[0],it[1], it[2], it[3], it[5], it[6], it[8])} + PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) + .combine(high_conf) .set{bench_ch} // From e7dbb9de1802bfd6ad89c17f7dbe3b54f6e3534a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 20 Feb 2024 16:22:01 +0100 Subject: [PATCH 014/292] test with full --- assets/samplesheet.csv | 6 ++-- assets/samplesheet_HG002.csv | 5 ++-- assets/samplesheet_HG002_hg38.csv | 2 +- assets/schema_input.json | 2 +- conf/test.config | 22 +++++++++----- modules/local/addhead.nf | 38 ------------------------ modules/local/extract_main.nf | 33 -------------------- modules/local/main_chroms.nf | 34 --------------------- modules/nf-core/svync/main.nf | 2 +- nextflow.config | 4 +-- nextflow_schema.json | 2 +- subworkflows/local/germline_benchmark.nf | 8 ++--- subworkflows/local/vcf_conversion.nf | 5 ++-- workflows/variantbenchmarking.nf | 18 +++++++---- 14 files changed, 46 insertions(+), 135 deletions(-) delete mode 100644 modules/local/addhead.nf delete mode 100644 modules/local/extract_main.nf delete mode 100644 modules/local/main_chroms.nf diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index d7db7d3..25236ed 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,3 @@ -sample,test_vcf,truth_vcf,caller,type -HG002,"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/HG002.svaba.germline.sv.convBNDtoDEL.vcf","/Users/w620-admin/Desktop/nf-core/dataset/hg37/GIAB_Assemblytics_structural_variants_only_160618/hg002.Assemblytics_structural_variants.sorted.vcf.gz",svaba,sv -HG003,"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/HG003.svaba.germline.sv.convBNDtoDEL.vcf","/Users/w620-admin/Desktop/nf-core/dataset/hg37/GIAB_Assemblytics_structural_variants_only_160618/hg003.Assemblytics_structural_variants.sorted.vcf.gz",svaba,sv +test_vcf,caller +"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",mutect +"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz",unknown diff --git a/assets/samplesheet_HG002.csv b/assets/samplesheet_HG002.csv index db4ccca..9ff5a9e 100644 --- a/assets/samplesheet_HG002.csv +++ b/assets/samplesheet_HG002.csv @@ -1,4 +1,5 @@ test_vcf,caller "/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_delly_SV_hg19.vcf.gz",delly -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.vcf.gz",lumpy -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype.vcf",manta \ No newline at end of file +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.sorted.vcf.gz",lumpy +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype2.vcf",manta +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/full.svaba.germline.sv.vcf",svaba diff --git a/assets/samplesheet_HG002_hg38.csv b/assets/samplesheet_HG002_hg38.csv index 95cbeef..cc0fa08 100644 --- a/assets/samplesheet_HG002_hg38.csv +++ b/assets/samplesheet_HG002_hg38.csv @@ -1,5 +1,5 @@ test_vcf,caller "/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.vcf.gz",lumpy "/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/manta.HG002.vcf.gz",manta -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Ashkenazim_unnanotated/Ashkenazim_HG002.filtered.sv.vcf.gz",merged +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/Ashkenazim_unnanotated/Ashkenazim_HG002.filtered.sv.vcf.gz",merged diff --git a/assets/schema_input.json b/assets/schema_input.json index 2ef1310..b919404 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,5 +1,5 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/assets/schema_input.json", "title": "nf-core/variantbenchmarking pipeline - params.input schema", "description": "Schema for the file provided with params.input", diff --git a/conf/test.config b/conf/test.config index 3150c35..b2c3ec8 100644 --- a/conf/test.config +++ b/conf/test.config @@ -15,15 +15,23 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.GB' - max_time = '6.h' + max_cpus = 16 + max_memory = '100.GB' + max_time = '8.h' // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + sample = "HG002" + input = 'assets/samplesheet.csv' // Genome references - genome = 'R64-1-1' + genome = 'hg38' + + // Processes + analysis = 'germline' + method = 'truvari,svanalyzer,wittyer' // --not working for now : vcfdist + similarity = 0 // determines the sequence similarity level in benchmarking. + preprocess = "normalization, deduplication" + min_sv_size = 50 + + truth = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" } diff --git a/modules/local/addhead.nf b/modules/local/addhead.nf deleted file mode 100644 index 89a2a50..0000000 --- a/modules/local/addhead.nf +++ /dev/null @@ -1,38 +0,0 @@ -process ADDHEAD { - tag "$meta.id $meta2.caller" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" - - input: - tuple val(meta), val(meta2), path(vcf), path(header) - - output: - tuple val(meta), val(meta2), path("*.vcf.gz") , emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def prefix = task.ext.prefix ?: "${meta.id}" - - """ - cat $header $vcf > ${prefix}_rehead.vcf - - bcftools \\ - sort \\ - --output ${prefix}.vcf.gz \\ - --output-type z \\ - --temp-dir . \\ - ${prefix}_rehead.vcf - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/local/extract_main.nf b/modules/local/extract_main.nf deleted file mode 100644 index 819b077..0000000 --- a/modules/local/extract_main.nf +++ /dev/null @@ -1,33 +0,0 @@ -process EXTRACT_MAIN { - tag "$meta.id" - label 'process_single' - - conda "" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" - - input: - tuple val(meta), path(bed) - - output: - tuple val(meta), path("*.txt"), emit: chr_list - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def name = bed.getBaseName() - - """ - cat $bed | awk '{print \$1}' | uniq > ${name}.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') - END_VERSIONS - """ - -} diff --git a/modules/local/main_chroms.nf b/modules/local/main_chroms.nf deleted file mode 100644 index 3078ef1..0000000 --- a/modules/local/main_chroms.nf +++ /dev/null @@ -1,34 +0,0 @@ -process MAIN_CHROMS { - tag "$meta.id" - label 'process_single' - - conda "" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" - - input: - tuple val(meta), path(fai) - - output: - tuple val(meta), path("*.sizes"), emit: sizes - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def name = fai.getBaseName() - - """ - cut -f 1,2 $fai > size - head -n24 size > ${name}.sizes - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') - END_VERSIONS - """ - -} diff --git a/modules/nf-core/svync/main.nf b/modules/nf-core/svync/main.nf index 66c2f1a..8c82a92 100644 --- a/modules/nf-core/svync/main.nf +++ b/modules/nf-core/svync/main.nf @@ -12,7 +12,7 @@ process SVYNC { output: tuple val(meta),path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: vcf - path "versions.yml" , emit: versions + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/nextflow.config b/nextflow.config index 1f4f8d7..dff170d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -199,10 +199,10 @@ profiles { executor.cpus = 4 executor.memory = 8.GB } + test { includeConfig 'conf/test.config' } test_hg37 { includeConfig 'conf/test_hg37.config' } test_hg38 { includeConfig 'conf/test_hg38.config' } - test_full { includeConfig 'conf/test_full.config' } - truth { includeConfig 'conf/truth.config' } + test_full { includeConfig 'conf/test_full.config' } } diff --git a/nextflow_schema.json b/nextflow_schema.json index 9cb22d3..fd64288 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,5 +1,5 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", "title": "nf-core/variantbenchmarking pipeline parameters", "description": "Master benchmarking pipeline for variant callers ", diff --git a/subworkflows/local/germline_benchmark.nf b/subworkflows/local/germline_benchmark.nf index e07f1a6..2e3319a 100644 --- a/subworkflows/local/germline_benchmark.nf +++ b/subworkflows/local/germline_benchmark.nf @@ -13,9 +13,8 @@ include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon_evaluato workflow GERMLINE_BENCHMARK { take: - input_ch // channel: [val(meta),val(meta2), test_vcf, test_index , truth_vcf, truth_index] + input_ch // channel: [val(meta),test_vcf,test_index,truth_vcf,truth_index, bed] ref // reference channel [ref.fa, ref.fa.fai] - truth_vcf // channel: [val(meta),val(meta2),truth_vcf, truth_index] main: @@ -42,7 +41,7 @@ workflow GERMLINE_BENCHMARK { ref ) versions = versions.mix(TRUVARI_BENCH.out.versions) - + } if (params.method.contains('svanalyzer')){ @@ -56,7 +55,7 @@ workflow GERMLINE_BENCHMARK { ) versions = versions.mix(SVANALYZER_SVBENCHMARK.out.versions) - } + } if (params.method.contains('wittyer')){ // @@ -71,7 +70,6 @@ workflow GERMLINE_BENCHMARK { versions = versions.mix(WITTYER.out.versions) } - if (params.method.contains('vcfdist')){ // // MODULE: VCFDIST diff --git a/subworkflows/local/vcf_conversion.nf b/subworkflows/local/vcf_conversion.nf index c30bb6a..ae9827d 100644 --- a/subworkflows/local/vcf_conversion.nf +++ b/subworkflows/local/vcf_conversion.nf @@ -7,7 +7,7 @@ params.options = [:] include { MANTA_CONVERTINVERSION } from '../../modules/nf-core/manta/convertinversion' addParams( options: params.options ) include { GRIDSS_ANNOTATION } from '../../modules/local/gridss_annotation' addParams( options: params.options ) include { SVYNC } from '../../modules/nf-core/svync' addParams( options: params.options ) -include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) +include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) workflow VCF_CONVERSIONS { take: @@ -37,6 +37,7 @@ workflow VCF_CONVERSIONS { input_ch.map{it -> tuple(it[0], it[2])} .combine(vcf_ch, by:0) + .map{it -> tuple(it[0], it[2], it[3], it[1])} .set{snd_ch} snd_ch.view() @@ -50,7 +51,7 @@ workflow VCF_CONVERSIONS { ) out_vcf_ch = out_vcf_ch.mix(SVYNC.out.vcf) out_vcf_ch = out_vcf_ch.mix(input.other) - vcf_ch = out_vcf_ch + vcf_ch = out_vcf_ch.map{it -> tuple(it[0], it[1], it[2])} } // Check tool spesific conversions diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 0819bfc..20aa190 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -94,6 +94,8 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) ch_input = INPUT_CHECK.out.ch_sample + // TODO: SMALL VARIANT BENCHMARKING + // // SUBWORKFLOW: VCF_CONVERSIONS // @@ -133,9 +135,15 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) // prepare benchmark set - PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) + if (params.high_conf){ + PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) .combine(high_conf) .set{bench_ch} + }else{ + PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) + .map{it -> tuple(it[0], it[1], it[2],it[3],it[4],[])} + .set{bench_ch} + } // // SUBWORKFLOW: GERMLINE_BENCHMARK @@ -144,12 +152,10 @@ workflow VARIANTBENCHMARKING { GERMLINE_BENCHMARK( bench_ch, - ref, - PREPARE_VCFS_TRUTH.out.vcf_ch - ) + ref ) ch_versions = ch_versions.mix(GERMLINE_BENCHMARK.out.versions) - + // TODO: SOMATIC EBNCHMARKING if (params.analysis.contains("somatic")){ // SOMATIC VARIANT BENCHMARKING @@ -168,6 +174,8 @@ workflow VARIANTBENCHMARKING { // TODO: TRIO ANALYSIS : MENDELIAN INCONSISTANCE + // TODO: Compare benchmarking methods! + CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') ) From 4c397c80e3e8e35491fc2d3601d2640f355417dd Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:30:58 +0000 Subject: [PATCH 015/292] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- LICENSE | 2 +- README.md | 7 +- assets/email_template.html | 2 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 77 --- lib/WorkflowVariantbenchmarking.groovy | 122 ----- main.nf | 100 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 9 +- nextflow_schema.json | 3 +- pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../main.nf | 247 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/variantbenchmarking.nf | 133 ++---- 63 files changed, 2114 insertions(+), 1412 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowVariantbenchmarking.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b99008..dd9ffa5 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index ae0bf81..11820a8 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 8c2110e..f9e9e20 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index f6fed81..58404c3 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 9008798..4724054 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda..0b6b1f2 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458..f823210 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098..748b431 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd1..b706875 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f0..c3674af 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/LICENSE b/LICENSE index e48cb19..0aefc2e 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) Kübra Narcı +Copyright (c) kuebra.narci@dkfz.de Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index d9d4aaa..e82ef33 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/variantbenchmarking -[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -78,7 +79,7 @@ For more details about the output files and reports, please refer to the ## Credits -nf-core/variantbenchmarking was originally written by Kübra Narcı. +nf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de. We thank the following people for their extensive assistance in the development of this pipeline: diff --git a/assets/email_template.html b/assets/email_template.html index ee343c4..afaac61 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + nf-core/variantbenchmarking Pipeline Report diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index d26a71b..9b0ed73 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index c4f8dd9..19da518 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe..0000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6ab..e3ea8fa 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c..0000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4..0000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 025eed8..0000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,77 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/variantbenchmarking pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowVariantbenchmarking.groovy b/lib/WorkflowVariantbenchmarking.groovy deleted file mode 100755 index ae9238e..0000000 --- a/lib/WorkflowVariantbenchmarking.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/variantbenchmarking.nf in the nf-core/variantbenchmarking pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowVariantbenchmarking { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/main.nf b/main.nf index 175c581..3633aec 100644 --- a/main.nf +++ b/main.nf @@ -13,66 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +include { VARIANTBENCHMARKING } from './workflows/variantbenchmarking' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { VARIANTBENCHMARKING } from './workflows/variantbenchmarking' - // -// WORKFLOW: Run main nf-core/variantbenchmarking analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_VARIANTBENCHMARKING { - VARIANTBENCHMARKING () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + VARIANTBENCHMARKING ( + samplesheet + ) + + emit: + multiqc_report = VARIANTBENCHMARKING.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_VARIANTBENCHMARKING () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_VARIANTBENCHMARKING ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_VARIANTBENCHMARKING.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index 789c505..c536282 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index 87d88f4..0000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/variantbenchmarking/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272b..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f218761..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5f..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d4..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - - - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a93..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24..0000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c66..70edae4 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb..86f7c31 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b75..2212096 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c4..354f443 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438ed..f1c4242 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e730..c204b48 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index b46c50e..fc0b062 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten @@ -229,9 +226,9 @@ dag { manifest { name = 'nf-core/variantbenchmarking' - author = """Kübra Narcı""" + author = """kuebra.narci@dkfz.de""" homePage = 'https://github.com/nf-core/variantbenchmarking' - description = """Nextflow variant benchmarking pipeline""" + description = """Master benchmarking pipeline for Structural Variant callers """ mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '1.0dev' diff --git a/nextflow_schema.json b/nextflow_schema.json index 861d2ab..0042971 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -2,7 +2,7 @@ "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", "title": "nf-core/variantbenchmarking pipeline parameters", - "description": "Nextflow variant benchmarking pipeline", + "description": "Master benchmarking pipeline for Structural Variant callers ", "type": "object", "definitions": { "input_output_options": { @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c..5611062 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87..0000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf new file mode 100644 index 0000000..dc90ad9 --- /dev/null +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -0,0 +1,247 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +}// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 0000000..ac31f28 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 0000000..e5c3a0a --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..8ed4310 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..db2030f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..f7c54bc --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 0000000..53574ff --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 0000000..f847611 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 0000000..a8b55d6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 0000000..d08d243 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 0000000..1dc317f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 0000000..10f948e --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 0000000..8940d32 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 0000000..d07ce54 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 0000000..d0a926b --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 0000000..ac8523c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 0000000..2585b65 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 0000000..3d4a6b0 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 0000000..517ee54 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 0000000..7626c1c --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 0000000..60b1cff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 04a3bf2..90940f1 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowVariantbenchmarking.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow VARIANTBENCHMARKING { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowVariantbenchmarking.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowVariantbenchmarking.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow VARIANTBENCHMARKING { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From 102dd357f721c4d22a25ac0359d231e12c399c84 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 22 Feb 2024 11:22:08 +0100 Subject: [PATCH 016/292] vartype added --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index fd64288..9cb22d3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,5 +1,5 @@ { - "$schema": "https://json-schema.org/draft/2020-12/schema", + "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", "title": "nf-core/variantbenchmarking pipeline parameters", "description": "Master benchmarking pipeline for variant callers ", From 8b59477df3bb649fb65385cea0ee1e19186df3e8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 22 Feb 2024 11:22:20 +0100 Subject: [PATCH 017/292] vartype added --- assets/samplesheet.csv | 6 +- assets/samplesheet_HG002.csv | 10 +- assets/samplesheet_HG002_hg19.csv | 5 - assets/samplesheet_HG002_hg38.csv | 10 +- assets/schema_input.json | 10 +- bin/GangSTRConvertTruvariCompatible.py | 166 ++++++++++++++++++ bin/check_samplesheet.py | 22 +-- conf/modules.config | 2 +- subworkflows/local/input_check.nf | 37 ++-- subworkflows/local/prepare_vcfs_test.nf | 36 ++-- subworkflows/local/report_vcf_statistics.nf | 10 +- ..._benchmark.nf => sv_germline_benchmark.nf} | 4 +- ...vcf_conversion.nf => sv_vcf_conversion.nf} | 5 +- workflows/variantbenchmarking.nf | 59 +++++-- 14 files changed, 300 insertions(+), 82 deletions(-) delete mode 100644 assets/samplesheet_HG002_hg19.csv create mode 100644 bin/GangSTRConvertTruvariCompatible.py rename subworkflows/local/{germline_benchmark.nf => sv_germline_benchmark.nf} (96%) rename subworkflows/local/{vcf_conversion.nf => sv_vcf_conversion.nf} (96%) diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 25236ed..2268766 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,3 @@ -test_vcf,caller -"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",mutect -"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz",unknown +test_vcf,caller,vartype +"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",mutect,sv +"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz",unknown,sv diff --git a/assets/samplesheet_HG002.csv b/assets/samplesheet_HG002.csv index 9ff5a9e..10f67ed 100644 --- a/assets/samplesheet_HG002.csv +++ b/assets/samplesheet_HG002.csv @@ -1,5 +1,5 @@ -test_vcf,caller -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_delly_SV_hg19.vcf.gz",delly -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.sorted.vcf.gz",lumpy -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype2.vcf",manta -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/full.svaba.germline.sv.vcf",svaba +test_vcf,caller,vartype +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_delly_SV_hg19.vcf.gz",delly,sv +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.sorted.vcf.gz",lumpy,sv +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype2.vcf",manta,sv +"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/full.svaba.germline.sv.vcf",svaba,sv diff --git a/assets/samplesheet_HG002_hg19.csv b/assets/samplesheet_HG002_hg19.csv deleted file mode 100644 index d1d8dba..0000000 --- a/assets/samplesheet_HG002_hg19.csv +++ /dev/null @@ -1,5 +0,0 @@ -test_vcf,caller -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_DRAGEN_SV_hg19.vcf.gz",dragen -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_delly_SV_hg19.vcf.gz",delly -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.vcf.gz",lumpy -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype.vcf",manta \ No newline at end of file diff --git a/assets/samplesheet_HG002_hg38.csv b/assets/samplesheet_HG002_hg38.csv index cc0fa08..06171dc 100644 --- a/assets/samplesheet_HG002_hg38.csv +++ b/assets/samplesheet_HG002_hg38.csv @@ -1,5 +1,7 @@ -test_vcf,caller -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.vcf.gz",lumpy -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/manta.HG002.vcf.gz",manta -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/Ashkenazim_unnanotated/Ashkenazim_HG002.filtered.sv.vcf.gz",merged +test_vcf,caller,vartype +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.vcf.gz",lumpy,sv +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/manta.HG002.vcf.gz",manta,sv +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/Ashkenazim_unnanotated/Ashkenazim_HG002.filtered.sv.vcf.gz",merged,sv +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_DRAGEN_SV_hg19.vcf.gz",dragen,sv + diff --git a/assets/schema_input.json b/assets/schema_input.json index b919404..ee44386 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,5 +1,5 @@ { - "$schema": "https://json-schema.org/draft/2020-12/schema", + "$schema": "http://json-schema.org/draft-07/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/assets/schema_input.json", "title": "nf-core/variantbenchmarking pipeline - params.input schema", "description": "Schema for the file provided with params.input", @@ -16,8 +16,14 @@ "type": "string", "pattern": "^\\S+$", "errorMessage": "Name of the variant caller used to generate test file" + }, + "vartype": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Variant type to benchmark" } + }, - "required": ["test_vcf","caller"] + "required": ["test_vcf","caller","vartype"] } } diff --git a/bin/GangSTRConvertTruvariCompatible.py b/bin/GangSTRConvertTruvariCompatible.py new file mode 100644 index 0000000..3ca6950 --- /dev/null +++ b/bin/GangSTRConvertTruvariCompatible.py @@ -0,0 +1,166 @@ +import argparse +import re +from Bio import SeqIO + +header = """##FILTER= +##INFO= +##INFO= +##INFO= +##EVENTTYPE= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##INFO= +##FORMAT= +##FORMAT= +##ALT= +""" + + +def translateLine(line: str, ref: dict) -> str: + lineSplit = line.strip("\n").split("\t") + + contigsAndAlleles = lineSplit[:-3] + contigsAndAlleles[3] = ref[contigsAndAlleles[0]].seq[ + int(contigsAndAlleles[1]) - 1 + ] + contigsAndAlleles[4] = re.sub("[a-z]+", "", contigsAndAlleles[4]) + + infoField = lineSplit[-3] + infoDict = re.findall("([A-Z]+)=([\+\-A-Za-z\.\d_:]+);", infoField) + assert len(infoDict) != 0 + infoDict = dict(infoDict) + + contigsAndAlleles[2] = ( + "tr_" + + contigsAndAlleles[0] + + "_" + + contigsAndAlleles[1] + + "_" + + infoDict["END"] + ) + + formatKeys = lineSplit[-2].split(":") + if lineSplit[-1] == ".": + formatValues = ["."] * len(formatKeys) + else: + formatValues = lineSplit[-1].split(":") + formatDict = dict(zip(formatKeys, formatValues)) + + rul = len(infoDict["RU"]) + svlen = int(infoDict["END"]) - int(contigsAndAlleles[1]) + refruc = float(svlen) / rul + svlen = str(svlen) + repcn = formatDict["REPCN"].split(",") + + if formatDict["GT"] in ["./.", "."]: + cn = "." + ruc = "." + rucchange = "." + cnvtrlen = "." + cnformat = "." + else: + if formatDict["GT"] == "1/2": + svlen = [svlen] * 2 + ruc = [float(x) for x in repcn] + cn = [x / refruc for x in ruc] + rucchange = [x - refruc for x in ruc] + cnvtrlen = [x * rul for x in rucchange] + else: + svlen = [svlen] + ruc = ( + float(repcn[0]) + if formatDict["GT"] == "1/0" + else float(repcn[-1]) + ) + cn = [ruc / refruc] + rucchange = [ruc - refruc] + cnvtrlen = [rucchange[0] * rul] + ruc = [ruc] + cnformat = f"{sum(cn):.6f}" + svlen = ",".join(svlen) + cn = ",".join([f"{x:.2f}" for x in cn]) + ruc = ",".join([f"{x:.2f}" for x in ruc]) + rucchange = ",".join([f"{x:.6f}" for x in rucchange]) + cnvtrlen = ",".join([f"{x:.6f}" for x in cnvtrlen]) + + info = ["SVTYPE=CNV", "EVENTTYPE=STR"] + + info.append(f"SVLEN={svlen}") + info.append(f"END={infoDict['END']}") + info.append(f"REFRUC={refruc:.2f}") + info.append(f"RUS={infoDict['RU'].upper()}") + # RUL Gives issues with Wittyer (RulMismatch) not sure why + # info.append( + # f"RUL={len(infoDict['RU'])}" + # ) + info.append(f"CN={cn}") + info.append(f"RUC={ruc}") + info.append(f"RUCCHANGE={rucchange}") + info.append(f"CNVTRLEN={cnvtrlen}") + + info = ";".join(info) + + formatFields = ":".join(["GT", "CN"]) + + gt = formatDict["GT"] + gt = "0/1" if gt == "1/0" else gt + + format = ":".join([gt, cnformat]) + + """ + HOMREF calls have to be "masked" when using Witty.er, otherwise a HOMREF call that is not + present in the truth will be counted as a query FP, decreasing precision for no reason. + It's sufficient to change the FILTER from PASS or . to something else (like HOMREF) + """ + contigsAndAlleles[6] = "HOMREF" if gt in ["0/0", "0"] else "PASS" + + contigsAndAlleles = "\t".join(contigsAndAlleles) + return "\t".join([contigsAndAlleles, info, formatFields, format]) + "\n" + + +def convertVcf(inputFile: str, outputFile: str, reference: str) -> None: + inputHandle = open(inputFile, "r") + outputHandle = open(outputFile, "w") + + referenceDict = SeqIO.to_dict(SeqIO.parse(reference, "fasta")) + + for line in inputHandle: + if re.search("^#[^#]", line): + outputHandle.write(header) + outputHandle.write(line) + continue + + if re.search("^##(INFO)|(FORMAT)|(ALT)", line): + continue + + if re.search("^##", line): + outputHandle.write(line) + continue + + outputHandle.write(translateLine(line, referenceDict)) + + inputHandle.close() + outputHandle.close() + + +if __name__ == "__main__": + parser = argparse.ArgumentParser( + description="Convert the repeat genotyping module VCF output in a VCFv4.4 compliant format (similar to the VNTR module output)" + ) + parser.add_argument( + "-i", "--input", help="input VCF", type=str, required=True + ) + parser.add_argument( + "-r", "--reference", help="Genome FASTA", type=str, required=False + ) + parser.add_argument( + "-o", "--output", help="output VCF", type=str, required=True + ) + args = parser.parse_args() + + convertVcf(args.input, args.output, args.reference) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 5bbe208..b091701 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -36,9 +36,11 @@ def print_error(error, context="Line", context_str=""): def check_samplesheet(file_in, file_out): """ This function checks that the samplesheet follows the following structure: - test_vcf,caller - test1.vcf,manta - test2.vcf,svaba + test_vcf,caller,vartype + test1.vcf,manta,sv + test2.vcf,svaba,small + test3.vcf,cnvkit,cnv + For an example see: https://github.com/ghga-de/nf-benchmark/assets/samplesheet.csv """ @@ -47,8 +49,8 @@ def check_samplesheet(file_in, file_out): with open(file_in, "r", encoding='utf-8-sig') as fin: ## Check header - MIN_COLS = 2 - HEADER = ["test_vcf","caller"] + MIN_COLS = 3 + HEADER = ["test_vcf","caller","vartype"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print( @@ -78,7 +80,7 @@ def check_samplesheet(file_in, file_out): ) ## Check caller name entries - test_vcf, caller = lspl[: len(HEADER)] + test_vcf, caller, vartype = lspl[: len(HEADER)] if caller.find(" ") != -1: print( f"WARNING: Spaces have been replaced by underscores for caller: {caller}" @@ -87,11 +89,11 @@ def check_samplesheet(file_in, file_out): if not caller: print_error("Caller entry has not been specified!", "Line", line) - sample_info = [] ## [test_vcf, caller ] + sample_info = [] ## [test_vcf, caller, vartype ] - sample_info = [test_vcf, caller] + sample_info = [test_vcf, caller, vartype] - ## Create caller mapping dictionary = {caller: [[test_vcf, caller ]]} + ## Create caller mapping dictionary = {caller: [[test_vcf, caller, vartype ]]} if caller not in sample_mapping_dict: sample_mapping_dict[caller] = [sample_info] else: @@ -106,7 +108,7 @@ def check_samplesheet(file_in, file_out): make_dir(out_dir) with open(file_out, "w") as fout: fout.write( - ",".join(["test_vcf","caller"]) + ",".join(["test_vcf","caller", "vartype"]) + "\n" ) for caller in sorted(sample_mapping_dict.keys()): diff --git a/conf/modules.config b/conf/modules.config index 3711745..da5372a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -131,7 +131,7 @@ process { } withName: "TRUVARI_BENCH" { ext.prefix = {"${params.sample}"} - ext.args = {"--pctsize 0.5 --pctovl 0.5 --refdist 1000"} + ext.args = {"--pctsize 0.5 --pctovl 0.5 --refdist 1000 --pick ac"} publishDir = [ path: {"${params.outdir}/${meta.id}/truvari_bench"}, pattern: "*{.vcf.gz,vcf.gz.tbi,json}", diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 781660a..ebe0aff 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -26,29 +26,34 @@ def create_vcf_channel(LinkedHashMap row) { // create meta map def meta = [:] meta.id = row.caller + meta.vartype = row.vartype // add path(s) of the fastq file(s) to the meta map def vcf_meta = [] if (!file(row.test_vcf).exists()) { exit 1, "ERROR: Please check input samplesheet -> Test file does not exist!\n${row.test_vcf}" } - - if (meta.id == "delly"){ - vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/delly.yaml")] - } - else if (meta.id == "gridss"){ - vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/gridss.yaml")] - } - else if (meta.id == "manta"){ - if (file("${projectDir}/assets/svync/manta.yaml").exists()){ - vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/manta.yaml")] + if (meta.vartype == "sv"){ + if (meta.id == "delly"){ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/delly.yaml")] } + else if (meta.id == "gridss"){ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/gridss.yaml")] + } + else if (meta.id == "manta"){ + if (file("${projectDir}/assets/svync/manta.yaml").exists()){ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/manta.yaml")] + } + } + else if (meta.id == "smoove"){ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/smoove.yaml")] + } + else{ + vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/default.yaml")] + } + }else{ + vcf_meta = [ meta, file(row.test_vcf)] } - else if (meta.id == "smoove"){ - vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/smoove.yaml")] - } - else{ - vcf_meta = [ meta, file(row.test_vcf), file("${projectDir}/assets/svync/default.yaml")] - } + return vcf_meta } diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 7c4f5a3..5998a65 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -4,22 +4,23 @@ params.options = [:] -include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) -include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) -include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_1 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_2 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_3 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) +include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) +include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_1 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_2 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_3 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TEST } from '../../modules/nf-core/bcftools/reheader' addParams( options: params.options ) workflow PREPARE_VCFS_TEST { take: - input_ch // channel: [val(meta),vcf, index] + input_ch // channel: [val(meta),vcf] ref // reference channel [ref.fa, ref.fa.fai] main: @@ -28,12 +29,23 @@ workflow PREPARE_VCFS_TEST { //ref.map { it -> tuple([id: it[0].baseName], it[1]) } // .set{fasta} + + // + // MODULE: BGZIP_TABIX + // + // zip and index input test files + BGZIP_TABIX( + input_ch + ) + versions = versions.mix(BGZIP_TABIX.out.versions) + vcf_ch = BGZIP_TABIX.out.gz_tbi + // // PREPARE_VCFS // // Reheader needed to standardize sample names BCFTOOLS_REHEADER_TEST( - input_ch, + vcf_ch, ref ) versions = versions.mix(BCFTOOLS_REHEADER_TEST.out.versions) diff --git a/subworkflows/local/report_vcf_statistics.nf b/subworkflows/local/report_vcf_statistics.nf index a17a262..4828408 100644 --- a/subworkflows/local/report_vcf_statistics.nf +++ b/subworkflows/local/report_vcf_statistics.nf @@ -15,12 +15,16 @@ workflow REPORT_VCF_STATISTICS { versions=Channel.empty() + input_ch.branch{ + sv: it[0].vartype == "sv" || it[0].vartype == "cnv" + other: true} + .set{input} + // // SURVIVOR_STATS // - SURVIVOR_STATS( - input_ch, + input.sv, -1, -1, -1 @@ -43,7 +47,7 @@ workflow REPORT_VCF_STATISTICS { versions = versions.mix(BCFTOOLS_STATS.out.versions) - // Add here a tool, to visualize SV statistics in a histogram. + // Add here a tool, to visualize SV statistics in a histogram. emit: bcftools_stats diff --git a/subworkflows/local/germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf similarity index 96% rename from subworkflows/local/germline_benchmark.nf rename to subworkflows/local/sv_germline_benchmark.nf index 2e3319a..2e4cc0c 100644 --- a/subworkflows/local/germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -1,5 +1,5 @@ // -// GERMLINE: SUBWORKFLOW FOR GERMLINE VARIANTS +// SV_GERMLINE_BENCHMARK: SUBWORKFLOW FOR SV GERMLINE VARIANTS // params.options = [:] @@ -11,7 +11,7 @@ include { WITTYER } from '../../modules/local/wittyer' include { VCFDIST } from '../../modules/local/vcfdist' addParams( options: params.options ) include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon_evaluator' addParams( options: params.options ) -workflow GERMLINE_BENCHMARK { +workflow SV_GERMLINE_BENCHMARK { take: input_ch // channel: [val(meta),test_vcf,test_index,truth_vcf,truth_index, bed] ref // reference channel [ref.fa, ref.fa.fai] diff --git a/subworkflows/local/vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf similarity index 96% rename from subworkflows/local/vcf_conversion.nf rename to subworkflows/local/sv_vcf_conversion.nf index ae9827d..18e5ae9 100644 --- a/subworkflows/local/vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -1,5 +1,5 @@ // -// VCF_CONVERSIONS: SUBWORKFLOW TO apply tool spesific conversions +// SV_VCF_CONVERSIONS: SUBWORKFLOW TO apply tool spesific conversions // params.options = [:] @@ -9,7 +9,7 @@ include { GRIDSS_ANNOTATION } from '../../modules/local/gridss_annotation' include { SVYNC } from '../../modules/nf-core/svync' addParams( options: params.options ) include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) -workflow VCF_CONVERSIONS { +workflow SV_VCF_CONVERSIONS { take: input_ch // channel: [val(meta), vcf, config.yml] ref // reference channel [ref.fa, ref.fa.fai] @@ -40,7 +40,6 @@ workflow VCF_CONVERSIONS { .map{it -> tuple(it[0], it[2], it[3], it[1])} .set{snd_ch} - snd_ch.view() snd_ch.branch{ tool: it[0].id == "delly" || it[0].id == "gridss" || it[0].id == "manta" || it[0].id == "smoove" other: true} diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 20aa190..a02f4f9 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -52,10 +52,10 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // include { INPUT_CHECK } from '../subworkflows/local/input_check' include { SOMATIC_BENCHMARK } from '../subworkflows/local/somatic_benchmark' -include { GERMLINE_BENCHMARK } from '../subworkflows/local/germline_benchmark' +include { SV_GERMLINE_BENCHMARK } from '../subworkflows/local/sv_germline_benchmark' include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' -include { VCF_CONVERSIONS } from '../subworkflows/local/vcf_conversion' +include { SV_VCF_CONVERSIONS } from '../subworkflows/local/sv_vcf_conversion' include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TEST } from '../subworkflows/local/report_vcf_statistics' include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_vcf_statistics' @@ -94,32 +94,49 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) ch_input = INPUT_CHECK.out.ch_sample + // TODO: SMALL VARIANT BENCHMARKING + ch_input.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{input} + + out_vcf_ch = Channel.empty() + + // PREPROCESSES + // - // SUBWORKFLOW: VCF_CONVERSIONS + // SUBWORKFLOW: SV_VCF_CONVERSIONS // - // Standardize VCFs, tool spesific modifications - VCF_CONVERSIONS( - ch_input, + // Standardize SV VCFs, tool spesific modifications + SV_VCF_CONVERSIONS( + input.sv, ref ) - ch_versions = ch_versions.mix(VCF_CONVERSIONS.out.versions) + ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) + out_vcf_ch = out_vcf_ch.mix(SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])}) + out_vcf_ch = out_vcf_ch.mix(input.small) + out_vcf_ch = out_vcf_ch.mix(input.cnv) // // SUBWORKFLOW: Prepare and normalize input vcfs // + PREPARE_VCFS_TEST( + out_vcf_ch, + ref + ) + ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) + PREPARE_VCFS_TRUTH( truth, ref ) ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) - PREPARE_VCFS_TEST( - VCF_CONVERSIONS.out.vcf_ch, - ref - ) - ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) + // VCF REPORTS AND STATS // // SUBWORKFLOW: GET STATISTICS OF FILES @@ -145,15 +162,24 @@ workflow VARIANTBENCHMARKING { .set{bench_ch} } + // BENCHMARKS + + bench_ch.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{input} + // - // SUBWORKFLOW: GERMLINE_BENCHMARK + // SUBWORKFLOW: SV_GERMLINE_BENCHMARK // //Benchmarking spesific to germline samples - GERMLINE_BENCHMARK( - bench_ch, + SV_GERMLINE_BENCHMARK( + input.sv, ref ) - ch_versions = ch_versions.mix(GERMLINE_BENCHMARK.out.versions) + ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) // TODO: SOMATIC EBNCHMARKING if (params.analysis.contains("somatic")){ @@ -170,6 +196,7 @@ workflow VARIANTBENCHMARKING { // TODO: BENCHMARKING OF CNV + // https://bioconductor.org/packages/release/bioc/manuals/CNVfilteR/man/CNVfilteR.pdf // TODO: TRIO ANALYSIS : MENDELIAN INCONSISTANCE From 1220b493db59cc4eee63a977f063cc351d29a9b7 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Thu, 22 Feb 2024 12:14:56 +0100 Subject: [PATCH 018/292] pin nf-validation version --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 1f4f8d7..3cdeebe 100644 --- a/nextflow.config +++ b/nextflow.config @@ -216,7 +216,7 @@ singularity.registry = '' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } From 8115a869cb8f72e290bc784f66f4fbc3ed09fbb0 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 22 Feb 2024 13:12:50 +0100 Subject: [PATCH 019/292] lint --- assets/multiqc_config.yml | 2 +- conf/test.config | 8 ++-- modules.json | 10 ---- nextflow.config | 7 +-- nextflow_schema.json | 99 ++++++++++++++++++++++++++++++--------- 5 files changed, 84 insertions(+), 42 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 9653868..d26a71b 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the nf-core/variantbenchmarking + This report has been generated by the nf-core/variantbenchmarking analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: diff --git a/conf/test.config b/conf/test.config index b2c3ec8..daf0cc0 100644 --- a/conf/test.config +++ b/conf/test.config @@ -28,10 +28,10 @@ params { // Processes analysis = 'germline' - method = 'truvari,svanalyzer,wittyer' // --not working for now : vcfdist - similarity = 0 // determines the sequence similarity level in benchmarking. - preprocess = "normalization, deduplication" - min_sv_size = 50 + method = 'truvari,svanalyzer' // --not working for now : vcfdist + //similarity = 0 // determines the sequence similarity level in benchmarking. + //preprocess = "normalization, deduplication" + //min_sv_size = 50 truth = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" } diff --git a/modules.json b/modules.json index e608e79..2ee978b 100644 --- a/modules.json +++ b/modules.json @@ -25,16 +25,6 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "happy/happy": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "happy/sompy": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, "multiqc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", diff --git a/nextflow.config b/nextflow.config index dff170d..d862b0e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,7 +12,7 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options input = null - output = "results" + outdir = "results" // Preprocess spesific parameters // normalization includes braking down multiallelic samples -m any @@ -44,11 +44,6 @@ params { igenomes_ignore = false analysis = "germline" - truth_config_ignore = false - sv_bed = null - snv_bed = null - sdf_file = null - // MultiQC options multiqc_config = null multiqc_title = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 9cb22d3..ecb9839 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -13,33 +13,81 @@ "required": ["input", "outdir"], "properties": { "input": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/csv", - "schema": "assets/schema_input.json", - "pattern": "^\\S+\\.(csv|tsv|yaml)$", - "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/variantbenchmarking/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/csv", + "schema": "assets/schema_input.json", + "pattern": "^\\S+\\.(csv|tsv|yaml)$", + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/variantbenchmarking/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" }, "outdir": { - "type": "string", - "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "analysis": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "preprocess": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "standardization": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "method": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "min_sv_size": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "dup_to_ins": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "bnd_to_inv": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "gridss_annotate": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" }, "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" }, "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" } } }, @@ -65,6 +113,15 @@ "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", "fa_icon": "far fa-file-code" }, + "fai": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "description": "Path to FAI genome file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, "igenomes_ignore": { "type": "boolean", "description": "Do not load the iGenomes reference config.", From 5074a49dab8c9176cec7e1cc7502e78ae7e13bd8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 22 Feb 2024 13:14:46 +0100 Subject: [PATCH 020/292] params --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index daf0cc0..a9ca4ca 100644 --- a/conf/test.config +++ b/conf/test.config @@ -31,7 +31,7 @@ params { method = 'truvari,svanalyzer' // --not working for now : vcfdist //similarity = 0 // determines the sequence similarity level in benchmarking. //preprocess = "normalization, deduplication" - //min_sv_size = 50 + min_sv_size = 0 truth = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" } From f2a922879565e6163ea5984be36e6b2c5c9a3ca5 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 22 Feb 2024 13:23:16 +0100 Subject: [PATCH 021/292] similarity --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index a9ca4ca..f9b9c50 100644 --- a/conf/test.config +++ b/conf/test.config @@ -29,7 +29,7 @@ params { // Processes analysis = 'germline' method = 'truvari,svanalyzer' // --not working for now : vcfdist - //similarity = 0 // determines the sequence similarity level in benchmarking. + similarity = 0 // determines the sequence similarity level in benchmarking. //preprocess = "normalization, deduplication" min_sv_size = 0 From eb514e863f24d43d0ecbdb0ba294fc63afa62c0e Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 22 Feb 2024 13:45:16 +0100 Subject: [PATCH 022/292] similarity --- conf/test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test.config b/conf/test.config index f9b9c50..5500d37 100644 --- a/conf/test.config +++ b/conf/test.config @@ -30,7 +30,7 @@ params { analysis = 'germline' method = 'truvari,svanalyzer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. - //preprocess = "normalization, deduplication" + //preprocess = "normalization, deduplication" min_sv_size = 0 truth = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" From c2c91c3f848d679740ae741e77140cdb16a2bc57 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Thu, 22 Feb 2024 14:16:17 +0100 Subject: [PATCH 023/292] run prettier --- assets/schema_input.json | 53 ++- assets/svync/delly.yaml | 1 - assets/svync/gridss.yaml | 1 - assets/svync/manta.yaml | 1 - assets/svync/smoove.yaml | 1 - modules.json | 108 +++---- nextflow_schema.json | 674 +++++++++++++++++++-------------------- 7 files changed, 417 insertions(+), 422 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index ee44386..af65924 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,29 +1,28 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/assets/schema_input.json", - "title": "nf-core/variantbenchmarking pipeline - params.input schema", - "description": "Schema for the file provided with params.input", - "type": "array", - "items": { - "type": "object", - "properties": { - "test_vcf": { - "type": "string", - "pattern": "", - "errorMessage": "Test VCF must be provided, cannot contain spaces and must have extension '.vcf.gz'" - }, - "caller": { - "type": "string", - "pattern": "^\\S+$", - "errorMessage": "Name of the variant caller used to generate test file" - }, - "vartype": { - "type": "string", - "pattern": "^\\S+$", - "errorMessage": "Variant type to benchmark" - } - - }, - "required": ["test_vcf","caller","vartype"] - } + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/assets/schema_input.json", + "title": "nf-core/variantbenchmarking pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "test_vcf": { + "type": "string", + "pattern": "", + "errorMessage": "Test VCF must be provided, cannot contain spaces and must have extension '.vcf.gz'" + }, + "caller": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Name of the variant caller used to generate test file" + }, + "vartype": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Variant type to benchmark" + } + }, + "required": ["test_vcf", "caller", "vartype"] + } } diff --git a/assets/svync/delly.yaml b/assets/svync/delly.yaml index f820a1d..5672ba8 100644 --- a/assets/svync/delly.yaml +++ b/assets/svync/delly.yaml @@ -66,4 +66,3 @@ format: number: 2 type: integer description: Split-read support for the ref and alt alleles in the order listed - diff --git a/assets/svync/gridss.yaml b/assets/svync/gridss.yaml index 62c8c8a..f29f944 100644 --- a/assets/svync/gridss.yaml +++ b/assets/svync/gridss.yaml @@ -62,4 +62,3 @@ format: number: 2 type: integer description: Split-read support for the ref and alt alleles in the order listed - diff --git a/assets/svync/manta.yaml b/assets/svync/manta.yaml index eaa5869..51a542b 100644 --- a/assets/svync/manta.yaml +++ b/assets/svync/manta.yaml @@ -63,4 +63,3 @@ format: number: 2 type: integer description: Split-read support for the ref and alt alleles in the order listed - diff --git a/assets/svync/smoove.yaml b/assets/svync/smoove.yaml index ee277a7..f214cdf 100644 --- a/assets/svync/smoove.yaml +++ b/assets/svync/smoove.yaml @@ -58,4 +58,3 @@ format: number: 2 type: integer description: Split-read support for the ref and alt alleles in the order listed - diff --git a/modules.json b/modules.json index 91ae3d9..f0f4e73 100644 --- a/modules.json +++ b/modules.json @@ -4,63 +4,63 @@ "repos": { "https://github.com/nf-core/modules.git": { "modules": { - "nf-core": { - "bcftools/annotate": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "bcftools/norm": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "fastqc": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "multiqc": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "tabix/tabix": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "truvari/bench": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "survivor/stats": { - "branch": "master", - "git_sha": "00d03755abd2cac54a039525716aeabea9efb9e7", - "installed_by": ["modules"] + "nf-core": { + "bcftools/annotate": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "bcftools/norm": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "fastqc": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "multiqc": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "tabix/tabix": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "truvari/bench": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "survivor/stats": { + "branch": "master", + "git_sha": "00d03755abd2cac54a039525716aeabea9efb9e7", + "installed_by": ["modules"] + } } - } }, "subworkflows": { - "nf-core": { - "utils_nextflow_pipeline": { - "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", - "installed_by": ["subworkflows"] - }, - "utils_nfcore_pipeline": { - "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", - "installed_by": ["subworkflows"] - }, - "utils_nfvalidation_plugin": { - "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", - "installed_by": ["subworkflows"] - } - } - } + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } + } } } } diff --git a/nextflow_schema.json b/nextflow_schema.json index 906c546..34e32b6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,346 +1,346 @@ { - "$schema": "http://json-schema.org/draft-07/schema", - "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", - "title": "nf-core/variantbenchmarking pipeline parameters", - "description": "Master benchmarking pipeline for variant callers ", - "type": "object", - "definitions": { - "input_output_options": { - "title": "Input/output options", - "type": "object", - "fa_icon": "fas fa-terminal", - "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], - "properties": { - "input": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/csv", - "schema": "assets/schema_input.json", - "pattern": "^\\S+\\.(csv|tsv|yaml|yml|json)$", - "description": "Path to comma-separated file containing information about the samples in the experiment.", - "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/variantbenchmarking/usage#samplesheet-input).", - "fa_icon": "fas fa-file-csv" - }, - "outdir": { - "type": "string", - "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", - "fa_icon": "fas fa-folder-open" - }, - "analysis": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "preprocess": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "standardization": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "method": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "min_sv_size": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "dup_to_ins": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "bnd_to_inv": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "gridss_annotate": { - "type": "string", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "email": { - "type": "string", - "description": "Email address for completion summary.", - "fa_icon": "fas fa-envelope", - "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" - }, - "multiqc_title": { - "type": "string", - "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", - "fa_icon": "fas fa-file-signature" - } - } - }, - "reference_genome_options": { - "title": "Reference genome options", - "type": "object", - "fa_icon": "fas fa-dna", - "description": "Reference genome related files and options required for the workflow.", - "properties": { - "genome": { - "type": "string", - "description": "Name of iGenomes reference.", - "fa_icon": "fas fa-book", - "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." - }, - "fasta": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/plain", - "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", - "description": "Path to FASTA genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code" - }, - "fai": { - "type": "string", - "format": "file-path", - "exists": true, - "mimetype": "text/plain", - "description": "Path to FAI genome file.", - "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", - "fa_icon": "far fa-file-code" - }, - "igenomes_ignore": { - "type": "boolean", - "description": "Do not load the iGenomes reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." - } - } - }, - "institutional_config_options": { - "title": "Institutional config options", - "type": "object", - "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", - "properties": { - "custom_config_version": { - "type": "string", - "description": "Git commit id for Institutional configs.", - "default": "master", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "custom_config_base": { - "type": "string", - "description": "Base directory for Institutional configs.", - "default": "https://raw.githubusercontent.com/nf-core/configs/master", - "hidden": true, - "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", - "fa_icon": "fas fa-users-cog" - }, - "config_profile_name": { - "type": "string", - "description": "Institutional config name.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_description": { - "type": "string", - "description": "Institutional config description.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_contact": { - "type": "string", - "description": "Institutional config contact information.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - }, - "config_profile_url": { - "type": "string", - "description": "Institutional config URL link.", - "hidden": true, - "fa_icon": "fas fa-users-cog" - } - } - }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", + "title": "nf-core/variantbenchmarking pipeline parameters", + "description": "Master benchmarking pipeline for variant callers ", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["input", "outdir"], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/csv", + "schema": "assets/schema_input.json", + "pattern": "^\\S+\\.(csv|tsv|yaml|yml|json)$", + "description": "Path to comma-separated file containing information about the samples in the experiment.", + "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/variantbenchmarking/usage#samplesheet-input).", + "fa_icon": "fas fa-file-csv" + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "analysis": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "preprocess": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "standardization": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "method": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "min_sv_size": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "dup_to_ins": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "bnd_to_inv": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "gridss_annotate": { + "type": "string", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, + "email": { + "type": "string", + "description": "Email address for completion summary.", + "fa_icon": "fas fa-envelope", + "help_text": "Set this parameter to your e-mail address to get a summary e-mail with details of the run sent to you when the workflow exits. If set in your user config file (`~/.nextflow/config`) then you don't need to specify this on the command line for every run.", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$" + }, + "multiqc_title": { + "type": "string", + "description": "MultiQC report title. Printed as page header, used for filename if not otherwise specified.", + "fa_icon": "fas fa-file-signature" + } + } + }, + "reference_genome_options": { + "title": "Reference genome options", + "type": "object", + "fa_icon": "fas fa-dna", + "description": "Reference genome related files and options required for the workflow.", + "properties": { + "genome": { + "type": "string", + "description": "Name of iGenomes reference.", + "fa_icon": "fas fa-book", + "help_text": "If using a reference genome configured in the pipeline using iGenomes, use this parameter to give the ID for the reference. This is then used to build the full paths for all required reference genome files e.g. `--genome GRCh38`. \n\nSee the [nf-core website docs](https://nf-co.re/usage/reference_genomes) for more details." + }, + "fasta": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", + "description": "Path to FASTA genome file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, + "fai": { + "type": "string", + "format": "file-path", + "exists": true, + "mimetype": "text/plain", + "description": "Path to FAI genome file.", + "help_text": "This parameter is *mandatory* if `--genome` is not specified. If you don't have a BWA index available this will be generated for you automatically. Combine with `--save_reference` to save BWA index for future runs.", + "fa_icon": "far fa-file-code" + }, + "igenomes_ignore": { + "type": "boolean", + "description": "Do not load the iGenomes reference config.", + "fa_icon": "fas fa-ban", + "hidden": true, + "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + } + } + }, + "institutional_config_options": { + "title": "Institutional config options", + "type": "object", + "fa_icon": "fas fa-university", + "description": "Parameters used to describe centralised config profiles. These should not be edited.", + "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "properties": { + "custom_config_version": { + "type": "string", + "description": "Git commit id for Institutional configs.", + "default": "master", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "custom_config_base": { + "type": "string", + "description": "Base directory for Institutional configs.", + "default": "https://raw.githubusercontent.com/nf-core/configs/master", + "hidden": true, + "help_text": "If you're running offline, Nextflow will not be able to fetch the institutional config files from the internet. If you don't need them, then this is not a problem. If you do need them, you should download the files from the repo and tell Nextflow where to find them with this parameter.", + "fa_icon": "fas fa-users-cog" + }, + "config_profile_name": { + "type": "string", + "description": "Institutional config name.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_description": { + "type": "string", + "description": "Institutional config description.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_contact": { + "type": "string", + "description": "Institutional config contact information.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + }, + "config_profile_url": { + "type": "string", + "description": "Institutional config URL link.", + "hidden": true, + "fa_icon": "fas fa-users-cog" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 16, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "128.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "240.h", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "email_on_fail": { + "type": "string", + "description": "Email address for completion summary, only when pipeline fails.", + "fa_icon": "fas fa-exclamation-triangle", + "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", + "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", + "hidden": true + }, + "plaintext_email": { + "type": "boolean", + "description": "Send plain-text email instead of HTML.", + "fa_icon": "fas fa-remove-format", + "hidden": true + }, + "max_multiqc_email_size": { + "type": "string", + "description": "File size limit when attaching MultiQC reports to summary emails.", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "default": "25.MB", + "fa_icon": "fas fa-file-upload", + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Do not use coloured log outputs.", + "fa_icon": "fas fa-palette", + "hidden": true + }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", + "hidden": true + }, + "multiqc_config": { + "type": "string", + "format": "file-path", + "description": "Custom config file to supply to MultiQC.", + "fa_icon": "fas fa-cog", + "hidden": true + }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, + "validate_params": { + "type": "boolean", + "description": "Boolean whether to validate parameters against the schema at runtime", + "default": true, + "fa_icon": "fas fa-check-square", + "hidden": true + }, + "validationShowHiddenParams": { + "type": "boolean", + "fa_icon": "far fa-eye-slash", + "description": "Show all params when using `--help`", + "hidden": true, + "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "validationFailUnrecognisedParams": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters fails when an unrecognised parameter is found.", + "hidden": true, + "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." + }, + "validationLenientMode": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters in lenient more.", + "hidden": true, + "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + } + } } - } }, - "generic_options": { - "title": "Generic options", - "type": "object", - "fa_icon": "fas fa-file-import", - "description": "Less common options for the pipeline, typically set in a config file.", - "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", - "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "version": { - "type": "boolean", - "description": "Display version and exit.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, - "publish_dir_mode": { - "type": "string", - "default": "copy", - "description": "Method used to save pipeline results to output directory.", - "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", - "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], - "hidden": true - }, - "email_on_fail": { - "type": "string", - "description": "Email address for completion summary, only when pipeline fails.", - "fa_icon": "fas fa-exclamation-triangle", - "pattern": "^([a-zA-Z0-9_\\-\\.]+)@([a-zA-Z0-9_\\-\\.]+)\\.([a-zA-Z]{2,5})$", - "help_text": "An email address to send a summary email to when the pipeline is completed - ONLY sent if the pipeline does not exit successfully.", - "hidden": true - }, - "plaintext_email": { - "type": "boolean", - "description": "Send plain-text email instead of HTML.", - "fa_icon": "fas fa-remove-format", - "hidden": true + "allOf": [ + { + "$ref": "#/definitions/input_output_options" }, - "max_multiqc_email_size": { - "type": "string", - "description": "File size limit when attaching MultiQC reports to summary emails.", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "default": "25.MB", - "fa_icon": "fas fa-file-upload", - "hidden": true + { + "$ref": "#/definitions/reference_genome_options" }, - "monochrome_logs": { - "type": "boolean", - "description": "Do not use coloured log outputs.", - "fa_icon": "fas fa-palette", - "hidden": true + { + "$ref": "#/definitions/institutional_config_options" }, - "hook_url": { - "type": "string", - "description": "Incoming hook URL for messaging service", - "fa_icon": "fas fa-people-group", - "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", - "hidden": true + { + "$ref": "#/definitions/max_job_request_options" }, - "multiqc_config": { - "type": "string", - "format": "file-path", - "description": "Custom config file to supply to MultiQC.", - "fa_icon": "fas fa-cog", - "hidden": true - }, - "multiqc_logo": { - "type": "string", - "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", - "fa_icon": "fas fa-image", - "hidden": true - }, - "multiqc_methods_description": { - "type": "string", - "description": "Custom MultiQC yaml file containing HTML including a methods description.", - "fa_icon": "fas fa-cog" - }, - "validate_params": { - "type": "boolean", - "description": "Boolean whether to validate parameters against the schema at runtime", - "default": true, - "fa_icon": "fas fa-check-square", - "hidden": true - }, - "validationShowHiddenParams": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "validationFailUnrecognisedParams": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters fails when an unrecognised parameter is found.", - "hidden": true, - "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." - }, - "validationLenientMode": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters in lenient more.", - "hidden": true, - "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + { + "$ref": "#/definitions/generic_options" } - } - } - }, - "allOf": [ - { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/reference_genome_options" - }, - { - "$ref": "#/definitions/institutional_config_options" - }, - { - "$ref": "#/definitions/max_job_request_options" - }, - { - "$ref": "#/definitions/generic_options" - } - ] + ] } From 8e8f9fe96d0efbd3c89449b00688b0cb2664f6b1 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Thu, 22 Feb 2024 14:21:56 +0100 Subject: [PATCH 024/292] make eclint happy --- bin/add_svtype.py | 4 ++-- bin/annotate_repeat_regions.py | 8 ++++---- bin/id_annotation.py | 2 +- bin/simple_event_annotator.R | 12 ++++++------ modules/local/awk_sort.nf | 2 +- modules/local/truvari_refine.nf | 6 +++--- subworkflows/local/sv_vcf_conversion.nf | 4 ++-- subworkflows/local/visualizations.nf | 4 ++-- 8 files changed, 21 insertions(+), 21 deletions(-) diff --git a/bin/add_svtype.py b/bin/add_svtype.py index d93981e..e2b5595 100644 --- a/bin/add_svtype.py +++ b/bin/add_svtype.py @@ -37,7 +37,7 @@ for v in in_vcf: svlen=(len(v.alts[0])-len(v.ref)) limit=args.svlength - + if (svlen < -1*limit): v.info.update({'SVTYPE':"DEL"}) v.info.update({'SVLEN':svlen}) @@ -50,7 +50,7 @@ counter=counter+1 else: anno_vcf.write(v) - + in_vcf.close() anno_vcf.close() logger.info('Finished adding SVTYE/SVLEN to VCF: {}'.format(counter)) diff --git a/bin/annotate_repeat_regions.py b/bin/annotate_repeat_regions.py index c92bcaa..1002660 100644 --- a/bin/annotate_repeat_regions.py +++ b/bin/annotate_repeat_regions.py @@ -31,7 +31,7 @@ def print_AF(in_vcf,samplerep,repeatmask,segdup): simple_repeat=temp1.iloc[0].period else: simple_repeat='None' - + temp2=repeatmask[(repeatmask['genoName']== v.chrom) &( (( v.start > repeatmask['genoStart']-1 ) & ( v.start < repeatmask['genoEnd'] +1 ) )| (( v.stop > repeatmask['genoStart'] -1) & ( v.stop < repeatmask['genoEnd'] +1 ))) ] if len(temp2) > 0: @@ -40,7 +40,7 @@ def print_AF(in_vcf,samplerep,repeatmask,segdup): else: repname='None' repclass='None' - + temp3=segdup[(segdup['chrom']== v.chrom) &( (( v.start > segdup['chromStart']-1 ) & ( v.start < segdup['chromEnd'] +1 ) )| (( v.stop > segdup['chromStart'] -1) & ( v.stop < segdup['chromEnd'] +1 ))) ] if len(temp3) > 0: segdups=temp3.iloc[0].fracMatch @@ -53,13 +53,13 @@ def print_AF(in_vcf,samplerep,repeatmask,segdup): print('DEL', v.ref, len(v.alts[0])- len(v.ref) , v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) else: print('DEL', '...', len(v.alts[0])- len(v.ref) , v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) - + elif len(v.alts[0]) - len(v.ref) > 49: if len(v.ref) - len(v.alts[0]) < 1001: print('INS', v.alts[0],len(v.alts[0]) - len(v.ref), v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) else: print('INS', '...',len(v.alts[0]) - len(v.ref), v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) - + else: continue diff --git a/bin/id_annotation.py b/bin/id_annotation.py index 1289459..a41e653 100644 --- a/bin/id_annotation.py +++ b/bin/id_annotation.py @@ -41,7 +41,7 @@ string=str(v.chrom) +'_'+ str(v.pos) + '_SNP' v.id =string - + in_vcf.close() anno_vcf.close() logger.info('Finished') diff --git a/bin/simple_event_annotator.R b/bin/simple_event_annotator.R index ec99571..1325014 100644 --- a/bin/simple_event_annotator.R +++ b/bin/simple_event_annotator.R @@ -17,11 +17,11 @@ library(getopt) simpleEventType <- function(gr) { - return(ifelse(seqnames(gr) != seqnames(partner(gr)), "ITX", # inter-chromosomosal - ifelse(gr$insLen >= abs(gr$svLen) * 0.7, "INS", # TODO: improve classification of complex events - ifelse(strand(gr) == strand(partner(gr)), "INV", - ifelse(xor(start(gr) < start(partner(gr)), strand(gr) == "-"), "DEL", - "DUP"))))) + return(ifelse(seqnames(gr) != seqnames(partner(gr)), "ITX", # inter-chromosomosal + ifelse(gr$insLen >= abs(gr$svLen) * 0.7, "INS", # TODO: improve classification of complex events + ifelse(strand(gr) == strand(partner(gr)), "INV", + ifelse(xor(start(gr) < start(partner(gr)), strand(gr) == "-"), "DEL", + "DUP"))))) } cmdArgs = commandArgs(TRUE) print(cmdArgs) @@ -56,4 +56,4 @@ writeVcf(vcf, outfile) # ) ## Just the lower of the two breakends so we don't output everything twice #simplebed <- simplebed[simplebed$start < simplebed$end,] -#write.table(simplebed, "chr12.1527326.DEL1024.simple.bed", quote=FALSE, sep='\t', row.names=FALSE, col.names=FALSE) \ No newline at end of file +#write.table(simplebed, "chr12.1527326.DEL1024.simple.bed", quote=FALSE, sep='\t', row.names=FALSE, col.names=FALSE) diff --git a/modules/local/awk_sort.nf b/modules/local/awk_sort.nf index cb2dc29..c115593 100644 --- a/modules/local/awk_sort.nf +++ b/modules/local/awk_sort.nf @@ -24,7 +24,7 @@ process AWK_SORT { if (vcf.getExtension() == "gz"){ """ - bgzip -d $vcf + bgzip -d $vcf cat $zipname | awk '\$1 ~ /^#/ {print \$0;next} {print \$0 | "sort -k1,1 -k2,2n"}' > ${zipname}.tmp.vcf bgzip -c ${zipname}.tmp.vcf > ${zipname}.tmp.vcf.gz tabix -p vcf ${zipname}.tmp.vcf.gz diff --git a/modules/local/truvari_refine.nf b/modules/local/truvari_refine.nf index e10d618..517480d 100644 --- a/modules/local/truvari_refine.nf +++ b/modules/local/truvari_refine.nf @@ -25,10 +25,10 @@ process TRUVARI_REFINE { """ truvari refine \\ --use-original-vcfs \\ - --reference $fasta \\ - --regions $bed \\ + --reference $fasta \\ + --regions $bed \\ $bench - . + . cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 18e5ae9..153f477 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -77,7 +77,7 @@ workflow SV_VCF_CONVERSIONS { out_vcf_ch = out_vcf_ch.mix(input.other) vcf_ch = out_vcf_ch - // https://github.com/srbehera/DRAGEN_Analysis/blob/main/convertInversion.py + // https://github.com/srbehera/DRAGEN_Analysis/blob/main/convertInversion.py } @@ -104,7 +104,7 @@ workflow SV_VCF_CONVERSIONS { out_vcf_ch = out_vcf_ch.mix(input.other) vcf_ch = out_vcf_ch } - // https://github.com/EUCANCan/variant-extractor/blob/main/examples/vcf_to_csv.py + // https://github.com/EUCANCan/variant-extractor/blob/main/examples/vcf_to_csv.py emit: vcf_ch diff --git a/subworkflows/local/visualizations.nf b/subworkflows/local/visualizations.nf index 29f727c..d72d3d6 100644 --- a/subworkflows/local/visualizations.nf +++ b/subworkflows/local/visualizations.nf @@ -26,8 +26,8 @@ workflow PREPARE_VCFS_TRUTH { // Check tool spesific conversions - // https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R#L9 - // GRIDSS simple event annotation + // https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R#L9 + // GRIDSS simple event annotation emit: vcf_ch From 87797f36097b709a6b133d5d758174c7d85edc83 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Thu, 22 Feb 2024 15:03:10 +0100 Subject: [PATCH 025/292] some more fixes to get the pipeline running again --- assets/schema_input.json | 9 ++-- conf/test.config | 3 ++ main.nf | 37 ++++++++++++---- nextflow_schema.json | 1 + .../main.nf | 16 ------- workflows/variantbenchmarking.nf | 42 +++++++++++-------- 6 files changed, 63 insertions(+), 45 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index af65924..0cde408 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -9,18 +9,21 @@ "properties": { "test_vcf": { "type": "string", - "pattern": "", + "pattern": "\\S+\\.vcf(\\.gz)?$", + "format": "file-path", "errorMessage": "Test VCF must be provided, cannot contain spaces and must have extension '.vcf.gz'" }, "caller": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Name of the variant caller used to generate test file" + "errorMessage": "Name of the variant caller used to generate test file", + "meta": ["caller"] }, "vartype": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Variant type to benchmark" + "errorMessage": "Variant type to benchmark", + "meta": ["vartype"] } }, "required": ["test_vcf", "caller", "vartype"] diff --git a/conf/test.config b/conf/test.config index 5500d37..82e0a78 100644 --- a/conf/test.config +++ b/conf/test.config @@ -22,9 +22,12 @@ params { // Input data sample = "HG002" input = 'assets/samplesheet.csv' + fasta = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta' + fai = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai' // Genome references genome = 'hg38' + igenomes_ignore = false // Processes analysis = 'germline' diff --git a/main.nf b/main.nf index a474dc7..dfd98c5 100644 --- a/main.nf +++ b/main.nf @@ -11,6 +11,16 @@ nextflow.enable.dsl = 2 +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + GENOME PARAMETER VALUES +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' + +params.fasta = getGenomeAttribute('fasta') +params.fai = getGenomeAttribute('fai') + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS @@ -21,19 +31,30 @@ include { VARIANTBENCHMARKING } from './workflows/variantbenchmarking' include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' -include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + NAMED WORKFLOW FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = getGenomeAttribute('fasta') -params.fai = getGenomeAttribute('fai') +// WORKFLOW: Run main nf-core/sarek analysis pipeline +workflow NFCORE_VARIANTBENCHMARKING { + + take: + ch_samplesheet + + main: + + // + // WORKFLOW: Run pipeline + // + VARIANTBENCHMARKING( + ch_samplesheet + ) + + emit: + multiqc_report = VARIANTBENCHMARKING.out.multiqc_report // channel: /path/to/multiqc_report.html +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ diff --git a/nextflow_schema.json b/nextflow_schema.json index 34e32b6..25fb870 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -96,6 +96,7 @@ "type": "object", "fa_icon": "fas fa-dna", "description": "Reference genome related files and options required for the workflow.", + "required": ["fasta", "fai"], "properties": { "genome": { "type": "string", diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index dc90ad9..d04f860 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -82,22 +82,6 @@ workflow PIPELINE_INITIALISATION { // Channel .fromSamplesheet("input") - .map { - meta, fastq_1, fastq_2 -> - if (!fastq_2) { - return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] - } else { - return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] - } - } - .groupTuple() - .map { - validateInputSamplesheet(it) - } - .map { - meta, fastqs -> - return [ meta, fastqs.flatten() ] - } .set { ch_samplesheet } emit: diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index ac1318f..095e119 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -1,20 +1,3 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowVariantbenchmarking.initialise(params, log) - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ CONFIG FILES @@ -40,6 +23,13 @@ include { paramsSummaryMap } from 'plugin/nf-validation' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' +include { SOMATIC_BENCHMARK } from '../subworkflows/local/somatic_benchmark' +include { SV_GERMLINE_BENCHMARK } from '../subworkflows/local/sv_germline_benchmark' +include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' +include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' +include { SV_VCF_CONVERSIONS } from '../subworkflows/local/sv_vcf_conversion' +include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TEST } from '../subworkflows/local/report_vcf_statistics' +include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_vcf_statistics' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -54,7 +44,23 @@ workflow VARIANTBENCHMARKING { main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() + + // check mandatory parameters + println(params.fasta) + println(params.fai) + ref = Channel.fromPath([params.fasta,params.fai], checkIfExists: true).collect() + + // check high confidence files + + truth = params.truth ? Channel.fromPath(params.truth, checkIfExists: true).collect() + : Channel.empty() + + high_conf = params.high_conf ? Channel.fromPath(params.high_conf, checkIfExists: true).collect() + : Channel.empty() + + // TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID ch_samplesheet.branch{ sv: it[0].vartype == "sv" From 53e43a6a763907921025c44d026dda57aaa0005d Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Thu, 22 Feb 2024 15:18:20 +0100 Subject: [PATCH 026/292] fix last issues --- assets/schema_input.json | 2 +- conf/test.config | 3 --- nextflow.config | 2 ++ 3 files changed, 3 insertions(+), 4 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 0cde408..493d1d9 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -17,7 +17,7 @@ "type": "string", "pattern": "^\\S+$", "errorMessage": "Name of the variant caller used to generate test file", - "meta": ["caller"] + "meta": ["id"] }, "vartype": { "type": "string", diff --git a/conf/test.config b/conf/test.config index 82e0a78..5500d37 100644 --- a/conf/test.config +++ b/conf/test.config @@ -22,12 +22,9 @@ params { // Input data sample = "HG002" input = 'assets/samplesheet.csv' - fasta = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta' - fai = 'https://github.com/nf-core/test-datasets/raw/modules/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai' // Genome references genome = 'hg38' - igenomes_ignore = false // Processes analysis = 'germline' diff --git a/nextflow.config b/nextflow.config index 56ee614..169628c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -19,6 +19,8 @@ params { // deduplication removes one of the variants in the same position preprocess = "" standardization = "" + harmonize = false + high_conf = "" // Benchmarking method method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist From 91993d7bbad8d7d1714ef008e59771dc96f6106f Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Thu, 22 Feb 2024 15:43:41 +0100 Subject: [PATCH 027/292] update modules.json --- modules.json | 98 +++++++++++++++++++++++++++++++++++++++++++++++++--- 1 file changed, 94 insertions(+), 4 deletions(-) diff --git a/modules.json b/modules.json index f0f4e73..cd71108 100644 --- a/modules.json +++ b/modules.json @@ -10,27 +10,87 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, + "bcftools/isec": { + "branch": "master", + "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", + "installed_by": ["modules"] + }, "bcftools/norm": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "fastqc": { + "bcftools/reheader": { + "branch": "master", + "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", + "installed_by": ["modules"] + }, + "bcftools/sort": { + "branch": "master", + "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", + "installed_by": ["modules"] + }, + "bcftools/stats": { + "branch": "master", + "git_sha": "41dfa13929d2c178855159a69d2e2958c52be155", + "installed_by": ["modules"] + }, + "bcftools/view": { + "branch": "master", + "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", + "installed_by": ["modules"] + }, + "bedgovcf": { + "branch": "master", + "git_sha": "338a5572efbda56110cea2f657615920d8dce58c", + "installed_by": ["modules"] + }, + "bedtools/coverage": { + "branch": "master", + "git_sha": "1d1cb7bfef6cf67fbc7faafa6992ad8bdc3045b3", + "installed_by": ["modules"] + }, + "bedtools/genomecov": { + "branch": "master", + "git_sha": "90aef30f432332bdf0ce9f4b9004aa5d5c4960bb", + "installed_by": ["modules"] + }, + "bedtools/intersect": { + "branch": "master", + "git_sha": "90aef30f432332bdf0ce9f4b9004aa5d5c4960bb", + "installed_by": ["modules"] + }, + "bedtools/merge": { + "branch": "master", + "git_sha": "90aef30f432332bdf0ce9f4b9004aa5d5c4960bb", + "installed_by": ["modules"] + }, + "bedtools/subtract": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "multiqc": { + "bedtools/unionbedg": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "tabix/tabix": { + "fastqc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, - "truvari/bench": { + "manta/convertinversion": { + "branch": "master", + "git_sha": "b8ff8e32e305587ac2c7909db3058fbbba2a859a", + "installed_by": ["modules"] + }, + "multiqc": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "survivor/filter": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] @@ -39,6 +99,36 @@ "branch": "master", "git_sha": "00d03755abd2cac54a039525716aeabea9efb9e7", "installed_by": ["modules"] + }, + "svanalyzer/svbenchmark": { + "branch": "master", + "git_sha": "419065b3a66b857af8b49d41dc1ba3858821da22", + "installed_by": ["modules"] + }, + "svync": { + "branch": "master", + "git_sha": "49925b34411ab0aef3fdb61a4d516ef36eceec05", + "installed_by": ["modules"] + }, + "tabix/bgzip": { + "branch": "master", + "git_sha": "31c0b49f6527ef196e89eca49a36af2de71711f8", + "installed_by": ["modules"] + }, + "tabix/bgziptabix": { + "branch": "master", + "git_sha": "5e7b1ef9a5a2d9258635bcbf70fcf37dacd1b247", + "installed_by": ["modules"] + }, + "tabix/tabix": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "truvari/bench": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] } } }, From 7b4327a4aac3c0b9ba973469cd7c5aac4affb164 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 23 Feb 2024 16:19:32 +0100 Subject: [PATCH 028/292] svync input files retained --- assets/samplesheet_HG002.csv | 5 ----- assets/samplesheet_HG002_hg37.csv | 5 +++++ assets/samplesheet_HG002_hg38.csv | 9 +++----- assets/samplesheet_SEQC2.csv | 4 ++-- assets/svync/default.yaml | 0 conf/modules.config | 2 +- conf/test.config | 7 +++--- modules/nf-core/multiqc/main.nf | 2 +- nextflow_schema.json | 4 ++-- .../execution_trace_2024-02-23_11-23-40.txt | 1 + .../execution_trace_2024-02-23_11-25-34.txt | 1 + subworkflows/local/sv_vcf_conversion.nf | 22 +++++++++++-------- workflows/variantbenchmarking.nf | 8 ++++--- 13 files changed, 38 insertions(+), 32 deletions(-) delete mode 100644 assets/samplesheet_HG002.csv create mode 100644 assets/samplesheet_HG002_hg37.csv delete mode 100644 assets/svync/default.yaml create mode 100644 null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt create mode 100644 null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt diff --git a/assets/samplesheet_HG002.csv b/assets/samplesheet_HG002.csv deleted file mode 100644 index 10f67ed..0000000 --- a/assets/samplesheet_HG002.csv +++ /dev/null @@ -1,5 +0,0 @@ -test_vcf,caller,vartype -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_delly_SV_hg19.vcf.gz",delly,sv -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_lumpy_SV_hg19.sorted.vcf.gz",lumpy,sv -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/dragen_paper/HG002_manta_SV_hg19_genotype2.vcf",manta,sv -"/Users/w620-admin/Desktop/nf-core/dataset/hg37/Broad_svaba_05052017/full.svaba.germline.sv.vcf",svaba,sv diff --git a/assets/samplesheet_HG002_hg37.csv b/assets/samplesheet_HG002_hg37.csv new file mode 100644 index 0000000..c711b62 --- /dev/null +++ b/assets/samplesheet_HG002_hg37.csv @@ -0,0 +1,5 @@ +test_vcf,caller,vartype +"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_delly_SV_hg19.chr21.vcf.gz",delly,sv +"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_lumpy_SV_hg19.sorted.chr21.vcf.gz",lumpy,sv +"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_manta_SV_hg19_genotype.chr21.vcf",manta,sv +"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/full.svaba.germline.sv.chr21.vcf",svaba,sv diff --git a/assets/samplesheet_HG002_hg38.csv b/assets/samplesheet_HG002_hg38.csv index 06171dc..c080639 100644 --- a/assets/samplesheet_HG002_hg38.csv +++ b/assets/samplesheet_HG002_hg38.csv @@ -1,7 +1,4 @@ test_vcf,caller,vartype -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.vcf.gz",lumpy,sv -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/GIAB_GRCh38_SVs_06252018/manta.HG002.vcf.gz",manta,sv -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/Ashkenazim_unnanotated/Ashkenazim_HG002.filtered.sv.vcf.gz",merged,sv -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_DRAGEN_SV_hg19.vcf.gz",dragen,sv - - +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.chr21.vcf.gz,lumpy,sv +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,sv +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,sv diff --git a/assets/samplesheet_SEQC2.csv b/assets/samplesheet_SEQC2.csv index 5b64aed..7edfb69 100644 --- a/assets/samplesheet_SEQC2.csv +++ b/assets/samplesheet_SEQC2.csv @@ -1,3 +1,3 @@ test_vcf,caller -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/test/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-Strelka.indel.vcf.gz",strelka -"/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/test/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-MuTect2.vcf.gz",mutect2 +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/test/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-Strelka.indel.vcf.gz",strelka,sv +"/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/test/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-MuTect2.vcf.gz",mutect2,sv diff --git a/assets/svync/default.yaml b/assets/svync/default.yaml deleted file mode 100644 index e69de29..0000000 diff --git a/conf/modules.config b/conf/modules.config index 802adfd..50e4f87 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -36,7 +36,7 @@ process { mode: params.publish_dir_mode ] } - withName: "BCFTOOLS_NORM_2" { + withName: "BCFTOOLS_NORM_2" { ext.prefix = {"${params.sample}"} ext.args = {"--output-type z --rm-du exact -c w" } ext.prefix = {"${meta.id}_dedup"} diff --git a/conf/test.config b/conf/test.config index 5500d37..4732563 100644 --- a/conf/test.config +++ b/conf/test.config @@ -21,7 +21,7 @@ params { // Input data sample = "HG002" - input = 'assets/samplesheet.csv' + input = "assets/samplesheet_HG002_hg38.csv" // Genome references genome = 'hg38' @@ -30,8 +30,9 @@ params { analysis = 'germline' method = 'truvari,svanalyzer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. - //preprocess = "normalization, deduplication" + preprocess = "normalization, deduplication" min_sv_size = 0 + standardization = true - truth = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" + truth = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" } diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 62c2320..b4bb4bf 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -4,7 +4,7 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'quay.io/biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/nextflow_schema.json b/nextflow_schema.json index 25fb870..a7a3199 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -42,7 +42,7 @@ "fa_icon": "fas fa-folder-open" }, "standardization": { - "type": "string", + "type": "boolean", "format": "directory-path", "description": "", "fa_icon": "fas fa-folder-open" @@ -54,7 +54,7 @@ "fa_icon": "fas fa-folder-open" }, "min_sv_size": { - "type": "string", + "type": "integer", "format": "directory-path", "description": "", "fa_icon": "fas fa-folder-open" diff --git a/null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt b/null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt new file mode 100644 index 0000000..6b739ac --- /dev/null +++ b/null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt @@ -0,0 +1 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt b/null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt new file mode 100644 index 0000000..6b739ac --- /dev/null +++ b/null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt @@ -0,0 +1 @@ +task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 153f477..2ec4bef 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -11,8 +11,9 @@ include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' workflow SV_VCF_CONVERSIONS { take: - input_ch // channel: [val(meta), vcf, config.yml] + input_ch // channel: [val(meta), vcf] ref // reference channel [ref.fa, ref.fa.fai] + svync_yaml // yaml configs main: versions = Channel.empty() @@ -23,7 +24,7 @@ workflow SV_VCF_CONVERSIONS { // zip and index input test files BGZIP_TABIX( - input_ch.map{it -> tuple(it[0], it[1])} + input_ch ) versions = versions.mix(BGZIP_TABIX.out.versions) vcf_ch = BGZIP_TABIX.out.gz_tbi @@ -35,18 +36,21 @@ workflow SV_VCF_CONVERSIONS { if(params.standardization){ out_vcf_ch = Channel.empty() - input_ch.map{it -> tuple(it[0], it[2])} - .combine(vcf_ch, by:0) - .map{it -> tuple(it[0], it[2], it[3], it[1])} - .set{snd_ch} - - snd_ch.branch{ + vcf_ch.branch{ tool: it[0].id == "delly" || it[0].id == "gridss" || it[0].id == "manta" || it[0].id == "smoove" other: true} .set{input} + svync_yaml.flatten() + .map { it -> tuple([id: it.getSimpleName(), vartype: "sv"], it) } + .set{config} + + input.tool + .combine(config, by:0) + .set{svync_ch} + SVYNC( - input.tool + svync_ch ) out_vcf_ch = out_vcf_ch.mix(SVYNC.out.vcf) out_vcf_ch = out_vcf_ch.mix(input.other) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 095e119..a8ec2ac 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -60,8 +60,10 @@ workflow VARIANTBENCHMARKING { high_conf = params.high_conf ? Channel.fromPath(params.high_conf, checkIfExists: true).collect() : Channel.empty() - // TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID + svync_yaml = params.standardization ? Channel.fromPath("assets/svync/*.yaml").collect() + : Channel.empty() + // TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID ch_samplesheet.branch{ sv: it[0].vartype == "sv" small: it[0].vartype == "small" @@ -72,14 +74,14 @@ workflow VARIANTBENCHMARKING { out_vcf_ch = Channel.empty() // PREPROCESSES - // // SUBWORKFLOW: SV_VCF_CONVERSIONS // // Standardize SV VCFs, tool spesific modifications SV_VCF_CONVERSIONS( input.sv, - ref + ref, + svync_yaml ) ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) out_vcf_ch = out_vcf_ch.mix(SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])}) From 676017e8f7d32297c17ee7168bc244c1163054e8 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> Date: Fri, 23 Feb 2024 16:34:02 +0100 Subject: [PATCH 029/292] Update pyproject.toml --- pyproject.toml | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/pyproject.toml b/pyproject.toml index 63c0021..5611062 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,6 +1,6 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 target-version = "py38" cache-dir = "~/.cache/ruff" From 7bcf0460f655edf999d0e3c911c448b007a1a9e4 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 23 Feb 2024 16:48:45 +0100 Subject: [PATCH 030/292] lint fix --- nextflow.config | 2 +- nextflow_schema.json | 24 ++++++++++++++++++++++++ 2 files changed, 25 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 169628c..099be8d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -18,7 +18,7 @@ params { // normalization includes braking down multiallelic samples -m any // deduplication removes one of the variants in the same position preprocess = "" - standardization = "" + standardization = false harmonize = false high_conf = "" diff --git a/nextflow_schema.json b/nextflow_schema.json index a7a3199..143c031 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -29,6 +29,24 @@ "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", "fa_icon": "fas fa-folder-open" }, + "high_conf": { + "type": "string", + "format": "file-path", + "exists": false, + "pattern": "^\\S+\\.(bed)?(\\.gz)?$", + "description": "Path to the high confidence BED files.", + "help_text": "High confidence BED files", + "fa_icon": "fas fa-file-csv" + }, + "truth": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.(vcf)?(\\.gz)?$", + "description": "Path to the golden set VCF files.", + "help_text": "Truth or golden set VCF file, to be used for comparisons", + "fa_icon": "fas fa-file-csv" + }, "analysis": { "type": "string", "format": "directory-path", @@ -47,6 +65,12 @@ "description": "", "fa_icon": "fas fa-folder-open" }, + "hormonize": { + "type": "boolean", + "format": "directory-path", + "description": "", + "fa_icon": "fas fa-folder-open" + }, "method": { "type": "string", "format": "directory-path", From b64fda14afd27ba7d78a8949839705c6c88f3f81 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 23 Feb 2024 16:53:33 +0100 Subject: [PATCH 031/292] fix harmonize --- nextflow.config | 2 ++ nextflow_schema.json | 2 +- 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 099be8d..5cfc390 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,6 +13,8 @@ params { // Input options input = null outdir = "results" + truth = null + high_conf = null // Preprocess spesific parameters // normalization includes braking down multiallelic samples -m any diff --git a/nextflow_schema.json b/nextflow_schema.json index 143c031..12700cb 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -65,7 +65,7 @@ "description": "", "fa_icon": "fas fa-folder-open" }, - "hormonize": { + "harmonize": { "type": "boolean", "format": "directory-path", "description": "", From 94a087733f80328e92a36ae98f9adea84e9efed0 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 29 Feb 2024 16:13:45 +0000 Subject: [PATCH 032/292] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +---- .github/CONTRIBUTING.md | 14 ++++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .gitpod.yml | 6 +-- README.md | 3 +- modules.json | 8 ++-- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +++--- .../main.nf | 10 +++-- .../tests/main.function.nf.test | 2 +- .../tests/main.function.nf.test.snap | 12 +++++- .../tests/main.workflow.nf.test | 20 ++------- .../tests/nextflow.config | 2 +- .../tests/main.function.nf.test.snap | 42 +++++++++++++++---- .../tests/main.workflow.nf.test.snap | 6 ++- .../tests/main.nf.test | 2 +- 23 files changed, 99 insertions(+), 74 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe..b290e09 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 71ac0c0..e4be8b3 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/variantbenchmarking then the best place to ask is on the nf-core Slack [#variantbenchmarking](https://nfcore.slack.com/channels/variantbenchmarking) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/variantbenchmarking then the best place to ask is on the nf-core Slack [#variantbenchmarking](https://nfcore.slack.com/channels/variantbenchmarking) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index b880d5e..ecc78ac 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/vari - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/variantbenchmarking/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/variantbenchmarking _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 11820a8..ae0bf81 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index f9e9e20..8c2110e 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 4724054..0a0ffd3 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index f823210..08622fd 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b431..073e187 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c3674af..d468aea 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.gitpod.yml b/.gitpod.yml index 363d5b1..105a182 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -10,13 +10,11 @@ tasks: vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/README.md b/README.md index e82ef33..7630b8d 100644 --- a/README.md +++ b/README.md @@ -7,12 +7,13 @@ [![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/variantbenchmarking) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/variantbenchmarking) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/modules.json b/modules.json index c536282..c1d227f 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 2212096..ca39fb6 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 354f443..47ac352 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b48..bfebd80 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index dc90ad9..699292a 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// Subworkflow with functionality specific to the nf-core/variantbenchmarking pipeline // /* @@ -152,7 +152,9 @@ workflow PIPELINE_COMPLETION { // def validateInputParameters() { genomeExistsError() -}// +} + +// // Validate channels from input samplesheet // def validateInputSamplesheet(input) { @@ -190,7 +192,9 @@ def genomeExistsError() { "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" error(error_string) } -}// +} + +// // Generate methods description for MultiQC // def toolCitationText() { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test index 8ed4310..68718e4 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -51,4 +51,4 @@ nextflow_function { ) } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap index db2030f..e3f0baf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -3,10 +3,18 @@ "content": [ "v9.9.9" ], - "timestamp": "2024-01-19T11:32:36.031083" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" }, "Test Function checkCondaChannels": { "content": null, - "timestamp": "2024-01-19T11:32:50.456" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index f7c54bc..ca964ce 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -11,9 +11,6 @@ nextflow_workflow { test("Should run no inputs") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = false @@ -39,9 +36,6 @@ nextflow_workflow { test("Should print version") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = true @@ -68,19 +62,16 @@ nextflow_workflow { test("Should dump params") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = 'results' check_conda_channels = false input[0] = false input[1] = true - input[2] = params.outdir + input[2] = outdir input[3] = false """ } @@ -96,19 +87,16 @@ nextflow_workflow { test("Should not create params JSON if no output directory") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = null check_conda_channels = false input[0] = false input[1] = true - input[2] = null + input[2] = outdir input[3] = false """ } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 53574ff..d0a926b 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -6,4 +6,4 @@ manifest { nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 10f948e..1037232 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -1,25 +1,41 @@ { "Test Function checkProfileProvided": { "content": null, - "timestamp": "2024-02-09T15:43:55.145717" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" }, "Test Function checkConfigProvided": { "content": [ true ], - "timestamp": "2024-01-19T11:34:13.548431224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" }, "Test Function nfCoreLogo": { "content": [ "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" ], - "timestamp": "2024-01-19T11:34:38.840454873" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" }, "Test Function workflowCitation": { "content": [ "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" ], - "timestamp": "2024-01-19T11:34:22.24352016" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" }, "Test Function without logColours": { "content": [ @@ -73,13 +89,21 @@ "biwhite": "" } ], - "timestamp": "2024-01-19T11:35:04.418416984" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" }, "Test Function dashedLine": { "content": [ "-\u001b[2m----------------------------------------------------\u001b[0m-" ], - "timestamp": "2024-01-19T11:34:55.420000755" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" }, "Test Function with logColours": { "content": [ @@ -133,6 +157,10 @@ "biwhite": "\u001b[1;97m" } ], - "timestamp": "2024-01-19T11:35:13.436366565" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap index d07ce54..859d103 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -10,6 +10,10 @@ ] } ], - "timestamp": "2024-01-19T11:35:22.538940073" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test index 517ee54..5784a33 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -197,4 +197,4 @@ nextflow_workflow { ) } } -} \ No newline at end of file +} From ddc4a1c293881ec20cfd11a0ef2172f4ff9a3e92 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 4 Mar 2024 15:05:42 +0000 Subject: [PATCH 033/292] modules revert to updated versions --- conf/test.config | 2 +- modules.json | 80 +------ modules/local/bcftools_view.nf | 2 +- modules/local/bgzip_tabix.nf | 2 +- modules/local/gridss_annotation.nf | 4 +- modules/local/vcf_genotype_annotator.nf | 2 +- .../nf-core/bcftools/annotate/environment.yml | 7 - modules/nf-core/bcftools/annotate/main.nf | 63 ----- modules/nf-core/bcftools/annotate/meta.yml | 56 ----- modules/nf-core/bcftools/isec/environment.yml | 7 - modules/nf-core/bcftools/isec/main.nf | 33 --- modules/nf-core/bcftools/isec/meta.yml | 52 ----- .../nf-core/bcftools/isec/tests/main.nf.test | 44 ---- .../bcftools/isec/tests/main.nf.test.snap | 25 -- .../bcftools/isec/tests/nextflow.config | 3 - modules/nf-core/bcftools/isec/tests/tags.yml | 2 - modules/nf-core/bcftools/norm/environment.yml | 2 +- modules/nf-core/bcftools/norm/main.nf | 17 +- modules/nf-core/bcftools/reheader/main.nf | 18 +- modules/nf-core/bcftools/sort/environment.yml | 7 - modules/nf-core/bcftools/sort/main.nf | 63 ----- modules/nf-core/bcftools/sort/meta.yml | 42 ---- modules/nf-core/bcftools/stats/main.nf | 16 +- modules/nf-core/bcftools/view/environment.yml | 7 - modules/nf-core/bcftools/view/main.nf | 55 ----- modules/nf-core/bcftools/view/meta.yml | 64 ------ .../nf-core/bcftools/view/tests/main.nf.test | 103 --------- .../bcftools/view/tests/main.nf.test.snap | 45 ---- .../bcftools/view/tests/nextflow.config | 3 - modules/nf-core/bcftools/view/tests/tags.yml | 2 - modules/nf-core/bedgovcf/environment.yml | 7 - modules/nf-core/bedgovcf/main.nf | 49 ---- modules/nf-core/bedgovcf/meta.yml | 57 ----- modules/nf-core/bedgovcf/tests/main.nf.test | 77 ------- .../nf-core/bedgovcf/tests/main.nf.test.snap | 60 ----- modules/nf-core/bedgovcf/tests/tags.yml | 2 - .../nf-core/bedtools/coverage/environment.yml | 7 - modules/nf-core/bedtools/coverage/main.nf | 39 ---- modules/nf-core/bedtools/coverage/meta.yml | 57 ----- .../bedtools/genomecov/environment.yml | 7 - modules/nf-core/bedtools/genomecov/main.nf | 70 ------ modules/nf-core/bedtools/genomecov/meta.yml | 59 ----- .../bedtools/intersect/environment.yml | 7 - modules/nf-core/bedtools/intersect/main.nf | 59 ----- modules/nf-core/bedtools/intersect/meta.yml | 59 ----- .../nf-core/bedtools/merge/environment.yml | 7 - modules/nf-core/bedtools/merge/main.nf | 47 ---- modules/nf-core/bedtools/merge/meta.yml | 45 ---- .../nf-core/bedtools/subtract/environment.yml | 7 - modules/nf-core/bedtools/subtract/main.nf | 50 ---- modules/nf-core/bedtools/subtract/meta.yml | 45 ---- .../bedtools/unionbedg/environment.yml | 7 - modules/nf-core/bedtools/unionbedg/main.nf | 50 ---- modules/nf-core/bedtools/unionbedg/meta.yml | 52 ----- modules/nf-core/fastqc/environment.yml | 7 - modules/nf-core/fastqc/main.nf | 55 ----- modules/nf-core/fastqc/meta.yml | 57 ----- modules/nf-core/fastqc/tests/main.nf.test | 212 ----------------- .../nf-core/fastqc/tests/main.nf.test.snap | 88 ------- modules/nf-core/fastqc/tests/tags.yml | 2 - .../manta/convertinversion/environment.yml | 9 + .../nf-core/manta/convertinversion/main.nf | 22 +- .../nf-core/manta/convertinversion/meta.yml | 4 +- modules/nf-core/multiqc/environment.yml | 4 +- modules/nf-core/multiqc/main.nf | 8 +- modules/nf-core/multiqc/meta.yml | 3 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +- modules/nf-core/survivor/filter/main.nf | 2 +- modules/nf-core/survivor/stats/main.nf | 20 +- .../survivor/stats/tests/main.nf.test.snap | 6 +- .../nf-core/svanalyzer/svbenchmark/main.nf | 2 +- modules/nf-core/svync/main.nf | 23 +- modules/nf-core/svync/meta.yml | 4 - modules/nf-core/svync/tests/main.nf.test | 35 +-- modules/nf-core/tabix/bgzip/environment.yml | 1 + modules/nf-core/tabix/bgzip/main.nf | 13 +- .../tabix/bgzip/tests/bgzip_compress.config | 5 + .../nf-core/tabix/bgzip/tests/main.nf.test | 111 +++++++++ .../tabix/bgzip/tests/main.nf.test.snap | 186 +++++++++++++++ modules/nf-core/tabix/bgzip/tests/tags.yml | 2 + .../nf-core/tabix/bgzip/tests/vcf_none.config | 5 + .../nf-core/tabix/bgziptabix/environment.yml | 1 + modules/nf-core/tabix/bgziptabix/main.nf | 12 +- .../tabix/bgziptabix/tests/main.nf.test | 94 ++++++++ .../tabix/bgziptabix/tests/main.nf.test.snap | 143 ++++++++++++ .../tabix/bgziptabix/tests/tabix_csi.config | 5 + .../tabix/bgziptabix/tests/tabix_tbi.config | 5 + .../nf-core/tabix/bgziptabix/tests/tags.yml | 2 + modules/nf-core/tabix/tabix/environment.yml | 1 + modules/nf-core/tabix/tabix/main.nf | 13 +- .../nf-core/tabix/tabix/tests/main.nf.test | 142 ++++++++++++ .../tabix/tabix/tests/main.nf.test.snap | 217 ++++++++++++++++++ .../tabix/tabix/tests/tabix_bed.config | 5 + .../tabix/tabix/tests/tabix_gff.config | 5 + .../tabix/tabix/tests/tabix_vcf_csi.config | 5 + .../tabix/tabix/tests/tabix_vcf_tbi.config | 5 + modules/nf-core/tabix/tabix/tests/tags.yml | 2 + nextflow.config | 14 +- nextflow_schema.json | 6 +- .../execution_trace_2024-02-23_11-23-40.txt | 1 - .../execution_trace_2024-02-23_11-25-34.txt | 1 - subworkflows/local/prepare_vcfs_test.nf | 17 +- subworkflows/local/prepare_vcfs_truth.nf | 19 +- subworkflows/local/report_vcf_statistics.nf | 2 +- subworkflows/local/sv_vcf_conversion.nf | 13 +- 105 files changed, 1137 insertions(+), 2245 deletions(-) delete mode 100644 modules/nf-core/bcftools/annotate/environment.yml delete mode 100644 modules/nf-core/bcftools/annotate/main.nf delete mode 100644 modules/nf-core/bcftools/annotate/meta.yml delete mode 100644 modules/nf-core/bcftools/isec/environment.yml delete mode 100644 modules/nf-core/bcftools/isec/main.nf delete mode 100644 modules/nf-core/bcftools/isec/meta.yml delete mode 100644 modules/nf-core/bcftools/isec/tests/main.nf.test delete mode 100644 modules/nf-core/bcftools/isec/tests/main.nf.test.snap delete mode 100644 modules/nf-core/bcftools/isec/tests/nextflow.config delete mode 100644 modules/nf-core/bcftools/isec/tests/tags.yml delete mode 100644 modules/nf-core/bcftools/sort/environment.yml delete mode 100644 modules/nf-core/bcftools/sort/main.nf delete mode 100644 modules/nf-core/bcftools/sort/meta.yml delete mode 100644 modules/nf-core/bcftools/view/environment.yml delete mode 100644 modules/nf-core/bcftools/view/main.nf delete mode 100644 modules/nf-core/bcftools/view/meta.yml delete mode 100644 modules/nf-core/bcftools/view/tests/main.nf.test delete mode 100644 modules/nf-core/bcftools/view/tests/main.nf.test.snap delete mode 100644 modules/nf-core/bcftools/view/tests/nextflow.config delete mode 100644 modules/nf-core/bcftools/view/tests/tags.yml delete mode 100644 modules/nf-core/bedgovcf/environment.yml delete mode 100644 modules/nf-core/bedgovcf/main.nf delete mode 100644 modules/nf-core/bedgovcf/meta.yml delete mode 100644 modules/nf-core/bedgovcf/tests/main.nf.test delete mode 100644 modules/nf-core/bedgovcf/tests/main.nf.test.snap delete mode 100644 modules/nf-core/bedgovcf/tests/tags.yml delete mode 100644 modules/nf-core/bedtools/coverage/environment.yml delete mode 100644 modules/nf-core/bedtools/coverage/main.nf delete mode 100644 modules/nf-core/bedtools/coverage/meta.yml delete mode 100644 modules/nf-core/bedtools/genomecov/environment.yml delete mode 100644 modules/nf-core/bedtools/genomecov/main.nf delete mode 100644 modules/nf-core/bedtools/genomecov/meta.yml delete mode 100644 modules/nf-core/bedtools/intersect/environment.yml delete mode 100644 modules/nf-core/bedtools/intersect/main.nf delete mode 100644 modules/nf-core/bedtools/intersect/meta.yml delete mode 100644 modules/nf-core/bedtools/merge/environment.yml delete mode 100644 modules/nf-core/bedtools/merge/main.nf delete mode 100644 modules/nf-core/bedtools/merge/meta.yml delete mode 100644 modules/nf-core/bedtools/subtract/environment.yml delete mode 100644 modules/nf-core/bedtools/subtract/main.nf delete mode 100644 modules/nf-core/bedtools/subtract/meta.yml delete mode 100644 modules/nf-core/bedtools/unionbedg/environment.yml delete mode 100644 modules/nf-core/bedtools/unionbedg/main.nf delete mode 100644 modules/nf-core/bedtools/unionbedg/meta.yml delete mode 100644 modules/nf-core/fastqc/environment.yml delete mode 100644 modules/nf-core/fastqc/main.nf delete mode 100644 modules/nf-core/fastqc/meta.yml delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test delete mode 100644 modules/nf-core/fastqc/tests/main.nf.test.snap delete mode 100644 modules/nf-core/fastqc/tests/tags.yml create mode 100644 modules/nf-core/manta/convertinversion/environment.yml create mode 100644 modules/nf-core/tabix/bgzip/tests/bgzip_compress.config create mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test create mode 100644 modules/nf-core/tabix/bgzip/tests/main.nf.test.snap create mode 100644 modules/nf-core/tabix/bgzip/tests/tags.yml create mode 100644 modules/nf-core/tabix/bgzip/tests/vcf_none.config create mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test create mode 100644 modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config create mode 100644 modules/nf-core/tabix/bgziptabix/tests/tags.yml create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test create mode 100644 modules/nf-core/tabix/tabix/tests/main.nf.test.snap create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_bed.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_gff.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config create mode 100644 modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config create mode 100644 modules/nf-core/tabix/tabix/tests/tags.yml delete mode 100644 null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt delete mode 100644 null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt diff --git a/conf/test.config b/conf/test.config index 4732563..35a66c1 100644 --- a/conf/test.config +++ b/conf/test.config @@ -31,7 +31,7 @@ params { method = 'truvari,svanalyzer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. preprocess = "normalization, deduplication" - min_sv_size = 0 + min_sv_size = 50 standardization = true truth = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" diff --git a/modules.json b/modules.json index cd71108..7fb2bb8 100644 --- a/modules.json +++ b/modules.json @@ -5,89 +5,29 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "bcftools/annotate": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "bcftools/isec": { - "branch": "master", - "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", - "installed_by": ["modules"] - }, "bcftools/norm": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/reheader": { "branch": "master", - "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", - "installed_by": ["modules"] - }, - "bcftools/sort": { - "branch": "master", - "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "bcftools/stats": { "branch": "master", - "git_sha": "41dfa13929d2c178855159a69d2e2958c52be155", - "installed_by": ["modules"] - }, - "bcftools/view": { - "branch": "master", - "git_sha": "682f789f93070bd047868300dd018faf3d434e7c", - "installed_by": ["modules"] - }, - "bedgovcf": { - "branch": "master", - "git_sha": "338a5572efbda56110cea2f657615920d8dce58c", - "installed_by": ["modules"] - }, - "bedtools/coverage": { - "branch": "master", - "git_sha": "1d1cb7bfef6cf67fbc7faafa6992ad8bdc3045b3", - "installed_by": ["modules"] - }, - "bedtools/genomecov": { - "branch": "master", - "git_sha": "90aef30f432332bdf0ce9f4b9004aa5d5c4960bb", - "installed_by": ["modules"] - }, - "bedtools/intersect": { - "branch": "master", - "git_sha": "90aef30f432332bdf0ce9f4b9004aa5d5c4960bb", - "installed_by": ["modules"] - }, - "bedtools/merge": { - "branch": "master", - "git_sha": "90aef30f432332bdf0ce9f4b9004aa5d5c4960bb", - "installed_by": ["modules"] - }, - "bedtools/subtract": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "bedtools/unionbedg": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] - }, - "fastqc": { - "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, "manta/convertinversion": { "branch": "master", - "git_sha": "b8ff8e32e305587ac2c7909db3058fbbba2a859a", + "git_sha": "1e2b7fb7106852388610c0360d234b0829eb980e", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] }, "survivor/filter": { @@ -97,7 +37,7 @@ }, "survivor/stats": { "branch": "master", - "git_sha": "00d03755abd2cac54a039525716aeabea9efb9e7", + "git_sha": "398375d72766cac92c4ffcf6927cac2d65310cfd", "installed_by": ["modules"] }, "svanalyzer/svbenchmark": { @@ -107,22 +47,22 @@ }, "svync": { "branch": "master", - "git_sha": "49925b34411ab0aef3fdb61a4d516ef36eceec05", + "git_sha": "3df1a372b844c98c31ec3446faac7c75e64518a3", "installed_by": ["modules"] }, "tabix/bgzip": { "branch": "master", - "git_sha": "31c0b49f6527ef196e89eca49a36af2de71711f8", + "git_sha": "09d3c8c29b31a2dfd610305b10550f0e1dbcd4a9", "installed_by": ["modules"] }, "tabix/bgziptabix": { "branch": "master", - "git_sha": "5e7b1ef9a5a2d9258635bcbf70fcf37dacd1b247", + "git_sha": "09d3c8c29b31a2dfd610305b10550f0e1dbcd4a9", "installed_by": ["modules"] }, "tabix/tabix": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "09d3c8c29b31a2dfd610305b10550f0e1dbcd4a9", "installed_by": ["modules"] }, "truvari/bench": { diff --git a/modules/local/bcftools_view.nf b/modules/local/bcftools_view.nf index 71e1fa2..7b52d86 100644 --- a/modules/local/bcftools_view.nf +++ b/modules/local/bcftools_view.nf @@ -5,7 +5,7 @@ process BCFTOOLS_VIEW { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: tuple val(meta),path(vcf), path(index) diff --git a/modules/local/bgzip_tabix.nf b/modules/local/bgzip_tabix.nf index f7f32fb..986eae4 100644 --- a/modules/local/bgzip_tabix.nf +++ b/modules/local/bgzip_tabix.nf @@ -5,7 +5,7 @@ process BGZIP_TABIX { conda "" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + 'biocontainers/tabix:1.11--hdfd78af_0' }" input: tuple val(meta),path(vcf) diff --git a/modules/local/gridss_annotation.nf b/modules/local/gridss_annotation.nf index 523d4bb..4e0a090 100644 --- a/modules/local/gridss_annotation.nf +++ b/modules/local/gridss_annotation.nf @@ -6,10 +6,10 @@ process GRIDSS_ANNOTATION { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gridss:2.13.2--h270b39a_0': - 'quay.io/biocontainers/gridss:2.13.2--h270b39a_0' }" + 'biocontainers/gridss:2.13.2--h270b39a_0' }" input: - tuple val(meta),path(vcf), path(index) + tuple val(meta), path(vcf), path(index) tuple path(fasta), path(fasta_fai) output: diff --git a/modules/local/vcf_genotype_annotator.nf b/modules/local/vcf_genotype_annotator.nf index 9fa366a..cd59090 100644 --- a/modules/local/vcf_genotype_annotator.nf +++ b/modules/local/vcf_genotype_annotator.nf @@ -4,7 +4,7 @@ process VCF_GENOTYPE_ANNOTATOR { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'ddocker://griffithlab/vatools:5.1.10' + 'docker://griffithlab/vatools:5.1.10' 'griffithlab/vatools:5.1.10' }" input: diff --git a/modules/nf-core/bcftools/annotate/environment.yml b/modules/nf-core/bcftools/annotate/environment.yml deleted file mode 100644 index 273ffff..0000000 --- a/modules/nf-core/bcftools/annotate/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bcftools_annotate -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bcftools=1.17 diff --git a/modules/nf-core/bcftools/annotate/main.nf b/modules/nf-core/bcftools/annotate/main.nf deleted file mode 100644 index 05b9c63..0000000 --- a/modules/nf-core/bcftools/annotate/main.nf +++ /dev/null @@ -1,63 +0,0 @@ -process BCFTOOLS_ANNOTATE { - tag "$meta.id" - label 'process_low' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" - - input: - tuple val(meta), path(input), path(index), path(annotations), path(annotations_index), path(header_lines) - - output: - tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def header_file = header_lines ? "--header-lines ${header_lines}" : '' - def annotations_file = annotations ? "--annotations ${annotations}" : '' - def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : - args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : - args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : - args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : - "vcf" - if ("$input" == "${prefix}.${extension}") error "Input and output names are the same, set prefix in module configuration to disambiguate!" - """ - bcftools \\ - annotate \\ - $args \\ - $annotations_file \\ - $header_file \\ - --output ${prefix}.${extension} \\ - --threads $task.cpus \\ - $input - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : - args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : - args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : - args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : - "vcf" - """ - touch ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/bcftools/annotate/meta.yml b/modules/nf-core/bcftools/annotate/meta.yml deleted file mode 100644 index f3aa463..0000000 --- a/modules/nf-core/bcftools/annotate/meta.yml +++ /dev/null @@ -1,56 +0,0 @@ -name: bcftools_annotate -description: Add or remove annotations. -keywords: - - bcftools - - annotate - - vcf - - remove - - add -tools: - - annotate: - description: Add or remove annotations. - homepage: http://samtools.github.io/bcftools/bcftools.html - documentation: https://samtools.github.io/bcftools/bcftools.html#annotate - doi: 10.1093/bioinformatics/btp352 - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - input: - type: file - description: Query VCF or BCF file, can be either uncompressed or compressed - - index: - type: file - description: Index of the query VCF or BCF file - - annotations: - type: file - description: Bgzip-compressed file with annotations - - annotations_index: - type: file - description: Index of the annotations file - - header_lines: - type: file - description: Contains lines to append to the output VCF header -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - vcf: - type: file - description: Compressed annotated VCF file - pattern: "*{vcf,vcf.gz,bcf,bcf.gz}" -authors: - - "@projectoriented" - - "@ramprasadn" -maintainers: - - "@projectoriented" - - "@ramprasadn" diff --git a/modules/nf-core/bcftools/isec/environment.yml b/modules/nf-core/bcftools/isec/environment.yml deleted file mode 100644 index e798a40..0000000 --- a/modules/nf-core/bcftools/isec/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bcftools_isec -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/isec/main.nf b/modules/nf-core/bcftools/isec/main.nf deleted file mode 100644 index 64aae32..0000000 --- a/modules/nf-core/bcftools/isec/main.nf +++ /dev/null @@ -1,33 +0,0 @@ -process BCFTOOLS_ISEC { - tag "$meta.id $meta.caller" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'biocontainers/bcftools:1.18--h8b25389_0' }" - - input: - tuple val(meta),val(meta2), path(vcfs), path(tbis) - - output: - tuple val(meta),val(meta2), path("${prefix}"), emit: results - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - prefix = task.ext.prefix ?: "${meta.id}" - """ - bcftools isec \\ - $args \\ - -p $prefix \\ - *.vcf.gz - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/bcftools/isec/meta.yml b/modules/nf-core/bcftools/isec/meta.yml deleted file mode 100644 index 94f4256..0000000 --- a/modules/nf-core/bcftools/isec/meta.yml +++ /dev/null @@ -1,52 +0,0 @@ -name: bcftools_isec -description: Apply set operations to VCF files -keywords: - - variant calling - - intersect - - union - - complement - - VCF -tools: - - isec: - description: | - Computes intersections, unions and complements of VCF files. - homepage: http://samtools.github.io/bcftools/bcftools.html - documentation: http://www.htslib.org/doc/bcftools.html - doi: 10.1093/bioinformatics/btp352 - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcfs: - type: list - description: | - List containing 2 or more vcf files - e.g. [ 'file1.vcf', 'file2.vcf' ] - - tbis: - type: list - description: | - List containing the tbi index files corresponding to the vcfs input files - e.g. [ 'file1.vcf.tbi', 'file2.vcf.tbi' ] -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - results: - type: directory - description: Folder containing the set operations results perform on the vcf files - pattern: "${prefix}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@joseespinosa" - - "@drpatelh" -maintainers: - - "@joseespinosa" - - "@drpatelh" diff --git a/modules/nf-core/bcftools/isec/tests/main.nf.test b/modules/nf-core/bcftools/isec/tests/main.nf.test deleted file mode 100644 index 89d5335..0000000 --- a/modules/nf-core/bcftools/isec/tests/main.nf.test +++ /dev/null @@ -1,44 +0,0 @@ -nextflow_process { - - name "Test Process BCFTOOLS_ISEC" - script "../main.nf" - process "BCFTOOLS_ISEC" - - tag "modules" - tag "modules_nfcore" - tag "bcftools" - tag "bcftools/isec" - - config "./nextflow.config" - - test("sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]]") { - - when { - process { - """ - input[0] = [ - [ id:'test' ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_vcf_gz'], checkIfExists: true)], - [ - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_vcf_gz_tbi'], checkIfExists: true)] - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.results, - process.out.versions - ).match() } - ) - } - - } - -} diff --git a/modules/nf-core/bcftools/isec/tests/main.nf.test.snap b/modules/nf-core/bcftools/isec/tests/main.nf.test.snap deleted file mode 100644 index 919809b..0000000 --- a/modules/nf-core/bcftools/isec/tests/main.nf.test.snap +++ /dev/null @@ -1,25 +0,0 @@ -{ - "sarscov2 - [[vcf1, vcf2], [tbi1, tbi2]]": { - "content": [ - [ - [ - { - "id": "test" - }, - [ - "0000.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee", - "0000.vcf.gz.tbi:md5,7f005943c935f2b55ba3f9d4802aa09f", - "0001.vcf.gz:md5,5937e33da388e9f6992ea0b44c5c2629", - "0001.vcf.gz.tbi:md5,2cdcee9edc71f6d84325d34d78d445cc", - "README.txt:md5,10fc33b66522645600d44afbd41fb792", - "sites.txt:md5,1cea3fbde7f6d3c97f3d39036f9690df" - ] - ] - ], - [ - "versions.yml:md5,c9e98dd502f38110979feabbee9937df" - ] - ], - "timestamp": "2023-11-29T14:00:22.623656731" - } -} \ No newline at end of file diff --git a/modules/nf-core/bcftools/isec/tests/nextflow.config b/modules/nf-core/bcftools/isec/tests/nextflow.config deleted file mode 100644 index 3212fe9..0000000 --- a/modules/nf-core/bcftools/isec/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args = '--nfiles +2 --output-type z --no-version' -} \ No newline at end of file diff --git a/modules/nf-core/bcftools/isec/tests/tags.yml b/modules/nf-core/bcftools/isec/tests/tags.yml deleted file mode 100644 index c0fb5a1..0000000 --- a/modules/nf-core/bcftools/isec/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -bcftools/isec: - - "modules/nf-core/bcftools/isec/**" diff --git a/modules/nf-core/bcftools/norm/environment.yml b/modules/nf-core/bcftools/norm/environment.yml index 74e94f2..fe80e4e 100644 --- a/modules/nf-core/bcftools/norm/environment.yml +++ b/modules/nf-core/bcftools/norm/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::bcftools=1.17 + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/norm/main.nf b/modules/nf-core/bcftools/norm/main.nf index 5127bba..47d3dab 100644 --- a/modules/nf-core/bcftools/norm/main.nf +++ b/modules/nf-core/bcftools/norm/main.nf @@ -4,30 +4,28 @@ process BCFTOOLS_NORM { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta), path(vcf), path(index) - tuple path(fasta), path(fai) - tuple val(meta3), path(regions) + tuple val(meta), path(vcf), path(tbi) + tuple val(meta2), path(fasta) output: - tuple val(meta),path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: def args = task.ext.args ?: '--output-type z' - def prefix = task.ext.prefix ?: "${meta.id}" + def prefix = task.ext.prefix ?: "${meta.id}" def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf.gz" - def region = regions ? "-R ${regions}" : "" """ bcftools norm \\ @@ -35,7 +33,6 @@ process BCFTOOLS_NORM { --output ${prefix}.${extension}\\ $args \\ --threads $task.cpus \\ - $region \\ ${vcf} cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/bcftools/reheader/main.nf b/modules/nf-core/bcftools/reheader/main.nf index 5f01eff..8252716 100644 --- a/modules/nf-core/bcftools/reheader/main.nf +++ b/modules/nf-core/bcftools/reheader/main.nf @@ -1,18 +1,18 @@ process BCFTOOLS_REHEADER { - tag "$meta.id " + tag "$meta.id" label 'process_low' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta),path(vcf), path(index) - tuple path(fasta), path(fai) + tuple val(meta), path(vcf), path(header), path(samples) + tuple val(meta2), path(fai) output: - tuple val(meta),path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf path "versions.yml" , emit: versions when: @@ -22,6 +22,9 @@ process BCFTOOLS_REHEADER { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def fai_argument = fai ? "--fai $fai" : "" + def header_argument = header ? "--header $header" : "" + def samples_argument = samples ? "--samples $samples" : "" + def args2 = task.ext.args2 ?: '--output-type z' def extension = args2.contains("--output-type b") || args2.contains("-Ob") ? "bcf.gz" : args2.contains("--output-type u") || args2.contains("-Ou") ? "bcf" : @@ -29,12 +32,11 @@ process BCFTOOLS_REHEADER { args2.contains("--output-type v") || args2.contains("-Ov") ? "vcf" : "vcf" """ - echo ${prefix} > sample.txt - bcftools \\ reheader \\ $fai_argument \\ - --samples sample.txt \\ + $header_argument \\ + $samples_argument \\ $args \\ --threads $task.cpus \\ $vcf \\ diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml deleted file mode 100644 index 89cf911..0000000 --- a/modules/nf-core/bcftools/sort/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bcftools_sort -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf deleted file mode 100644 index a739b09..0000000 --- a/modules/nf-core/bcftools/sort/main.nf +++ /dev/null @@ -1,63 +0,0 @@ -process BCFTOOLS_SORT { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" - - input: - tuple val(meta), val(meta2), path(vcf) - - output: - tuple val(meta), val(meta2), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf - tuple val(meta), val(meta2), path("*.{tbi}") , emit: index - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '--output-type z' - def prefix = task.ext.prefix ?: "${meta.id}" - def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : - args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : - args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : - args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : - "vcf" - - """ - bcftools \\ - sort \\ - --output ${prefix}.${extension} \\ - --temp-dir . \\ - --write-index \\ - $args \\ - $vcf - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ - - stub: - def args = task.ext.args ?: '--output-type z' - def prefix = task.ext.prefix ?: "${meta.id}" - - def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : - args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : - args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : - args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : - "vcf" - - """ - touch ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml deleted file mode 100644 index 84747c6..0000000 --- a/modules/nf-core/bcftools/sort/meta.yml +++ /dev/null @@ -1,42 +0,0 @@ -name: bcftools_sort -description: Sorts VCF files -keywords: - - sorting - - VCF - - variant calling -tools: - - sort: - description: Sort VCF files by coordinates. - homepage: http://samtools.github.io/bcftools/bcftools.html - documentation: http://www.htslib.org/doc/bcftools.html - tool_dev_url: https://github.com/samtools/bcftools - doi: "10.1093/bioinformatics/btp352" - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: The VCF/BCF file to be sorted - pattern: "*.{vcf.gz,vcf,bcf}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - vcf: - type: file - description: Sorted VCF file - pattern: "*.{vcf.gz}" -authors: - - "@Gwennid" -maintainers: - - "@Gwennid" diff --git a/modules/nf-core/bcftools/stats/main.nf b/modules/nf-core/bcftools/stats/main.nf index 2e0084b..ffa1df6 100644 --- a/modules/nf-core/bcftools/stats/main.nf +++ b/modules/nf-core/bcftools/stats/main.nf @@ -5,19 +5,19 @@ process BCFTOOLS_STATS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" + 'biocontainers/bcftools:1.18--h8b25389_0' }" input: - tuple val(meta), path(vcf), path(index) - tuple val(meta3), path(regions) - tuple val(meta4), path(targets) - tuple val(meta5), path(samples) - tuple val(meta6), path(exons) - tuple val(meta7), path(fasta) + tuple val(meta), path(vcf), path(tbi) + tuple val(meta2), path(regions) + tuple val(meta3), path(targets) + tuple val(meta4), path(samples) + tuple val(meta5), path(exons) + tuple val(meta6), path(fasta) output: tuple val(meta), path("*stats.txt"), emit: stats - path "versions.yml" , emit: versions + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/bcftools/view/environment.yml b/modules/nf-core/bcftools/view/environment.yml deleted file mode 100644 index 8937c6d..0000000 --- a/modules/nf-core/bcftools/view/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bcftools_view -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf deleted file mode 100644 index b62f56a..0000000 --- a/modules/nf-core/bcftools/view/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process BCFTOOLS_VIEW { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'quay.io/biocontainers/bcftools:1.18--h8b25389_0' }" - - input: - tuple val(meta),path(vcf), path(index) - tuple val(meta3), path(regions) - path(targets) - path(samples) - - output: - tuple val(meta), path("*.gz") , emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def regions_file = regions ? "--regions-file ${regions}" : "" - def targets_file = targets ? "--targets-file ${targets}" : "" - def samples_file = samples ? "--samples-file ${samples}" : "" - """ - bcftools view \\ - --output ${prefix}.vcf.gz \\ - ${regions_file} \\ - ${targets_file} \\ - ${samples_file} \\ - $args \\ - --threads $task.cpus \\ - ${vcf} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.vcf.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/bcftools/view/meta.yml b/modules/nf-core/bcftools/view/meta.yml deleted file mode 100644 index eaa12b5..0000000 --- a/modules/nf-core/bcftools/view/meta.yml +++ /dev/null @@ -1,64 +0,0 @@ -name: bcftools_view -description: View, subset and filter VCF or BCF files by position and filtering expression. Convert between VCF and BCF -keywords: - - variant calling - - view - - bcftools - - VCF -tools: - - view: - description: | - View, subset and filter VCF or BCF files by position and filtering expression. Convert between VCF and BCF - homepage: http://samtools.github.io/bcftools/bcftools.html - documentation: http://www.htslib.org/doc/bcftools.html - doi: 10.1093/bioinformatics/btp352 - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: | - The vcf file to be inspected. - e.g. 'file.vcf' - - index: - type: file - description: | - The tab index for the VCF file to be inspected. - e.g. 'file.tbi' - - regions: - type: file - description: | - Optionally, restrict the operation to regions listed in this file. - e.g. 'file.vcf' - - targets: - type: file - description: | - Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files) - e.g. 'file.vcf' - - samples: - type: file - description: | - Optional, file of sample names to be included or excluded. - e.g. 'file.tsv' -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: VCF normalized output file - pattern: "*.{vcf.gz}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@abhi18av" -maintainers: - - "@abhi18av" diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test b/modules/nf-core/bcftools/view/tests/main.nf.test deleted file mode 100644 index c285674..0000000 --- a/modules/nf-core/bcftools/view/tests/main.nf.test +++ /dev/null @@ -1,103 +0,0 @@ -nextflow_process { - - name "Test Process BCFTOOLS_VIEW" - script "../main.nf" - process "BCFTOOLS_VIEW" - - tag "modules" - tag "modules_nfcore" - tag "bcftools" - tag "bcftools/view" - - config "./nextflow.config" - - test("sarscov2 - [vcf, tbi], [], [], []") { - - when { - process { - """ - input[0] = [ - [ id:'out', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) - ] - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.vcf, - process.out.versions - ).match() } - ) - } - - } - - test("sarscov2 - [vcf, tbi], vcf, tsv, []") { - - when { - process { - """ - input[0] = [ - [ id:'out', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) - ] - input[1] = file(params.test_data['sarscov2']['illumina']['test3_vcf_gz'], checkIfExists: true) - input[2] = file(params.test_data['sarscov2']['illumina']['test2_vcf_targets_tsv_gz'], checkIfExists: true) - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.vcf, - process.out.versions - ).match() } - ) - } - - } - - test("sarscov2 - [vcf, tbi], [], [], [] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = [ - [ id:'out', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) - ] - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - file(process.out.vcf[0][1]).name, - process.out.versions - ).match() } - ) - } - - } - -} diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test.snap b/modules/nf-core/bcftools/view/tests/main.nf.test.snap deleted file mode 100644 index 049ac25..0000000 --- a/modules/nf-core/bcftools/view/tests/main.nf.test.snap +++ /dev/null @@ -1,45 +0,0 @@ -{ - "sarscov2 - [vcf, tbi], vcf, tsv, []": { - "content": [ - [ - [ - { - "id": "out", - "single_end": false - }, - "out.vcf.gz:md5,3c47ba1a6aa4ef9b3ad800175814d739" - ] - ], - [ - "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" - ] - ], - "timestamp": "2023-11-29T14:27:10.724842996" - }, - "sarscov2 - [vcf, tbi], [], [], [] - stub": { - "content": [ - "out.vcf.gz", - [ - "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" - ] - ], - "timestamp": "2023-11-29T14:27:17.445846794" - }, - "sarscov2 - [vcf, tbi], [], [], []": { - "content": [ - [ - [ - { - "id": "out", - "single_end": false - }, - "out.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" - ] - ], - [ - "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" - ] - ], - "timestamp": "2023-11-29T14:27:03.328392594" - } -} \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/nextflow.config b/modules/nf-core/bcftools/view/tests/nextflow.config deleted file mode 100644 index b05aa50..0000000 --- a/modules/nf-core/bcftools/view/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args = '--no-version' -} \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/tags.yml b/modules/nf-core/bcftools/view/tests/tags.yml deleted file mode 100644 index 43b1f0a..0000000 --- a/modules/nf-core/bcftools/view/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -bcftools/view: - - "modules/nf-core/bcftools/view/**" diff --git a/modules/nf-core/bedgovcf/environment.yml b/modules/nf-core/bedgovcf/environment.yml deleted file mode 100644 index 61cfbcc..0000000 --- a/modules/nf-core/bedgovcf/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bedgovcf -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bedgovcf=0.1.0 diff --git a/modules/nf-core/bedgovcf/main.nf b/modules/nf-core/bedgovcf/main.nf deleted file mode 100644 index f39f404..0000000 --- a/modules/nf-core/bedgovcf/main.nf +++ /dev/null @@ -1,49 +0,0 @@ -process BEDGOVCF { - tag "$meta.id" - label 'process_low' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedgovcf:0.1.0--h9ee0642_0': - 'biocontainers/bedgovcf:0.1.0--h9ee0642_0' }" - - input: - tuple val(meta), path(bed), path(config) - tuple val(meta2), path(fai) - - output: - tuple val(meta), path("*.vcf.gz"), emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - """ - bedgovcf \\ - $args \\ - --bed $bed \\ - --fai $fai \\ - --config $config \\ - | bgzip --stdout --threads $task.cpus $args2 > ${prefix}.vcf.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedgovcf: \$(echo \$(bedgovcf --version 2>&1) | sed 's/^bedgovcf version //' ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.vcf.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedgovcf: \$(echo \$(bedgovcf --version 2>&1) | sed 's/^bedgovcf version //' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/bedgovcf/meta.yml b/modules/nf-core/bedgovcf/meta.yml deleted file mode 100644 index 406935e..0000000 --- a/modules/nf-core/bedgovcf/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ ---- -name: "bedgovcf" -description: Convert a BED file to a VCF file according to a YAML config -keywords: - - bed - - vcf - - conversion - - variants -tools: - - "bedgovcf": - description: "A simple tool to convert BED files to VCF files" - homepage: "https://github.com/nvnieuwk/bedgovcf" - documentation: "https://github.com/nvnieuwk/bedgovcf" - tool_dev_url: "https://github.com/nvnieuwk/bedgovcf" - licence: ["MIT"] - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test', single_end:false ]` - - bed: - type: file - description: The BED file to convert to VCF - pattern: "*.bed" - - config: - type: file - description: The config file to use for the conversion - pattern: "*.{yml,yaml}" - - meta2: - type: map - description: | - Groovy Map containing fasta index information - e.g. `[ id:'test', single_end:false ]` - - fai: - type: file - description: The fasta index file - pattern: "*.fai" - -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. `[ id:'test', single_end:false ]` - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - - vcf: - type: file - description: The converted VCF file - pattern: "*.vcf.gz" - -authors: - - "@nvnieuwk" diff --git a/modules/nf-core/bedgovcf/tests/main.nf.test b/modules/nf-core/bedgovcf/tests/main.nf.test deleted file mode 100644 index f55fbb6..0000000 --- a/modules/nf-core/bedgovcf/tests/main.nf.test +++ /dev/null @@ -1,77 +0,0 @@ -nextflow_process { - - name "Test Process BEDGOVCF" - script "../main.nf" - process "BEDGOVCF" - tag "modules" - tag "modules_nfcore" - tag "bedgovcf" - - test("homo_sapiens - [bed, config] - fai") { - - // WARNING: md5sum between local tests and GHA tests differ - // Please change them manually after every snapshot update - when { - params { - outdir = $outputDir - } - process { - """ - raw_ch = Channel.of([ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) - ]) - config = Channel.of(["config.yaml", "header:", " - name: test", " content: test"]) - .collectFile(newLine:true, sort:'index') - input[0] = raw_ch.combine(config) - input[1] = [ - [id:"ref"], - file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - - ) - } - } - - - test("homo_sapiens - [bed, config] - fai STUB") { - options "-stub" - when { - params { - outdir = $outputDir - } - process { - """ - raw_ch = Channel.of([ - [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_bed'], checkIfExists: true) - ]) - config = Channel.of(["config.yaml", "header:", " - name: test", " content: test"]) - .collectFile(newLine:true, sort:'index') - input[0] = raw_ch.combine(config) - input[1] = [ - [id:"ref"], - file(params.test_data['homo_sapiens']['genome']['genome_fasta_fai'], checkIfExists: true) - ] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() } - - ) - } - } - -} diff --git a/modules/nf-core/bedgovcf/tests/main.nf.test.snap b/modules/nf-core/bedgovcf/tests/main.nf.test.snap deleted file mode 100644 index 2e34fe4..0000000 --- a/modules/nf-core/bedgovcf/tests/main.nf.test.snap +++ /dev/null @@ -1,60 +0,0 @@ -{ - "homo_sapiens - [bed, config] - fai STUB": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "1": [ - "versions.yml:md5,27e259763ddc44796207f766afb94887" - ], - "vcf": [ - [ - { - "id": "test" - }, - "test.vcf.gz:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "versions": [ - "versions.yml:md5,27e259763ddc44796207f766afb94887" - ] - } - ], - "timestamp": "2023-11-14T12:45:41.931292108" - }, - "homo_sapiens - [bed, config] - fai": { - "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" - ] - ], - "1": [ - "versions.yml:md5,27e259763ddc44796207f766afb94887" - ], - "vcf": [ - [ - { - "id": "test" - }, - "test.vcf.gz:md5,a9cb689f02723f7e0e4d674ef144b48c" - ] - ], - "versions": [ - "versions.yml:md5,27e259763ddc44796207f766afb94887" - ] - } - ], - "timestamp": "2023-11-14T12:45:36.218283171" - } -} \ No newline at end of file diff --git a/modules/nf-core/bedgovcf/tests/tags.yml b/modules/nf-core/bedgovcf/tests/tags.yml deleted file mode 100644 index d66b105..0000000 --- a/modules/nf-core/bedgovcf/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -bedgovcf: - - modules/nf-core/bedgovcf/** diff --git a/modules/nf-core/bedtools/coverage/environment.yml b/modules/nf-core/bedtools/coverage/environment.yml deleted file mode 100644 index 53a3b4c..0000000 --- a/modules/nf-core/bedtools/coverage/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bedtools_coverage -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bedtools=2.30.0 diff --git a/modules/nf-core/bedtools/coverage/main.nf b/modules/nf-core/bedtools/coverage/main.nf deleted file mode 100644 index 03b0545..0000000 --- a/modules/nf-core/bedtools/coverage/main.nf +++ /dev/null @@ -1,39 +0,0 @@ -process BEDTOOLS_COVERAGE { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--h468198e_3': - 'quay.io/biocontainers/bedtools:2.30.0--h468198e_3' }" - - input: - tuple val(meta), path(input_A), path(input_B) - path genome_file - - output: - tuple val(meta), path("*.bed"), emit: bed - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def reference = genome_file ? "-g ${genome_file} -sorted" : "" - """ - bedtools \\ - coverage \\ - $args \\ - $reference \\ - -a $input_A \\ - -b $input_B \\ - > ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(echo \$(bedtools --version 2>&1) | sed 's/^.*bedtools v//' )) - END_VERSIONS - """ -} diff --git a/modules/nf-core/bedtools/coverage/meta.yml b/modules/nf-core/bedtools/coverage/meta.yml deleted file mode 100644 index d825ca7..0000000 --- a/modules/nf-core/bedtools/coverage/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: "bedtools_coverage" -description: computes both the depth and breadth of coverage of features in file B on the features in file A -keywords: - - bedtools - - coverage - - bam - - bed - - gff - - vcf - - histogram -tools: - - "bedtools": - description: "A powerful toolset for genome arithmetic" - homepage: "https://bedtools.readthedocs.io/en/latest/index.html" - documentation: "https://bedtools.readthedocs.io/en/latest/content/bedtools-suite.html" - tool_dev_url: "https://github.com/arq5x/bedtools2" - doi: "10.1093/bioinformatics/btq033" - licence: "['GPL v2', 'MIT']" -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - input_A: - type: file - description: BAM/BED/GFF/VCF file - pattern: "*.{bam,bed,gff,vcf}" - - input_B: - type: file - description: One or more BAM/BED/GFF/VCF file - pattern: "*.{bam,bed,gff,vcf}" - - genome_file: - type: file - description: | - Optional reference genome 2 column file that defines the expected chromosome order - in the input files for use with the -sorted option. - When `genome_file` is provided, `-sorted` option is added to the command. - pattern: "*.{fai,txt,chromsizes}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bed: - type: file - description: File containing coverage of sequence alignments - pattern: "*.bed" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@priyanka-surana" -maintainers: - - "@priyanka-surana" diff --git a/modules/nf-core/bedtools/genomecov/environment.yml b/modules/nf-core/bedtools/genomecov/environment.yml deleted file mode 100644 index 574c267..0000000 --- a/modules/nf-core/bedtools/genomecov/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bedtools_genomecov -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bedtools=2.31.0 diff --git a/modules/nf-core/bedtools/genomecov/main.nf b/modules/nf-core/bedtools/genomecov/main.nf deleted file mode 100644 index 5020882..0000000 --- a/modules/nf-core/bedtools/genomecov/main.nf +++ /dev/null @@ -1,70 +0,0 @@ -process BEDTOOLS_GENOMECOV { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' : - 'quay.io/biocontainers/bedtools:2.31.0--hf5e1c6e_2' }" - - input: - tuple val(meta), path(intervals), val(scale) - path sizes - val extension - - output: - tuple val(meta), path("*.${extension}"), emit: genomecov - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def args_list = args.tokenize() - args += (scale > 0 && scale != 1) ? " -scale $scale" : "" - if (!args_list.contains('-bg') && (scale > 0 && scale != 1)) { - args += " -bg" - } - - def prefix = task.ext.prefix ?: "${meta.id}" - if (intervals.name =~ /\.bam/) { - """ - bedtools \\ - genomecov \\ - -ibam $intervals \\ - $args \\ - > ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ - } else { - """ - bedtools \\ - genomecov \\ - -i $intervals \\ - -g $sizes \\ - $args \\ - > ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ - } - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/bedtools/genomecov/meta.yml b/modules/nf-core/bedtools/genomecov/meta.yml deleted file mode 100644 index cc32da9..0000000 --- a/modules/nf-core/bedtools/genomecov/meta.yml +++ /dev/null @@ -1,59 +0,0 @@ -name: bedtools_genomecov -description: Computes histograms (default), per-base reports (-d) and BEDGRAPH (-bg) summaries of feature coverage (e.g., aligned sequences) for a given genome. -keywords: - - bed - - bam - - genomecov - - bedtools - - histogram -tools: - - bedtools: - description: | - A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. - documentation: https://bedtools.readthedocs.io/en/latest/content/tools/genomecov.html - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - intervals: - type: file - description: BAM/BED/GFF/VCF - pattern: "*.{bam|bed|gff|vcf}" - - scale: - type: integer - description: Number containing the scale factor for the output. Set to 1 to disable. Setting to a value other than 1 will also get the -bg bedgraph output format as this is required for this command switch - - sizes: - type: file - description: Tab-delimited table of chromosome names in the first column and chromosome sizes in the second column - - extension: - type: string - description: Extension of the output file (e. g., ".bg", ".bedgraph", ".txt", ".tab", etc.) It is set arbitrarily by the user and corresponds to the file format which depends on arguments. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - genomecov: - type: file - description: Computed genome coverage file - pattern: "*.${extension}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@Emiller88" - - "@sruthipsuresh" - - "@drpatelh" - - "@sidorov-si" - - "@chris-cheshire" -maintainers: - - "@Emiller88" - - "@sruthipsuresh" - - "@drpatelh" - - "@sidorov-si" - - "@chris-cheshire" diff --git a/modules/nf-core/bedtools/intersect/environment.yml b/modules/nf-core/bedtools/intersect/environment.yml deleted file mode 100644 index f8bb5fb..0000000 --- a/modules/nf-core/bedtools/intersect/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bedtools_intersect -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bedtools=2.30.0 diff --git a/modules/nf-core/bedtools/intersect/main.nf b/modules/nf-core/bedtools/intersect/main.nf deleted file mode 100644 index 8d6f280..0000000 --- a/modules/nf-core/bedtools/intersect/main.nf +++ /dev/null @@ -1,59 +0,0 @@ -process BEDTOOLS_INTERSECT { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' : - 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" - - input: - tuple val(meta), path(intervals1), path(intervals2) - tuple val(meta2), path(chrom_sizes) - - output: - tuple val(meta), path("*.${extension}"), emit: intersect - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - //Extension of the output file. It is set by the user via "ext.suffix" in the config. Corresponds to the file format which depends on arguments (e. g., ".bed", ".bam", ".txt", etc.). - extension = task.ext.suffix ?: "${intervals1.extension}" - def sizes = chrom_sizes ? "-g ${chrom_sizes}" : '' - if ("$intervals1" == "${prefix}.${extension}" || - "$intervals2" == "${prefix}.${extension}") - error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - bedtools \\ - intersect \\ - -a $intervals1 \\ - -b $intervals2 \\ - $args \\ - $sizes \\ - > ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - extension = task.ext.suffix ?: "bed" - if ("$intervals1" == "${prefix}.${extension}" || - "$intervals2" == "${prefix}.${extension}") - error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - touch ${prefix}.${extension} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/bedtools/intersect/meta.yml b/modules/nf-core/bedtools/intersect/meta.yml deleted file mode 100644 index 8d9a08a..0000000 --- a/modules/nf-core/bedtools/intersect/meta.yml +++ /dev/null @@ -1,59 +0,0 @@ -name: bedtools_intersect -description: Allows one to screen for overlaps between two sets of genomic features. -keywords: - - bed - - intersect - - overlap -tools: - - bedtools: - description: | - A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. - documentation: https://bedtools.readthedocs.io/en/latest/content/tools/intersect.html - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - intervals1: - type: file - description: BAM/BED/GFF/VCF - pattern: "*.{bam|bed|gff|vcf}" - - intervals2: - type: file - description: BAM/BED/GFF/VCF - pattern: "*.{bam|bed|gff|vcf}" - - meta2: - type: map - description: | - Groovy Map containing reference chromosome sizes - e.g. [ id:'test' ] - - chrom_sizes: - type: file - description: Chromosome sizes file - pattern: "*{.sizes,.txt}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - intersect: - type: file - description: File containing the description of overlaps found between the two features - pattern: "*.${extension}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@Emiller88" - - "@sruthipsuresh" - - "@drpatelh" - - "@sidorov-si" -maintainers: - - "@Emiller88" - - "@sruthipsuresh" - - "@drpatelh" - - "@sidorov-si" diff --git a/modules/nf-core/bedtools/merge/environment.yml b/modules/nf-core/bedtools/merge/environment.yml deleted file mode 100644 index 961e602..0000000 --- a/modules/nf-core/bedtools/merge/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bedtools_merge -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bedtools=2.31.0 diff --git a/modules/nf-core/bedtools/merge/main.nf b/modules/nf-core/bedtools/merge/main.nf deleted file mode 100644 index 153145b..0000000 --- a/modules/nf-core/bedtools/merge/main.nf +++ /dev/null @@ -1,47 +0,0 @@ -process BEDTOOLS_MERGE { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' : - 'quay.io/biocontainers/bedtools:2.31.0--hf5e1c6e_2' }" - - input: - tuple val(meta), path(bed) - - output: - tuple val(meta), path('*.bed'), emit: bed - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - if ("$bed" == "${prefix}.bed") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - bedtools \\ - merge \\ - -i $bed \\ - $args \\ - > ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/bedtools/merge/meta.yml b/modules/nf-core/bedtools/merge/meta.yml deleted file mode 100644 index 5565ce4..0000000 --- a/modules/nf-core/bedtools/merge/meta.yml +++ /dev/null @@ -1,45 +0,0 @@ -name: bedtools_merge -description: combines overlapping or “book-ended” features in an interval file into a single feature which spans all of the combined features. -keywords: - - bed - - merge - - bedtools - - overlapped bed -tools: - - bedtools: - description: | - A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. - documentation: https://bedtools.readthedocs.io/en/latest/content/tools/merge.html - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bed: - type: file - description: Input BED file - pattern: "*.{bed}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bed: - type: file - description: Overlapped bed file with combined features - pattern: "*.{bed}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@Emiller88" - - "@sruthipsuresh" - - "@drpatelh" -maintainers: - - "@Emiller88" - - "@sruthipsuresh" - - "@drpatelh" diff --git a/modules/nf-core/bedtools/subtract/environment.yml b/modules/nf-core/bedtools/subtract/environment.yml deleted file mode 100644 index b869a4f..0000000 --- a/modules/nf-core/bedtools/subtract/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bedtools_subtract -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bedtools=2.30.0 diff --git a/modules/nf-core/bedtools/subtract/main.nf b/modules/nf-core/bedtools/subtract/main.nf deleted file mode 100644 index 028660d..0000000 --- a/modules/nf-core/bedtools/subtract/main.nf +++ /dev/null @@ -1,50 +0,0 @@ -process BEDTOOLS_SUBTRACT { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0' : - 'biocontainers/bedtools:2.30.0--hc088bd4_0' }" - - input: - tuple val(meta), path(intervals1), path(intervals2) - - output: - tuple val(meta), path("*.bed"), emit: bed - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - if ("$intervals1" == "${prefix}.bed" || - "$intervals2" == "${prefix}.bed") - error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" - """ - bedtools \\ - subtract \\ - -a $intervals1 \\ - -b $intervals2 \\ - $args \\ - > ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/bedtools/subtract/meta.yml b/modules/nf-core/bedtools/subtract/meta.yml deleted file mode 100644 index 0226ff1..0000000 --- a/modules/nf-core/bedtools/subtract/meta.yml +++ /dev/null @@ -1,45 +0,0 @@ -name: bedtools_subtract -description: Finds overlaps between two sets of regions (A and B), removes the overlaps from A and reports the remaining portion of A. -keywords: - - bed - - gff - - vcf - - subtract -tools: - - bedtools: - description: | - A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. - documentation: https://bedtools.readthedocs.io/en/latest/content/tools/subtract.html - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - intervals1: - type: file - description: BED/GFF/VCF - pattern: "*.{bed|gff|vcf}" - - intervals2: - type: file - description: BED/GFF/VCF - pattern: "*.{bed|gff|vcf}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bed: - type: file - description: File containing the difference between the two sets of features - patters: "*.bed" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@sidorov-si" -maintainers: - - "@sidorov-si" diff --git a/modules/nf-core/bedtools/unionbedg/environment.yml b/modules/nf-core/bedtools/unionbedg/environment.yml deleted file mode 100644 index 895a72f..0000000 --- a/modules/nf-core/bedtools/unionbedg/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bedtools_unionbedg -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bedtools=2.31.0 diff --git a/modules/nf-core/bedtools/unionbedg/main.nf b/modules/nf-core/bedtools/unionbedg/main.nf deleted file mode 100644 index 68b9054..0000000 --- a/modules/nf-core/bedtools/unionbedg/main.nf +++ /dev/null @@ -1,50 +0,0 @@ -process BEDTOOLS_UNIONBEDG { - tag "$meta.id" - label 'process_low' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bedtools:2.31.0--hf5e1c6e_2' : - 'biocontainers/bedtools:2.31.0--hf5e1c6e_2' }" - - input: - tuple val(meta), path(bedgraph) - tuple val(meta2), path(chrom_sizes) - - output: - tuple val(meta), path("*.bed"), emit: bed - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def sizes = chrom_sizes ? "-g ${chrom_sizes}" : '' - bedgraph.collect { if ("$it" == "${prefix}.bed") error "$it has the same name as the output, use \"task.ext.prefix\" to disambiguate!" } - """ - bedtools \\ - unionbedg \\ - -i $bedgraph \\ - $sizes \\ - $args \\ - > ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bedtools: \$(bedtools --version | sed -e "s/bedtools v//g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/bedtools/unionbedg/meta.yml b/modules/nf-core/bedtools/unionbedg/meta.yml deleted file mode 100644 index ee73e8b..0000000 --- a/modules/nf-core/bedtools/unionbedg/meta.yml +++ /dev/null @@ -1,52 +0,0 @@ -name: bedtools_unionbedg -description: Combines multiple BedGraph files into a single file -keywords: - - bed - - unionBedGraphs - - bedGraph - - comparisons - - combine -tools: - - bedtools: - description: | - A set of tools for genomic analysis tasks, specifically enabling genome arithmetic (merge, count, complement) on various file types. - documentation: https://bedtools.readthedocs.io/en/latest/content/tools/slop.html - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bedgraph: - type: file - description: | - Input BedGraph file: four column BED format, with 4th column with numerical values: integer or real, positive or negative - pattern: "*.{bedGraph,bedgraph}" - - meta2: - type: map - description: | - Groovy Map containing meta information for the reference chromosome sizes - e.g. [ id:'test' ] - - chrom_sizes: - type: file - description: Chromosome sizes file - pattern: "*{.sizes,.txt}" -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - bed: - type: file - description: Combined BED file with values from all bedGraph files - pattern: "*.{bed}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@ekushele" -maintainers: - - "@ekushele" diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml deleted file mode 100644 index 1787b38..0000000 --- a/modules/nf-core/fastqc/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: fastqc -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf deleted file mode 100644 index 9e19a74..0000000 --- a/modules/nf-core/fastqc/main.nf +++ /dev/null @@ -1,55 +0,0 @@ -process FASTQC { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : - 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" - - input: - tuple val(meta), path(reads) - - output: - tuple val(meta), path("*.html"), emit: html - tuple val(meta), path("*.zip") , emit: zip - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - // Make list of old name and new name pairs to use for renaming in the bash while loop - def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do - [ -f "\${new_name}" ] || ln -s \$old_name \$new_name - done - - fastqc \\ - $args \\ - --threads $task.cpus \\ - $renamed_files - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - """ - touch ${prefix}.html - touch ${prefix}.zip - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml deleted file mode 100644 index ee5507e..0000000 --- a/modules/nf-core/fastqc/meta.yml +++ /dev/null @@ -1,57 +0,0 @@ -name: fastqc -description: Run FastQC on sequenced reads -keywords: - - quality control - - qc - - adapters - - fastq -tools: - - fastqc: - description: | - FastQC gives general quality metrics about your reads. - It provides information about the quality score distribution - across your reads, the per base sequence content (%A/C/G/T). - You get information about adapter contamination and other - overrepresented sequences. - homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ - documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ - licence: ["GPL-2.0-only"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" - - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" -maintainers: - - "@drpatelh" - - "@grst" - - "@ewels" - - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test deleted file mode 100644 index 70edae4..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ /dev/null @@ -1,212 +0,0 @@ -nextflow_process { - - name "Test Process FASTQC" - script "../main.nf" - process "FASTQC" - - tag "modules" - tag "modules_nfcore" - tag "fastqc" - - test("sarscov2 single-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
    Mon 2 Oct 2023
    test.gz
    - // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("    ") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_single") } - ) - } - } - - test("sarscov2 paired-end [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_paired") } - ) - } - } - - test("sarscov2 interleaved [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } - ) - } - } - - test("sarscov2 paired-end [bam]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_bam") } - ) - } - } - - test("sarscov2 multiple [fastq]") { - - when { - process { - """ - input[0] = Channel.of([ - [id: 'test', single_end: false], // meta map - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("") }, - { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert path(process.out.html[0][1][2]).text.contains("") }, - { assert path(process.out.html[0][1][3]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } - ) - } - } - - test("sarscov2 custom_prefix") { - - when { - process { - """ - input[0] = Channel.of([ - [ id:'mysample', single_end:true ], // meta map - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } - ) - } - } - - test("sarscov2 single-end [fastq] - stub") { - - options "-stub" - - when { - process { - """ - input[0] = Channel.of([ - [ id: 'test', single_end:true ], - [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] - ]) - """ - } - } - - then { - assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match("fastqc_stub") } - ) - } - } - -} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap deleted file mode 100644 index 86f7c31..0000000 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ /dev/null @@ -1,88 +0,0 @@ -{ - "fastqc_versions_interleaved": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:07.293713" - }, - "fastqc_stub": { - "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:31:01.425198" - }, - "fastqc_versions_multiple": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:55.797907" - }, - "fastqc_versions_bam": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:40:26.795862" - }, - "fastqc_versions_single": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:27.043675" - }, - "fastqc_versions_paired": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:39:47.584191" - }, - "fastqc_versions_custom_prefix": { - "content": [ - [ - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-01-31T17:41:14.576531" - } -} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294..0000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/manta/convertinversion/environment.yml b/modules/nf-core/manta/convertinversion/environment.yml new file mode 100644 index 0000000..c238cf4 --- /dev/null +++ b/modules/nf-core/manta/convertinversion/environment.yml @@ -0,0 +1,9 @@ +name: manta_convertinversion +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::manta=1.6.0 + - bioconda::samtools=1.16.1 + - bioconda::htslib=1.16 diff --git a/modules/nf-core/manta/convertinversion/main.nf b/modules/nf-core/manta/convertinversion/main.nf index f771b74..dde263d 100644 --- a/modules/nf-core/manta/convertinversion/main.nf +++ b/modules/nf-core/manta/convertinversion/main.nf @@ -1,20 +1,21 @@ process MANTA_CONVERTINVERSION { tag "$meta.id" - label 'process_single' + label 'process_low' label 'error_retry' - conda "bioconda::manta=1.6.0 bioconda::samtools=1.16.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-40295ae41112676b05b649e513fe7000675e9b84:a0332aa38645fbb8969567731ce68cfb7f830ec4-0': - 'quay.io/biocontainers/mulled-v2-40295ae41112676b05b649e513fe7000675e9b84:a0332aa38645fbb8969567731ce68cfb7f830ec4-0' }" + 'biocontainers/mulled-v2-40295ae41112676b05b649e513fe7000675e9b84:a0332aa38645fbb8969567731ce68cfb7f830ec4-0' }" input: - tuple val(meta), path(vcf), path(index) - tuple path(fasta), path(fai) + tuple val(meta), path(vcf) + tuple val(meta2), path(fasta) output: - tuple val(meta), path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf_tabi - path "versions.yml" , emit: versions + tuple val(meta), path("*.vcf.gz") , emit: vcf + tuple val(meta), path("*.vcf.gz.tbi"), emit: tbi + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -23,14 +24,13 @@ process MANTA_CONVERTINVERSION { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ - convertInversion.py \$(which samtools) $fasta $vcf | bgzip --threads $task.cpus > ${prefix}.converted.vcf.gz - tabix ${prefix}.converted.vcf.gz + convertInversion.py \$(which samtools) $fasta $vcf | bgzip --threads $task.cpus > ${prefix}.vcf.gz + tabix ${prefix}.vcf.gz cat <<-END_VERSIONS > versions.yml "${task.process}": manta: \$( configManta.py --version ) - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' )) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' ) END_VERSIONS """ - } diff --git a/modules/nf-core/manta/convertinversion/meta.yml b/modules/nf-core/manta/convertinversion/meta.yml index cf9e169..cd1d3ce 100644 --- a/modules/nf-core/manta/convertinversion/meta.yml +++ b/modules/nf-core/manta/convertinversion/meta.yml @@ -12,7 +12,6 @@ tools: tool_dev_url: https://github.com/Illumina/manta doi: "10.1093/bioinformatics/btv710" licence: ["GPL v3"] - input: - meta: type: map @@ -23,7 +22,6 @@ input: type: file description: VCF file produces by Manta pattern: "*.vcf.gz" - output: - meta: type: map @@ -44,3 +42,5 @@ output: pattern: "*.vcf.gz.tbi" authors: - "@FriederikeHanssen" +maintainers: + - "@FriederikeHanssen" diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index b866174..ca39fb6 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -1,7 +1,7 @@ -name: MultiQC +name: multiqc channels: - conda-forge - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index b4bb4bf..47ac352 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml @@ -41,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index a61223e..45a9bc3 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b48..bfebd80 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/modules/nf-core/survivor/filter/main.nf b/modules/nf-core/survivor/filter/main.nf index a85bf62..d14ba0a 100644 --- a/modules/nf-core/survivor/filter/main.nf +++ b/modules/nf-core/survivor/filter/main.nf @@ -5,7 +5,7 @@ process SURVIVOR_FILTER { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/survivor:1.0.7--h9a82719_1': - 'quay.io/biocontainers/survivor:1.0.7--h9a82719_1' }" + 'biocontainers/survivor:1.0.7--h9a82719_1' }" input: tuple val(meta),path(vcf_file), path(bed) // VCF file to filter and BED file with regions to ignore (NA to disable) diff --git a/modules/nf-core/survivor/stats/main.nf b/modules/nf-core/survivor/stats/main.nf index 1b41304..906536d 100644 --- a/modules/nf-core/survivor/stats/main.nf +++ b/modules/nf-core/survivor/stats/main.nf @@ -5,36 +5,42 @@ process SURVIVOR_STATS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/survivor:1.0.7--h9a82719_1': - 'quay.io/biocontainers/survivor:1.0.7--h9a82719_1' }" + 'biocontainers/survivor:1.0.7--h9a82719_1' }" input: - tuple val(meta), path(vcf), path(index) + tuple val(meta), path(vcf) val(minsv) // Min SV size (-1 to disable) val(maxsv) // Max SV size (-1 to disable) val(minnumreads) // Min number of reads support: RE flag (-1 to disable) output: - tuple val(meta),path("*.stats"), emit: stats + tuple val(meta), path("*.stats"), emit: stats path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def name = vcf.getBaseName() - + def is_compressed = vcf.getName().endsWith(".gz") ? true : false + vcf_name = vcf.getName().replace(".gz", "") + """ - gzip -d $vcf + if [ "$is_compressed" == "true" ]; then + gzip -c -d $vcf > $vcf_name + fi SURVIVOR \\ stats \\ - $name \\ + $vcf_name \\ $minsv \\ $maxsv \\ $minnumreads \\ ${prefix}.stats + rm $vcf_name + cat <<-END_VERSIONS > versions.yml "${task.process}": survivor: \$(echo \$(SURVIVOR 2>&1 | grep "Version" | sed 's/^Version: //')) diff --git a/modules/nf-core/survivor/stats/tests/main.nf.test.snap b/modules/nf-core/survivor/stats/tests/main.nf.test.snap index d629dfc..723a1bd 100644 --- a/modules/nf-core/survivor/stats/tests/main.nf.test.snap +++ b/modules/nf-core/survivor/stats/tests/main.nf.test.snap @@ -6,14 +6,14 @@ { "id": "test" }, - "test.stats:md5,023a37c7d6fab374c9bdcf98c011022c" + "test.stats:md5,d51fb5cc28255f37096cd0f3a6de5f8d" ] ], [ "versions.yml:md5,35bf44775e74ffec88173cd2093c2928" ] ], - "timestamp": "2024-01-15T15:37:42.562568997" + "timestamp": "2024-01-24T14:37:15.916803283" }, "homo_sapiens - mutect - stub": { "content": [ @@ -29,6 +29,6 @@ "versions.yml:md5,35bf44775e74ffec88173cd2093c2928" ] ], - "timestamp": "2024-01-15T15:27:09.132671858" + "timestamp": "2024-01-24T14:37:26.628898765" } } \ No newline at end of file diff --git a/modules/nf-core/svanalyzer/svbenchmark/main.nf b/modules/nf-core/svanalyzer/svbenchmark/main.nf index 949e2cc..5d2d079 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/main.nf +++ b/modules/nf-core/svanalyzer/svbenchmark/main.nf @@ -6,7 +6,7 @@ process SVANALYZER_SVBENCHMARK { conda "bioconda::svanalyzer=0.35" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/svanalyzer:0.35--pl526_0': - 'quay.io/biocontainers/svanalyzer:0.35--pl526_0' }" + 'biocontainers/svanalyzer:0.35--pl526_0' }" input: tuple val(meta),path(test), path(test_index), path(truth), path(truth_index), path(bed) diff --git a/modules/nf-core/svync/main.nf b/modules/nf-core/svync/main.nf index 8c82a92..fe9c28c 100644 --- a/modules/nf-core/svync/main.nf +++ b/modules/nf-core/svync/main.nf @@ -5,14 +5,14 @@ process SVYNC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/svync:0.1.2--h9ee0642_0': - 'quay.io/biocontainers/svync:0.1.2--h9ee0642_0' }" + 'biocontainers/svync:0.1.2--h9ee0642_0' }" input: - tuple val(meta),path(vcf), path(tbi), path(config) + tuple val(meta), path(vcf), path(tbi), path(config) output: - tuple val(meta),path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.vcf.gz"), emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -31,7 +31,20 @@ process SVYNC { --input $vcf \\ | bgzip --threads $task.cpus $args2 > ${prefix}.vcf.gz - tabix ${prefix}.vcf.gz + cat <<-END_VERSIONS > versions.yml + "${task.process}": + svync: \$(svync --version | sed 's/svync version //') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + if ("$vcf" == "${prefix}.vcf.gz") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + + """ + echo | gzip -n > ${prefix}.vcf.gz + cat <<-END_VERSIONS > versions.yml "${task.process}": svync: \$(svync --version | sed 's/svync version //') diff --git a/modules/nf-core/svync/meta.yml b/modules/nf-core/svync/meta.yml index 3846ae2..dacc573 100644 --- a/modules/nf-core/svync/meta.yml +++ b/modules/nf-core/svync/meta.yml @@ -29,10 +29,6 @@ input: type: file description: The index of the input VCF file containing structural variants pattern: "*.tbi" - - meta2: - type: map - description: | - Groovy Map containing config meta information - config: type: file description: The config stating how the standardization should happen diff --git a/modules/nf-core/svync/tests/main.nf.test b/modules/nf-core/svync/tests/main.nf.test index cc618f9..0be4926 100644 --- a/modules/nf-core/svync/tests/main.nf.test +++ b/modules/nf-core/svync/tests/main.nf.test @@ -13,19 +13,19 @@ nextflow_process { when { process { """ - input[0] = [ + vcf = Channel.of([ [ id:'test', single_end:false ], // meta map file(params.test_data['homo_sapiens']['illumina']['simulated_sv'], checkIfExists: true), file(params.test_data['homo_sapiens']['illumina']['simulated_sv_tbi'], checkIfExists: true) - ] - - input[1] = Channel.of([ - "config.yaml", - "info:", - " SVMETHOD:", - " value: svync", - " description: Type of approach used to detect SV", - " number: 1", + ]) + + config = Channel.of([ + "config.yaml", + "info:", + " SVMETHOD:", + " value: svync", + " description: Type of approach used to detect SV", + " number: 1", " type: string", "format:", " GT:", @@ -35,7 +35,9 @@ nextflow_process { " type: string" ]) .collectFile(newLine:true, sort:'index') - .map { [[], it] } + .map { [[ id:'test', single_end:false ], it] } + + input[0] = vcf.join(config) """ } } @@ -59,15 +61,18 @@ nextflow_process { when { process { """ - input[0] = [ + vcf = Channel.of([ [ id:'test', single_end:false ], // meta map file(params.test_data['homo_sapiens']['illumina']['simulated_sv'], checkIfExists: true), file(params.test_data['homo_sapiens']['illumina']['simulated_sv_tbi'], checkIfExists: true) - ] + ]) - input[1] = Channel.of(["config.yaml", "info:", " SVMETHOD:", " value: svync", " description: Type of approach used to detect SV", " number: 1", " type: string"]) + config = Channel.of(["config.yaml", "info:", " SVMETHOD:", " value: svync", " description: Type of approach used to detect SV", " number: 1", " type: string"]) .collectFile(newLine:true, sort:'index') - .map { [[], it] } + .map { [[ id:'test', single_end:false ], it] } + + input[0] = vcf.join(config) + """ } } diff --git a/modules/nf-core/tabix/bgzip/environment.yml b/modules/nf-core/tabix/bgzip/environment.yml index 4fe40c5..361c078 100644 --- a/modules/nf-core/tabix/bgzip/environment.yml +++ b/modules/nf-core/tabix/bgzip/environment.yml @@ -5,3 +5,4 @@ channels: - defaults dependencies: - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/tabix/bgzip/main.nf b/modules/nf-core/tabix/bgzip/main.nf index 346c1ba..3065dab 100644 --- a/modules/nf-core/tabix/bgzip/main.nf +++ b/modules/nf-core/tabix/bgzip/main.nf @@ -4,15 +4,15 @@ process TABIX_BGZIP { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/htslib:1.19.1--h81da01d_1' : + 'biocontainers/htslib:1.19.1--h81da01d_1' }" input: - tuple val(meta),path(input) + tuple val(meta), path(input) output: - tuple val(meta),path("${output}") , emit: output - tuple val(meta),path("${output}.gzi"), emit: gzi, optional: true + tuple val(meta), path("${output}") , emit: output + tuple val(meta), path("${output}.gzi"), emit: gzi, optional: true path "versions.yml" , emit: versions when: @@ -44,7 +44,8 @@ process TABIX_BGZIP { output = in_bgzip ? input.getBaseName() : "${prefix}.${input.getExtension()}.gz" """ - touch ${output} + echo "" | gzip > ${output} + touch ${output}.gzi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config b/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config new file mode 100644 index 0000000..6b6ff55 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/bgzip_compress.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIP { + ext.args = ' -i' + } +} diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test b/modules/nf-core/tabix/bgzip/tests/main.nf.test new file mode 100644 index 0000000..95fd4c5 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/main.nf.test @@ -0,0 +1,111 @@ +nextflow_process { + + name "Test Process TABIX_BGZIP" + script "modules/nf-core/tabix/bgzip/main.nf" + process "TABIX_BGZIP" + + tag "modules" + tag "modules_nfcore" + tag "tabix" + tag "tabix/bgzip" + + test("sarscov2_vcf_bgzip_compress") { + when { + process { + """ + input[0] = [ + [ id:'bgzip_test' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.output[0][1]).name + ).match("bgzip_test") + } + ) + } + } + + test("homo_genome_bedgz_compress") { + when { + process { + """ + input[0] = [ + [ id:'bedgz_test' ], + [ file(params.test_data['homo_sapiens']['genome']['genome_bed_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.output[0][1]).name + ).match("bedgz_test") + } + ) + } + } + + test("sarscov2_vcf_bgzip_compress_stub") { + options '-stub' + config "./bgzip_compress.config" + + when { + process { + """ + input[0] = [ + [ id:"test_stub" ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.output[0][1]).name + ).match("test_stub") + } + ) + } + } + + test("sarscov2_vcf_bgzip_compress_gzi") { + config "./bgzip_compress.config" + when { + process { + """ + input[0] = [ + [ id:"gzi_compress_test" ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gzi[0][1]).name + ).match("gzi_compress_test") + } + ) + } + } +} diff --git a/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap b/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap new file mode 100644 index 0000000..53d5993 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/main.nf.test.snap @@ -0,0 +1,186 @@ +{ + "gzi_compress_test": { + "content": [ + "gzi_compress_test.vcf.gz.gzi" + ], + "timestamp": "2024-02-19T14:52:29.328146" + }, + "homo_genome_bedgz_compress": { + "content": [ + { + "0": [ + [ + { + "id": "bedgz_test" + }, + "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + + ], + "output": [ + [ + { + "id": "bedgz_test" + }, + "bedgz_test.bed:md5,87a15eb9c2ff20ccd5cd8735a28708f7" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:12.422209" + }, + "test_stub": { + "content": [ + "test_stub.vcf.gz" + ], + "timestamp": "2024-02-19T14:52:20.811489" + }, + "sarscov2_vcf_bgzip_compress": { + "content": [ + { + "0": [ + [ + { + "id": "bgzip_test" + }, + "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + + ], + "output": [ + [ + { + "id": "bgzip_test" + }, + "bgzip_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:03.706028" + }, + "sarscov2_vcf_bgzip_compress_gzi": { + "content": [ + { + "0": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0" + ] + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz.gzi:md5,26fd00d4e26141cd11561f6e7d4a2ad0" + ] + ], + "output": [ + [ + { + "id": "gzi_compress_test" + }, + "gzi_compress_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:29.271494" + }, + "bgzip_test": { + "content": [ + "bgzip_test.vcf.gz" + ], + "timestamp": "2024-02-19T14:52:03.768295" + }, + "bedgz_test": { + "content": [ + "bedgz_test.bed" + ], + "timestamp": "2024-02-19T14:52:12.453855" + }, + "sarscov2_vcf_bgzip_compress_stub": { + "content": [ + { + "0": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ], + "gzi": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz.gzi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "output": [ + [ + { + "id": "test_stub" + }, + "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,e023292de6ee109a44fc67475d658174" + ] + } + ], + "timestamp": "2024-02-19T14:52:20.769619" + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/bgzip/tests/tags.yml b/modules/nf-core/tabix/bgzip/tests/tags.yml new file mode 100644 index 0000000..de0eec8 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/tags.yml @@ -0,0 +1,2 @@ +tabix/bgzip: + - "modules/nf-core/tabix/bgzip/**" diff --git a/modules/nf-core/tabix/bgzip/tests/vcf_none.config b/modules/nf-core/tabix/bgzip/tests/vcf_none.config new file mode 100644 index 0000000..f3a3c46 --- /dev/null +++ b/modules/nf-core/tabix/bgzip/tests/vcf_none.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIP { + ext.args = '' + } +} diff --git a/modules/nf-core/tabix/bgziptabix/environment.yml b/modules/nf-core/tabix/bgziptabix/environment.yml index 028461c..c423587 100644 --- a/modules/nf-core/tabix/bgziptabix/environment.yml +++ b/modules/nf-core/tabix/bgziptabix/environment.yml @@ -5,3 +5,4 @@ channels: - defaults dependencies: - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/tabix/bgziptabix/main.nf b/modules/nf-core/tabix/bgziptabix/main.nf index 07f442d..bcdcf2a 100644 --- a/modules/nf-core/tabix/bgziptabix/main.nf +++ b/modules/nf-core/tabix/bgziptabix/main.nf @@ -4,15 +4,15 @@ process TABIX_BGZIPTABIX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/htslib:1.19.1--h81da01d_1' : + 'biocontainers/htslib:1.19.1--h81da01d_1' }" input: - tuple val(meta),path(input) + tuple val(meta), path(input) output: - tuple val(meta),path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi - tuple val(meta),path("*.gz"), path("*.csi"), optional: true, emit: gz_csi + tuple val(meta), path("*.gz"), path("*.tbi"), optional: true, emit: gz_tbi + tuple val(meta), path("*.gz"), path("*.csi"), optional: true, emit: gz_csi path "versions.yml" , emit: versions when: @@ -35,7 +35,7 @@ process TABIX_BGZIPTABIX { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.${input.getExtension()}.gz + echo "" | gzip > ${prefix}.${input.getExtension()}.gz touch ${prefix}.${input.getExtension()}.gz.tbi touch ${prefix}.${input.getExtension()}.gz.csi diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test new file mode 100644 index 0000000..87ea2c8 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test @@ -0,0 +1,94 @@ +nextflow_process { + + name "Test Process TABIX_BGZIPTABIX" + script "modules/nf-core/tabix/bgziptabix/main.nf" + process "TABIX_BGZIPTABIX" + + tag "modules" + tag "modules_nfcore" + tag "tabix" + tag "tabix/bgziptabix" + + test("sarscov2_bed_tbi") { + config "./tabix_tbi.config" + + when { + process { + """ + input[0] = [ + [ id:'tbi_test' ], + [ file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gz_tbi[0][1]).name + ).match("tbi_test") + } + ) + } + } + + test("sarscov2_bed_csi") { + config "./tabix_csi.config" + + when { + process { + """ + input[0] = [ + [ id:'csi_test' ], + [ file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gz_csi[0][1]).name + ).match("csi_test") + } + ) + } + + } + + test("sarscov2_bed_csi_stub") { + config "./tabix_csi.config" + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], + [ file(params.test_data['sarscov2']['genome']['test_bed'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.gz_csi[0][1]).name + ).match("csi_stub") + } + ) + } + + } + +} diff --git a/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap new file mode 100644 index 0000000..fcecb2e --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/main.nf.test.snap @@ -0,0 +1,143 @@ +{ + "sarscov2_bed_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_test" + }, + "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "tbi_test.bed.gz.tbi:md5,24908545311cf2b7c803c41d716872c4" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ], + "gz_csi": [ + + ], + "gz_tbi": [ + [ + { + "id": "tbi_test" + }, + "tbi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "tbi_test.bed.gz.tbi:md5,24908545311cf2b7c803c41d716872c4" + ] + ], + "versions": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ] + } + ], + "timestamp": "2024-02-19T14:50:51.513838" + }, + "sarscov2_bed_csi": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "csi_test" + }, + "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "csi_test.bed.gz.csi:md5,e06165ddd34640783728cb07f2558b43" + ] + ], + "2": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ], + "gz_csi": [ + [ + { + "id": "csi_test" + }, + "csi_test.bed.gz:md5,fe4053cf4de3aebbdfc3be2efb125a74", + "csi_test.bed.gz.csi:md5,e06165ddd34640783728cb07f2558b43" + ] + ], + "gz_tbi": [ + + ], + "versions": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ] + } + ], + "timestamp": "2024-02-19T14:51:00.513777" + }, + "csi_test": { + "content": [ + "csi_test.bed.gz" + ], + "timestamp": "2024-02-19T14:51:00.548801" + }, + "csi_stub": { + "content": [ + "test.bed.gz" + ], + "timestamp": "2024-02-19T14:51:09.218454" + }, + "tbi_test": { + "content": [ + "tbi_test.bed.gz" + ], + "timestamp": "2024-02-19T14:50:51.579654" + }, + "sarscov2_bed_csi_stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ], + "gz_csi": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "gz_tbi": [ + [ + { + "id": "test" + }, + "test.bed.gz:md5,68b329da9893e34099c7d8ad5cb9c940", + "test.bed.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,b4765e4d896ce4a4cdd6c896d12555fc" + ] + } + ], + "timestamp": "2024-02-19T14:51:09.164254" + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config new file mode 100644 index 0000000..fb41a31 --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/tabix_csi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIPTABIX { + ext.args2 = '-p vcf --csi' + } +} diff --git a/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config b/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config new file mode 100644 index 0000000..c1915dc --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/tabix_tbi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_BGZIPTABIX { + ext.args2 = '-p vcf' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/bgziptabix/tests/tags.yml b/modules/nf-core/tabix/bgziptabix/tests/tags.yml new file mode 100644 index 0000000..5052b4d --- /dev/null +++ b/modules/nf-core/tabix/bgziptabix/tests/tags.yml @@ -0,0 +1,2 @@ +tabix/bgziptabix: + - "modules/nf-core/tabix/bgziptabix/**" diff --git a/modules/nf-core/tabix/tabix/environment.yml b/modules/nf-core/tabix/tabix/environment.yml index 7167fb8..76b45e1 100644 --- a/modules/nf-core/tabix/tabix/environment.yml +++ b/modules/nf-core/tabix/tabix/environment.yml @@ -5,3 +5,4 @@ channels: - defaults dependencies: - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/tabix/tabix/main.nf b/modules/nf-core/tabix/tabix/main.nf index 58dbd83..f5e582d 100644 --- a/modules/nf-core/tabix/tabix/main.nf +++ b/modules/nf-core/tabix/tabix/main.nf @@ -4,15 +4,15 @@ process TABIX_TABIX { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'quay.io/biocontainers/tabix:1.11--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/htslib:1.19.1--h81da01d_1' : + 'biocontainers/htslib:1.19.1--h81da01d_1' }" input: - tuple val(meta),path(tab) + tuple val(meta), path(tab) output: - tuple val(meta),path("*.tbi"), optional:true, emit: tbi - tuple val(meta),path("*.csi"), optional:true, emit: csi + tuple val(meta), path("*.tbi"), optional:true, emit: tbi + tuple val(meta), path("*.csi"), optional:true, emit: csi path "versions.yml" , emit: versions when: @@ -32,7 +32,8 @@ process TABIX_TABIX { stub: def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${tab}.tbi + touch ${prefix}.tbi + touch ${prefix}.csi cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test b/modules/nf-core/tabix/tabix/tests/main.nf.test new file mode 100644 index 0000000..3a150c7 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/main.nf.test @@ -0,0 +1,142 @@ +nextflow_process { + + name "Test Process TABIX_TABIX" + script "modules/nf-core/tabix/tabix/main.nf" + process "TABIX_TABIX" + + tag "modules" + tag "modules_nfcore" + tag "tabix" + tag "tabix/tabix" + + test("sarscov2_bedgz_tbi") { + config "./tabix_bed.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_bed' ], + [ file(params.test_data['sarscov2']['genome']['test_bed_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_bed") + } + ) + } + } + + test("sarscov2_gff_tbi") { + config "./tabix_gff.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_gff' ], + [ file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_gff") + } + ) + } + + } + + test("sarscov2_vcf_tbi") { + config "./tabix_vcf_tbi.config" + when { + process { + """ + input[0] = [ + [ id:'tbi_vcf' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.tbi[0][1]).name + ).match("tbi_vcf") + } + ) + } + + } + + test("sarscov2_vcf_csi") { + config "./tabix_vcf_csi.config" + when { + process { + """ + input[0] = [ + [ id:'vcf_csi' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.csi[0][1]).name + ).match("vcf_csi") + } + ) + } + + } + + test("sarscov2_vcf_csi_stub") { + config "./tabix_vcf_csi.config" + options "-stub" + when { + process { + """ + input[0] = [ + [ id:'vcf_csi_stub' ], + [ file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot( + file(process.out.csi[0][1]).name + ).match("vcf_csi_stub") + } + ) + } + + } + +} diff --git a/modules/nf-core/tabix/tabix/tests/main.nf.test.snap b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap new file mode 100644 index 0000000..484ebe2 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/main.nf.test.snap @@ -0,0 +1,217 @@ +{ + "vcf_csi_stub": { + "content": [ + "vcf_csi_stub.csi" + ], + "timestamp": "2024-02-19T14:54:03.35052" + }, + "tbi_gff": { + "content": [ + "genome.gff3.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:37.420216" + }, + "sarscov2_gff_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_gff" + }, + "genome.gff3.gz.tbi:md5,53fc683fd217aae47ef10d23c52a9178" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_gff" + }, + "genome.gff3.gz.tbi:md5,53fc683fd217aae47ef10d23c52a9178" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:37.388157" + }, + "sarscov2_bedgz_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_bed" + }, + "test.bed.gz.tbi:md5,0f17d85e7f0a042b2aa367b70df224f8" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_bed" + }, + "test.bed.gz.tbi:md5,0f17d85e7f0a042b2aa367b70df224f8" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:28.879408" + }, + "tbi_vcf": { + "content": [ + "test.vcf.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:46.402522" + }, + "vcf_csi": { + "content": [ + "test.vcf.gz.csi" + ], + "timestamp": "2024-02-19T14:53:54.921189" + }, + "sarscov2_vcf_tbi": { + "content": [ + { + "0": [ + [ + { + "id": "tbi_vcf" + }, + "test.vcf.gz.tbi:md5,897f3f378a811b90e6dee56ce08d2bcf" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "tbi_vcf" + }, + "test.vcf.gz.tbi:md5,897f3f378a811b90e6dee56ce08d2bcf" + ] + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:46.370358" + }, + "sarscov2_vcf_csi_stub": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,3d45df6d80883bad358631069a2940fd" + ], + "csi": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + [ + { + "id": "vcf_csi_stub" + }, + "vcf_csi_stub.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,3d45df6d80883bad358631069a2940fd" + ] + } + ], + "timestamp": "2024-02-19T14:54:03.310028" + }, + "sarscov2_vcf_csi": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "vcf_csi" + }, + "test.vcf.gz.csi:md5,0731ad6f40104d2bbb1a2cc478ef8f03" + ] + ], + "2": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ], + "csi": [ + [ + { + "id": "vcf_csi" + }, + "test.vcf.gz.csi:md5,0731ad6f40104d2bbb1a2cc478ef8f03" + ] + ], + "tbi": [ + + ], + "versions": [ + "versions.yml:md5,f4feeda7fdd4b567102f7f8e5d7037a3" + ] + } + ], + "timestamp": "2024-02-19T14:53:54.886876" + }, + "tbi_bed": { + "content": [ + "test.bed.gz.tbi" + ], + "timestamp": "2024-02-19T14:53:28.947628" + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_bed.config b/modules/nf-core/tabix/tabix/tests/tabix_bed.config new file mode 100644 index 0000000..7ff0590 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_bed.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p bed' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_gff.config b/modules/nf-core/tabix/tabix/tests/tabix_gff.config new file mode 100644 index 0000000..20c0a1e --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_gff.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p gff' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config new file mode 100644 index 0000000..eb4f2d7 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_csi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p vcf --csi' + } +} diff --git a/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config new file mode 100644 index 0000000..2774c8a --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tabix_vcf_tbi.config @@ -0,0 +1,5 @@ +process { + withName: TABIX_TABIX { + ext.args = '-p vcf' + } +} \ No newline at end of file diff --git a/modules/nf-core/tabix/tabix/tests/tags.yml b/modules/nf-core/tabix/tabix/tests/tags.yml new file mode 100644 index 0000000..6eda065 --- /dev/null +++ b/modules/nf-core/tabix/tabix/tests/tags.yml @@ -0,0 +1,2 @@ +tabix/tabix: + - "modules/nf-core/tabix/tabix/**" diff --git a/nextflow.config b/nextflow.config index 5cfc390..66932f2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -33,13 +33,13 @@ params { min_sv_size = 50 // Truvari params - dup_to_ins = "" // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 + dup_to_ins = false // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 // Manta params - bnd_to_inv = "" // manta reports INV as BND + bnd_to_inv = false // manta reports INV as BND // Gridss params - gridss_annotate = "" + gridss_annotate = false // References @@ -207,10 +207,10 @@ profiles { // Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile // Will not be used unless Apptainer / Docker / Podman / Singularity are enabled // Set to your registry if you have a mirror of containers -apptainer.registry = '' -docker.registry = '' -podman.registry = '' -singularity.registry = '' +apptainer.registry = 'quay.io' +docker.registry = 'quay.io' +podman.registry = 'quay.io' +singularity.registry = 'quay.io' // Nextflow plugins plugins { diff --git a/nextflow_schema.json b/nextflow_schema.json index 12700cb..ce6d39b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -84,19 +84,19 @@ "fa_icon": "fas fa-folder-open" }, "dup_to_ins": { - "type": "string", + "type": "boolean", "format": "directory-path", "description": "", "fa_icon": "fas fa-folder-open" }, "bnd_to_inv": { - "type": "string", + "type": "boolean", "format": "directory-path", "description": "", "fa_icon": "fas fa-folder-open" }, "gridss_annotate": { - "type": "string", + "type": "boolean", "format": "directory-path", "description": "", "fa_icon": "fas fa-folder-open" diff --git a/null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt b/null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt deleted file mode 100644 index 6b739ac..0000000 --- a/null/pipeline_info/execution_trace_2024-02-23_11-23-40.txt +++ /dev/null @@ -1 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt b/null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt deleted file mode 100644 index 6b739ac..0000000 --- a/null/pipeline_info/execution_trace_2024-02-23_11-25-34.txt +++ /dev/null @@ -1 +0,0 @@ -task_id hash native_id name status exit submit duration realtime %cpu peak_rss peak_vmem rchar wchar diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 5998a65..ad04130 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -44,9 +44,16 @@ workflow PREPARE_VCFS_TEST { // PREPARE_VCFS // // Reheader needed to standardize sample names + ch_samples = Channel.of(["samples.txt", params.sample,"_query"]) + .collectFile(newLine:false) + + vcf_ch.combine(ch_samples) + .map{it -> tuple( it[0], it[1],[],it[3])} + .set{input_ch} + BCFTOOLS_REHEADER_TEST( - vcf_ch, - ref + input_ch, + ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } ) versions = versions.mix(BCFTOOLS_REHEADER_TEST.out.versions) @@ -76,8 +83,7 @@ workflow PREPARE_VCFS_TEST { // Breaks down -any- multi-allelic variants BCFTOOLS_NORM_1( vcf_ch, - ref, - [[],[]] + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } ) versions = versions.mix(BCFTOOLS_NORM_1.out.versions) @@ -121,8 +127,7 @@ workflow PREPARE_VCFS_TEST { // Deduplicates variants at the same position test BCFTOOLS_NORM_2( vcf_ch, - ref, - [[],[]] + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } ) versions = versions.mix(BCFTOOLS_NORM_2.out.versions) diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 8396ab8..87e2489 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -36,10 +36,17 @@ workflow PREPARE_VCFS_TRUTH { versions = versions.mix(BGZIP_TABIX.out.versions) vcf_ch = BGZIP_TABIX.out.gz_tbi - // Reheader needed to standardize sample names + // Reheader needed to standardize sample names + ch_samples = Channel.of(["samples.txt", params.sample,"_truth"]) + .collectFile(newLine:false) + + vcf_ch.combine(ch_samples) + .map{it -> tuple( it[0], it[1],[],it[3])} + .set{input_ch} + BCFTOOLS_REHEADER_TRUTH( - vcf_ch, - ref + input_ch, + ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } ) versions = versions.mix(BCFTOOLS_REHEADER_TRUTH.out.versions) @@ -56,8 +63,7 @@ workflow PREPARE_VCFS_TRUTH { // multi-allelic variants will be splitted. BCFTOOLS_NORM_1( vcf_ch, - ref, - [[],[]] + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } ) versions = versions.mix(BCFTOOLS_NORM_1.out.versions) @@ -76,8 +82,7 @@ workflow PREPARE_VCFS_TRUTH { // Deduplicate variants at the same position BCFTOOLS_NORM_2( vcf_ch, - ref, - [[],[]] + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } ) versions = versions.mix(BCFTOOLS_NORM_2.out.versions) diff --git a/subworkflows/local/report_vcf_statistics.nf b/subworkflows/local/report_vcf_statistics.nf index 4828408..0dcf501 100644 --- a/subworkflows/local/report_vcf_statistics.nf +++ b/subworkflows/local/report_vcf_statistics.nf @@ -24,7 +24,7 @@ workflow REPORT_VCF_STATISTICS { // SURVIVOR_STATS // SURVIVOR_STATS( - input.sv, + input.sv.map{it -> tuple( it[0], it[1])}, -1, -1, -1 diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 2ec4bef..09ab7c9 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -61,23 +61,24 @@ workflow SV_VCF_CONVERSIONS { if(params.bnd_to_inv){ out_vcf_ch = Channel.empty() - out_vcf_ch.branch{ + vcf_ch.branch{ tool: it[0].id == "manta" || it[0].id == "dragen" other: true} .set{input} // // MANTA_CONVERTINVERSION // - //NOTE: should also work for dragen + // NOTE: should also work for dragen // Not working now!!!!! MANTA_CONVERTINVERSION( - input.tool, - ref + input.tool.map{it -> tuple(it[0], it[1])}, + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } ) versions = versions.mix(MANTA_CONVERTINVERSION.out.versions) - out_vcf_ch = out_vcf_ch.mix(MANTA_CONVERTINVERSION.out.vcf_tabi) + out_ch = MANTA_CONVERTINVERSION.out.vcf.join(MANTA_CONVERTINVERSION.out.tbi) + out_vcf_ch = out_vcf_ch.mix(out_ch) out_vcf_ch = out_vcf_ch.mix(input.other) vcf_ch = out_vcf_ch @@ -88,7 +89,7 @@ workflow SV_VCF_CONVERSIONS { if (params.gridss_annotate){ out_vcf_ch = Channel.empty() - out2_vcf_ch.branch{ + vcf_ch.branch{ tool: it[0].id == "gridss" other: true} .set{input} From 3160d0dc27a4eaf8aeb91382c7ba977cf11d94d8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 11 Mar 2024 09:47:56 +0000 Subject: [PATCH 034/292] survivor stats for truth --- assets/svync/dragen.yaml | 65 +++++++++++++++++++ bin/add_svtype.py | 2 +- ...stics.nf => report_vcf_statistics_test.nf} | 4 +- .../local/report_vcf_statistics_truth.nf | 51 +++++++++++++++ workflows/variantbenchmarking.nf | 9 ++- 5 files changed, 125 insertions(+), 6 deletions(-) create mode 100644 assets/svync/dragen.yaml rename subworkflows/local/{report_vcf_statistics.nf => report_vcf_statistics_test.nf} (91%) create mode 100644 subworkflows/local/report_vcf_statistics_truth.nf diff --git a/assets/svync/dragen.yaml b/assets/svync/dragen.yaml new file mode 100644 index 0000000..8cd10ab --- /dev/null +++ b/assets/svync/dragen.yaml @@ -0,0 +1,65 @@ +id: dragen_$INFO/SVTYPE +info: + CALLER: + value: dragen + number: 1 + type: string + description: The caller used to determine this variant + SVLEN: + value: $INFO/SVLEN + number: 1 + type: integer + description: The length of the structural variant + alts: + INS: ~sum:~len:LEFT_SVINSSEQ,~len:RIGHT_SVINSSEQ + TRA: 1 + CIEND: + value: $INFO/CIEND + number: 2 + type: integer + description: PE confidence interval around END + CIPOS: + value: $INFO/CIPOS + number: 2 + type: integer + description: PE confidence interval around POS + SVTYPE: + value: $INFO/SVTYPE + number: 1 + type: string + description: Type of structural variant + CHR2: + value: + number: 1 + type: string + description: Chromosome for second position + alts: + TRA: $INFO/CHR2 + END: + value: $INFO/END + number: 1 + type: integer + description: End position of the structural variant + alts: + TRA: $INFO/POS2 + IMPRECISE: + value: $INFO/IMPRECISE + number: 0 + type: flag + description: Imprecise structural variation +format: + GT: + value: $FORMAT/GT + number: 1 + type: string + description: Genotype + PE: + value: $FORMAT/PR + number: 2 + type: integer + description: Paired-read support for the ref and alt alleles in the order listed + SR: + value: $FORMAT/SR + number: 2 + type: integer + description: Split-read support for the ref and alt alleles in the order listed diff --git a/bin/add_svtype.py b/bin/add_svtype.py index e2b5595..1ff6af9 100644 --- a/bin/add_svtype.py +++ b/bin/add_svtype.py @@ -12,7 +12,7 @@ parser = argparse.ArgumentParser() -parser.add_argument('--graph', required=True, help='Input VCF') +parser.add_argument('--input', required=True, help='Input VCF') parser.add_argument('--svlength', type=int, required=False, help='SV Lenght', default=50) args = parser.parse_args() diff --git a/subworkflows/local/report_vcf_statistics.nf b/subworkflows/local/report_vcf_statistics_test.nf similarity index 91% rename from subworkflows/local/report_vcf_statistics.nf rename to subworkflows/local/report_vcf_statistics_test.nf index 0dcf501..d4b1112 100644 --- a/subworkflows/local/report_vcf_statistics.nf +++ b/subworkflows/local/report_vcf_statistics_test.nf @@ -1,5 +1,5 @@ // -// PREPARE_VCFS: SUBWORKFLOW TO REPORT VCF STATS +// REPORT_VCF_STATISTICS_TEST: SUBWORKFLOW TO REPORT VCF STATS // params.options = [:] @@ -7,7 +7,7 @@ params.options = [:] include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' addParams( options: params.options ) include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' addParams( options: params.options ) -workflow REPORT_VCF_STATISTICS { +workflow REPORT_VCF_STATISTICS_TEST { take: input_ch // channel: [val(meta), vcf, index] diff --git a/subworkflows/local/report_vcf_statistics_truth.nf b/subworkflows/local/report_vcf_statistics_truth.nf new file mode 100644 index 0000000..cae5d30 --- /dev/null +++ b/subworkflows/local/report_vcf_statistics_truth.nf @@ -0,0 +1,51 @@ +// +// REPORT_VCF_STATISTICS_TRUTH: SUBWORKFLOW TO REPORT VCF STATS +// + +params.options = [:] + +include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' addParams( options: params.options ) +include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' addParams( options: params.options ) + +workflow REPORT_VCF_STATISTICS_TRUTH { + take: + input_ch // channel: [val(meta), vcf, index] + + main: + + versions=Channel.empty() + + // + // SURVIVOR_STATS + // + SURVIVOR_STATS( + input_ch.map{it -> tuple( it[0], it[1])}, + -1, + -1, + -1 + ) + survivor_stats = SURVIVOR_STATS.out.stats + versions = versions.mix(SURVIVOR_STATS.out.versions) + + // + // BCFTOOLS_STATS + // + BCFTOOLS_STATS( + input_ch, + [[],[]], + [[],[]], + [[],[]], + [[],[]], + [[],[]] + ) + bcftools_stats = BCFTOOLS_STATS.out.stats + versions = versions.mix(BCFTOOLS_STATS.out.versions) + + + // Add here a tool, to visualize SV statistics in a histogram. + + emit: + bcftools_stats + survivor_stats + versions +} diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index a8ec2ac..0f09505 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -28,8 +28,8 @@ include { SV_GERMLINE_BENCHMARK } from '../subworkflows/local/sv_germline_ben include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' include { SV_VCF_CONVERSIONS } from '../subworkflows/local/sv_vcf_conversion' -include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TEST } from '../subworkflows/local/report_vcf_statistics' -include { REPORT_VCF_STATISTICS as REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_vcf_statistics' +include { REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_vcf_statistics_truth.nf' +include { REPORT_STATISTICS_TEST } from '../subworkflows/local/report_vcf_statistics_test.nf' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -64,6 +64,7 @@ workflow VARIANTBENCHMARKING { : Channel.empty() // TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID + ch_samplesheet.branch{ sv: it[0].vartype == "sv" small: it[0].vartype == "small" @@ -113,10 +114,12 @@ workflow VARIANTBENCHMARKING { REPORT_STATISTICS_TEST( PREPARE_VCFS_TEST.out.vcf_ch ) + ch_versions = ch_versions.mix(REPORT_STATISTICS_TEST.out.versions) + REPORT_STATISTICS_TRUTH( PREPARE_VCFS_TRUTH.out.vcf_ch ) - ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) + ch_versions = ch_versions.mix(REPORT_STATISTICS_TRUTH.out.versions) // prepare benchmark set if (params.high_conf){ From 5c7e714fd7152876dc8f099120ca65eb86c4b76a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 11 Mar 2024 10:37:07 +0000 Subject: [PATCH 035/292] fix --- ...eport_vcf_statistics_test.nf => report_statistics_test.nf} | 4 ++-- ...ort_vcf_statistics_truth.nf => report_statistics_truth.nf} | 4 ++-- workflows/variantbenchmarking.nf | 4 ++-- 3 files changed, 6 insertions(+), 6 deletions(-) rename subworkflows/local/{report_vcf_statistics_test.nf => report_statistics_test.nf} (91%) rename subworkflows/local/{report_vcf_statistics_truth.nf => report_statistics_truth.nf} (90%) diff --git a/subworkflows/local/report_vcf_statistics_test.nf b/subworkflows/local/report_statistics_test.nf similarity index 91% rename from subworkflows/local/report_vcf_statistics_test.nf rename to subworkflows/local/report_statistics_test.nf index d4b1112..74bd35a 100644 --- a/subworkflows/local/report_vcf_statistics_test.nf +++ b/subworkflows/local/report_statistics_test.nf @@ -1,5 +1,5 @@ // -// REPORT_VCF_STATISTICS_TEST: SUBWORKFLOW TO REPORT VCF STATS +// REPORT_STATISTICS_TEST: SUBWORKFLOW TO REPORT VCF STATS // params.options = [:] @@ -7,7 +7,7 @@ params.options = [:] include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' addParams( options: params.options ) include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' addParams( options: params.options ) -workflow REPORT_VCF_STATISTICS_TEST { +workflow REPORT_STATISTICS_TEST { take: input_ch // channel: [val(meta), vcf, index] diff --git a/subworkflows/local/report_vcf_statistics_truth.nf b/subworkflows/local/report_statistics_truth.nf similarity index 90% rename from subworkflows/local/report_vcf_statistics_truth.nf rename to subworkflows/local/report_statistics_truth.nf index cae5d30..2cdeab1 100644 --- a/subworkflows/local/report_vcf_statistics_truth.nf +++ b/subworkflows/local/report_statistics_truth.nf @@ -1,5 +1,5 @@ // -// REPORT_VCF_STATISTICS_TRUTH: SUBWORKFLOW TO REPORT VCF STATS +// REPORT_STATISTICS_TRUTH: SUBWORKFLOW TO REPORT VCF STATS // params.options = [:] @@ -7,7 +7,7 @@ params.options = [:] include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' addParams( options: params.options ) include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' addParams( options: params.options ) -workflow REPORT_VCF_STATISTICS_TRUTH { +workflow REPORT_STATISTICS_TRUTH { take: input_ch // channel: [val(meta), vcf, index] diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 0f09505..c3c9be0 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -28,8 +28,8 @@ include { SV_GERMLINE_BENCHMARK } from '../subworkflows/local/sv_germline_ben include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' include { SV_VCF_CONVERSIONS } from '../subworkflows/local/sv_vcf_conversion' -include { REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_vcf_statistics_truth.nf' -include { REPORT_STATISTICS_TEST } from '../subworkflows/local/report_vcf_statistics_test.nf' +include { REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_statistics_truth.nf' +include { REPORT_STATISTICS_TEST } from '../subworkflows/local/report_statistics_test.nf' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From 551c28363a0f098df5d538cbfba7e1b2151ee080 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 11 Mar 2024 11:05:34 +0000 Subject: [PATCH 036/292] truvari lint fix --- modules/nf-core/survivor/filter/main.nf | 6 +- .../svanalyzer/svbenchmark/environment.yml | 3 +- .../nf-core/svanalyzer/svbenchmark/main.nf | 50 ++- .../nf-core/svanalyzer/svbenchmark/meta.yml | 11 +- .../svanalyzer/svbenchmark/tests/main.nf.test | 20 +- .../svbenchmark/tests/main.nf.test.snap | 290 +++++++----------- modules/nf-core/truvari/bench/main.nf | 31 +- subworkflows/local/sv_germline_benchmark.nf | 3 +- 8 files changed, 201 insertions(+), 213 deletions(-) diff --git a/modules/nf-core/survivor/filter/main.nf b/modules/nf-core/survivor/filter/main.nf index d14ba0a..c806104 100644 --- a/modules/nf-core/survivor/filter/main.nf +++ b/modules/nf-core/survivor/filter/main.nf @@ -8,15 +8,15 @@ process SURVIVOR_FILTER { 'biocontainers/survivor:1.0.7--h9a82719_1' }" input: - tuple val(meta),path(vcf_file), path(bed) // VCF file to filter and BED file with regions to ignore (NA to disable) + tuple val(meta), path(vcf_file), path(bed) // VCF file to filter and BED file with regions to ignore (NA to disable) val(minsv) // Min SV size (-1 to disable) val(maxsv) // Max SV size (-1 to disable) val(minallelefreq) // Min allele frequency (0-1) val(minnumreads) // Min number of reads support: RE flag (-1 to disable) output: - tuple val(meta),path("*.vcf"), emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.vcf"), emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/svanalyzer/svbenchmark/environment.yml b/modules/nf-core/svanalyzer/svbenchmark/environment.yml index 12cac54..2e20a2c 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/environment.yml +++ b/modules/nf-core/svanalyzer/svbenchmark/environment.yml @@ -6,5 +6,4 @@ channels: - defaults dependencies: - - bioconda::edlib=1.2.3 - - bioconda::svanalyzer=0.35 + - bioconda::svanalyzer=0.36 diff --git a/modules/nf-core/svanalyzer/svbenchmark/main.nf b/modules/nf-core/svanalyzer/svbenchmark/main.nf index 5d2d079..37ecf4d 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/main.nf +++ b/modules/nf-core/svanalyzer/svbenchmark/main.nf @@ -1,33 +1,42 @@ process SVANALYZER_SVBENCHMARK { tag "$meta.id" - label 'process_medium' + label 'process_single' - conda "bioconda::svanalyzer=0.35" + //Conda is not supported at the moment: https://github.com/bioconda/bioconda-recipes/issues/37646 + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/svanalyzer:0.35--pl526_0': - 'biocontainers/svanalyzer:0.35--pl526_0' }" + 'https://depot.galaxyproject.org/singularity/svanalyzer:0.36--pl526_0': + 'biocontainers/svanalyzer:0.36--pl526_0' }" input: - tuple val(meta),path(test), path(test_index), path(truth), path(truth_index), path(bed) - tuple path(fasta), path(fai) + tuple val(meta), path(test) + tuple val(meta2),path(truth) + tuple val(meta3),path(fasta) + tuple val(meta4),path(fai) + tuple val(meta5),path(bed) output: - tuple val(meta),path("*.falsenegatives.vcf"), emit: fns - tuple val(meta),path("*.falsepositives.vcf"), emit: fps - tuple val(meta),path("*.distances") , emit: distances - tuple val(meta),path("*.log") , emit: log - tuple val(meta), path("*.report") , emit: report - path "versions.yml" , emit: versions + tuple val(meta), path("*.falsenegatives.vcf.gz"), emit: fns + tuple val(meta), path("*.falsepositives.vcf.gz"), emit: fps + tuple val(meta), path("*.distances") , emit: distances + tuple val(meta), path("*.log") , emit: log + tuple val(meta), path("*.report") , emit: report + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "SVANALYZER_SVBENCHMARK module does not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def bed = bed ? "-includebed $bed" : "" - def VERSION = '0.35' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + def VERSION = '0.36' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ svanalyzer \\ @@ -39,6 +48,9 @@ process SVANALYZER_SVBENCHMARK { --prefix $prefix \\ $bed + bgzip ${args2} --threads ${task.cpus} -c ${prefix}.falsenegatives.vcf > ${prefix}.falsenegatives.vcf.gz + bgzip ${args2} --threads ${task.cpus} -c ${prefix}.falsepositives.vcf > ${prefix}.falsepositives.vcf.gz + cat <<-END_VERSIONS > versions.yml "${task.process}": svanalyzer: ${VERSION} @@ -46,13 +58,17 @@ process SVANALYZER_SVBENCHMARK { """ stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "SVANALYZER_SVBENCHMARK module does not support Conda. Please use Docker / Singularity / Podman instead." + } def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def VERSION = '0.35' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + def VERSION = '0.36' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ - touch ${prefix}.falsenegatives.vcf - touch ${prefix}.falsepositives.vcf + touch ${prefix}.falsenegatives.vcf.gz + touch ${prefix}.falsepositives.vcf.gz touch ${prefix}.distances touch ${prefix}.log touch ${prefix}.report diff --git a/modules/nf-core/svanalyzer/svbenchmark/meta.yml b/modules/nf-core/svanalyzer/svbenchmark/meta.yml index ba72c3d..08b2048 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/meta.yml +++ b/modules/nf-core/svanalyzer/svbenchmark/meta.yml @@ -30,6 +30,11 @@ input: description: | Groovy Map containing reference genome information e.g. `[ id:'test3' ]` + - meta4: + type: map + description: | + Groovy Map containing bed file information + e.g. `[ id:'test4' ]` - test: type: file description: A VCF-formatted file of structural variants to test (required) @@ -59,11 +64,11 @@ output: - fns: type: file description: VCF file with False Negatives - pattern: "*.{vcf}" + pattern: "*.{vcf.gz}" - fps: type: file description: VCF file with False Positives - pattern: "*.{vcf}" + pattern: "*.{vcf.gz}" - distances: type: file description: TSV file with genomic distances and size differences between structural variants compared @@ -75,6 +80,6 @@ output: - report: type: file description: Text file reporting RECALL, PRECISION and F1. - pattern: "*.{log}" + pattern: "*.{report}" authors: - "@kubranarci" diff --git a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test index 51466f7..6262df2 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test +++ b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process SVANALYZER_SVBENCHMARK" script "../main.nf" process "SVANALYZER_SVBENCHMARK" + tag "modules" tag "modules_nfcore" tag "svanalyzer" @@ -11,6 +12,9 @@ nextflow_process { test("homo_sapiens - illumina - vcf.gz") { when { + params { + outdir = $outputDir + } process { """ input[0] = [ @@ -22,12 +26,14 @@ nextflow_process { file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true) ] input[2] = [ + [ id:'test3' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) ] input[3] = [ + [ id:'test4' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) ] - input[4] = [] + input[4] = [[],[]] """ } } @@ -41,13 +47,16 @@ nextflow_process { { assert snapshot(process.out.report).match("report") }, { assert snapshot(process.out.versions).match("versions") } ) + } } - test("homo_sapiens - illumina - vcf.gz - bed") { when { + params { + outdir = $outputDir + } process { """ input[0] = [ @@ -59,12 +68,15 @@ nextflow_process { file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true) ] input[2] = [ + [ id:'test3' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) ] input[3] = [ + [ id:'test4' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) ] input[4] = [ + [ id:'test5' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true) ] """ @@ -81,5 +93,7 @@ nextflow_process { { assert snapshot(process.out.versions).match("bed_versions") } ) } + } -} \ No newline at end of file + +} diff --git a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap index 599697d..a325104 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap +++ b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap @@ -1,188 +1,122 @@ { - "homo_sapiens - illumina - vcf.gz": { + "distances": { "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.falsenegatives.vcf:md5,7a574ad3c614f0c38a6dccb5901ddc05" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" - ] - ], - "2": [ - [ - { - "id": "test" - }, - "test.distances:md5,b6a908b1187f159429dc8c6beb6e6f57" - ] - ], - "3": [ - [ - { - "id": "test" - }, - "test.log:md5,16fd250ae2adcb07c8f101f640fdce81" - ] - ], - "4": [ - [ - { - "id": "test" - }, - "test.report:md5,d0c7db2466442cbdd4f87ad516e00d32" - ] - ], - "5": [ - "versions.yml:md5,6ac08491dbe591d24615c131658cf771" - ], - "distances": [ - [ - { - "id": "test" - }, - "test.distances:md5,b6a908b1187f159429dc8c6beb6e6f57" - ] - ], - "fns": [ - [ - { - "id": "test" - }, - "test.falsenegatives.vcf:md5,7a574ad3c614f0c38a6dccb5901ddc05" - ] - ], - "fps": [ - [ - { - "id": "test" - }, - "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" - ] - ], - "log": [ - [ - { - "id": "test" - }, - "test.log:md5,16fd250ae2adcb07c8f101f640fdce81" - ] - ], - "report": [ - [ - { - "id": "test" - }, - "test.report:md5,d0c7db2466442cbdd4f87ad516e00d32" - ] - ], - "versions": [ - "versions.yml:md5,6ac08491dbe591d24615c131658cf771" + [ + [ + { + "id": "test" + }, + "test.distances:md5,b6a908b1187f159429dc8c6beb6e6f57" ] - } + ] ], - "timestamp": "2023-11-24T15:28:29.422156227" + "timestamp": "2023-11-28T09:41:26.099766436" }, - "homo_sapiens - illumina - vcf.gz - bed": { + "bed_fps": { "content": [ - { - "0": [ - [ - { - "id": "test" - }, - "test.falsenegatives.vcf:md5,c04105833d5f9acb5ec50af0b89a6b0f" - ] - ], - "1": [ - [ - { - "id": "test" - }, - "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" - ] - ], - "2": [ - [ - { - "id": "test" - }, - "test.distances:md5,95f7b1679e805979f0c9a4a322cb77f4" - ] - ], - "3": [ - [ - { - "id": "test" - }, - "test.log:md5,75f05aac48afd818f2d5e8a3144116ea" - ] - ], - "4": [ - [ - { - "id": "test" - }, - "test.report:md5,aaa9225dd6e261951c020e23473375b9" - ] - ], - "5": [ - "versions.yml:md5,6ac08491dbe591d24615c131658cf771" - ], - "distances": [ - [ - { - "id": "test" - }, - "test.distances:md5,95f7b1679e805979f0c9a4a322cb77f4" - ] - ], - "fns": [ - [ - { - "id": "test" - }, - "test.falsenegatives.vcf:md5,c04105833d5f9acb5ec50af0b89a6b0f" - ] - ], - "fps": [ - [ - { - "id": "test" - }, - "test.falsepositives.vcf:md5,146f05125330a9c0c4581a5a581744a7" - ] - ], - "log": [ - [ - { - "id": "test" - }, - "test.log:md5,75f05aac48afd818f2d5e8a3144116ea" - ] - ], - "report": [ - [ - { - "id": "test" - }, - "test.report:md5,aaa9225dd6e261951c020e23473375b9" - ] - ], - "versions": [ - "versions.yml:md5,6ac08491dbe591d24615c131658cf771" + [ + [ + { + "id": "test" + }, + "test.falsepositives.vcf.gz:md5,b62485bcf0464d385c56bb438a79e48b" ] - } + ] ], - "timestamp": "2023-11-24T15:29:05.547215282" + "timestamp": "2023-11-28T09:42:17.173871972" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,b9248741a9510f3e9d231d94ee27eb77" + ] + ], + "timestamp": "2023-11-28T09:41:26.131817496" + }, + "bed_distances": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.distances:md5,95f7b1679e805979f0c9a4a322cb77f4" + ] + ] + ], + "timestamp": "2023-11-28T09:42:17.186737442" + }, + "bed_versions": { + "content": [ + [ + "versions.yml:md5,b9248741a9510f3e9d231d94ee27eb77" + ] + ], + "timestamp": "2023-11-28T09:42:17.210528341" + }, + "bed_fns": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.falsenegatives.vcf.gz:md5,c2963b25bdca28996ca1c0eb61498fca" + ] + ] + ], + "timestamp": "2023-11-28T09:42:17.159257423" + }, + "fps": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.falsepositives.vcf.gz:md5,b62485bcf0464d385c56bb438a79e48b" + ] + ] + ], + "timestamp": "2023-11-28T09:41:26.093398936" + }, + "report": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.report:md5,d0c7db2466442cbdd4f87ad516e00d32" + ] + ] + ], + "timestamp": "2023-11-28T09:41:26.123752116" + }, + "fns": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.falsenegatives.vcf.gz:md5,5d637034f638b3338390c4047b461e91" + ] + ] + ], + "timestamp": "2023-11-28T09:41:26.075866677" + }, + "bed_report": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.report:md5,aaa9225dd6e261951c020e23473375b9" + ] + ] + ], + "timestamp": "2023-11-28T09:42:17.198219302" } } \ No newline at end of file diff --git a/modules/nf-core/truvari/bench/main.nf b/modules/nf-core/truvari/bench/main.nf index 4e1a104..8b0c509 100644 --- a/modules/nf-core/truvari/bench/main.nf +++ b/modules/nf-core/truvari/bench/main.nf @@ -5,11 +5,12 @@ process TRUVARI_BENCH { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/truvari:4.1.0--pyhdfd78af_0': - 'quay.io/biocontainers/truvari:4.1.0--pyhdfd78af_0' }" + 'biocontainers/truvari:4.1.0--pyhdfd78af_0' }" input: - tuple val(meta),path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) - tuple path(fasta), path(fai) + tuple val(meta), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) output: tuple val(meta), path("*.fn.vcf.gz") , emit: fn_vcf @@ -30,7 +31,6 @@ process TRUVARI_BENCH { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def regions = bed ? "--includebed $bed" : "" - def convert_type = params.dup_to_ins ? "--dup-to-ins" : "" """ truvari bench \\ @@ -38,8 +38,6 @@ process TRUVARI_BENCH { --comp ${vcf} \\ --reference ${fasta} \\ --output ${prefix} \\ - --pctseq $params.similarity \\ - $convert_type \\ ${regions} \\ ${args} @@ -58,4 +56,25 @@ process TRUVARI_BENCH { truvari: \$(echo \$(truvari version 2>&1) | sed 's/^Truvari v//' )) END_VERSIONS """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.fn.vcf.gz + touch ${prefix}.fn.vcf.gz.tbi + touch ${prefix}.fp.vcf.gz + touch ${prefix}.fp.vcf.gz.tbi + touch ${prefix}.tp-base.vcf.gz + touch ${prefix}.tp-base.vcf.gz.tbi + touch ${prefix}.tp-comp.vcf.gz + touch ${prefix}.tp-comp.vcf.gz.tbi + touch ${prefix}.summary.json + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + truvari: \$(echo \$(truvari version 2>&1) | sed 's/^Truvari v//' )) + END_VERSIONS + """ } diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index 2e4cc0c..b0b173b 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -38,7 +38,8 @@ workflow SV_GERMLINE_BENCHMARK { // TRUVARI_BENCH( input_ch, - ref + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) }, + ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } ) versions = versions.mix(TRUVARI_BENCH.out.versions) From 1e3679d36f72778c674ebe6b505bc85b8c354fb1 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 11 Mar 2024 13:11:59 +0000 Subject: [PATCH 037/292] fixes module lint --- modules.json | 2 +- .../nf-core/svanalyzer/svbenchmark/main.nf | 8 +- .../nf-core/svanalyzer/svbenchmark/meta.yml | 17 +++-- .../svanalyzer/svbenchmark/tests/main.nf.test | 33 ++++---- .../svbenchmark/tests/main.nf.test.snap | 76 ++++++++++++++----- subworkflows/local/sv_germline_benchmark.nf | 3 +- 6 files changed, 88 insertions(+), 51 deletions(-) diff --git a/modules.json b/modules.json index 06400f3..07aaaee 100644 --- a/modules.json +++ b/modules.json @@ -42,7 +42,7 @@ }, "svanalyzer/svbenchmark": { "branch": "master", - "git_sha": "419065b3a66b857af8b49d41dc1ba3858821da22", + "git_sha": "8a2dd9e84cde68ebba45fb2c602c312c999c02a0", "installed_by": ["modules"] }, "svync": { diff --git a/modules/nf-core/svanalyzer/svbenchmark/main.nf b/modules/nf-core/svanalyzer/svbenchmark/main.nf index 37ecf4d..e76829e 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/main.nf +++ b/modules/nf-core/svanalyzer/svbenchmark/main.nf @@ -10,11 +10,9 @@ process SVANALYZER_SVBENCHMARK { 'biocontainers/svanalyzer:0.36--pl526_0' }" input: - tuple val(meta), path(test) - tuple val(meta2),path(truth) - tuple val(meta3),path(fasta) - tuple val(meta4),path(fai) - tuple val(meta5),path(bed) + tuple val(meta), path(test), path(test_tbi), path(truth), path(truth_tbi), path(bed) + tuple val(meta2),path(fasta) + tuple val(meta3),path(fai) output: tuple val(meta), path("*.falsenegatives.vcf.gz"), emit: fns diff --git a/modules/nf-core/svanalyzer/svbenchmark/meta.yml b/modules/nf-core/svanalyzer/svbenchmark/meta.yml index 08b2048..c8a76ac 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/meta.yml +++ b/modules/nf-core/svanalyzer/svbenchmark/meta.yml @@ -23,26 +23,29 @@ input: - meta2: type: map description: | - Groovy Map containing truth sample information + Groovy Map containing reference genome information for fasta e.g. `[ id:'test2' ]` - meta3: type: map description: | - Groovy Map containing reference genome information + Groovy Map containing reference genome information for fai e.g. `[ id:'test3' ]` - - meta4: - type: map - description: | - Groovy Map containing bed file information - e.g. `[ id:'test4' ]` - test: type: file description: A VCF-formatted file of structural variants to test (required) pattern: "*.{vcf,vcf.gz}" + - test_tbi: + type: file + description: A VCF-formatted file index of structural variants to test only for zipped files + pattern: "*.{vcf.gz.tbi}" - truth: type: file description: A VCF-formatted file of variants to compare against (required) pattern: "*.{vcf,vcf.gz}" + - truth_tbi: + type: file + description: A VCF-formatted file of variants to compare against only for zipped files + pattern: "*.{vcf.gz.tbi}" - fasta: type: file description: The reference FASTA file for the supplied VCF file or files (required) diff --git a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test index 6262df2..469fe8c 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test +++ b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test @@ -9,7 +9,7 @@ nextflow_process { tag "svanalyzer" tag "svanalyzer/svbenchmark" - test("homo_sapiens - illumina - vcf.gz") { + test("homo_sapiens - illumina - vcf.gz - bed") { when { params { @@ -19,21 +19,20 @@ nextflow_process { """ input[0] = [ [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true) + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true) ] input[1] = [ [ id:'test2' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true) - ] - input[2] = [ - [ id:'test3' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) ] - input[3] = [ + input[2] = [ [ id:'test4' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) ] - input[4] = [[],[]] """ } } @@ -51,7 +50,7 @@ nextflow_process { } } - test("homo_sapiens - illumina - vcf.gz - bed") { + test("homo_sapiens - illumina - vcf.gz") { when { params { @@ -61,24 +60,20 @@ nextflow_process { """ input[0] = [ [ id:'test' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true) + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz_tbi'], checkIfExists: true), + [] ] input[1] = [ [ id:'test2' ], // meta map - file(params.test_data['homo_sapiens']['illumina']['test2_haplotc_vcf_gz'], checkIfExists: true) + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) ] input[2] = [ [ id:'test3' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - input[3] = [ - [ id:'test4' ], // meta map file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) ] - input[4] = [ - [ id:'test5' ], // meta map - file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true) - ] """ } } diff --git a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap index a325104..f31af23 100644 --- a/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap +++ b/modules/nf-core/svanalyzer/svbenchmark/tests/main.nf.test.snap @@ -6,11 +6,15 @@ { "id": "test" }, - "test.distances:md5,b6a908b1187f159429dc8c6beb6e6f57" + "test.distances:md5,95f7b1679e805979f0c9a4a322cb77f4" ] ] ], - "timestamp": "2023-11-28T09:41:26.099766436" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:11.509970731" }, "bed_fps": { "content": [ @@ -19,11 +23,15 @@ { "id": "test" }, - "test.falsepositives.vcf.gz:md5,b62485bcf0464d385c56bb438a79e48b" + "test.falsepositives.vcf.gz:md5,146f05125330a9c0c4581a5a581744a7" ] ] ], - "timestamp": "2023-11-28T09:42:17.173871972" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:50.064755761" }, "versions": { "content": [ @@ -31,7 +39,11 @@ "versions.yml:md5,b9248741a9510f3e9d231d94ee27eb77" ] ], - "timestamp": "2023-11-28T09:41:26.131817496" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:11.662700758" }, "bed_distances": { "content": [ @@ -40,11 +52,15 @@ { "id": "test" }, - "test.distances:md5,95f7b1679e805979f0c9a4a322cb77f4" + "test.distances:md5,b6a908b1187f159429dc8c6beb6e6f57" ] ] ], - "timestamp": "2023-11-28T09:42:17.186737442" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:50.134445649" }, "bed_versions": { "content": [ @@ -52,7 +68,11 @@ "versions.yml:md5,b9248741a9510f3e9d231d94ee27eb77" ] ], - "timestamp": "2023-11-28T09:42:17.210528341" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:50.348992284" }, "bed_fns": { "content": [ @@ -61,11 +81,15 @@ { "id": "test" }, - "test.falsenegatives.vcf.gz:md5,c2963b25bdca28996ca1c0eb61498fca" + "test.falsenegatives.vcf.gz:md5,7a574ad3c614f0c38a6dccb5901ddc05" ] ] ], - "timestamp": "2023-11-28T09:42:17.159257423" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:49.998007508" }, "fps": { "content": [ @@ -74,11 +98,15 @@ { "id": "test" }, - "test.falsepositives.vcf.gz:md5,b62485bcf0464d385c56bb438a79e48b" + "test.falsepositives.vcf.gz:md5,146f05125330a9c0c4581a5a581744a7" ] ] ], - "timestamp": "2023-11-28T09:41:26.093398936" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:11.432409299" }, "report": { "content": [ @@ -87,11 +115,15 @@ { "id": "test" }, - "test.report:md5,d0c7db2466442cbdd4f87ad516e00d32" + "test.report:md5,aaa9225dd6e261951c020e23473375b9" ] ] ], - "timestamp": "2023-11-28T09:41:26.123752116" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:11.587407703" }, "fns": { "content": [ @@ -100,11 +132,15 @@ { "id": "test" }, - "test.falsenegatives.vcf.gz:md5,5d637034f638b3338390c4047b461e91" + "test.falsenegatives.vcf.gz:md5,c04105833d5f9acb5ec50af0b89a6b0f" ] ] ], - "timestamp": "2023-11-28T09:41:26.075866677" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:11.334428964" }, "bed_report": { "content": [ @@ -113,10 +149,14 @@ { "id": "test" }, - "test.report:md5,aaa9225dd6e261951c020e23473375b9" + "test.report:md5,d0c7db2466442cbdd4f87ad516e00d32" ] ] ], - "timestamp": "2023-11-28T09:42:17.198219302" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-11T12:16:50.202872842" } } \ No newline at end of file diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index b0b173b..75be720 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -52,7 +52,8 @@ workflow SV_GERMLINE_BENCHMARK { // slower than truvari SVANALYZER_SVBENCHMARK( input_ch, - ref + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) }, + ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } ) versions = versions.mix(SVANALYZER_SVBENCHMARK.out.versions) From 3f980c5680f6ee3d663de9aa14c1c3580d37f293 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 11 Mar 2024 16:37:20 +0000 Subject: [PATCH 038/292] rtgtools and happy added --- assets/samplesheet_small.csv | 2 + conf/modules.config | 20 ++- conf/test_small.config | 36 ++++++ modules.json | 15 +++ modules/nf-core/happy/happy/environment.yml | 7 ++ modules/nf-core/happy/happy/main.nf | 84 +++++++++++++ modules/nf-core/happy/happy/meta.yml | 114 ++++++++++++++++++ .../nf-core/rtgtools/format/environment.yml | 7 ++ modules/nf-core/rtgtools/format/main.nf | 66 ++++++++++ modules/nf-core/rtgtools/format/meta.yml | 51 ++++++++ .../nf-core/rtgtools/vcfeval/environment.yml | 7 ++ modules/nf-core/rtgtools/vcfeval/main.nf | 90 ++++++++++++++ modules/nf-core/rtgtools/vcfeval/meta.yml | 112 +++++++++++++++++ subworkflows/local/prepare_vcfs_test.nf | 4 +- .../local/small_germline_benchmark.nf | 54 +++++++++ workflows/variantbenchmarking.nf | 11 ++ 16 files changed, 676 insertions(+), 4 deletions(-) create mode 100644 assets/samplesheet_small.csv create mode 100644 conf/test_small.config create mode 100644 modules/nf-core/happy/happy/environment.yml create mode 100644 modules/nf-core/happy/happy/main.nf create mode 100644 modules/nf-core/happy/happy/meta.yml create mode 100644 modules/nf-core/rtgtools/format/environment.yml create mode 100644 modules/nf-core/rtgtools/format/main.nf create mode 100644 modules/nf-core/rtgtools/format/meta.yml create mode 100644 modules/nf-core/rtgtools/vcfeval/environment.yml create mode 100644 modules/nf-core/rtgtools/vcfeval/main.nf create mode 100644 modules/nf-core/rtgtools/vcfeval/meta.yml create mode 100644 subworkflows/local/small_germline_benchmark.nf diff --git a/assets/samplesheet_small.csv b/assets/samplesheet_small.csv new file mode 100644 index 0000000..965d260 --- /dev/null +++ b/assets/samplesheet_small.csv @@ -0,0 +1,2 @@ +test_vcf,caller,vartype +"https://raw.githubusercontent.com/nf-core/test-datasets/modules//data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",haplotypecaller,small diff --git a/conf/modules.config b/conf/modules.config index 50e4f87..df98a46 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -113,6 +113,24 @@ process { mode: params.publish_dir_mode ] } + withName: "RTGTOOLS_VCFEVAL" { + ext.prefix = {"${params.sample}"} + //ext.args = {"--ref-overlap --all-record --output-mode ga4gh"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/rtgtools_bench"}, + pattern: "*{.vcf.gz,vcf.gz.tbi,tsv.gz,txt}", + mode: params.publish_dir_mode + ] + } + withName: "HAPPY_HAPPY" { + ext.prefix = {"${params.sample}"} + //ext.args = {""} + publishDir = [ + path: {"${params.outdir}/${meta.id}/happy_bench"}, + pattern: "*{.csv.gz,csv,json.gz,json,vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } withName: "TRUVARI_BENCH" { ext.prefix = {"${params.sample}"} ext.args = {"--pctsize 0.5 --pctovl 0.5 --refdist 1000 --pick ac"} @@ -186,7 +204,7 @@ process { // Don't publish results for these processes // process { - withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX|TABIX_BGZIP' { + withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX|TABIX_BGZIP|RTGTOOLS_FORMAT' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false diff --git a/conf/test_small.config b/conf/test_small.config new file mode 100644 index 0000000..8c505eb --- /dev/null +++ b/conf/test_small.config @@ -0,0 +1,36 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 16 + max_memory = '100.GB' + max_time = '8.h' + + // Input data + sample = "test" + input = "assets/samplesheet_small.csv" + + // Genome references + genome = 'hg38' + + // Processes + analysis = 'germline' + method = 'rtgtools,happy' // + preprocess = "normalization, deduplication" + //min_sv_size = 50 + + truth = "https://raw.githubusercontent.com/nf-core/test-datasets/modules//data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz" +} diff --git a/modules.json b/modules.json index 07aaaee..5822726 100644 --- a/modules.json +++ b/modules.json @@ -20,6 +20,11 @@ "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, + "happy/happy": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, "manta/convertinversion": { "branch": "master", "git_sha": "1e2b7fb7106852388610c0360d234b0829eb980e", @@ -30,6 +35,16 @@ "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] }, + "rtgtools/format": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, + "rtgtools/vcfeval": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, "survivor/filter": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", diff --git a/modules/nf-core/happy/happy/environment.yml b/modules/nf-core/happy/happy/environment.yml new file mode 100644 index 0000000..3d29dcb --- /dev/null +++ b/modules/nf-core/happy/happy/environment.yml @@ -0,0 +1,7 @@ +name: happy_happy +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::hap.py=0.3.14 diff --git a/modules/nf-core/happy/happy/main.nf b/modules/nf-core/happy/happy/main.nf new file mode 100644 index 0000000..b3cc12d --- /dev/null +++ b/modules/nf-core/happy/happy/main.nf @@ -0,0 +1,84 @@ +process HAPPY_HAPPY { + tag "$meta.id" + label 'process_medium' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/hap.py:0.3.14--py27h5c5a3ab_0': + 'biocontainers/hap.py:0.3.14--py27h5c5a3ab_0' }" + + input: + tuple val(meta), path(query_vcf), path(truth_vcf), path(regions_bed), path(targets_bed) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fasta_fai) + tuple val(meta4), path(false_positives_bed) + tuple val(meta5), path(stratification_tsv) + tuple val(meta6), path(stratification_beds) + + output: + tuple val(meta), path('*.summary.csv') , emit: summary_csv + tuple val(meta), path('*.roc.all.csv.gz') , emit: roc_all_csv + tuple val(meta), path('*.roc.Locations.INDEL.csv.gz') , emit: roc_indel_locations_csv + tuple val(meta), path('*.roc.Locations.INDEL.PASS.csv.gz') , emit: roc_indel_locations_pass_csv + tuple val(meta), path('*.roc.Locations.SNP.csv.gz') , emit: roc_snp_locations_csv + tuple val(meta), path('*.roc.Locations.SNP.PASS.csv.gz') , emit: roc_snp_locations_pass_csv + tuple val(meta), path('*.extended.csv') , emit: extended_csv + tuple val(meta), path('*.runinfo.json') , emit: runinfo + tuple val(meta), path('*.metrics.json.gz') , emit: metrics_json + tuple val(meta), path('*.vcf.gz') , emit: vcf, optional:true + tuple val(meta), path('*.tbi') , emit: tbi, optional:true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions = regions_bed ? "-R ${regions_bed}" : "" + def targets = targets_bed ? "-T ${targets_bed}" : "" + def false_positives = false_positives_bed ? "--false-positives ${false_positives_bed}" : "" + def stratification = stratification_tsv ? "--stratification ${stratification_tsv}" : "" + def VERSION = '0.3.14' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + hap.py \\ + ${truth_vcf} \\ + ${query_vcf} \\ + ${args} \\ + --reference ${fasta} \\ + --threads ${task.cpus} \\ + ${regions} \\ + ${targets} \\ + ${false_positives} \\ + ${stratification} \\ + -o ${prefix} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + hap.py: $VERSION + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def VERSION = '0.3.14' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + touch ${prefix}.summary.csv + touch ${prefix}.roc.all.csv.gz + touch ${prefix}.roc.Locations.INDEL.csv.gz + touch ${prefix}.roc.Locations.INDEL.PASS.csv.gz + touch ${prefix}.roc.Locations.SNP.csv.gz + touch ${prefix}.roc.Locations.SNP.PASS.csv.gz + touch ${prefix}.extended.csv + touch ${prefix}.runinfo.json + touch ${prefix}.metrics.json.gz + touch ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + hap.py: $VERSION + END_VERSIONS + """ +} diff --git a/modules/nf-core/happy/happy/meta.yml b/modules/nf-core/happy/happy/meta.yml new file mode 100644 index 0000000..85912cb --- /dev/null +++ b/modules/nf-core/happy/happy/meta.yml @@ -0,0 +1,114 @@ +name: "happy_happy" +description: Hap.py is a tool to compare diploid genotypes at haplotype level. Rather than comparing VCF records row by row, hap.py will generate and match alternate sequences in a superlocus. A superlocus is a small region of the genome (sized between 1 and around 1000 bp) that contains one or more variants. +keywords: + - happy + - benchmark + - haplotype + - validation +tools: + - "happy": + description: "Haplotype VCF comparison tools" + homepage: "https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub/apps/hap-py-benchmarking.html" + documentation: "https://github.com/Illumina/hap.py" + tool_dev_url: "https://github.com/Illumina/hap.py" + licence: "['BSD-2-clause']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - query_vcf: + type: file + description: VCF/GVCF file to query + pattern: "*.{gvcf,vcf}.gz" + - truth_vcf: + type: file + description: gold standard VCF file + pattern: "*.{gvcf,vcf}.gz" + - regions_bed: + type: file + description: Sparse regions to restrict the analysis to + pattern: "*.bed" + - targets_bed: + type: file + description: Dense regions to restrict the analysis to + pattern: "*.bed" + - fasta: + type: file + description: FASTA file of the reference genome + pattern: "*.{fa,fasta}" + - fasta_fai: + type: file + description: The index of the reference FASTA + pattern: "*.fai" + - false_positives_bed: + type: file + description: False positive / confident call regions. Calls outside these regions will be labelled as UNK. + pattern: "*.{bed,bed.gz}" + - stratification_tsv: + type: file + description: Stratification file list in TSV format + pattern: "*.tsv" + - stratification_beds: + type: file(s) + description: One or more BED files used for stratification (these should be referenced in the stratification TSV) + pattern: "*.bed" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - summary_csv: + type: file + description: A CSV file containing the summary of the benchmarking + pattern: "*.summary.csv" + - roc_all_csv: + type: file + description: A CSV file containing ROC values for all variants + pattern: "*.roc.all.csv.gz" + - roc_indel_locations_csv: + type: file + description: A CSV file containing ROC values for all indels + pattern: "*.roc.Locations.INDEL.csv.gz" + - roc_indel_locations_pass_csv: + type: file + description: A CSV file containing ROC values for all indels that passed all filters + pattern: "*.roc.Locations.INDEL.PASS.csv.gz" + - roc_snp_locations_csv: + type: file + description: A CSV file containing ROC values for all SNPs + pattern: "*.roc.Locations.SNP.csv.gz" + - roc_snp_locations_pass_csv: + type: file + description: A CSV file containing ROC values for all SNPs that passed all filters + pattern: "*.roc.Locations.SNP.PASS.csv.gz" + - extended_csv: + type: file + description: A CSV file containing extended info of the benchmarking + pattern: "*.extended.csv" + - json: + type: file + description: A JSON file containing the run info + pattern: "*.runinfo.json" + - runinfo: + type: file + description: A JSON file containing the benchmarking metrics + pattern: "*.metrics.json.gz" + - vcf: + type: file + description: An annotated VCF + pattern: "*.vcf.gz" + - tbi: + type: file + description: The index of the annotated VCF + pattern: "*.tbi" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/modules/nf-core/rtgtools/format/environment.yml b/modules/nf-core/rtgtools/format/environment.yml new file mode 100644 index 0000000..15b3df5 --- /dev/null +++ b/modules/nf-core/rtgtools/format/environment.yml @@ -0,0 +1,7 @@ +name: rtgtools_format +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::rtg-tools=3.12.1 diff --git a/modules/nf-core/rtgtools/format/main.nf b/modules/nf-core/rtgtools/format/main.nf new file mode 100644 index 0000000..802d3b2 --- /dev/null +++ b/modules/nf-core/rtgtools/format/main.nf @@ -0,0 +1,66 @@ +process RTGTOOLS_FORMAT { + tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/rtg-tools:3.12.1--hdfd78af_0': + 'biocontainers/rtg-tools:3.12.1--hdfd78af_0' }" + + input: + tuple val(meta), path(input1), path(input2), path(sam_rg) + + output: + tuple val(meta), path("*.sdf"), emit: sdf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def single = meta.containsKey("single_end") ? meta.single_end : true + + def input = single ? "${input1}" : "--left ${input1} --right ${input2}" + def rg = sam_rg ? "--sam-rg ${sam_rg}" : "" + + def avail_mem = "3G" + if (!task.memory) { + log.info '[RTG format] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = (task.memory.mega*0.8).intValue() + "M" + } + + """ + rtg RTG_MEM=${avail_mem} format \\ + ${args} \\ + ${rg} \\ + --output ${prefix}.sdf \\ + ${input} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + def avail_mem = "3G" + if (!task.memory) { + log.info '[RTG format] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' + } else { + avail_mem = (task.memory.mega*0.8).intValue() + "M" + } + """ + touch ${prefix}.sdf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/rtgtools/format/meta.yml b/modules/nf-core/rtgtools/format/meta.yml new file mode 100644 index 0000000..8ed18f5 --- /dev/null +++ b/modules/nf-core/rtgtools/format/meta.yml @@ -0,0 +1,51 @@ +name: "rtgtools_format" +description: Converts the contents of sequence data files (FASTA/FASTQ/SAM/BAM) into the RTG Sequence Data File (SDF) format. +keywords: + - rtg + - fasta + - fastq + - bam + - sam +tools: + - "rtgtools": + description: "RealTimeGenomics Tools -- Utilities for accurate VCF comparison and manipulation" + homepage: "https://www.realtimegenomics.com/products/rtg-tools" + documentation: "https://github.com/RealTimeGenomics/rtg-tools" + tool_dev_url: "https://github.com/RealTimeGenomics/rtg-tools" + licence: "['BSD']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - input1: + type: file + description: FASTA, FASTQ, BAM or SAM file. This should be the left input file when using paired end FASTQ/FASTA data + pattern: "*.{fasta,fa,fna,fastq,fastq.gz,fq,fq.gz,bam,sam}" + - input2: + type: file + description: The right input file when using paired end FASTQ/FASTA data + pattern: "*.{fasta,fa,fna,fastq,fastq.gz,fq,fq.gz}" + - sam_rg: + type: file + description: A file containing a single readgroup header as a SAM header. This can also be supplied as a string in `task.ext.args` as `--sam-rg `. + pattern: "*.{txt,sam}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - sdf: + type: directory + description: The sequence dictionary format folder created from the input file(s) + pattern: "*.sdf" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/modules/nf-core/rtgtools/vcfeval/environment.yml b/modules/nf-core/rtgtools/vcfeval/environment.yml new file mode 100644 index 0000000..e3c6499 --- /dev/null +++ b/modules/nf-core/rtgtools/vcfeval/environment.yml @@ -0,0 +1,7 @@ +name: rtgtools_vcfeval +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::rtg-tools=3.12.1 diff --git a/modules/nf-core/rtgtools/vcfeval/main.nf b/modules/nf-core/rtgtools/vcfeval/main.nf new file mode 100644 index 0000000..98f9adb --- /dev/null +++ b/modules/nf-core/rtgtools/vcfeval/main.nf @@ -0,0 +1,90 @@ +process RTGTOOLS_VCFEVAL { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/rtg-tools:3.12.1--hdfd78af_0': + 'biocontainers/rtg-tools:3.12.1--hdfd78af_0' }" + + input: + tuple val(meta), path(query_vcf), path(query_vcf_tbi), path(truth_vcf), path(truth_vcf_tbi), path(truth_bed), path(evaluation_bed) + tuple val(meta2), path(sdf) + + output: + tuple val(meta), path("*.tp.vcf.gz") , emit: tp_vcf + tuple val(meta), path("*.tp.vcf.gz.tbi") , emit: tp_tbi + tuple val(meta), path("*.fn.vcf.gz") , emit: fn_vcf + tuple val(meta), path("*.fn.vcf.gz.tbi") , emit: fn_tbi + tuple val(meta), path("*.fp.vcf.gz") , emit: fp_vcf + tuple val(meta), path("*.fp.vcf.gz.tbi") , emit: fp_tbi + tuple val(meta), path("*.tp-baseline.vcf.gz") , emit: baseline_vcf + tuple val(meta), path("*.tp-baseline.vcf.gz.tbi") , emit: baseline_tbi + tuple val(meta), path("*.snp_roc.tsv.gz") , emit: snp_roc + tuple val(meta), path("*.non_snp_roc.tsv.gz") , emit: non_snp_roc + tuple val(meta), path("*.weighted_roc.tsv.gz") , emit: weighted_roc + tuple val(meta), path("*.summary.txt") , emit: summary + tuple val(meta), path("*.phasing.txt") , emit: phasing + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: "" + def prefix = task.ext.prefix ?: "${meta.id}" + def bed_regions = truth_bed ? "--bed-regions=${truth_bed}" : "" + def eval_regions = evaluation_bed ? "--evaluation-regions=${evaluation_bed}" : "" + def truth_index = truth_vcf_tbi ? "" : "rtg index ${truth_vcf}" + def query_index = query_vcf_tbi ? "" : "rtg index ${query_vcf}" + def avail_mem = task.memory.toGiga() + "G" + + """ + ${truth_index} + ${query_index} + + rtg RTG_MEM=$avail_mem vcfeval \\ + ${args} \\ + --baseline=${truth_vcf} \\ + ${bed_regions} \\ + ${eval_regions} \\ + --calls=${query_vcf} \\ + --output=output \\ + --template=${sdf} \\ + --threads=${task.cpus} + + cd output/ + mv done progress .. + for f in * ; do mv "\$f" "../${prefix}.\$f" ; done + cd .. + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.tp.vcf.gz + touch ${prefix}.tp.vcf.gz.tbi + touch ${prefix}.fn.vcf.gz + touch ${prefix}.fn.vcf.gz.tbi + touch ${prefix}.fp.vcf.gz + touch ${prefix}.fp.vcf.gz.tbi + touch ${prefix}.tp-baseline.vcf.gz + touch ${prefix}.tp-baseline.vcf.gz.tbi + touch ${prefix}.snp_roc.tsv.gz + touch ${prefix}.non_snp_roc.tsv.gz + touch ${prefix}.weighted_roc.tsv.gz + touch ${prefix}.summary.txt + touch ${prefix}.phasing.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + rtg-tools: \$(echo \$(rtg version | head -n 1 | awk '{print \$4}')) + END_VERSIONS + """ +} diff --git a/modules/nf-core/rtgtools/vcfeval/meta.yml b/modules/nf-core/rtgtools/vcfeval/meta.yml new file mode 100644 index 0000000..f67a35c --- /dev/null +++ b/modules/nf-core/rtgtools/vcfeval/meta.yml @@ -0,0 +1,112 @@ +name: "rtgtools_vcfeval" +description: The VCFeval tool of RTG tools. It is used to evaluate called variants for agreement with a baseline variant set +keywords: + - benchmarking + - vcf + - rtg-tools +tools: + - "rtgtools": + description: "RealTimeGenomics Tools -- Utilities for accurate VCF comparison and manipulation" + homepage: "https://www.realtimegenomics.com/products/rtg-tools" + documentation: "https://github.com/RealTimeGenomics/rtg-tools" + tool_dev_url: "https://github.com/RealTimeGenomics/rtg-tools" + licence: "['BSD']" +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - query_vcf: + type: file + description: A VCF with called variants to benchmark against the standard + pattern: "*.{vcf,vcf.gz}" + - query_vcf_index: + type: file + description: The index of the called VCF (optional) + pattern: "*.tbi" + - truth_vcf: + type: file + description: A standard VCF to compare against + pattern: "*.{vcf,vcf.gz}" + - truth_vcf_index: + type: file + description: The index of the standard VCF (optional) + pattern: "*.tbi" + - truth_bed: + type: file + description: A BED file containining the strict regions where VCFeval should only evaluate the fully overlapping variants (optional) + pattern: "*.bed" + - evaluation_bed: + type: file + description: A BED file containing the regions where VCFeval will evaluate every fully and partially overlapping variant (optional) + pattern: "*.bed" + - sdf: + type: file + description: The SDF (RTG Sequence Data File) folder of the reference genome +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - tp_vcf: + type: file + description: A VCF file for the true positive variants + pattern: "*.tp.vcf.gz" + - tp_tbi: + type: file + description: The index of the VCF file for the true positive variants + pattern: "*.tp.vcf.gz.tbi" + - fn_vcf: + type: file + description: A VCF file for the false negative variants + pattern: "*.fn.vcf.gz" + - fn_tbi: + type: file + description: The index of the VCF file for the false negative variants + pattern: "*.fn.vcf.gz.tbi" + - fp_vcf: + type: file + description: A VCF file for the false positive variants + pattern: "*.fp.vcf.gz" + - fp_tbi: + type: file + description: The index of the VCF file for the false positive variants + pattern: "*.fp.vcf.gz.tbi" + - baseline_vcf: + type: file + description: A VCF file for the true positive variants from the baseline + pattern: "*.tp-baseline.vcf.gz" + - baseline_tbi: + type: file + description: The index of the VCF file for the true positive variants from the baseline + pattern: "*.tp-baseline.vcf.gz.tbi" + - snp_roc: + type: file + description: TSV files containing ROC data for the SNPs + pattern: "*.snp_roc.tsv.gz" + - non_snp_roc: + type: file + description: TSV files containing ROC data for all variants except SNPs + pattern: "*.non_snp_roc.tsv.gz" + - weighted_roc: + type: file + description: TSV files containing weighted ROC data for all variants + pattern: "*.weighted_snp_roc.tsv.gz" + - summary: + type: file + description: A TXT file containing the summary of the evaluation + pattern: "*.summary.txt" + - phasing: + type: file + description: A TXT file containing the data on the phasing + pattern: "*.phasing.txt" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index ad04130..e6a04b0 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -27,9 +27,6 @@ workflow PREPARE_VCFS_TEST { versions=Channel.empty() - //ref.map { it -> tuple([id: it[0].baseName], it[1]) } - // .set{fasta} - // // MODULE: BGZIP_TABIX // @@ -94,6 +91,7 @@ workflow PREPARE_VCFS_TEST { BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) .set{vcf_ch} } + // TODO: this part should onyl run for SV bench if (params.min_sv_size > 0){ TABIX_BGZIP( diff --git a/subworkflows/local/small_germline_benchmark.nf b/subworkflows/local/small_germline_benchmark.nf new file mode 100644 index 0000000..f585707 --- /dev/null +++ b/subworkflows/local/small_germline_benchmark.nf @@ -0,0 +1,54 @@ +// +// SMALL_GERMLINE_BENCHMARK: SUBWORKFLOW FOR SMALL GERMLINE VARIANTS +// + +params.options = [:] + +include { RTGTOOLS_FORMAT } from '../../modules/nf-core/rtgtools/format/main' addParams( options: params.options ) +include { RTGTOOLS_VCFEVAL } from '../../modules/nf-core/rtgtools/vcfeval/main' addParams( options: params.options ) +include { HAPPY_HAPPY } from '../../modules/nf-core/happy/happy/main' addParams( options: params.options ) + +workflow SMALL_GERMLINE_BENCHMARK { + take: + input_ch // channel: [val(meta),test_vcf,test_index,truth_vcf,truth_index, bed] + ref // reference channel [ref.fa, ref.fa.fai] + + main: + + versions=Channel.empty() + + if (params.method.contains('rtgtools')){ + // + // MODULE: RTGTOOLS_FORMAT + // + RTGTOOLS_FORMAT( + ref.map { it -> tuple([id: it[0].getSimpleName(), "pair": "single_end"], it[0], [], []) } + ) + versions = versions.mix(RTGTOOLS_FORMAT.out.versions) + + // + // MODULE: RTGTOOLS_VCFEVAL + // + RTGTOOLS_VCFEVAL( + input_ch.map { it -> tuple(it[0],it[3], it[4], it[1], it[2], it[5], []) }, + RTGTOOLS_FORMAT.out.sdf + ) + versions = versions.mix(RTGTOOLS_VCFEVAL.out.versions) + } + + if (params.method.contains('happy')){ + + HAPPY_HAPPY( + input_ch.map { it -> tuple(it[0],it[3], it[1], it[5], []) }, + ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) }, + ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) }, + [[],[]], + [[],[]], + [[],[]] + ) + versions = versions.mix(HAPPY_HAPPY.out.versions) + } + + emit: + versions +} diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index c3c9be0..733d827 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -25,6 +25,7 @@ include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pi include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' include { SOMATIC_BENCHMARK } from '../subworkflows/local/somatic_benchmark' include { SV_GERMLINE_BENCHMARK } from '../subworkflows/local/sv_germline_benchmark' +include { SMALL_GERMLINE_BENCHMARK } from '../subworkflows/local/small_germline_benchmark' include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' include { SV_VCF_CONVERSIONS } from '../subworkflows/local/sv_vcf_conversion' @@ -140,6 +141,16 @@ workflow VARIANTBENCHMARKING { cnv: it[0].vartype == "cnv" other: false} .set{input} + // + // SUBWORKFLOW: SMALL_GERMLINE_BENCHMARK + // + //Benchmarking spesific to germline samples + + SMALL_GERMLINE_BENCHMARK( + input.small, + ref ) + ch_versions = ch_versions.mix(SMALL_GERMLINE_BENCHMARK.out.versions) + // // SUBWORKFLOW: SV_GERMLINE_BENCHMARK From d79ecbb3b8be5f1749fc7b6584cfc0ed4556e083 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 13 Mar 2024 12:18:41 +0000 Subject: [PATCH 039/292] ref changed to fasta and fai --- .gitignore | 1 + modules/local/bamsurgeon_evaluator.nf | 9 ++++---- modules/local/gridss_annotation.nf | 3 ++- modules/local/truvari_phab.nf | 9 ++++---- modules/local/truvari_refine.nf | 5 +++-- modules/local/vcf_genotype_annotator.nf | 6 ++--- modules/local/vcfdist.nf | 3 ++- subworkflows/local/prepare_vcfs_test.nf | 9 ++++---- subworkflows/local/prepare_vcfs_truth.nf | 9 ++++---- .../local/small_germline_benchmark.nf | 9 ++++---- subworkflows/local/somatic_benchmark.nf | 6 +++-- subworkflows/local/sv_germline_benchmark.nf | 22 ++++++++++--------- subworkflows/local/sv_vcf_conversion.nf | 8 ++++--- workflows/variantbenchmarking.nf | 22 +++++++++++++------ 14 files changed, 72 insertions(+), 49 deletions(-) diff --git a/.gitignore b/.gitignore index 5124c9a..fdab1ac 100644 --- a/.gitignore +++ b/.gitignore @@ -6,3 +6,4 @@ results/ testing/ testing* *.pyc +test_small*/ diff --git a/modules/local/bamsurgeon_evaluator.nf b/modules/local/bamsurgeon_evaluator.nf index 384a515..f903cc2 100644 --- a/modules/local/bamsurgeon_evaluator.nf +++ b/modules/local/bamsurgeon_evaluator.nf @@ -8,13 +8,14 @@ process BAMSURGEON_EVALUATOR { 'lethalfang/bamsurgeon:1.2' }" input: - tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi) - tuple path(fasta), path(fai) + tuple val(meta), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi) + tuple val(meta2), path(fasta), + tuple val(meta3), path(fai) val(muttype) output: - tuple val(meta),val(meta2), path("*.{vcf}"), emit: bench - path "versions.yml" , emit: versions + tuple val(meta),path("*.{vcf}"), emit: bench + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/local/gridss_annotation.nf b/modules/local/gridss_annotation.nf index 4e0a090..7be0a5b 100644 --- a/modules/local/gridss_annotation.nf +++ b/modules/local/gridss_annotation.nf @@ -10,7 +10,8 @@ process GRIDSS_ANNOTATION { input: tuple val(meta), path(vcf), path(index) - tuple path(fasta), path(fasta_fai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fasta_fai) output: tuple val(meta),path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf diff --git a/modules/local/truvari_phab.nf b/modules/local/truvari_phab.nf index ea65b3a..0f032ff 100644 --- a/modules/local/truvari_phab.nf +++ b/modules/local/truvari_phab.nf @@ -8,12 +8,13 @@ process TRUVARI_PHAB { 'kubran/truvari:v4.3.0' }" input: - tuple val(meta),val(meta2), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) - tuple path(fasta), path(fai) + tuple val(meta), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) output: - tuple val(meta), val(meta2), path("*.vcf.gz") , emit: harmon - path "versions.yml" , emit: versions + tuple val(meta),path("*.vcf.gz") , emit: harmon + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/local/truvari_refine.nf b/modules/local/truvari_refine.nf index 517480d..a3748f0 100644 --- a/modules/local/truvari_refine.nf +++ b/modules/local/truvari_refine.nf @@ -8,9 +8,10 @@ process TRUVARI_REFINE { 'kubran/truvari:v4.3.0' }" input: - tuple val(meta), val(meta2), path(bench) + tuple val(meta), path(bench) each path(bed) - tuple path(fasta), path(fai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) output: path "versions.yml" , emit: versions diff --git a/modules/local/vcf_genotype_annotator.nf b/modules/local/vcf_genotype_annotator.nf index cd59090..1931b8a 100644 --- a/modules/local/vcf_genotype_annotator.nf +++ b/modules/local/vcf_genotype_annotator.nf @@ -8,11 +8,11 @@ process VCF_GENOTYPE_ANNOTATOR { 'griffithlab/vatools:5.1.10' }" input: - tuple val(meta),val(meta2), path(vcf) + tuple val(meta),path(vcf) output: - tuple val(meta),val(meta2), path("*.{vcf}"), emit: vcf - path "versions.yml" , emit: versions + tuple val(meta),path("*.{vcf}"), emit: vcf + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/local/vcfdist.nf b/modules/local/vcfdist.nf index a2771c7..2149f72 100644 --- a/modules/local/vcfdist.nf +++ b/modules/local/vcfdist.nf @@ -9,7 +9,8 @@ process VCFDIST { input: tuple val(meta),path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) - tuple path(fasta), path(fai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) output: tuple val(meta), path("*.tsv,vcf"), emit: bench diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index e6a04b0..4b21ba4 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -21,7 +21,8 @@ include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TEST } from '../../modules/nf-c workflow PREPARE_VCFS_TEST { take: input_ch // channel: [val(meta),vcf] - ref // reference channel [ref.fa, ref.fa.fai] + fasta // reference channel [val(meta), ref.fa] + fai // reference channel [val(meta), ref.fa.fai] main: @@ -50,7 +51,7 @@ workflow PREPARE_VCFS_TEST { BCFTOOLS_REHEADER_TEST( input_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } + fai ) versions = versions.mix(BCFTOOLS_REHEADER_TEST.out.versions) @@ -80,7 +81,7 @@ workflow PREPARE_VCFS_TEST { // Breaks down -any- multi-allelic variants BCFTOOLS_NORM_1( vcf_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } + fasta ) versions = versions.mix(BCFTOOLS_NORM_1.out.versions) @@ -125,7 +126,7 @@ workflow PREPARE_VCFS_TEST { // Deduplicates variants at the same position test BCFTOOLS_NORM_2( vcf_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } + fasta ) versions = versions.mix(BCFTOOLS_NORM_2.out.versions) diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 87e2489..7354727 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -17,7 +17,8 @@ include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TRUTH } from '../../modules/nf- workflow PREPARE_VCFS_TRUTH { take: truth_ch // channel: [val(meta), vcf] - ref // reference channel [ref.fa, ref.fa.fai] + fasta // reference channel [val(meta), ref.fa] + fai // reference channel [val(meta), ref.fa.fai] main: @@ -46,7 +47,7 @@ workflow PREPARE_VCFS_TRUTH { BCFTOOLS_REHEADER_TRUTH( input_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } + fai ) versions = versions.mix(BCFTOOLS_REHEADER_TRUTH.out.versions) @@ -63,7 +64,7 @@ workflow PREPARE_VCFS_TRUTH { // multi-allelic variants will be splitted. BCFTOOLS_NORM_1( vcf_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } + fasta ) versions = versions.mix(BCFTOOLS_NORM_1.out.versions) @@ -82,7 +83,7 @@ workflow PREPARE_VCFS_TRUTH { // Deduplicate variants at the same position BCFTOOLS_NORM_2( vcf_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } + fasta ) versions = versions.mix(BCFTOOLS_NORM_2.out.versions) diff --git a/subworkflows/local/small_germline_benchmark.nf b/subworkflows/local/small_germline_benchmark.nf index f585707..3f1439c 100644 --- a/subworkflows/local/small_germline_benchmark.nf +++ b/subworkflows/local/small_germline_benchmark.nf @@ -11,7 +11,8 @@ include { HAPPY_HAPPY } from '../../modules/nf-core/happy/happy/main' workflow SMALL_GERMLINE_BENCHMARK { take: input_ch // channel: [val(meta),test_vcf,test_index,truth_vcf,truth_index, bed] - ref // reference channel [ref.fa, ref.fa.fai] + fasta // reference channel [val(meta), ref.fa] + fai // reference channel [val(meta), ref.fa.fai] main: @@ -22,7 +23,7 @@ workflow SMALL_GERMLINE_BENCHMARK { // MODULE: RTGTOOLS_FORMAT // RTGTOOLS_FORMAT( - ref.map { it -> tuple([id: it[0].getSimpleName(), "pair": "single_end"], it[0], [], []) } + fasta.map { it -> tuple([id: it[1].getSimpleName(), "pair": "single_end"], it[1], [], []) } ) versions = versions.mix(RTGTOOLS_FORMAT.out.versions) @@ -40,8 +41,8 @@ workflow SMALL_GERMLINE_BENCHMARK { HAPPY_HAPPY( input_ch.map { it -> tuple(it[0],it[3], it[1], it[5], []) }, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) }, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) }, + fasta, + fai, [[],[]], [[],[]], [[],[]] diff --git a/subworkflows/local/somatic_benchmark.nf b/subworkflows/local/somatic_benchmark.nf index cc1bc80..b951066 100644 --- a/subworkflows/local/somatic_benchmark.nf +++ b/subworkflows/local/somatic_benchmark.nf @@ -10,7 +10,8 @@ include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svan workflow SOMATIC_BENCHMARK { take: input_ch // channel: [val(meta), test_vcf,test_index, truth_vcf, truth_index, bed] - ref // reference channel [ref.fa, ref.fa.fai] + fasta // reference channel [val(meta), ref.fa] + fai // reference channel [val(meta), ref.fa.fai] main: @@ -22,7 +23,8 @@ workflow SOMATIC_BENCHMARK { // TRUVARI_BENCH( input_ch, - ref + fasta, + fai ) versions = versions.mix(TRUVARI_BENCH.out.versions) diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index 75be720..58783e0 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -14,7 +14,8 @@ include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon_evaluato workflow SV_GERMLINE_BENCHMARK { take: input_ch // channel: [val(meta),test_vcf,test_index,truth_vcf,truth_index, bed] - ref // reference channel [ref.fa, ref.fa.fai] + fasta // reference channel [val(meta), ref.fa] + fai // reference channel [val(meta), ref.fa.fai] main: @@ -30,7 +31,8 @@ workflow SV_GERMLINE_BENCHMARK { // TRUVARI_PHAB( input_ch, - ref + fasta, + fai ) } // @@ -38,8 +40,8 @@ workflow SV_GERMLINE_BENCHMARK { // TRUVARI_BENCH( input_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) }, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } + fasta, + fai ) versions = versions.mix(TRUVARI_BENCH.out.versions) @@ -52,8 +54,8 @@ workflow SV_GERMLINE_BENCHMARK { // slower than truvari SVANALYZER_SVBENCHMARK( input_ch, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) }, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[1]) } + fasta, + fai ) versions = versions.mix(SVANALYZER_SVBENCHMARK.out.versions) @@ -78,7 +80,8 @@ workflow SV_GERMLINE_BENCHMARK { // VCFDIST( input_ch, - ref + fasta, + fai ) versions = versions.mix(VCFDIST.out.versions) } @@ -90,14 +93,13 @@ workflow SV_GERMLINE_BENCHMARK { //https://github.com/adamewing/bamsurgeon/blob/master/scripts/evaluator.py BAMSURGEON_EVALUATOR( input_ch.map{it -> tuple(it[0],it[1], it[2], it[3], it[4], it[5])}, - ref, + fasta, + fai, "SV" ) versions = versions.mix(BAMSURGEON_EVALUATOR.out.versions) } - - emit: versions } diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 09ab7c9..3f2988b 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -12,7 +12,8 @@ include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' workflow SV_VCF_CONVERSIONS { take: input_ch // channel: [val(meta), vcf] - ref // reference channel [ref.fa, ref.fa.fai] + fasta // reference channel [val(meta), ref.fa] + fai // reference channel [val(meta), ref.fa.fai] svync_yaml // yaml configs main: @@ -73,7 +74,7 @@ workflow SV_VCF_CONVERSIONS { MANTA_CONVERTINVERSION( input.tool.map{it -> tuple(it[0], it[1])}, - ref.map { it -> tuple([id: it[0].getSimpleName()], it[0]) } + fasta ) versions = versions.mix(MANTA_CONVERTINVERSION.out.versions) @@ -101,7 +102,8 @@ workflow SV_VCF_CONVERSIONS { // GRIDSS simple event annotation GRIDSS_ANNOTATION( input.tool, - ref + fasta, + fai ) versions = versions.mix(GRIDSS_ANNOTATION.out.versions) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 733d827..98d623f 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -51,7 +51,9 @@ workflow VARIANTBENCHMARKING { // check mandatory parameters println(params.fasta) println(params.fai) - ref = Channel.fromPath([params.fasta,params.fai], checkIfExists: true).collect() + + fasta = Channel.fromPath(params.fasta, checkIfExists: true).map{ it -> tuple([id: it[0].getSimpleName()], it) }.collect() + fai = Channel.fromPath(params.fai, checkIfExists: true).map{ it -> tuple([id: it[0].getSimpleName()], it) }.collect() // check high confidence files @@ -82,7 +84,8 @@ workflow VARIANTBENCHMARKING { // Standardize SV VCFs, tool spesific modifications SV_VCF_CONVERSIONS( input.sv, - ref, + fasta, + fai, svync_yaml ) ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) @@ -95,13 +98,15 @@ workflow VARIANTBENCHMARKING { // PREPARE_VCFS_TEST( out_vcf_ch, - ref + fasta, + fai ) ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) PREPARE_VCFS_TRUTH( truth, - ref + fasta, + fai ) ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) @@ -148,7 +153,8 @@ workflow VARIANTBENCHMARKING { SMALL_GERMLINE_BENCHMARK( input.small, - ref ) + fasta, + fai ) ch_versions = ch_versions.mix(SMALL_GERMLINE_BENCHMARK.out.versions) @@ -159,7 +165,8 @@ workflow VARIANTBENCHMARKING { SV_GERMLINE_BENCHMARK( input.sv, - ref ) + fasta, + fai ) ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) // TODO: SOMATIC EBNCHMARKING @@ -168,7 +175,8 @@ workflow VARIANTBENCHMARKING { // SOMATIC VARIANT BENCHMARKING SOMATIC_BENCHMARK( bench_ch, - ref + fasta, + fai ) ch_versions = ch_versions.mix(SOMATIC_BENCHMARK.out.versions) } From b8f19394fab404947eeab4fc315a48b7186802bf Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 13 Mar 2024 16:24:00 +0000 Subject: [PATCH 040/292] happy-prepy added --- conf/modules.config | 9 ++ conf/test.config | 2 +- conf/test_hg19.config | 4 +- conf/test_hg37.config | 4 +- conf/test_hg38.config | 4 +- conf/test_hg38_somatic.config | 6 +- conf/test_small.config | 6 +- modules.json | 5 + modules/nf-core/happy/prepy/environment.yml | 7 ++ modules/nf-core/happy/prepy/main.nf | 56 +++++++++ modules/nf-core/happy/prepy/meta.yml | 63 ++++++++++ .../nf-core/happy/prepy/tests/main.nf.test | 113 ++++++++++++++++++ .../happy/prepy/tests/main.nf.test.snap | 93 ++++++++++++++ modules/nf-core/happy/prepy/tests/tags.yml | 2 + subworkflows/local/prepare_vcfs_test.nf | 19 +++ subworkflows/local/prepare_vcfs_truth.nf | 4 +- workflows/variantbenchmarking.nf | 39 ++++-- 17 files changed, 411 insertions(+), 25 deletions(-) create mode 100644 modules/nf-core/happy/prepy/environment.yml create mode 100644 modules/nf-core/happy/prepy/main.nf create mode 100644 modules/nf-core/happy/prepy/meta.yml create mode 100644 modules/nf-core/happy/prepy/tests/main.nf.test create mode 100644 modules/nf-core/happy/prepy/tests/main.nf.test.snap create mode 100644 modules/nf-core/happy/prepy/tests/tags.yml diff --git a/conf/modules.config b/conf/modules.config index df98a46..215fc02 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -131,6 +131,15 @@ process { mode: params.publish_dir_mode ] } + withName: "HAPPY_PREPY" { + ext.prefix = {"${params.sample}_prepy"} + ext.args = {"--fixchr --filter-nonref --bcftools-norm"} + publishDir = [ + path: {"${params.outdir}/${meta.id}/preprocess"}, + pattern: "*{vcf.gz}", + mode: params.publish_dir_mode + ] + } withName: "TRUVARI_BENCH" { ext.prefix = {"${params.sample}"} ext.args = {"--pctsize 0.5 --pctovl 0.5 --refdist 1000 --pick ac"} diff --git a/conf/test.config b/conf/test.config index 35a66c1..cb4b3c9 100644 --- a/conf/test.config +++ b/conf/test.config @@ -34,5 +34,5 @@ params { min_sv_size = 50 standardization = true - truth = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" } diff --git a/conf/test_hg19.config b/conf/test_hg19.config index 3623854..d7a89f6 100644 --- a/conf/test_hg19.config +++ b/conf/test_hg19.config @@ -36,7 +36,7 @@ params { //bnd_to_inv = true sample = "HG002" // available samples: SEQC2, HG002 - truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.vcf.gz" - high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.bed" + truth_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.vcf.gz" + high_conf_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.bed" } diff --git a/conf/test_hg37.config b/conf/test_hg37.config index aa20eb1..d47de30 100644 --- a/conf/test_hg37.config +++ b/conf/test_hg37.config @@ -36,8 +36,8 @@ params { //bnd_to_inv = true sample = "HG002" // available samples: SEQC2, HG002 - truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.vcf.gz" - high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.bed" + truth_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.vcf.gz" + high_conf_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.bed" } diff --git a/conf/test_hg38.config b/conf/test_hg38.config index afb8c82..aaeb3fd 100644 --- a/conf/test_hg38.config +++ b/conf/test_hg38.config @@ -48,7 +48,7 @@ params { //standardization = true sample = "HG002" // available samples: SEQC2, HG002 - truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.vcf.gz" - high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.bed" + truth_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.vcf.gz" + high_conf_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.bed" } diff --git a/conf/test_hg38_somatic.config b/conf/test_hg38_somatic.config index e8abecc..b4354ae 100644 --- a/conf/test_hg38_somatic.config +++ b/conf/test_hg38_somatic.config @@ -32,8 +32,8 @@ params { sample = "SEQC2" // available samples: SEQC2, HG002 - truth = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/sSNV_truth_set_v1.0.vcf.gz" - high_conf = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/High-Confidence_Regions_v1.2.bed" - rename_chromosomes = "assets/rename_chroms_hg38.txt" + truth_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/sSNV_truth_set_v1.0.vcf.gz" + high_conf_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/SEQC_somatic_mutation_truth/High-Confidence_Regions_v1.2.bed" + //rename_chromosomes = "assets/rename_chroms_hg38.txt" } diff --git a/conf/test_small.config b/conf/test_small.config index 8c505eb..b998525 100644 --- a/conf/test_small.config +++ b/conf/test_small.config @@ -29,8 +29,8 @@ params { // Processes analysis = 'germline' method = 'rtgtools,happy' // - preprocess = "normalization, deduplication" - //min_sv_size = 50 + preprocess = "normalization,deduplication,prepy" + //min_sv_size = 50 - truth = "https://raw.githubusercontent.com/nf-core/test-datasets/modules//data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz" + truth_small = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz" } diff --git a/modules.json b/modules.json index 5822726..78603d7 100644 --- a/modules.json +++ b/modules.json @@ -25,6 +25,11 @@ "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] }, + "happy/prepy": { + "branch": "master", + "git_sha": "01b55f288c25490236af1cd044c9bca17598ecfe", + "installed_by": ["modules"] + }, "manta/convertinversion": { "branch": "master", "git_sha": "1e2b7fb7106852388610c0360d234b0829eb980e", diff --git a/modules/nf-core/happy/prepy/environment.yml b/modules/nf-core/happy/prepy/environment.yml new file mode 100644 index 0000000..d159834 --- /dev/null +++ b/modules/nf-core/happy/prepy/environment.yml @@ -0,0 +1,7 @@ +name: happy_prepy +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::hap.py=0.3.14 diff --git a/modules/nf-core/happy/prepy/main.nf b/modules/nf-core/happy/prepy/main.nf new file mode 100644 index 0000000..57333ac --- /dev/null +++ b/modules/nf-core/happy/prepy/main.nf @@ -0,0 +1,56 @@ +process HAPPY_PREPY { + tag "$meta.id" + label 'process_medium' + + // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/hap.py:0.3.14--py27h5c5a3ab_0': + 'biocontainers/hap.py:0.3.14--py27h5c5a3ab_0' }" + + input: + tuple val(meta), path(vcf), path(bed) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fasta_fai) + + output: + tuple val(meta), path('*.vcf.gz') , emit: preprocessed_vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def restrict_region = bed ? "-R ${bed}": "" + def VERSION = '0.3.14' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + pre.py \\ + $args \\ + $restrict_region \\ + --reference $fasta \\ + --threads $task.cpus \\ + $vcf \\ + ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pre.py: $VERSION + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def restrict_region = bed ? "-R ${bed}": "" + def VERSION = '0.3.14' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + echo "" | gzip > ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + pre.py: $VERSION + END_VERSIONS + """ +} diff --git a/modules/nf-core/happy/prepy/meta.yml b/modules/nf-core/happy/prepy/meta.yml new file mode 100644 index 0000000..3e8898f --- /dev/null +++ b/modules/nf-core/happy/prepy/meta.yml @@ -0,0 +1,63 @@ +name: "happy_prepy" +description: Pre.py is a preprocessing tool made to preprocess VCF files for Hap.py +keywords: + - happy + - benchmark + - haplotype +tools: + - "happy": + description: Haplotype VCF comparison tools + homepage: https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub/apps/hap-py-benchmarking.html + documentation: https://github.com/Illumina/hap.py + tool_dev_url: https://github.com/Illumina/hap.py + licence: ["BSD-2-clause"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - meta2: + type: map + description: | + Groovy Map containing sample information for reference fasta + e.g. [ id:'test2'] + - meta3: + type: map + description: | + Groovy Map containing sample information for reference fai + e.g. [ id:'test3' ] + - vcf: + type: file + description: VCF file to preprocess + pattern: "*.{vcf,vcf.gz}" + - bed: + type: file + description: BED file + pattern: "*.bed" + - fasta: + type: file + description: FASTA file of the reference genome + pattern: "*.{fa,fasta}" + - fasta_fai: + type: file + description: The index of the reference FASTA + pattern: "*.fai" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: A preprocessed VCF file + pattern: "*.vcf.gz" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/modules/nf-core/happy/prepy/tests/main.nf.test b/modules/nf-core/happy/prepy/tests/main.nf.test new file mode 100644 index 0000000..1146997 --- /dev/null +++ b/modules/nf-core/happy/prepy/tests/main.nf.test @@ -0,0 +1,113 @@ +nextflow_process { + + name "Test Process HAPPY_PREPY" + script "../main.nf" + process "HAPPY_PREPY" + + tag "modules" + tag "modules_nfcore" + tag "happy" + tag "happy/prepy" + + test("homo_sapiens - illumina - vcf - bed") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true) + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.preprocessed_vcf[0].collect { it instanceof Map ? it : path(it).linesGzip[-3..-1] } + ).match("bed_preprocessed_vcf") }, + { assert snapshot(process.out.versions).match("bed_versions") } + ) + } + + } + test("homo_sapiens - illumina - vcf") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true), + [] + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.preprocessed_vcf[0].collect { it instanceof Map ? it : path(it).linesGzip[-3..-1] } + ).match("preprocessed_vcf") }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_test2_paired_mutect2_calls_vcf_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true) + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.test_data['homo_sapiens']['genome']['genome_21_fasta_fai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.preprocessed_vcf).match("stub_preprocessed_vcf") }, + { assert snapshot(process.out.versions).match("stub_versions") } + ) + } + + } + +} diff --git a/modules/nf-core/happy/prepy/tests/main.nf.test.snap b/modules/nf-core/happy/prepy/tests/main.nf.test.snap new file mode 100644 index 0000000..f26077d --- /dev/null +++ b/modules/nf-core/happy/prepy/tests/main.nf.test.snap @@ -0,0 +1,93 @@ +{ + "bed_preprocessed_vcf": { + "content": [ + [ + { + "id": "test" + }, + [ + "chr21\t45184948\t.\tT\tA\t0\t.\tAS_SB_TABLE=19,1|2,0;DP=23;ECNT=1;MBQ=15,18;MFRL=394,448;MMQ=60,60;MPOS=45;NALOD=1.08;NLOD=3.26;POPAF=6;TLOD=5.22\tGT:AD:ADO:DP:F1R2:F2R1:FAD:SB:AF\t0/0:.,.:.:11:.:.:.:.:.\t0/1:9,2:0:11:0:0:2:0:0.25", + "chr21\t45984818\t.\tA\tT\t0\t.\tAS_SB_TABLE=0,16|0,2;DP=19;ECNT=1;MBQ=15,17;MFRL=439,502;MMQ=60,60;MPOS=28;NALOD=1.05;NLOD=2.83;POPAF=6;TLOD=5.36\tGT:AD:ADO:DP:F1R2:F2R1:FAD:SB:AF\t0/0:.,.:.:10:.:.:.:.:.\t0/1:6,2:0:8:0:0:2:2:0.309", + "chr21\t46335080\t.\tA\tT\t0\t.\tAS_SB_TABLE=0,37|0,4;DP=44;ECNT=1;MBQ=23,18;MFRL=461,571;MMQ=60,60;MPOS=30;NALOD=1.35;NLOD=6.31;POPAF=6;TLOD=3.02\tGT:AD:ADO:DP:F1R2:F2R1:FAD:SB:AF\t0/0:.,.:.:21:.:.:.:.:.\t0/1:16,4:0:20:0:0:4:4:0.209" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-13T15:12:58.950764084" + }, + "preprocessed_vcf": { + "content": [ + [ + { + "id": "test" + }, + [ + "chr21\t45184948\t.\tT\tA\t0\t.\tAS_SB_TABLE=19,1|2,0;DP=23;ECNT=1;MBQ=15,18;MFRL=394,448;MMQ=60,60;MPOS=45;NALOD=1.08;NLOD=3.26;POPAF=6;TLOD=5.22\tGT:AD:ADO:DP:F1R2:F2R1:FAD:SB:AF\t0/0:.,.:.:11:.:.:.:.:.\t0/1:9,2:0:11:0:0:2:0:0.25", + "chr21\t45984818\t.\tA\tT\t0\t.\tAS_SB_TABLE=0,16|0,2;DP=19;ECNT=1;MBQ=15,17;MFRL=439,502;MMQ=60,60;MPOS=28;NALOD=1.05;NLOD=2.83;POPAF=6;TLOD=5.36\tGT:AD:ADO:DP:F1R2:F2R1:FAD:SB:AF\t0/0:.,.:.:10:.:.:.:.:.\t0/1:6,2:0:8:0:0:2:2:0.309", + "chr21\t46335080\t.\tA\tT\t0\t.\tAS_SB_TABLE=0,37|0,4;DP=44;ECNT=1;MBQ=23,18;MFRL=461,571;MMQ=60,60;MPOS=30;NALOD=1.35;NLOD=6.31;POPAF=6;TLOD=3.02\tGT:AD:ADO:DP:F1R2:F2R1:FAD:SB:AF\t0/0:.,.:.:21:.:.:.:.:.\t0/1:16,4:0:20:0:0:4:4:0.209" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-13T15:13:11.548146422" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,0a8f8cf051042e072869877cbcabcb7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-13T13:57:40.194669566" + }, + "bed_versions": { + "content": [ + [ + "versions.yml:md5,0a8f8cf051042e072869877cbcabcb7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-13T14:54:02.152541068" + }, + "stub_preprocessed_vcf": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-13T15:15:23.105497676" + }, + "stub_versions": { + "content": [ + [ + "versions.yml:md5,0a8f8cf051042e072869877cbcabcb7d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-13T14:54:26.061357048" + } +} \ No newline at end of file diff --git a/modules/nf-core/happy/prepy/tests/tags.yml b/modules/nf-core/happy/prepy/tests/tags.yml new file mode 100644 index 0000000..bb6051f --- /dev/null +++ b/modules/nf-core/happy/prepy/tests/tags.yml @@ -0,0 +1,2 @@ +happy/prepy: + - "modules/nf-core/happy/prepy/**" diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 4b21ba4..73563fa 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -8,10 +8,12 @@ include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' ad include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) +include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' addParams( options: params.options ) include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { TABIX_TABIX as TABIX_TABIX_3 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_1 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_2 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_3 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) @@ -137,6 +139,23 @@ workflow PREPARE_VCFS_TEST { BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) .set{vcf_ch} } + // only for small variant benchmarking + if (params.preprocess.contains("prepy")){ + HAPPY_PREPY( + vcf_ch.map{it -> tuple( it[0], it[1],[])}, + fasta, + fai + ) + // TODO: Check norm settings https://github.com/Illumina/hap.py/blob/master/doc/normalisation.md + + TABIX_TABIX_3( + HAPPY_PREPY.out.preprocessed_vcf + ) + + HAPPY_PREPY.out.preprocessed_vcf.join(TABIX_TABIX_3.out.tbi, by:0) + .set{vcf_ch} + } + emit: vcf_ch versions diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 7354727..223e468 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -27,12 +27,10 @@ workflow PREPARE_VCFS_TRUTH { // // PREPARE_VCFS // - truth_ch.map { it -> tuple([id: "truth"], it[0]) } - .set{truth} // BGZIP if needed and index truth BGZIP_TABIX( - truth + truth_ch ) versions = versions.mix(BGZIP_TABIX.out.versions) vcf_ch = BGZIP_TABIX.out.gz_tbi diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 98d623f..fde91b4 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -47,6 +47,8 @@ workflow VARIANTBENCHMARKING { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() + truth_ch = Channel.empty() + high_conf_ch = Channel.empty() // check mandatory parameters println(params.fasta) @@ -57,14 +59,32 @@ workflow VARIANTBENCHMARKING { // check high confidence files - truth = params.truth ? Channel.fromPath(params.truth, checkIfExists: true).collect() - : Channel.empty() + truth_small = params.truth_small ? Channel.fromPath(params.truth_small, checkIfExists: true).map{ it -> tuple([id: params.sample, vartype:"small"], it) }.collect() + : Channel.empty() + truth_ch = truth_ch.mix(truth_small) - high_conf = params.high_conf ? Channel.fromPath(params.high_conf, checkIfExists: true).collect() - : Channel.empty() + high_conf_small = params.high_conf_small ? Channel.fromPath(params.high_conf_small, checkIfExists: true).map{ it -> tuple([id: params.sample, vartype:"small"], it) }.collect() + : Channel.empty() + high_conf_ch = high_conf_ch.mix(high_conf_small) - svync_yaml = params.standardization ? Channel.fromPath("assets/svync/*.yaml").collect() - : Channel.empty() + truth_sv = params.truth_sv ? Channel.fromPath(params.truth_sv, checkIfExists: true).map{ it -> tuple([id: params.sample, vartype:"sv"], it) }.collect() + : Channel.empty() + truth_ch = truth_ch.mix(truth_sv) + + high_conf_sv = params.high_conf_sv ? Channel.fromPath(params.high_conf_sv, checkIfExists: true).map{ it -> tuple([id: params.sample, vartype:"sv"], it) }.collect() + : Channel.empty() + high_conf_ch = high_conf_ch.mix(high_conf_sv) + + truth_cnv = params.truth_cnv ? Channel.fromPath(params.truth_cnv, checkIfExists: true).map{ it -> tuple([id: params.sample, vartype:"cnv"], it) }.collect() + : Channel.empty() + truth_ch = truth_ch.mix(truth_cnv) + + high_conf_cnv = params.high_conf_cnv ? Channel.fromPath(params.high_conf_cnv, checkIfExists: true).map{ it -> tuple([id: params.sample, vartype:"cnv"], it) }.collect() + : Channel.empty() + high_conf_ch = high_conf_ch.mix(high_conf_cnv) + + svync_yaml = params.standardization ? Channel.fromPath("assets/svync/*.yaml").collect() + : Channel.empty() // TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID @@ -104,7 +124,7 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) PREPARE_VCFS_TRUTH( - truth, + truth_ch, fasta, fai ) @@ -128,9 +148,9 @@ workflow VARIANTBENCHMARKING { ch_versions = ch_versions.mix(REPORT_STATISTICS_TRUTH.out.versions) // prepare benchmark set - if (params.high_conf){ + if (params.high_conf_small || params.high_conf_sv || params.high_conf_cnv ){ PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) - .combine(high_conf) + .combine(high_conf_ch) .set{bench_ch} }else{ PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) @@ -215,6 +235,7 @@ workflow VARIANTBENCHMARKING { ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) + MULTIQC ( ch_multiqc_files.collect(), ch_multiqc_config.toList(), From 276953eb78d2f8e6ca7f66dd6392af733e282c6f Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 14 Mar 2024 16:15:44 +0000 Subject: [PATCH 041/292] fix test-truth statistics --- .gitignore | 2 + assets/samplesheet_small.csv | 2 +- conf/test.config | 3 +- conf/test_small.config | 3 +- nextflow.config | 20 ++--- nextflow_schema.json | 74 ++++++++++++------- subworkflows/local/prepare_vcfs_test.nf | 64 +++++++++++----- subworkflows/local/prepare_vcfs_truth.nf | 1 - .../local/report_benchmark_statistics.nf | 28 +++++++ subworkflows/local/report_statistics_truth.nf | 51 ------------- ...stics_test.nf => report_vcf_statistics.nf} | 4 +- ...enchmark.nf => small_somatic_benchmark.nf} | 4 +- subworkflows/local/sv_vcf_conversion.nf | 6 +- subworkflows/local/visualizations.nf | 35 --------- workflows/variantbenchmarking.nf | 19 ++--- 15 files changed, 146 insertions(+), 170 deletions(-) create mode 100644 subworkflows/local/report_benchmark_statistics.nf delete mode 100644 subworkflows/local/report_statistics_truth.nf rename subworkflows/local/{report_statistics_test.nf => report_vcf_statistics.nf} (92%) rename subworkflows/local/{somatic_benchmark.nf => small_somatic_benchmark.nf} (93%) delete mode 100644 subworkflows/local/visualizations.nf diff --git a/.gitignore b/.gitignore index fdab1ac..cc9b9e6 100644 --- a/.gitignore +++ b/.gitignore @@ -7,3 +7,5 @@ testing/ testing* *.pyc test_small*/ +test_sv*/ + diff --git a/assets/samplesheet_small.csv b/assets/samplesheet_small.csv index 965d260..3180842 100644 --- a/assets/samplesheet_small.csv +++ b/assets/samplesheet_small.csv @@ -1,2 +1,2 @@ test_vcf,caller,vartype -"https://raw.githubusercontent.com/nf-core/test-datasets/modules//data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",haplotypecaller,small +"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",haplotypecaller,small diff --git a/conf/test.config b/conf/test.config index cb4b3c9..bf9fcb7 100644 --- a/conf/test.config +++ b/conf/test.config @@ -30,9 +30,8 @@ params { analysis = 'germline' method = 'truvari,svanalyzer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. - preprocess = "normalization, deduplication" + preprocess = "normalization, deduplication, prepy" min_sv_size = 50 - standardization = true truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" } diff --git a/conf/test_small.config b/conf/test_small.config index b998525..a64ed2b 100644 --- a/conf/test_small.config +++ b/conf/test_small.config @@ -32,5 +32,6 @@ params { preprocess = "normalization,deduplication,prepy" //min_sv_size = 50 - truth_small = "https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz" + truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" } diff --git a/nextflow.config b/nextflow.config index 66932f2..6d81c72 100644 --- a/nextflow.config +++ b/nextflow.config @@ -19,27 +19,21 @@ params { // Preprocess spesific parameters // normalization includes braking down multiallelic samples -m any // deduplication removes one of the variants in the same position - preprocess = "" - standardization = false - harmonize = false - high_conf = "" + preprocess = "" //normalization,deduplication,prepy,filter_contigs + sv_standardization = "" // harmonize,standardization,dup_to_ins,bnd_to_inv,gridss_annotate // Benchmarking method - method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist + method = 'truvari,svanalyzer,happy,rtgtools' // --not working for now : wittyer, vcfdist // minsize effects both truvari and variantbenchmarkingmark in different ways! svbechmark filters both base and comp calls // although with truvari it is possible to filter only base, which makes more sense to me! // I will use this parameter to filter SVs before benchmarking! min_sv_size = 50 - // Truvari params - dup_to_ins = false // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 - - // Manta params - bnd_to_inv = false // manta reports INV as BND - - // Gridss params - gridss_annotate = false + // TODO: VCF filtering parameters? + // pass_only + // MAPQ + // QUAL // References diff --git a/nextflow_schema.json b/nextflow_schema.json index ce6d39b..4b4bcf0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -29,22 +29,58 @@ "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", "fa_icon": "fas fa-folder-open" }, - "high_conf": { + "high_conf_small": { "type": "string", "format": "file-path", "exists": false, "pattern": "^\\S+\\.(bed)?(\\.gz)?$", - "description": "Path to the high confidence BED files.", - "help_text": "High confidence BED files", + "description": "SMALL: Path to the high confidence BED files.", + "help_text": "SMALL: High confidence BED files", "fa_icon": "fas fa-file-csv" }, - "truth": { + "truth_small": { "type": "string", "format": "file-path", "exists": true, "pattern": "^\\S+\\.(vcf)?(\\.gz)?$", - "description": "Path to the golden set VCF files.", - "help_text": "Truth or golden set VCF file, to be used for comparisons", + "description": "SMALL: Path to the golden set VCF files.", + "help_text": "SMALL: Truth or golden set VCF file, to be used for comparisons", + "fa_icon": "fas fa-file-csv" + }, + "high_conf_sv": { + "type": "string", + "format": "file-path", + "exists": false, + "pattern": "^\\S+\\.(bed)?(\\.gz)?$", + "description": "SV: Path to the high confidence BED files.", + "help_text": "SV: High confidence BED files", + "fa_icon": "fas fa-file-csv" + }, + "truth_sv": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.(vcf)?(\\.gz)?$", + "description": " SV: Path to the golden set VCF files.", + "help_text": "SV: Truth or golden set VCF file, to be used for comparisons", + "fa_icon": "fas fa-file-csv" + }, + "high_conf_cnv": { + "type": "string", + "format": "file-path", + "exists": false, + "pattern": "^\\S+\\.(bed)?(\\.gz)?$", + "description": "CNV: Path to the high confidence BED files.", + "help_text": "CNV: High confidence BED files", + "fa_icon": "fas fa-file-csv" + }, + "truth_cnv": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.(vcf)?(\\.gz)?$", + "description": "CNV: Path to the golden set VCF files.", + "help_text": "CNV: Truth or golden set VCF file, to be used for comparisons", "fa_icon": "fas fa-file-csv" }, "analysis": { @@ -59,14 +95,14 @@ "description": "", "fa_icon": "fas fa-folder-open" }, - "standardization": { - "type": "boolean", + "sv_standardization": { + "type": "string", "format": "directory-path", "description": "", "fa_icon": "fas fa-folder-open" }, - "harmonize": { - "type": "boolean", + "similarity": { + "type": "integer", "format": "directory-path", "description": "", "fa_icon": "fas fa-folder-open" @@ -83,24 +119,6 @@ "description": "", "fa_icon": "fas fa-folder-open" }, - "dup_to_ins": { - "type": "boolean", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "bnd_to_inv": { - "type": "boolean", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, - "gridss_annotate": { - "type": "boolean", - "format": "directory-path", - "description": "", - "fa_icon": "fas fa-folder-open" - }, "email": { "type": "string", "description": "Email address for completion summary.", diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 73563fa..d2846c6 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -62,20 +62,21 @@ workflow PREPARE_VCFS_TEST { ) vcf_ch = TABIX_BGZIPTABIX_1.out.gz_tbi - // - // BCFTOOLS_VIEW - // - // To filter out contigs! - BCFTOOLS_VIEW( - vcf_ch - ) - versions = versions.mix(BCFTOOLS_VIEW.out.versions) - - TABIX_BGZIPTABIX_2( - BCFTOOLS_VIEW.out.vcf - ) - vcf_ch = TABIX_BGZIPTABIX_2.out.gz_tbi + if (params.preprocess.contains("filter_contigs")){ + // + // BCFTOOLS_VIEW + // + // To filter out contigs! + BCFTOOLS_VIEW( + vcf_ch + ) + versions = versions.mix(BCFTOOLS_VIEW.out.versions) + TABIX_BGZIPTABIX_2( + BCFTOOLS_VIEW.out.vcf + ) + vcf_ch = TABIX_BGZIPTABIX_2.out.gz_tbi + } if (params.preprocess.contains("normalization")){ // // BCFTOOLS_NORM @@ -94,11 +95,20 @@ workflow PREPARE_VCFS_TEST { BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) .set{vcf_ch} } - // TODO: this part should onyl run for SV bench + + vcf_ch.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{vcf} + + out_vcf_ch = Channel.empty() + // TODO: this part should only run for SV bench if (params.min_sv_size > 0){ TABIX_BGZIP( - vcf_ch.map{it -> tuple( it[0], it[1])} + vcf.sv.map{it -> tuple( it[0], it[1])} ) versions = versions.mix(TABIX_BGZIP.out.versions) @@ -119,8 +129,13 @@ workflow PREPARE_VCFS_TEST { SURVIVOR_FILTER.out.vcf ) vcf_ch = TABIX_BGZIPTABIX_3.out.gz_tbi - } + out_vcf_ch = out_vcf_ch.mix(vcf_ch) + // TODO: Filter SVs from small variant calling? + out_vcf_ch = out_vcf_ch.mix(vcf.small) + out_vcf_ch = out_vcf_ch.mix(vcf.cnv) + vcf_ch = out_vcf_ch + } if (params.preprocess.contains("deduplication")){ // // BCFTOOLS_NORM @@ -139,10 +154,20 @@ workflow PREPARE_VCFS_TEST { BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) .set{vcf_ch} } + + vcf_ch.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{vcf} + + out_vcf_ch = Channel.empty() + // only for small variant benchmarking if (params.preprocess.contains("prepy")){ HAPPY_PREPY( - vcf_ch.map{it -> tuple( it[0], it[1],[])}, + vcf.small.map{it -> tuple( it[0], it[1],[])}, fasta, fai ) @@ -154,6 +179,11 @@ workflow PREPARE_VCFS_TEST { HAPPY_PREPY.out.preprocessed_vcf.join(TABIX_TABIX_3.out.tbi, by:0) .set{vcf_ch} + + out_vcf_ch = out_vcf_ch.mix(vcf_ch) + out_vcf_ch = out_vcf_ch.mix(vcf.sv) + out_vcf_ch = out_vcf_ch.mix(vcf.cnv) + vcf_ch = out_vcf_ch } emit: diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 223e468..b87f6ff 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -94,7 +94,6 @@ workflow PREPARE_VCFS_TRUTH { .set{vcf_ch} } - emit: vcf_ch versions diff --git a/subworkflows/local/report_benchmark_statistics.nf b/subworkflows/local/report_benchmark_statistics.nf new file mode 100644 index 0000000..4acf1e5 --- /dev/null +++ b/subworkflows/local/report_benchmark_statistics.nf @@ -0,0 +1,28 @@ +// +// REPORT_BENCHMARK_STATISTICS: BENCHMARK REPORTS +// + +params.options = [:] + +include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix.nf' addParams( options: params.options ) +include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) + +workflow REPORT_BENCHMARK_STATISTICS { + take: + truth_ch // channel: [val(meta), vcf] + ref // reference channel [ref.fa, ref.fa.fai] + main_chroms // channel: path(chrom sizes) + + main: + + versions=Channel.empty() + +// Check tool spesific conversions + + // https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R#L9 + // GRIDSS simple event annotation + + emit: + vcf_ch + versions +} diff --git a/subworkflows/local/report_statistics_truth.nf b/subworkflows/local/report_statistics_truth.nf deleted file mode 100644 index 2cdeab1..0000000 --- a/subworkflows/local/report_statistics_truth.nf +++ /dev/null @@ -1,51 +0,0 @@ -// -// REPORT_STATISTICS_TRUTH: SUBWORKFLOW TO REPORT VCF STATS -// - -params.options = [:] - -include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' addParams( options: params.options ) -include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' addParams( options: params.options ) - -workflow REPORT_STATISTICS_TRUTH { - take: - input_ch // channel: [val(meta), vcf, index] - - main: - - versions=Channel.empty() - - // - // SURVIVOR_STATS - // - SURVIVOR_STATS( - input_ch.map{it -> tuple( it[0], it[1])}, - -1, - -1, - -1 - ) - survivor_stats = SURVIVOR_STATS.out.stats - versions = versions.mix(SURVIVOR_STATS.out.versions) - - // - // BCFTOOLS_STATS - // - BCFTOOLS_STATS( - input_ch, - [[],[]], - [[],[]], - [[],[]], - [[],[]], - [[],[]] - ) - bcftools_stats = BCFTOOLS_STATS.out.stats - versions = versions.mix(BCFTOOLS_STATS.out.versions) - - - // Add here a tool, to visualize SV statistics in a histogram. - - emit: - bcftools_stats - survivor_stats - versions -} diff --git a/subworkflows/local/report_statistics_test.nf b/subworkflows/local/report_vcf_statistics.nf similarity index 92% rename from subworkflows/local/report_statistics_test.nf rename to subworkflows/local/report_vcf_statistics.nf index 74bd35a..4a3a195 100644 --- a/subworkflows/local/report_statistics_test.nf +++ b/subworkflows/local/report_vcf_statistics.nf @@ -1,5 +1,5 @@ // -// REPORT_STATISTICS_TEST: SUBWORKFLOW TO REPORT VCF STATS +// REPORT_VCF_STATISTICS: SUBWORKFLOW TO REPORT VCF STATS // params.options = [:] @@ -7,7 +7,7 @@ params.options = [:] include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' addParams( options: params.options ) include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' addParams( options: params.options ) -workflow REPORT_STATISTICS_TEST { +workflow REPORT_VCF_STATISTICS { take: input_ch // channel: [val(meta), vcf, index] diff --git a/subworkflows/local/somatic_benchmark.nf b/subworkflows/local/small_somatic_benchmark.nf similarity index 93% rename from subworkflows/local/somatic_benchmark.nf rename to subworkflows/local/small_somatic_benchmark.nf index b951066..3554fec 100644 --- a/subworkflows/local/somatic_benchmark.nf +++ b/subworkflows/local/small_somatic_benchmark.nf @@ -1,5 +1,5 @@ // -// SOMATIC: SUBWORKFLOW FOR SOMATIC VARIANTS +// SOMATIC: SUBWORKFLOW FOR SMALL SOMATIC VARIANTS // params.options = [:] @@ -7,7 +7,7 @@ params.options = [:] include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' addParams( options: params.options ) include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' addParams( options: params.options ) -workflow SOMATIC_BENCHMARK { +workflow SMALL_SOMATIC_BENCHMARK { take: input_ch // channel: [val(meta), test_vcf,test_index, truth_vcf, truth_index, bed] fasta // reference channel [val(meta), ref.fa] diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 3f2988b..1f0e61d 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -34,7 +34,7 @@ workflow SV_VCF_CONVERSIONS { // MODULE: SVYNC // // - if(params.standardization){ + if(params.sv_standardization.contains("standardization")){ out_vcf_ch = Channel.empty() vcf_ch.branch{ @@ -59,7 +59,7 @@ workflow SV_VCF_CONVERSIONS { } // Check tool spesific conversions - if(params.bnd_to_inv){ + if(params.sv_standardization.contains("bnd_to_inv")){ out_vcf_ch = Channel.empty() vcf_ch.branch{ @@ -87,7 +87,7 @@ workflow SV_VCF_CONVERSIONS { } - if (params.gridss_annotate){ + if (params.sv_standardization.contains("gridss_annotate")){ out_vcf_ch = Channel.empty() vcf_ch.branch{ diff --git a/subworkflows/local/visualizations.nf b/subworkflows/local/visualizations.nf deleted file mode 100644 index d72d3d6..0000000 --- a/subworkflows/local/visualizations.nf +++ /dev/null @@ -1,35 +0,0 @@ -// -// PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS -// - -params.options = [:] - -include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix.nf' addParams( options: params.options ) -include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_3 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { BGZIP_TABIX as BGZIP_TABIX_1 } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) -include { BGZIP_TABIX as BGZIP_TABIX_2 } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) - -workflow PREPARE_VCFS_TRUTH { - take: - truth_ch // channel: [val(meta), vcf] - ref // reference channel [ref.fa, ref.fa.fai] - main_chroms // channel: path(chrom sizes) - - main: - - versions=Channel.empty() - -// Check tool spesific conversions - - // https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R#L9 - // GRIDSS simple event annotation - - emit: - vcf_ch - versions -} diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index fde91b4..a27f571 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -23,14 +23,12 @@ include { paramsSummaryMap } from 'plugin/nf-validation' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' -include { SOMATIC_BENCHMARK } from '../subworkflows/local/somatic_benchmark' include { SV_GERMLINE_BENCHMARK } from '../subworkflows/local/sv_germline_benchmark' include { SMALL_GERMLINE_BENCHMARK } from '../subworkflows/local/small_germline_benchmark' include { PREPARE_VCFS_TRUTH } from '../subworkflows/local/prepare_vcfs_truth' include { PREPARE_VCFS_TEST } from '../subworkflows/local/prepare_vcfs_test' include { SV_VCF_CONVERSIONS } from '../subworkflows/local/sv_vcf_conversion' -include { REPORT_STATISTICS_TRUTH } from '../subworkflows/local/report_statistics_truth.nf' -include { REPORT_STATISTICS_TEST } from '../subworkflows/local/report_statistics_test.nf' +include { REPORT_VCF_STATISTICS } from '../subworkflows/local/report_vcf_statistics' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -135,17 +133,11 @@ workflow VARIANTBENCHMARKING { // // SUBWORKFLOW: GET STATISTICS OF FILES // - //REPORT_STATISTICS_TRUTH( - // - REPORT_STATISTICS_TEST( - PREPARE_VCFS_TEST.out.vcf_ch + REPORT_VCF_STATISTICS( + PREPARE_VCFS_TEST.out.vcf_ch.mix(PREPARE_VCFS_TRUTH.out.vcf_ch) ) - ch_versions = ch_versions.mix(REPORT_STATISTICS_TEST.out.versions) + ch_versions = ch_versions.mix(REPORT_VCF_STATISTICS.out.versions) - REPORT_STATISTICS_TRUTH( - PREPARE_VCFS_TRUTH.out.vcf_ch - ) - ch_versions = ch_versions.mix(REPORT_STATISTICS_TRUTH.out.versions) // prepare benchmark set if (params.high_conf_small || params.high_conf_sv || params.high_conf_cnv ){ @@ -159,7 +151,6 @@ workflow VARIANTBENCHMARKING { } // BENCHMARKS - bench_ch.branch{ sv: it[0].vartype == "sv" small: it[0].vartype == "small" @@ -189,7 +180,7 @@ workflow VARIANTBENCHMARKING { fai ) ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) - // TODO: SOMATIC EBNCHMARKING + // TODO: SOMATIC BENCHMARKING if (params.analysis.contains("somatic")){ // SOMATIC VARIANT BENCHMARKING From 0a85a0bce7b84cd970d00f7fc989608c85eb97c6 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Mon, 18 Mar 2024 13:53:21 +0100 Subject: [PATCH 042/292] rewrite svync handling => now more dynamic --- subworkflows/local/sv_vcf_conversion.nf | 30 ++++++++++++++++--------- workflows/variantbenchmarking.nf | 5 +---- 2 files changed, 20 insertions(+), 15 deletions(-) diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 09ab7c9..ba4875b 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -1,3 +1,5 @@ +import groovy.io.FileType + // // SV_VCF_CONVERSIONS: SUBWORKFLOW TO apply tool spesific conversions // @@ -13,7 +15,6 @@ workflow SV_VCF_CONVERSIONS { take: input_ch // channel: [val(meta), vcf] ref // reference channel [ref.fa, ref.fa.fai] - svync_yaml // yaml configs main: versions = Channel.empty() @@ -35,19 +36,26 @@ workflow SV_VCF_CONVERSIONS { // if(params.standardization){ out_vcf_ch = Channel.empty() - - vcf_ch.branch{ - tool: it[0].id == "delly" || it[0].id == "gridss" || it[0].id == "manta" || it[0].id == "smoove" - other: true} + supported_callers = [] + new File("${projectDir}/assets/svync").eachFileRecurse (FileType.FILES) { supported_callers << it.baseName.replace(".yaml", "") } + + vcf_ch + .branch{ + def supported = supported_callers.contains(it[0].id) + if(!supported) { + log.warn("Standardization for SV caller '${it[0].id}' is not supported. Skipping standardization...") + } + tool: supported + other: !supported + } .set{input} - svync_yaml.flatten() - .map { it -> tuple([id: it.getSimpleName(), vartype: "sv"], it) } - .set{config} - input.tool - .combine(config, by:0) - .set{svync_ch} + .map { meta, vcf, tbi -> + config = file("${projectDir}/assets/svync/${meta.id}.yaml", checkIfExists:true) + [ meta, vcf, tbi, config ] + } + .set {svync_ch} SVYNC( svync_ch diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index c3c9be0..1d5391d 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -60,8 +60,6 @@ workflow VARIANTBENCHMARKING { high_conf = params.high_conf ? Channel.fromPath(params.high_conf, checkIfExists: true).collect() : Channel.empty() - svync_yaml = params.standardization ? Channel.fromPath("assets/svync/*.yaml").collect() - : Channel.empty() // TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID @@ -81,8 +79,7 @@ workflow VARIANTBENCHMARKING { // Standardize SV VCFs, tool spesific modifications SV_VCF_CONVERSIONS( input.sv, - ref, - svync_yaml + ref ) ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) out_vcf_ch = out_vcf_ch.mix(SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])}) From 1a17ac9a94b19025ed0eb582626507b5f8dde8c4 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 18 Mar 2024 13:49:36 +0000 Subject: [PATCH 043/292] fix lint --- nextflow.config | 9 +++++++-- 1 file changed, 7 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 6d81c72..06667a8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,14 +13,19 @@ params { // Input options input = null outdir = "results" - truth = null - high_conf = null + truth_small = null + high_conf_small = null + truth_sv = null + high_conf_sv = null + truth_cnv = null + high_conf_cnv = null // Preprocess spesific parameters // normalization includes braking down multiallelic samples -m any // deduplication removes one of the variants in the same position preprocess = "" //normalization,deduplication,prepy,filter_contigs sv_standardization = "" // harmonize,standardization,dup_to_ins,bnd_to_inv,gridss_annotate + similarity = null // Benchmarking method method = 'truvari,svanalyzer,happy,rtgtools' // --not working for now : wittyer, vcfdist From 16b0087e7e676d299f57ae34a9c16ee16181a4c4 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 19 Mar 2024 09:15:52 +0000 Subject: [PATCH 044/292] dragen file --- assets/samplesheet_HG002_hg38.csv | 1 + conf/test_hg38.config | 14 ++------------ 2 files changed, 3 insertions(+), 12 deletions(-) diff --git a/assets/samplesheet_HG002_hg38.csv b/assets/samplesheet_HG002_hg38.csv index c080639..3b958e2 100644 --- a/assets/samplesheet_HG002_hg38.csv +++ b/assets/samplesheet_HG002_hg38.csv @@ -2,3 +2,4 @@ test_vcf,caller,vartype https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.chr21.vcf.gz,lumpy,sv https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,sv https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,sv +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,sv diff --git a/conf/test_hg38.config b/conf/test_hg38.config index aaeb3fd..ed8bdf4 100644 --- a/conf/test_hg38.config +++ b/conf/test_hg38.config @@ -36,19 +36,9 @@ params { preprocess = "normalization, deduplication" min_sv_size = 50 - // Truvari params - //dup_to_ins = true // truvari cannot benchmark DUP type, convert DUP type to INS. Has to be used with similarity = 0 - - // Manta params - //bnd_to_inv = true // manta reports INV as BND - - // Gridss params - //gridss_annotate = true - - //standardization = true sample = "HG002" // available samples: SEQC2, HG002 - truth_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.vcf.gz" - high_conf_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg38/HG002_CMRG_V1_Truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.bed" + truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" } From 22c362155d0fb031b3ee4fc66ec5b391da58bab9 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Tue, 19 Mar 2024 11:51:04 +0100 Subject: [PATCH 045/292] fix wrong merge --- subworkflows/local/sv_vcf_conversion.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 82d781e..c54c235 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -16,7 +16,6 @@ workflow SV_VCF_CONVERSIONS { input_ch // channel: [val(meta), vcf] fasta // reference channel [val(meta), ref.fa] fai // reference channel [val(meta), ref.fa.fai] - svync_yaml // yaml configs main: versions = Channel.empty() From 0f951b6b95b13c5292c3b3524ea958001a608ef7 Mon Sep 17 00:00:00 2001 From: Nicolas Vannieuwkerke Date: Tue, 19 Mar 2024 13:44:35 +0100 Subject: [PATCH 046/292] add support for custom svync configs --- nextflow.config | 1 + nextflow_schema.json | 9 +++++++++ subworkflows/local/sv_vcf_conversion.nf | 21 ++++++++++++++++----- workflows/variantbenchmarking.nf | 6 +++++- 4 files changed, 31 insertions(+), 6 deletions(-) diff --git a/nextflow.config b/nextflow.config index 06667a8..a27cd87 100644 --- a/nextflow.config +++ b/nextflow.config @@ -19,6 +19,7 @@ params { high_conf_sv = null truth_cnv = null high_conf_cnv = null + svync_yaml = null // Preprocess spesific parameters // normalization includes braking down multiallelic samples -m any diff --git a/nextflow_schema.json b/nextflow_schema.json index 4b4bcf0..86be229 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -83,6 +83,15 @@ "help_text": "CNV: Truth or golden set VCF file, to be used for comparisons", "fa_icon": "fas fa-file-csv" }, + "svync_yaml": { + "type": "string", + "format": "file-path-pattern", + "exists": true, + "pattern": "^\\S+\\.yaml$", + "description": "Custom configuration files for Svync standardization", + "help_text": "Please make sure the caller is the basename of the file (e.g. for lumpy, the config should called 'lumpy.yaml'", + "errorMessage": "A path/glob pattern to existing config files should be given. These files should have the 'yaml' extension" + }, "analysis": { "type": "string", "format": "directory-path", diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index c54c235..4f6a235 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -16,6 +16,7 @@ workflow SV_VCF_CONVERSIONS { input_ch // channel: [val(meta), vcf] fasta // reference channel [val(meta), ref.fa] fai // reference channel [val(meta), ref.fa.fai] + svync_yaml // reference channel [yamls] main: versions = Channel.empty() @@ -40,20 +41,30 @@ workflow SV_VCF_CONVERSIONS { supported_callers = [] new File("${projectDir}/assets/svync").eachFileRecurse (FileType.FILES) { supported_callers << it.baseName.replace(".yaml", "") } + svync_yaml + .map { yamls -> + [yamls.collect { it.baseName }, yamls.collectEntries { [it.baseName, it] }] + } + .set { custom_svync_configs } + vcf_ch - .branch{ - def supported = supported_callers.contains(it[0].id) + .combine(custom_svync_configs) + .branch{ meta, vcf, tbi, custom_callers, custom_configs -> + def caller = meta.id + def supported = custom_callers.contains(caller) || supported_callers.contains(caller) if(!supported) { - log.warn("Standardization for SV caller '${it[0].id}' is not supported. Skipping standardization...") + log.warn("Standardization for SV caller '${caller}' is not supported. Skipping standardization...") } tool: supported + return [ meta, vcf, tbi, custom_configs[caller] ?: [] ] other: !supported + return [ meta, vcf, tbi ] } .set{input} input.tool - .map { meta, vcf, tbi -> - config = file("${projectDir}/assets/svync/${meta.id}.yaml", checkIfExists:true) + .map { meta, vcf, tbi, custom_config -> + config = custom_config ?: file("${projectDir}/assets/svync/${meta.id}.yaml", checkIfExists:true) [ meta, vcf, tbi, config ] } .set {svync_ch} diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 394d85c..606ff01 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -81,6 +81,9 @@ workflow VARIANTBENCHMARKING { : Channel.empty() high_conf_ch = high_conf_ch.mix(high_conf_cnv) + svync_yaml = params.svync_yaml ? Channel.fromPath(params.svync_yaml, checkIfExists: true).collect() + : Channel.empty() + // TODO: GET FILES FROM IGENOMES ACCORDING TO META.ID @@ -101,7 +104,8 @@ workflow VARIANTBENCHMARKING { SV_VCF_CONVERSIONS( input.sv, fasta, - fai + fai, + svync_yaml ) ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) out_vcf_ch = out_vcf_ch.mix(SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])}) From 8fbdca4c42940473e829ff5b3d7dfbb226fe68e8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 20 Mar 2024 08:42:17 +0000 Subject: [PATCH 047/292] bcftools norm -c s and bcftools sort added --- conf/modules.config | 25 +++++--- conf/test_hg38.config | 5 +- modules.json | 5 ++ modules/local/wittyer.nf | 11 ++-- modules/nf-core/bcftools/sort/environment.yml | 7 +++ modules/nf-core/bcftools/sort/main.nf | 61 +++++++++++++++++++ modules/nf-core/bcftools/sort/meta.yml | 42 +++++++++++++ .../nf-core/bcftools/sort/tests/main.nf.test | 32 ++++++++++ .../bcftools/sort/tests/main.nf.test.snap | 35 +++++++++++ modules/nf-core/bcftools/sort/tests/tags.yml | 2 + subworkflows/local/prepare_vcfs_test.nf | 14 ++++- subworkflows/local/sv_germline_benchmark.nf | 4 +- workflows/variantbenchmarking.nf | 7 ++- 13 files changed, 223 insertions(+), 27 deletions(-) create mode 100644 modules/nf-core/bcftools/sort/environment.yml create mode 100644 modules/nf-core/bcftools/sort/main.nf create mode 100644 modules/nf-core/bcftools/sort/meta.yml create mode 100644 modules/nf-core/bcftools/sort/tests/main.nf.test create mode 100644 modules/nf-core/bcftools/sort/tests/main.nf.test.snap create mode 100644 modules/nf-core/bcftools/sort/tests/tags.yml diff --git a/conf/modules.config b/conf/modules.config index 215fc02..f9b0728 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -28,21 +28,29 @@ process { } withName: "BCFTOOLS_NORM_1" { ext.prefix = {"${params.sample}"} - ext.args = {"--output-type z -N -m-any -c w" } + ext.args = {"--output-type z -N -m-any -c s" } ext.prefix = {"${meta.id}_norm"} publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, - pattern: "*{.vcf.gz,vcf.gz.tbi}", - mode: params.publish_dir_mode + path: { "${params.outdir}/test" }, + enabled: false ] } withName: "BCFTOOLS_NORM_2" { ext.prefix = {"${params.sample}"} - ext.args = {"--output-type z --rm-du exact -c w" } + ext.args = {"--output-type z --rm-du exact -c s" } ext.prefix = {"${meta.id}_dedup"} + publishDir = [ + path: { "${params.outdir}/test" }, + enabled: false + ] + } + withName: "BCFTOOLS_SORT" { + ext.prefix = {"${params.sample}"} + ext.args = {"--output-type z" } + ext.prefix = {"${meta.id}_sort"} publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, - pattern: "*{.vcf.gz,vcf.gz.tbi}", + pattern: "*{.vcf.gz}", mode: params.publish_dir_mode ] } @@ -160,10 +168,9 @@ process { } withName: WITTYER { ext.prefix = {"${params.sample}"} - ext.args = {"--evaluationMode=CrossTypeAndSimpleCounting --percentDistance=0.3 --bpDistance=1000"} publishDir = [ path: {"${params.outdir}/${meta.id}/wittyer_bench"}, - pattern: "*{json,.vcf.gz.tbi,vcf.gz}", + pattern: "*{json,vcf.gz.tbi,vcf.gz}", mode: params.publish_dir_mode ] } @@ -213,7 +220,7 @@ process { // Don't publish results for these processes // process { - withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX|TABIX_BGZIP|RTGTOOLS_FORMAT' { + withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX|RTGTOOLS_FORMAT' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false diff --git a/conf/test_hg38.config b/conf/test_hg38.config index ed8bdf4..0535bfc 100644 --- a/conf/test_hg38.config +++ b/conf/test_hg38.config @@ -30,8 +30,7 @@ params { // Processes analysis = 'germline' //somatic - method = 'truvari,svanalyzer,wittyer' // --not working for now : vcfdist - //harmonize = true // ATTENTION : takes long time because of multi-alignment process. + method = 'truvari,svanalyzer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. preprocess = "normalization, deduplication" min_sv_size = 50 @@ -39,6 +38,6 @@ params { sample = "HG002" // available samples: SEQC2, HG002 truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + //high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" } diff --git a/modules.json b/modules.json index 78603d7..b19094d 100644 --- a/modules.json +++ b/modules.json @@ -15,6 +15,11 @@ "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", "installed_by": ["modules"] }, + "bcftools/sort": { + "branch": "master", + "git_sha": "487d92367b4d7bb9f1ca694bf72736be90720b15", + "installed_by": ["modules"] + }, "bcftools/stats": { "branch": "master", "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", diff --git a/modules/local/wittyer.nf b/modules/local/wittyer.nf index 57973e7..43c0a83 100644 --- a/modules/local/wittyer.nf +++ b/modules/local/wittyer.nf @@ -9,7 +9,6 @@ process WITTYER { input: tuple val(meta),path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) - path(config) output: tuple val(meta), path("*ConfigFileUsed.json") , emit: config @@ -24,17 +23,15 @@ process WITTYER { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def regions = bed ? "--includeBed=$bed" : "" - def config = config ? "--configFile=$config" : "" + def regions = bed ? "-b $bed" : "" """ mkdir bench dotnet /opt/Wittyer/Wittyer.dll \\ - --truthVcf=${truth_vcf} \\ - --inputVcf=${vcf} \\ - --outputDirectory=bench \\ + -i ${vcf} \\ + -t ${truth_vcf} \\ + -o bench \\ ${regions} \\ - ${config} \\ ${args} mv bench/Wittyer.ConfigFileUsed.json ${prefix}.ConfigFileUsed.json diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml new file mode 100644 index 0000000..89cf911 --- /dev/null +++ b/modules/nf-core/bcftools/sort/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_sort +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf new file mode 100644 index 0000000..c982944 --- /dev/null +++ b/modules/nf-core/bcftools/sort/main.nf @@ -0,0 +1,61 @@ +process BCFTOOLS_SORT { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta), path(vcf) + + output: + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + """ + bcftools \\ + sort \\ + --output ${prefix}.${extension} \\ + --temp-dir . \\ + $args \\ + $vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '--output-type z' + def prefix = task.ext.prefix ?: "${meta.id}" + + def extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + """ + touch ${prefix}.${extension} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml new file mode 100644 index 0000000..84747c6 --- /dev/null +++ b/modules/nf-core/bcftools/sort/meta.yml @@ -0,0 +1,42 @@ +name: bcftools_sort +description: Sorts VCF files +keywords: + - sorting + - VCF + - variant calling +tools: + - sort: + description: Sort VCF files by coordinates. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + tool_dev_url: https://github.com/samtools/bcftools + doi: "10.1093/bioinformatics/btp352" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: The VCF/BCF file to be sorted + pattern: "*.{vcf.gz,vcf,bcf}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - vcf: + type: file + description: Sorted VCF file + pattern: "*.{vcf.gz}" +authors: + - "@Gwennid" +maintainers: + - "@Gwennid" diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test b/modules/nf-core/bcftools/sort/tests/main.nf.test new file mode 100644 index 0000000..fec59cf --- /dev/null +++ b/modules/nf-core/bcftools/sort/tests/main.nf.test @@ -0,0 +1,32 @@ +nextflow_process { + + name "Test Process BCFTOOLS_SORT" + script "../main.nf" + process "BCFTOOLS_SORT" + + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/sort" + + test("SarsCov2 VCF") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } +} diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test.snap b/modules/nf-core/bcftools/sort/tests/main.nf.test.snap new file mode 100644 index 0000000..7f59955 --- /dev/null +++ b/modules/nf-core/bcftools/sort/tests/main.nf.test.snap @@ -0,0 +1,35 @@ +{ + "SarsCov2 VCF": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + "versions.yml:md5,622bd32d4ff0fac3360cd534ae0f0168" + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,622bd32d4ff0fac3360cd534ae0f0168" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.2" + }, + "timestamp": "2024-03-18T12:50:10.340362246" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/sort/tests/tags.yml b/modules/nf-core/bcftools/sort/tests/tags.yml new file mode 100644 index 0000000..6e9520d --- /dev/null +++ b/modules/nf-core/bcftools/sort/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/sort: + - "modules/nf-core/bcftools/sort/**" diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index d2846c6..c1f369e 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -6,6 +6,7 @@ params.options = [:] include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' addParams( options: params.options ) include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' addParams( options: params.options ) @@ -77,6 +78,7 @@ workflow PREPARE_VCFS_TEST { ) vcf_ch = TABIX_BGZIPTABIX_2.out.gz_tbi } + if (params.preprocess.contains("normalization")){ // // BCFTOOLS_NORM @@ -104,7 +106,7 @@ workflow PREPARE_VCFS_TEST { .set{vcf} out_vcf_ch = Channel.empty() - // TODO: this part should only run for SV bench + if (params.min_sv_size > 0){ TABIX_BGZIP( @@ -147,11 +149,16 @@ workflow PREPARE_VCFS_TEST { ) versions = versions.mix(BCFTOOLS_NORM_2.out.versions) - TABIX_TABIX_2( + // sort vcf + BCFTOOLS_SORT( BCFTOOLS_NORM_2.out.vcf ) - BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) + TABIX_TABIX_2( + BCFTOOLS_SORT.out.vcf + ) + + BCFTOOLS_SORT.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) .set{vcf_ch} } @@ -186,6 +193,7 @@ workflow PREPARE_VCFS_TEST { vcf_ch = out_vcf_ch } + emit: vcf_ch versions diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index 58783e0..1a068fd 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -62,14 +62,14 @@ workflow SV_GERMLINE_BENCHMARK { } if (params.method.contains('wittyer')){ + // // MODULE: WITTYER // // BIG Advantage: reports by variant type // Able to report CNV WITTYER( - input_ch, - [] + input_ch ) versions = versions.mix(WITTYER.out.versions) } diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index a27f571..c861bda 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -141,12 +141,13 @@ workflow VARIANTBENCHMARKING { // prepare benchmark set if (params.high_conf_small || params.high_conf_sv || params.high_conf_cnv ){ - PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) + PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch) .combine(high_conf_ch) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} .set{bench_ch} }else{ - PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch.map { it -> tuple(it[1], it[2]) }) - .map{it -> tuple(it[0], it[1], it[2],it[3],it[4],[])} + PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} .set{bench_ch} } From 9516986a6bdb9261ec5d9cc68cef53f5e33b6c7c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 20 Mar 2024 12:16:25 +0000 Subject: [PATCH 048/292] subworkflows added --- .gitignore | 1 + conf/modules.config | 4 +- subworkflows/local/prepare_vcfs_test.nf | 62 ++++--------------- subworkflows/local/prepare_vcfs_truth.nf | 38 +++++------- subworkflows/local/sv_variant_filtering.nf | 46 ++++++++++++++ .../local/vcf_variant_deduplication.nf | 47 ++++++++++++++ 6 files changed, 123 insertions(+), 75 deletions(-) create mode 100644 subworkflows/local/sv_variant_filtering.nf create mode 100644 subworkflows/local/vcf_variant_deduplication.nf diff --git a/.gitignore b/.gitignore index cc9b9e6..6e0bd82 100644 --- a/.gitignore +++ b/.gitignore @@ -8,4 +8,5 @@ testing* *.pyc test_small*/ test_sv*/ +test/ diff --git a/conf/modules.config b/conf/modules.config index f9b0728..1acc629 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -26,7 +26,7 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } - withName: "BCFTOOLS_NORM_1" { + withName: "BCFTOOLS_NORM" { ext.prefix = {"${params.sample}"} ext.args = {"--output-type z -N -m-any -c s" } ext.prefix = {"${meta.id}_norm"} @@ -35,7 +35,7 @@ process { enabled: false ] } - withName: "BCFTOOLS_NORM_2" { + withName: "BCFTOOLS_DEDUP" { ext.prefix = {"${params.sample}"} ext.args = {"--output-type z --rm-du exact -c s" } ext.prefix = {"${meta.id}_dedup"} diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index c1f369e..a0260bf 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -7,17 +7,14 @@ params.options = [:] include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' addParams( options: params.options ) -include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) -include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' addParams( options: params.options ) +include { SV_VARIANT_FILTERING } from '../local/sv_variant_filtering' addParams( options: params.options ) include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) include { TABIX_TABIX as TABIX_TABIX_3 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_1 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_2 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_3 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TEST } from '../../modules/nf-core/bcftools/reheader' addParams( options: params.options ) @@ -84,17 +81,17 @@ workflow PREPARE_VCFS_TEST { // BCFTOOLS_NORM // // Breaks down -any- multi-allelic variants - BCFTOOLS_NORM_1( + BCFTOOLS_NORM( vcf_ch, fasta ) - versions = versions.mix(BCFTOOLS_NORM_1.out.versions) + versions = versions.mix(BCFTOOLS_NORM.out.versions) TABIX_TABIX_1( - BCFTOOLS_NORM_1.out.vcf + BCFTOOLS_NORM.out.vcf ) - BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) + BCFTOOLS_NORM.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) .set{vcf_ch} } @@ -109,59 +106,26 @@ workflow PREPARE_VCFS_TEST { if (params.min_sv_size > 0){ - TABIX_BGZIP( - vcf.sv.map{it -> tuple( it[0], it[1])} + SV_VARIANT_FILTERING( + vcf.sv ) - versions = versions.mix(TABIX_BGZIP.out.versions) - - // - // MODULE: SURVIVOR_FILTER - // - // filters out smaller SVs than min_sv_size - SURVIVOR_FILTER( - TABIX_BGZIP.out.output.map{it -> tuple( it[0], it[1],[])}, - params.min_sv_size, - -1, - -1, - -1 - ) - versions = versions.mix(SURVIVOR_FILTER.out.versions) - - TABIX_BGZIPTABIX_3( - SURVIVOR_FILTER.out.vcf - ) - vcf_ch = TABIX_BGZIPTABIX_3.out.gz_tbi - out_vcf_ch = out_vcf_ch.mix(vcf_ch) - - // TODO: Filter SVs from small variant calling? + out_vcf_ch = out_vcf_ch.mix(SV_VARIANT_FILTERING.out.vcf_ch) out_vcf_ch = out_vcf_ch.mix(vcf.small) out_vcf_ch = out_vcf_ch.mix(vcf.cnv) vcf_ch = out_vcf_ch } if (params.preprocess.contains("deduplication")){ // - // BCFTOOLS_NORM + // VCF_VARIANT_DEDUPLICATION // // Deduplicates variants at the same position test - BCFTOOLS_NORM_2( + VCF_VARIANT_DEDUPLICATION( vcf_ch, fasta ) - versions = versions.mix(BCFTOOLS_NORM_2.out.versions) - - // sort vcf - BCFTOOLS_SORT( - BCFTOOLS_NORM_2.out.vcf - ) + vcf_ch = VCF_VARIANT_DEDUPLICATION.out.ch_vcf - TABIX_TABIX_2( - BCFTOOLS_SORT.out.vcf - ) - - BCFTOOLS_SORT.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) - .set{vcf_ch} } - vcf_ch.branch{ sv: it[0].vartype == "sv" small: it[0].vartype == "small" diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index b87f6ff..7d02b5b 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -7,12 +7,10 @@ params.options = [:] include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix.nf' addParams( options: params.options ) include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_1 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { BCFTOOLS_NORM as BCFTOOLS_NORM_2 } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_2 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { TABIX_TABIX as TABIX_TABIX_3 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TRUTH } from '../../modules/nf-core/bcftools/reheader' addParams( options: params.options ) +include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) +include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' addParams( options: params.options ) +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TRUTH } from '../../modules/nf-core/bcftools/reheader' addParams( options: params.options ) workflow PREPARE_VCFS_TRUTH { take: @@ -60,38 +58,30 @@ workflow PREPARE_VCFS_TRUTH { // // Normalize test // multi-allelic variants will be splitted. - BCFTOOLS_NORM_1( + BCFTOOLS_NORM( vcf_ch, fasta ) - versions = versions.mix(BCFTOOLS_NORM_1.out.versions) + versions = versions.mix(BCFTOOLS_NORM.out.versions) - TABIX_TABIX_1( - BCFTOOLS_NORM_1.out.vcf + TABIX_TABIX( + BCFTOOLS_NORM.out.vcf ) - versions = versions.mix(TABIX_TABIX_1.out.versions) + versions = versions.mix(TABIX_TABIX.out.versions) - BCFTOOLS_NORM_1.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) + BCFTOOLS_NORM.out.vcf.join(TABIX_TABIX.out.tbi, by:0) .set{vcf_ch} } if (params.preprocess.contains("deduplication")){ // - // MODULE: BCFTOOLS_NORM + // VCF_VARIANT_DEDUPLICATION // - // Deduplicate variants at the same position - BCFTOOLS_NORM_2( + // Deduplicates variants at the same position test + VCF_VARIANT_DEDUPLICATION( vcf_ch, fasta ) - versions = versions.mix(BCFTOOLS_NORM_2.out.versions) - - TABIX_TABIX_2( - BCFTOOLS_NORM_2.out.vcf - ) - versions = versions.mix(TABIX_TABIX_2.out.versions) - - BCFTOOLS_NORM_2.out.vcf.join(TABIX_TABIX_2.out.tbi, by:0) - .set{vcf_ch} + vcf_ch = VCF_VARIANT_DEDUPLICATION.out.ch_vcf } emit: diff --git a/subworkflows/local/sv_variant_filtering.nf b/subworkflows/local/sv_variant_filtering.nf new file mode 100644 index 0000000..96e30a8 --- /dev/null +++ b/subworkflows/local/sv_variant_filtering.nf @@ -0,0 +1,46 @@ +// +// SV_VARIANT_FILTERING: filter sv variants using survivor +// + +params.options = [:] + +include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) +include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) + + +workflow SV_VARIANT_FILTERING { + take: + vcf_ch // channel: [val(meta),vcf] + + main: + + versions=Channel.empty() + + TABIX_BGZIP( + vcf_ch.map{it -> tuple( it[0], it[1])} + ) + versions = versions.mix(TABIX_BGZIP.out.versions) + + // + // MODULE: SURVIVOR_FILTER + // + // filters out smaller SVs than min_sv_size + SURVIVOR_FILTER( + TABIX_BGZIP.out.output.map{it -> tuple( it[0], it[1],[])}, + params.min_sv_size, + -1, + -1, + -1 + ) + versions = versions.mix(SURVIVOR_FILTER.out.versions) + + TABIX_BGZIPTABIX( + SURVIVOR_FILTER.out.vcf + ) + vcf_ch = TABIX_BGZIPTABIX.out.gz_tbi + + emit: + vcf_ch + versions +} diff --git a/subworkflows/local/vcf_variant_deduplication.nf b/subworkflows/local/vcf_variant_deduplication.nf new file mode 100644 index 0000000..b7a93b2 --- /dev/null +++ b/subworkflows/local/vcf_variant_deduplication.nf @@ -0,0 +1,47 @@ +// +// VCF_VARIANT_DEDUPLICATION: deduplicate, sort and index vcf files +// + +params.options = [:] + +include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' addParams( options: params.options ) +include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) +include { BCFTOOLS_NORM as BCFTOOLS_DEDUP } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) + +workflow VCF_VARIANT_DEDUPLICATION { + take: + vcf_ch // channel: [val(meta),vcf] + fasta // reference channel [val(meta), ref.fa] + + main: + + versions=Channel.empty() + + // + // BCFTOOLS_DEDUP + // + // Deduplicates variants at the same position test + BCFTOOLS_DEDUP( + vcf_ch, + fasta + ) + versions = versions.mix(BCFTOOLS_DEDUP.out.versions) + + // sort vcf + BCFTOOLS_SORT( + BCFTOOLS_DEDUP.out.vcf + ) + + TABIX_TABIX( + BCFTOOLS_SORT.out.vcf + ) + + BCFTOOLS_SORT.out.vcf.join(TABIX_TABIX.out.tbi, by:0) + .set{ch_vcf} + + + emit: + ch_vcf // channel: [ val(meta), [ vcf ], [index] ] + versions // channel: [ versions.yml ] + +} From e04e736e7c9a9a7f83a618752efa0a2b63deeca2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 20 Mar 2024 14:01:29 +0000 Subject: [PATCH 049/292] wittyer added --- conf/modules.config | 3 +- conf/test_hg38.config | 4 +- modules.json | 5 + modules/local/wittyer.nf | 50 --- modules/nf-core/wittyer/README.md | 28 ++ modules/nf-core/wittyer/environment.yml | 7 + modules/nf-core/wittyer/main.nf | 69 ++++ modules/nf-core/wittyer/meta.yml | 66 ++++ modules/nf-core/wittyer/tests/main.nf.test | 100 +++++ .../nf-core/wittyer/tests/main.nf.test.snap | 347 ++++++++++++++++++ modules/nf-core/wittyer/tests/tags.yml | 2 + subworkflows/local/prepare_vcfs_test.nf | 2 +- subworkflows/local/prepare_vcfs_truth.nf | 2 +- subworkflows/local/sv_germline_benchmark.nf | 2 +- 14 files changed, 631 insertions(+), 56 deletions(-) delete mode 100644 modules/local/wittyer.nf create mode 100644 modules/nf-core/wittyer/README.md create mode 100644 modules/nf-core/wittyer/environment.yml create mode 100644 modules/nf-core/wittyer/main.nf create mode 100644 modules/nf-core/wittyer/meta.yml create mode 100644 modules/nf-core/wittyer/tests/main.nf.test create mode 100644 modules/nf-core/wittyer/tests/main.nf.test.snap create mode 100644 modules/nf-core/wittyer/tests/tags.yml diff --git a/conf/modules.config b/conf/modules.config index 1acc629..b6e76c5 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -168,6 +168,7 @@ process { } withName: WITTYER { ext.prefix = {"${params.sample}"} + ext.args = {"-em cts"} publishDir = [ path: {"${params.outdir}/${meta.id}/wittyer_bench"}, pattern: "*{json,vcf.gz.tbi,vcf.gz}", @@ -220,7 +221,7 @@ process { // Don't publish results for these processes // process { - withName: 'BGZIP_TABIX|TABIX_TABIX|TABIX_BGZIPTABIX|RTGTOOLS_FORMAT' { + withName: 'TABIX_TABIX|TABIX_BGZIPTABIX|TABIX_BGZIP|RTGTOOLS_FORMAT' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false diff --git a/conf/test_hg38.config b/conf/test_hg38.config index 0535bfc..ccc7c32 100644 --- a/conf/test_hg38.config +++ b/conf/test_hg38.config @@ -30,7 +30,7 @@ params { // Processes analysis = 'germline' //somatic - method = 'truvari,svanalyzer' // --not working for now : vcfdist + method = 'truvari,svanalyzer,wittyer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. preprocess = "normalization, deduplication" min_sv_size = 50 @@ -38,6 +38,6 @@ params { sample = "HG002" // available samples: SEQC2, HG002 truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - //high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" } diff --git a/modules.json b/modules.json index b19094d..9587834 100644 --- a/modules.json +++ b/modules.json @@ -94,6 +94,11 @@ "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": ["modules"] + }, + "wittyer": { + "branch": "master", + "git_sha": "61f2ea506bd87ef436b0086f91a07abc6035fcd0", + "installed_by": ["modules"] } } }, diff --git a/modules/local/wittyer.nf b/modules/local/wittyer.nf deleted file mode 100644 index 43c0a83..0000000 --- a/modules/local/wittyer.nf +++ /dev/null @@ -1,50 +0,0 @@ -process WITTYER { - tag "$meta.id" - label 'process_single' - - conda "" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker://kubran/wittyer:0.3.3.0' : - 'kubran/wittyer:0.3.3.0' }" - - input: - tuple val(meta),path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) - - output: - tuple val(meta), path("*ConfigFileUsed.json") , emit: config - tuple val(meta), path("*.Stats.json") , emit: report - tuple val(meta), path("*eval.vcf.gz") , emit: bench_vcf - tuple val(meta), path("*eval.vcf.gz.tbi") , emit: bench_vcf_gzi - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def regions = bed ? "-b $bed" : "" - - """ - mkdir bench - dotnet /opt/Wittyer/Wittyer.dll \\ - -i ${vcf} \\ - -t ${truth_vcf} \\ - -o bench \\ - ${regions} \\ - ${args} - - mv bench/Wittyer.ConfigFileUsed.json ${prefix}.ConfigFileUsed.json - mv bench/Wittyer.Stats.json ${prefix}.Stats.json - mv bench/*.vcf.gz ${prefix}.eval.vcf.gz - mv bench/*.vcf.gz.tbi ${prefix}.eval.vcf.gz.tbi - - rm -rf bench - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - wittyer: 0.3.3.0 - END_VERSIONS - """ - -} diff --git a/modules/nf-core/wittyer/README.md b/modules/nf-core/wittyer/README.md new file mode 100644 index 0000000..d2ba0e0 --- /dev/null +++ b/modules/nf-core/wittyer/README.md @@ -0,0 +1,28 @@ +# Updating the docker container and making a new module release + +witty.er is a commercial tool from Illumina. The container provided for the witty.er nf-core module is not provided nor supported by Illumina. Updating the witty.er versions in the container and pushing the update to Dockerhub needs to be done manually. + +1. Navigate to the witty.er github repository. - [witty.er](https://github.com/Illumina/witty.er) +2. Download the latest release. + ```bash + wget https://github.com/Illumina/witty.er/archive/refs/tags/.tar.gz + ``` +3. Uncompress the released package. + ```bash + tar -xvf .tar.gz + ``` +4. Change to the uncompressed directory. +5. Build docker image using provided Dockerfile. + + ```bash + docker build -t wittyer: --platform linux/amd64 . + ``` + +6. Access rights are needed to push the container to the Dockerhub nfcore organization, please ask a core team member to do so. + + ```bash + docker tag wittyer: quay.io/nf-core/wittyer: + docker push quay.io/nf-core/wittyer: + ``` + +7. Make the image public. diff --git a/modules/nf-core/wittyer/environment.yml b/modules/nf-core/wittyer/environment.yml new file mode 100644 index 0000000..f8378df --- /dev/null +++ b/modules/nf-core/wittyer/environment.yml @@ -0,0 +1,7 @@ +name: wittyer +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - tabix diff --git a/modules/nf-core/wittyer/main.nf b/modules/nf-core/wittyer/main.nf new file mode 100644 index 0000000..c2b943f --- /dev/null +++ b/modules/nf-core/wittyer/main.nf @@ -0,0 +1,69 @@ +process WITTYER { + tag "$meta.id" + label 'process_single' + + container "nf-core/wittyer:0.3.3.0" + + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "WITTYER module does not support Conda. Please use Docker / Singularity / Podman instead." + } + + input: + tuple val(meta), path(query_vcf), path(query_vcf_index), path(truth_vcf), path(truth_vcf_index), path(bed) + + output: + tuple val(meta), path("*.json") , emit: report + tuple val(meta), path("*.vcf.gz") , emit: bench_vcf + tuple val(meta), path("*.vcf.gz.tbi") , emit: bench_vcf_tbi + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def regions = bed ? "--includeBed=$bed" : "" + if ("$truth_vcf" == "${prefix}.vcf.gz") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + if ("$query_vcf" == "${prefix}.vcf.gz") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + if ("$query_vcf_index" == "${prefix}.vcf.gz.tbi") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + if ("$query_vcf_index" == "${prefix}.vcf.gz.tbi") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + + // dotnet /opt/Wittyer/Wittyer.dll might need to be replaced with new docker image + """ + mkdir bench + + dotnet /opt/Wittyer/Wittyer.dll \\ + --truthVcf=${truth_vcf} \\ + --inputVcf=${query_vcf} \\ + --outputDirectory=bench \\ + ${regions} \\ + ${args} + + mv bench/Wittyer.Stats.json ${prefix}.json + mv bench/*.vcf.gz ${prefix}.vcf.gz + mv bench/*.vcf.gz.tbi ${prefix}.vcf.gz.tbi + + rm -rf bench + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wittyer: \$(dotnet /opt/Wittyer/Wittyer.dll --version |& sed '1!d ; s/witty.er //') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.json + echo "" | gzip > ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + wittyer: \$(dotnet /opt/Wittyer/Wittyer.dll --version |& sed '1!d ; s/witty.er //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/wittyer/meta.yml b/modules/nf-core/wittyer/meta.yml new file mode 100644 index 0000000..097f90f --- /dev/null +++ b/modules/nf-core/wittyer/meta.yml @@ -0,0 +1,66 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: wittyer +description: A large variant benchmarking tool analogous to hap.py for small variants. +keywords: + - structural-variants + - benchmarking + - vcf +tools: + - wittyer: + description: "Illumina tool for large variant benchmarking" + homepage: "https://github.com/Illumina/witty.er" + documentation: "https://github.com/Illumina/witty.er" + tool_dev_url: "https://github.com/Illumina/witty.er" + licence: ["BSD-2"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - query_vcf: + type: file + description: A VCF with called variants to benchmark against the standard + pattern: "*.{vcf,vcf.gz}" + - query_vcf_index: + type: file + description: The index of the called VCF (optional) + pattern: "*.tbi" + - truth_vcf: + type: file + description: A standard VCF to compare against + pattern: "*.{vcf,vcf.gz}" + - truth_vcf_index: + type: file + description: The index of the standard VCF (optional) + pattern: "*.tbi" + - bed: + type: file + description: A BED file specifying regions to be included in the analysis (optional) + pattern: "*.bed" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - report: + type: file + description: Detailed per-sample-pair, per-svtype, per-bin stats + pattern: "*.json" + - bench_vcf: + type: file + description: Updated query and truth entries merged into one file + pattern: "*.vcf.gz" + - bench_vcf_tbi: + type: file + description: Index of merged query and truth entries VCF file + pattern: "*.vcf.gz.tbi" +authors: + - "@famosab" +maintainers: + - "@famosab" diff --git a/modules/nf-core/wittyer/tests/main.nf.test b/modules/nf-core/wittyer/tests/main.nf.test new file mode 100644 index 0000000..3c23ffe --- /dev/null +++ b/modules/nf-core/wittyer/tests/main.nf.test @@ -0,0 +1,100 @@ +nextflow_process { + + name "Test Process WITTYER" + script "../main.nf" + process "WITTYER" + + tag "modules" + tag "modules_nfcore" + tag "wittyer" + + test("human - simulatedSV - vcf_gz") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['simulated_sv'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv2'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv2_tbi'], checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.bench_vcf).match("bench_vcf") }, + { assert snapshot(process.out.bench_vcf_tbi).match("bench_vcf_tbi") }, + { assert snapshot(process.out.report).match("report") }, + { assert snapshot(process.out.version).match("version") } + ) + } + + } + + test("human - simulatedSV - vcf_gz - bed") { + + when { + process { + """ + input[0] = [ + [ id:'test_bed', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['simulated_sv'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv2'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv2_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['genome']['genome_21_multi_interval_bed'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.bench_vcf).match("bed_bench_vcf") }, + { assert snapshot(process.out.bench_vcf_tbi).match("bed_bench_vcf_tbi") }, + { assert snapshot(process.out.report).match("bed_report") }, + { assert snapshot(process.out.version).match("bed_version") } + ) + } + + } + + test("human - simulatedSV - vcf_gz - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test_stub', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['simulated_sv'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv_tbi'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv2'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['simulated_sv2_tbi'], checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert snapshot(process.out.version).match("stub_version") } + ) + } + + } + +} diff --git a/modules/nf-core/wittyer/tests/main.nf.test.snap b/modules/nf-core/wittyer/tests/main.nf.test.snap new file mode 100644 index 0000000..a25d824 --- /dev/null +++ b/modules/nf-core/wittyer/tests/main.nf.test.snap @@ -0,0 +1,347 @@ +{ + "human - simulatedSV - vcf_gz - bed": { + "content": [ + { + "0": [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.json:md5,c6515ada81b5ccf5aa5b4f1268da2800" + ] + ], + "1": [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.vcf.gz:md5,7e5f24415c80ca986e81be90f831e000" + ] + ], + "2": [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.vcf.gz.tbi:md5,e4de1e1d27208b56f5a7bfbe31542240" + ] + ], + "3": [ + "versions.yml:md5,4a5148f206a3b12f0ebe87e81cedc31a" + ], + "bench_vcf": [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.vcf.gz:md5,7e5f24415c80ca986e81be90f831e000" + ] + ], + "bench_vcf_tbi": [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.vcf.gz.tbi:md5,e4de1e1d27208b56f5a7bfbe31542240" + ] + ], + "report": [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.json:md5,c6515ada81b5ccf5aa5b4f1268da2800" + ] + ], + "versions": [ + "versions.yml:md5,4a5148f206a3b12f0ebe87e81cedc31a" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:41:49.782336703" + }, + "stub_version": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:57.223103062" + }, + "human - simulatedSV - vcf_gz": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.json:md5,fb70eac691c1067167091ab2d3b12de3" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz:md5,ff56c3084a59507362f6b7b7dc46ffdc" + ] + ], + "2": [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz.tbi:md5,b9b448e5f11eebbcfeb9a123e838caa4" + ] + ], + "3": [ + "versions.yml:md5,4a5148f206a3b12f0ebe87e81cedc31a" + ], + "bench_vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz:md5,ff56c3084a59507362f6b7b7dc46ffdc" + ] + ], + "bench_vcf_tbi": [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz.tbi:md5,b9b448e5f11eebbcfeb9a123e838caa4" + ] + ], + "report": [ + [ + { + "id": "test", + "single_end": false + }, + "test.json:md5,fb70eac691c1067167091ab2d3b12de3" + ] + ], + "versions": [ + "versions.yml:md5,4a5148f206a3b12f0ebe87e81cedc31a" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:41:44.466462714" + }, + "bench_vcf": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz:md5,ff56c3084a59507362f6b7b7dc46ffdc" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:47.523573536" + }, + "bench_vcf_tbi": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.vcf.gz.tbi:md5,b9b448e5f11eebbcfeb9a123e838caa4" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:47.532504108" + }, + "report": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.json:md5,fb70eac691c1067167091ab2d3b12de3" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:47.537692046" + }, + "bed_report": { + "content": [ + [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.json:md5,c6515ada81b5ccf5aa5b4f1268da2800" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:52.863563564" + }, + "bed_version": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:52.868467701" + }, + "version": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:47.542666285" + }, + "human - simulatedSV - vcf_gz - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test_stub", + "single_end": false + }, + "test_stub.json:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test_stub", + "single_end": false + }, + "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "2": [ + [ + { + "id": "test_stub", + "single_end": false + }, + "test_stub.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,4a5148f206a3b12f0ebe87e81cedc31a" + ], + "bench_vcf": [ + [ + { + "id": "test_stub", + "single_end": false + }, + "test_stub.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "bench_vcf_tbi": [ + [ + { + "id": "test_stub", + "single_end": false + }, + "test_stub.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "report": [ + [ + { + "id": "test_stub", + "single_end": false + }, + "test_stub.json:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,4a5148f206a3b12f0ebe87e81cedc31a" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:57.215084162" + }, + "bed_bench_vcf": { + "content": [ + [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.vcf.gz:md5,7e5f24415c80ca986e81be90f831e000" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:52.852045028" + }, + "bed_bench_vcf_tbi": { + "content": [ + [ + [ + { + "id": "test_bed", + "single_end": false + }, + "test_bed.vcf.gz.tbi:md5,e4de1e1d27208b56f5a7bfbe31542240" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T09:36:52.857651771" + } +} \ No newline at end of file diff --git a/modules/nf-core/wittyer/tests/tags.yml b/modules/nf-core/wittyer/tests/tags.yml new file mode 100644 index 0000000..177db94 --- /dev/null +++ b/modules/nf-core/wittyer/tests/tags.yml @@ -0,0 +1,2 @@ +wittyer: + - "modules/nf-core/wittyer/**" diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index a0260bf..4ae37a6 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -42,7 +42,7 @@ workflow PREPARE_VCFS_TEST { // PREPARE_VCFS // // Reheader needed to standardize sample names - ch_samples = Channel.of(["samples.txt", params.sample,"_query"]) + ch_samples = Channel.of(["samples.txt", params.sample,"query_"]) .collectFile(newLine:false) vcf_ch.combine(ch_samples) diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 7d02b5b..86371e0 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -34,7 +34,7 @@ workflow PREPARE_VCFS_TRUTH { vcf_ch = BGZIP_TABIX.out.gz_tbi // Reheader needed to standardize sample names - ch_samples = Channel.of(["samples.txt", params.sample,"_truth"]) + ch_samples = Channel.of(["samples.txt", params.sample,"truth_"]) .collectFile(newLine:false) vcf_ch.combine(ch_samples) diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index 1a068fd..f6e97df 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -7,7 +7,7 @@ params.options = [:] include { TRUVARI_PHAB } from '../../modules/local/truvari_phab' addParams( options: params.options ) include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' addParams( options: params.options ) include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' addParams( options: params.options ) -include { WITTYER } from '../../modules/local/wittyer' addParams( options: params.options ) +include { WITTYER } from '../../modules/nf-core/wittyer' addParams( options: params.options ) include { VCFDIST } from '../../modules/local/vcfdist' addParams( options: params.options ) include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon_evaluator' addParams( options: params.options ) From fd5ede1bccf60302c992833ea028d1b7b765f16a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 4 Apr 2024 11:56:33 +0200 Subject: [PATCH 050/292] SURVIVOR SV filters added --- .gitignore | 1 + conf/test.config | 3 ++- nextflow.config | 14 +++++++++++--- nextflow_schema.json | 20 +++++++++++++++++++- subworkflows/local/prepare_vcfs_test.nf | 9 ++++++--- subworkflows/local/sv_variant_filtering.nf | 8 ++++---- 6 files changed, 43 insertions(+), 12 deletions(-) diff --git a/.gitignore b/.gitignore index 6e0bd82..d33a105 100644 --- a/.gitignore +++ b/.gitignore @@ -9,4 +9,5 @@ testing* test_small*/ test_sv*/ test/ +null/* diff --git a/conf/test.config b/conf/test.config index bf9fcb7..168f3fb 100644 --- a/conf/test.config +++ b/conf/test.config @@ -31,7 +31,8 @@ params { method = 'truvari,svanalyzer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. preprocess = "normalization, deduplication, prepy" - min_sv_size = 50 + //min_sv_size = 50 + max_sv_size = 400 truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" } diff --git a/nextflow.config b/nextflow.config index a27cd87..b5a7f22 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,12 +13,18 @@ params { // Input options input = null outdir = "results" + + // truth vcf and high confidence bed files + // Small variant benchmarking: truth_small = null high_conf_small = null + // Structural variant benchmarking truth_sv = null high_conf_sv = null + // CNV variant benchmarking truth_cnv = null high_conf_cnv = null + // Standardization tool config file svync_yaml = null // Preprocess spesific parameters @@ -29,14 +35,16 @@ params { similarity = null // Benchmarking method - method = 'truvari,svanalyzer,happy,rtgtools' // --not working for now : wittyer, vcfdist + method = 'truvari,svanalyzer,happy,rtgtools,wittyer' // --not working for now : vcfdist + // Filtering parameters // minsize effects both truvari and variantbenchmarkingmark in different ways! svbechmark filters both base and comp calls // although with truvari it is possible to filter only base, which makes more sense to me! // I will use this parameter to filter SVs before benchmarking! min_sv_size = 50 - - // TODO: VCF filtering parameters? + max_sv_size = -1 + min_allele_freq = -1 + min_num_reads = -1 // pass_only // MAPQ // QUAL diff --git a/nextflow_schema.json b/nextflow_schema.json index 86be229..e2f7619 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -125,7 +125,25 @@ "min_sv_size": { "type": "integer", "format": "directory-path", - "description": "", + "description": "Maximum SV size of variants to benchmark, 0 to disable , Default:50", + "fa_icon": "fas fa-folder-open" + }, + "max_sv_size": { + "type": "integer", + "format": "directory-path", + "description": "Maximum SV size of variants to benchmark, -1 to disable , Default:-1", + "fa_icon": "fas fa-folder-open" + }, + "min_allele_freq": { + "type": "number", + "format": "directory-path", + "description": "Minimum Alele Frequency of variants to benchmark, Use -1 to disable , Default:-1", + "fa_icon": "fas fa-folder-open" + }, + "min_num_reads": { + "type": "integer", + "format": "directory-path", + "description": "Minimum number of read supporting variants to benchmarkUse, -1 to disable , Default:-1", "fa_icon": "fas fa-folder-open" }, "email": { diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 4ae37a6..d24baf2 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -104,8 +104,11 @@ workflow PREPARE_VCFS_TEST { out_vcf_ch = Channel.empty() - if (params.min_sv_size > 0){ - + if (params.min_sv_size > 0 | params.max_sv_size != -1 | params.min_allele_freq != -1 | params.min_num_reads != -1){ + // + // SUBWORKFLOW: SV_VARIANT_FILTERING + // + // Filters SVs with given paramaters SV_VARIANT_FILTERING( vcf.sv ) @@ -116,7 +119,7 @@ workflow PREPARE_VCFS_TEST { } if (params.preprocess.contains("deduplication")){ // - // VCF_VARIANT_DEDUPLICATION + // SUBWORKFLOW: VCF_VARIANT_DEDUPLICATION // // Deduplicates variants at the same position test VCF_VARIANT_DEDUPLICATION( diff --git a/subworkflows/local/sv_variant_filtering.nf b/subworkflows/local/sv_variant_filtering.nf index 96e30a8..0d9c628 100644 --- a/subworkflows/local/sv_variant_filtering.nf +++ b/subworkflows/local/sv_variant_filtering.nf @@ -1,5 +1,5 @@ // -// SV_VARIANT_FILTERING: filter sv variants using survivor +// SV_VARIANT_FILTERING: Filter SV using survivor and bcftools // params.options = [:] @@ -29,9 +29,9 @@ workflow SV_VARIANT_FILTERING { SURVIVOR_FILTER( TABIX_BGZIP.out.output.map{it -> tuple( it[0], it[1],[])}, params.min_sv_size, - -1, - -1, - -1 + params.max_sv_size, + params.min_allele_freq, + params.min_num_reads ) versions = versions.mix(SURVIVOR_FILTER.out.versions) From 0a2a3b9f161322ac58a30d975388b6868c7a5d83 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 4 Apr 2024 14:23:09 +0000 Subject: [PATCH 051/292] bcftools filter is added to be used with expressions for all type of variants --- conf/modules.config | 30 ++++-- conf/test.config | 10 +- conf/test_small.config | 5 +- .../nf-core/bcftools/filter/environment.yml | 7 ++ modules/nf-core/bcftools/filter/main.nf | 67 ++++++++++++ modules/nf-core/bcftools/filter/meta.yml | 44 ++++++++ .../bcftools/filter/tests/main.nf.test | 82 ++++++++++++++ .../bcftools/filter/tests/main.nf.test.snap | 101 ++++++++++++++++++ .../bcftools/filter/tests/nextflow.config | 3 + .../nf-core/bcftools/filter/tests/tags.yml | 2 + nextflow.config | 6 +- nextflow_schema.json | 14 ++- subworkflows/local/prepare_vcfs_test.nf | 49 ++++----- subworkflows/local/prepare_vcfs_truth.nf | 16 ++- subworkflows/local/sv_variant_filtering.nf | 46 -------- subworkflows/local/vcf_variant_filtering.nf | 83 ++++++++++++++ 16 files changed, 470 insertions(+), 95 deletions(-) create mode 100644 modules/nf-core/bcftools/filter/environment.yml create mode 100644 modules/nf-core/bcftools/filter/main.nf create mode 100644 modules/nf-core/bcftools/filter/meta.yml create mode 100644 modules/nf-core/bcftools/filter/tests/main.nf.test create mode 100644 modules/nf-core/bcftools/filter/tests/main.nf.test.snap create mode 100644 modules/nf-core/bcftools/filter/tests/nextflow.config create mode 100644 modules/nf-core/bcftools/filter/tests/tags.yml delete mode 100644 subworkflows/local/sv_variant_filtering.nf create mode 100644 subworkflows/local/vcf_variant_filtering.nf diff --git a/conf/modules.config b/conf/modules.config index b6e76c5..f62340b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -27,27 +27,24 @@ process { ] } withName: "BCFTOOLS_NORM" { - ext.prefix = {"${params.sample}"} + ext.prefix = { vcf.baseName - ".vcf" + ".norm"} ext.args = {"--output-type z -N -m-any -c s" } - ext.prefix = {"${meta.id}_norm"} publishDir = [ path: { "${params.outdir}/test" }, enabled: false ] } withName: "BCFTOOLS_DEDUP" { - ext.prefix = {"${params.sample}"} + ext.prefix = { vcf.baseName - ".vcf" + ".dedup"} ext.args = {"--output-type z --rm-du exact -c s" } - ext.prefix = {"${meta.id}_dedup"} publishDir = [ path: { "${params.outdir}/test" }, enabled: false ] } withName: "BCFTOOLS_SORT" { - ext.prefix = {"${params.sample}"} + ext.prefix = { vcf.baseName - ".vcf" + ".sort"} ext.args = {"--output-type z" } - ext.prefix = {"${meta.id}_sort"} publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz}", @@ -79,9 +76,8 @@ process { ] } withName: BCFTOOLS_VIEW { - ext.prefix = {"${params.sample}"} - ext.args2 = {"--output-type z" } - ext.prefix = {"${meta.id}_filter"} + ext.prefix = { vcf.baseName - ".vcf" } + ext.args2 = {"--output-type z" } publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", @@ -96,8 +92,17 @@ process { mode: params.publish_dir_mode ] } + withName: BCFTOOLS_FILTER { + ext.prefix = { vcf.baseName - ".vcf" + ".filter"} + ext.args = {"--output-type v --$params.variant_filtering '$params.expression'" } + publishDir = [ + path: {"${params.outdir}/${meta.id}/preprocess/"}, + pattern: "*{.vcf}", + mode: params.publish_dir_mode + ] + } withName: SURVIVOR_FILTER { - ext.prefix = {"${params.sample}.filter"} + ext.prefix = { vcf_file.baseName - ".vcf" + ".filter"} publishDir = [ path: {"${params.outdir}/${meta.id}/preprocess"}, pattern: "*{.vcf}", @@ -113,7 +118,6 @@ process { ] } withName: "TRUVARI_PHAB" { - ext.prefix = {"${params.sample}"} ext.prefix = {"${meta.id}.harm"} publishDir = [ path: {"${params.outdir}/${meta.id}/truvari_phab/"}, @@ -124,6 +128,7 @@ process { withName: "RTGTOOLS_VCFEVAL" { ext.prefix = {"${params.sample}"} //ext.args = {"--ref-overlap --all-record --output-mode ga4gh"} + ext.when = { params.method.split(',').contains('rtgtools') } publishDir = [ path: {"${params.outdir}/${meta.id}/rtgtools_bench"}, pattern: "*{.vcf.gz,vcf.gz.tbi,tsv.gz,txt}", @@ -151,6 +156,7 @@ process { withName: "TRUVARI_BENCH" { ext.prefix = {"${params.sample}"} ext.args = {"--pctsize 0.5 --pctovl 0.5 --refdist 1000 --pick ac"} + ext.when = { params.method.split(',').contains('truvari') } publishDir = [ path: {"${params.outdir}/${meta.id}/truvari_bench"}, pattern: "*{.vcf.gz,vcf.gz.tbi,json}", @@ -160,6 +166,7 @@ process { withName: SVANALYZER_SVBENCHMARK { ext.prefix = {"${params.sample}"} ext.args = {"-normshift 0.3 –normdist 0.3 –normsizediff 0.3"} + ext.when = { params.method.split(',').contains('svanalyzer') } publishDir = [ path: {"${params.outdir}/${meta.id}/svanalyzer_bench"}, pattern: "*{.vcf,distances,log,report}", @@ -169,6 +176,7 @@ process { withName: WITTYER { ext.prefix = {"${params.sample}"} ext.args = {"-em cts"} + ext.when = { params.method.split(',').contains('wittyer') } publishDir = [ path: {"${params.outdir}/${meta.id}/wittyer_bench"}, pattern: "*{json,vcf.gz.tbi,vcf.gz}", diff --git a/conf/test.config b/conf/test.config index 168f3fb..e648da2 100644 --- a/conf/test.config +++ b/conf/test.config @@ -30,9 +30,13 @@ params { analysis = 'germline' method = 'truvari,svanalyzer' // --not working for now : vcfdist similarity = 0 // determines the sequence similarity level in benchmarking. - preprocess = "normalization, deduplication, prepy" - //min_sv_size = 50 - max_sv_size = 400 + preprocess = "normalization,deduplication,prepy" + + //// variant filtering options + min_sv_size = 50 + // Bcftools filter options, to include the expression use include true with the expression, to exclude keep include off. To disable filtering turn filter_expr to null. + variant_filtering = "include" // null, include, exclude + expression = 'FILTER="PASS"' truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" } diff --git a/conf/test_small.config b/conf/test_small.config index a64ed2b..3915975 100644 --- a/conf/test_small.config +++ b/conf/test_small.config @@ -28,9 +28,10 @@ params { // Processes analysis = 'germline' - method = 'rtgtools,happy' // + method = 'rtgtools' // preprocess = "normalization,deduplication,prepy" - //min_sv_size = 50 + variant_filtering = "include" // null, include, exclude + expression = 'FILTER="."' truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" diff --git a/modules/nf-core/bcftools/filter/environment.yml b/modules/nf-core/bcftools/filter/environment.yml new file mode 100644 index 0000000..b269875 --- /dev/null +++ b/modules/nf-core/bcftools/filter/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_filter +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.18 diff --git a/modules/nf-core/bcftools/filter/main.nf b/modules/nf-core/bcftools/filter/main.nf new file mode 100644 index 0000000..cc9a236 --- /dev/null +++ b/modules/nf-core/bcftools/filter/main.nf @@ -0,0 +1,67 @@ +process BCFTOOLS_FILTER { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': + 'biocontainers/bcftools:1.18--h8b25389_0' }" + + input: + tuple val(meta), path(vcf) + + output: + tuple val(meta), path("*.${extension}"), emit: vcf + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + if ("$vcf" == "${prefix}.${extension}") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + + """ + bcftools filter \\ + --output ${prefix}.${extension} \\ + --threads ${task.cpus} \\ + $args \\ + $vcf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : + args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : + args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : + args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : + "vcf" + + if ("$vcf" == "${prefix}.${extension}") error "Input and output names are the same, set prefix in module configuration to disambiguate!" + + def create_file = extension.endsWith(".gz") ? "echo '' | gzip > ${prefix}.${extension}" : "touch ${prefix}.${extension}" + + """ + ${create_file} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/bcftools/filter/meta.yml b/modules/nf-core/bcftools/filter/meta.yml new file mode 100644 index 0000000..deb93b1 --- /dev/null +++ b/modules/nf-core/bcftools/filter/meta.yml @@ -0,0 +1,44 @@ +name: bcftools_filter +description: Filters VCF files +keywords: + - variant calling + - filtering + - VCF +tools: + - filter: + description: | + Apply fixed-threshold filters to VCF files. + homepage: http://samtools.github.io/bcftools/bcftools.html + documentation: http://www.htslib.org/doc/bcftools.html + doi: 10.1093/bioinformatics/btp352 + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF input file + pattern: "*.{vcf,bcf,vcf.gz,bcf.gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF filtered output file + pattern: "*.{vcf,bcf,vcf.gz,bcf.gz}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/bcftools/filter/tests/main.nf.test b/modules/nf-core/bcftools/filter/tests/main.nf.test new file mode 100644 index 0000000..eaf100e --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/main.nf.test @@ -0,0 +1,82 @@ +nextflow_process { + + name "Test Process BCFTOOLS_FILTER" + script "../main.nf" + process "BCFTOOLS_FILTER" + + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/filter" + + config "./nextflow.config" + + test("sarscov2 - vcf") { + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("vcf") } + ) + } + + } + + test("sarscov2 - vcf - bcf output") { + + when { + process { + """ + input[0] = [ + [id:"bcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("bcf output") } + ) + } + + } + + test("sarscov2 - vcf - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("vcf - stub") } + ) + } + + } + +} diff --git a/modules/nf-core/bcftools/filter/tests/main.nf.test.snap b/modules/nf-core/bcftools/filter/tests/main.nf.test.snap new file mode 100644 index 0000000..f8e17aa --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/main.nf.test.snap @@ -0,0 +1,101 @@ +{ + "vcf": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_test" + }, + "vcf_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + ], + "vcf": [ + [ + { + "id": "vcf_test" + }, + "vcf_test.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.02.0" + }, + "timestamp": "2024-03-27T16:57:32.940161987" + }, + "bcf output": { + "content": [ + { + "0": [ + [ + { + "id": "bcf_test" + }, + "bcf_test.bcf.gz:md5,c8a304c8d2892039201154153c8cd536" + ] + ], + "1": [ + "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + ], + "vcf": [ + [ + { + "id": "bcf_test" + }, + "bcf_test.bcf.gz:md5,c8a304c8d2892039201154153c8cd536" + ] + ], + "versions": [ + "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.02.0" + }, + "timestamp": "2024-03-27T16:45:14.586866398" + }, + "vcf - stub": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_test" + }, + "vcf_test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + ], + "vcf": [ + [ + { + "id": "vcf_test" + }, + "vcf_test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.02.0" + }, + "timestamp": "2024-03-27T17:05:52.80837892" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/filter/tests/nextflow.config b/modules/nf-core/bcftools/filter/tests/nextflow.config new file mode 100644 index 0000000..4e960c8 --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = {"--no-version${meta.id == 'bcf_test' ? ' --output-type b' : ' --output-type z'}"} +} diff --git a/modules/nf-core/bcftools/filter/tests/tags.yml b/modules/nf-core/bcftools/filter/tests/tags.yml new file mode 100644 index 0000000..d5e0108 --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/filter: + - "modules/nf-core/bcftools/filter/**" diff --git a/nextflow.config b/nextflow.config index b5a7f22..dd1cb1b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -45,9 +45,9 @@ params { max_sv_size = -1 min_allele_freq = -1 min_num_reads = -1 - // pass_only - // MAPQ - // QUAL + // use bcftools expressions https://samtools.github.io/bcftools/bcftools.html#expressions together with variant_filtering parameter! + variant_filtering = null // null, include, exclude + expression = null // References diff --git a/nextflow_schema.json b/nextflow_schema.json index e2f7619..39700cf 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -143,7 +143,19 @@ "min_num_reads": { "type": "integer", "format": "directory-path", - "description": "Minimum number of read supporting variants to benchmarkUse, -1 to disable , Default:-1", + "description": "Minimum number of read supporting variants to benchmark, Use, -1 to disable , Default:-1", + "fa_icon": "fas fa-folder-open" + }, + "variant_filtering": { + "type": "string", + "format": "directory-path", + "description": "Use either exclude or include to enable variant filtering using bcftools expressions, Default:null", + "fa_icon": "fas fa-folder-open" + }, + "expression": { + "type": "string", + "format": "directory-path", + "description": "Use bcftools expressions here https://samtools.github.io/bcftools/bcftools.html#expressions. This must be coupled with variant_expression, Default:null", "fa_icon": "fas fa-folder-open" }, "email": { diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index d24baf2..f59eb33 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -3,14 +3,13 @@ // params.options = [:] - -include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) -include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) -include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' addParams( options: params.options ) -include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' addParams( options: params.options ) -include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) -include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' addParams( options: params.options ) -include { SV_VARIANT_FILTERING } from '../local/sv_variant_filtering' addParams( options: params.options ) +include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' addParams( options: params.options ) +include { VCF_VARIANT_FILTERING } from '../local/vcf_variant_filtering' addParams( options: params.options ) +include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' addParams( options: params.options ) +include { BCFTOOLS_VIEW } from '../../modules/local/bcftools_view' addParams( options: params.options ) +include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' addParams( options: params.options ) +include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' addParams( options: params.options ) +include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' addParams( options: params.options ) include { TABIX_TABIX as TABIX_TABIX_1 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) include { TABIX_TABIX as TABIX_TABIX_3 } from '../../modules/nf-core/tabix/tabix' addParams( options: params.options ) include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_1 } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) @@ -42,13 +41,17 @@ workflow PREPARE_VCFS_TEST { // PREPARE_VCFS // // Reheader needed to standardize sample names - ch_samples = Channel.of(["samples.txt", params.sample,"query_"]) + ch_samples = Channel.of(["samples.txt", params.sample,"query"]) .collectFile(newLine:false) vcf_ch.combine(ch_samples) .map{it -> tuple( it[0], it[1],[],it[3])} .set{input_ch} + // + // BCFTOOLS_REHEADER + // + // Add "query" to test sample BCFTOOLS_REHEADER_TEST( input_ch, fai @@ -58,6 +61,7 @@ workflow PREPARE_VCFS_TEST { TABIX_BGZIPTABIX_1( BCFTOOLS_REHEADER_TEST.out.vcf ) + versions = versions.mix(TABIX_BGZIPTABIX_1.out.versions) vcf_ch = TABIX_BGZIPTABIX_1.out.gz_tbi if (params.preprocess.contains("filter_contigs")){ @@ -73,6 +77,7 @@ workflow PREPARE_VCFS_TEST { TABIX_BGZIPTABIX_2( BCFTOOLS_VIEW.out.vcf ) + versions = versions.mix(TABIX_BGZIPTABIX_2.out.versions) vcf_ch = TABIX_BGZIPTABIX_2.out.gz_tbi } @@ -90,33 +95,24 @@ workflow PREPARE_VCFS_TEST { TABIX_TABIX_1( BCFTOOLS_NORM.out.vcf ) - + versions = versions.mix(TABIX_TABIX_1.out.versions) BCFTOOLS_NORM.out.vcf.join(TABIX_TABIX_1.out.tbi, by:0) .set{vcf_ch} } - vcf_ch.branch{ - sv: it[0].vartype == "sv" - small: it[0].vartype == "small" - cnv: it[0].vartype == "cnv" - other: false} - .set{vcf} - - out_vcf_ch = Channel.empty() - if (params.min_sv_size > 0 | params.max_sv_size != -1 | params.min_allele_freq != -1 | params.min_num_reads != -1){ + if (params.variant_filtering != null | params.min_sv_size > 0 | params.max_sv_size != -1 | params.min_allele_freq != -1 | params.min_num_reads != -1 ){ // - // SUBWORKFLOW: SV_VARIANT_FILTERING + // SUBWORKFLOW: VCF_VARIANT_FILTERING // // Filters SVs with given paramaters - SV_VARIANT_FILTERING( - vcf.sv + VCF_VARIANT_FILTERING( + vcf_ch ) - out_vcf_ch = out_vcf_ch.mix(SV_VARIANT_FILTERING.out.vcf_ch) - out_vcf_ch = out_vcf_ch.mix(vcf.small) - out_vcf_ch = out_vcf_ch.mix(vcf.cnv) - vcf_ch = out_vcf_ch + vcf_ch = VCF_VARIANT_FILTERING.out.vcf_ch + versions = versions.mix(VCF_VARIANT_FILTERING.out.versions) } + if (params.preprocess.contains("deduplication")){ // // SUBWORKFLOW: VCF_VARIANT_DEDUPLICATION @@ -127,6 +123,7 @@ workflow PREPARE_VCFS_TEST { fasta ) vcf_ch = VCF_VARIANT_DEDUPLICATION.out.ch_vcf + versions = versions.mix(VCF_VARIANT_DEDUPLICATION.out.versions) } vcf_ch.branch{ diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 86371e0..5378583 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -34,19 +34,26 @@ workflow PREPARE_VCFS_TRUTH { vcf_ch = BGZIP_TABIX.out.gz_tbi // Reheader needed to standardize sample names - ch_samples = Channel.of(["samples.txt", params.sample,"truth_"]) + ch_samples = Channel.of(["samples.txt", params.sample,"truth"]) .collectFile(newLine:false) vcf_ch.combine(ch_samples) .map{it -> tuple( it[0], it[1],[],it[3])} .set{input_ch} - + // + // BCFTOOLS_REHEADER + // + // Add "truth" to test sample BCFTOOLS_REHEADER_TRUTH( input_ch, fai ) versions = versions.mix(BCFTOOLS_REHEADER_TRUTH.out.versions) + // + // TABIX_BGZIPTABIX + // + // bgzip and index vcf file TABIX_BGZIPTABIX( BCFTOOLS_REHEADER_TRUTH.out.vcf ) @@ -63,7 +70,10 @@ workflow PREPARE_VCFS_TRUTH { fasta ) versions = versions.mix(BCFTOOLS_NORM.out.versions) - + // + // TABIX_BGZIPTABIX + // + // ndex vcf file TABIX_TABIX( BCFTOOLS_NORM.out.vcf ) diff --git a/subworkflows/local/sv_variant_filtering.nf b/subworkflows/local/sv_variant_filtering.nf deleted file mode 100644 index 0d9c628..0000000 --- a/subworkflows/local/sv_variant_filtering.nf +++ /dev/null @@ -1,46 +0,0 @@ -// -// SV_VARIANT_FILTERING: Filter SV using survivor and bcftools -// - -params.options = [:] - -include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) -include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) - - -workflow SV_VARIANT_FILTERING { - take: - vcf_ch // channel: [val(meta),vcf] - - main: - - versions=Channel.empty() - - TABIX_BGZIP( - vcf_ch.map{it -> tuple( it[0], it[1])} - ) - versions = versions.mix(TABIX_BGZIP.out.versions) - - // - // MODULE: SURVIVOR_FILTER - // - // filters out smaller SVs than min_sv_size - SURVIVOR_FILTER( - TABIX_BGZIP.out.output.map{it -> tuple( it[0], it[1],[])}, - params.min_sv_size, - params.max_sv_size, - params.min_allele_freq, - params.min_num_reads - ) - versions = versions.mix(SURVIVOR_FILTER.out.versions) - - TABIX_BGZIPTABIX( - SURVIVOR_FILTER.out.vcf - ) - vcf_ch = TABIX_BGZIPTABIX.out.gz_tbi - - emit: - vcf_ch - versions -} diff --git a/subworkflows/local/vcf_variant_filtering.nf b/subworkflows/local/vcf_variant_filtering.nf new file mode 100644 index 0000000..efa98c1 --- /dev/null +++ b/subworkflows/local/vcf_variant_filtering.nf @@ -0,0 +1,83 @@ +// +// VCF_VARIANT_FILTERING: Filter SV using survivor and bcftools +// + +params.options = [:] + +include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' addParams( options: params.options ) +include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' addParams( options: params.options ) +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' addParams( options: params.options ) +include { BCFTOOLS_FILTER } from '../../modules/nf-core/bcftools/filter' addParams( options: params.options ) + + +workflow VCF_VARIANT_FILTERING { + take: + vcf_ch // channel: [val(meta),vcf.gz, tbi] + + main: + + versions=Channel.empty() + + // + // TABIX_BGZIP + // + // unzip vcf file, required for survivor filter + TABIX_BGZIP( + vcf_ch + ) + versions = versions.mix(TABIX_BGZIP.out.versions) + vcf_ch = TABIX_BGZIP.out.output + + if(params.variant_filtering != null & params.expression != null){ + // + // BCFTOOLS_FILTER + // + // filter vcf files using bcftools expressions + BCFTOOLS_FILTER( + vcf_ch + ) + versions = versions.mix(BCFTOOLS_FILTER.out.versions) + vcf_ch = BCFTOOLS_FILTER.out.vcf + } + + out_vcf_ch = Channel.empty() + + if(params.min_sv_size > 0 | params.max_sv_size != -1 | params.min_allele_freq != -1 | params.min_num_reads != -1){ + vcf_ch.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{vcf} + + // + // MODULE: SURVIVOR_FILTER + // + // filters out smaller SVs than min_sv_size, only applicable to SV files + SURVIVOR_FILTER( + vcf.sv.map{it -> tuple( it[0], it[1],[])}, + params.min_sv_size, + params.max_sv_size, + params.min_allele_freq, + params.min_num_reads + ) + versions = versions.mix(SURVIVOR_FILTER.out.versions) + + out_vcf_ch = out_vcf_ch.mix(SURVIVOR_FILTER.out.vcf) + out_vcf_ch = out_vcf_ch.mix(vcf.small) + out_vcf_ch = out_vcf_ch.mix(vcf.cnv) + vcf_ch = out_vcf_ch + } + // + // TABIX_BGZIPTABIX + // + // zip and index vcf files + TABIX_BGZIPTABIX( + vcf_ch + ) + vcf_ch = TABIX_BGZIPTABIX.out.gz_tbi + + emit: + vcf_ch + versions +} From 59d44939790ac9a432f3edcf8a34342ab47a7a90 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 24 Apr 2024 10:03:10 +0000 Subject: [PATCH 052/292] sv + small analysis implemented --- .gitignore | 1 + assets/samplesheet_full.csv | 6 +++ conf/modules.config | 2 +- conf/test_full.config | 19 ++++++- conf/test_hg38.config | 6 ++- conf/test_small.config | 4 +- workflows/variantbenchmarking.nf | 85 ++++++++++++++++++++++++++------ 7 files changed, 101 insertions(+), 22 deletions(-) create mode 100644 assets/samplesheet_full.csv diff --git a/.gitignore b/.gitignore index d33a105..8f071fd 100644 --- a/.gitignore +++ b/.gitignore @@ -10,4 +10,5 @@ test_small*/ test_sv*/ test/ null/* +results*/* diff --git a/assets/samplesheet_full.csv b/assets/samplesheet_full.csv new file mode 100644 index 0000000..3123ffd --- /dev/null +++ b/assets/samplesheet_full.csv @@ -0,0 +1,6 @@ +test_vcf,caller,vartype +https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz,haplotypecaller,small +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.chr21.vcf.gz,lumpy,sv +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,sv +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,sv +https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,sv diff --git a/conf/modules.config b/conf/modules.config index f62340b..23add12 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -229,7 +229,7 @@ process { // Don't publish results for these processes // process { - withName: 'TABIX_TABIX|TABIX_BGZIPTABIX|TABIX_BGZIP|RTGTOOLS_FORMAT' { + withName: 'TABIX_TABIX|TABIX_BGZIPTABIX|TABIX_BGZIP|RTGTOOLS_FORMAT|BGZIP_TABIX' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false diff --git a/conf/test_full.config b/conf/test_full.config index 57fba2a..3736fb8 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -17,8 +17,23 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + // Input data + sample = "HG002" + input = "assets/samplesheet_full.csv" // Genome references - genome = 'R64-1-1' + genome = 'hg38' + + // Processes + analysis = 'germline' + method = 'rtgtools,happy,truvari,svanalyzer,wittyer' // + preprocess = "normalization,deduplication,prepy" + variant_filtering = "include" // null, include, exclude + expression = 'FILTER="."' + + truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" + truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + } diff --git a/conf/test_hg38.config b/conf/test_hg38.config index ccc7c32..8f117fa 100644 --- a/conf/test_hg38.config +++ b/conf/test_hg38.config @@ -5,8 +5,8 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile test, --outdir -variantbenchmarking + nextflow run nf-core/benchmark -profile -config conf/test_hg38.config --outdir + ---------------------------------------------------------------------------------------- */ @@ -34,6 +34,8 @@ params { similarity = 0 // determines the sequence similarity level in benchmarking. preprocess = "normalization, deduplication" min_sv_size = 50 + variant_filtering = "include" // null, include, exclude + expression = 'FILTER="PASS"' sample = "HG002" // available samples: SEQC2, HG002 diff --git a/conf/test_small.config b/conf/test_small.config index 3915975..1d6fed1 100644 --- a/conf/test_small.config +++ b/conf/test_small.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, --outdir + nextflow run nf-core/variantbenchmarking -profile -config conf/test_small.config --outdir ---------------------------------------------------------------------------------------- */ @@ -28,7 +28,7 @@ params { // Processes analysis = 'germline' - method = 'rtgtools' // + method = 'rtgtools,happy' // preprocess = "normalization,deduplication,prepy" variant_filtering = "include" // null, include, exclude expression = 'FILTER="."' diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index d99a4a7..138a8e4 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -47,6 +47,7 @@ workflow VARIANTBENCHMARKING { ch_multiqc_files = Channel.empty() truth_ch = Channel.empty() high_conf_ch = Channel.empty() + bench_ch = Channel.empty() // check mandatory parameters println(params.fasta) @@ -139,45 +140,99 @@ workflow VARIANTBENCHMARKING { ) ch_versions = ch_versions.mix(REPORT_VCF_STATISTICS.out.versions) + PREPARE_VCFS_TEST.out.vcf_ch.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{test} + + PREPARE_VCFS_TRUTH.out.vcf_ch.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{truth} + high_conf_ch.branch{ + sv: it[0].vartype == "sv" + small: it[0].vartype == "small" + cnv: it[0].vartype == "cnv" + other: false} + .set{high_conf} // prepare benchmark set - if (params.high_conf_small || params.high_conf_sv || params.high_conf_cnv ){ - PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch) - .combine(high_conf_ch) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} - .set{bench_ch} - }else{ - PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} - .set{bench_ch} - } + if(params.truth_small){ + if(params.high_conf_small){ + test.small.combine(truth.small) + .combine(high_conf.small) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} + .set{bench} + bench_ch = bench_ch.mix(bench) + } + else{ + test.small.combine(truth.small) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} + .set{bench} + bench_ch = bench_ch.mix(bench) + } + } + if(params.truth_sv){ + if(params.high_conf_sv){ + test.sv.combine(truth.sv) + .combine(high_conf.sv) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} + .set{bench} + bench_ch = bench_ch.mix(bench) + } + else{ + test.sv.combine(truth.sv) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} + .set{bench} + bench_ch = bench_ch.mix(bench) + } + } + if(params.truth_cnv){ + if(params.high_conf_cnv){ + test.cnv.combine(truth.cnv) + .combine(high_conf.cnv) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} + .set{bench} + bench_ch = bench_ch.mix(bench) + } + else{ + test.cnv.combine(truth.cnv) + .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} + .set{bench} + bench_ch = bench_ch.mix(bench) + } + } + bench_ch.view() // BENCHMARKS bench_ch.branch{ sv: it[0].vartype == "sv" small: it[0].vartype == "small" cnv: it[0].vartype == "cnv" other: false} - .set{input} + .set{bench_input} // // SUBWORKFLOW: SMALL_GERMLINE_BENCHMARK // //Benchmarking spesific to germline samples SMALL_GERMLINE_BENCHMARK( - input.small, + bench_input.small, fasta, fai ) ch_versions = ch_versions.mix(SMALL_GERMLINE_BENCHMARK.out.versions) - // // SUBWORKFLOW: SV_GERMLINE_BENCHMARK // //Benchmarking spesific to germline samples SV_GERMLINE_BENCHMARK( - input.sv, + bench_input.sv, fasta, fai ) ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) @@ -187,7 +242,7 @@ workflow VARIANTBENCHMARKING { // SOMATIC VARIANT BENCHMARKING SOMATIC_BENCHMARK( - bench_ch, + bench_input, fasta, fai ) From 4bd8ab4c09963c3747d64a978aaa63d2ff8f4476 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 24 Apr 2024 12:12:51 +0200 Subject: [PATCH 053/292] Update workflows/variantbenchmarking.nf Co-authored-by: Maxime U Garcia --- workflows/variantbenchmarking.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 138a8e4..8783597 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -166,7 +166,7 @@ workflow VARIANTBENCHMARKING { if(params.high_conf_small){ test.small.combine(truth.small) .combine(high_conf.small) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} + .map{it -> tuple(it[0], it[1], it[2], it[4], it[5], it[7])} .set{bench} bench_ch = bench_ch.mix(bench) } From fb09977e115e51fac0d3790f755e22c6648a6246 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 24 Apr 2024 10:37:51 +0000 Subject: [PATCH 054/292] fix spaces --- subworkflows/local/prepare_vcfs_truth.nf | 2 +- subworkflows/local/small_germline_benchmark.nf | 4 ++-- subworkflows/local/sv_germline_benchmark.nf | 2 +- subworkflows/local/vcf_variant_filtering.nf | 2 +- workflows/variantbenchmarking.nf | 10 +++++----- 5 files changed, 10 insertions(+), 10 deletions(-) diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 5378583..cf2aeb8 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -38,7 +38,7 @@ workflow PREPARE_VCFS_TRUTH { .collectFile(newLine:false) vcf_ch.combine(ch_samples) - .map{it -> tuple( it[0], it[1],[],it[3])} + .map{it -> tuple( it[0], it[1], [], it[3])} .set{input_ch} // // BCFTOOLS_REHEADER diff --git a/subworkflows/local/small_germline_benchmark.nf b/subworkflows/local/small_germline_benchmark.nf index 3f1439c..12ac411 100644 --- a/subworkflows/local/small_germline_benchmark.nf +++ b/subworkflows/local/small_germline_benchmark.nf @@ -31,7 +31,7 @@ workflow SMALL_GERMLINE_BENCHMARK { // MODULE: RTGTOOLS_VCFEVAL // RTGTOOLS_VCFEVAL( - input_ch.map { it -> tuple(it[0],it[3], it[4], it[1], it[2], it[5], []) }, + input_ch.map { it -> tuple(it[0], it[3], it[4], it[1], it[2], it[5], []) }, RTGTOOLS_FORMAT.out.sdf ) versions = versions.mix(RTGTOOLS_VCFEVAL.out.versions) @@ -40,7 +40,7 @@ workflow SMALL_GERMLINE_BENCHMARK { if (params.method.contains('happy')){ HAPPY_HAPPY( - input_ch.map { it -> tuple(it[0],it[3], it[1], it[5], []) }, + input_ch.map { it -> tuple(it[0], it[3], it[1], it[5], []) }, fasta, fai, [[],[]], diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index f6e97df..ba8b942 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -92,7 +92,7 @@ workflow SV_GERMLINE_BENCHMARK { // //https://github.com/adamewing/bamsurgeon/blob/master/scripts/evaluator.py BAMSURGEON_EVALUATOR( - input_ch.map{it -> tuple(it[0],it[1], it[2], it[3], it[4], it[5])}, + input_ch.map{it -> tuple(it[0], it[1], it[2], it[3], it[4], it[5])}, fasta, fai, "SV" diff --git a/subworkflows/local/vcf_variant_filtering.nf b/subworkflows/local/vcf_variant_filtering.nf index efa98c1..c8d12d6 100644 --- a/subworkflows/local/vcf_variant_filtering.nf +++ b/subworkflows/local/vcf_variant_filtering.nf @@ -55,7 +55,7 @@ workflow VCF_VARIANT_FILTERING { // // filters out smaller SVs than min_sv_size, only applicable to SV files SURVIVOR_FILTER( - vcf.sv.map{it -> tuple( it[0], it[1],[])}, + vcf.sv.map{it -> tuple( it[0], it[1], [])}, params.min_sv_size, params.max_sv_size, params.min_allele_freq, diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 8783597..89517ec 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -172,7 +172,7 @@ workflow VARIANTBENCHMARKING { } else{ test.small.combine(truth.small) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} + .map{it -> tuple(it[0], it[1], it[2], it[4], it[5], [])} .set{bench} bench_ch = bench_ch.mix(bench) } @@ -181,13 +181,13 @@ workflow VARIANTBENCHMARKING { if(params.high_conf_sv){ test.sv.combine(truth.sv) .combine(high_conf.sv) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} + .map{it -> tuple(it[0], it[1], it[2], it[4], it[5], it[7])} .set{bench} bench_ch = bench_ch.mix(bench) } else{ test.sv.combine(truth.sv) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} + .map{it -> tuple(it[0], it[1], it[2], it[4], it[5], [])} .set{bench} bench_ch = bench_ch.mix(bench) } @@ -196,13 +196,13 @@ workflow VARIANTBENCHMARKING { if(params.high_conf_cnv){ test.cnv.combine(truth.cnv) .combine(high_conf.cnv) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],it[7])} + .map{it -> tuple(it[0], it[1], it[2], it[4], it[5], it[7])} .set{bench} bench_ch = bench_ch.mix(bench) } else{ test.cnv.combine(truth.cnv) - .map{it -> tuple(it[0], it[1], it[2],it[4],it[5],[])} + .map{it -> tuple(it[0], it[1], it[2], it[4], it[5], [])} .set{bench} bench_ch = bench_ch.mix(bench) } From 55814542c6f9ab1df7406614acbdce5e51e3aa5d Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 26 Apr 2024 14:05:01 +0000 Subject: [PATCH 055/292] sv benchmark params are exposed --- README.md | 6 +++++ conf/modules.config | 4 +-- conf/test_hg38.config | 21 ++++++++++++--- nextflow.config | 13 ++++++++++ nextflow_schema.json | 60 +++++++++++++++++++++++++++++++++++++++++++ 5 files changed, 98 insertions(+), 6 deletions(-) diff --git a/README.md b/README.md index 682fbac..56b37b2 100644 --- a/README.md +++ b/README.md @@ -47,6 +47,12 @@ Supported SV callers: Manta, SVaba, Dragen, Delly, Lumpy .. Available Truth samples: HG002, SEQC2 +- If you have unresolved SVs, it is recommended to use only truvari with --pctsim 0. + +- The size filtration parameters provided by truvari and svbenchmark do not apply in the same way. That is why using them are not recommended through the pipeline, but in vcf normalization steps, variants can be filtering safely. + +- Please not that it is not possible to use exactly the same parameters for different benchmarking methods. + +The workflow involves several key processes to ensure reliable and reproducible results as follows: - - +### Standardization and normalization of variants: -1. Standardization of SVs in test VCF files -2. Normalization of SVs in test VCF files -3. Normalization of SVs in truth VCF files -4. SV stats and histograms (Survivor) +This initial step ensures consistent formatting and alignment of variants in test and truth VCF files for accurate comparison. -5. Germline benchmarking of small variants - - Tools: - Happy - RTGtools -6. Germline benchmarking of SVs +1. Subsample if input test vcf is multisample ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) +2. Homogenization of multi-allelic variants, MNPs and SVs (including imprecise paired breakends and single breakends) ([variant-extractor](https://github.com/EUCANCan/variant-extractor)) * +3. Reformatting test VCF files from different SV callers ([svync](https://github.com/nvnieuwk/svync)) * +4. Rename sample names in test and truth VCF files ([bcftools reheader](https://samtools.github.io/bcftools/bcftools.html#reheader)) +5. Splitting multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) * +6. Deduplication of variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) * +7. Use prepy in order to normalize test files. This option is only applicable for happy benchmarking of germline analysis ([prepy](https://github.com/Illumina/hap.py/tree/master)) +8. Split SNVs and indels if the given test VCF contains both. This is only applicable for somatic analysis ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) - - Tools: - Truvari - Svbenchmark - Wittyer: Only works with Truth files annotated with SVTYPE and SVLENGHT +### Filtering options: -7. Somatic benchmarking of small variants +Applying filtering on the process of benchmarking itself might makes it impossible to compare different benchmarking strategies. Therefore, for whom like to compare benchmarking methods this subworkflow aims to provide filtering options for variants. - - Tools: - Happy - RTGtools +9. Filtration of contigs ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) +10. Include or exclude SNVs and INDELs ([bcftools filter](https://samtools.github.io/bcftools/bcftools.html#filter)) +11. Size and quality filtering for SVs ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) -8. Final report and comparisons +### Liftover of truth sets: -## Usage +This sub-workflow provides option to convert genome coordinates of truth VCF and high confidence BED file to a new assembly. Golden standard truth files are build upon specific reference genomes which makes the necessity of lifting over depending on the test VCF in query. -> [!NOTE] -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. +12. Create sequence dictionary for the reference ([picard CreateSequenceDictionary](https://gatk.broadinstitute.org/hc/en-us/articles/360037068312-CreateSequenceDictionary-Picard)). This file can be saved and reused. +13. Lifting over truth variants ([picard LiftoverVcf](https://gatk.broadinstitute.org/hc/en-us/articles/360037060932-LiftoverVcf-Picard)) +14. Lifting over high confidence coordinates ([UCSC liftover](http://hgdownload.cse.ucsc.edu/admin/exe)) + +### Statistical inference of input test and truth variants: + +This step provides insights into the distribution of variants before benchmarking. + +15. Get statistics of SNVs, INDELs and complex variants ([bcftools stats](https://samtools.github.io/bcftools/bcftools.html#stats)) +16. Get statistics of SVs by type ([SURVIVOR stats](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) + +### Benchmarking of variants: + +Actual benchmarking of variants are split between SVs and small variants: + +Available methods for SVs: + +17. Germline and somatic variant benchmarking using Truvari ([truvari bench](https://github.com/acenglish/truvari/wiki/bench)) +18. Germline and somatic variant benchmarking using SVanalyzer ([svanalyzer benchmark](https://github.com/nhansen/SVanalyzer/blob/master/docs/svbenchmark.rst)) + +Available methods for CNVs: + +19. Germline and somatic variant benchmarking using Wittyer ([witty.er](https://github.com/Illumina/witty.er/tree/master)) + +Available methods for SNVs and INDELs: + +20. Germline variant benchmarking using RTG-tools ([rtg vcfeval](https://realtimegenomics.com/products/rtg-tools)) +21. Germline variant benchmarking using Happy tools ([hap.py](https://github.com/Illumina/hap.py/blob/master/doc/happy.md)) +22. Somatic variant benchmarking using Sompy ([som.py](https://github.com/Illumina/hap.py/tree/master?tab=readme-ov-file#sompy)) + +### Comparison of benchmarking results per TP, FP and FN files + +It is essential to compare benchmarking results in order to infer uniquely or commonly seen TPs, FPs and FNs. -Supported SV callers: Manta, SVaba, Dragen, Delly, Lumpy .. -Available Truth samples: HG002, SEQC2 +23. Merging TP, FP and FN results for happy, rtgtools and sompy ([bcftools merge](https://samtools.github.io/bcftools/bcftools.html#merge)) +24. Merging TP, FP and FN results for Truvari and SVanalyzer ([SURVIVOR merge](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) +25. Conversion of VCF files to CSV to infer common and unique variants per caller (python script) -- If you have unresolved SVs, it is recommended to use only truvari with --pctsim 0. +### Reporting of benchmark results -- The size filtration parameters provided by truvari and svbenchmark do not apply in the same way. That is why using them is not recommended through the pipeline, but variants can be filtered safely in vcf normalization steps. +The generation of comprehensive report that consolidates all benchmarking results. -- Please note that it is not possible to use exactly the same parameters for different benchmarking methods. +26. Merging summary statistics per benchmarking tool (python script) +27. Plotting benchmark metrics per benchmarking tool (R script) +28. Create visual HTML report for the integration of NCBENCH ([datavzrd](https://datavzrd.github.io/docs/index.html)) + + + + + +## Usage + +> [!NOTE] +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - +Each row represents a vcf file (test-query file). For each vcf file, variant calling method (caller) and variant type (vartype) have to be defined. -Now, you can run the pipeline using: +User has to define or provide truth vcf in config files. There are readily available vcf files for benchmarking from Genome in a bottle and SEQC2 studies which can be used readily. - +For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). + +Now, you can run the pipeline using: ```bash nextflow run nf-core/variantbenchmarking \ -profile \ --input samplesheet.csv \ - --outdir + --outdir \ + --genome GRCh37 \ + --sample HG002 + --analysis germline ``` > [!WARNING] > Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; > see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). -For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). - ## Pipeline output To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page. For more details about the output files and reports, please refer to the [output documentation](https://nf-co.re/variantbenchmarking/output). +This pipeline outputs benchmarking results per method besides to the inferred and compared statistics. + ## Credits -nf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de. +nf-core/variantbenchmarking was originally written by Kübra Narcı ([@kubranarci](https://github.com/kubranarci)) as a part of benchmarking studies in German Human Genome Phenome Archieve Project ([GHGA](https://www.ghga.de/)). We thank the following people for their extensive assistance in the development of this pipeline: - +Nicolas Vannieuwkerke ([@nvnienwk](https://github.com/nvnieuwk)) +Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) + ## Contributions and Support diff --git a/assets/schema_input.json b/assets/schema_input.json index b482cd8..fea32c3 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -20,21 +20,27 @@ "pattern": "^\\S+$", "description": "Sample id", "meta": ["id"], - "minLength": 1 + "minLength": 1, + "errorMessage": "A custom id must be provided per test vcf", + "description": "A custom per test vcf" }, "caller": { "type": "string", "pattern": "^\\S+$", "description": "Name of the variant caller used to generate test file", "meta": ["caller"], - "minLength": 1 + "minLength": 1, + "errorMessage": "Variant caller has to be defined. Can also be unknown, undefined or merged" + }, "vartype": { "type": "string", "pattern": "^\\S+$", - "description": "Variant type to benchmark", + "description": "Variant type to apply benchmarking", "meta": ["vartype"], - "minLength": 1 + "minLength": 1, + "errorMessage": "Variant type can be only one of these: small, sv, snv, indel and cnv" + }, "subsample": { "type": "string", diff --git a/conf/modules.config b/conf/modules.config index 28c5129..1f770f2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -73,10 +73,6 @@ process { withName: BCFTOOLS_VIEW_SUBSAMPLE { ext.prefix = { vcf.baseName - ".vcf" + ".subsample" } ext.args = {"--output-type z -s ${meta.subsample}" } - publishDir = [ - path: { "${params.outdir}/test" }, - enabled: false - ] } withName: BCFTOOLS_VIEW_SNV { ext.prefix = { vcf.baseName - ".vcf" + ".snv" } @@ -352,7 +348,7 @@ process { // Don't publish results for these processes // process { - withName: 'TABIX_TABIX|TABIX_BGZIP|TABIX_BGZIPTABIX|BGZIP_TABIX|SURVIVOR_MERGE|BCFTOOLS_MERGE|REFORMAT_HEADER|BCFTOOLS_NORM|BCFTOOLS_DEDUP|BCFTOOLS_REHEADER|SORT_BED|UCSC_LIFTOVER|PICARD_LIFTOVERVCF' { + withName: 'TABIX_TABIX|TABIX_BGZIP|TABIX_BGZIPTABIX|BGZIP_TABIX|SURVIVOR_MERGE|BCFTOOLS_MERGE|REFORMAT_HEADER|BCFTOOLS_NORM|BCFTOOLS_DEDUP|BCFTOOLS_REHEADER|SORT_BED|UCSC_LIFTOVER|PICARD_LIFTOVERVCF|BCFTOOLS_VIEW_SUBSAMPLE' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false diff --git a/docs/output.md b/docs/output.md index c671e3f..3bce603 100644 --- a/docs/output.md +++ b/docs/output.md @@ -2,7 +2,7 @@ ## Introduction -This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarises results at the end of the pipeline. +This document describes the output produced by the pipeline. Most of the plots are taken from the MultiQC report, which summarizes results at the end of the pipeline. The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. @@ -12,32 +12,151 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC +- [Preprocesses ](#preprocesses) +- [Liftover of truth sets](#liftover) +- [Input vcf statistics](#stats) +- [Benchmarking](#bench) + - [Truvari](#truvari_bench) + - [SVanalyzer](#svanalyzer_bench) + - [Wittyer](#wittyer_bench) + - [RTG-tools](#rtgtools_bench) + - [Happy](#happy_bench) + - [Sompy](#sompy_bench) +- [Summary statistics](#summary) + - [Comparison of benchmarking results](#comparisons) + - [Merged summary benchmark statistics](#tables) + - [Plots](#plots) + - [datavzrd HTML reports](#html) - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution -### FastQC +### Preprocesses
    Output files -- `fastqc/` - - `*_fastqc.html`: FastQC report containing quality metrics. - - `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images. +- `preprocesses/` + - `*vcf.gz`: The standardized and normalized VCF files
    -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). +Outputs from standardization, normalization and filtration processes saved. When any of `--sv_standardization`, `--preprocesses` or filtration applied to the input set of variants, the processed outputs will be saved into this directory. -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) +For test VCFs structuring as follows: -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) +*id*/*preprocess*/ -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) +For truth VCFs structuring as follows: -:::note -The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -::: +*sample*/*preprocess*/ + +### Liftover of truth sets + +
    +Output files + +- `liftover/` + - + +
    + +If liftover applied ... + +### Input VCF statistics + +
    +Output files + +- `stats/` + - `bcftools/` + - '*.bcftools_stats.txt' + - `survivor/` + - '*.stats' + +
    + +bcftools stats applied into all variant types while survivor stats is only available for structural variants. + + +### Benchmarking + +
    +Output files + +- `truvari_bench/` + - `*.fn.vcf.gz` + - `*.fn.vcf.gz.tbi` + - `*.fp.vcf.gz` + - `*.fp.vcf.gz.tbi` + - `*.tp-comp.vcf.gz` + - `*.tp-comp.vcf.gz.tbi` + - `*.tp-base.vcf.gz` + - `*.tp-base.vcf.gz.tbi` + - `*.summary.json` +- `svanalyzer_bench/` + - `*.distances` + - `*.falsenegatives.vcf.gz` + - `*.falsepositives.vcf.gz` + - `*.log` + - `*.report` +- `wittyer_bench/` + - `*.vcf.gz` + - `*.vcf.gz.tbi` + - `*.json` +- `rtgtools_bench/` + - `*.vcf.gz` + - `*.vcf.gz.tbi` + - `*.fn.vcf.gz` + - `*.fn.vcf.gz.tbi` + - `*.fp.vcf.gz` + - `*.fp.vcf.gz.tbi` + - `*.tp.vcf.gz` + - `*.tp.vcf.gz.tbi` + - `*.tp-baseline.vcf.gz` + - `*.tp-baseline.vcf.gz.tbi` + - `*.non_snp_roc.tsv.gz` + - `*.phasing.txt` + - `*.snp_roc.tsv.gz` + - `*.summary.txt` + - `*.weighted_roc.tsv.gz` +- `happy_bench/` + - `*.extended.csv` + - `*.metrics.json.gz` + - `*.roc.all.csv.gz` + - `*.roc.Locations.INDEL.csv.gz` + - `*roc.Locations.INDEL.PASS.csv.gz` + - `*roc.Locations.SNP.csv.gz` + - `*roc.Locations.SNP.PASS.csv.gz` + - `*.runinfo.json` + - `*.summary.csv` + - `*.vcf.gz` + - `*.vcf.gz.tbi` +- `sompy_bench/` + - + +
    + +Benchmark results are created separately for each test vcf: + +*id*/*_bench_*/ + + +### Summary statistics + +
    +Output files + +- `comparisons/` + - +- `plots/` + - +- `tables/` + - +- `html/` + - + +
    +...... ### MultiQC diff --git a/docs/usage.md b/docs/usage.md index 63d5e6b..09406b8 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -10,54 +10,123 @@ ## Samplesheet input -You will need to create a samplesheet with information about the samples you would like to analyse before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. +You will need to create a samplesheet with information about the test vcf you would like to analyze before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row as shown in the examples below. ```bash --input '[path to samplesheet file]' ``` -### Multiple runs of the same sample +### Full samplesheet -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: +The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below. ```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz -CONTROL_REP1,AEG588A1_S1_L004_R1_001.fastq.gz,AEG588A1_S1_L004_R2_001.fastq.gz +id,test_vcf,caller,vartype +test1,test1.vcf.gz,delly,sv +test2,test2.vcf,gatk,small +test3,test3.vcf.gz,cnvkit,cnv ``` -### Full samplesheet +| Column | Description | +| ---------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | +| `id` | Custom id name per test vcf. This entry will be identical. | +| `test_vcf` | The VCF file to use as benchmarking test input. The same file can be used in more than one row. File can be either vcf or vcf.gz. | +| `caller` | Variant caller method used to generate test VCF file. There can be more than one test vcf for the same caller. For unknown caller use 'unknown' | +| `vartype` | Variant type to apply benchmarking. Variant type can be only one of these: small, sv, snv, indel and cnv. | + +An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. + + +## Defining Truth VCF and High confidence BED files + +The following parameters has to be defined for each type of benchmarking analysis. The following parameters defined the exact paths to the truth files: + +- `--sample`: Sample parameter defines the same name of the truth set. Examples: `HG002`, `SEQC2`, `HG001`, `HG003`, `CHM13`. +- `--analysis`: The type of analysis to perform: `germline` or `somatic`. +- `--method`: List the benchmarking methods to apply. By default all available tools will be applied according to the variant types provided. Available tools: `truvari, svanalyzer, happy, sompy, rtgtools, wittyer`. + +*Small variant benchmarking:* + +- `--truth_small`: Path to the golden set VCF files combined for SNVs and indels, required for germline benchmarking (vcf or vcf.gz) +- `--high_conf_small`: Path to the high confidence BED files for SNVs and indels, required for germline benchmarking (bed or bed.gz) +- `--truth_snv`: Path to the golden set VCF files for SNVs, required for somatic benchmarking (vcf or vcf.gz) +- `--high_conf_snv`: Path to the high confidence BED files for SNVs, required for somatic benchmarking (bed or bed.gz) +- `--truth_indel`: Path to the golden set VCF files for indels, required for somatic benchmarking (vcf or vcf.gz) +- `--high_conf_indel`: Path to the high confidence BED files for indels, required for somatic benchmarking (bed or bed.gz) + +*Structural variant benchmarking:* + +- `--truth_sv`: Path to the golden set VCF files for SVs, required for germline and somatic benchmarking (vcf or vcf.gz) +- `--high_conf_sv`: Path to the high confidence BED files for SVs, required for germline and somatic benchmarking (bed or bed.gz) + +*Copy Number Variation benchmarking:* + +- `--truth_cnv`: Path to the golden set VCF files for CNVs, required for germline and somatic benchmarking (vcf or vcf.gz) +- `--high_conf_cnv`: Path to the high confidence BED files for CNVs, required for germline and somatic benchmarking (bed or bed.gz) + +*Using truth.config* + +`conf/truth.config` file contains some readily available truth files for germline and somatic analysis. In order to activate usage one has to + +1. use `--genome` [`GRCh37` or `GRCh38`] +2. define `--sample` [`HG002` or `SEQC2`] +3. turn off `--itruth_ignore false` -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. +## Lifting over truth sets + +This workflow comes with a liftover option for truth sets. In order to activate liftover use `--liftover true`. + +- `--chain`: This workflow uses picard tools for lifting over and a chain file has to be provided specific to the input truth vcf. Some examples can be found [here](https://genome.ucsc.edu/goldenPath/help/chain.html) +- `--rename_chr`: Renaming chromosomes is required after liftover process. Some examples can be found under `assets/rename_contigs` directory. + +Note: these two files are also provided under `itruth.config`. An example usage can be found in `conf/test_liftover.config` + +## Standardization and normalization parameters + +Consistent formatting and alignment of variants in test and truth VCF files for accurate comparison is controlled by *sv_standardization* and *preprocesses*. + +- `--sv_standardization`: The standardization methods to perform on the input files. Should be a comma-separated list of one or more of the following options: `homogenize,svync`. + - `homogenize`: makes use of [variant-extractor](https://github.com/EUCANCan/variant-extractor) + - `svync`: makes use of [svync](https://github.com/nvnieuwk/svync) + +- `--preprocesses`: The preprocessing steps to perform on the input files. Should be a comma-separated list of one or more of the following options: `normalization,deduplication,prepy,filter_contigs` + - `normalization`: Splits multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) + - `deduplication`: Deduplicates variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) + - `prepy`: Uses prepy in order to normalize test files. This option is only applicable for happy benchmarking of germline analysis ([prepy](https://github.com/Illumina/hap.py/tree/master)) + - `filter_contigs`: Filter out extra contigs. It is common for truth files not to include extra contigs. + +## Using multi-sample vcf inputs + +If the input test vcf contains more than one sample, then user has to define which sample name to use. `subsample` will added to the samplesheet as an additional column as follows: -A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. ```csv title="samplesheet.csv" -sample,fastq_1,fastq_2 -CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz -CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz -CONTROL_REP3,AEG588A3_S3_L002_R1_001.fastq.gz,AEG588A3_S3_L002_R2_001.fastq.gz -TREATMENT_REP1,AEG588A4_S4_L003_R1_001.fastq.gz, -TREATMENT_REP2,AEG588A5_S5_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L003_R1_001.fastq.gz, -TREATMENT_REP3,AEG588A6_S6_L004_R1_001.fastq.gz, +id,test_vcf,caller,vartype,subsample +test1,test1.vcf.gz,delly,sv,"TUMOR" +test2,test2.vcf,gatk,small,"NA128120" +test3,test3.vcf.gz,cnvkit,cnv, ``` -| Column | Description | -| --------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +Note that, this option can be inevitable for somatic analysis since most of the callers reports both normal and tumor genotypes in the same vcf file. -An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. +## Filtering parameters + +- `--exclude_expression`: Use [bcftools expressions](https://samtools.github.io/bcftools/bcftools.html#expressions) to exclude variants. Default:null +- `--include_expression`: Use [bcftools expressions](https://samtools.github.io/bcftools/bcftools.html#expressions) to include variants. Default:null + +*Parameters applicable only to Structural Variants* + +- `--min_sv_size`: Minimum SV size of variants to benchmark, 0 to disable , Default:30 +- `--max_sv_size`: Maximum SV size of variants to benchmark, -1 to disable , Default:-1 +- `--min_allele_freq`: Minimum Alele Frequency of variants to benchmark, Use -1 to disable , Default:-1 +- `--min_num_reads`: Minimum number of read supporting variants to benchmark, Use, -1 to disable , Default:-1 ## Running the pipeline The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/variantbenchmarking --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker +nextflow run nf-core/variantbenchmarking --input ./samplesheet.csv --outdir ./results -profile docker --genome GRCh37 --sample HG002 --analysis germline ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -91,6 +160,8 @@ with `params.yaml` containing: input: './samplesheet.csv' outdir: './results/' genome: 'GRCh37' +sample: 'HG002' +analysis: 'germline' <...> ``` @@ -144,6 +215,15 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - `test` - A profile with a complete configuration for automated testing - Includes links to test data so needs no other parameters +- `test_liftover` + - A profile with a complete configuration for using liftover of HG002 hg38 truth set to hg37 + - Includes links to test data so needs no other parameters +- `test_germline` + - A profile with a complete configuration for a full test of HG002 sample from germline analysis + - Includes links to test data so needs no other parameters +- `test_somatic` + - A profile with a complete configuration for a full test of SEQC2 sample from somatic analysis + - Includes links to test data so needs no other parameters - `docker` - A generic configuration profile to be used with [Docker](https://docker.com/) - `singularity` diff --git a/nextflow.config b/nextflow.config index ea557a7..3246392 100644 --- a/nextflow.config +++ b/nextflow.config @@ -31,7 +31,7 @@ params { // minsize effects both truvari and variantbenchmarkingmark in different ways! svbechmark filters both base and comp calls // although with truvari it is possible to filter only base, which makes more sense to me! // I will use this parameter to filter SVs before benchmarking! - min_sv_size = 50 + min_sv_size = 30 max_sv_size = -1 min_allele_freq = -1 min_num_reads = -1 diff --git a/nextflow_schema.json b/nextflow_schema.json index 3023571..f4fd985 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -31,7 +31,21 @@ }, "sample": { "type": "string", - "description": "Sample id: HG002/SEQC2", + "description": "Sample id", + "fa_icon": "fas fa-folder-open", + "description": "The sample name of the truth case. Examples: HG002, SEQC2, HG001, HG003, CHM13" + }, + "analysis": { + "type": "string", + "description": "The analysis type used by the input files", + "enum": ["germline", "somatic"], + "fa_icon": "fas fa-folder-open" + }, + "method": { + "type": "string", + "description": "The benchmarking methods to use. Should be a comma-separate list of one or more of the following options: truvari, svanalyzer, happy, sompy, rtgtools, wittyer", + "errorMessage": "A wrong input has been detected. Should be a comma-separated list of one or more of the following options: truvari, svanalyzer, happy, sompy, rtgtools, wittyer", + "pattern": "^((truvari|svanalyzer|happy|sompy|rtgtools|wittyer)?,?)*(? Date: Fri, 9 Aug 2024 14:04:27 +0200 Subject: [PATCH 162/292] a small fix and cont of documentation --- assets/schema_input.json | 4 +- conf/modules.config | 6 +- docs/output.md | 162 +++++++++++++++-------- nextflow_schema.json | 12 +- subworkflows/local/prepare_vcfs_truth.nf | 5 +- workflows/variantbenchmarking.nf | 2 +- 6 files changed, 123 insertions(+), 68 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index fea32c3..8eda41b 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -21,8 +21,7 @@ "description": "Sample id", "meta": ["id"], "minLength": 1, - "errorMessage": "A custom id must be provided per test vcf", - "description": "A custom per test vcf" + "errorMessage": "A custom id must be provided per test vcf" }, "caller": { "type": "string", @@ -40,7 +39,6 @@ "meta": ["vartype"], "minLength": 1, "errorMessage": "Variant type can be only one of these: small, sv, snv, indel and cnv" - }, "subsample": { "type": "string", diff --git a/conf/modules.config b/conf/modules.config index 1f770f2..7fb41ed 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -265,7 +265,7 @@ process { withName: MERGE_REPORTS { ext.prefix = {"${meta.benchmark_tool}.${meta.vartype}"} publishDir = [ - path: {"${params.outdir}/summary/tables"}, + path: {"${params.outdir}/summary/tables/${meta.vartype}/${meta.benchmark_tool}"}, pattern: "*{csv}", mode: params.publish_dir_mode ] @@ -273,7 +273,7 @@ process { withName: PLOTS { ext.prefix = {"${meta.benchmark_tool}.${meta.vartype}"} publishDir = [ - path: {"${params.outdir}/summary/plots"}, + path: {"${params.outdir}/summary/plots/${meta.vartype}/${meta.benchmark_tool}"}, pattern: "*{png}", mode: params.publish_dir_mode ] @@ -292,7 +292,7 @@ process { withName: VCF_TO_CSV { ext.prefix = {"${meta.id}.${meta.vartype}.${meta.tag}"} publishDir = [ - path: {"${params.outdir}/summary/comparisons"}, + path: {"${params.outdir}/summary/comparisons/${meta.vartype}"}, pattern: "*{.csv}", mode: params.publish_dir_mode ] diff --git a/docs/output.md b/docs/output.md index 3bce603..577bcd7 100644 --- a/docs/output.md +++ b/docs/output.md @@ -6,8 +6,6 @@ This document describes the output produced by the pipeline. Most of the plots a The directories listed below will be created in the results directory after the pipeline has finished. All paths are relative to the top-level results directory. - - ## Pipeline overview The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: @@ -48,7 +46,7 @@ For test VCFs structuring as follows: For truth VCFs structuring as follows: -*sample*/*preprocess*/ +sample/preprocess/ ### Liftover of truth sets @@ -84,61 +82,63 @@ bcftools stats applied into all variant types while survivor stats is only avail Output files - `truvari_bench/` - - `*.fn.vcf.gz` - - `*.fn.vcf.gz.tbi` - - `*.fp.vcf.gz` - - `*.fp.vcf.gz.tbi` - - `*.tp-comp.vcf.gz` - - `*.tp-comp.vcf.gz.tbi` - - `*.tp-base.vcf.gz` - - `*.tp-base.vcf.gz.tbi` - - `*.summary.json` + - `*.fn.vcf.gz` : False negative calls from comparison + - `*.fn.vcf.gz.tbi` : False negative calls from comparison - index file + - `*.fp.vcf.gz`: False positive calls from comparison + - `*.fp.vcf.gz.tbi`: False positive calls from comparison - index file + - `*.tp-comp.vcf.gz`: True positive calls from the comparison VCF + - `*.tp-comp.vcf.gz.tbi`: True positive calls from the comparison VCF - index file + - `*.tp-base.vcf.gz`: True positive calls form the base VCF + - `*.tp-base.vcf.gz.tbi`: True positive calls form the base VCF - index file + - `*.summary.json`: Json output of performance stats - `svanalyzer_bench/` - - `*.distances` - - `*.falsenegatives.vcf.gz` - - `*.falsepositives.vcf.gz` - - `*.log` - - `*.report` + - `*.distances`: Distances for comparisons + - `*.falsenegatives.vcf.gz` : False negative calls from comparison + - `*.falsepositives.vcf.gz`: False positive calls from comparison + - `*.log`: Log of the run + - `*.report`: Output report of performance stats - `wittyer_bench/` - - `*.vcf.gz` - - `*.vcf.gz.tbi` - - `*.json` + - `*.vcf.gz`: Calls from comparison + - `*.vcf.gz.tbi`: Calls from comparison - index file + - `*.json`: Json output of performance stats - `rtgtools_bench/` - - `*.vcf.gz` - - `*.vcf.gz.tbi` - - `*.fn.vcf.gz` - - `*.fn.vcf.gz.tbi` - - `*.fp.vcf.gz` - - `*.fp.vcf.gz.tbi` - - `*.tp.vcf.gz` - - `*.tp.vcf.gz.tbi` - - `*.tp-baseline.vcf.gz` - - `*.tp-baseline.vcf.gz.tbi` - - `*.non_snp_roc.tsv.gz` - - `*.phasing.txt` - - `*.snp_roc.tsv.gz` - - `*.summary.txt` - - `*.weighted_roc.tsv.gz` + - `*.vcf.gz`: Calls from comparison + - `*.vcf.gz.tbi`: Calls from comparison - index file + - `*.fn.vcf.gz` : Contains variants from the baseline VCF which were not correctly called + - `*.fn.vcf.gz.tbi` : Contains variants from the baseline VCF which were not correctly called - index file + - `*.fp.vcf.gz`: Contains variants from the calls VCF which do not agree with baseline variants + - `*.fp.vcf.gz.tbi`: Contains variants from the calls VCF which do not agree with baseline variants - index file + - `*.tp.vcf.gz`: Contains those variants from the calls VCF which agree with variants in the baseline VCF + - `*.tp.vcf.gz.tbi`: Contains those variants from the calls VCF which agree with variants in the baseline VCF - index file + - `*.tp-baseline.vcf.gz`: Contains those variants from the baseline VCF which agree with variants in the calls VCF + - `*.tp-baseline.vcf.gz.tbi`: Contains those variants from the baseline VCF which agree with variants in the calls VCF - index file + - `*.non_snp_roc.tsv.gz`: Contains ROC data derived from those variants which were not represented as SNPs + - `*.phasing.txt`: Contains phasing information + - `*.snp_roc.tsv.gz`: Contains ROC data derived from only those variants which were represented as SNPs + - `*.summary.txt`: Output summary of performance stats + - `*.weighted_roc.tsv.gz`: Contains ROC data derived from all analyzed call variants, regardless of their representation - `happy_bench/` - - `*.extended.csv` - - `*.metrics.json.gz` - - `*.roc.all.csv.gz` - - `*.roc.Locations.INDEL.csv.gz` - - `*roc.Locations.INDEL.PASS.csv.gz` - - `*roc.Locations.SNP.csv.gz` - - `*roc.Locations.SNP.PASS.csv.gz` - - `*.runinfo.json` - - `*.summary.csv` - - `*.vcf.gz` - - `*.vcf.gz.tbi` + - `*.extended.csv`: Extended statistics + - `*.metrics.json.gz`: JSON file containing all computed metrics and tables + - `*.roc.all.csv.gz`: All precision / recall data points that were calculated + - `*.roc.Locations.INDEL.csv.gz`: ROC for ALL indels only. + - `*roc.Locations.INDEL.PASS.csv.gz`: ROC for PASSing indels only. + - `*roc.Locations.SNP.csv.gz`: ROC for ALL SNPs only. + - `*roc.Locations.SNP.PASS.csv.gz`: ROC for PASSing SNPs only. + - `*.runinfo.json`: Log of the run + - `*.summary.csv`: Output summary of performance stats + - `*.vcf.gz`: Calls from comparison + - `*.vcf.gz.tbi`: Calls from comparison - index file - `sompy_bench/` - - + - `*.features.csv`: Calls from comparison + - `*.metrics.json`: JSON file containing all computed metrics and tables + - `*.stats.csv`: Output summary of performance stats Benchmark results are created separately for each test vcf: -*id*/*_bench_*/ +id/_bench_/ ### Summary statistics @@ -147,16 +147,74 @@ Benchmark results are created separately for each test vcf: Output files - `comparisons/` - - + - `small/` + - `rtgtools.small.FN.csv`: Summarizes and compares variants from the baseline VCF of rtgtools which were not correctly called + - `rtgtools.small.FP.csv`: Summarizes and compares variants from the calls VCF of rtgtools which do not agree with baseline variant + - `rtgtools.small.TP_base.csv`: Summarizes and compares variants from the baseline VCF of rtgtools which were correctly called + - `rtgtools.small.TP_comp.csv`: Summarizes and compares variants from the calls VCF of rtgtools which do agree with baseline variant + - `sv/` + - `svbenchmark.sv.FN.csv`: Summarizes and compares variants from the baseline VCF of svbenchmark which were not correctly called + - `svbenchmark.sv.FP.csv`: Summarizes and compares variants from the calls VCF of svbenchmark which do not agree with baseline variant + - `truvari.sv.FN.csv`: Summarizes and compares variants from the baseline VCF of truvari which were not correctly called + - `truvari.sv.FP.csv`: Summarizes and compares variants from the calls VCF of truvari which do not agree with baseline variant + - `truvari.sv.TP_base.csv`: Summarizes and compares variants from the baseline VCF of truvari which were correctly called + - `truvari.sv.TP_comp.csv`: Summarizes and compares variants from the calls VCF of truvari which do agree with baseline variant - `plots/` - - + - `cnv/` + - `wittyer/` + - `Base_metric_by_tool_wittyer.png`: Summary plot for callers on precision, recall and F1 per base in wittyer + - `Base_variants_by_tool_wittyer.png`: Summary plot for callers on TP, FP and FN numbers per base in wittyer + - `Event_metric_by_tool_wittyer.png`: Summary plot for callers on precision, recall and F1 per event in wittyer + - `Event_variants_by_tool_wittyer.png`: Summary plot for callers on TP, FP and FN numbers per ecent in wittyer + - `sv/` + - `truvari/` + - `metric_by_tool_truvari.png`: Summary plot for callers on precision, recall and F1 in truvari + - `variants_by_tool_truvari.png`: Summary plot for callers on TP, FP and FN numbers in truvari + - `svbenchmark/` + - `metric_by_tool_svbenchmark.png`: Summary plot for callers on precision, recall and F1 in svbenchmark + - `variants_by_tool_svbenchmark.png`: Summary plot for callers on TP, FP and FN numbers in svbenchmark + - `small/` + - `happy/` + - `INDEL_ALL_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of all INDELs in happy + - `INDEL_ALL_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of all INDELs in happy + - `INDEL_PASS_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of only PASSed INDELs in happy + - `INDEL_PASS_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of only PASSed INDELs in happy + - `SNP_ALL_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of all SNPs in happy + - `SNP_ALL_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of all SNPs in happy + - `SNP_PASS_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of only PASSed SNPs in happy + - `SNP_PASS_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of only PASSed SNPs in happy + - `rtgtools/` + - `metric_by_tool_rtgtools.png`: Summary plot for callers on precision, recall and F1 in rtgtools + - `variants_by_tool_rtgtools.png`: Summary plot for callers on TP, FP and FN numbers in rtgtools + - `indel/` + - `sompy/` + - `metric_by_tool_sompy.png`: Summary plot for callers on precision, recall and F1 of indels in sompy + - `variants_by_tool_sompy.png`: Summary plot for callers on TP, FP and FN numbers of indels in sompy + - `snv/` + - `sompy/` + - `metric_by_tool_sompy.png`: Summary plot for callers on precision, recall and F1 of SNVs in sompy + - `variants_by_tool_sompy.png`: Summary plot for callers on TP, FP and FN numbers of SNVs in sompy - `tables/` - - + - `cnv/` + - `wittyer.cnv.summary.csv`: Summary of performance stats from callers + - `sv/` + - `truvari.sv.summary.csv`: Summary of performance stats from callers + - `svbenchmark.sv.summary.csv`: Summary of performance stats from callers + - `small/` + - `happy.sv.summary.csv`: Summary of performance stats from callers + - `rtgtools.sv.summary.csv`: Summary of performance stats from callers + - `indel/` + - `sompy.indel.summary.csv`: Summary of performance stats from callers + - `sompy.indel.regions.csv`: Summary of performance stats split by region bins from callers + - `snv/` + - `sompy.snv.summary.csv`: Summary of performance stats from callers + - `sompy.snv.regions.csv`: Summary of performance stats split by region bins from callers - `html/` - -...... + +Note that comparison results for happy and wittyer is missing since their output does not have FP/TP/FN called variants separably. For svbenchmark, TP_base and TP_comp are also missing from the same reason. ### MultiQC diff --git a/nextflow_schema.json b/nextflow_schema.json index f4fd985..4f1518d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -33,7 +33,7 @@ "type": "string", "description": "Sample id", "fa_icon": "fas fa-folder-open", - "description": "The sample name of the truth case. Examples: HG002, SEQC2, HG001, HG003, CHM13" + "errorMessage": "The sample name of the truth case. Examples: HG002, SEQC2, HG001, HG003, CHM13" }, "analysis": { "type": "string", @@ -53,7 +53,7 @@ "format": "file-path", "exists": true, "pattern": "^\\S+\\.(bed|vcf)?(\\.gz)?$", - "description": "SMALL: Path to the high confidence BED files.", + "description": "SMALL: Path to the high confidence BED or VCF files.", "help_text": "SMALL: High confidence BED files", "fa_icon": "fas fa-file-csv" }, @@ -71,7 +71,7 @@ "format": "file-path", "exists": true, "pattern": "^\\S+\\.(bed|vcf)?(\\.gz)?$", - "description": "SNVs: Path to the high confidence BED files.", + "description": "SNVs: Path to the high confidence BED or VCF files.", "help_text": "SNVs: High confidence BED files", "fa_icon": "fas fa-file-csv" }, @@ -89,7 +89,7 @@ "format": "file-path", "exists": true, "pattern": "^\\S+\\.(bed|vcf)?(\\.gz)?$", - "description": "INDELs: Path to the high confidence BED files.", + "description": "INDELs: Path to the high confidence BED or VCF files.", "help_text": "INDELs: High confidence BED files", "fa_icon": "fas fa-file-csv" }, @@ -107,7 +107,7 @@ "format": "file-path", "exists": true, "pattern": "^\\S+\\.(bed|vcf)?(\\.gz)?$", - "description": "SV: Path to the high confidence BED files.", + "description": "SV: Path to the high confidence BED or VCF files.", "help_text": "SV: High confidence BED files", "fa_icon": "fas fa-file-csv" }, @@ -125,7 +125,7 @@ "format": "file-path", "exists": true, "pattern": "^\\S+\\.(bed|vcf)?(\\.gz)?$", - "description": "CNV: Path to the high confidence BED files.", + "description": "CNV: Path to the high confidence BED or VCF files.", "help_text": "CNV: High confidence BED files", "fa_icon": "fas fa-file-csv" }, diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index f2783f6..8f21443 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -22,7 +22,6 @@ workflow PREPARE_VCFS_TRUTH { main: versions = Channel.empty() - bed_high_conf = Channel.empty() // if liftover option is set convert truth files if (params.liftover){ @@ -36,7 +35,7 @@ workflow PREPARE_VCFS_TRUTH { ) versions = versions.mix(LIFTOVER_VCFS_TRUTH.out.versions.first()) truth_ch = LIFTOVER_VCFS_TRUTH.out.vcf_ch - bed_high_conf = LIFTOVER_VCFS_TRUTH.out.bed_ch + high_conf_ch = LIFTOVER_VCFS_TRUTH.out.bed_ch } // Reheader sample name for truth file - using meta.caller @@ -78,6 +77,6 @@ workflow PREPARE_VCFS_TRUTH { emit: vcf_ch - bed_high_conf + high_conf_ch versions } diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index e0cfa30..e768c94 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -185,7 +185,7 @@ workflow VARIANTBENCHMARKING { chain, rename_chr ) - high_conf_ch = PREPARE_VCFS_TRUTH.out.bed_high_conf + high_conf_ch = PREPARE_VCFS_TRUTH.out.high_conf_ch ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) // VCF REPORTS AND STATS From 3aba3ca77f00bf46735216b95760126588cffd03 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 12 Aug 2024 10:14:12 +0200 Subject: [PATCH 163/292] Update assets/schema_input.json Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- assets/schema_input.json | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index 8eda41b..fe35add 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -38,7 +38,8 @@ "description": "Variant type to apply benchmarking", "meta": ["vartype"], "minLength": 1, - "errorMessage": "Variant type can be only one of these: small, sv, snv, indel and cnv" + "errorMessage": "Variant type can be only one of these: small, sv, snv, indel and cnv", + "enum": ["small", "sv", "snv", "indel", "cnv"] }, "subsample": { "type": "string", From 5b280d26c941f8891479aeb27952b645f26ee197 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 12 Aug 2024 11:19:50 +0000 Subject: [PATCH 164/292] add output info --- README.md | 12 ++-- assets/schema_input.json | 1 - conf/modules.config | 9 ++- docs/output.md | 45 ++++++++------ docs/usage.md | 76 ++++++++++++++++++----- nextflow_schema.json | 13 +++- subworkflows/local/liftover_vcfs_truth.nf | 14 +++-- subworkflows/local/prepare_vcfs_truth.nf | 4 +- workflows/variantbenchmarking.nf | 9 ++- 9 files changed, 124 insertions(+), 59 deletions(-) diff --git a/README.md b/README.md index a238b55..500ca16 100644 --- a/README.md +++ b/README.md @@ -28,11 +28,11 @@ The workflow involves several key processes to ensure reliable and reproducible This initial step ensures consistent formatting and alignment of variants in test and truth VCF files for accurate comparison. 1. Subsample if input test vcf is multisample ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) -2. Homogenization of multi-allelic variants, MNPs and SVs (including imprecise paired breakends and single breakends) ([variant-extractor](https://github.com/EUCANCan/variant-extractor)) * -3. Reformatting test VCF files from different SV callers ([svync](https://github.com/nvnieuwk/svync)) * +2. Homogenization of multi-allelic variants, MNPs and SVs (including imprecise paired breakends and single breakends) ([variant-extractor](https://github.com/EUCANCan/variant-extractor)) +3. Reformatting test VCF files from different SV callers ([svync](https://github.com/nvnieuwk/svync)) 4. Rename sample names in test and truth VCF files ([bcftools reheader](https://samtools.github.io/bcftools/bcftools.html#reheader)) -5. Splitting multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) * -6. Deduplication of variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) * +5. Splitting multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) +6. Deduplication of variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) 7. Use prepy in order to normalize test files. This option is only applicable for happy benchmarking of germline analysis ([prepy](https://github.com/Illumina/hap.py/tree/master)) 8. Split SNVs and indels if the given test VCF contains both. This is only applicable for somatic analysis ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) @@ -94,17 +94,14 @@ The generation of comprehensive report that consolidates all benchmarking result 27. Plotting benchmark metrics per benchmarking tool (R script) 28. Create visual HTML report for the integration of NCBENCH ([datavzrd](https://datavzrd.github.io/docs/index.html)) - - ## Usage > [!NOTE] > If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - First, prepare a samplesheet with your input data that looks as follows: `samplesheet.csv`: @@ -155,7 +152,6 @@ We thank the following people for their extensive assistance in the development Nicolas Vannieuwkerke ([@nvnienwk](https://github.com/nvnieuwk)) Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) - ## Contributions and Support If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). diff --git a/assets/schema_input.json b/assets/schema_input.json index fe35add..fb67fd7 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -30,7 +30,6 @@ "meta": ["caller"], "minLength": 1, "errorMessage": "Variant caller has to be defined. Can also be unknown, undefined or merged" - }, "vartype": { "type": "string", diff --git a/conf/modules.config b/conf/modules.config index 7fb41ed..ae2799b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -114,7 +114,7 @@ process { mode: params.publish_dir_mode ] } - withName: BCFTOOLS_VIEW_FILTERMISSING { + withName: BCFTOOLS_VIEW_FILTERMISSING { ext.prefix = { vcf.baseName - ".vcf" + ".filtermiss" } ext.args = {"--output-type z -e 'AC=0'" } publishDir = [ @@ -133,7 +133,7 @@ process { enabled: false ] } - // stats + // Variant stats withName: SURVIVOR_STATS { ext.prefix ={["${meta.id}.${meta.vartype}", (meta.caller != null) ? ".${meta.caller}" : '' @@ -160,7 +160,7 @@ process { withName: "RTGTOOLS_FORMAT" { ext.when = { params.method.split(',').contains('rtgtools') && !params.sdf } publishDir = [ - path: {"${params.outdir}/rtgtools/sdf"}, + path: {"${params.outdir}/references/rtgtools"}, pattern: "*{.sdf}", mode: params.publish_dir_mode ] @@ -193,7 +193,6 @@ process { mode: params.publish_dir_mode ] } - withName: "HAPPY_PREPY" { ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} ext.args = {"--fixchr --filter-nonref --bcftools-norm"} @@ -307,7 +306,7 @@ process { withName: PICARD_CREATESEQUENCEDICTIONARY { ext.when = { params.liftover } publishDir = [ - path: {"${params.outdir}/${params.sample}/liftover"}, + path: {"${params.outdir}/references/dictionary"}, pattern: "*{.dict}", mode: params.publish_dir_mode ] diff --git a/docs/output.md b/docs/output.md index 577bcd7..d36fd14 100644 --- a/docs/output.md +++ b/docs/output.md @@ -34,31 +34,26 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d Output files - `preprocesses/` - - `*vcf.gz`: The standardized and normalized VCF files + - `*.vcf.gz`: The standardized and normalized VCF files Outputs from standardization, normalization and filtration processes saved. When any of `--sv_standardization`, `--preprocesses` or filtration applied to the input set of variants, the processed outputs will be saved into this directory. -For test VCFs structuring as follows: - -*id*/*preprocess*/ - -For truth VCFs structuring as follows: - -sample/preprocess/ - ### Liftover of truth sets
    Output files +- ## `liftover/` + - `liftover/` - - + - `*.vcf.gz`: Lifted over variants + - `*.bed`: Lifted over regions
    -If liftover applied ... +If liftover applied to the truth set, the lifted over golden set variants (vcf.gz) and high confidence bed file saved here. ### Input VCF statistics @@ -67,15 +62,14 @@ If liftover applied ... - `stats/` - `bcftools/` - - '*.bcftools_stats.txt' + - '\*.bcftools_stats.txt' - `survivor/` - - '*.stats' + - '\*.stats' bcftools stats applied into all variant types while survivor stats is only available for structural variants. - ### Benchmarking
    @@ -136,10 +130,7 @@ bcftools stats applied into all variant types while survivor stats is only avail
    -Benchmark results are created separately for each test vcf: - -id/_bench_/ - +Benchmark results are created separately for each test vcf and for each method used. ### Summary statistics @@ -209,13 +200,27 @@ id/_bench_/ - `snv/` - `sompy.snv.summary.csv`: Summary of performance stats from callers - `sompy.snv.regions.csv`: Summary of performance stats split by region bins from callers -- `html/` - - +- ## `html/` Note that comparison results for happy and wittyer is missing since their output does not have FP/TP/FN called variants separably. For svbenchmark, TP_base and TP_comp are also missing from the same reason. +### References + +
    +Output files + +- `references/` + - `dictionary` + - `*.dict`: Dictionary file is the output of PICARD CREATESEQUENCEDICTIONARY. This file can be saved and reused further. + - `sdf` + - `*.sdf`: Sdf file is the output of RTGTOOLS FORMAT. This file can be saved and reused further. + +
    + +Reusable reference files are saved in this directory. + ### MultiQC
    diff --git a/docs/usage.md b/docs/usage.md index 09406b8..6d6749a 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -27,16 +27,15 @@ test2,test2.vcf,gatk,small test3,test3.vcf.gz,cnvkit,cnv ``` -| Column | Description | -| ---------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `id` | Custom id name per test vcf. This entry will be identical. | -| `test_vcf` | The VCF file to use as benchmarking test input. The same file can be used in more than one row. File can be either vcf or vcf.gz. | -| `caller` | Variant caller method used to generate test VCF file. There can be more than one test vcf for the same caller. For unknown caller use 'unknown' | -| `vartype` | Variant type to apply benchmarking. Variant type can be only one of these: small, sv, snv, indel and cnv. | +| Column | Description | +| ---------- | ----------------------------------------------------------------------------------------------------------------------------------------------- | +| `id` | Custom id name per test vcf. This entry will be identical. | +| `test_vcf` | The VCF file to use as benchmarking test input. The same file can be used in more than one row. File can be either vcf or vcf.gz. | +| `caller` | Variant caller method used to generate test VCF file. There can be more than one test vcf for the same caller. For unknown caller use 'unknown' | +| `vartype` | Variant type to apply benchmarking. Variant type can be only one of these: small, sv, snv, indel and cnv. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. - ## Defining Truth VCF and High confidence BED files The following parameters has to be defined for each type of benchmarking analysis. The following parameters defined the exact paths to the truth files: @@ -45,26 +44,26 @@ The following parameters has to be defined for each type of benchmarking analysi - `--analysis`: The type of analysis to perform: `germline` or `somatic`. - `--method`: List the benchmarking methods to apply. By default all available tools will be applied according to the variant types provided. Available tools: `truvari, svanalyzer, happy, sompy, rtgtools, wittyer`. -*Small variant benchmarking:* +_Small variant benchmarking:_ - `--truth_small`: Path to the golden set VCF files combined for SNVs and indels, required for germline benchmarking (vcf or vcf.gz) -- `--high_conf_small`: Path to the high confidence BED files for SNVs and indels, required for germline benchmarking (bed or bed.gz) +- `--high_conf_small`: Path to the high confidence BED files for SNVs and indels, required for germline benchmarking (bed or bed.gz) - `--truth_snv`: Path to the golden set VCF files for SNVs, required for somatic benchmarking (vcf or vcf.gz) - `--high_conf_snv`: Path to the high confidence BED files for SNVs, required for somatic benchmarking (bed or bed.gz) - `--truth_indel`: Path to the golden set VCF files for indels, required for somatic benchmarking (vcf or vcf.gz) - `--high_conf_indel`: Path to the high confidence BED files for indels, required for somatic benchmarking (bed or bed.gz) -*Structural variant benchmarking:* +_Structural variant benchmarking:_ - `--truth_sv`: Path to the golden set VCF files for SVs, required for germline and somatic benchmarking (vcf or vcf.gz) - `--high_conf_sv`: Path to the high confidence BED files for SVs, required for germline and somatic benchmarking (bed or bed.gz) -*Copy Number Variation benchmarking:* +_Copy Number Variation benchmarking:_ - `--truth_cnv`: Path to the golden set VCF files for CNVs, required for germline and somatic benchmarking (vcf or vcf.gz) - `--high_conf_cnv`: Path to the high confidence BED files for CNVs, required for germline and somatic benchmarking (bed or bed.gz) -*Using truth.config* +_Using truth.config_ `conf/truth.config` file contains some readily available truth files for germline and somatic analysis. In order to activate usage one has to @@ -83,9 +82,10 @@ Note: these two files are also provided under `itruth.config`. An example usage ## Standardization and normalization parameters -Consistent formatting and alignment of variants in test and truth VCF files for accurate comparison is controlled by *sv_standardization* and *preprocesses*. +Consistent formatting and alignment of variants in test and truth VCF files for accurate comparison is controlled by _sv_standardization_ and _preprocesses_. - `--sv_standardization`: The standardization methods to perform on the input files. Should be a comma-separated list of one or more of the following options: `homogenize,svync`. + - `homogenize`: makes use of [variant-extractor](https://github.com/EUCANCan/variant-extractor) - `svync`: makes use of [svync](https://github.com/nvnieuwk/svync) @@ -99,7 +99,6 @@ Consistent formatting and alignment of variants in test and truth VCF files for If the input test vcf contains more than one sample, then user has to define which sample name to use. `subsample` will added to the samplesheet as an additional column as follows: - ```csv title="samplesheet.csv" id,test_vcf,caller,vartype,subsample test1,test1.vcf.gz,delly,sv,"TUMOR" @@ -109,12 +108,59 @@ test3,test3.vcf.gz,cnvkit,cnv, Note that, this option can be inevitable for somatic analysis since most of the callers reports both normal and tumor genotypes in the same vcf file. +## Optional benchmarking parameters + +Benchmarking parameters may vary between the tools and for callers. In order to use the same parameters for all callers be sure to write the same value for all. If noting provided, deafault values will be used. + +_SVbenchmark_ + +```csv title="samplesheet.csv" +id,test_vcf,caller,vartype,normshift,normdist,normsizediff,maxdist +test1,test1.vcf.gz,delly,sv,0.7,0.7,0.7,100000 +test2,test2.vcf,gatk,sv,0.6,0.5,0.7,110000 +``` + +- `normshift`: Has to be between 0-1. Disallow matches if alignments between alternate alleles have normalized shift greater than normshift (default 0.2) +- `normdist`: Has to be between 0-1. Disallow matches if alternate alleles have normalized edit distance greater than normdist (default 0.2) +- `normsizediff`: Has to be between 0-1. Disallow matches if alternate alleles have normalized size difference greater than normsizediff (default 0.2) +- `maxdist`: Disallow matches if positions of two variants are more than maxdist bases from each other (default 100,000) + +_Truvari_ + +```csv title="samplesheet.csv" +id,test_vcf,caller,vartype,pctsize,pctseq,pctovl,refdist,chunksize,dup_to_ins,typeignore +test1,test1.vcf.gz,delly,sv,0.7,0.7,0.7,100000,50000,true,true +test2,test2.vcf,gatk,sv,0.6,0.5,0.7,110000,40000,false,true +``` + +- `pctsize`: Has to be between 0-1. Ratio of min(base_size, comp_size)/max(base_size, comp_size) +- `pctseq`: Has to be between 0-1. Edit distance ratio between the REF/ALT haplotype sequences of base and comparison call. Turn it off (0) for no sequence comparison. +- `pctovl`: Has to be between 0-1. Ratio of two calls' (overlapping bases)/(longest span) +- `refdist`: Maximum distance comparison calls must be within from base call's start/end +- `chunksize`: Create chunks of all calls overlapping within ±chunksize basepairs +- `dup_to_ins`: Converts DUP to INS type (boolean) +- `typeignore`: Ignore SVTYPE matching (boolean) + +_Wittyer_ + +```csv title="samplesheet.csv" +id,test_vcf,caller,vartype,bpDistance,percentThreshold,absoluteThreshold,maxMatches,evaluationmode +test1,test1.vcf.gz,delly,sv,200,0.5,17000,100,sc +test2,test2.vcf,gatk,sv,100,0.5,11000,-1,cts +``` + +- `bpDistance`: Upper bound of boundary distance when comparing truth and query. By default it is 500bp for all types except for Insertions, which are 100bp.Please note that if you set this value in the command line, it overrides all the defaults, so Insertions and other types will have the same bpd. +- `percentThreshold`: This is used for percentage thresholding. For CopyNumberTandemRepeats, this determines how large of a RepeatUnitCount (RUC) threshold to use for large tandem repeats. For all other SVs, in order to match between query and truth, the distance between boundaries should be within a number thats proportional to total SV (default 0.25) +- `absoluteThreshold`: This is used for absolute thresholding. For CopyNumberTandemRepeats, this determines how large of a RepeatUnitCount (RUC) threshold to use. For all other SVs, this is the upper bound of boundary distance when comparing truth and query. (default 10000) +- `maxMatches`: axMatches is a wittyer parameter. This is used for matching behaviour. Negative value means to match any number (for large SVs it is not recommended). +- `evaluationmode`: It is by default requires genotype matching. simpleCounting:sc, CrossTypeAndSimpleCounting:cts, genotypematch:d + ## Filtering parameters - `--exclude_expression`: Use [bcftools expressions](https://samtools.github.io/bcftools/bcftools.html#expressions) to exclude variants. Default:null - `--include_expression`: Use [bcftools expressions](https://samtools.github.io/bcftools/bcftools.html#expressions) to include variants. Default:null -*Parameters applicable only to Structural Variants* +_Parameters applicable only to Structural Variants_ - `--min_sv_size`: Minimum SV size of variants to benchmark, 0 to disable , Default:30 - `--max_sv_size`: Maximum SV size of variants to benchmark, -1 to disable , Default:-1 diff --git a/nextflow_schema.json b/nextflow_schema.json index 4f1518d..be1069a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -282,6 +282,15 @@ "description": "Path to the ranaming chromosomes for lifting over.", "help_text": "This parameter is *mandatory* if `--liftover` is true and --genome is not specified and --itruth is false. A rename can be found under assets/rename_contigs or can be used together with --genome.", "fa_icon": "far fa-file-code" + }, + "dictionary": { + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.dict$", + "description": "The dictionary file is required ofr liftover process. It has to be .dict of genome file used in the workflow. ", + "help_text": "This will be automatically generated by the pipeline when missing", + "fa_icon": "fas fa-file-csv" } } }, @@ -289,8 +298,8 @@ "title": "Institutional config options", "type": "object", "fa_icon": "fas fa-university", - "description": "Parameters used to describe centralised config profiles. These should not be edited.", - "help_text": "The centralised nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", + "description": "Parameters used to describe centralized config profiles. These should not be edited.", + "help_text": "The centralized nf-core configuration profiles use a handful of pipeline parameters to describe themselves. This information is then printed to the Nextflow log when you run a pipeline. You should not need to change these values when you run a pipeline.", "properties": { "custom_config_version": { "type": "string", diff --git a/subworkflows/local/liftover_vcfs_truth.nf b/subworkflows/local/liftover_vcfs_truth.nf index 867589b..c6e030d 100644 --- a/subworkflows/local/liftover_vcfs_truth.nf +++ b/subworkflows/local/liftover_vcfs_truth.nf @@ -18,21 +18,25 @@ workflow LIFTOVER_VCFS_TRUTH { fasta // reference channel [val(meta), ref.fa] chain // chain channel [val(meta), chain.gz] rename_chr // reference channel [val(meta), chrlist.txt] + dictionary // reference channel [val(meta), genome.dict] main: versions = Channel.empty() //prepare dict file for liftover of vcf files - PICARD_CREATESEQUENCEDICTIONARY( - fasta - ) - versions = versions.mix(PICARD_CREATESEQUENCEDICTIONARY.out.versions.first()) + if (!params.dictionary){ + PICARD_CREATESEQUENCEDICTIONARY( + fasta + ) + dictionary = PICARD_CREATESEQUENCEDICTIONARY.out.reference_dict + versions = versions.mix(PICARD_CREATESEQUENCEDICTIONARY.out.versions.first()) + } // Use picard liftovervcf tool to convert vcfs PICARD_LIFTOVERVCF( truth_ch, - PICARD_CREATESEQUENCEDICTIONARY.out.reference_dict, + dictionary, fasta, chain ) diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 8f21443..6278699 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -18,6 +18,7 @@ workflow PREPARE_VCFS_TRUTH { fai // reference channel [val(meta), ref.fa.fai] chain // reference channel [val(meta), chain.gz] rename_chr // reference channel [val(meta), chrlist.txt] + dictionary // reference channel [val(meta), genome.dict] main: @@ -31,7 +32,8 @@ workflow PREPARE_VCFS_TRUTH { high_conf_ch, fasta, chain, - rename_chr + rename_chr, + dictionary ) versions = versions.mix(LIFTOVER_VCFS_TRUTH.out.versions.first()) truth_ch = LIFTOVER_VCFS_TRUTH.out.vcf_ch diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index e768c94..8dd6cf8 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -123,12 +123,16 @@ workflow VARIANTBENCHMARKING { // read chainfile, liftover genome and rename chr files if liftover is true chain = Channel.empty() rename_chr = Channel.empty() + dictionary = Channel.empty() if (params.liftover){ chain = params.chain ? Channel.fromPath(params.chain, checkIfExists: true).map{ bed -> tuple([id: bed.getSimpleName()], bed) }.collect() : Channel.empty() - rename_chr = params.rename_chr ? Channel.fromPath(params.rename_chr, checkIfExists: true).map{ txt -> tuple([txt: txt.getSimpleName()], txt) }.collect() + rename_chr = params.rename_chr ? Channel.fromPath(params.rename_chr, checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() + : Channel.empty() + + dictionary = params.dictionary ? Channel.fromPath(params.dictionary, checkIfExists: true).map{ dict -> tuple([id: dict.getSimpleName()], dict) }.collect() : Channel.empty() } // PREPROCESSES @@ -183,7 +187,8 @@ workflow VARIANTBENCHMARKING { fasta, fai, chain, - rename_chr + rename_chr, + dictionary ) high_conf_ch = PREPARE_VCFS_TRUTH.out.high_conf_ch ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) From 7d11446c65113183abd4d822a0d21caba2d7b859 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 12 Aug 2024 11:35:48 +0000 Subject: [PATCH 165/292] add dictionary --- nextflow.config | 11 ++++++----- 1 file changed, 6 insertions(+), 5 deletions(-) diff --git a/nextflow.config b/nextflow.config index 3246392..a7fea87 100644 --- a/nextflow.config +++ b/nextflow.config @@ -13,9 +13,10 @@ params { // Input options input = null outdir = "results" - sample = null // "HG002,SEQC2" - analysis = null // "germline,somatic" - sdf = null // The SDF format file needed by rtgtools + sample = null + analysis = null + sdf = null + dictionary = null liftover = false // Benchmarking method @@ -24,8 +25,8 @@ params { // Preprocess spesific parameters // normalization includes braking down multiallelic samples -m any // deduplication removes one of the variants in the same position - preprocess = "" //normalization,deduplication,prepy,filter_contigs - sv_standardization = "" // homogenize,svync + preprocess = "" + sv_standardization = "" // Filtering parameters // minsize effects both truvari and variantbenchmarkingmark in different ways! svbechmark filters both base and comp calls From 0c2bd7304dd2b154694fe18236bac73d46ec0225 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 13 Aug 2024 15:55:56 +0200 Subject: [PATCH 166/292] add images --- README.md | 17 +- docs/images/GHGA_short_Logo_orange.png | Bin 0 -> 22099 bytes docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/images/variantbenchmarking_metromap.png | Bin 0 -> 217758 bytes docs/images/variantbenchmarking_metromap.svg | 3507 ++++++++++++++++++ 7 files changed, 3522 insertions(+), 2 deletions(-) create mode 100644 docs/images/GHGA_short_Logo_orange.png delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png create mode 100644 docs/images/variantbenchmarking_metromap.png create mode 100644 docs/images/variantbenchmarking_metromap.svg diff --git a/README.md b/README.md index 500ca16..09120d7 100644 --- a/README.md +++ b/README.md @@ -21,6 +21,12 @@ **nf-core/variantbenchmarking** is designed to evaluate and validate the accuracy of variant calling methods in genomic research especially for Human data. Initially, the pipeline is tuned well for available gold standard truth sets (for example, Genome in a Bottle and SEQC2 samples) but it can be used to compare any two variant calling results. The workflow provides benchmarking tools for small variants including SNVs and INDELs, Structural Variants (SVs) and Copy Number Variations (CNVs) for germline and somatic analysis. +The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! + +

    + +

    + The workflow involves several key processes to ensure reliable and reproducible results as follows: ### Standardization and normalization of variants: @@ -149,8 +155,15 @@ nf-core/variantbenchmarking was originally written by Kübra Narcı ([@kubranarc We thank the following people for their extensive assistance in the development of this pipeline: -Nicolas Vannieuwkerke ([@nvnienwk](https://github.com/nvnieuwk)) -Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) +- Nicolas Vannieuwkerke ([@nvnienwk](https://github.com/nvnieuwk)), +- Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) + +## Acknowledgements + + + GHGA + + ## Contributions and Support diff --git a/docs/images/GHGA_short_Logo_orange.png b/docs/images/GHGA_short_Logo_orange.png new file mode 100644 index 0000000000000000000000000000000000000000..180996f662a1452eba41205943f9fb76d54106d7 GIT binary patch literal 22099 zcmeFZXH=70*ESjwiVA`XQba&(5UG*g0&YY^nt;+f(xe4KhXC0qC@3f(Na!7;gY*_f 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    + + nf-core/variantbenchmarking Pipeline Report + + +
    + - +

    nf-core/variantbenchmarking ${version}

    +

    Run Name: $runName

    -

    nf-core/variantbenchmarking ${version}

    -

    Run Name: $runName

    - -<% if (!success){ - out << """ -
    -

    nf-core/variantbenchmarking execution completed unsuccessfully!

    + <% if (!success){ out << """ +
    +

    nf-core/variantbenchmarking execution completed unsuccessfully!

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    The full error message was:

    - 
    ${errorReport}
    -
    - """ -} else { - out << """ -
    + 
    ${errorReport}
    +
    + """ } else { out << """ +
    nf-core/variantbenchmarking execution completed successfully! -
    - """ -} -%> +
    + """ } %> -

    The workflow was completed at $dateComplete (duration: $duration)

    -

    The command used to launch the workflow was as follows:

    -
    $commandLine
    +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    + 
    +$commandLine
    -

    Pipeline Configuration:

    -

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Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 13 Aug 2024 14:01:46 +0000 Subject: [PATCH 167/292] [automated] Fix code linting --- README.md | 1 - 1 file changed, 1 deletion(-) diff --git a/README.md b/README.md index 09120d7..380e62b 100644 --- a/README.md +++ b/README.md @@ -164,7 +164,6 @@ We thank the following people for their extensive assistance in the development GHGA - ## Contributions and Support If you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md). From f6403ce05d9c97ba4ebb0b97f2978aad97ef7dd1 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 16 Aug 2024 08:53:35 +0000 Subject: [PATCH 168/292] rearrange test config files and samplesheets --- assets/samplesheet.csv | 9 +- assets/samplesheet_HG002_hg37.csv | 11 - assets/samplesheet_HG002_hg37_full.csv | 5 - assets/samplesheet_HG002_hg38.csv | 5 - assets/samplesheet_HG002_hg38_full.csv | 5 - assets/samplesheet_SEQC2.csv | 6 - assets/samplesheet_full.csv | 11 +- assets/samplesheet_sarek_somatic_test.csv | 7 - assets/samplesheet_small.csv | 4 - ...sarek_test.csv => samplesheet_somatic.csv} | 0 conf/test.config | 8 +- conf/test_data.config | 347 ------------------ conf/test_full.config | 42 +-- conf/test_hg19.config | 41 --- conf/test_hg37.config | 44 --- conf/test_hg37_full.config | 44 --- conf/test_hg38.config | 44 --- conf/test_liftover.config | 35 +- conf/test_small.config | 38 -- ...g38_somatic.config => test_somatic.config} | 10 +- conf/truth.config | 2 +- nextflow.config | 11 +- tests/main.nf.test | 47 +++ tests/nextflow.config | 27 ++ workflows/variantbenchmarking.nf | 1 - 25 files changed, 132 insertions(+), 672 deletions(-) delete mode 100644 assets/samplesheet_HG002_hg37.csv delete mode 100644 assets/samplesheet_HG002_hg37_full.csv delete mode 100644 assets/samplesheet_HG002_hg38.csv delete mode 100644 assets/samplesheet_HG002_hg38_full.csv delete mode 100644 assets/samplesheet_SEQC2.csv delete mode 100644 assets/samplesheet_sarek_somatic_test.csv delete mode 100644 assets/samplesheet_small.csv rename assets/{samplesheet_SEQC2_sarek_test.csv => samplesheet_somatic.csv} (100%) delete mode 100644 conf/test_data.config delete mode 100644 conf/test_hg19.config delete mode 100644 conf/test_hg37.config delete mode 100644 conf/test_hg37_full.config delete mode 100644 conf/test_hg38.config delete mode 100644 conf/test_small.config rename conf/{test_hg38_somatic.config => test_somatic.config} (86%) create mode 100644 tests/main.nf.test create mode 100644 tests/nextflow.config diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index cd42d3b..af2c0cd 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,3 +1,6 @@ -id,test_vcf,caller,vartype -haplotypecaller1,"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",mutect,sv -unknown1,"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz",unknown,sv +id,test_vcf,caller,vartype,pctsize,pctseq,pctovl,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,evaluationmode +manta1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts +merged1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts +dragen1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts +strelka1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.strelka.variants.chr21.vcf.gz,strelka,small,,,,,,,,,,,, +bcftools1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.bcftools.chr21.vcf.gz,bcftools,small,,,,,,,,,,,, diff --git a/assets/samplesheet_HG002_hg37.csv b/assets/samplesheet_HG002_hg37.csv deleted file mode 100644 index f18b9fa..0000000 --- a/assets/samplesheet_HG002_hg37.csv +++ /dev/null @@ -1,11 +0,0 @@ -id,test_vcf,caller,vartype,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode -delly1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_delly_SV_hg19.chr21.vcf.gz",delly,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -lumpy1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_lumpy_SV_hg19.sorted.vcf.gz",lumpy,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -manta1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_manta_SV_hg19_genotype.chr21.vcf.gz",manta,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -svaba1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002.svaba.germline.sv.chr21.vcf.gz",svaba,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -sbg1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002-NA24385-50x.union_170414.split.chr21.vcf.gz",sbg_graph,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -gatk1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002.jointVC.filter.chr21.vcf.gz",gatk_joint,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -freebayes1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_ALLCHROM_hs37d5_novoalign_Ilmn150bp300X_FB.chr21.vcf.gz",freebayes,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -assemlytics1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/hg002.Assemblytics_structural_variants.sorted.chr21.vcf.gz",assemblytics,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts -sbg2,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002-NA24385-50x.union_170414.split.chr21.vcf.gz",sbg_graph,cnv,,,,,,,true,true,0.3,0,0,cts -lumpy2,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_lumpy_SV_hg19.sorted.vcf.gz",lumpy,cnv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts diff --git a/assets/samplesheet_HG002_hg37_full.csv b/assets/samplesheet_HG002_hg37_full.csv deleted file mode 100644 index ca2a6b1..0000000 --- a/assets/samplesheet_HG002_hg37_full.csv +++ /dev/null @@ -1,5 +0,0 @@ -id,test_vcf,caller,vartype,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode,subsample -test1,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/manta_GIABv0.6/diploidSV_PASS_DUPtoINS.vcf.gz",manta,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, -test2,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/delly_GIABv0.6/norm_recall_HG002.merged__filt_DUPtoINS.vcf.gz",delly,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, -test4,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/SevenBridges_GraphGATKRefine_05052017/HG002-NA24385-50x.union_170414.split.vcf.gz",graph,small,,,,,,,,,,,,, -test5,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/OsloUniversityHospital_Exome_GATK_jointVC_11242015/HG002-HG003-HG004.jointVC.filter.vcf",gatk,small,,,,,,,,,,,,,"Sample_Diag-excap51-HG002-EEogPU" diff --git a/assets/samplesheet_HG002_hg38.csv b/assets/samplesheet_HG002_hg38.csv deleted file mode 100644 index fa7095e..0000000 --- a/assets/samplesheet_HG002_hg38.csv +++ /dev/null @@ -1,5 +0,0 @@ -id,test_vcf,caller,vartype,pctsize,pctseq,pctovl,refdist,chunksize,normshift,normdist,normsizediff,maxdist -lumpy1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.chr21.vcf.gz,lumpy,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000 -manta1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000 -merged1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000 -dragen1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000 diff --git a/assets/samplesheet_HG002_hg38_full.csv b/assets/samplesheet_HG002_hg38_full.csv deleted file mode 100644 index bd68c9f..0000000 --- a/assets/samplesheet_HG002_hg38_full.csv +++ /dev/null @@ -1,5 +0,0 @@ -id,test_vcf,caller,vartype,pctsize,pctseq,pctovl,refdist,chunksize,normshift,normdist,normsizediff,maxdist -test11,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/BU_GRCh38_SVs_06252018/ajtrio.lumpy.svtyper.HG002.md.sorted.recal.vcf.gz",lumpy,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000 -test22,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/BU_GRCh38_SVs_06252018/HG002.gatk.raw.snps.indels.vcf.gz",gatk,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000 -test3,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/BU_GRCh38_SVs_06252018/manta.HG002.vcf.gz",manta,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000 -test4,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/BU_GRCh38_SVs_06252018/HG002.gatk.raw.snps.indels.vcf.gz",gatk,small,,,,,,,,, diff --git a/assets/samplesheet_SEQC2.csv b/assets/samplesheet_SEQC2.csv deleted file mode 100644 index cd69f8f..0000000 --- a/assets/samplesheet_SEQC2.csv +++ /dev/null @@ -1,6 +0,0 @@ -id,test_vcf,caller,vartype -mutect1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/test/hg38/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-MuTect2.chr21.vcf.gz,mutect2,small -strelka1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/test/hg38/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-Strelka.snv.chr21.vcf.gz,strelka,snv -strelka2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/test/hg38/WGS.bwa.dedup-IL_T_1_vs_IL_N_1-Strelka.indel.chr21.vcf.gz,strelka,indel -dkfz1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/test/hg38/snvs_SEQC2_LL2_somatic_functional_snvs_conf_8_to_10.std.sorted.chr21.vcf.gz,dkfz,snv -dkfz2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/test/hg38/indel_WES_LL_somatic_indels_conf_8_to_10.std.sorted.chr21.vcf.gz,dkfz,indel diff --git a/assets/samplesheet_full.csv b/assets/samplesheet_full.csv index 7d26175..ca2a6b1 100644 --- a/assets/samplesheet_full.csv +++ b/assets/samplesheet_full.csv @@ -1,6 +1,5 @@ -id,test_vcf,caller,vartype,pctsize,pctseq,pctovl,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,evaluationmode -manta1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -merged1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -dragen1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -strelka1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/HG002.strelka.variants.chr21.vcf.gz,strelka,small,,,,,,,,,,,, -bcftools1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/HG002.bcftools.chr21.vcf.gz,bcftools,small,,,,,,,,,,,, +id,test_vcf,caller,vartype,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode,subsample +test1,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/manta_GIABv0.6/diploidSV_PASS_DUPtoINS.vcf.gz",manta,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, +test2,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/delly_GIABv0.6/norm_recall_HG002.merged__filt_DUPtoINS.vcf.gz",delly,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, +test4,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/SevenBridges_GraphGATKRefine_05052017/HG002-NA24385-50x.union_170414.split.vcf.gz",graph,small,,,,,,,,,,,,, +test5,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/OsloUniversityHospital_Exome_GATK_jointVC_11242015/HG002-HG003-HG004.jointVC.filter.vcf",gatk,small,,,,,,,,,,,,,"Sample_Diag-excap51-HG002-EEogPU" diff --git a/assets/samplesheet_sarek_somatic_test.csv b/assets/samplesheet_sarek_somatic_test.csv deleted file mode 100644 index 2efe9ed..0000000 --- a/assets/samplesheet_sarek_somatic_test.csv +++ /dev/null @@ -1,7 +0,0 @@ -id,test_vcf,caller,vartype -freebayes,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.freebayes.chr21.vcf.gz,freebayes,small -manta,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.manta.somatic_sv.chr21.vcf.gz,manta,sv -strelka1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_indels.chr21.vcf.gz,strelka,indel -strelka2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.chr21.vcf.gz,strelka,snv -tiddit1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.tiddit.tumor.chr21.vcf.gz,tiddit,small -tiddit2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.tiddit_sv_merge.chr21.vcf.gz,tiddit,sv diff --git a/assets/samplesheet_small.csv b/assets/samplesheet_small.csv deleted file mode 100644 index 027ff32..0000000 --- a/assets/samplesheet_small.csv +++ /dev/null @@ -1,4 +0,0 @@ -id,test_vcf,caller,vartype -haplotypecaller1,"https://raw.githubusercontent.com/nf-core/test-datasets/modules/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz",haplotypecaller,small -strelka1,"https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/HG002.strelka.variants.chr21.vcf.gz",strelka,small - diff --git a/assets/samplesheet_SEQC2_sarek_test.csv b/assets/samplesheet_somatic.csv similarity index 100% rename from assets/samplesheet_SEQC2_sarek_test.csv rename to assets/samplesheet_somatic.csv diff --git a/conf/test.config b/conf/test.config index 522f3eb..bf6c831 100644 --- a/conf/test.config +++ b/conf/test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, --outdir + nextflow run nf-core/variantbenchmarking -profile test, ---------------------------------------------------------------------------------------- */ @@ -15,13 +15,13 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = '100.GB' + max_cpus = 2 + max_memory = '6.5GB' max_time = '8.h' // Input data sample = "HG002" - input = "assets/samplesheet_full.csv" + input = "assets/samplesheet.csv" outdir = "results" // Genome references diff --git a/conf/test_data.config b/conf/test_data.config deleted file mode 100644 index 6a98dd0..0000000 --- a/conf/test_data.config +++ /dev/null @@ -1,347 +0,0 @@ -// README: -// https://github.com/nf-core/test-datasets/blob/modules/README.md - -params { - // Base directory for test data - test_data_base = "https://raw.githubusercontent.com/nf-core/test-datasets/modules" - - test_data { - 'homo_sapiens' { - 'genome' { - genome_elfasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.elfasta" - genome_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta" - genome_fasta_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.fai" - genome_fasta_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz" - genome_fasta_gz_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz.fai" - genome_fasta_gz_gzi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.fasta.gz.gzi" - genome_strtablefile = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_strtablefile.zip" - genome_dict = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.dict" - genome_gff3 = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.gff3" - genome_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.gtf" - genome_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.interval_list" - genome_multi_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.multi_intervals.bed" - genome_blacklist_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.blacklist_intervals.bed" - genome_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.sizes" - genome_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed" - genome_header = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.header" - genome_bed_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed.gz" - genome_bed_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.bed.gz.tbi" - genome_elsites = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.elsites" - transcriptome_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/transcriptome.fasta" - genome2_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome2.fasta" - genome_chain_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.chain.gz" - genome_annotated_interval_tsv = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.annotated_intervals.tsv" - genome_mt_gb = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.NC_012920_1.gb" - genome_preprocessed_count_tsv = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.preprocessed_intervals.counts.tsv" - genome_preprocessed_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.preprocessed_intervals.interval_list" - genome_ploidy_model = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.ploidy_model.tar.gz" - genome_ploidy_calls = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.ploidy_calls.tar.gz" - genome_germline_cnv_model = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.germline_cnv_model.tar.gz" - genome_germline_cnv_calls = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome.germline_cnv_calls.tar.gz" - genome_motifs = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_motifs.txt" - genome_config = "${params.test_data_base}/data/genomics/homo_sapiens/genome/genome_config.json" - - genome_1_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr1/genome.fasta.gz" - genome_1_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr1/genome.gtf" - - genome_21_sdf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome_sdf.tar.gz" - genome_21_fasta = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta" - genome_21_fasta_fai = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai" - genome_21_gencode_gtf = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chr21_gencode.gtf" - genome_21_dict = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.dict" - genome_21_sizes = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.sizes" - genome_21_interval_list = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/genome.interval_list" - genome_21_annotated_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/annotated.bed" - genome_21_multi_interval_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed" - genome_21_multi_interval_antitarget_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.antitarget.bed" - genome_21_multi_interval_bed_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed.gz" - genome_21_multi_interval_bed_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed.gz.tbi" - genome_21_chromosomes_dir = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chromosomes.tar.gz" - genome_21_reference_cnn = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/reference_chr21.cnn" - genome_21_eigenstrat_snp = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/chr_21.snp" - genome_21_stitch_posfile = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/sequence/dbsnp_138.hg38.first_10_biallelic_sites.tsv" - - dbsnp_146_hg38_elsites = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.elsites" - dbsnp_146_hg38_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz" - dbsnp_146_hg38_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/dbsnp_146.hg38.vcf.gz.tbi" - gnomad_r2_1_1_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz" - gnomad_r2_1_1_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1.vcf.gz.tbi" - mills_and_1000g_indels_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz" - mills_and_1000g_indels_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/mills_and_1000G.indels.vcf.gz.tbi" - syntheticvcf_short_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.vcf.gz" - syntheticvcf_short_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.vcf.gz.tbi" - syntheticvcf_short_score = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/syntheticvcf_short.score" - gnomad_r2_1_1_sv_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD.r2.1.1-sv.vcf.gz" - gnomad2_r2_1_1_sv_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/gnomAD2.r2.1.1-sv.vcf.gz" - - hapmap_3_3_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/hapmap_3.3.hg38.vcf.gz" - hapmap_3_3_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/hapmap_3.3.hg38.vcf.gz.tbi" - res_1000g_omni2_5_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_omni2.5.hg38.vcf.gz" - res_1000g_omni2_5_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_omni2.5.hg38.vcf.gz.tbi" - res_1000g_phase1_snps_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_phase1.snps.hg38.vcf.gz" - res_1000g_phase1_snps_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/1000G_phase1.snps.hg38.vcf.gz.tbi" - dbsnp_138_hg38_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbsnp_138.hg38.vcf.gz" - dbsnp_138_hg38_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbsnp_138.hg38.vcf.gz.tbi" - gnomad_r2_1_1_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/gnomAD.r2.1.1.vcf.gz" - gnomad_r2_1_1_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/gnomAD.r2.1.1.vcf.gz.tbi" - mills_and_1000g_indels_21_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/mills_and_1000G.indels.hg38.vcf.gz" - mills_and_1000g_indels_21_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/mills_and_1000G.indels.hg38.vcf.gz.tbi" - haplotype_map = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/haplotype_map.txt" - dbNSFP_4_1a_21_hg38_txt_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbNSFP4.1a.21.txt.gz" - dbNSFP_4_1a_21_hg38_txt_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/dbNSFP4.1a.21.txt.gz.tbi" - ngscheckmate_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/chr21/germlineresources/SNP_GRCh38_hg38_wChr.bed" - - index_salmon = "${params.test_data_base}/data/genomics/homo_sapiens/genome/index/salmon" - repeat_expansions = "${params.test_data_base}/data/genomics/homo_sapiens/genome/loci/repeat_expansions.json" - justhusky_ped = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky.ped" - justhusky_minimal_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky_minimal.vcf.gz" - justhusky_minimal_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/ped/justhusky_minimal.vcf.gz.tbi" - - vcfanno_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/vcfanno/vcfanno_grch38_module_test.tar.gz" - vcfanno_toml = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vcf/vcfanno/vcfanno.toml" - updsites_bed = "${params.test_data_base}/data/genomics/homo_sapiens/genome/updsites.bed" - - prg_input = "${params.test_data_base}/data/genomics/homo_sapiens/genome/PRG_test.zip" - crispr_functional_counts = "${params.test_data_base}/data/genomics/homo_sapiens/genome/tsv/functional_genomics_counts.tsv" - crispr_functional_library = "${params.test_data_base}/data/genomics/homo_sapiens/genome/tsv/library_functional_genomics.tsv" - - vep_cache = "${params.test_data_base}/data/genomics/homo_sapiens/genome/vep.tar.gz" - affy_array_samplesheet = "${params.test_data_base}/data/genomics/homo_sapiens/array_expression/GSE38751.csv" - affy_array_celfiles_tar = "${params.test_data_base}/data/genomics/homo_sapiens/array_expression/GSE38751_RAW.tar" - - } - 'pangenome' { - pangenome_fa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa" - pangenome_fa_bgzip = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz" - pangenome_fa_bgzip_fai = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz.fai" - pangenome_fa_bgzip_gzi = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.fa.gz.gzi" - pangenome_paf = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.paf" - pangenome_paf_gz = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.paf.gz" - pangenome_seqwish_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.seqwish.gfa" - pangenome_smoothxg_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.smoothxg.gfa" - pangenome_gfaffix_gfa = "${params.test_data_base}/data/pangenomics/homo_sapiens/pangenome.gfaffix.gfa" - 'odgi' { - pangenome_og = "${params.test_data_base}/data/pangenomics/homo_sapiens/odgi/pangenome.og" - pangenome_lay = "${params.test_data_base}/data/pangenomics/homo_sapiens/odgi/pangenome.lay" - } - } - 'illumina' { - test_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam" - test_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.sorted.bam.bai" - test_paired_end_name_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.name.sorted.bam" - test_paired_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam" - test_paired_end_markduplicates_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.bam.bai" - test_paired_end_markduplicates_sorted_referencesn_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.markduplicates.sorted.referencesn.txt" - test_paired_end_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam" - test_paired_end_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.paired_end.recalibrated.sorted.bam.bai" - test_paired_end_umi_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_consensus.bam" - test_paired_end_umi_converted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_converted.bam" - test_paired_end_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_grouped.bam" - test_paired_end_umi_histogram_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_histogram.txt" - test_paired_end_umi_unsorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.umi_unsorted.bam" - test_paired_end_umi_unsorted_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.unsorted_tagged.bam" - test_paired_end_hla = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.bam" - test_paired_end_hla_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam" - test_paired_end_hla_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/example_hla_pe.sorted.bam.bai" - test_rna_paired_end_sorted_chr6_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.chr6.bam" - test_rna_paired_end_sorted_chr6_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test.rna.paired_end.sorted.chr6.bam.bai" - - test2_paired_end_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam" - test2_paired_end_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam.bai" - test2_paired_end_name_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.name.sorted.bam" - test2_paired_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.markduplicates.sorted.bam" - test2_paired_end_markduplicates_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.markduplicates.sorted.bam.bai" - test2_paired_end_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam" - test2_paired_end_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test2.paired_end.recalibrated.sorted.bam.bai" - test2_paired_end_umi_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_consensus.bam" - test2_paired_end_umi_converted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_converted.bam" - test2_paired_end_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_grouped.bam" - test2_paired_end_umi_histogram_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_histogram.txt" - test2_paired_end_umi_unsorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.umi_unsorted.bam" - test2_paired_end_umi_unsorted_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test2.paired_end.unsorted_tagged.bam" - test_paired_end_duplex_umi_unmapped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_unmapped.bam" - test_paired_end_duplex_umi_mapped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_mapped.bam" - test_paired_end_duplex_umi_mapped_tagged_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_mapped_tagged.bam" - test_paired_end_duplex_umi_grouped_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_grouped.bam" - test_paired_end_duplex_umi_duplex_consensus_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/umi/test.paired_end.duplex_umi_duplex_consensus.bam" - - mitochon_standin_recalibrated_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam" - mitochon_standin_recalibrated_sorted_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/mitochon_standin.recalibrated.sorted.bam.bai" - test_illumina_mt_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test_illumina_mt.bam" - test_illumina_mt_bam_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test_illumina_mt.bam.bai" - - test3_single_end_markduplicates_sorted_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/test3.single_end.markduplicates.sorted.bam" - - read_group_settings_txt = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bam/read_group_settings.txt" - - test_paired_end_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram" - test_paired_end_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai" - test_paired_end_markduplicates_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.markduplicates.sorted.cram" - test_paired_end_markduplicates_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.markduplicates.sorted.cram.crai" - test_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram" - test_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test.paired_end.recalibrated.sorted.cram.crai" - - test2_paired_end_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.sorted.cram" - test2_paired_end_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.sorted.cram.crai" - test2_paired_end_markduplicates_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.markduplicates.sorted.cram" - test2_paired_end_markduplicates_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.markduplicates.sorted.cram.crai" - test2_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram" - test2_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram.crai" - test3_paired_end_recalibrated_sorted_cram = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test3.paired_end.recalibrated.sorted.cram" - test3_paired_end_recalibrated_sorted_cram_crai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/cram/test3.paired_end.recalibrated.sorted.cram.crai" - - test_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_1.fastq.gz" - test_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_2.fastq.gz" - test_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test.umi_1.fastq.gz" - test_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test.umi_2.fastq.gz" - test2_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2_1.fastq.gz" - test2_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2_2.fastq.gz" - test2_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2.umi_1.fastq.gz" - test2_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test2.umi_2.fastq.gz" - test_rnaseq_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz" - test_rnaseq_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz" - test_paired_end_duplex_umi_1_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_duplex_umi_1.fastq.gz" - test_paired_end_duplex_umi_2_fastq_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/fastq/test_duplex_umi_2.fastq.gz" - - test_baserecalibrator_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test.baserecalibrator.table" - test2_baserecalibrator_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test2.baserecalibrator.table" - test_pileups_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test.pileups.table" - test2_pileups_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test2.pileups.table" - - test_paired_end_sorted_dragstrmodel = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_paired_end_sorted_dragstrmodel.txt" - - test_genomicsdb_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_genomicsdb.tar.gz" - test_pon_genomicsdb_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_pon_genomicsdb.tar.gz" - - test2_haplotc_ann_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz" - test2_haplotc_ann_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.ann.vcf.gz.tbi" - test_haplotc_cnn_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test_haplotcaller.cnn.vcf.gz" - test_haplotc_cnn_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test_haplotcaller.cnn.vcf.gz.tbi" - - test2_haplotc_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz" - test2_haplotc_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz.tbi" - - test2_recal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal" - test2_recal_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.recal.idx" - test2_tranches = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2.tranches" - test2_allele_specific_recal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.recal" - test2_allele_specific_recal_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.recal.idx" - test2_allele_specific_tranches = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/variantrecalibrator/test2_allele_specific.tranches" - - test_test2_paired_mutect2_calls_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz" - test_test2_paired_mutect2_calls_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz.tbi" - test_test2_paired_mutect2_calls_vcf_gz_stats = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz.stats" - test_test2_paired_mutect2_calls_f1r2_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.f1r2.tar.gz" - test_test2_paired_mutect2_calls_artifact_prior_tar_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired_mutect2_calls.artifact-prior.tar.gz" - test_test2_paired_segmentation_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired.segmentation.table" - test_test2_paired_contamination_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/test_test2_paired.contamination.table" - - test_genome_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf" - test_genome_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.gz" - test_genome_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.gz.tbi" - test_genome_vcf_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test.genome.vcf.idx" - - test_genome_vcf_ud = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.UD" - test_genome_vcf_mu = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.mu" - test_genome_vcf_bed = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/svd/test.genome.vcf.bed" - - test2_genome_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf" - test2_genome_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.gz" - test2_genome_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.gz.tbi" - test2_genome_vcf_idx = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gvcf/test2.genome.vcf.idx" - - test_genome21_indels_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.genome_21.somatic_sv.vcf.gz" - test_genome21_indels_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.genome_21.somatic_sv.vcf.gz.tbi" - - test_mpileup = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/mpileup/test.mpileup.gz" - test2_mpileup = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/mpileup/test2.mpileup.gz" - - test_broadpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/broadpeak/test.broadPeak" - test2_broadpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/broadpeak/test2.broadPeak" - - test_narrowpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/narrowpeak/test.narrowPeak" - test2_narrowpeak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/narrowpeak/test2.narrowPeak" - - test_yak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/yak/test.yak" - test2_yak = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/yak/test2.yak" - - cutandrun_bedgraph_test_1 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/cutandtag_h3k27me3_test_1.bedGraph" - cutandrun_bedgraph_test_2 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/cutandtag_igg_test_1.bedGraph" - na24385_chr22_coverage = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bedgraph/NA24385_coverage.bed" - - empty_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/empty.vcf.gz" - empty_vcf_gz_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/empty.vcf.gz.tbi" - - simulated_sv = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv.vcf.gz" - simulated_sv_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv.vcf.gz.tbi" - simulated_sv2 = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv2.vcf.gz" - simulated_sv2_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/chr21/simulated_sv2.vcf.gz.tbi" - - test_rnaseq_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test.rnaseq.vcf" - test_sv_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz" - test_sv_vcf_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/sv_query.vcf.gz.tbi" - na24385_chr22_sv_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz" - na24385_chr22_sv_vcf_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA24385_sv.vcf.gz.tbi" - genmod_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/genmod.vcf.gz" - genmod_annotate_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_annotate.vcf.gz" - genmod_models_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_models.vcf.gz" - genmod_score_vcf_gz = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/test_score.vcf.gz" - - test_mito_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/vcf/NA12878_chrM.vcf.gz" - - test_pytor = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/pytor/test.pytor" - rank_model = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/genmod/svrank_model_-v1.8-.ini" - - test_flowcell = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bcl/flowcell.tar.gz" - test_flowcell_samplesheet = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/bcl/flowcell_samplesheet.csv" - - varlociraptor_scenario = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/varlociraptor/scenario.yml" - - contig_ploidy_priors_table = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/gatk/contig_ploidy_priors_table.tsv" - - purecn_ex1_bam = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1.bam" - purecn_ex1_bai = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1.bam.bai" - purecn_ex1_interval = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1_intervals.txt" - purecn_ex1_normal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex1_normal.txt.gz" - purecn_ex2_normal = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_ex2_normal.txt.gz" - purecn_normalpanel_vcf = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_normalpanel.vcf.gz" - purecn_normalpanel_tbi = "${params.test_data_base}/data/genomics/homo_sapiens/illumina/purecn/purecn_normalpanel.vcf.gz.tbi" - } - 'gene_set_analysis' { - gct = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/P53_6samples_collapsed_symbols.gct" - cls = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/P53_6samples.cls" - gmx = "${params.test_data_base}/data/genomics/homo_sapiens/gene_set_analysis/c1.symbols.reduced.gmx" - } - 'cnvkit' { - amplicon_cnr = "https://raw.githubusercontent.com/etal/cnvkit/v0.9.9/test/formats/amplicon.cnr" - amplicon_cns = "https://raw.githubusercontent.com/etal/cnvkit/v0.9.9/test/formats/amplicon.cns" - } - } - 'generic' { - 'csv' { - test_csv = "${params.test_data_base}/data/generic/csv/test.csv" - } - 'notebooks' { - rmarkdown = "${params.test_data_base}/data/generic/notebooks/rmarkdown/rmarkdown_notebook.Rmd" - ipython_md = "${params.test_data_base}/data/generic/notebooks/jupyter/ipython_notebook.md" - ipython_ipynb = "${params.test_data_base}/data/generic/notebooks/jupyter/ipython_notebook.ipynb" - } - 'tar' { - tar_gz = "${params.test_data_base}/data/generic/tar/hello.tar.gz" - } - 'tsv' { - test_tsv = "${params.test_data_base}/data/generic/tsv/test.tsv" - } - 'txt' { - hello = "${params.test_data_base}/data/generic/txt/hello.txt" - } - 'unsorted_data' { - 'unsorted_text' { - genome_file = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.genome" - intervals = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.bed" - numbers_csv = "${params.test_data_base}/data/generic/unsorted_data/unsorted_text/test.csv" - } - } - } - } -} diff --git a/conf/test_full.config b/conf/test_full.config index bd109be..4320ea7 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -1,42 +1,36 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running full-size tests + Nextflow config file for running minimal tests ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Defines input files and everything required to run a full size pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_full, --outdir + nextflow run nf-core/variantbenchmarking -profile test_full, ---------------------------------------------------------------------------------------- */ params { - config_profile_name = 'Full test profile' - config_profile_description = 'Full test dataset to check pipeline function' + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' - // Input data for full size test - // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) - // TODO nf-core: Give any required params for the test so that command line flags are not needed + // Limit resources so that this can run on GitHub Actions + max_cpus = 16 + max_memory = 100.GB + max_time = '8.h' // Input data - sample = "HG002" - input = "assets/samplesheet_full.csv" + input = 'assets/samplesheet_full.csv' + outdir = 'results' // Genome references - genome = 'hg38' - - // Processes - analysis = 'germline' - method = 'happy,truvari,svanalyzer,rtgtools' // - preprocess = "normalization,deduplication" - min_sv_size = 30 - sv_standardization = "homogenize" - //variant_filtering = "include" // null, include, exclude - //expression = 'FILTER="."' - - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + genome = 'GRCh37' + analysis = 'germline' + sample = "HG002" + + method = 'truvari,svanalyzer,happy,rtgtools' + + preprocess = "normalization,deduplication,filter_contigs" + min_sv_size = 30 } diff --git a/conf/test_hg19.config b/conf/test_hg19.config deleted file mode 100644 index c7c2e18..0000000 --- a/conf/test_hg19.config +++ /dev/null @@ -1,41 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, --outdir - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = 100.GB - max_time = '8.h' - - // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'assets/samplesheet_HG002.csv' - outdir = 'results' - - // Genome references - genome = 'GRCh37' - analysis = 'germline' //somatic - method = 'truvari,svanalyzer' // --not working for now : wittyer, vcfdist - - standardization = true - preprocess = "normalization, deduplication" - //bnd_to_inv = true - - sample = "HG002" // available samples: SEQC2, HG002 - truth_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.vcf.gz" - high_conf_sv = "/Users/w620-admin/Desktop/nf-core/dataset/hg37/NIST_SV/HG002_SVs_Tier1_v0.6.bed" - -} diff --git a/conf/test_hg37.config b/conf/test_hg37.config deleted file mode 100644 index 5d21c30..0000000 --- a/conf/test_hg37.config +++ /dev/null @@ -1,44 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_hg37, --outdir - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = 100.GB - max_time = '8.h' - - // Input data - input = 'assets/samplesheet_HG002_hg37.csv' - outdir = 'results' - - // Genome references - genome = 'GRCh37' - analysis = 'germline' - sample = "HG002" - - method = 'truvari,svanalyzer' - - preprocess = "normalization,deduplication,filter_contigs" - min_sv_size = 30 - sv_standardization = "homogenize,svync" - - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" - truth_cnv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - high_conf_cnv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" - -} diff --git a/conf/test_hg37_full.config b/conf/test_hg37_full.config deleted file mode 100644 index 03c0d13..0000000 --- a/conf/test_hg37_full.config +++ /dev/null @@ -1,44 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/benchmark -profile -config conf/test_hg37_full.config --outdir - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = '100.GB' - max_time = '8.h' - - // Input data - input = 'assets/samplesheet_HG002_hg37_full.csv' - outdir = 'results' - - // Genome references - genome = 'GRCh37' - sample = "HG002" - - // Processes - analysis = 'germline' - method = 'truvari,svanalyzer,rtgtools,happy' // - preprocess = "normalization,deduplication,filter_contigs" - min_sv_size = 30 - //include_expression = 'FILTER="PASS"' - - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" - truth_cnv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - high_conf_cnv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" - -} diff --git a/conf/test_hg38.config b/conf/test_hg38.config deleted file mode 100644 index c0690db..0000000 --- a/conf/test_hg38.config +++ /dev/null @@ -1,44 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/benchmark -profile -config conf/test_hg38.config --outdir - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = '100.GB' - max_time = '8.h' - - // Input data - // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets - // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'assets/samplesheet_HG002_hg38.csv' - outdir = 'results' - - // Genome references - genome = 'GRCh38' - - // Processes - analysis = 'germline' //somatic - sample = "HG002" // available samples: SEQC2, HG002 - method = 'truvari,svanalyzer,rtgtools,happy' // - preprocess = "normalization,deduplication,filter_contigs" - min_sv_size = 30 - - //truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - //high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" - //truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - //high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" - //truth_cnv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - //high_conf_cnv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" -} diff --git a/conf/test_liftover.config b/conf/test_liftover.config index 9d668a7..4f19dc5 100644 --- a/conf/test_liftover.config +++ b/conf/test_liftover.config @@ -5,45 +5,40 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile -config conf/test_liftover.config --outdir + nextflow run nf-core/benchmark -profile test_liftover, --outdir ---------------------------------------------------------------------------------------- */ -// tests if liftover works, aim is to be use hg37 truth for hg38 variants or visa versa. -// liftover has to be turned on, chain, liftover genome and chain files must be provided - params { config_profile_name = 'Test profile' config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = '100.GB' + max_cpus = 2 + max_memory = '6.5GB' max_time = '8.h' // Input data - input = 'assets/samplesheet_HG002_hg38.csv' + input = 'assets/samplesheet.csv' outdir = 'results' - - // Genome references genome = 'GRCh38' // Processes - analysis = 'germline' //somatic - sample = "HG002" // available samples: SEQC2, HG002 - method = 'truvari,svanalyzer,rtgtools,happy' // + analysis = 'germline' + sample = "HG002" + method = 'truvari,svanalyzer,rtgtools,happy' preprocess = "normalization,deduplication,filter_contigs" min_sv_size = 30 - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" - itruth_ignore = true + truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" + high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" + truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" + high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" + itruth_ignore = true //liftover files - chain = "https://raw.githubusercontent.com/broadinstitute/gatk/master/scripts/funcotator/data_sources/gnomAD/b37ToHg38.over.chain" - rename_chr = "assets/rename_contigs/grch37_grch38.txt" - liftover = true + chain = "https://raw.githubusercontent.com/broadinstitute/gatk/master/scripts/funcotator/data_sources/gnomAD/b37ToHg38.over.chain" + rename_chr = "assets/rename_contigs/grch37_grch38.txt" + liftover = true } diff --git a/conf/test_small.config b/conf/test_small.config deleted file mode 100644 index c9a727f..0000000 --- a/conf/test_small.config +++ /dev/null @@ -1,38 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/variantbenchmarking -profile -config conf/test_small.config --outdir - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = '100.GB' - max_time = '8.h' - - // Input data - sample = "test" - input = "assets/samplesheet_small.csv" - - // Genome references - genome = 'hg38' - - // Processes - analysis = 'germline' - method = 'rtgtools,happy' // - preprocess = "normalization,deduplication,prepy" - //variant_filtering = "include" // null, include, exclude - //expression = 'FILTER="."' - - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" -} diff --git a/conf/test_hg38_somatic.config b/conf/test_somatic.config similarity index 86% rename from conf/test_hg38_somatic.config rename to conf/test_somatic.config index 3a18a42..345b68e 100644 --- a/conf/test_hg38_somatic.config +++ b/conf/test_somatic.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile -c conf/test_hg38_somatic.config --outdir + nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir ---------------------------------------------------------------------------------------- */ @@ -15,13 +15,13 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = '100.GB' + max_cpus = 2 + max_memory = '6.5GB' max_time = '8.h' // Input data - input = 'assets/samplesheet_SEQC2_sarek_test.csv' - outdir = 'results' + input = 'assets/samplesheet_somatic.csv' + outdir = 'results' // Genome references genome = 'GRCh38' diff --git a/conf/truth.config b/conf/truth.config index aad216a..cda4ac1 100644 --- a/conf/truth.config +++ b/conf/truth.config @@ -3,7 +3,7 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Config file for truth paths ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Can be used by any config that customises the base path using: + Can be used by any config that customizes the base path using: $params.truth / --truth ---------------------------------------------------------------------------------------- */ diff --git a/nextflow.config b/nextflow.config index a7fea87..e2babe0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -29,9 +29,6 @@ params { sv_standardization = "" // Filtering parameters - // minsize effects both truvari and variantbenchmarkingmark in different ways! svbechmark filters both base and comp calls - // although with truvari it is possible to filter only base, which makes more sense to me! - // I will use this parameter to filter SVs before benchmarking! min_sv_size = 30 max_sv_size = -1 min_allele_freq = -1 @@ -206,9 +203,9 @@ profiles { executor.memory = 8.GB } test { includeConfig 'conf/test.config' } - test_hg37 { includeConfig 'conf/test_hg37.config' } - test_hg38 { includeConfig 'conf/test_hg38.config' } - test_full { includeConfig 'conf/test_full.config' } + test_full { includeConfig 'conf/test_full.config' } + test_somatic { includeConfig 'conf/test_somatic.config' } + test_liftover { includeConfig 'conf/test_liftover.config' } } @@ -280,7 +277,7 @@ manifest { name = 'nf-core/variantbenchmarking' author = """kuebra.narci@dkfz.de""" homePage = 'https://github.com/nf-core/variantbenchmarking' - description = """Master benchmarking pipeline for Structural Variant callers """ + description = """Benchmarking pipeline for germline and somatic variant callers """ mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' version = '1.0dev' diff --git a/tests/main.nf.test b/tests/main.nf.test new file mode 100644 index 0000000..9014fad --- /dev/null +++ b/tests/main.nf.test @@ -0,0 +1,47 @@ +nextflow_pipeline { + + name "Test pipeline" + script "../main.nf" + tag "PIPELINE" + + test("Run with profile test") { + + when { + params { + input = 'assets/samplesheet_germline_hg38.csv' + sample = "HG002" + input = "assets/samplesheet_germline_hg38.csv" + outdir = "$outputDir" + genome = 'GRCh38' + analysis = 'germline' + method = 'happy,truvari,svanalyzer,wittyer,rtgtools' // + preprocess = "normalization,deduplication,prepy" + sv_standardization = "svync,homogenize" + include_expression = 'FILTER="."' + min_sv_size = 30 + truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" + truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + itruth_ignore = true + } + } + + then { + assert workflow.success + + assertAll( + { assert new File("$outputDir/samplesheet/samplesheet.csv").readLines().size() == 15 }, + { assert new File("$outputDir/samplesheet/samplesheet.csv").readLines()*.split(',')[0].take(4) == ['"sample"', '"fastq_1"', '"fastq_2"', '"run_accession"'] }, + { assert new File("$outputDir/samplesheet/samplesheet.csv").readLines()*.split(',').collect { it[0] } == ['"sample"', '"DRX024467"', '"DRX026011"', '"ERX1234253"', '"SRX10940790"', '"SRX11047067"', '"SRX17709227"', '"SRX17709228"', '"SRX6725035"', '"SRX9315476"', '"SRX9504942"', '"SRX9504942"', '"SRX9504942"', '"SRX9504942"', '"SRX9626017"'] }, + { assert new File("$outputDir/samplesheet/samplesheet.csv").text.contains('Illumina HiSeq 2500') }, + { assert new File("$outputDir/fastq/DRX024467_DRR026872.fastq.gz").exists() }, + { assert new File("$outputDir/fastq/SRX10940790_SRR14593545_1.fastq.gz").exists() }, + { assert new File("$outputDir/pipeline_info/nf_core_fetchngs_software_mqc_versions.yml").exists() }, + { assert new File("$outputDir/samplesheet/id_mappings.csv").exists() }, + { assert new File("$outputDir/samplesheet/multiqc_config.yml").exists() }, + { assert new File("$outputDir/samplesheet/samplesheet.csv").exists() } + ) + } + } +} diff --git a/tests/nextflow.config b/tests/nextflow.config new file mode 100644 index 0000000..d75ad44 --- /dev/null +++ b/tests/nextflow.config @@ -0,0 +1,27 @@ +params { + // Base directory for nf-core/modules test data + modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/' + + // Base directory for nf-core/fetchngs test data + pipelines_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/pipelines/fetchngs/1.15.0/' +} + +// Impose sensible resource limits for testing +process { + withName: '.*' { + cpus = 2 + memory = 3.GB + time = 2.h + } +} + +// Impose same minimum Nextflow version as the pipeline for testing +manifest { + nextflowVersion = '!>=23.04.0' +} + +// Disable all Nextflow reporting options +timeline { enabled = false } +report { enabled = false } +trace { enabled = false } +dag { enabled = false } diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 8dd6cf8..dfda72d 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -463,7 +463,6 @@ workflow VARIANTBENCHMARKING { ) ) - MULTIQC ( ch_multiqc_files.collect(), ch_multiqc_config.toList(), From 93abddbda6a2a6c69fea606623d98f483f8bb27e Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 16 Aug 2024 09:37:57 +0000 Subject: [PATCH 169/292] resources --- conf/test.config | 2 +- conf/test_full.config | 6 +++--- conf/test_liftover.config | 10 +++++----- 3 files changed, 9 insertions(+), 9 deletions(-) diff --git a/conf/test.config b/conf/test.config index bf6c831..7d3e247 100644 --- a/conf/test.config +++ b/conf/test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, + nextflow run nf-core/variantbenchmarking -profile test, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/test_full.config b/conf/test_full.config index 4320ea7..71ad921 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -15,9 +15,9 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on GitHub Actions - max_cpus = 16 - max_memory = 100.GB - max_time = '8.h' + max_cpus = 16 + max_memory = 100.GB + max_time = '8.h' // Input data input = 'assets/samplesheet_full.csv' diff --git a/conf/test_liftover.config b/conf/test_liftover.config index 4f19dc5..db3dee4 100644 --- a/conf/test_liftover.config +++ b/conf/test_liftover.config @@ -15,9 +15,9 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.5GB' - max_time = '8.h' + max_cpus = 2 + max_memory = '6.5GB' + max_time = '8.h' // Input data input = 'assets/samplesheet.csv' @@ -26,7 +26,7 @@ params { // Processes analysis = 'germline' - sample = "HG002" + sample = "HG002" method = 'truvari,svanalyzer,rtgtools,happy' preprocess = "normalization,deduplication,filter_contigs" min_sv_size = 30 @@ -34,7 +34,7 @@ params { truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" + high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" itruth_ignore = true //liftover files From e1c7286776ab2899489df645614fd581604dfae5 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 21 Aug 2024 10:24:08 +0000 Subject: [PATCH 170/292] stub update for modules --- conf/test_stub.config | 38 +++ modules.json | 6 +- modules/local/awk_sort.nf | 50 ---- .../evaluator/main.nf} | 0 .../local/bcftools/rename_chr/environment.yml | 7 + .../rename_chr/main.nf} | 0 modules/local/bgzip/tabix/environment.yml | 8 + .../{bgzip_tabix.nf => bgzip/tabix/main.nf} | 26 +- .../custom/reformat_header/environment.yml | 8 + .../reformat_header/main.nf} | 3 +- .../{sort_bed.nf => custom/sort_bed/main.nf} | 17 +- modules/local/gridss_annotation.nf | 47 ---- modules/local/merge_reports/main.nf | 1 - modules/local/plots/main.nf | 1 - modules/local/truvari_phab.nf | 43 --- modules/local/truvari_refine.nf | 39 --- modules/local/variant_extractor/main.nf | 3 +- modules/local/vcf_genotype_annotator.nf | 36 --- modules/local/vcf_to_csv/main.nf | 1 - .../local/visualize_tagged/environment.yml | 7 - modules/local/visualize_tagged/main.nf | 43 --- modules/nf-core/happy/happy/main.nf | 18 +- modules/nf-core/happy/happy/meta.yml | 31 +- .../nf-core/happy/happy/tests/main.nf.test | 96 +++++++ .../happy/happy/tests/main.nf.test.snap | 265 ++++++++++++++++++ .../picard/createsequencedictionary/main.nf | 12 + .../tests/main.nf.test | 61 ++++ .../tests/main.nf.test.snap | 48 ++++ modules/nf-core/ucsc/liftover/main.nf | 13 + modules/nf-core/ucsc/liftover/meta.yml | 6 +- .../nf-core/ucsc/liftover/tests/main.nf.test | 60 ++++ .../ucsc/liftover/tests/main.nf.test.snap | 69 +++++ modules/nf-core/ucsc/liftover/tests/tags.yml | 2 + .../nf-core/ucsc/liftover/tests/test.config | 5 + nextflow.config | 1 + .../local/compare_benchmark_results.nf | 2 +- subworkflows/local/liftover_vcfs_truth.nf | 7 +- subworkflows/local/small_somatic_benchmark.nf | 3 +- subworkflows/local/sv_somatic_benchmark.nf | 2 +- subworkflows/local/sv_vcf_conversion.nf | 2 +- 40 files changed, 776 insertions(+), 311 deletions(-) create mode 100644 conf/test_stub.config delete mode 100644 modules/local/awk_sort.nf rename modules/local/{bamsurgeon_evaluator.nf => bamsurgeon/evaluator/main.nf} (100%) create mode 100644 modules/local/bcftools/rename_chr/environment.yml rename modules/local/{bcftools_rename_chr.nf => bcftools/rename_chr/main.nf} (100%) create mode 100644 modules/local/bgzip/tabix/environment.yml rename modules/local/{bgzip_tabix.nf => bgzip/tabix/main.nf} (64%) create mode 100644 modules/local/custom/reformat_header/environment.yml rename modules/local/{reformat_header.nf => custom/reformat_header/main.nf} (92%) rename modules/local/{sort_bed.nf => custom/sort_bed/main.nf} (57%) delete mode 100644 modules/local/gridss_annotation.nf delete mode 100644 modules/local/truvari_phab.nf delete mode 100644 modules/local/truvari_refine.nf delete mode 100644 modules/local/vcf_genotype_annotator.nf delete mode 100644 modules/local/visualize_tagged/environment.yml delete mode 100644 modules/local/visualize_tagged/main.nf create mode 100644 modules/nf-core/happy/happy/tests/main.nf.test create mode 100644 modules/nf-core/happy/happy/tests/main.nf.test.snap create mode 100644 modules/nf-core/picard/createsequencedictionary/tests/main.nf.test create mode 100644 modules/nf-core/picard/createsequencedictionary/tests/main.nf.test.snap create mode 100644 modules/nf-core/ucsc/liftover/tests/main.nf.test create mode 100644 modules/nf-core/ucsc/liftover/tests/main.nf.test.snap create mode 100644 modules/nf-core/ucsc/liftover/tests/tags.yml create mode 100644 modules/nf-core/ucsc/liftover/tests/test.config diff --git a/conf/test_stub.config b/conf/test_stub.config new file mode 100644 index 0000000..9e9f4aa --- /dev/null +++ b/conf/test_stub.config @@ -0,0 +1,38 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test_stub -stub --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.5GB' + max_time = '8.h' + + // Input data + sample = "HG002" + input = "assets/samplesheet.csv" + outdir = "results" + + // Genome references + genome = 'GRCh38' + + // Processes + analysis = 'germline' + method = 'happy,truvari,svanalyzer,wittyer,rtgtools' // + preprocess = "normalization,deduplication,prepy" + sv_standardization = "svync,homogenize" + include_expression = 'FILTER="."' + min_sv_size = 30 + +} diff --git a/modules.json b/modules.json index ad80ae1..a5079cf 100644 --- a/modules.json +++ b/modules.json @@ -57,7 +57,7 @@ }, "happy/happy": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "41fc46dfd94dddf4fdee633629090c1c3bc9f668", "installed_by": ["modules"] }, "happy/prepy": { @@ -82,7 +82,7 @@ }, "picard/createsequencedictionary": { "branch": "master", - "git_sha": "0dee12b56ccecc416d6f7a1ccd85fd571eab3307", + "git_sha": "759232fe6aed8761bcb3a5d23813f2fa59d985ed", "installed_by": ["modules"] }, "picard/liftovervcf": { @@ -147,7 +147,7 @@ }, "ucsc/liftover": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "f67072004d8127beef877e7ba96ca5a05915d253", "installed_by": ["modules"] }, "wittyer": { diff --git a/modules/local/awk_sort.nf b/modules/local/awk_sort.nf deleted file mode 100644 index c115593..0000000 --- a/modules/local/awk_sort.nf +++ /dev/null @@ -1,50 +0,0 @@ -process AWK_SORT { - tag "$meta.id" - label 'process_low' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" - - input: - tuple val(meta),val(meta2), path(vcf) - - output: - tuple val(meta),val(meta2), path("*.tmp.vcf.gz"),path("*.tmp.vcf.gz.tbi"), emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def zipname = vcf.getBaseName() - - if (vcf.getExtension() == "gz"){ - """ - bgzip -d $vcf - cat $zipname | awk '\$1 ~ /^#/ {print \$0;next} {print \$0 | "sort -k1,1 -k2,2n"}' > ${zipname}.tmp.vcf - bgzip -c ${zipname}.tmp.vcf > ${zipname}.tmp.vcf.gz - tabix -p vcf ${zipname}.tmp.vcf.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') - END_VERSIONS - """ - } - else{ - """ - cat $vcf | awk '\$1 ~ /^#/ {print \$0;next} {print \$0 | "sort -k1,1 -k2,2n"}' > ${zipname}.tmp.vcf - bgzip -c ${zipname}.tmp.vcf > ${zipname}.tmp.vcf.gz - tabix -p vcf ${zipname}.tmp.vcf.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') - END_VERSIONS - """ - } -} diff --git a/modules/local/bamsurgeon_evaluator.nf b/modules/local/bamsurgeon/evaluator/main.nf similarity index 100% rename from modules/local/bamsurgeon_evaluator.nf rename to modules/local/bamsurgeon/evaluator/main.nf diff --git a/modules/local/bcftools/rename_chr/environment.yml b/modules/local/bcftools/rename_chr/environment.yml new file mode 100644 index 0000000..3d4e337 --- /dev/null +++ b/modules/local/bcftools/rename_chr/environment.yml @@ -0,0 +1,7 @@ +name: bcftools_annotate +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bcftools=1.20 diff --git a/modules/local/bcftools_rename_chr.nf b/modules/local/bcftools/rename_chr/main.nf similarity index 100% rename from modules/local/bcftools_rename_chr.nf rename to modules/local/bcftools/rename_chr/main.nf diff --git a/modules/local/bgzip/tabix/environment.yml b/modules/local/bgzip/tabix/environment.yml new file mode 100644 index 0000000..bd52fad --- /dev/null +++ b/modules/local/bgzip/tabix/environment.yml @@ -0,0 +1,8 @@ +name: bgzip_tabix +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/local/bgzip_tabix.nf b/modules/local/bgzip/tabix/main.nf similarity index 64% rename from modules/local/bgzip_tabix.nf rename to modules/local/bgzip/tabix/main.nf index 986eae4..f1b7f38 100644 --- a/modules/local/bgzip_tabix.nf +++ b/modules/local/bgzip/tabix/main.nf @@ -2,7 +2,7 @@ process BGZIP_TABIX { tag "$meta.id" label 'process_single' - conda "" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : 'biocontainers/tabix:1.11--hdfd78af_0' }" @@ -19,13 +19,12 @@ process BGZIP_TABIX { script: def args = task.ext.args ?: '' - def args2 = task.ext.args2 ?: '' - def zipname = vcf.getBaseName() + ".temp.vcf.gz" + def prefix = task.ext.prefix ?: "${meta.id}" if (vcf.getExtension() == "gz"){ """ - cp $vcf $zipname - tabix -p vcf $zipname + cp $vcf ${prefix}.vcf.gz + tabix -p vcf ${prefix}.vcf.gz cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -35,8 +34,8 @@ process BGZIP_TABIX { } else{ """ - bgzip ${args2} --threads ${task.cpus} -c $vcf > $zipname - tabix -p vcf $zipname + bgzip ${args} --threads ${task.cpus} -c $vcf > ${prefix}.vcf.gz + tabix -p vcf ${prefix}.vcf.gz cat <<-END_VERSIONS > versions.yml "${task.process}": @@ -44,4 +43,17 @@ process BGZIP_TABIX { END_VERSIONS """ } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/local/custom/reformat_header/environment.yml b/modules/local/custom/reformat_header/environment.yml new file mode 100644 index 0000000..361c078 --- /dev/null +++ b/modules/local/custom/reformat_header/environment.yml @@ -0,0 +1,8 @@ +name: tabix_bgzip +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/local/reformat_header.nf b/modules/local/custom/reformat_header/main.nf similarity index 92% rename from modules/local/reformat_header.nf rename to modules/local/custom/reformat_header/main.nf index 58b33ac..c1de997 100644 --- a/modules/local/reformat_header.nf +++ b/modules/local/custom/reformat_header/main.nf @@ -36,7 +36,6 @@ process REFORMAT_HEADER { END_VERSIONS """ stub: - def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}.reformatted.vcf.gz @@ -44,7 +43,7 @@ process REFORMAT_HEADER { cat <<-END_VERSIONS > versions.yml "${task.process}": - r-base: \$(echo \$(R --version 2>&1) | sed 's/^.*R version //; s/ .*\$//') + tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') END_VERSIONS """ } diff --git a/modules/local/sort_bed.nf b/modules/local/custom/sort_bed/main.nf similarity index 57% rename from modules/local/sort_bed.nf rename to modules/local/custom/sort_bed/main.nf index cca5e72..58e3a18 100644 --- a/modules/local/sort_bed.nf +++ b/modules/local/custom/sort_bed/main.nf @@ -2,17 +2,16 @@ process SORT_BED { tag "$meta.id" label 'process_low' - conda "${moduleDir}/environment.yml" + conda "conda-forge::tar=1.34" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.17--haef29d1_0': - 'quay.io/biocontainers/bcftools:1.17--haef29d1_0' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'quay.io/nf-core/ubuntu:20.04' }" input: tuple val(meta), path(bed) output: tuple val(meta), path("*.bed"), emit: bed - path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -24,12 +23,12 @@ process SORT_BED { // sorts the positions by -k1,1 -k2,2n """ sort -k1,1 -k2,2n $bed > ${prefix}.bed - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - tabix: \$(echo \$(tabix -h 2>&1) | sed 's/^.*Version: //; s/ .*\$//') - END_VERSIONS """ + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bed + """ } diff --git a/modules/local/gridss_annotation.nf b/modules/local/gridss_annotation.nf deleted file mode 100644 index 7be0a5b..0000000 --- a/modules/local/gridss_annotation.nf +++ /dev/null @@ -1,47 +0,0 @@ -// UNTESTED -process GRIDSS_ANNOTATION { - tag "$meta.id" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gridss:2.13.2--h270b39a_0': - 'biocontainers/gridss:2.13.2--h270b39a_0' }" - - input: - tuple val(meta), path(vcf), path(index) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fasta_fai) - - output: - tuple val(meta),path("*.vcf.gz"),path("*.vcf.gz.tbi") , emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def genome = params.genome.contains("38") ? "hg38": "hg19" - def VERSION = '2.13.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. - - """ - bgzip -d $vcf -c > unzziped.vcf - simple_event-annotator.R \\ - unzziped.vcf \\ - ${prefix}.vcf \\ - ${genome} - - bgzip --threads ${task.cpus} -c ${prefix}.vcf > ${prefix}.anno.vcf.gz - tabix -p vcf ${prefix}.anno.vcf.gz - - rm unzziped.vcf - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - gridss: ${VERSION} - END_VERSIONS - """ - -} diff --git a/modules/local/merge_reports/main.nf b/modules/local/merge_reports/main.nf index e62872a..70d7d06 100644 --- a/modules/local/merge_reports/main.nf +++ b/modules/local/merge_reports/main.nf @@ -34,7 +34,6 @@ process MERGE_REPORTS { END_VERSIONS """ stub: - def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.benchmark_tool}" """ touch ${prefix}.summary.csv diff --git a/modules/local/plots/main.nf b/modules/local/plots/main.nf index 751cf39..ed8da3a 100644 --- a/modules/local/plots/main.nf +++ b/modules/local/plots/main.nf @@ -30,7 +30,6 @@ process PLOTS { END_VERSIONS """ stub: - def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.benchmark_tool}" """ touch metric_by_tool_${prefix}.png diff --git a/modules/local/truvari_phab.nf b/modules/local/truvari_phab.nf deleted file mode 100644 index 0f032ff..0000000 --- a/modules/local/truvari_phab.nf +++ /dev/null @@ -1,43 +0,0 @@ -process TRUVARI_PHAB { - tag "$meta.id $meta2.caller" - label 'process_medium' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker://kubran/truvari:v4.3.0': - 'kubran/truvari:v4.3.0' }" - - input: - tuple val(meta), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi), path(bed) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - - output: - tuple val(meta),path("*.vcf.gz") , emit: harmon - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def regions = bed ? "--region $bed" : "" - - """ - truvari phab \\ - --base ${truth_vcf} \\ - --comp ${vcf} \\ - --bSample $meta.id \\ - --cSample $meta.id \\ - --reference ${fasta} \\ - --output ${prefix}.vcf.gz \\ - ${regions} \\ - ${args} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - truvari: \$(echo \$(truvari version 2>&1) | sed 's/^Truvari v//' )) - END_VERSIONS - """ -} diff --git a/modules/local/truvari_refine.nf b/modules/local/truvari_refine.nf deleted file mode 100644 index a3748f0..0000000 --- a/modules/local/truvari_refine.nf +++ /dev/null @@ -1,39 +0,0 @@ -process TRUVARI_REFINE { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker://kubran/truvari:v4.3.0': - 'kubran/truvari:v4.3.0' }" - - input: - tuple val(meta), path(bench) - each path(bed) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - - output: - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - - """ - truvari refine \\ - --use-original-vcfs \\ - --reference $fasta \\ - --regions $bed \\ - $bench - . - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - truvari: \$(echo \$(truvari version 2>&1) | sed 's/^Truvari v//' )) - END_VERSIONS - """ -} diff --git a/modules/local/variant_extractor/main.nf b/modules/local/variant_extractor/main.nf index e2eb5e3..b2bb932 100644 --- a/modules/local/variant_extractor/main.nf +++ b/modules/local/variant_extractor/main.nf @@ -36,10 +36,9 @@ process VARIANT_EXTRACTOR { END_VERSIONS """ stub: - def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ - touch ${prefix}.norm.vcf + touch ${prefix}.norm.vcf.gz cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/local/vcf_genotype_annotator.nf b/modules/local/vcf_genotype_annotator.nf deleted file mode 100644 index 1931b8a..0000000 --- a/modules/local/vcf_genotype_annotator.nf +++ /dev/null @@ -1,36 +0,0 @@ -process VCF_GENOTYPE_ANNOTATOR { - tag "$meta.id" - label 'process_low' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker://griffithlab/vatools:5.1.10' - 'griffithlab/vatools:5.1.10' }" - - input: - tuple val(meta),path(vcf) - - output: - tuple val(meta),path("*.{vcf}"), emit: vcf - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - - """ - vcf-genotype-annotator \\ - $vcf \\ - ${meta.id} \\ - $args - -o ${prefix}.vcf - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) - END_VERSIONS - """ -} diff --git a/modules/local/vcf_to_csv/main.nf b/modules/local/vcf_to_csv/main.nf index bff1b21..f17e7a4 100644 --- a/modules/local/vcf_to_csv/main.nf +++ b/modules/local/vcf_to_csv/main.nf @@ -31,7 +31,6 @@ process VCF_TO_CSV { END_VERSIONS """ stub: - def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}.csv diff --git a/modules/local/visualize_tagged/environment.yml b/modules/local/visualize_tagged/environment.yml deleted file mode 100644 index 6d60d87..0000000 --- a/modules/local/visualize_tagged/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: plots -channels: - - conda-forge -dependencies: - - r-base=4.1.* - - r-ggplot2=3.3.* - - r-reshape2=1.4.* diff --git a/modules/local/visualize_tagged/main.nf b/modules/local/visualize_tagged/main.nf deleted file mode 100644 index 2f4ee02..0000000 --- a/modules/local/visualize_tagged/main.nf +++ /dev/null @@ -1,43 +0,0 @@ -process VISUALIZE_TAGGED { - tag "$meta.benchmark_tool" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/r-shinyngs:1.8.4--r43hdfd78af_0': - 'biocontainers/r-shinyngs:1.8.4--r43hdfd78af_0' }" - - input: - tuple val(meta), path(vcf) - - output: - tuple val(meta),path("*.txt"), emit: plots - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.benchmark_tool}" - - """ - grep -o 'SUPP_VEC=[^,;]*' $vcf | awk -F'=' '{print \$2}' | sed -e 's/\\(.\\)/\\1 /g' > ${vcf}.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - r-base: \$(echo \$(R --version 2>&1) | sed 's/^.*R version //; s/ .*\$//') - END_VERSIONS - """ - stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.benchmark_tool}" - """ - touch ${vcf}.txt - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - r-base: \$(echo \$(R --version 2>&1) | sed 's/^.*R version //; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/happy/happy/main.nf b/modules/nf-core/happy/happy/main.nf index b3cc12d..a5757db 100644 --- a/modules/nf-core/happy/happy/main.nf +++ b/modules/nf-core/happy/happy/main.nf @@ -62,19 +62,21 @@ process HAPPY_HAPPY { stub: def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" def VERSION = '0.3.14' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ + echo "" | gzip > ${prefix}.roc.all.csv.gz + echo "" | gzip > ${prefix}.roc.Locations.INDEL.csv.gz + echo "" | gzip > ${prefix}.roc.Locations.INDEL.PASS.csv.gz + echo "" | gzip > ${prefix}.roc.Locations.SNP.csv.gz + echo "" | gzip > ${prefix}.roc.Locations.SNP.PASS.csv.gz + echo "" | gzip > ${prefix}.metrics.json.gz + echo "" | gzip > ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi touch ${prefix}.summary.csv - touch ${prefix}.roc.all.csv.gz - touch ${prefix}.roc.Locations.INDEL.csv.gz - touch ${prefix}.roc.Locations.INDEL.PASS.csv.gz - touch ${prefix}.roc.Locations.SNP.csv.gz - touch ${prefix}.roc.Locations.SNP.PASS.csv.gz touch ${prefix}.extended.csv touch ${prefix}.runinfo.json - touch ${prefix}.metrics.json.gz - touch ${prefix}.vcf.gz - touch ${prefix}.vcf.gz.tbi + cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/happy/happy/meta.yml b/modules/nf-core/happy/happy/meta.yml index 85912cb..dc8ebf2 100644 --- a/modules/nf-core/happy/happy/meta.yml +++ b/modules/nf-core/happy/happy/meta.yml @@ -11,13 +11,38 @@ tools: homepage: "https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub/apps/hap-py-benchmarking.html" documentation: "https://github.com/Illumina/hap.py" tool_dev_url: "https://github.com/Illumina/hap.py" - licence: "['BSD-2-clause']" + licence: ["BSD-2-clause"] input: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] + - meta2: + type: map + description: | + Groovy Map containing fasta file information + e.g. [ id:'test2'] + - meta3: + type: map + description: | + Groovy Map containing fai file information + e.g. [ id:'test3'] + - meta4: + type: map + description: | + Groovy Map containing false_positives_bed file information + e.g. [ id:'test4'] + - meta5: + type: map + description: | + Groovy Map containing stratification_tsv file information + e.g. [ id:'test5'] + - meta6: + type: map + description: | + Groovy Map containing stratification_beds file information + e.g. [ id:'test6'] - query_vcf: type: file description: VCF/GVCF file to query @@ -51,7 +76,7 @@ input: description: Stratification file list in TSV format pattern: "*.tsv" - stratification_beds: - type: file(s) + type: file description: One or more BED files used for stratification (these should be referenced in the stratification TSV) pattern: "*.bed" output: @@ -88,7 +113,7 @@ output: type: file description: A CSV file containing extended info of the benchmarking pattern: "*.extended.csv" - - json: + - metrics_json: type: file description: A JSON file containing the run info pattern: "*.runinfo.json" diff --git a/modules/nf-core/happy/happy/tests/main.nf.test b/modules/nf-core/happy/happy/tests/main.nf.test new file mode 100644 index 0000000..a824297 --- /dev/null +++ b/modules/nf-core/happy/happy/tests/main.nf.test @@ -0,0 +1,96 @@ +nextflow_process { + + name "Test Process HAPPY_HAPPY" + script "../main.nf" + process "HAPPY_HAPPY" + + tag "modules" + tag "modules_nfcore" + tag "happy" + tag "happy/happy" + + test("homo_sapiens - illumina - vcf") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true) + ] + input[3] = [[],[]] + input[4] = [[],[]] + input[5] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.vcf.get(0).get(1)).linesGzip.contains("##fileformat=VCFv4.1") }, + { assert path(process.out.roc_all_csv.get(0).get(1)).linesGzip.toString().contains("Type") }, + { assert path(process.out.roc_indel_locations_csv.get(0).get(1)).linesGzip.toString().contains("Type") }, + { assert path(process.out.roc_snp_locations_csv.get(0).get(1)).linesGzip.toString().contains("Type") }, + { assert path(process.out.roc_indel_locations_pass_csv.get(0).get(1)).linesGzip.toString().contains("Type") }, + { assert path(process.out.roc_snp_locations_pass_csv.get(0).get(1)).linesGzip.toString().contains("Type") }, + { assert snapshot(process.out.summary_csv, + process.out.extended_csv, + process.out.versions ).match() }, + { assert snapshot(file(process.out.metrics_json[0][1]).name).match("metrics_json_1")}, + { assert snapshot(file(process.out.runinfo[0][1]).name).match("runinfo_1")}, + { assert snapshot(file(process.out.tbi[0][1]).name).match("tbi_1")}, + { assert snapshot(process.out.versions ).match("versions_1") } + ) + } + } + test("homo_sapiens - illumina - vcf - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true) + ] + input[3] = [[],[]] + input[4] = [[],[]] + input[5] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/happy/happy/tests/main.nf.test.snap b/modules/nf-core/happy/happy/tests/main.nf.test.snap new file mode 100644 index 0000000..5c69e7f --- /dev/null +++ b/modules/nf-core/happy/happy/tests/main.nf.test.snap @@ -0,0 +1,265 @@ +{ + "runinfo_1": { + "content": [ + "test.runinfo.json" + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-19T14:11:48.042647133" + }, + "homo_sapiens - illumina - vcf": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.summary.csv:md5,647acf226e2545624a8e71812a984ea7" + ] + ], + [ + [ + { + "id": "test" + }, + "test.extended.csv:md5,4b53c835cd20fe0b14fcb4b2ef6b0f52" + ] + ], + [ + "versions.yml:md5,4950956e15868125751a19333eae8743" + ] + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-19T14:11:48.021071904" + }, + "versions_1": { + "content": [ + [ + "versions.yml:md5,4950956e15868125751a19333eae8743" + ] + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-20T08:15:53.839842249" + }, + "metrics_json_1": { + "content": [ + "test.metrics.json.gz" + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-19T14:05:27.732904018" + }, + "homo_sapiens - illumina - vcf - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.roc.all.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "10": [ + [ + { + "id": "test" + }, + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "11": [ + "versions.yml:md5,4950956e15868125751a19333eae8743" + ], + "2": [ + [ + { + "id": "test" + }, + "test.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "3": [ + [ + { + "id": "test" + }, + "test.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "4": [ + [ + { + "id": "test" + }, + "test.roc.Locations.SNP.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "5": [ + [ + { + "id": "test" + }, + "test.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "6": [ + [ + { + "id": "test" + }, + "test.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "7": [ + [ + { + "id": "test" + }, + "test.runinfo.json:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "8": [ + [ + { + "id": "test" + }, + "test.metrics.json.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "9": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "extended_csv": [ + [ + { + "id": "test" + }, + "test.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "metrics_json": [ + [ + { + "id": "test" + }, + "test.metrics.json.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "roc_all_csv": [ + [ + { + "id": "test" + }, + "test.roc.all.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "roc_indel_locations_csv": [ + [ + { + "id": "test" + }, + "test.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "roc_indel_locations_pass_csv": [ + [ + { + "id": "test" + }, + "test.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "roc_snp_locations_csv": [ + [ + { + "id": "test" + }, + "test.roc.Locations.SNP.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "roc_snp_locations_pass_csv": [ + [ + { + "id": "test" + }, + "test.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "runinfo": [ + [ + { + "id": "test" + }, + "test.runinfo.json:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "summary_csv": [ + [ + { + "id": "test" + }, + "test.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + [ + { + "id": "test" + }, + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,4950956e15868125751a19333eae8743" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-19T13:23:08.786848304" + }, + "tbi_1": { + "content": [ + "test.vcf.gz.tbi" + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-19T14:11:48.081967471" + } +} \ No newline at end of file diff --git a/modules/nf-core/picard/createsequencedictionary/main.nf b/modules/nf-core/picard/createsequencedictionary/main.nf index bc925a0..4630a0d 100644 --- a/modules/nf-core/picard/createsequencedictionary/main.nf +++ b/modules/nf-core/picard/createsequencedictionary/main.nf @@ -39,4 +39,16 @@ process PICARD_CREATESEQUENCEDICTIONARY { picard: \$(picard CreateSequenceDictionary --version 2>&1 | grep -o 'Version:.*' | cut -f2- -d:) END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.dict + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + picard: \$(echo \$(picard CreateSequenceDictionary --version 2>&1) | grep -o 'Version:.*' | cut -f2- -d:) + END_VERSIONS + """ + } diff --git a/modules/nf-core/picard/createsequencedictionary/tests/main.nf.test b/modules/nf-core/picard/createsequencedictionary/tests/main.nf.test new file mode 100644 index 0000000..8152be9 --- /dev/null +++ b/modules/nf-core/picard/createsequencedictionary/tests/main.nf.test @@ -0,0 +1,61 @@ +nextflow_process { + + name "Test Process PICARD_CREATESEQUENCEDICTIONARY" + script "../main.nf" + process "PICARD_CREATESEQUENCEDICTIONARY" + + tag "modules" + tag "modules_nfcore" + tag "picard" + tag "picard/createsequencedictionary" + + test("sarscov2 - fasta") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + file(process.out.reference_dict[0][1]).name, + process.out.versions + ).match() + } + + ) + } + + } + + test("sarscov2 - fasta - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true)] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/picard/createsequencedictionary/tests/main.nf.test.snap b/modules/nf-core/picard/createsequencedictionary/tests/main.nf.test.snap new file mode 100644 index 0000000..3f3f8c8 --- /dev/null +++ b/modules/nf-core/picard/createsequencedictionary/tests/main.nf.test.snap @@ -0,0 +1,48 @@ +{ + "sarscov2 - fasta - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.dict:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,fdb110d8c303ffa0615bb200f9a2c999" + ], + "reference_dict": [ + [ + { + "id": "test" + }, + "test.dict:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,fdb110d8c303ffa0615bb200f9a2c999" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-19T07:02:59.686224426" + }, + "sarscov2 - fasta": { + "content": [ + "test.dict", + [ + "versions.yml:md5,fdb110d8c303ffa0615bb200f9a2c999" + ] + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-16T14:31:34.330709472" + } +} \ No newline at end of file diff --git a/modules/nf-core/ucsc/liftover/main.nf b/modules/nf-core/ucsc/liftover/main.nf index d9725ba..733c8dd 100644 --- a/modules/nf-core/ucsc/liftover/main.nf +++ b/modules/nf-core/ucsc/liftover/main.nf @@ -37,4 +37,17 @@ process UCSC_LIFTOVER { ucsc: $VERSION END_VERSIONS """ + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '377' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + touch ${prefix}.lifted.bed + touch ${prefix}.unlifted.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + ucsc: $VERSION + END_VERSIONS + """ + } diff --git a/modules/nf-core/ucsc/liftover/meta.yml b/modules/nf-core/ucsc/liftover/meta.yml index 34a4e06..c1c40d9 100644 --- a/modules/nf-core/ucsc/liftover/meta.yml +++ b/modules/nf-core/ucsc/liftover/meta.yml @@ -19,13 +19,17 @@ input: type: file description: Browser Extensible Data (BED) file pattern: "*.{bed}" + - chain: + type: file + description: Chain file + pattern: "*.{chain,chain.gz}" output: - meta: type: map description: | Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - - version: + - versions: type: file description: File containing software version pattern: "versions.yml" diff --git a/modules/nf-core/ucsc/liftover/tests/main.nf.test b/modules/nf-core/ucsc/liftover/tests/main.nf.test new file mode 100644 index 0000000..8aee1ee --- /dev/null +++ b/modules/nf-core/ucsc/liftover/tests/main.nf.test @@ -0,0 +1,60 @@ +nextflow_process { + + name "Test Process UCSC_LIFTOVER" + script "../main.nf" + process "UCSC_LIFTOVER" + tag "modules" + tag "modules_nfcore" + tag "ucsc" + tag "ucsc/liftover" + config "./test.config" + + test("genome - bed") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.bed", checkIfExists: true) + ]) + input[1] = Channel.of(file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.chain.gz", checkIfExists: true)) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.lifted, + file(process.out.unlifted[0][1]).name + ).match() }, + + ) + } + } + + test("genome - bed - stub") { + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.bed", checkIfExists: true) + ]) + input[1] = Channel.of(file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/genome.chain.gz", checkIfExists: true)) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} \ No newline at end of file diff --git a/modules/nf-core/ucsc/liftover/tests/main.nf.test.snap b/modules/nf-core/ucsc/liftover/tests/main.nf.test.snap new file mode 100644 index 0000000..af32008 --- /dev/null +++ b/modules/nf-core/ucsc/liftover/tests/main.nf.test.snap @@ -0,0 +1,69 @@ +{ + "genome - bed": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.lifted.bed:md5,fd5878470257a8a0edeaa8b9374bd520" + ] + ], + "test.unlifted.bed" + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-16T14:17:55.287355152" + }, + "genome - bed - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.lifted.bed:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.unlifted.bed:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,cad46ffe44c53a79ebab54d7047fb674" + ], + "lifted": [ + [ + { + "id": "test" + }, + "test.lifted.bed:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "unlifted": [ + [ + { + "id": "test" + }, + "test.unlifted.bed:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,cad46ffe44c53a79ebab54d7047fb674" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-16T12:48:23.999969072" + } +} \ No newline at end of file diff --git a/modules/nf-core/ucsc/liftover/tests/tags.yml b/modules/nf-core/ucsc/liftover/tests/tags.yml new file mode 100644 index 0000000..1ba221d --- /dev/null +++ b/modules/nf-core/ucsc/liftover/tests/tags.yml @@ -0,0 +1,2 @@ +ucsc/liftover: + - modules/nf-core/ucsc/liftover/** diff --git a/modules/nf-core/ucsc/liftover/tests/test.config b/modules/nf-core/ucsc/liftover/tests/test.config new file mode 100644 index 0000000..b261ec6 --- /dev/null +++ b/modules/nf-core/ucsc/liftover/tests/test.config @@ -0,0 +1,5 @@ +process { + withName: UCSC_LIFTOVER { + ext.prefix = "test" + } +} diff --git a/nextflow.config b/nextflow.config index e2babe0..09c9021 100644 --- a/nextflow.config +++ b/nextflow.config @@ -203,6 +203,7 @@ profiles { executor.memory = 8.GB } test { includeConfig 'conf/test.config' } + test_stub { includeConfig 'conf/test_stub.config' } test_full { includeConfig 'conf/test_full.config' } test_somatic { includeConfig 'conf/test_somatic.config' } test_liftover { includeConfig 'conf/test_liftover.config' } diff --git a/subworkflows/local/compare_benchmark_results.nf b/subworkflows/local/compare_benchmark_results.nf index e04c91d..5fdae49 100644 --- a/subworkflows/local/compare_benchmark_results.nf +++ b/subworkflows/local/compare_benchmark_results.nf @@ -7,7 +7,7 @@ include { SURVIVOR_MERGE } from '../../modules/nf-core/survivor/merge' include { BCFTOOLS_MERGE } from '../../modules/nf-core/bcftools/merge' include { VCF_TO_CSV } from '../../modules/local/vcf_to_csv' include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' -include { REFORMAT_HEADER } from '../../modules/local/reformat_header' +include { REFORMAT_HEADER } from '../../modules/local/custom/reformat_header' workflow COMPARE_BENCHMARK_RESULTS { take: diff --git a/subworkflows/local/liftover_vcfs_truth.nf b/subworkflows/local/liftover_vcfs_truth.nf index c6e030d..c955bb1 100644 --- a/subworkflows/local/liftover_vcfs_truth.nf +++ b/subworkflows/local/liftover_vcfs_truth.nf @@ -4,10 +4,10 @@ include { PICARD_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/picard/createsequencedictionary' include { PICARD_LIFTOVERVCF } from '../../modules/nf-core/picard/liftovervcf' -include { REFORMAT_HEADER } from '../../modules/local/reformat_header.nf' -include { BCFTOOLS_RENAME_CHR } from '../../modules/local/bcftools_rename_chr.nf' +include { REFORMAT_HEADER } from '../../modules/local/custom/reformat_header' +include { BCFTOOLS_RENAME_CHR } from '../../modules/local/bcftools/rename_chr' include { UCSC_LIFTOVER } from '../../modules/nf-core/ucsc/liftover' -include { SORT_BED } from '../../modules/local/sort_bed.nf' +include { SORT_BED } from '../../modules/local/custom/sort_bed' include { BEDTOOLS_MERGE } from '../../modules/nf-core/bedtools/merge' @@ -67,7 +67,6 @@ workflow LIFTOVER_VCFS_TRUTH { SORT_BED( UCSC_LIFTOVER.out.lifted ) - versions = versions.mix(SORT_BED.out.versions.first()) // merge the intersected regions BEDTOOLS_MERGE( diff --git a/subworkflows/local/small_somatic_benchmark.nf b/subworkflows/local/small_somatic_benchmark.nf index 4546828..cfd2e99 100644 --- a/subworkflows/local/small_somatic_benchmark.nf +++ b/subworkflows/local/small_somatic_benchmark.nf @@ -3,7 +3,7 @@ // include { HAPPY_SOMPY } from '../../modules/nf-core/happy/sompy/main' -include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon_evaluator.nf' +include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon/evaluator' workflow SMALL_SOMATIC_BENCHMARK { take: @@ -37,6 +37,7 @@ workflow SMALL_SOMATIC_BENCHMARK { summary_reports = summary_reports.mix(report) } + // not working for now if (params.method.contains('bamsurgeon')){ BAMSURGEON_EVALUATOR( diff --git a/subworkflows/local/sv_somatic_benchmark.nf b/subworkflows/local/sv_somatic_benchmark.nf index 44f43a9..b9b31ab 100644 --- a/subworkflows/local/sv_somatic_benchmark.nf +++ b/subworkflows/local/sv_somatic_benchmark.nf @@ -2,7 +2,7 @@ // SOMATIC: SUBWORKFLOW FOR SMALL SOMATIC VARIANTS // -include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon_evaluator.nf' +include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon/evaluator' workflow SV_SOMATIC_BENCHMARK { take: diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 16c914d..ec3717d 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -5,7 +5,7 @@ import groovy.io.FileType // include { SVYNC } from '../../modules/nf-core/svync' -include { BGZIP_TABIX } from '../../modules/local/bgzip_tabix' +include { BGZIP_TABIX } from '../../modules/local/bgzip/tabix' include { VARIANT_EXTRACTOR } from '../../modules/local/variant_extractor' include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' From 07c17373c270077c4f2a48201fd635387261fabe Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 21 Aug 2024 10:30:46 +0000 Subject: [PATCH 171/292] add everything to stub --- conf/test_stub.config | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/conf/test_stub.config b/conf/test_stub.config index 9e9f4aa..fdbf8b8 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -21,7 +21,7 @@ params { // Input data sample = "HG002" - input = "assets/samplesheet.csv" + input = "assets/samplesheet_somatic.csv" outdir = "results" // Genome references @@ -34,5 +34,6 @@ params { sv_standardization = "svync,homogenize" include_expression = 'FILTER="."' min_sv_size = 30 + liftover = true } From 400096e5e377b9a293a4d4985b246d519f5e0858 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 21 Aug 2024 10:54:33 +0000 Subject: [PATCH 172/292] add nf-test --- .gitignore | 1 + .nf-test.log | 54 +++++++++++++++++++++++++++++++++++++++++++ tests/main.nf.test | 19 ++++----------- tests/nextflow.config | 27 ---------------------- 4 files changed, 60 insertions(+), 41 deletions(-) create mode 100644 .nf-test.log delete mode 100644 tests/nextflow.config diff --git a/.gitignore b/.gitignore index 8f071fd..8d2b4f7 100644 --- a/.gitignore +++ b/.gitignore @@ -11,4 +11,5 @@ test_sv*/ test/ null/* results*/* +.nf-test/* diff --git a/.nf-test.log b/.nf-test.log new file mode 100644 index 0000000..e04c614 --- /dev/null +++ b/.nf-test.log @@ -0,0 +1,54 @@ +Aug-21 10:47:08.575 [main] INFO com.askimed.nf.test.App - nf-test 0.8.4 +Aug-21 10:47:08.597 [main] INFO com.askimed.nf.test.App - Arguments: [test, tests/main.nf.test, --profile, docker] +Aug-21 10:47:09.754 [main] INFO com.askimed.nf.test.App - Nextflow Version: 24.04.4 +Aug-21 10:47:09.758 [main] WARN com.askimed.nf.test.commands.RunTestsCommand - No nf-test config file found. +Aug-21 10:47:09.759 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Detected 1 test files. +Aug-21 10:47:10.462 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/workspace/variantbenchmarking/bin' to '/workspace/variantbenchmarking/.nf-test/tests/bf2b50ea975fb31273e738e1ac12ece5/meta/bin' +Aug-21 10:47:10.468 [main] WARN com.askimed.nf.test.config.FileStaging - File '/workspace/variantbenchmarking/lib' not found. Ignore it. +Aug-21 10:47:10.468 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/workspace/variantbenchmarking/assets' to '/workspace/variantbenchmarking/.nf-test/tests/bf2b50ea975fb31273e738e1ac12ece5/meta/assets' +Aug-21 10:47:10.482 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Started test plan +Aug-21 10:47:10.483 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Running testsuite 'Test pipeline' from file '/workspace/variantbenchmarking/tests/main.nf.test'. +Aug-21 10:47:10.483 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Run test 'bf2b50ea: Run with profile test'. type: com.askimed.nf.test.lang.pipeline.PipelineTest +Aug-21 10:50:58.838 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Test 'bf2b50ea: Run with profile test' finished. status: FAILED +org.codehaus.groovy.runtime.powerassert.PowerAssertionError: 1 of 1 assertions failed + at com.askimed.nf.test.lang.extensions.GlobalMethods.assertAll(GlobalMethods.java:48) + at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke0(Native Method) + at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:77) + at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) + at java.base/java.lang.reflect.Method.invoke(Method.java:568) + at org.codehaus.groovy.reflection.CachedMethod.invoke(CachedMethod.java:107) + at groovy.lang.MetaMethod.doMethodInvoke(MetaMethod.java:323) + at org.codehaus.groovy.runtime.callsite.StaticMetaMethodSite.invoke(StaticMetaMethodSite.java:44) + at org.codehaus.groovy.runtime.callsite.StaticMetaMethodSite.callStatic(StaticMetaMethodSite.java:100) + at org.codehaus.groovy.runtime.callsite.CallSiteArray.defaultCallStatic(CallSiteArray.java:55) + at org.codehaus.groovy.runtime.callsite.AbstractCallSite.callStatic(AbstractCallSite.java:217) + at org.codehaus.groovy.runtime.callsite.AbstractCallSite.callStatic(AbstractCallSite.java:231) + at main_nf$_run_closure1$_closure2$_closure4.doCall(main.nf.test:33) + at main_nf$_run_closure1$_closure2$_closure4.doCall(main.nf.test) + at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke0(Native Method) + at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:77) + at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) + at java.base/java.lang.reflect.Method.invoke(Method.java:568) + at org.codehaus.groovy.reflection.CachedMethod.invoke(CachedMethod.java:107) + at groovy.lang.MetaMethod.doMethodInvoke(MetaMethod.java:323) + at org.codehaus.groovy.runtime.metaclass.ClosureMetaClass.invokeMethod(ClosureMetaClass.java:274) + at groovy.lang.MetaClassImpl.invokeMethod(MetaClassImpl.java:1030) + at groovy.lang.Closure.call(Closure.java:427) + at groovy.lang.Closure.call(Closure.java:406) + at com.askimed.nf.test.lang.TestCode.execute(TestCode.java:16) + at com.askimed.nf.test.lang.pipeline.PipelineTest.execute(PipelineTest.java:135) + at com.askimed.nf.test.core.TestExecutionEngine.execute(TestExecutionEngine.java:214) + at com.askimed.nf.test.commands.RunTestsCommand.execute(RunTestsCommand.java:184) + at com.askimed.nf.test.commands.AbstractCommand.call(AbstractCommand.java:43) + at com.askimed.nf.test.commands.AbstractCommand.call(AbstractCommand.java:18) + at picocli.CommandLine.executeUserObject(CommandLine.java:1953) + at picocli.CommandLine.access$1300(CommandLine.java:145) + at picocli.CommandLine$RunLast.executeUserObjectOfLastSubcommandWithSameParent(CommandLine.java:2352) + at picocli.CommandLine$RunLast.handle(CommandLine.java:2346) + at picocli.CommandLine$RunLast.handle(CommandLine.java:2311) + at picocli.CommandLine$AbstractParseResultHandler.execute(CommandLine.java:2179) + at picocli.CommandLine.execute(CommandLine.java:2078) + at com.askimed.nf.test.App.run(App.java:44) + at com.askimed.nf.test.App.main(App.java:51) +Aug-21 10:50:58.842 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Testsuite 'Test pipeline' finished. snapshot file: false, skipped tests: false, failed tests: true +Aug-21 10:50:58.842 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Executed 1 tests. 1 tests failed. Done! diff --git a/tests/main.nf.test b/tests/main.nf.test index 9014fad..ce70bbe 100644 --- a/tests/main.nf.test +++ b/tests/main.nf.test @@ -8,9 +8,11 @@ nextflow_pipeline { when { params { - input = 'assets/samplesheet_germline_hg38.csv' + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' sample = "HG002" - input = "assets/samplesheet_germline_hg38.csv" + input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_HG002_hg38.csv" outdir = "$outputDir" genome = 'GRCh38' analysis = 'germline' @@ -28,19 +30,8 @@ nextflow_pipeline { } then { - assert workflow.success - assertAll( - { assert new File("$outputDir/samplesheet/samplesheet.csv").readLines().size() == 15 }, - { assert new File("$outputDir/samplesheet/samplesheet.csv").readLines()*.split(',')[0].take(4) == ['"sample"', '"fastq_1"', '"fastq_2"', '"run_accession"'] }, - { assert new File("$outputDir/samplesheet/samplesheet.csv").readLines()*.split(',').collect { it[0] } == ['"sample"', '"DRX024467"', '"DRX026011"', '"ERX1234253"', '"SRX10940790"', '"SRX11047067"', '"SRX17709227"', '"SRX17709228"', '"SRX6725035"', '"SRX9315476"', '"SRX9504942"', '"SRX9504942"', '"SRX9504942"', '"SRX9504942"', '"SRX9626017"'] }, - { assert new File("$outputDir/samplesheet/samplesheet.csv").text.contains('Illumina HiSeq 2500') }, - { assert new File("$outputDir/fastq/DRX024467_DRR026872.fastq.gz").exists() }, - { assert new File("$outputDir/fastq/SRX10940790_SRR14593545_1.fastq.gz").exists() }, - { assert new File("$outputDir/pipeline_info/nf_core_fetchngs_software_mqc_versions.yml").exists() }, - { assert new File("$outputDir/samplesheet/id_mappings.csv").exists() }, - { assert new File("$outputDir/samplesheet/multiqc_config.yml").exists() }, - { assert new File("$outputDir/samplesheet/samplesheet.csv").exists() } + { assert workflow.success } ) } } diff --git a/tests/nextflow.config b/tests/nextflow.config deleted file mode 100644 index d75ad44..0000000 --- a/tests/nextflow.config +++ /dev/null @@ -1,27 +0,0 @@ -params { - // Base directory for nf-core/modules test data - modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/' - - // Base directory for nf-core/fetchngs test data - pipelines_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/pipelines/fetchngs/1.15.0/' -} - -// Impose sensible resource limits for testing -process { - withName: '.*' { - cpus = 2 - memory = 3.GB - time = 2.h - } -} - -// Impose same minimum Nextflow version as the pipeline for testing -manifest { - nextflowVersion = '!>=23.04.0' -} - -// Disable all Nextflow reporting options -timeline { enabled = false } -report { enabled = false } -trace { enabled = false } -dag { enabled = false } From 853c2470d566d04be9699220ddb156569a7c34ea Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 21 Aug 2024 11:39:39 +0000 Subject: [PATCH 173/292] remove .nf-test.log --- .gitignore | 3 ++- .nf-test.log | 54 ---------------------------------------------------- 2 files changed, 2 insertions(+), 55 deletions(-) delete mode 100644 .nf-test.log diff --git a/.gitignore b/.gitignore index 8d2b4f7..57b3201 100644 --- a/.gitignore +++ b/.gitignore @@ -11,5 +11,6 @@ test_sv*/ test/ null/* results*/* -.nf-test/* +.nf-test/ +.nf-test.log diff --git a/.nf-test.log b/.nf-test.log deleted file mode 100644 index e04c614..0000000 --- a/.nf-test.log +++ /dev/null @@ -1,54 +0,0 @@ -Aug-21 10:47:08.575 [main] INFO com.askimed.nf.test.App - nf-test 0.8.4 -Aug-21 10:47:08.597 [main] INFO com.askimed.nf.test.App - Arguments: [test, tests/main.nf.test, --profile, docker] -Aug-21 10:47:09.754 [main] INFO com.askimed.nf.test.App - Nextflow Version: 24.04.4 -Aug-21 10:47:09.758 [main] WARN com.askimed.nf.test.commands.RunTestsCommand - No nf-test config file found. -Aug-21 10:47:09.759 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Detected 1 test files. -Aug-21 10:47:10.462 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/workspace/variantbenchmarking/bin' to '/workspace/variantbenchmarking/.nf-test/tests/bf2b50ea975fb31273e738e1ac12ece5/meta/bin' -Aug-21 10:47:10.468 [main] WARN com.askimed.nf.test.config.FileStaging - File '/workspace/variantbenchmarking/lib' not found. Ignore it. -Aug-21 10:47:10.468 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/workspace/variantbenchmarking/assets' to '/workspace/variantbenchmarking/.nf-test/tests/bf2b50ea975fb31273e738e1ac12ece5/meta/assets' -Aug-21 10:47:10.482 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Started test plan -Aug-21 10:47:10.483 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Running testsuite 'Test pipeline' from file '/workspace/variantbenchmarking/tests/main.nf.test'. -Aug-21 10:47:10.483 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Run test 'bf2b50ea: Run with profile test'. type: com.askimed.nf.test.lang.pipeline.PipelineTest -Aug-21 10:50:58.838 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Test 'bf2b50ea: Run with profile test' finished. status: FAILED -org.codehaus.groovy.runtime.powerassert.PowerAssertionError: 1 of 1 assertions failed - at com.askimed.nf.test.lang.extensions.GlobalMethods.assertAll(GlobalMethods.java:48) - at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke0(Native Method) - at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:77) - at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) - at java.base/java.lang.reflect.Method.invoke(Method.java:568) - at org.codehaus.groovy.reflection.CachedMethod.invoke(CachedMethod.java:107) - at groovy.lang.MetaMethod.doMethodInvoke(MetaMethod.java:323) - at org.codehaus.groovy.runtime.callsite.StaticMetaMethodSite.invoke(StaticMetaMethodSite.java:44) - at org.codehaus.groovy.runtime.callsite.StaticMetaMethodSite.callStatic(StaticMetaMethodSite.java:100) - at org.codehaus.groovy.runtime.callsite.CallSiteArray.defaultCallStatic(CallSiteArray.java:55) - at org.codehaus.groovy.runtime.callsite.AbstractCallSite.callStatic(AbstractCallSite.java:217) - at org.codehaus.groovy.runtime.callsite.AbstractCallSite.callStatic(AbstractCallSite.java:231) - at main_nf$_run_closure1$_closure2$_closure4.doCall(main.nf.test:33) - at main_nf$_run_closure1$_closure2$_closure4.doCall(main.nf.test) - at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke0(Native Method) - at java.base/jdk.internal.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:77) - at java.base/jdk.internal.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43) - at java.base/java.lang.reflect.Method.invoke(Method.java:568) - at org.codehaus.groovy.reflection.CachedMethod.invoke(CachedMethod.java:107) - at groovy.lang.MetaMethod.doMethodInvoke(MetaMethod.java:323) - at org.codehaus.groovy.runtime.metaclass.ClosureMetaClass.invokeMethod(ClosureMetaClass.java:274) - at groovy.lang.MetaClassImpl.invokeMethod(MetaClassImpl.java:1030) - at groovy.lang.Closure.call(Closure.java:427) - at groovy.lang.Closure.call(Closure.java:406) - at com.askimed.nf.test.lang.TestCode.execute(TestCode.java:16) - at com.askimed.nf.test.lang.pipeline.PipelineTest.execute(PipelineTest.java:135) - at com.askimed.nf.test.core.TestExecutionEngine.execute(TestExecutionEngine.java:214) - at com.askimed.nf.test.commands.RunTestsCommand.execute(RunTestsCommand.java:184) - at com.askimed.nf.test.commands.AbstractCommand.call(AbstractCommand.java:43) - at com.askimed.nf.test.commands.AbstractCommand.call(AbstractCommand.java:18) - at picocli.CommandLine.executeUserObject(CommandLine.java:1953) - at picocli.CommandLine.access$1300(CommandLine.java:145) - at picocli.CommandLine$RunLast.executeUserObjectOfLastSubcommandWithSameParent(CommandLine.java:2352) - at picocli.CommandLine$RunLast.handle(CommandLine.java:2346) - at picocli.CommandLine$RunLast.handle(CommandLine.java:2311) - at picocli.CommandLine$AbstractParseResultHandler.execute(CommandLine.java:2179) - at picocli.CommandLine.execute(CommandLine.java:2078) - at com.askimed.nf.test.App.run(App.java:44) - at com.askimed.nf.test.App.main(App.java:51) -Aug-21 10:50:58.842 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Testsuite 'Test pipeline' finished. snapshot file: false, skipped tests: false, failed tests: true -Aug-21 10:50:58.842 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Executed 1 tests. 1 tests failed. Done! From 26573df99849dbbbc5d330104cbff121aba2ebd1 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 21 Aug 2024 11:42:30 +0000 Subject: [PATCH 174/292] fix email_template.html lint --- assets/email_template.html | 144 ++++++++++++++++++++++++++----------- 1 file changed, 102 insertions(+), 42 deletions(-) diff --git a/assets/email_template.html b/assets/email_template.html index afaac61..a8adb24 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,53 +1,113 @@ - - - - + + + + - - nf-core/variantbenchmarking Pipeline Report - - -

    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    - - <% out << summary.collect{ k,v -> "" }.join("\n") %> - -
    $k
    $v
    +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> " + + + + + " }.join("\n") %> + +
    + $k + + 
    $v
    +
    -

    nf-core/variantbenchmarking

    -

    https://github.com/nf-core/variantbenchmarking

    - - - - +

    nf-core/variantbenchmarking

    +

    https://github.com/nf-core/variantbenchmarking

    + + From e70ea8ba29dcf6325daa6922897846f9108a4e28 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 21 Aug 2024 11:54:13 +0000 Subject: [PATCH 175/292] second attempt to fix email_template.html --- assets/email_template.html | 144 +++++++++++-------------------------- 1 file changed, 42 insertions(+), 102 deletions(-) diff --git a/assets/email_template.html b/assets/email_template.html index a8adb24..de8972a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -1,113 +1,53 @@ - - - - + + + + - - nf-core/variantbenchmarking Pipeline Report - - -
    - + + nf-core/variantbenchmarking Pipeline Report + + +
    -

    nf-core/variantbenchmarking ${version}

    -

    Run Name: $runName

    + - <% if (!success){ out << """ -
    -

    nf-core/variantbenchmarking execution completed unsuccessfully!

    +

    nf-core/variantbenchmarking ${version}

    +

    Run Name: $runName

    + +<% if (!success){ + out << """ +
    +

    nf-core/variantbenchmarking execution completed unsuccessfully!

    The exit status of the task that caused the workflow execution to fail was: $exitStatus.

    The full error message was:

    - 
    ${errorReport}
    -
    - """ } else { out << """ -
    + 
    ${errorReport}
    +
    + """ +} else { + out << """ +
    nf-core/variantbenchmarking execution completed successfully! -
    - """ } %> +
    + """ +} +%> -

    The workflow was completed at $dateComplete (duration: $duration)

    -

    The command used to launch the workflow was as follows:

    - 
    -$commandLine
    +

    The workflow was completed at $dateComplete (duration: $duration)

    +

    The command used to launch the workflow was as follows:

    +
    $commandLine
    -

    Pipeline Configuration:

    - - - <% out << summary.collect{ k,v -> " - - - - - " }.join("\n") %> - -
    - $k - - 
    $v
    -
    +

    Pipeline Configuration:

    + + + <% out << summary.collect{ k,v -> "" }.join("\n") %> + +
    $k
    $v
    -

    nf-core/variantbenchmarking

    -

    https://github.com/nf-core/variantbenchmarking

    -
    - +

    nf-core/variantbenchmarking

    +

    https://github.com/nf-core/variantbenchmarking

    + +
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variants onlyAll variantsLEGENDOptional processesSmall variants onlySomatic variants onlyTruth preprocessingbcftools normprepysplitSNVsINDELsbcftools dedupfiltrationsvariant statistics--preprocessing--method--sv_standardizationstandardization--liftoversumary reportingTRUTH VCFs High Confidence Regionsv1.0.0chainindexgenomebedvcfvcffastachromosomprefixestxtchainrtg-tools sdfsdfreformatingrulesyamlstatsstatsrochtmlpngcsvvcf From 798627a8a873d305d0541cfab6ab1b88413c715b Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 8 Oct 2024 12:32:24 +0000 Subject: [PATCH 178/292] Template update for nf-core/tools version 3.0.0 --- .editorconfig | 4 + .github/CONTRIBUTING.md | 10 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 23 +- .github/workflows/ci.yml | 17 +- .github/workflows/download_pipeline.yml | 53 ++- .github/workflows/linting.yml | 23 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .../workflows/template_version_comment.yml | 43 ++ .gitpod.yml | 7 +- .nf-core.yml | 16 +- .pre-commit-config.yaml | 2 +- .prettierignore | 1 + CITATIONS.md | 4 +- README.md | 5 +- assets/email_template.html | 2 +- assets/schema_input.json | 2 +- conf/base.config | 34 +- conf/igenomes_ignored.config | 9 + conf/modules.config | 1 - conf/test.config | 13 +- docs/images/mqc_fastqc_adapter.png | Bin 23458 -> 0 bytes docs/images/mqc_fastqc_counts.png | Bin 33918 -> 0 bytes docs/images/mqc_fastqc_quality.png | Bin 55769 -> 0 bytes docs/output.md | 11 +- docs/usage.md | 12 +- main.nf | 10 +- modules.json | 12 +- modules/nf-core/fastqc/environment.yml | 2 - modules/nf-core/fastqc/main.nf | 5 +- modules/nf-core/fastqc/meta.yml | 57 +-- modules/nf-core/fastqc/tests/main.nf.test | 225 ++++++++--- .../nf-core/fastqc/tests/main.nf.test.snap | 370 ++++++++++++++++-- modules/nf-core/multiqc/environment.yml | 4 +- modules/nf-core/multiqc/main.nf | 14 +- modules/nf-core/multiqc/meta.yml | 78 ++-- modules/nf-core/multiqc/tests/main.nf.test | 8 + .../nf-core/multiqc/tests/main.nf.test.snap | 20 +- modules/nf-core/multiqc/tests/nextflow.config | 5 + nextflow.config | 148 ++++--- nextflow_schema.json | 87 +--- .../main.nf | 56 +-- .../nf-core/utils_nextflow_pipeline/main.nf | 24 +- .../tests/nextflow.config | 2 +- .../nf-core/utils_nfcore_pipeline/main.nf | 45 ++- .../nf-core/utils_nfschema_plugin/main.nf | 46 +++ .../nf-core/utils_nfschema_plugin/meta.yml | 35 ++ .../utils_nfschema_plugin/tests/main.nf.test | 117 ++++++ .../tests/nextflow.config | 8 + .../tests/nextflow_schema.json | 8 +- .../nf-core/utils_nfvalidation_plugin/main.nf | 62 --- .../utils_nfvalidation_plugin/meta.yml | 44 --- .../tests/main.nf.test | 200 ---------- .../utils_nfvalidation_plugin/tests/tags.yml | 2 - workflows/variantbenchmarking.nf | 23 +- 56 files changed, 1206 insertions(+), 809 deletions(-) create mode 100644 .github/workflows/template_version_comment.yml create mode 100644 conf/igenomes_ignored.config delete mode 100755 docs/images/mqc_fastqc_adapter.png delete mode 100755 docs/images/mqc_fastqc_counts.png delete mode 100755 docs/images/mqc_fastqc_quality.png create mode 100644 modules/nf-core/multiqc/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config rename subworkflows/nf-core/{utils_nfvalidation_plugin => utils_nfschema_plugin}/tests/nextflow_schema.json (95%) delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test delete mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 72dda28..e105881 100644 --- a/.editorconfig +++ b/.editorconfig @@ -11,6 +11,7 @@ indent_style = space [*.{md,yml,yaml,html,css,scss,js}] indent_size = 2 + # These files are edited and tested upstream in nf-core/modules [/modules/nf-core/**] charset = unset @@ -25,9 +26,12 @@ insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset + + [/assets/email*] indent_size = unset + # ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index e4be8b3..2f2ce78 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -19,7 +19,7 @@ If you'd like to write some code for nf-core/variantbenchmarking, the standard w 1. Check that there isn't already an issue about your idea in the [nf-core/variantbenchmarking issues](https://github.com/nf-core/variantbenchmarking/issues) to avoid duplicating work. If there isn't one already, please create one so that others know you're working on this 2. [Fork](https://help.github.com/en/github/getting-started-with-github/fork-a-repo) the [nf-core/variantbenchmarking repository](https://github.com/nf-core/variantbenchmarking) to your GitHub account 3. Make the necessary changes / additions within your forked repository following [Pipeline conventions](#pipeline-contribution-conventions) -4. Use `nf-core schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). +4. Use `nf-core pipelines schema build` and add any new parameters to the pipeline JSON schema (requires [nf-core tools](https://github.com/nf-core/tools) >= 1.10). 5. Submit a Pull Request against the `dev` branch and wait for the code to be reviewed and merged If you're not used to this workflow with git, you can start with some [docs from GitHub](https://help.github.com/en/github/collaborating-with-issues-and-pull-requests) or even their [excellent `git` resources](https://try.github.io/). @@ -40,7 +40,7 @@ There are typically two types of tests that run: ### Lint tests `nf-core` has a [set of guidelines](https://nf-co.re/developers/guidelines) which all pipelines must adhere to. -To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core lint ` command. +To enforce these and ensure that all pipelines stay in sync, we have developed a helper tool which runs checks on the pipeline code. This is in the [nf-core/tools repository](https://github.com/nf-core/tools) and once installed can be run locally with the `nf-core pipelines lint ` command. If any failures or warnings are encountered, please follow the listed URL for more documentation. @@ -75,7 +75,7 @@ If you wish to contribute a new step, please use the following coding standards: 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new parameters to `nextflow.config` with a default (see below). -5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core schema build` tool). +5. Add any new parameters to `nextflow_schema.json` with help text (via the `nf-core pipelines schema build` tool). 6. Add sanity checks and validation for all relevant parameters. 7. Perform local tests to validate that the new code works as expected. 8. If applicable, add a new test command in `.github/workflow/ci.yml`. @@ -86,7 +86,7 @@ If you wish to contribute a new step, please use the following coding standards: Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. -Once there, use `nf-core schema build` to add to `nextflow_schema.json`. +Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`. ### Default processes resource requirements @@ -103,7 +103,7 @@ Please use the following naming schemes, to make it easy to understand what is g ### Nextflow version bumping -If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core bump-version --nextflow . [min-nf-version]` +If you are using a new feature from core Nextflow, you may bump the minimum required version of nextflow in the pipeline with: `nf-core pipelines bump-version --nextflow . [min-nf-version]` ### Images and figures diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index b880d5e..e510d3e 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/vari - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/variantbenchmarking/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/variantbenchmarking _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. -- [ ] Make sure your code lints (`nf-core lint`). +- [ ] Make sure your code lints (`nf-core pipelines lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index fb2878b..9c77a26 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -1,18 +1,33 @@ name: nf-core AWS full size tests -# This workflow is triggered on published releases. +# This workflow is triggered on PRs opened against the master branch. # It can be additionally triggered manually with GitHub actions workflow dispatch button. # It runs the -profile 'test_full' on AWS batch on: - release: - types: [published] + pull_request: + branches: + - master workflow_dispatch: + pull_request_review: + types: [submitted] + jobs: run-platform: name: Run AWS full tests - if: github.repository == 'nf-core/variantbenchmarking' + if: github.repository == 'nf-core/variantbenchmarking' && github.event.review.state == 'approved' runs-on: ubuntu-latest steps: + - uses: octokit/request-action@v2.x + id: check_approvals + with: + route: GET /repos/${{ github.repository }}/pulls/${{ github.event.review.number }}/reviews + env: + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + - id: test_variables + run: | + JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}' + CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length') + test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 9d35013..7656200 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -7,6 +7,7 @@ on: pull_request: release: types: [published] + workflow_dispatch: env: NXF_ANSI_LOG: false @@ -24,7 +25,7 @@ jobs: strategy: matrix: NXF_VER: - - "23.04.0" + - "24.04.2" - "latest-everything" steps: - name: Check out pipeline code @@ -38,9 +39,21 @@ jobs: - name: Disk space cleanup uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - name: Run pipeline with test data + - name: Run pipeline with test data (docker) # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters # Remember that you can parallelise this by using strategy.matrix run: | nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + + - name: Run pipeline with test data (singularity) + # TODO nf-core: You can customise CI pipeline run tests as required + run: | + nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results + if: "${{ github.base_ref == 'master' }}" + + - name: Run pipeline with test data (conda) + # TODO nf-core: You can customise CI pipeline run tests as required + run: | + nextflow run ${GITHUB_WORKSPACE} -profile test,conda --outdir ./results + if: "${{ github.base_ref == 'master' }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 2d20d64..713dc3e 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -1,4 +1,4 @@ -name: Test successful pipeline download with 'nf-core download' +name: Test successful pipeline download with 'nf-core pipelines download' # Run the workflow when: # - dispatched manually @@ -8,7 +8,7 @@ on: workflow_dispatch: inputs: testbranch: - description: "The specific branch you wish to utilize for the test execution of nf-core download." + description: "The specific branch you wish to utilize for the test execution of nf-core pipelines download." required: true default: "dev" pull_request: @@ -39,9 +39,11 @@ jobs: with: python-version: "3.12" architecture: "x64" - - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 + + - name: Setup Apptainer + uses: eWaterCycle/setup-apptainer@4bb22c52d4f63406c49e94c804632975787312b3 # v2.0.0 with: - singularity-version: 3.8.3 + apptainer-version: 1.3.4 - name: Install dependencies run: | @@ -54,33 +56,64 @@ jobs: echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} + - name: Make a cache directory for the container images + run: | + mkdir -p ./singularity_container_images + - name: Download the pipeline env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core pipelines download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ --container-system 'singularity' \ - --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io" \ --container-cache-utilisation 'amend' \ - --download-configuration + --download-configuration 'yes' - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} + - name: Count the downloaded number of container images + id: count_initial + run: | + image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) + echo "Initial container image count: $image_count" + echo "IMAGE_COUNT_INITIAL=$image_count" >> ${GITHUB_ENV} + - name: Run the downloaded pipeline (stub) id: stub_run_pipeline continue-on-error: true env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results - name: Run the downloaded pipeline (stub run not supported) id: run_pipeline if: ${{ job.steps.stub_run_pipeline.status == failure() }} env: - NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results + + - name: Count the downloaded number of container images + id: count_afterwards + run: | + image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) + echo "Post-pipeline run container image count: $image_count" + echo "IMAGE_COUNT_AFTER=$image_count" >> ${GITHUB_ENV} + + - name: Compare container image counts + run: | + if [ "${{ env.IMAGE_COUNT_INITIAL }}" -ne "${{ env.IMAGE_COUNT_AFTER }}" ]; then + initial_count=${{ env.IMAGE_COUNT_INITIAL }} + final_count=${{ env.IMAGE_COUNT_AFTER }} + difference=$((final_count - initial_count)) + echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!" + tree ./singularity_container_images + exit 1 + else + echo "The pipeline can be downloaded successfully!" + fi diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 1fcafe8..b882838 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -1,6 +1,6 @@ name: nf-core linting # This workflow is triggered on pushes and PRs to the repository. -# It runs the `nf-core lint` and markdown lint tests to ensure +# It runs the `nf-core pipelines lint` and markdown lint tests to ensure # that the code meets the nf-core guidelines. on: push: @@ -41,17 +41,32 @@ jobs: python-version: "3.12" architecture: "x64" + - name: read .nf-core.yml + uses: pietrobolcato/action-read-yaml@1.0.0 + id: read_yml + with: + config: ${{ github.workspace }}/.nf-core.yaml + - name: Install dependencies run: | python -m pip install --upgrade pip - pip install nf-core + pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }} + + - name: Run nf-core pipelines lint + if: ${{ github.base_ref != 'master' }} + env: + GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} + GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} + GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} + run: nf-core -l lint_log.txt pipelines lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - - name: Run nf-core lint + - name: Run nf-core pipelines lint --release + if: ${{ github.base_ref == 'master' }} env: GITHUB_COMMENTS_URL: ${{ github.event.pull_request.comments_url }} GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} GITHUB_PR_COMMIT: ${{ github.event.pull_request.head.sha }} - run: nf-core -l lint_log.txt lint --dir ${GITHUB_WORKSPACE} --markdown lint_results.md + run: nf-core -l lint_log.txt pipelines lint --release --dir ${GITHUB_WORKSPACE} --markdown lint_results.md - name: Save PR number if: ${{ always() }} diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 40acc23..42e519b 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 + uses: dawidd6/action-download-artifact@bf251b5aa9c2f7eeb574a96ee720e24f801b7c11 # v6 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 03ecfcf..c6ba35d 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT + echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" | sed 's/-//g' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml new file mode 100644 index 0000000..9dea41f --- /dev/null +++ b/.github/workflows/template_version_comment.yml @@ -0,0 +1,43 @@ +name: nf-core template version comment +# This workflow is triggered on PRs to check if the pipeline template version matches the latest nf-core version. +# It posts a comment to the PR, even if it comes from a fork. + +on: pull_request_target + +jobs: + template_version: + runs-on: ubuntu-latest + steps: + - name: Check out pipeline code + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + + - name: Read template version from .nf-core.yml + uses: pietrobolcato/action-read-yaml@1.0.0 + id: read_yml + with: + config: ${{ github.workspace }}/.nf-core.yml + + - name: Install nf-core + run: | + python -m pip install --upgrade pip + pip install nf-core==${{ steps.read_yml.outputs['nf_core_version'] }} + + - name: Check nf-core outdated + id: nf_core_outdated + run: pip list --outdated | grep nf-core + + - name: Post nf-core template version comment + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 + if: | + ${{ steps.nf_core_outdated.outputs.stdout }} =~ 'nf-core' + with: + repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }} + allow-repeats: false + message: | + ## :warning: Newer version of the nf-core template is available. + + Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}. + Please update your pipeline to the latest version. + + For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync). + # diff --git a/.gitpod.yml b/.gitpod.yml index 105a182..4611863 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -4,17 +4,14 @@ tasks: command: | pre-commit install --install-hooks nextflow self-update - - name: unset JAVA_TOOL_OPTIONS - command: | - unset JAVA_TOOL_OPTIONS vscode: extensions: # based on nf-core.nf-core-extensionpack - - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code + #- esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code - charliermarsh.ruff # Code linter Ruff diff --git a/.nf-core.yml b/.nf-core.yml index e0b85a7..5124a75 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,2 +1,16 @@ +bump_version: null +lint: null +nf_core_version: 3.0.0 +org_path: null repository_type: pipeline -nf_core_version: "2.14.1" +template: + author: kuebra.narci@dkfz.de + description: "Benchmarking pipeline for germline and somatic variant callers " + force: false + is_nfcore: true + name: variantbenchmarking + org: nf-core + outdir: . + skip_features: null + version: 1.0dev +update: null diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 4dc0f1d..9e9f0e1 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -7,7 +7,7 @@ repos: - prettier@3.2.5 - repo: https://github.com/editorconfig-checker/editorconfig-checker.python - rev: "2.7.3" + rev: "3.0.3" hooks: - id: editorconfig-checker alias: ec diff --git a/.prettierignore b/.prettierignore index 437d763..610e506 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,3 +1,4 @@ + email_template.html adaptivecard.json slackreport.json diff --git a/CITATIONS.md b/CITATIONS.md index 28d5f49..44ca0a0 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,11 +12,11 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. +> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) - > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. +> Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. ## Software packaging/containerisation tools diff --git a/README.md b/README.md index 72791aa..13a69bf 100644 --- a/README.md +++ b/README.md @@ -9,7 +9,7 @@ [![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -67,8 +67,7 @@ nextflow run nf-core/variantbenchmarking \ ``` > [!WARNING] -> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; -> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files). For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). diff --git a/assets/email_template.html b/assets/email_template.html index afaac61..de8972a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + nf-core/variantbenchmarking Pipeline Report diff --git a/assets/schema_input.json b/assets/schema_input.json index 19da518..51611c0 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -1,5 +1,5 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/assets/schema_input.json", "title": "nf-core/variantbenchmarking pipeline - params.input schema", "description": "Schema for the file provided with params.input", diff --git a/conf/base.config b/conf/base.config index 42d7cd3..135e755 100644 --- a/conf/base.config +++ b/conf/base.config @@ -11,9 +11,9 @@ process { // TODO nf-core: Check the defaults for all processes - cpus = { check_max( 1 * task.attempt, 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 1 * task.attempt } + memory = { 6.GB * task.attempt } + time = { 4.h * task.attempt } errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' } maxRetries = 1 @@ -27,30 +27,30 @@ process { // TODO nf-core: Customise requirements for specific processes. // See https://www.nextflow.io/docs/latest/config.html#config-process-selectors withLabel:process_single { - cpus = { check_max( 1 , 'cpus' ) } - memory = { check_max( 6.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 1 } + memory = { 6.GB * task.attempt } + time = { 4.h * task.attempt } } withLabel:process_low { - cpus = { check_max( 2 * task.attempt, 'cpus' ) } - memory = { check_max( 12.GB * task.attempt, 'memory' ) } - time = { check_max( 4.h * task.attempt, 'time' ) } + cpus = { 2 * task.attempt } + memory = { 12.GB * task.attempt } + time = { 4.h * task.attempt } } withLabel:process_medium { - cpus = { check_max( 6 * task.attempt, 'cpus' ) } - memory = { check_max( 36.GB * task.attempt, 'memory' ) } - time = { check_max( 8.h * task.attempt, 'time' ) } + cpus = { 6 * task.attempt } + memory = { 36.GB * task.attempt } + time = { 8.h * task.attempt } } withLabel:process_high { - cpus = { check_max( 12 * task.attempt, 'cpus' ) } - memory = { check_max( 72.GB * task.attempt, 'memory' ) } - time = { check_max( 16.h * task.attempt, 'time' ) } + cpus = { 12 * task.attempt } + memory = { 72.GB * task.attempt } + time = { 16.h * task.attempt } } withLabel:process_long { - time = { check_max( 20.h * task.attempt, 'time' ) } + time = { 20.h * task.attempt } } withLabel:process_high_memory { - memory = { check_max( 200.GB * task.attempt, 'memory' ) } + memory = { 200.GB * task.attempt } } withLabel:error_ignore { errorStrategy = 'ignore' diff --git a/conf/igenomes_ignored.config b/conf/igenomes_ignored.config new file mode 100644 index 0000000..b4034d8 --- /dev/null +++ b/conf/igenomes_ignored.config @@ -0,0 +1,9 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for iGenomes paths +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Empty genomes dictionary to use when igenomes is ignored. +---------------------------------------------------------------------------------------- +*/ + +params.genomes = [:] diff --git a/conf/modules.config b/conf/modules.config index d203d2b..d266a38 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -21,7 +21,6 @@ process { withName: FASTQC { ext.args = '--quiet' } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/conf/test.config b/conf/test.config index 824b816..1adb3c8 100644 --- a/conf/test.config +++ b/conf/test.config @@ -10,15 +10,18 @@ ---------------------------------------------------------------------------------------- */ +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} + params { config_profile_name = 'Test profile' config_profile_description = 'Minimal test dataset to check pipeline function' - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.GB' - max_time = '6.h' - // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed diff --git a/docs/images/mqc_fastqc_adapter.png b/docs/images/mqc_fastqc_adapter.png deleted file mode 100755 index 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zXwhtT%ei{n#FzEH|C;yZ>+$!u_x#*+`=L8{b9SH^9&27u3G_Gxqxe`L2UJtdxghk z&-wzDFvLvW{chK5u3{n6GSKKy!P&C6w^IFpbD0bcp^A{{2lcLh_DXj@ybtYvc^;(2 M)78&qol`;+0Fu7JivR!s diff --git a/docs/output.md b/docs/output.md index c671e3f..9ee97dd 100644 --- a/docs/output.md +++ b/docs/output.md @@ -14,6 +14,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [FastQC](#fastqc) - Raw read QC - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline + - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ### FastQC @@ -29,16 +30,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). -![MultiQC - FastQC sequence counts plot](images/mqc_fastqc_counts.png) - -![MultiQC - FastQC mean quality scores plot](images/mqc_fastqc_quality.png) - -![MultiQC - FastQC adapter content plot](images/mqc_fastqc_adapter.png) - -:::note -The FastQC plots displayed in the MultiQC report shows _untrimmed_ reads. They may contain adapter sequence and potentially regions with low quality. -::: - ### MultiQC

    diff --git a/docs/usage.md b/docs/usage.md index 63d5e6b..c7a679e 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -85,9 +85,9 @@ The above pipeline run specified with a params file in yaml format: nextflow run nf-core/variantbenchmarking -profile docker -params-file params.yaml ``` -with `params.yaml` containing: +with: -```yaml +```yaml title="params.yaml" input: './samplesheet.csv' outdir: './results/' genome: 'GRCh37' @@ -199,14 +199,6 @@ See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). -## Azure Resource Requests - -To be used with the `azurebatch` profile by specifying the `-profile azurebatch`. -We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required. - -Note that the choice of VM size depends on your quota and the overall workload during the analysis. -For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes). - ## Running in the background Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. diff --git a/main.nf b/main.nf index 3633aec..59f6c99 100644 --- a/main.nf +++ b/main.nf @@ -9,8 +9,6 @@ ---------------------------------------------------------------------------------------- */ -nextflow.enable.dsl = 2 - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS @@ -20,7 +18,6 @@ nextflow.enable.dsl = 2 include { VARIANTBENCHMARKING } from './workflows/variantbenchmarking' include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' - include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' /* @@ -56,10 +53,8 @@ workflow NFCORE_VARIANTBENCHMARKING { VARIANTBENCHMARKING ( samplesheet ) - emit: multiqc_report = VARIANTBENCHMARKING.out.multiqc_report // channel: /path/to/multiqc_report.html - } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -70,27 +65,24 @@ workflow NFCORE_VARIANTBENCHMARKING { workflow { main: - // // SUBWORKFLOW: Run initialisation tasks // PIPELINE_INITIALISATION ( params.version, - params.help, params.validate_params, params.monochrome_logs, args, params.outdir, params.input ) - + // // WORKFLOW: Run main workflow // NFCORE_VARIANTBENCHMARKING ( PIPELINE_INITIALISATION.out.samplesheet ) - // // SUBWORKFLOW: Run completion tasks // diff --git a/modules.json b/modules.json index 6140044..550f632 100644 --- a/modules.json +++ b/modules.json @@ -7,12 +7,12 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "d20fb2a9cc3e2835e9d067d1046a63252eb17352", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "git_sha": "2fdce49d30c0254f76bc0f13c55c17455c1251ab", "installed_by": ["subworkflows"] }, - "utils_nfvalidation_plugin": { + "utils_nfschema_plugin": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml index 1787b38..691d4c7 100644 --- a/modules/nf-core/fastqc/environment.yml +++ b/modules/nf-core/fastqc/environment.yml @@ -1,7 +1,5 @@ -name: fastqc channels: - conda-forge - bioconda - - defaults dependencies: - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index d79f1c8..d8989f4 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -26,7 +26,10 @@ process FASTQC { def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') - def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory) + // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222 + // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus // FastQC memory value allowed range (100 - 10000) def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index ee5507e..4827da7 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -16,35 +16,44 @@ tools: homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ documentation: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/ licence: ["GPL-2.0-only"] + identifier: biotools:fastqc input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - reads: - type: file - description: | - List of input FastQ files of size 1 and 2 for single-end and paired-end data, - respectively. + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - html: - type: file - description: FastQC report - pattern: "*_{fastqc.html}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.html": + type: file + description: FastQC report + pattern: "*_{fastqc.html}" - zip: - type: file - description: FastQC report archive - pattern: "*_{fastqc.zip}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.zip": + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@drpatelh" - "@grst" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 70edae4..e9d79a0 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -23,17 +23,14 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. - // looks like this:
    Mon 2 Oct 2023
    test.gz
    - // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_single") } + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
    Mon 2 Oct 2023
    test.gz
    + // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -54,16 +51,14 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_paired") } + { assert process.success }, + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -83,13 +78,11 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -109,13 +102,11 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_bam") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -138,22 +129,20 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, - { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, - { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, - { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, - { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, - { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, - { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, - { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, - { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, - { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } + { assert process.success }, + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, + { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } @@ -173,21 +162,18 @@ nextflow_process { then { assertAll ( - { assert process.success }, - - { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, - { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, - { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - - { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } + { assert process.success }, + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match() } ) } } test("sarscov2 single-end [fastq] - stub") { - options "-stub" - + options "-stub" when { process { """ @@ -201,12 +187,123 @@ nextflow_process { then { assertAll ( - { assert process.success }, - { assert snapshot(process.out.html.collect { file(it[1]).getName() } + - process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match("fastqc_stub") } + { assert process.success }, + { assert snapshot(process.out).match() } ) } } + test("sarscov2 paired-end [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 interleaved [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 paired-end [bam] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 multiple [fastq] - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("sarscov2 custom_prefix - stub") { + + options "-stub" + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 86f7c31..d5db309 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,88 +1,392 @@ { - "fastqc_versions_interleaved": { + "sarscov2 custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:07.293713" + "timestamp": "2024-07-22T11:02:16.374038" }, - "fastqc_stub": { + "sarscov2 single-end [fastq] - stub": { "content": [ - [ - "test.html", - "test.zip", - "versions.yml:md5,e1cc25ca8af856014824abd842e93978" - ] + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": true + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": true + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:24.993809" + }, + "sarscov2 custom_prefix - stub": { + "content": [ + { + "0": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "mysample", + "single_end": true + }, + "mysample.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:31:01.425198" + "timestamp": "2024-07-22T11:03:10.93942" }, - "fastqc_versions_multiple": { + "sarscov2 interleaved [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:55.797907" + "timestamp": "2024-07-22T11:01:42.355718" }, - "fastqc_versions_bam": { + "sarscov2 paired-end [bam]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:40:26.795862" + "timestamp": "2024-07-22T11:01:53.276274" }, - "fastqc_versions_single": { + "sarscov2 multiple [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:39:27.043675" + "timestamp": "2024-07-22T11:02:05.527626" }, - "fastqc_versions_paired": { + "sarscov2 paired-end [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:01:31.188871" + }, + "sarscov2 paired-end [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:34.273566" + }, + "sarscov2 multiple [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:39:47.584191" + "timestamp": "2024-07-22T11:03:02.304411" }, - "fastqc_versions_custom_prefix": { + "sarscov2 single-end [fastq]": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:01:19.095607" + }, + "sarscov2 interleaved [fastq] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" + }, + "timestamp": "2024-07-22T11:02:44.640184" + }, + "sarscov2 paired-end [bam] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "html": [ + [ + { + "id": "test", + "single_end": false + }, + "test.html:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ], + "zip": [ + [ + { + "id": "test", + "single_end": false + }, + "test.zip:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.3" }, - "timestamp": "2024-01-31T17:41:14.576531" + "timestamp": "2024-07-22T11:02:53.550742" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index ca39fb6..f1cd99b 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -1,7 +1,5 @@ -name: multiqc channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::multiqc=1.21 + - bioconda::multiqc=1.24.1 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 47ac352..b9ccebd 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,14 +3,16 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : - 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.25--pyhdfd78af_0' : + 'biocontainers/multiqc:1.25--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" path(multiqc_config) path(extra_multiqc_config) path(multiqc_logo) + path(replace_names) + path(sample_names) output: path "*multiqc_report.html", emit: report @@ -23,16 +25,22 @@ process MULTIQC { script: def args = task.ext.args ?: '' + def prefix = task.ext.prefix ? "--filename ${task.ext.prefix}.html" : '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' - def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' + def logo = multiqc_logo ? "--cl-config 'custom_logo: \"${multiqc_logo}\"'" : '' + def replace = replace_names ? "--replace-names ${replace_names}" : '' + def samples = sample_names ? "--sample-names ${sample_names}" : '' """ multiqc \\ --force \\ $args \\ $config \\ + $prefix \\ $extra_config \\ $logo \\ + $replace \\ + $samples \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index 45a9bc3..b16c187 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,6 @@ name: multiqc -description: Aggregate results from bioinformatics analyses across many samples into a single report +description: Aggregate results from bioinformatics analyses across many samples into + a single report keywords: - QC - bioinformatics tools @@ -12,40 +13,59 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] + identifier: biotools:multiqc input: - - multiqc_files: - type: file - description: | - List of reports / files recognised by MultiQC, for example the html and zip output of FastQC - - multiqc_config: - type: file - description: Optional config yml for MultiQC - pattern: "*.{yml,yaml}" - - extra_multiqc_config: - type: file - description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. - pattern: "*.{yml,yaml}" - - multiqc_logo: - type: file - description: Optional logo file for MultiQC - pattern: "*.{png}" + - - multiqc_files: + type: file + description: | + List of reports / files recognised by MultiQC, for example the html and zip output of FastQC + - - multiqc_config: + type: file + description: Optional config yml for MultiQC + pattern: "*.{yml,yaml}" + - - extra_multiqc_config: + type: file + description: Second optional config yml for MultiQC. Will override common sections + in multiqc_config. + pattern: "*.{yml,yaml}" + - - multiqc_logo: + type: file + description: Optional logo file for MultiQC + pattern: "*.{png}" + - - replace_names: + type: file + description: | + Optional two-column sample renaming file. First column a set of + patterns, second column a set of corresponding replacements. Passed via + MultiQC's `--replace-names` option. + pattern: "*.{tsv}" + - - sample_names: + type: file + description: | + Optional TSV file with headers, passed to the MultiQC --sample_names + argument. + pattern: "*.{tsv}" output: - report: - type: file - description: MultiQC report file - pattern: "multiqc_report.html" + - "*multiqc_report.html": + type: file + description: MultiQC report file + pattern: "multiqc_report.html" - data: - type: directory - description: MultiQC data dir - pattern: "multiqc_data" + - "*_data": + type: directory + description: MultiQC data dir + pattern: "multiqc_data" - plots: - type: file - description: Plots created by MultiQC - pattern: "*_data" + - "*_plots": + type: file + description: Plots created by MultiQC + pattern: "*_data" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@abhi18av" - "@bunop" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index f1c4242..33316a7 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -8,6 +8,8 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" + config "./nextflow.config" + test("sarscov2 single-end [fastqc]") { when { @@ -17,6 +19,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -41,6 +45,8 @@ nextflow_process { input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } @@ -66,6 +72,8 @@ nextflow_process { input[1] = [] input[2] = [] input[3] = [] + input[4] = [] + input[5] = [] """ } } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index bfebd80..b779e46 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:48:55.657331" + "timestamp": "2024-07-10T12:41:34.562023" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:49:49.071937" + "timestamp": "2024-07-10T11:27:11.933869532" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" + "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd" ] ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.10.1" + "nextflow": "24.04.2" }, - "timestamp": "2024-02-29T08:49:25.457567" + "timestamp": "2024-07-10T11:26:56.709849369" } -} \ No newline at end of file +} diff --git a/modules/nf-core/multiqc/tests/nextflow.config b/modules/nf-core/multiqc/tests/nextflow.config new file mode 100644 index 0000000..c537a6a --- /dev/null +++ b/modules/nf-core/multiqc/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: 'MULTIQC' { + ext.prefix = null + } +} diff --git a/nextflow.config b/nextflow.config index 5806f13..09ac17e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,7 +16,6 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - // MultiQC options multiqc_config = null multiqc_title = null @@ -33,48 +32,26 @@ params { monochrome_logs = false hook_url = null help = false + help_full = false + show_hidden = false version = false pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' - // Config options config_profile_name = null config_profile_description = null + custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - - // Max resource options - // Defaults only, expecting to be overwritten - max_memory = '128.GB' - max_cpus = 16 - max_time = '240.h' - // Schema validation default options - validationFailUnrecognisedParams = false - validationLenientMode = false - validationSchemaIgnoreParams = 'genomes,igenomes_base' - validationShowHiddenParams = false - validate_params = true - + validate_params = true + } // Load base.config by default for all pipelines includeConfig 'conf/base.config' -// Load nf-core custom profiles from different Institutions -try { - includeConfig "${params.custom_config_base}/nfcore_custom.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config profiles: ${params.custom_config_base}/nfcore_custom.config") -} - -// Load nf-core/variantbenchmarking custom profiles from different institutions. -try { - includeConfig "${params.custom_config_base}/pipeline/variantbenchmarking.config" -} catch (Exception e) { - System.err.println("WARNING: Could not load nf-core/config/variantbenchmarking profiles: ${params.custom_config_base}/pipeline/variantbenchmarking.config") -} profiles { debug { dumpHashes = true @@ -89,7 +66,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false - conda.channels = ['conda-forge', 'bioconda', 'defaults'] + conda.channels = ['conda-forge', 'bioconda'] apptainer.enabled = false } mamba { @@ -178,25 +155,23 @@ profiles { test_full { includeConfig 'conf/test_full.config' } } -// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile -// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled -// Set to your registry if you have a mirror of containers -apptainer.registry = 'quay.io' -docker.registry = 'quay.io' -podman.registry = 'quay.io' -singularity.registry = 'quay.io' +// Load nf-core custom profiles from different Institutions +includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null" -// Nextflow plugins -plugins { - id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet -} +// Load nf-core/variantbenchmarking custom profiles from different institutions. +// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs +// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/variantbenchmarking.config" : "/dev/null" +// Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile +// Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled +// Set to your registry if you have a mirror of containers +apptainer.registry = 'quay.io' +docker.registry = 'quay.io' +podman.registry = 'quay.io' +singularity.registry = 'quay.io' +charliecloud.registry = 'quay.io' // Load igenomes.config if required -if (!params.igenomes_ignore) { - includeConfig 'conf/igenomes.config' -} else { - params.genomes = [:] -} +includeConfig !params.igenomes_ignore ? 'conf/igenomes.config' : 'conf/igenomes_ignored.config' // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. @@ -208,8 +183,15 @@ env { JULIA_DEPOT_PATH = "/usr/local/share/julia" } -// Capture exit codes from upstream processes when piping -process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Set bash options +process.shell = """\ +bash + +set -e # Exit if a tool returns a non-zero status/exit code +set -u # Treat unset variables and parameters as an error +set -o pipefail # Returns the status of the last command to exit with a non-zero status or zero if all successfully execute +set -C # No clobber - prevent output redirection from overwriting files. +""" // Disable process selector warnings by default. Use debug profile to enable warnings. nextflow.enable.configProcessNamesValidation = false @@ -236,45 +218,49 @@ manifest { name = 'nf-core/variantbenchmarking' author = """kuebra.narci@dkfz.de""" homePage = 'https://github.com/nf-core/variantbenchmarking' - description = """Master benchmarking pipeline for Structural Variant callers """ + description = """Benchmarking pipeline for germline and somatic variant callers """ mainScript = 'main.nf' - nextflowVersion = '!>=23.04.0' + nextflowVersion = '!>=24.04.2' version = '1.0dev' doi = '' } -// Load modules.config for DSL2 module specific options -includeConfig 'conf/modules.config' +// Nextflow plugins +plugins { + id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet +} + +validation { + defaultIgnoreParams = ["genomes"] + help { + enabled = true + command = "nextflow run $manifest.name -profile --input samplesheet.csv --outdir " + fullParameter = "help_full" + showHiddenParameter = "show_hidden" + beforeText = """ +-\033[2m----------------------------------------------------\033[0m- + \033[0;32m,--.\033[0;30m/\033[0;32m,-.\033[0m +\033[0;34m ___ __ __ __ ___ \033[0;32m/,-._.--~\'\033[0m +\033[0;34m |\\ | |__ __ / ` / \\ |__) |__ \033[0;33m} {\033[0m +\033[0;34m | \\| | \\__, \\__/ | \\ |___ \033[0;32m\\`-._,-`-,\033[0m + \033[0;32m`._,._,\'\033[0m +\033[0;35m ${manifest.name} ${manifest.version}\033[0m +-\033[2m----------------------------------------------------\033[0m- +""" + afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { " https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""} +* The nf-core framework + https://doi.org/10.1038/s41587-020-0439-x -// Function to ensure that resource requirements don't go beyond -// a maximum limit -def check_max(obj, type) { - if (type == 'memory') { - try { - if (obj.compareTo(params.max_memory as nextflow.util.MemoryUnit) == 1) - return params.max_memory as nextflow.util.MemoryUnit - else - return obj - } catch (all) { - println " ### ERROR ### Max memory '${params.max_memory}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'time') { - try { - if (obj.compareTo(params.max_time as nextflow.util.Duration) == 1) - return params.max_time as nextflow.util.Duration - else - return obj - } catch (all) { - println " ### ERROR ### Max time '${params.max_time}' is not valid! Using default value: $obj" - return obj - } - } else if (type == 'cpus') { - try { - return Math.min( obj, params.max_cpus as int ) - } catch (all) { - println " ### ERROR ### Max cpus '${params.max_cpus}' is not valid! Using default value: $obj" - return obj - } +* Software dependencies + https://github.com/${manifest.name}/blob/master/CITATIONS.md +""" + } + summary { + beforeText = validation.help.beforeText + afterText = validation.help.afterText } } + +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' + diff --git a/nextflow_schema.json b/nextflow_schema.json index 60ced44..e98f7f5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -1,10 +1,10 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", "title": "nf-core/variantbenchmarking pipeline parameters", - "description": "Master benchmarking pipeline for Structural Variant callers ", + "description": "Benchmarking pipeline for germline and somatic variant callers ", "type": "object", - "definitions": { + "$defs": { "input_output_options": { "title": "Input/output options", "type": "object", @@ -71,6 +71,14 @@ "fa_icon": "fas fa-ban", "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." + }, + "igenomes_base": { + "type": "string", + "format": "directory-path", + "description": "The base path to the igenomes reference files", + "fa_icon": "fas fa-ban", + "hidden": true, + "default": "s3://ngi-igenomes/igenomes/" } } }, @@ -122,41 +130,6 @@ } } }, - "max_job_request_options": { - "title": "Max job request options", - "type": "object", - "fa_icon": "fab fa-acquisitions-incorporated", - "description": "Set the top limit for requested resources for any single job.", - "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", - "properties": { - "max_cpus": { - "type": "integer", - "description": "Maximum number of CPUs that can be requested for any single job.", - "default": 16, - "fa_icon": "fas fa-microchip", - "hidden": true, - "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" - }, - "max_memory": { - "type": "string", - "description": "Maximum amount of memory that can be requested for any single job.", - "default": "128.GB", - "fa_icon": "fas fa-memory", - "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", - "hidden": true, - "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" - }, - "max_time": { - "type": "string", - "description": "Maximum amount of time that can be requested for any single job.", - "default": "240.h", - "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", - "hidden": true, - "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" - } - } - }, "generic_options": { "title": "Generic options", "type": "object", @@ -164,12 +137,6 @@ "description": "Less common options for the pipeline, typically set in a config file.", "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", "properties": { - "help": { - "type": "boolean", - "description": "Display help text.", - "fa_icon": "fas fa-question-circle", - "hidden": true - }, "version": { "type": "boolean", "description": "Display version and exit.", @@ -245,27 +212,6 @@ "fa_icon": "fas fa-check-square", "hidden": true }, - "validationShowHiddenParams": { - "type": "boolean", - "fa_icon": "far fa-eye-slash", - "description": "Show all params when using `--help`", - "hidden": true, - "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "validationFailUnrecognisedParams": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters fails when an unrecognised parameter is found.", - "hidden": true, - "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." - }, - "validationLenientMode": { - "type": "boolean", - "fa_icon": "far fa-check-circle", - "description": "Validation of parameters in lenient more.", - "hidden": true, - "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." - }, "pipelines_testdata_base_path": { "type": "string", "fa_icon": "far fa-check-circle", @@ -278,19 +224,16 @@ }, "allOf": [ { - "$ref": "#/definitions/input_output_options" - }, - { - "$ref": "#/definitions/reference_genome_options" + "$ref": "#/$defs/input_output_options" }, { - "$ref": "#/definitions/institutional_config_options" + "$ref": "#/$defs/reference_genome_options" }, { - "$ref": "#/definitions/max_job_request_options" + "$ref": "#/$defs/institutional_config_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/$defs/generic_options" } ] } diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index fa210e5..08a15f4 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -8,17 +8,14 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' -include { paramsSummaryMap } from 'plugin/nf-validation' -include { fromSamplesheet } from 'plugin/nf-validation' -include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { UTILS_NFSCHEMA_PLUGIN } from '../../nf-core/utils_nfschema_plugin' +include { paramsSummaryMap } from 'plugin/nf-schema' +include { samplesheetToList } from 'plugin/nf-schema' include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' -include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' -include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' include { imNotification } from '../../nf-core/utils_nfcore_pipeline' include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' -include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' /* ======================================================================================== @@ -30,7 +27,6 @@ workflow PIPELINE_INITIALISATION { take: version // boolean: Display version and exit - help // boolean: Display help text validate_params // boolean: Boolean whether to validate parameters against the schema at runtime monochrome_logs // boolean: Do not use coloured log outputs nextflow_cli_args // array: List of positional nextflow CLI args @@ -51,20 +47,16 @@ workflow PIPELINE_INITIALISATION { workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 ) + // // Validate parameters and generate parameter summary to stdout // - pre_help_text = nfCoreLogo(monochrome_logs) - post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) - def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " - UTILS_NFVALIDATION_PLUGIN ( - help, - workflow_command, - pre_help_text, - post_help_text, + UTILS_NFSCHEMA_PLUGIN ( + workflow, validate_params, - "nextflow_schema.json" + null ) + // // Check config provided to the pipeline @@ -80,8 +72,9 @@ workflow PIPELINE_INITIALISATION { // // Create channel from input file provided through params.input // + Channel - .fromSamplesheet("input") + .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json")) .map { meta, fastq_1, fastq_2 -> if (!fastq_2) { @@ -91,8 +84,8 @@ workflow PIPELINE_INITIALISATION { } } .groupTuple() - .map { - validateInputSamplesheet(it) + .map { samplesheet -> + validateInputSamplesheet(samplesheet) } .map { meta, fastqs -> @@ -117,13 +110,13 @@ workflow PIPELINE_COMPLETION { email // string: email address email_on_fail // string: email address sent on pipeline failure plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published monochrome_logs // boolean: Disable ANSI colour codes in log output hook_url // string: hook URL for notifications multiqc_report // string: Path to MultiQC report main: - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") // @@ -131,11 +124,18 @@ workflow PIPELINE_COMPLETION { // workflow.onComplete { if (email || email_on_fail) { - completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + completionEmail( + summary_params, + email, + email_on_fail, + plaintext_email, + outdir, + monochrome_logs, + multiqc_report.toList() + ) } completionSummary(monochrome_logs) - if (hook_url) { imNotification(summary_params, hook_url) } @@ -165,7 +165,7 @@ def validateInputSamplesheet(input) { def (metas, fastqs) = input[1..2] // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end - def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + def endedness_ok = metas.collect{ meta -> meta.single_end }.unique().size == 1 if (!endedness_ok) { error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") } @@ -197,7 +197,6 @@ def genomeExistsError() { error(error_string) } } - // // Generate methods description for MultiQC // @@ -239,8 +238,10 @@ def methodsDescriptionText(mqc_methods_yaml) { // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list def temp_doi_ref = "" - String[] manifest_doi = meta.manifest_map.doi.tokenize(",") - for (String doi_ref: manifest_doi) temp_doi_ref += "(doi:     ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + def manifest_doi = meta.manifest_map.doi.tokenize(",") + manifest_doi.each { doi_ref -> + temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + } meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) } else meta["doi_text"] = "" meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " @@ -261,3 +262,4 @@ def methodsDescriptionText(mqc_methods_yaml) { return description_html.toString() } + diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index ac31f28..28e32b2 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -2,10 +2,6 @@ // Subworkflow with functionality that may be useful for any Nextflow pipeline // -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - /* ======================================================================================== SUBWORKFLOW DEFINITION @@ -58,7 +54,7 @@ workflow UTILS_NEXTFLOW_PIPELINE { // Generate version string // def getWorkflowVersion() { - String version_string = "" + def version_string = "" as String if (workflow.manifest.version) { def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' version_string += "${prefix_v}${workflow.manifest.version}" @@ -79,10 +75,10 @@ def dumpParametersToJSON(outdir) { def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') def filename = "params_${timestamp}.json" def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) + def jsonStr = groovy.json.JsonOutput.toJson(params) + temp_pf.text = groovy.json.JsonOutput.prettyPrint(jsonStr) - FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") temp_pf.delete() } @@ -90,7 +86,7 @@ def dumpParametersToJSON(outdir) { // When running with -profile conda, warn if channels have not been set-up appropriately // def checkCondaChannels() { - Yaml parser = new Yaml() + def parser = new org.yaml.snakeyaml.Yaml() def channels = [] try { def config = parser.load("conda config --show channels".execute().text) @@ -102,14 +98,16 @@ def checkCondaChannels() { // Check that all channels are present // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def required_channels_in_order = ['conda-forge', 'bioconda'] def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + + required_channels_in_order.eachWithIndex { channel, index -> + if (index < required_channels_in_order.size() - 1) { + channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index+1])) + } } if (channels_missing | channel_priority_violation) { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index d0a926b..a09572e 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -3,7 +3,7 @@ manifest { author = """nf-core""" homePage = 'https://127.0.0.1' description = """Dummy pipeline""" - nextflowVersion = '!>=23.04.0' + nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index 14558c3..cbd8495 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -2,9 +2,6 @@ // Subworkflow with utility functions specific to the nf-core pipeline template // -import org.yaml.snakeyaml.Yaml -import nextflow.extension.FilesEx - /* ======================================================================================== SUBWORKFLOW DEFINITION @@ -34,7 +31,7 @@ workflow UTILS_NFCORE_PIPELINE { // Warn if a -profile or Nextflow config has not been provided to run the pipeline // def checkConfigProvided() { - valid_config = true + def valid_config = true as Boolean if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + @@ -66,11 +63,13 @@ def checkProfileProvided(nextflow_cli_args) { // def workflowCitation() { def temp_doi_ref = "" - String[] manifest_doi = workflow.manifest.doi.tokenize(",") + def manifest_doi = workflow.manifest.doi.tokenize(",") // Using a loop to handle multiple DOIs // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list - for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + manifest_doi.each { doi_ref -> + temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + } return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + @@ -84,7 +83,7 @@ def workflowCitation() { // Generate workflow version string // def getWorkflowVersion() { - String version_string = "" + def version_string = "" as String if (workflow.manifest.version) { def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' version_string += "${prefix_v}${workflow.manifest.version}" @@ -102,8 +101,8 @@ def getWorkflowVersion() { // Get software versions for pipeline // def processVersionsFromYAML(yaml_file) { - Yaml yaml = new Yaml() - versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + def yaml = new org.yaml.snakeyaml.Yaml() + def versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } return yaml.dumpAsMap(versions).trim() } @@ -124,7 +123,7 @@ def workflowVersionToYAML() { def softwareVersionsToYAML(ch_versions) { return ch_versions .unique() - .map { processVersionsFromYAML(it) } + .map { version -> processVersionsFromYAML(version) } .unique() .mix(Channel.of(workflowVersionToYAML())) } @@ -134,19 +133,19 @@ def softwareVersionsToYAML(ch_versions) { // def paramsSummaryMultiqc(summary_params) { def summary_section = '' - for (group in summary_params.keySet()) { + summary_params.keySet().each { group -> def group_params = summary_params.get(group) // This gets the parameters of that particular group if (group_params) { summary_section += "

    $group

    \n" summary_section += "
    \n" - for (param in group_params.keySet()) { + group_params.keySet().sort().each { param -> summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" } summary_section += "
    \n" } } - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + def yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" as String yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" @@ -161,7 +160,7 @@ def paramsSummaryMultiqc(summary_params) { // nf-core logo // def nfCoreLogo(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map String.format( """\n ${dashedLine(monochrome_logs)} @@ -180,7 +179,7 @@ def nfCoreLogo(monochrome_logs=true) { // Return dashed line // def dashedLine(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map return "-${colors.dim}----------------------------------------------------${colors.reset}-" } @@ -188,7 +187,7 @@ def dashedLine(monochrome_logs=true) { // ANSII colours used for terminal logging // def logColours(monochrome_logs=true) { - Map colorcodes = [:] + def colorcodes = [:] as Map // Reset / Meta colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" @@ -287,7 +286,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi } def summary = [:] - for (group in summary_params.keySet()) { + summary_params.keySet().sort().each { group -> summary << summary_params[group] } @@ -344,10 +343,10 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi def sendmail_html = sendmail_template.toString() // Send the HTML e-mail - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map if (email_address) { try { - if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + if (plaintext_email) { throw new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") sendmail_tf.withWriter { w -> w << sendmail_html } @@ -364,13 +363,13 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Write summary e-mail HTML to a file def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); output_hf.delete() // Write summary e-mail TXT to a file def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); output_tf.delete() } @@ -378,7 +377,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Print pipeline summary on completion // def completionSummary(monochrome_logs=true) { - Map colors = logColours(monochrome_logs) + def colors = logColours(monochrome_logs) as Map if (workflow.success) { if (workflow.stats.ignoredCount == 0) { log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" @@ -395,7 +394,7 @@ def completionSummary(monochrome_logs=true) { // def imNotification(summary_params, hook_url) { def summary = [:] - for (group in summary_params.keySet()) { + summary_params.keySet().sort().each { group -> summary << summary_params[group] } diff --git a/subworkflows/nf-core/utils_nfschema_plugin/main.nf b/subworkflows/nf-core/utils_nfschema_plugin/main.nf new file mode 100644 index 0000000..4994303 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/main.nf @@ -0,0 +1,46 @@ +// +// Subworkflow that uses the nf-schema plugin to validate parameters and render the parameter summary +// + +include { paramsSummaryLog } from 'plugin/nf-schema' +include { validateParameters } from 'plugin/nf-schema' + +workflow UTILS_NFSCHEMA_PLUGIN { + + take: + input_workflow // workflow: the workflow object used by nf-schema to get metadata from the workflow + validate_params // boolean: validate the parameters + parameters_schema // string: path to the parameters JSON schema. + // this has to be the same as the schema given to `validation.parametersSchema` + // when this input is empty it will automatically use the configured schema or + // "${projectDir}/nextflow_schema.json" as default. This input should not be empty + // for meta pipelines + + main: + + // + // Print parameter summary to stdout. This will display the parameters + // that differ from the default given in the JSON schema + // + if(parameters_schema) { + log.info paramsSummaryLog(input_workflow, parameters_schema:parameters_schema) + } else { + log.info paramsSummaryLog(input_workflow) + } + + // + // Validate the parameters using nextflow_schema.json or the schema + // given via the validation.parametersSchema configuration option + // + if(validate_params) { + if(parameters_schema) { + validateParameters(parameters_schema:parameters_schema) + } else { + validateParameters() + } + } + + emit: + dummy_emit = true +} + diff --git a/subworkflows/nf-core/utils_nfschema_plugin/meta.yml b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml new file mode 100644 index 0000000..f7d9f02 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/meta.yml @@ -0,0 +1,35 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "utils_nfschema_plugin" +description: Run nf-schema to validate parameters and create a summary of changed parameters +keywords: + - validation + - JSON schema + - plugin + - parameters + - summary +components: [] +input: + - input_workflow: + type: object + description: | + The workflow object of the used pipeline. + This object contains meta data used to create the params summary log + - validate_params: + type: boolean + description: Validate the parameters and error if invalid. + - parameters_schema: + type: string + description: | + Path to the parameters JSON schema. + This has to be the same as the schema given to the `validation.parametersSchema` config + option. When this input is empty it will automatically use the configured schema or + "${projectDir}/nextflow_schema.json" as default. The schema should not be given in this way + for meta pipelines. +output: + - dummy_emit: + type: boolean + description: Dummy emit to make nf-core subworkflows lint happy +authors: + - "@nvnieuwk" +maintainers: + - "@nvnieuwk" diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test new file mode 100644 index 0000000..842dc43 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test @@ -0,0 +1,117 @@ +nextflow_workflow { + + name "Test Subworkflow UTILS_NFSCHEMA_PLUGIN" + script "../main.nf" + workflow "UTILS_NFSCHEMA_PLUGIN" + + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/utils_nfschema_plugin" + tag "plugin/nf-schema" + + config "./nextflow.config" + + test("Should run nothing") { + + when { + + params { + test_data = '' + } + + workflow { + """ + validate_params = false + input[0] = workflow + input[1] = validate_params + input[2] = "" + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should validate params") { + + when { + + params { + test_data = '' + outdir = 1 + } + + workflow { + """ + validate_params = true + input[0] = workflow + input[1] = validate_params + input[2] = "" + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } } + ) + } + } + + test("Should run nothing - custom schema") { + + when { + + params { + test_data = '' + } + + workflow { + """ + validate_params = false + input[0] = workflow + input[1] = validate_params + input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should validate params - custom schema") { + + when { + + params { + test_data = '' + outdir = 1 + } + + workflow { + """ + validate_params = true + input[0] = workflow + input[1] = validate_params + input[2] = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config new file mode 100644 index 0000000..0907ac5 --- /dev/null +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow.config @@ -0,0 +1,8 @@ +plugins { + id "nf-schema@2.1.0" +} + +validation { + parametersSchema = "${projectDir}/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json" + monochromeLogs = true +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json similarity index 95% rename from subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json rename to subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json index 7626c1c..331e0d2 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/nextflow_schema.json @@ -1,10 +1,10 @@ { - "$schema": "http://json-schema.org/draft-07/schema", + "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", "title": ". pipeline parameters", "description": "", "type": "object", - "definitions": { + "$defs": { "input_output_options": { "title": "Input/output options", "type": "object", @@ -87,10 +87,10 @@ }, "allOf": [ { - "$ref": "#/definitions/input_output_options" + "$ref": "#/$defs/input_output_options" }, { - "$ref": "#/definitions/generic_options" + "$ref": "#/$defs/generic_options" } ] } diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf deleted file mode 100644 index 2585b65..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf +++ /dev/null @@ -1,62 +0,0 @@ -// -// Subworkflow that uses the nf-validation plugin to render help text and parameter summary -// - -/* -======================================================================================== - IMPORT NF-VALIDATION PLUGIN -======================================================================================== -*/ - -include { paramsHelp } from 'plugin/nf-validation' -include { paramsSummaryLog } from 'plugin/nf-validation' -include { validateParameters } from 'plugin/nf-validation' - -/* -======================================================================================== - SUBWORKFLOW DEFINITION -======================================================================================== -*/ - -workflow UTILS_NFVALIDATION_PLUGIN { - - take: - print_help // boolean: print help - workflow_command // string: default commmand used to run pipeline - pre_help_text // string: string to be printed before help text and summary log - post_help_text // string: string to be printed after help text and summary log - validate_params // boolean: validate parameters - schema_filename // path: JSON schema file, null to use default value - - main: - - log.debug "Using schema file: ${schema_filename}" - - // Default values for strings - pre_help_text = pre_help_text ?: '' - post_help_text = post_help_text ?: '' - workflow_command = workflow_command ?: '' - - // - // Print help message if needed - // - if (print_help) { - log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text - System.exit(0) - } - - // - // Print parameter summary to stdout - // - log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text - - // - // Validate parameters relative to the parameter JSON schema - // - if (validate_params){ - validateParameters(parameters_schema: schema_filename) - } - - emit: - dummy_emit = true -} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml deleted file mode 100644 index 3d4a6b0..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml +++ /dev/null @@ -1,44 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json -name: "UTILS_NFVALIDATION_PLUGIN" -description: Use nf-validation to initiate and validate a pipeline -keywords: - - utility - - pipeline - - initialise - - validation -components: [] -input: - - print_help: - type: boolean - description: | - Print help message and exit - - workflow_command: - type: string - description: | - The command to run the workflow e.g. "nextflow run main.nf" - - pre_help_text: - type: string - description: | - Text to print before the help message - - post_help_text: - type: string - description: | - Text to print after the help message - - validate_params: - type: boolean - description: | - Validate the parameters and error if invalid. - - schema_filename: - type: string - description: | - The filename of the schema to validate against. -output: - - dummy_emit: - type: boolean - description: | - Dummy emit to make nf-core subworkflows lint happy -authors: - - "@adamrtalbot" -maintainers: - - "@adamrtalbot" - - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test deleted file mode 100644 index 5784a33..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ /dev/null @@ -1,200 +0,0 @@ -nextflow_workflow { - - name "Test Workflow UTILS_NFVALIDATION_PLUGIN" - script "../main.nf" - workflow "UTILS_NFVALIDATION_PLUGIN" - tag "subworkflows" - tag "subworkflows_nfcore" - tag "plugin/nf-validation" - tag "'plugin/nf-validation'" - tag "utils_nfvalidation_plugin" - tag "subworkflows/utils_nfvalidation_plugin" - - test("Should run nothing") { - - when { - - params { - monochrome_logs = true - test_data = '' - } - - workflow { - """ - help = false - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success } - ) - } - } - - test("Should run help") { - - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } } - ) - } - } - - test("Should run help with command") { - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = "nextflow run noorg/doesntexist" - pre_help_text = null - post_help_text = null - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } } - ) - } - } - - test("Should run help with extra text") { - - - when { - - params { - monochrome_logs = true - test_data = '' - } - workflow { - """ - help = true - workflow_command = "nextflow run noorg/doesntexist" - pre_help_text = "pre-help-text" - post_help_text = "post-help-text" - validate_params = false - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.success }, - { assert workflow.exitStatus == 0 }, - { assert workflow.stdout.any { it.contains('pre-help-text') } }, - { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, - { assert workflow.stdout.any { it.contains('Input/output options') } }, - { assert workflow.stdout.any { it.contains('--outdir') } }, - { assert workflow.stdout.any { it.contains('post-help-text') } } - ) - } - } - - test("Should validate params") { - - when { - - params { - monochrome_logs = true - test_data = '' - outdir = 1 - } - workflow { - """ - help = false - workflow_command = null - pre_help_text = null - post_help_text = null - validate_params = true - schema_filename = "$moduleTestDir/nextflow_schema.json" - - input[0] = help - input[1] = workflow_command - input[2] = pre_help_text - input[3] = post_help_text - input[4] = validate_params - input[5] = schema_filename - """ - } - } - - then { - assertAll( - { assert workflow.failed }, - { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } - ) - } - } -} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml deleted file mode 100644 index 60b1cff..0000000 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -subworkflows/utils_nfvalidation_plugin: - - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index eeba42a..2f01cfa 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -3,10 +3,9 @@ IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - include { FASTQC } from '../modules/nf-core/fastqc/main' include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMap } from 'plugin/nf-schema' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' @@ -21,12 +20,10 @@ workflow VARIANTBENCHMARKING { take: ch_samplesheet // channel: samplesheet read in from --input - main: ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() - // // MODULE: Run FastQC // @@ -42,11 +39,12 @@ workflow VARIANTBENCHMARKING { softwareVersionsToYAML(ch_versions) .collectFile( storeDir: "${params.outdir}/pipeline_info", - name: 'nf_core_pipeline_software_mqc_versions.yml', + name: 'nf_core_' + 'pipeline_software_' + 'mqc_' + 'versions.yml', sort: true, newLine: true ).set { ch_collated_versions } + // // MODULE: MultiQC // @@ -59,18 +57,19 @@ workflow VARIANTBENCHMARKING { Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap( workflow, parameters_schema: "nextflow_schema.json") ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - + ch_multiqc_files = ch_multiqc_files.mix( + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) ch_methods_description = Channel.value( methodsDescriptionText(ch_multiqc_custom_methods_description)) - ch_multiqc_files = ch_multiqc_files.mix( - ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) ch_multiqc_files = ch_multiqc_files.mix( ch_methods_description.collectFile( @@ -83,12 +82,14 @@ workflow VARIANTBENCHMARKING { ch_multiqc_files.collect(), ch_multiqc_config.toList(), ch_multiqc_custom_config.toList(), - ch_multiqc_logo.toList() + ch_multiqc_logo.toList(), + [], + [] ) - emit: - multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + emit:multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html versions = ch_versions // channel: [ path(versions.yml) ] + } /* From 59840c90a7d6f570ab507036ee2a39df2d14c741 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 9 Oct 2024 11:09:16 +0000 Subject: [PATCH 179/292] Template update for nf-core/tools version 3.0.1 --- .editorconfig | 4 - .github/CONTRIBUTING.md | 2 +- .github/workflows/awsfulltest.yml | 6 +- .github/workflows/linting.yml | 4 +- .nf-core.yml | 2 +- .prettierignore | 1 - docs/output.md | 1 - modules.json | 6 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 26 +- nextflow.config | 8 +- .../main.nf | 12 +- .../nf-core/utils_nextflow_pipeline/main.nf | 46 ++- .../nf-core/utils_nfcore_pipeline/main.nf | 279 ++++++++++-------- 15 files changed, 209 insertions(+), 194 deletions(-) diff --git a/.editorconfig b/.editorconfig index e105881..72dda28 100644 --- a/.editorconfig +++ b/.editorconfig @@ -11,7 +11,6 @@ indent_style = space [*.{md,yml,yaml,html,css,scss,js}] indent_size = 2 - # These files are edited and tested upstream in nf-core/modules [/modules/nf-core/**] charset = unset @@ -26,12 +25,9 @@ insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset - - [/assets/email*] indent_size = unset - # ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 2f2ce78..37f8c98 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -90,7 +90,7 @@ Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json ### Default processes resource requirements -Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. +Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/main/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 9c77a26..6289350 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,16 +14,18 @@ on: jobs: run-platform: name: Run AWS full tests - if: github.repository == 'nf-core/variantbenchmarking' && github.event.review.state == 'approved' + # run only if the PR is approved by at least 2 reviewers and against the master branch or manually triggered + if: github.repository == 'nf-core/variantbenchmarking' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch' runs-on: ubuntu-latest steps: - uses: octokit/request-action@v2.x id: check_approvals with: - route: GET /repos/${{ github.repository }}/pulls/${{ github.event.review.number }}/reviews + route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews env: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} - id: test_variables + if: github.event_name != 'workflow_dispatch' run: | JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}' CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length') diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index b882838..a502573 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -42,10 +42,10 @@ jobs: architecture: "x64" - name: read .nf-core.yml - uses: pietrobolcato/action-read-yaml@1.0.0 + uses: pietrobolcato/action-read-yaml@1.1.0 id: read_yml with: - config: ${{ github.workspace }}/.nf-core.yaml + config: ${{ github.workspace }}/.nf-core.yml - name: Install dependencies run: | diff --git a/.nf-core.yml b/.nf-core.yml index 5124a75..36a5b95 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,6 @@ bump_version: null lint: null -nf_core_version: 3.0.0 +nf_core_version: 3.0.1 org_path: null repository_type: pipeline template: diff --git a/.prettierignore b/.prettierignore index 610e506..437d763 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,4 +1,3 @@ - email_template.html adaptivecard.json slackreport.json diff --git a/docs/output.md b/docs/output.md index 9ee97dd..eb14937 100644 --- a/docs/output.md +++ b/docs/output.md @@ -14,7 +14,6 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [FastQC](#fastqc) - Raw read QC - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ### FastQC diff --git a/modules.json b/modules.json index 550f632..c58adec 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "git_sha": "b8d36829fa84b6e404364abff787e8b07f6d058c", "installed_by": ["modules"] } } @@ -21,12 +21,12 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "d20fb2a9cc3e2835e9d067d1046a63252eb17352", + "git_sha": "9d05360da397692321d377b6102d2fb22507c6ef", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "2fdce49d30c0254f76bc0f13c55c17455c1251ab", + "git_sha": "772684d9d66f37b650c8ba5146ac1ee3ecba2acb", "installed_by": ["subworkflows"] }, "utils_nfschema_plugin": { diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index f1cd99b..6f5b867 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -2,4 +2,4 @@ channels: - conda-forge - bioconda dependencies: - - bioconda::multiqc=1.24.1 + - bioconda::multiqc=1.25.1 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index b9ccebd..9724d2f 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.25--pyhdfd78af_0' : - 'biocontainers/multiqc:1.25--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.25.1--pyhdfd78af_0' : + 'biocontainers/multiqc:1.25.1--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index b779e46..2fcbb5f 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd" + "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-10T12:41:34.562023" + "timestamp": "2024-10-02T17:51:46.317523" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd" + "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-10T11:27:11.933869532" + "timestamp": "2024-10-02T17:52:20.680978" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,8c8724363a5efe0c6f43ab34faa57efd" + "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" ] ], "meta": { - "nf-test": "0.8.4", - "nextflow": "24.04.2" + "nf-test": "0.9.0", + "nextflow": "24.04.4" }, - "timestamp": "2024-07-10T11:26:56.709849369" + "timestamp": "2024-10-02T17:52:09.185842" } -} +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 09ac17e..a8b79da 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,10 +12,12 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options input = null + // References genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false + // MultiQC options multiqc_config = null multiqc_title = null @@ -36,6 +38,7 @@ params { show_hidden = false version = false pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' + // Config options config_profile_name = null config_profile_description = null @@ -44,9 +47,9 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null + // Schema validation default options validate_params = true - } // Load base.config by default for all pipelines @@ -161,6 +164,7 @@ includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${pa // Load nf-core/variantbenchmarking custom profiles from different institutions. // TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs // includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/variantbenchmarking.config" : "/dev/null" + // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled // Set to your registry if you have a mirror of containers @@ -172,6 +176,7 @@ charliecloud.registry = 'quay.io' // Load igenomes.config if required includeConfig !params.igenomes_ignore ? 'conf/igenomes.config' : 'conf/igenomes_ignored.config' + // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. @@ -263,4 +268,3 @@ validation { // Load modules.config for DSL2 module specific options includeConfig 'conf/modules.config' - diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index 08a15f4..dfe900c 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -18,9 +18,9 @@ include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW TO INITIALISE PIPELINE -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow PIPELINE_INITIALISATION { @@ -99,9 +99,9 @@ workflow PIPELINE_INITIALISATION { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW FOR PIPELINE COMPLETION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow PIPELINE_COMPLETION { @@ -147,9 +147,9 @@ workflow PIPELINE_COMPLETION { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // // Check and validate pipeline parameters diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index 28e32b2..2b0dc67 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -3,13 +3,12 @@ // /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NEXTFLOW_PIPELINE { - take: print_version // boolean: print version dump_parameters // boolean: dump parameters @@ -22,7 +21,7 @@ workflow UTILS_NEXTFLOW_PIPELINE { // Print workflow version and exit on --version // if (print_version) { - log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + log.info("${workflow.manifest.name} ${getWorkflowVersion()}") System.exit(0) } @@ -45,9 +44,9 @@ workflow UTILS_NEXTFLOW_PIPELINE { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // @@ -72,11 +71,11 @@ def getWorkflowVersion() { // Dump pipeline parameters to a JSON file // def dumpParametersToJSON(outdir) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = groovy.json.JsonOutput.toJson(params) - temp_pf.text = groovy.json.JsonOutput.prettyPrint(jsonStr) + def timestamp = new java.util.Date().format('yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = groovy.json.JsonOutput.toJson(params) + temp_pf.text = groovy.json.JsonOutput.prettyPrint(jsonStr) nextflow.extension.FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") temp_pf.delete() @@ -91,9 +90,14 @@ def checkCondaChannels() { try { def config = parser.load("conda config --show channels".execute().text) channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return + } + catch (NullPointerException e) { + log.warn("Could not verify conda channel configuration.") + return null + } + catch (IOException e) { + log.warn("Could not verify conda channel configuration.") + return null } // Check that all channels are present @@ -106,19 +110,13 @@ def checkCondaChannels() { required_channels_in_order.eachWithIndex { channel, index -> if (index < required_channels_in_order.size() - 1) { - channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index+1])) + channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index + 1])) } } if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + log.warn( + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + " There is a problem with your Conda configuration!\n\n" + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + " Please refer to https://bioconda.github.io/\n" + " The observed channel order is \n" + " ${channels}\n" + " but the following channel order is required:\n" + " ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + ) } } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index cbd8495..b78273c 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -3,13 +3,12 @@ // /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NFCORE_PIPELINE { - take: nextflow_cli_args @@ -22,9 +21,9 @@ workflow UTILS_NFCORE_PIPELINE { } /* -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -======================================================================================== +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // @@ -33,12 +32,9 @@ workflow UTILS_NFCORE_PIPELINE { def checkConfigProvided() { def valid_config = true as Boolean if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " + log.warn( + "[${workflow.manifest.name}] You are attempting to run the pipeline without any custom configuration!\n\n" + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + "Please refer to the quick start section and usage docs for the pipeline.\n " + ) valid_config = false } return valid_config @@ -49,12 +45,14 @@ def checkConfigProvided() { // def checkProfileProvided(nextflow_cli_args) { if (workflow.profile.endsWith(',')) { - error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + - "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + error( + "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + ) } if (nextflow_cli_args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + - "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + log.warn( + "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + ) } } @@ -70,13 +68,7 @@ def workflowCitation() { manifest_doi.each { doi_ref -> temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" } - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - temp_doi_ref + "\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } // @@ -102,7 +94,7 @@ def getWorkflowVersion() { // def processVersionsFromYAML(yaml_file) { def yaml = new org.yaml.snakeyaml.Yaml() - def versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + def versions = yaml.load(yaml_file).collectEntries { k, v -> [k.tokenize(':')[-1], v] } return yaml.dumpAsMap(versions).trim() } @@ -112,8 +104,8 @@ def processVersionsFromYAML(yaml_file) { def workflowVersionToYAML() { return """ Workflow: - $workflow.manifest.name: ${getWorkflowVersion()} - Nextflow: $workflow.nextflow.version + ${workflow.manifest.name}: ${getWorkflowVersion()} + Nextflow: ${workflow.nextflow.version} """.stripIndent().trim() } @@ -121,11 +113,7 @@ def workflowVersionToYAML() { // Get channel of software versions used in pipeline in YAML format // def softwareVersionsToYAML(ch_versions) { - return ch_versions - .unique() - .map { version -> processVersionsFromYAML(version) } - .unique() - .mix(Channel.of(workflowVersionToYAML())) + return ch_versions.unique().map { version -> processVersionsFromYAML(version) }.unique().mix(Channel.of(workflowVersionToYAML())) } // @@ -133,25 +121,31 @@ def softwareVersionsToYAML(ch_versions) { // def paramsSummaryMultiqc(summary_params) { def summary_section = '' - summary_params.keySet().each { group -> - def group_params = summary_params.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - group_params.keySet().sort().each { param -> - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + summary_params + .keySet() + .each { group -> + def group_params = summary_params.get(group) + // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    ${group}

    \n" + summary_section += "
    \n" + group_params + .keySet() + .sort() + .each { param -> + summary_section += "
    ${param}
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" } - summary_section += "
    \n" } - } - def yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" as String - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" + def yaml_file_text = "id: '${workflow.manifest.name.replace('/', '-')}-summary'\n" as String + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" return yaml_file_text } @@ -199,54 +193,54 @@ def logColours(monochrome_logs=true) { colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" return colorcodes } @@ -261,14 +255,15 @@ def attachMultiqcReport(multiqc_report) { mqc_report = multiqc_report.getVal() if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one") } mqc_report = mqc_report[0] } } - } catch (all) { + } + catch (Exception all) { if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email") } } return mqc_report @@ -280,26 +275,35 @@ def attachMultiqcReport(multiqc_report) { def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + def subject = "[${workflow.manifest.name}] Successful: ${workflow.runName}" if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + subject = "[${workflow.manifest.name}] FAILED: ${workflow.runName}" } def summary = [:] - summary_params.keySet().sort().each { group -> - summary << summary_params[group] - } + summary_params + .keySet() + .sort() + .each { group -> + summary << summary_params[group] + } def misc_fields = [:] misc_fields['Date Started'] = workflow.start misc_fields['Date Completed'] = workflow.complete misc_fields['Pipeline script file path'] = workflow.scriptFile misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build + if (workflow.repository) { + misc_fields['Pipeline repository Git URL'] = workflow.repository + } + if (workflow.commitId) { + misc_fields['Pipeline repository Git Commit'] = workflow.commitId + } + if (workflow.revision) { + misc_fields['Pipeline Git branch/tag'] = workflow.revision + } + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp def email_fields = [:] @@ -337,7 +341,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi // Render the sendmail template def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def smail_fields = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) def sendmail_html = sendmail_template.toString() @@ -346,30 +350,32 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi def colors = logColours(monochrome_logs) as Map if (email_address) { try { - if (plaintext_email) { throw new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } + if (plaintext_email) { +new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { + ['sendmail', '-t'].execute() << sendmail_html + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-") + } + catch (Exception all) { // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address] mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (mail)-") } } // Write summary e-mail HTML to a file def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + nextflow.extension.FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html") output_hf.delete() // Write summary e-mail TXT to a file def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } - nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + nextflow.extension.FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt") output_tf.delete() } @@ -380,12 +386,14 @@ def completionSummary(monochrome_logs=true) { def colors = logColours(monochrome_logs) as Map if (workflow.success) { if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Pipeline completed successfully${colors.reset}-") + } + else { + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-") } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } + else { + log.info("-${colors.purple}[${workflow.manifest.name}]${colors.red} Pipeline completed with errors${colors.reset}-") } } @@ -394,21 +402,30 @@ def completionSummary(monochrome_logs=true) { // def imNotification(summary_params, hook_url) { def summary = [:] - summary_params.keySet().sort().each { group -> - summary << summary_params[group] - } + summary_params + .keySet() + .sort() + .each { group -> + summary << summary_params[group] + } def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) { + misc_fields['repository'] = workflow.repository + } + if (workflow.commitId) { + misc_fields['commitid'] = workflow.commitId + } + if (workflow.revision) { + misc_fields['revision'] = workflow.revision + } + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp def msg_fields = [:] msg_fields['version'] = getWorkflowVersion() @@ -433,13 +450,13 @@ def imNotification(summary_params, hook_url) { def json_message = json_template.toString() // POST - def post = new URL(hook_url).openConnection(); + def post = new URL(hook_url).openConnection() post.setRequestMethod("POST") post.setDoOutput(true) post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); + post.getOutputStream().write(json_message.getBytes("UTF-8")) + def postRC = post.getResponseCode() + if (!postRC.equals(200)) { + log.warn(post.getErrorStream().getText()) } } From 088ec123fd283e92882398d762915f3766a21fde Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 10 Oct 2024 14:33:46 +0200 Subject: [PATCH 180/292] correct padding --- nextflow.config | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index ffae447..8736a95 100644 --- a/nextflow.config +++ b/nextflow.config @@ -296,10 +296,10 @@ validation { """ afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { " https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""} * The nf-core framework - https://doi.org/10.1038/s41587-020-0439-x +https://doi.org/10.1038/s41587-020-0439-x * Software dependencies - https://github.com/${manifest.name}/blob/master/CITATIONS.md +https://github.com/${manifest.name}/blob/master/CITATIONS.md """ } summary { From aee082b5d9eb09de7ff66a3002e974064c01572c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 10 Oct 2024 14:43:56 +0200 Subject: [PATCH 181/292] fix samplesheet reading --- .../main.nf | 18 +++++++++++++++++- 1 file changed, 17 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index 59489f0..c820ce9 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -74,7 +74,23 @@ workflow PIPELINE_INITIALISATION { // Channel - .fromSamplesheet("input") + .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json")) + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { samplesheet -> + validateInputSamplesheet(samplesheet) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } .set { ch_samplesheet } emit: From abf69162c2c1bd77f76ccea821052ac94862d88f Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 10 Oct 2024 16:04:19 +0200 Subject: [PATCH 182/292] update bcftools filter and fix samplesheet input read --- assets/schema_input.json | 51 ++- modules.json | 2 +- .../nf-core/bcftools/filter/environment.yml | 4 +- modules/nf-core/bcftools/filter/main.nf | 20 +- modules/nf-core/bcftools/filter/meta.yml | 67 +++- .../bcftools/filter/tests/main.nf.test | 217 ++++++++++- .../bcftools/filter/tests/main.nf.test.snap | 363 +++++++++++++++++- .../filter/tests/region_filter.config | 4 + .../bcftools/filter/tests/vcf_gz_index.config | 4 + .../filter/tests/vcf_gz_index_csi.config | 4 + .../filter/tests/vcf_gz_index_tbi.config | 4 + nextflow_schema.json | 8 + .../main.nf | 32 +- workflows/variantbenchmarking.nf | 1 + 14 files changed, 684 insertions(+), 97 deletions(-) create mode 100644 modules/nf-core/bcftools/filter/tests/region_filter.config create mode 100644 modules/nf-core/bcftools/filter/tests/vcf_gz_index.config create mode 100644 modules/nf-core/bcftools/filter/tests/vcf_gz_index_csi.config create mode 100644 modules/nf-core/bcftools/filter/tests/vcf_gz_index_tbi.config diff --git a/assets/schema_input.json b/assets/schema_input.json index ba985aa..fd84111 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -45,107 +45,124 @@ "pattern": "^\\S+$", "description": "Name to subsample in the multisample VCF, might be required for somatic cases", "meta": ["subsample"], - "minLength": 1 + "minLength": 1, + "default": null }, "normshift": { "type": "number", "description": "normshift is a svbenchmark parameter. Has to be between 0-1. Disallow matches if alignments between alternate alleles have normalized shift greater than normshift (default 0.2)", "meta": ["normshift"], "minimum": 0, - "maximum": 1 + "maximum": 1, + "default": null }, "normdist": { "type": "number", "description": "normshift is a svbenchmark parameter. Has to be between 0-1. Disallow matches if alternate alleles have normalized edit distance greater than normdist (default 0.2)", "meta": ["normdist"], "minimum": 0, - "maximum": 1 + "maximum": 1, + "default": null }, "normsizediff": { "type": "number", "description": "normsizediff is a svbenchmark parameter. Has to be between 0-1. Disallow matches if alternate alleles have normalized size difference greater than normsizediff (default 0.2)", "meta": ["normsizediff"], "minimum": 0, - "maximum": 1 + "maximum": 1, + "default": null }, "maxdist": { "type": "integer", "description": "maxdist is a svbenchmark parameter. Disallow matches if positions of two variants are more than maxdist bases from each other (default 100,000).", "minimum": 0, - "meta": ["maxdist"] + "meta": ["maxdist"], + "default": null }, "pctsize": { "type": "number", "description": "pctsize is a truvari parameter. Has to be between 0-1. Ratio of min(base_size, comp_size)/max(base_size, comp_size).", "meta": ["pctsize"], "minimum": 0, - "maximum": 1 + "maximum": 1, + "default": null }, "pctseq": { "type": "number", "description": "pctseq is a truvari parameter. Has to be between 0-1. Edit distance ratio between the REF/ALT haplotype sequences of base and comparison call. turn it off (0) for no sequence comparison.", "meta": ["pctseq"], "minimum": 0, - "maximum": 1 + "maximum": 1, + "default": null }, "pctovl": { "type": "number", "description": "pctovl is a truvari parameter. Has to be between 0-1. Ratio of two calls' (overlapping bases)/(longest span)", "meta": ["pctovl"], "minimum": 0, - "maximum": 1 + "maximum": 1, + "default": null }, "refdist": { "type": "integer", "description": "refdist is a truvari parameter. Maximum distance comparison calls must be within from base call's start/end ", "meta": ["refdist"], - "minimum": 0 + "minimum": 0, + "default": null }, "chunksize": { "type": "integer", "description": "chunksize is a truvari parameter. Create chunks of all calls overlapping within ±`--chunksize` basepairs", "meta": ["chunksize"], - "minimum": 0 + "minimum": 0, + "default": null }, "dup_to_ins": { "type": "boolean", "description": "a truvari parameter. converts DUP to INS type", - "meta": ["dup_to_ins"] + "meta": ["dup_to_ins"], + "default": null }, "typeignore": { "type": "boolean", "description": "a truvari parameter. Ignore SVTYPE matching", - "meta": ["typeignore"] + "meta": ["typeignore"], + "default": null }, "bpDistance": { "type": "integer", "description": "bpDistance is a wittyer parameter. Upper bound of boundary distance when comparing truth and query. By default it is 500bp for all types except for Insertions, which are 100bp.Please note that if you set this value in the command line, it overrides all the defaults, so Insertions and other types will have the same bpd.", "meta": ["bpDistance"], - "minimum": 0 + "minimum": 0, + "default": null }, "percentThreshold": { "type": "number", "description": "percentThreshold is a wittyer parameter. This is used for percentage thresholding. For CopyNumberTandemRepeats, this determines how large of a RepeatUnitCount (RUC) threshold to use for large tandem repeats. For all other SVs, in order to match between query and truth, the distance between boundaries should be within a number thats proportional to total SV (default 0.25)", "meta": ["percentThreshold"], - "minimum": 0 + "minimum": 0, + "default": null }, "absoluteThreshold": { "type": "integer", "description": "absoluteThreshold is a wittyer parameter. This is used for absolute thresholding. For CopyNumberTandemRepeats, this determines how large of a RepeatUnitCount (RUC) threshold to use. For all other SVs, this is the upper bound of boundary distance when comparing truth and query. (default 10000)", "meta": ["absoluteThreshold"], - "minimum": 0 + "minimum": 0, + "default": null }, "maxMatches": { "type": "integer", "description": "maxMatches is a wittyer parameter. This is used for matching behaviour. Negative value means to match any number (for large SVs it is not recommended).", - "meta": ["maxMatches"] + "meta": ["maxMatches"], + "default": null }, "evaluationmode": { "type": "string", "description": "evaluationmode is a wittyer parameter. It is by default requires genotype matching. simpleCounting:sc, CrossTypeAndSimpleCounting:cts, genotypematch:d ", "meta": ["evaluationmode"], "enum": ["sc", "cts", "d"], - "minLength": 1 + "minLength": 1, + "default": null } }, "required": ["test_vcf", "caller", "vartype", "id"] diff --git a/modules.json b/modules.json index 72fea1b..52b8658 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "bcftools/filter": { "branch": "master", - "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "git_sha": "f85dbddd7a335fc0f5ac331e8d22ca94123b654b", "installed_by": ["modules"] }, "bcftools/merge": { diff --git a/modules/nf-core/bcftools/filter/environment.yml b/modules/nf-core/bcftools/filter/environment.yml index b269875..5c00b11 100644 --- a/modules/nf-core/bcftools/filter/environment.yml +++ b/modules/nf-core/bcftools/filter/environment.yml @@ -1,7 +1,5 @@ -name: bcftools_filter channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::bcftools=1.18 + - bioconda::bcftools=1.20 diff --git a/modules/nf-core/bcftools/filter/main.nf b/modules/nf-core/bcftools/filter/main.nf index cc9a236..36cbf8c 100644 --- a/modules/nf-core/bcftools/filter/main.nf +++ b/modules/nf-core/bcftools/filter/main.nf @@ -4,14 +4,16 @@ process BCFTOOLS_FILTER { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'biocontainers/bcftools:1.18--h8b25389_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.20--h8b25389_0': + 'biocontainers/bcftools:1.20--h8b25389_0' }" input: - tuple val(meta), path(vcf) + tuple val(meta), path(vcf), path(tbi) output: tuple val(meta), path("*.${extension}"), emit: vcf + tuple val(meta), path("*.tbi") , emit: tbi, optional: true + tuple val(meta), path("*.csi") , emit: csi, optional: true path "versions.yml" , emit: versions when: @@ -45,19 +47,23 @@ process BCFTOOLS_FILTER { stub: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - extension = args.contains("--output-type b") || args.contains("-Ob") ? "bcf.gz" : args.contains("--output-type u") || args.contains("-Ou") ? "bcf" : args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf" + def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" : + args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" : + args.contains("--write-index") || args.contains("-W") ? "csi" : + "" + def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch" + def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : "" if ("$vcf" == "${prefix}.${extension}") error "Input and output names are the same, set prefix in module configuration to disambiguate!" - def create_file = extension.endsWith(".gz") ? "echo '' | gzip > ${prefix}.${extension}" : "touch ${prefix}.${extension}" - """ - ${create_file} + ${create_cmd} ${prefix}.${extension} + ${create_index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bcftools/filter/meta.yml b/modules/nf-core/bcftools/filter/meta.yml index deb93b1..d72f275 100644 --- a/modules/nf-core/bcftools/filter/meta.yml +++ b/modules/nf-core/bcftools/filter/meta.yml @@ -12,30 +12,57 @@ tools: documentation: http://www.htslib.org/doc/bcftools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] + identifier: biotools:bcftools input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: VCF input file - pattern: "*.{vcf,bcf,vcf.gz,bcf.gz}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF input file + pattern: "*.{vcf,bcf,vcf.gz,bcf.gz}" + - tbi: + type: file + description: VCF index file + pattern: "*.tbi" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - vcf: - type: file - description: VCF filtered output file - pattern: "*.{vcf,bcf,vcf.gz,bcf.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.${extension}": + type: file + description: VCF filtered output file + pattern: "*.{vcf,bcf,vcf.gz,bcf.gz}" + - tbi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.tbi": + type: file + description: Alternative VCF file index + pattern: "*.tbi" + - csi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.csi": + type: file + description: Default VCF file index + pattern: "*.csi" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@joseespinosa" - "@drpatelh" diff --git a/modules/nf-core/bcftools/filter/tests/main.nf.test b/modules/nf-core/bcftools/filter/tests/main.nf.test index eaf100e..fadff0e 100644 --- a/modules/nf-core/bcftools/filter/tests/main.nf.test +++ b/modules/nf-core/bcftools/filter/tests/main.nf.test @@ -9,16 +9,17 @@ nextflow_process { tag "bcftools" tag "bcftools/filter" - config "./nextflow.config" - test("sarscov2 - vcf") { + config "./nextflow.config" + when { process { """ input[0] = [ [id:"vcf_test"], - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] ] """ } @@ -33,14 +34,110 @@ nextflow_process { } + test("sarscov2 - vcf_gz_index") { + + config "./vcf_gz_index.config" + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_csi") { + + config "./vcf_gz_index_csi.config" + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_tbi") { + + config "./vcf_gz_index_tbi.config" + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.tbi[0][1].endsWith(".tbi") } + ) + } + + } + test("sarscov2 - vcf - bcf output") { + config "./nextflow.config" + when { process { """ input[0] = [ [id:"bcf_test"], - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] ] """ } @@ -55,8 +152,34 @@ nextflow_process { } + test("sarscov2 - vcf.gz, tbi - region filter") { + + config "./region_filter.config" + + when { + process { + """ + input[0] = [ + [id:"bcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("region filter") } + ) + } + + } + test("sarscov2 - vcf - stub") { + config "./nextflow.config" options "-stub" when { @@ -64,7 +187,8 @@ nextflow_process { """ input[0] = [ [id:"vcf_test"], - file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] ] """ } @@ -79,4 +203,85 @@ nextflow_process { } -} + test("sarscov2 - vcf_gz_index - stub") { + + config "./vcf_gz_index.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_csi - stub") { + + config "./vcf_gz_index_csi.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_tbi - stub") { + + config "./vcf_gz_index_tbi.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [id:"vcf_test"], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), + [] + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.tbi[0][1].endsWith(".tbi") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/filter/tests/main.nf.test.snap b/modules/nf-core/bcftools/filter/tests/main.nf.test.snap index f8e17aa..640907e 100644 --- a/modules/nf-core/bcftools/filter/tests/main.nf.test.snap +++ b/modules/nf-core/bcftools/filter/tests/main.nf.test.snap @@ -1,4 +1,104 @@ { + "region filter": { + "content": [ + { + "0": [ + [ + { + "id": "bcf_test" + }, + "bcf_test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ], + "csi": [ + + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "bcf_test" + }, + "bcf_test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + "versions": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-08T09:14:47.394005264" + }, + "sarscov2 - vcf_gz_index_tbi - stub": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T13:59:08.235854993" + }, "vcf": { "content": [ { @@ -11,7 +111,19 @@ ] ], "1": [ - "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ], + "csi": [ + + ], + "tbi": [ + ], "vcf": [ [ @@ -22,15 +134,15 @@ ] ], "versions": [ - "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" ] } ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.02.0" + "nextflow": "24.04.2" }, - "timestamp": "2024-03-27T16:57:32.940161987" + "timestamp": "2024-06-04T15:20:28.427974818" }, "bcf output": { "content": [ @@ -44,7 +156,19 @@ ] ], "1": [ - "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ], + "csi": [ + + ], + "tbi": [ + ], "vcf": [ [ @@ -55,15 +179,77 @@ ] ], "versions": [ - "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" ] } ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.02.0" + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-04T15:20:34.049236749" + }, + "sarscov2 - vcf_gz_index": { + "content": [ + [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.csi" + ] + ], + [ + + ], + [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T08:09:49.837854646" + }, + "sarscov2 - vcf_gz_index_csi": { + "content": [ + [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.csi" + ] + ], + [ + + ], + [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" }, - "timestamp": "2024-03-27T16:45:14.586866398" + "timestamp": "2024-06-05T13:57:19.513365022" }, "vcf - stub": { "content": [ @@ -77,7 +263,19 @@ ] ], "1": [ - "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ], + "csi": [ + + ], + "tbi": [ + ], "vcf": [ [ @@ -88,14 +286,155 @@ ] ], "versions": [ - "versions.yml:md5,7dc77043f9afb848d942d47a7bc19f67" + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-04T15:29:55.846566153" + }, + "sarscov2 - vcf_gz_index - stub": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ], + "csi": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-04T15:59:37.636874258" + }, + "sarscov2 - vcf_gz_index_csi - stub": { + "content": [ + { + "0": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ], + "csi": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" ] } ], "meta": { "nf-test": "0.8.4", - "nextflow": "24.02.0" + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T13:58:46.36278584" + }, + "sarscov2 - vcf_gz_index_tbi": { + "content": [ + [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + + ], + [ + [ + { + "id": "vcf_test" + }, + "vcf_test_vcf.vcf.gz.tbi" + ] + ], + [ + "versions.yml:md5,9a336d1ee26b527d7a2bdbeead155f64" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" }, - "timestamp": "2024-03-27T17:05:52.80837892" + "timestamp": "2024-06-05T13:57:34.748836577" } } \ No newline at end of file diff --git a/modules/nf-core/bcftools/filter/tests/region_filter.config b/modules/nf-core/bcftools/filter/tests/region_filter.config new file mode 100644 index 0000000..b18fb4b --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/region_filter.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z -r MT192765.1 --no-version" +} diff --git a/modules/nf-core/bcftools/filter/tests/vcf_gz_index.config b/modules/nf-core/bcftools/filter/tests/vcf_gz_index.config new file mode 100644 index 0000000..7dd696e --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/vcf_gz_index.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index --no-version" +} diff --git a/modules/nf-core/bcftools/filter/tests/vcf_gz_index_csi.config b/modules/nf-core/bcftools/filter/tests/vcf_gz_index_csi.config new file mode 100644 index 0000000..aebffb6 --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/vcf_gz_index_csi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=csi --no-version" +} diff --git a/modules/nf-core/bcftools/filter/tests/vcf_gz_index_tbi.config b/modules/nf-core/bcftools/filter/tests/vcf_gz_index_tbi.config new file mode 100644 index 0000000..b192ae7 --- /dev/null +++ b/modules/nf-core/bcftools/filter/tests/vcf_gz_index_tbi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=tbi --no-version" +} diff --git a/nextflow_schema.json b/nextflow_schema.json index 290159c..51ed763 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -250,6 +250,14 @@ "hidden": true, "help_text": "Do not load `igenomes.config` when running the pipeline. You may choose this option if you observe clashes between custom parameters and those supplied in `igenomes.config`." }, + "igenomes_base": { + "type": "string", + "format": "directory-path", + "description": "The base path to the igenomes reference files", + "fa_icon": "fas fa-ban", + "hidden": true, + "default": "s3://ngi-igenomes/igenomes/" + }, "itruth_ignore": { "type": "boolean", "description": "Do not load the truth reference config.", diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index c820ce9..56e2038 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -74,23 +74,7 @@ workflow PIPELINE_INITIALISATION { // Channel - .fromList(samplesheetToList(params.input, "${projectDir}/assets/schema_input.json")) - .map { - meta, fastq_1, fastq_2 -> - if (!fastq_2) { - return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] - } else { - return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] - } - } - .groupTuple() - .map { samplesheet -> - validateInputSamplesheet(samplesheet) - } - .map { - meta, fastqs -> - return [ meta, fastqs.flatten() ] - } + .fromList(samplesheetToList(params.input,"${projectDir}/assets/schema_input.json")) .set { ch_samplesheet } emit: @@ -158,20 +142,6 @@ def validateInputParameters() { genomeExistsError() } -// -// Validate channels from input samplesheet -// -def validateInputSamplesheet(input) { - def (metas, fastqs) = input[1..2] - - // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end - def endedness_ok = metas.collect{ meta -> meta.single_end }.unique().size == 1 - if (!endedness_ok) { - error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") - } - - return [ metas[0], fastqs ] -} // // Get attribute from genome config file e.g. fasta // diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index e5fa6e4..e38b64f 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -145,6 +145,7 @@ workflow VARIANTBENCHMARKING { out_vcf_ch = Channel.empty() + input.multisample.view() SUBSAMPLE_VCF_TEST( input.multisample ) From 000a7f401a38165f8ba89749b9094c881e00825c Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 11 Oct 2024 12:35:11 +0000 Subject: [PATCH 183/292] Template update for nf-core/tools version 3.0.2 --- .github/workflows/ci.yml | 60 +++++++++++++------ .../workflows/template_version_comment.yml | 21 ++++--- .gitignore | 1 + .nf-core.yml | 2 +- main.nf | 2 +- modules.json | 6 +- modules/nf-core/multiqc/main.nf | 2 +- nextflow.config | 4 +- .../main.nf | 4 +- .../nf-core/utils_nextflow_pipeline/main.nf | 30 +++++----- .../nf-core/utils_nfcore_pipeline/main.nf | 10 ++-- workflows/variantbenchmarking.nf | 2 - 12 files changed, 86 insertions(+), 58 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 7656200..c599817 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -11,6 +11,8 @@ on: env: NXF_ANSI_LOG: false + NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity + NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity concurrency: group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" @@ -18,7 +20,7 @@ concurrency: jobs: test: - name: Run pipeline with test data + name: "Run pipeline with test data (${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }})" # Only run on push if this is the nf-core dev branch (merged PRs) if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/variantbenchmarking') }}" runs-on: ubuntu-latest @@ -27,33 +29,57 @@ jobs: NXF_VER: - "24.04.2" - "latest-everything" + profile: + - "conda" + - "docker" + - "singularity" + test_name: + - "test" + isMaster: + - ${{ github.base_ref == 'master' }} + # Exclude conda and singularity on dev + exclude: + - isMaster: false + profile: "conda" + - isMaster: false + profile: "singularity" steps: - name: Check out pipeline code uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - name: Install Nextflow + - name: Set up Nextflow uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" - - name: Disk space cleanup - uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Set up Apptainer + if: matrix.profile == 'singularity' + uses: eWaterCycle/setup-apptainer@main - - name: Run pipeline with test data (docker) - # TODO nf-core: You can customise CI pipeline run tests as required - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix + - name: Set up Singularity + if: matrix.profile == 'singularity' run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + mkdir -p $NXF_SINGULARITY_CACHEDIR + mkdir -p $NXF_SINGULARITY_LIBRARYDIR + + - name: Set up Miniconda + if: matrix.profile == 'conda' + uses: conda-incubator/setup-miniconda@a4260408e20b96e80095f42ff7f1a15b27dd94ca # v3 + with: + miniconda-version: "latest" + auto-update-conda: true + conda-solver: libmamba + channels: conda-forge,bioconda - - name: Run pipeline with test data (singularity) - # TODO nf-core: You can customise CI pipeline run tests as required + - name: Set up Conda + if: matrix.profile == 'conda' run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results - if: "${{ github.base_ref == 'master' }}" + echo $(realpath $CONDA)/condabin >> $GITHUB_PATH + echo $(realpath python) >> $GITHUB_PATH + + - name: Clean up Disk space + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - name: Run pipeline with test data (conda) - # TODO nf-core: You can customise CI pipeline run tests as required + - name: "Run pipeline with test data ${{ matrix.NXF_VER }} | ${{ matrix.test_name }} | ${{ matrix.profile }}" run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,conda --outdir ./results - if: "${{ github.base_ref == 'master' }}" + nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.test_name }},${{ matrix.profile }} --outdir ./results diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml index 9dea41f..e8aafe4 100644 --- a/.github/workflows/template_version_comment.yml +++ b/.github/workflows/template_version_comment.yml @@ -10,9 +10,11 @@ jobs: steps: - name: Check out pipeline code uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + with: + ref: ${{ github.event.pull_request.head.sha }} - name: Read template version from .nf-core.yml - uses: pietrobolcato/action-read-yaml@1.0.0 + uses: nichmor/minimal-read-yaml@v0.0.2 id: read_yml with: config: ${{ github.workspace }}/.nf-core.yml @@ -24,20 +26,21 @@ jobs: - name: Check nf-core outdated id: nf_core_outdated - run: pip list --outdated | grep nf-core + run: echo "OUTPUT=$(pip list --outdated | grep nf-core)" >> ${GITHUB_ENV} - name: Post nf-core template version comment uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 if: | - ${{ steps.nf_core_outdated.outputs.stdout }} =~ 'nf-core' + contains(env.OUTPUT, 'nf-core') with: repo-token: ${{ secrets.NF_CORE_BOT_AUTH_TOKEN }} allow-repeats: false message: | - ## :warning: Newer version of the nf-core template is available. - - Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}. - Please update your pipeline to the latest version. - - For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync). + > [!WARNING] + > Newer version of the nf-core template is available. + > + > Your pipeline is using an old version of the nf-core template: ${{ steps.read_yml.outputs['nf_core_version'] }}. + > Please update your pipeline to the latest version. + > + > For more documentation on how to update your pipeline, please see the [nf-core documentation](https://github.com/nf-core/tools?tab=readme-ov-file#sync-a-pipeline-with-the-template) and [Synchronisation documentation](https://nf-co.re/docs/contributing/sync). # diff --git a/.gitignore b/.gitignore index 5124c9a..a42ce01 100644 --- a/.gitignore +++ b/.gitignore @@ -6,3 +6,4 @@ results/ testing/ testing* *.pyc +null/ diff --git a/.nf-core.yml b/.nf-core.yml index 36a5b95..7ab9250 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,6 @@ bump_version: null lint: null -nf_core_version: 3.0.1 +nf_core_version: 3.0.2 org_path: null repository_type: pipeline template: diff --git a/main.nf b/main.nf index 59f6c99..2f80241 100644 --- a/main.nf +++ b/main.nf @@ -76,7 +76,7 @@ workflow { params.outdir, params.input ) - + // // WORKFLOW: Run main workflow // diff --git a/modules.json b/modules.json index c58adec..accb0d3 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "b8d36829fa84b6e404364abff787e8b07f6d058c", + "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d", "installed_by": ["modules"] } } @@ -21,12 +21,12 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "9d05360da397692321d377b6102d2fb22507c6ef", + "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "772684d9d66f37b650c8ba5146ac1ee3ecba2acb", + "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba", "installed_by": ["subworkflows"] }, "utils_nfschema_plugin": { diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 9724d2f..cc0643e 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -52,7 +52,7 @@ process MULTIQC { stub: """ mkdir multiqc_data - touch multiqc_plots + mkdir multiqc_plots touch multiqc_report.html cat <<-END_VERSIONS > versions.yml diff --git a/nextflow.config b/nextflow.config index a8b79da..c89633f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -254,10 +254,10 @@ validation { """ afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { " https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""} * The nf-core framework - https://doi.org/10.1038/s41587-020-0439-x + https://doi.org/10.1038/s41587-020-0439-x * Software dependencies - https://github.com/${manifest.name}/blob/master/CITATIONS.md + https://github.com/${manifest.name}/blob/master/CITATIONS.md """ } summary { diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index dfe900c..fa469fa 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -47,7 +47,6 @@ workflow PIPELINE_INITIALISATION { workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 ) - // // Validate parameters and generate parameter summary to stdout // @@ -56,7 +55,6 @@ workflow PIPELINE_INITIALISATION { validate_params, null ) - // // Check config provided to the pipeline @@ -64,6 +62,7 @@ workflow PIPELINE_INITIALISATION { UTILS_NFCORE_PIPELINE ( nextflow_cli_args ) + // // Custom validation for pipeline parameters // @@ -110,7 +109,6 @@ workflow PIPELINE_COMPLETION { email // string: email address email_on_fail // string: email address sent on pipeline failure plaintext_email // boolean: Send plain-text email instead of HTML - outdir // path: Path to output directory where results will be published monochrome_logs // boolean: Disable ANSI colour codes in log output hook_url // string: hook URL for notifications diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index 2b0dc67..0fcbf7b 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -3,9 +3,9 @@ // /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NEXTFLOW_PIPELINE { @@ -44,9 +44,9 @@ workflow UTILS_NEXTFLOW_PIPELINE { } /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // @@ -106,17 +106,19 @@ def checkCondaChannels() { def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order - def channel_priority_violation = false - - required_channels_in_order.eachWithIndex { channel, index -> - if (index < required_channels_in_order.size() - 1) { - channel_priority_violation |= !(channels.indexOf(channel) < channels.indexOf(required_channels_in_order[index + 1])) - } - } + def channel_priority_violation = required_channels_in_order != channels.findAll { ch -> ch in required_channels_in_order } if (channels_missing | channel_priority_violation) { - log.warn( - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + " There is a problem with your Conda configuration!\n\n" + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + " Please refer to https://bioconda.github.io/\n" + " The observed channel order is \n" + " ${channels}\n" + " but the following channel order is required:\n" + " ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - ) + log.warn """\ + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + There is a problem with your Conda configuration! + You will need to set-up the conda-forge and bioconda channels correctly. + Please refer to https://bioconda.github.io/ + The observed channel order is + ${channels} + but the following channel order is required: + ${required_channels_in_order} + ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + """.stripIndent(true) } } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index b78273c..5cb7baf 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -3,9 +3,9 @@ // /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ SUBWORKFLOW DEFINITION -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ workflow UTILS_NFCORE_PIPELINE { @@ -21,9 +21,9 @@ workflow UTILS_NFCORE_PIPELINE { } /* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ // @@ -62,7 +62,7 @@ def checkProfileProvided(nextflow_cli_args) { def workflowCitation() { def temp_doi_ref = "" def manifest_doi = workflow.manifest.doi.tokenize(",") - // Using a loop to handle multiple DOIs + // Handling multiple DOIs // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers // Removing ` ` since the manifest.doi is a string and not a proper list manifest_doi.each { doi_ref -> diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 2f01cfa..0ba2091 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -57,13 +57,11 @@ workflow VARIANTBENCHMARKING { Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() - summary_params = paramsSummaryMap( workflow, parameters_schema: "nextflow_schema.json") ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) ch_multiqc_files = ch_multiqc_files.mix( ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) From 7f17e59e69969af2685ad7c62ce412b539c7175e Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 28 Oct 2024 14:50:18 +0100 Subject: [PATCH 184/292] initial copy from Famke --- assets/datavzrd/datavzrd.template.yaml | 28 +++ modules.json | 5 + modules/local/create_datavzrd_input/main.nf | 18 ++ modules/nf-core/datavzrd/environment.yml | 5 + modules/nf-core/datavzrd/main.nf | 63 +++++++ modules/nf-core/datavzrd/meta.yml | 44 +++++ modules/nf-core/datavzrd/tests/main.nf.test | 112 ++++++++++++ .../nf-core/datavzrd/tests/main.nf.test.snap | 162 ++++++++++++++++++ modules/nf-core/datavzrd/tests/tags.yml | 2 + .../local/report_benchmark_statistics.nf | 21 ++- 10 files changed, 458 insertions(+), 2 deletions(-) create mode 100644 assets/datavzrd/datavzrd.template.yaml create mode 100644 modules/local/create_datavzrd_input/main.nf create mode 100644 modules/nf-core/datavzrd/environment.yml create mode 100644 modules/nf-core/datavzrd/main.nf create mode 100644 modules/nf-core/datavzrd/meta.yml create mode 100644 modules/nf-core/datavzrd/tests/main.nf.test create mode 100644 modules/nf-core/datavzrd/tests/main.nf.test.snap create mode 100644 modules/nf-core/datavzrd/tests/tags.yml diff --git a/assets/datavzrd/datavzrd.template.yaml b/assets/datavzrd/datavzrd.template.yaml new file mode 100644 index 0000000..bfd6d79 --- /dev/null +++ b/assets/datavzrd/datavzrd.template.yaml @@ -0,0 +1,28 @@ +datasets: + report: + path: CSVPATH #svbenchmark.sv.summary.csv + separator: "," +views: + test: + dataset: report + render-table: + columns: + Tool: + display-mode: normal + Threshold: + display-mode: normal + TP_base: + display-mode: normal + FN: + display-mode: normal + TP_call: + display-mode: normal + FP: + display-mode: normal + Precision: + display-mode: normal + Recall: + display-mode: normal + F1: + display-mode: normal +# FP_gt,FP_al,Recall,Precision,Frac_NA,F1,TRUTH_TiTv_ratio,QUERY_TiTv_ratio,TRUTH_het_hom_ratio,QUERY_het_hom_ratio diff --git a/modules.json b/modules.json index 52b8658..c8ebf59 100644 --- a/modules.json +++ b/modules.json @@ -55,6 +55,11 @@ "git_sha": "a5377837fe9013bde89de8689829e83e84086536", "installed_by": ["modules"] }, + "datavzrd": { + "branch": "master", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "installed_by": ["modules"] + }, "happy/happy": { "branch": "master", "git_sha": "41fc46dfd94dddf4fdee633629090c1c3bc9f668", diff --git a/modules/local/create_datavzrd_input/main.nf b/modules/local/create_datavzrd_input/main.nf new file mode 100644 index 0000000..74fb4ec --- /dev/null +++ b/modules/local/create_datavzrd_input/main.nf @@ -0,0 +1,18 @@ +process CREATE_DATAVZRD_INPUT { + tag "$meta.id" + label 'process_single' + + input: + path(template) //tuple val(meta), + tuple val(meta), path(csv) + + output: + tuple val(meta), path("*.yaml"), path(csv), emit: config + + script: + """ + #!/bin/bash + + cat "$template" | sed "s|CSVPATH|$csv|g" > config.yaml + """ +} diff --git a/modules/nf-core/datavzrd/environment.yml b/modules/nf-core/datavzrd/environment.yml new file mode 100644 index 0000000..0659346 --- /dev/null +++ b/modules/nf-core/datavzrd/environment.yml @@ -0,0 +1,5 @@ +channels: + - conda-forge + - bioconda +dependencies: + - conda-forge::datavzrd==2.36.12 diff --git a/modules/nf-core/datavzrd/main.nf b/modules/nf-core/datavzrd/main.nf new file mode 100644 index 0000000..a8a6261 --- /dev/null +++ b/modules/nf-core/datavzrd/main.nf @@ -0,0 +1,63 @@ +process DATAVZRD { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'oras://community.wave.seqera.io/library/datavzrd:2.36.12--bb93c8c988b7a9af': + 'community.wave.seqera.io/library/datavzrd:2.36.12--593eb75e566b7f2a' }" + + input: + tuple val(meta), file(config_file), file(table) + + output: + tuple val(meta), path("${prefix}"), emit: report + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + prefix = task.ext.prefix ?: "${meta.id}" + """ + mkdir ${prefix} + datavzrd \\ + ${args} \\ + ${config_file} \\ + --output ${prefix} \\ + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + datavzrd: \$(echo \$( datavzrd --version | sed -e 's/[^0-9.]//g' )) + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + """ + mkdir ${prefix} + mkdir ${prefix}/static + mkdir ${prefix}/network + mkdir ${prefix}/network/data + mkdir ${prefix}/network/plots + touch ./${prefix}/index.html + touch ./${prefix}/static/bootstrap.min.css + touch ./${prefix}/static/bootstrap-select.min.css + touch ./${prefix}/static/bootstrap-table.min.css + touch ./${prefix}/static/bootstrap-table-fixed-columns.min.css + touch ./${prefix}/static/bundle.js + touch ./${prefix}/static/datavzrd.css + touch ./${prefix}/network/index_1.html + touch ./${prefix}/network/config.js + touch ./${prefix}/network/functions.js + touch ./${prefix}/network/heatmap.js + touch ./${prefix}/network/data/data_1.js + touch ./${prefix}/network/plots/plot_0.js + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + datavzrd: \$(echo \$( datavzrd --version | sed -e 's/[^0-9.]//g' )) + END_VERSIONS + """ +} diff --git a/modules/nf-core/datavzrd/meta.yml b/modules/nf-core/datavzrd/meta.yml new file mode 100644 index 0000000..44d6bd6 --- /dev/null +++ b/modules/nf-core/datavzrd/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "datavzrd" +description: Datavzrd is a tool to create visual HTML reports from collections of + CSV/TSV tables. +keywords: + - visualisation + - tsv + - csv +tools: + - datavzrd: + description: Datavzrd is a tool to create visual HTML reports from collections + of CSV/TSV tables. + homepage: https://datavzrd.github.io/ + documentation: https://datavzrd.github.io/docs/index.html + tool_dev_url: https://github.com/datavzrd/datavzrd + licence: ["MIT"] + identifier: "" + +input: + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] +output: + - report: + - meta: + type: directory + description: directory with HTML report of provided CSV/TSV files + pattern: "/output/" + - ${prefix}: + type: directory + description: directory with HTML report of provided CSV/TSV files + pattern: "/output/" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@vickylaram" + - "@famosab" +maintainers: + - "@famosab" diff --git a/modules/nf-core/datavzrd/tests/main.nf.test b/modules/nf-core/datavzrd/tests/main.nf.test new file mode 100644 index 0000000..86bd849 --- /dev/null +++ b/modules/nf-core/datavzrd/tests/main.nf.test @@ -0,0 +1,112 @@ +nextflow_process { + name "Test Process DATAVZRD" + script "../main.nf" + process "DATAVZRD" + tag "modules" + tag "modules_nfcore" + tag "datavzrd" + + test("csv") { + + when { + process { + """ + input[0] = [ [id: "csv_config"], + file(params.modules_testdata_base_path + 'generic/config/config_test.datavzrd.yaml', checkIfExists: true), + file(params.modules_testdata_base_path + 'generic/csv/test.csv', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + path(process.out.report[0][1]) + .list() + .collect { getRecursiveFileNames(it, process.out.report[0][1]) } + .flatten() + ).match("csv") + } + ) + } + } + + test("tsv") { + + when { + process { + """ + input[0] = [ [id: "tsv_config"], + file(params.modules_testdata_base_path + 'generic/config/config_network.datavzrd.yaml', checkIfExists: true), + file(params.modules_testdata_base_path + 'generic/tsv/network.tsv', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + path(process.out.report[0][1]) + .list() + .collect { getRecursiveFileNames(it, process.out.report[0][1]) } + .flatten() + ).match("tsv") + } + ) + } + } + + test("csv - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ [id: "csv_config"], + file(params.modules_testdata_base_path + 'generic/config/config_test.datavzrd.yaml', checkIfExists: true), + file(params.modules_testdata_base_path + 'generic/csv/test.csv', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("no_config_file") { + when { + process { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert process.failed }, + { assert process.out['report'].isEmpty() }, + { assert snapshot(process.out).match() } + ) + } + } +} + +def getRecursiveFileNames(fileOrDir, outputDir) { + if(file(fileOrDir.toString()).isDirectory()) { + return fileOrDir.list().collect { getRecursiveFileNames(it, outputDir) } + } + return fileOrDir.toString().replace("${outputDir}/", "") +} diff --git a/modules/nf-core/datavzrd/tests/main.nf.test.snap b/modules/nf-core/datavzrd/tests/main.nf.test.snap new file mode 100644 index 0000000..d57df8a --- /dev/null +++ b/modules/nf-core/datavzrd/tests/main.nf.test.snap @@ -0,0 +1,162 @@ +{ + "csv - stub": { + "content": [ + { + "0": [ + [ + { + "id": "csv_config" + }, + [ + "index.html:md5,d41d8cd98f00b204e9800998ecf8427e", + [ + "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", + [ + "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", + "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", + "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", + [ + "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + [ + "bootstrap-select.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bootstrap-table-fixed-columns.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bootstrap-table.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bootstrap.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bundle.js:md5,d41d8cd98f00b204e9800998ecf8427e", + "datavzrd.css:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ] + ], + "1": [ + "versions.yml:md5,4c539593d78c0a07ede5759e0ce5ade8" + ], + "report": [ + [ + { + "id": "csv_config" + }, + [ + "index.html:md5,d41d8cd98f00b204e9800998ecf8427e", + [ + "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", + [ + "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", + "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", + "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", + [ + "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + [ + "bootstrap-select.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bootstrap-table-fixed-columns.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bootstrap-table.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bootstrap.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", + "bundle.js:md5,d41d8cd98f00b204e9800998ecf8427e", + "datavzrd.css:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ] + ], + "versions": [ + "versions.yml:md5,4c539593d78c0a07ede5759e0ce5ade8" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-06-26T10:16:55.267548327" + }, + "tsv": { + "content": [ + [ + "versions.yml:md5,4c539593d78c0a07ede5759e0ce5ade8" + ], + [ + "index.html", + "network/config.js", + "network/data/data_1.js", + "network/functions.js", + "network/heatmap.js", + "network/index_1.html", + "network/plots/plot_0.js", + "network/plots/plot_1.js", + "network/plots/plot_2.js", + "network/plots/plot_3.js", + "static/bootstrap-select.min.css", + "static/bootstrap-table-fixed-columns.min.css", + "static/bootstrap-table.min.css", + "static/bootstrap.min.css", + "static/bundle.js", + "static/datavzrd.css" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-06-26T10:15:02.495557702" + }, + "no_config_file": { + "content": [ + { + "0": [ + + ], + "1": [ + + ], + "report": [ + + ], + "versions": [ + + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-06-26T10:15:09.362847938" + }, + "csv": { + "content": [ + [ + "versions.yml:md5,4c539593d78c0a07ede5759e0ce5ade8" + ], + [ + "index.html", + "static/bootstrap-select.min.css", + "static/bootstrap-table-fixed-columns.min.css", + "static/bootstrap-table.min.css", + "static/bootstrap.min.css", + "static/bundle.js", + "static/datavzrd.css", + "test/config.js", + "test/data/data_1.js", + "test/functions.js", + "test/heatmap.js", + "test/index_1.html", + "test/plots/plot_0.js", + "test/plots/plot_1.js", + "test/plots/plot_2.js", + "test/plots/plot_3.js" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-06-26T10:14:58.495338891" + } +} \ No newline at end of file diff --git a/modules/nf-core/datavzrd/tests/tags.yml b/modules/nf-core/datavzrd/tests/tags.yml new file mode 100644 index 0000000..e6eeb99 --- /dev/null +++ b/modules/nf-core/datavzrd/tests/tags.yml @@ -0,0 +1,2 @@ +datavzrd: + - "modules/nf-core/datavzrd/**" diff --git a/subworkflows/local/report_benchmark_statistics.nf b/subworkflows/local/report_benchmark_statistics.nf index c0efa10..33a9a82 100644 --- a/subworkflows/local/report_benchmark_statistics.nf +++ b/subworkflows/local/report_benchmark_statistics.nf @@ -2,8 +2,10 @@ // REPORT_BENCHMARK_STATISTICS: SUMMARIZE BENCHMARK REPORTS // -include { MERGE_REPORTS } from '../../modules/local/merge_reports' -include { PLOTS } from '../../modules/local/plots' +include { MERGE_REPORTS } from '../../modules/local/merge_reports' +include { PLOTS } from '../../modules/local/plots' +include { CREATE_DATAVZRD_INPUT } from '../../modules/local/create_datavzrd_input' addParams( options: params.options ) +include { DATAVZRD } from '../../modules/nf-core/datavzrd' addParams( options: params.options ) workflow REPORT_BENCHMARK_STATISTICS { take: @@ -24,6 +26,21 @@ workflow REPORT_BENCHMARK_STATISTICS { ) versions = versions.mix(PLOTS.out.versions.first()) + // add path to csv file to the datavzrd input + template = Channel.fromPath( "$projectDir/assets/datavzrd/datavzrd.template.yaml", checkIfExists:true) + CREATE_DATAVZRD_INPUT ( + template, + MERGE_REPORTS.out.summary + ) + + // use datavzrd to render the report based on the create input + // input consists of config file and the table itself + DATAVZRD ( + CREATE_DATAVZRD_INPUT.out.config + ) + versions = versions.mix(DATAVZRD.out.versions.first()) + + datavzrd_report = DATAVZRD.out.report emit: versions From 307faef8eeed8892219d9245c6dedc19a5e64c4c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 29 Oct 2024 10:50:01 +0100 Subject: [PATCH 185/292] correct data types in merged report --- bin/merge_reports.py | 62 ++++++++++++++++++++++++++------------------ 1 file changed, 37 insertions(+), 25 deletions(-) diff --git a/bin/merge_reports.py b/bin/merge_reports.py index 987e91c..e28708a 100755 --- a/bin/merge_reports.py +++ b/bin/merge_reports.py @@ -56,10 +56,10 @@ def get_svbenchmark_resuls(file_paths): # Initialize a dictionary to store the data data = { 'Tool': [filename.split(".")[0]], - 'TP_base': [DTP_match.group(1) if DTP_match else 'NA'], - 'FP': [FP_match.group(1) if FP_match else 'NA'], - 'TP_comp': [DTP_match.group(1) if DTP_match else 'NA'], - 'FN': [FN_match.group(1) if FN_match else 'NA'], + 'TP_base': [int(DTP_match.group(1)) if DTP_match else 'NA'], + 'FP': [int(FP_match.group(1)) if FP_match else 'NA'], + 'TP_comp': [int(DTP_match.group(1)) if DTP_match else 'NA'], + 'FN': [int(FN_match.group(1)) if FN_match else 'NA'], 'Recall': [float(recall_match.group(1))/100 if recall_match else 'NA'], 'Precision': [float(precision_match.group(1))/100 if precision_match else 'NA'], 'F1': [float(f1_match.group(1)) if f1_match else 'NA']} @@ -85,13 +85,13 @@ def get_truvari_resuls(file_paths): relevant_data = { "Tool": filename.split(".")[0], - "TP_base": data["TP-base"].iloc[0], - "TP_comp": data["TP-comp"].iloc[0], - "FP": data["FP"].iloc[0], - "FN": data["FN"].iloc[0], - "Precision": data["precision"].iloc[0], - "Recall": data["recall"].iloc[0], - "F1": data["f1"].iloc[0]} + "TP_base": int(data["TP-base"].iloc[0]), + "TP_comp": int(data["TP-comp"].iloc[0]), + "FP": int(data["FP"].iloc[0]), + "FN": int(data["FN"].iloc[0]), + "Precision": float(data["precision"].iloc[0]), + "Recall": float(data["recall"].iloc[0]), + "F1": float(data["f1"].iloc[0])} df = pd.DataFrame([relevant_data]) merged_df = pd.concat([merged_df, df]) @@ -102,7 +102,18 @@ def get_wittyer_resuls(file_paths): # Initialize an empty DataFrame to store the merged data merged_df = pd.DataFrame() - # Iterate over each table file + header = lines[0].strip().split() + + # Extract data + data = [] + for line in lines[2:]: + data.append(line.strip().split()) + + # Create DataFrame + df = pd.DataFrame(data, columns=header) + df['Tool'] = filename.split(".")[0] + df_redesigned = df[['Tool', 'Threshold','True-pos-baseline','True-pos-call','False-pos','False-neg','Precision','Sensitivity','F-measure']] + df_redesigned.columns = ['Tool', 'Threshold','TP_base','TP_call','FP','FN','Precision','Recall','F1']# Iterate over each table file for file in file_paths: # Read the json into a DataFrame filename = os.path.basename(file) @@ -141,18 +152,7 @@ def get_rtgtools_resuls(file_paths): lines = f.readlines() # Extract header - header = lines[0].strip().split() - # Extract data - data = [] - for line in lines[2:]: - data.append(line.strip().split()) - - # Create DataFrame - df = pd.DataFrame(data, columns=header) - df['Tool'] = filename.split(".")[0] - df_redesigned = df[['Tool', 'Threshold','True-pos-baseline','True-pos-call','False-pos','False-neg','Precision','Sensitivity','F-measure']] - df_redesigned.columns = ['Tool', 'Threshold','TP_base','TP_call','FP','FN','Precision','Recall','F1'] merged_df = pd.concat([merged_df, df_redesigned]) return merged_df @@ -172,7 +172,14 @@ def get_happy_resuls(file_paths): df_redesigned = df[['Tool', 'Type','Filter','TRUTH.TOTAL','TRUTH.TP','TRUTH.FN','QUERY.TOTAL','QUERY.FP','QUERY.UNK','FP.gt','FP.al','METRIC.Recall','METRIC.Precision','METRIC.Frac_NA','METRIC.F1_Score','TRUTH.TOTAL.TiTv_ratio','QUERY.TOTAL.TiTv_ratio','TRUTH.TOTAL.het_hom_ratio','QUERY.TOTAL.het_hom_ratio']] df_redesigned.columns = ['Tool', 'Type','Filter','TP_base','TP','FN','TP_call','FP','UNK','FP_gt','FP_al','Recall','Precision','Frac_NA','F1','TRUTH_TiTv_ratio','QUERY_TiTv_ratio','TRUTH_het_hom_ratio','QUERY_het_hom_ratio'] - merged_df = pd.concat([merged_df, df_redesigned]) + # Convert relevant columns to integers, handling potential NaN values + int_columns = ['TP_base', 'TP', 'FN', 'TP_call', 'FP', 'UNK', 'FP_gt', 'FP_al'] + float_columns = ['Recall','Precision','Frac_NA','F1','TRUTH_TiTv_ratio','QUERY_TiTv_ratio','TRUTH_het_hom_ratio','QUERY_het_hom_ratio'] + df_redesigned[int_columns] = df_redesigned[int_columns].fillna(0).astype(int) + df_redesigned[float_columns] = df_redesigned[float_columns].fillna(0).astype(float) + + # Concatenate with the merged DataFrame + merged_df = pd.concat([merged_df, df_redesigned], ignore_index=True) return merged_df @@ -189,8 +196,13 @@ def get_sompy_resuls(file_paths, vartype): df['Tool'] = filename.split(".")[0] df_redesigned = df[['Tool','type','total.truth','tp','fn','total.query','fp','unk','recall','precision','recall_lower','recall_upper','recall2','precision_lower','precision_upper','na','ambiguous','fp.region.size','fp.rate']] df_redesigned.columns = ['Tool','Type','TP_base','TP','FN','TP_call','FP','UNK','Recall','Precision','recall_lower','recall_upper','recall2','precision_lower','precision_upper','na','ambiguous','fp.region.size','fp.rate'] + # Convert relevant columns to integers, handling potential NaN values + int_columns = ['TP_base', 'TP', 'FN', 'TP_call', 'FP', 'UNK'] + float_columns = ['Recall','Precision','recall_lower','recall_upper','recall2','precision_lower','precision_upper','na','ambiguous','fp.region.size','fp.rate'] + df_redesigned[int_columns] = df_redesigned[int_columns].fillna(0).astype(int) + df_redesigned[float_columns] = df_redesigned[float_columns].fillna(0).astype(float) - merged_df = pd.concat([merged_df, df_redesigned]) + merged_df = pd.concat([merged_df, df_redesigned], ignore_index=True) if vartype == "snv": merged_df1 = merged_df[merged_df["Type"] == 'SNVs'] From e06acc93bc49465da7f688d77a449a65b3bda240 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 29 Oct 2024 11:10:13 +0000 Subject: [PATCH 186/292] add datavzrd yaml --- assets/datavzrd/happy.datavzrd.template.yaml | 46 ++++++++++++++++ .../datavzrd/rtgtools.datavzrd.template.yaml | 28 ++++++++++ ...late.yaml => sompy.datavzrd.template.yaml} | 0 .../svbenchmark.datavzrd.template.yaml | 26 +++++++++ .../datavzrd/truvari.datavzrd.template.yaml | 26 +++++++++ .../datavzrd/wittyer.datavzrd.template.yaml | 20 +++++++ bin/merge_reports.py | 53 ++++++++++--------- conf/modules.config | 8 +++ modules/local/create_datavzrd_input/main.nf | 3 +- .../local/report_benchmark_statistics.nf | 14 +++-- workflows/variantbenchmarking.nf | 2 +- 11 files changed, 196 insertions(+), 30 deletions(-) create mode 100644 assets/datavzrd/happy.datavzrd.template.yaml create mode 100644 assets/datavzrd/rtgtools.datavzrd.template.yaml rename assets/datavzrd/{datavzrd.template.yaml => sompy.datavzrd.template.yaml} (100%) create mode 100644 assets/datavzrd/svbenchmark.datavzrd.template.yaml create mode 100644 assets/datavzrd/truvari.datavzrd.template.yaml create mode 100644 assets/datavzrd/wittyer.datavzrd.template.yaml diff --git a/assets/datavzrd/happy.datavzrd.template.yaml b/assets/datavzrd/happy.datavzrd.template.yaml new file mode 100644 index 0000000..143776b --- /dev/null +++ b/assets/datavzrd/happy.datavzrd.template.yaml @@ -0,0 +1,46 @@ +datasets: + report: + path: CSVPATH #svbenchmark.sv.summary.csv + separator: "," +views: + test: + dataset: report + render-table: + columns: + Tool: + display-mode: normal + Type: + display-mode: normal + Filter: + display-mode: normal + TP_base: + display-mode: normal + FN: + display-mode: normal + TP_call: + display-mode: normal + FP: + display-mode: normal + UNK: + display-mode: normal + Precision: + display-mode: normal + Recall: + display-mode: normal + F1: + display-mode: normal + FP_gt: + display-mode: normal + FP_al: + display-mode: normal + Frac_NA: + display-mode: normal + TRUTH_TiTv_ratio: + display-mode: normal + QUERY_TiTv_ratio: + display-mode: normal + TRUTH_het_hom_ratio: + display-mode: normal + QUERY_het_hom_ratio: + display-mode: normal +# Tool,Type,Filter,TP_base,TP,FN,TP_call,FP,UNK,FP_gt,FP_al,Recall,Precision,Frac_NA,F1,TRUTH_TiTv_ratio,QUERY_TiTv_ratio,TRUTH_het_hom_ratio,QUERY_het_hom_ratio diff --git a/assets/datavzrd/rtgtools.datavzrd.template.yaml b/assets/datavzrd/rtgtools.datavzrd.template.yaml new file mode 100644 index 0000000..052abe3 --- /dev/null +++ b/assets/datavzrd/rtgtools.datavzrd.template.yaml @@ -0,0 +1,28 @@ +datasets: + report: + path: CSVPATH #svbenchmark.sv.summary.csv + separator: "," +views: + test: + dataset: report + render-table: + columns: + Tool: + display-mode: normal + Threshold: + display-mode: normal + TP_base: + display-mode: normal + FN: + display-mode: normal + TP_call: + display-mode: normal + FP: + display-mode: normal + Precision: + display-mode: normal + Recall: + display-mode: normal + F1: + display-mode: normal +# Tool,Threshold,TP_base,TP_call,FP,FN,Precision,Recall,F1 diff --git a/assets/datavzrd/datavzrd.template.yaml b/assets/datavzrd/sompy.datavzrd.template.yaml similarity index 100% rename from assets/datavzrd/datavzrd.template.yaml rename to assets/datavzrd/sompy.datavzrd.template.yaml diff --git a/assets/datavzrd/svbenchmark.datavzrd.template.yaml b/assets/datavzrd/svbenchmark.datavzrd.template.yaml new file mode 100644 index 0000000..fcba85b --- /dev/null +++ b/assets/datavzrd/svbenchmark.datavzrd.template.yaml @@ -0,0 +1,26 @@ +datasets: + report: + path: CSVPATH #svbenchmark.sv.summary.csv + separator: "," +views: + test: + dataset: report + render-table: + columns: + Tool: + display-mode: normal + TP_base: + display-mode: normal + FN: + display-mode: normal + TP_comp: + display-mode: normal + FP: + display-mode: normal + Precision: + display-mode: normal + Recall: + display-mode: normal + F1: + display-mode: normal +# Tool,TP_base,FP,TP_comp,FN,Recall,Precision,F1 diff --git a/assets/datavzrd/truvari.datavzrd.template.yaml b/assets/datavzrd/truvari.datavzrd.template.yaml new file mode 100644 index 0000000..0875dc3 --- /dev/null +++ b/assets/datavzrd/truvari.datavzrd.template.yaml @@ -0,0 +1,26 @@ +datasets: + report: + path: CSVPATH #svbenchmark.sv.summary.csv + separator: "," +views: + test: + dataset: report + render-table: + columns: + Tool: + display-mode: normal + TP_base: + display-mode: normal + FN: + display-mode: normal + TP_comp: + display-mode: normal + FP: + display-mode: normal + Precision: + display-mode: normal + Recall: + display-mode: normal + F1: + display-mode: normal +# Tool,TP_base,TP_call,FP,FN,Precision,Recall,F1 diff --git a/assets/datavzrd/wittyer.datavzrd.template.yaml b/assets/datavzrd/wittyer.datavzrd.template.yaml new file mode 100644 index 0000000..0a528f1 --- /dev/null +++ b/assets/datavzrd/wittyer.datavzrd.template.yaml @@ -0,0 +1,20 @@ +datasets: + report: + path: CSVPATH #svbenchmark.sv.summary.csv + separator: "," +views: + test: + dataset: report + render-table: + columns: + Tool: + display-mode: normal + TP_base: + display-mode: normal + FN: + display-mode: normal + TP_comp: + display-mode: normal + FP: + display-mode: normal +# Tool,StatsType,TP_base,TP_comp,FP,FN,Precision,Recall,F1 diff --git a/bin/merge_reports.py b/bin/merge_reports.py index e28708a..0fd806b 100755 --- a/bin/merge_reports.py +++ b/bin/merge_reports.py @@ -66,7 +66,7 @@ def get_svbenchmark_resuls(file_paths): df = pd.DataFrame(data) - merged_df = pd.concat([merged_df, df]) + merged_df = pd.concat([merged_df, df], ignore_index=True) return merged_df @@ -94,7 +94,7 @@ def get_truvari_resuls(file_paths): "F1": float(data["f1"].iloc[0])} df = pd.DataFrame([relevant_data]) - merged_df = pd.concat([merged_df, df]) + merged_df = pd.concat([merged_df, df], ignore_index=True) return merged_df @@ -102,18 +102,6 @@ def get_wittyer_resuls(file_paths): # Initialize an empty DataFrame to store the merged data merged_df = pd.DataFrame() - header = lines[0].strip().split() - - # Extract data - data = [] - for line in lines[2:]: - data.append(line.strip().split()) - - # Create DataFrame - df = pd.DataFrame(data, columns=header) - df['Tool'] = filename.split(".")[0] - df_redesigned = df[['Tool', 'Threshold','True-pos-baseline','True-pos-call','False-pos','False-neg','Precision','Sensitivity','F-measure']] - df_redesigned.columns = ['Tool', 'Threshold','TP_base','TP_call','FP','FN','Precision','Recall','F1']# Iterate over each table file for file in file_paths: # Read the json into a DataFrame filename = os.path.basename(file) @@ -126,17 +114,17 @@ def get_wittyer_resuls(file_paths): relevant_data.append({ "Tool": filename.split(".")[0], "StatsType": stats["StatsType"], - "TP_base": stats["TruthTpCount"], - "TP_comp": stats["QueryTpCount"], - "FP": stats["QueryFpCount"], - "FN": stats["TruthFnCount"], - "Precision": stats["Precision"], - "Recall": stats["Recall"], - "F1": stats["Fscore"]} - ) + "TP_base": int(stats["TruthTpCount"]) if pd.notna(stats["TruthTpCount"]) else 0, + "TP_comp": int(stats["QueryTpCount"]) if pd.notna(stats["QueryTpCount"]) else 0, + "FP": int(stats["QueryFpCount"]) if pd.notna(stats["QueryFpCount"]) else 0, + "FN": int(stats["TruthFnCount"]) if pd.notna(stats["TruthFnCount"]) else 0, + "Precision": float(stats["Precision"]) if pd.notna(stats["Precision"]) else float('nan'), + "Recall": float(stats["Recall"]) if pd.notna(stats["Recall"]) else float('nan'), + "F1": float(stats["Fscore"]) if pd.notna(stats["Fscore"]) else float('nan') + }) df = pd.DataFrame(relevant_data) - merged_df = pd.concat([merged_df, df]) + merged_df = pd.concat([merged_df, df], ignore_index=True) return merged_df @@ -152,9 +140,26 @@ def get_rtgtools_resuls(file_paths): lines = f.readlines() # Extract header + header = lines[0].strip().split() + + # Extract data + data = [] + for line in lines[2:]: + data.append(line.strip().split()) + # Create DataFrame + df = pd.DataFrame(data, columns=header) + df['Tool'] = filename.split(".")[0] + df_redesigned = df[['Tool', 'Threshold','True-pos-baseline','True-pos-call','False-pos','False-neg','Precision','Sensitivity','F-measure']] + df_redesigned.columns = ['Tool', 'Threshold','TP_base','TP_call','FP','FN','Precision','Recall','F1'] + # Convert relevant columns to integers, handling potential NaN values + int_columns = ['TP_base', 'FN', 'TP_call', 'FP'] + float_columns = ['Recall','Precision','F1'] + df_redesigned[int_columns] = df_redesigned[int_columns].fillna(0).astype(int) + df_redesigned[float_columns] = df_redesigned[float_columns].fillna(0).astype(float) + + merged_df = pd.concat([merged_df, df_redesigned], ignore_index=True) - merged_df = pd.concat([merged_df, df_redesigned]) return merged_df def get_happy_resuls(file_paths): diff --git a/conf/modules.config b/conf/modules.config index ae2799b..e7cf06d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -277,6 +277,14 @@ process { mode: params.publish_dir_mode ] } + withName: DATAVZRD { + ext.prefix = {"${meta.id}"} + publishDir = [ + path: {"${params.outdir}/summary/datavzrd/${meta.vartype}/${meta.id}"}, + pattern: "*", + mode: params.publish_dir_mode + ] + } // compare vcf results withName: "TABIX_BGZIP*"{ ext.prefix = {input.toString() - ".vcf.gz"} diff --git a/modules/local/create_datavzrd_input/main.nf b/modules/local/create_datavzrd_input/main.nf index 74fb4ec..d19edea 100644 --- a/modules/local/create_datavzrd_input/main.nf +++ b/modules/local/create_datavzrd_input/main.nf @@ -3,8 +3,7 @@ process CREATE_DATAVZRD_INPUT { label 'process_single' input: - path(template) //tuple val(meta), - tuple val(meta), path(csv) + tuple val(meta), path(csv), path(template) output: tuple val(meta), path("*.yaml"), path(csv), emit: config diff --git a/subworkflows/local/report_benchmark_statistics.nf b/subworkflows/local/report_benchmark_statistics.nf index 33a9a82..8ed96dd 100644 --- a/subworkflows/local/report_benchmark_statistics.nf +++ b/subworkflows/local/report_benchmark_statistics.nf @@ -26,11 +26,19 @@ workflow REPORT_BENCHMARK_STATISTICS { ) versions = versions.mix(PLOTS.out.versions.first()) + MERGE_REPORTS.out.summary + .map { meta, file -> tuple([vartype: meta.vartype] + [id: meta.benchmark_tool], file) } + .set { summary } + // add path to csv file to the datavzrd input - template = Channel.fromPath( "$projectDir/assets/datavzrd/datavzrd.template.yaml", checkIfExists:true) + summary + .map { meta, summary -> + [ meta, summary, file("${projectDir}/assets/datavzrd/${meta.id}.datavzrd.template.yaml", checkIfExists:true) ] + } + .set {template} + CREATE_DATAVZRD_INPUT ( - template, - MERGE_REPORTS.out.summary + template ) // use datavzrd to render the report based on the create input diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index e38b64f..eca9072 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -361,7 +361,7 @@ workflow VARIANTBENCHMARKING { // Benchmarking spesific to small germline samples SMALL_GERMLINE_BENCHMARK( - bench_input.small, + bench_input.small.mix(bench_input.snv,bench_input.indel), fasta, fai, sdf From 1c9db22ab23f6e20e4abc0cb25dd7c94fe5cc2f7 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 29 Oct 2024 14:45:25 +0100 Subject: [PATCH 187/292] update confige with datavzrd --- assets/datavzrd/happy.datavzrd.template.yaml | 2 +- assets/datavzrd/rtgtools.datavzrd.template.yaml | 2 +- assets/datavzrd/sompy.datavzrd.template.yaml | 2 +- assets/datavzrd/truvari.datavzrd.template.yaml | 2 +- assets/datavzrd/wittyer.datavzrd.template.yaml | 2 +- conf/modules.config | 4 ++-- 6 files changed, 7 insertions(+), 7 deletions(-) diff --git a/assets/datavzrd/happy.datavzrd.template.yaml b/assets/datavzrd/happy.datavzrd.template.yaml index 143776b..e82aa53 100644 --- a/assets/datavzrd/happy.datavzrd.template.yaml +++ b/assets/datavzrd/happy.datavzrd.template.yaml @@ -1,6 +1,6 @@ datasets: report: - path: CSVPATH #svbenchmark.sv.summary.csv + path: CSVPATH #happy.sv.summary.csv separator: "," views: test: diff --git a/assets/datavzrd/rtgtools.datavzrd.template.yaml b/assets/datavzrd/rtgtools.datavzrd.template.yaml index 052abe3..638cba0 100644 --- a/assets/datavzrd/rtgtools.datavzrd.template.yaml +++ b/assets/datavzrd/rtgtools.datavzrd.template.yaml @@ -1,6 +1,6 @@ datasets: report: - path: CSVPATH #svbenchmark.sv.summary.csv + path: CSVPATH #rtgtools.sv.summary.csv separator: "," views: test: diff --git a/assets/datavzrd/sompy.datavzrd.template.yaml b/assets/datavzrd/sompy.datavzrd.template.yaml index bfd6d79..57ef189 100644 --- a/assets/datavzrd/sompy.datavzrd.template.yaml +++ b/assets/datavzrd/sompy.datavzrd.template.yaml @@ -1,6 +1,6 @@ datasets: report: - path: CSVPATH #svbenchmark.sv.summary.csv + path: CSVPATH #sompy.sv.summary.csv separator: "," views: test: diff --git a/assets/datavzrd/truvari.datavzrd.template.yaml b/assets/datavzrd/truvari.datavzrd.template.yaml index 0875dc3..fc85922 100644 --- a/assets/datavzrd/truvari.datavzrd.template.yaml +++ b/assets/datavzrd/truvari.datavzrd.template.yaml @@ -1,6 +1,6 @@ datasets: report: - path: CSVPATH #svbenchmark.sv.summary.csv + path: CSVPATH #truvari.sv.summary.csv separator: "," views: test: diff --git a/assets/datavzrd/wittyer.datavzrd.template.yaml b/assets/datavzrd/wittyer.datavzrd.template.yaml index 0a528f1..eb68464 100644 --- a/assets/datavzrd/wittyer.datavzrd.template.yaml +++ b/assets/datavzrd/wittyer.datavzrd.template.yaml @@ -1,6 +1,6 @@ datasets: report: - path: CSVPATH #svbenchmark.sv.summary.csv + path: CSVPATH #wittyer.sv.summary.csv separator: "," views: test: diff --git a/conf/modules.config b/conf/modules.config index e7cf06d..cbd9898 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -280,7 +280,7 @@ process { withName: DATAVZRD { ext.prefix = {"${meta.id}"} publishDir = [ - path: {"${params.outdir}/summary/datavzrd/${meta.vartype}/${meta.id}"}, + path: {"${params.outdir}/summary/datavzrd/${meta.vartype}"}, pattern: "*", mode: params.publish_dir_mode ] @@ -355,7 +355,7 @@ process { // Don't publish results for these processes // process { - withName: 'TABIX_TABIX|TABIX_BGZIP|TABIX_BGZIPTABIX|BGZIP_TABIX|SURVIVOR_MERGE|BCFTOOLS_MERGE|REFORMAT_HEADER|BCFTOOLS_NORM|BCFTOOLS_DEDUP|BCFTOOLS_REHEADER|SORT_BED|UCSC_LIFTOVER|PICARD_LIFTOVERVCF|BCFTOOLS_VIEW_SUBSAMPLE' { + withName: 'TABIX_TABIX|TABIX_BGZIP|TABIX_BGZIPTABIX|BGZIP_TABIX|SURVIVOR_MERGE|BCFTOOLS_MERGE|REFORMAT_HEADER|BCFTOOLS_NORM|BCFTOOLS_DEDUP|BCFTOOLS_REHEADER|SORT_BED|UCSC_LIFTOVER|PICARD_LIFTOVERVCF|BCFTOOLS_VIEW_SUBSAMPLE|CREATE_DATAVZRD_INPUT' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false From 998e004ea960b46480e6dd5983a4cca95bd3e03a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 29 Oct 2024 14:54:33 +0100 Subject: [PATCH 188/292] fix template version --- .github/workflows/template_version_comment.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml index 9dea41f..d01fb43 100644 --- a/.github/workflows/template_version_comment.yml +++ b/.github/workflows/template_version_comment.yml @@ -12,7 +12,7 @@ jobs: uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Read template version from .nf-core.yml - uses: pietrobolcato/action-read-yaml@1.0.0 + uses: pietrobolcato/action-read-yaml@1.1.0 id: read_yml with: config: ${{ github.workspace }}/.nf-core.yml From fad3640242b17f45b99c887e2556f22b41c169ab Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 29 Oct 2024 15:09:22 +0100 Subject: [PATCH 189/292] deleet comments --- assets/datavzrd/happy.datavzrd.template.yaml | 1 - assets/datavzrd/rtgtools.datavzrd.template.yaml | 1 - assets/datavzrd/sompy.datavzrd.template.yaml | 1 - assets/datavzrd/svbenchmark.datavzrd.template.yaml | 1 - assets/datavzrd/truvari.datavzrd.template.yaml | 1 - assets/datavzrd/wittyer.datavzrd.template.yaml | 1 - 6 files changed, 6 deletions(-) diff --git a/assets/datavzrd/happy.datavzrd.template.yaml b/assets/datavzrd/happy.datavzrd.template.yaml index e82aa53..543c667 100644 --- a/assets/datavzrd/happy.datavzrd.template.yaml +++ b/assets/datavzrd/happy.datavzrd.template.yaml @@ -43,4 +43,3 @@ views: display-mode: normal QUERY_het_hom_ratio: display-mode: normal -# Tool,Type,Filter,TP_base,TP,FN,TP_call,FP,UNK,FP_gt,FP_al,Recall,Precision,Frac_NA,F1,TRUTH_TiTv_ratio,QUERY_TiTv_ratio,TRUTH_het_hom_ratio,QUERY_het_hom_ratio diff --git a/assets/datavzrd/rtgtools.datavzrd.template.yaml b/assets/datavzrd/rtgtools.datavzrd.template.yaml index 638cba0..f55ec84 100644 --- a/assets/datavzrd/rtgtools.datavzrd.template.yaml +++ b/assets/datavzrd/rtgtools.datavzrd.template.yaml @@ -25,4 +25,3 @@ views: display-mode: normal F1: display-mode: normal -# Tool,Threshold,TP_base,TP_call,FP,FN,Precision,Recall,F1 diff --git a/assets/datavzrd/sompy.datavzrd.template.yaml b/assets/datavzrd/sompy.datavzrd.template.yaml index 57ef189..ac3eaf6 100644 --- a/assets/datavzrd/sompy.datavzrd.template.yaml +++ b/assets/datavzrd/sompy.datavzrd.template.yaml @@ -25,4 +25,3 @@ views: display-mode: normal F1: display-mode: normal -# FP_gt,FP_al,Recall,Precision,Frac_NA,F1,TRUTH_TiTv_ratio,QUERY_TiTv_ratio,TRUTH_het_hom_ratio,QUERY_het_hom_ratio diff --git a/assets/datavzrd/svbenchmark.datavzrd.template.yaml b/assets/datavzrd/svbenchmark.datavzrd.template.yaml index fcba85b..f935e62 100644 --- a/assets/datavzrd/svbenchmark.datavzrd.template.yaml +++ b/assets/datavzrd/svbenchmark.datavzrd.template.yaml @@ -23,4 +23,3 @@ views: display-mode: normal F1: display-mode: normal -# Tool,TP_base,FP,TP_comp,FN,Recall,Precision,F1 diff --git a/assets/datavzrd/truvari.datavzrd.template.yaml b/assets/datavzrd/truvari.datavzrd.template.yaml index fc85922..c75c2eb 100644 --- a/assets/datavzrd/truvari.datavzrd.template.yaml +++ b/assets/datavzrd/truvari.datavzrd.template.yaml @@ -23,4 +23,3 @@ views: display-mode: normal F1: display-mode: normal -# Tool,TP_base,TP_call,FP,FN,Precision,Recall,F1 diff --git a/assets/datavzrd/wittyer.datavzrd.template.yaml b/assets/datavzrd/wittyer.datavzrd.template.yaml index eb68464..744d11b 100644 --- a/assets/datavzrd/wittyer.datavzrd.template.yaml +++ b/assets/datavzrd/wittyer.datavzrd.template.yaml @@ -17,4 +17,3 @@ views: display-mode: normal FP: display-mode: normal -# Tool,StatsType,TP_base,TP_comp,FP,FN,Precision,Recall,F1 From 4aee93f6882402b640f8a4c927c37a628fcc3744 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 6 Nov 2024 12:56:07 +0000 Subject: [PATCH 190/292] except itruth everything works --- assets/datavzrd/sompy.datavzrd.template.yaml | 18 +- assets/samplesheet.csv | 6 - assets/samplesheet_full.csv | 5 - assets/samplesheet_full_small.csv | 3 + assets/samplesheet_full_sv.csv | 3 + assets/samplesheet_small.csv | 3 + assets/samplesheet_somatic.csv | 7 - assets/samplesheet_somatic_indel.csv | 3 + assets/samplesheet_somatic_snv.csv | 4 + assets/samplesheet_somatic_sv.csv | 3 + assets/samplesheet_sv.csv | 4 + assets/samplesheet_sv_hg37.csv | 3 + assets/schema_input.json | 11 +- conf/modules.config | 101 +++--- conf/test.config | 48 --- conf/test_full.config | 4 +- conf/test_liftover.config | 44 --- conf/test_somatic.config | 39 --- conf/test_stub.config | 2 +- conf/truth.config | 57 ++-- nextflow.config | 18 +- nextflow_schema.json | 102 ++---- .../local/compare_benchmark_results.nf | 77 +++-- subworkflows/local/liftover_vcfs_truth.nf | 4 +- subworkflows/local/prepare_vcfs_test.nf | 33 +- subworkflows/local/prepare_vcfs_truth.nf | 4 +- .../local/report_benchmark_statistics.nf | 2 +- subworkflows/local/report_vcf_statistics.nf | 40 ++- .../local/small_germline_benchmark.nf | 12 +- subworkflows/local/small_somatic_benchmark.nf | 4 +- subworkflows/local/sv_germline_benchmark.nf | 18 +- subworkflows/local/sv_vcf_conversion.nf | 9 +- .../main.nf | 26 +- subworkflows/local/vcf_variant_filtering.nf | 31 +- workflows/variantbenchmarking.nf | 322 ++++-------------- 35 files changed, 357 insertions(+), 713 deletions(-) delete mode 100644 assets/samplesheet.csv delete mode 100644 assets/samplesheet_full.csv create mode 100644 assets/samplesheet_full_small.csv create mode 100644 assets/samplesheet_full_sv.csv create mode 100644 assets/samplesheet_small.csv delete mode 100644 assets/samplesheet_somatic.csv create mode 100644 assets/samplesheet_somatic_indel.csv create mode 100644 assets/samplesheet_somatic_snv.csv create mode 100644 assets/samplesheet_somatic_sv.csv create mode 100644 assets/samplesheet_sv.csv create mode 100644 assets/samplesheet_sv_hg37.csv delete mode 100644 conf/test.config delete mode 100644 conf/test_liftover.config delete mode 100644 conf/test_somatic.config diff --git a/assets/datavzrd/sompy.datavzrd.template.yaml b/assets/datavzrd/sompy.datavzrd.template.yaml index ac3eaf6..dc5afea 100644 --- a/assets/datavzrd/sompy.datavzrd.template.yaml +++ b/assets/datavzrd/sompy.datavzrd.template.yaml @@ -9,10 +9,12 @@ views: columns: Tool: display-mode: normal - Threshold: + Type: display-mode: normal TP_base: display-mode: normal + TP: + display-mode: normal FN: display-mode: normal TP_call: @@ -23,5 +25,17 @@ views: display-mode: normal Recall: display-mode: normal - F1: + recall_lower: + display-mode: normal + recall_upper: + display-mode: normal + recall2: + display-mode: normal + precision_lower: + display-mode: normal + precision_upper: + display-mode: normal + fp.region.size: + display-mode: normal + fp.rate: display-mode: normal diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv deleted file mode 100644 index af2c0cd..0000000 --- a/assets/samplesheet.csv +++ /dev/null @@ -1,6 +0,0 @@ -id,test_vcf,caller,vartype,pctsize,pctseq,pctovl,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,evaluationmode -manta1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -merged1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -dragen1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,sv,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -strelka1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.strelka.variants.chr21.vcf.gz,strelka,small,,,,,,,,,,,, -bcftools1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.bcftools.chr21.vcf.gz,bcftools,small,,,,,,,,,,,, diff --git a/assets/samplesheet_full.csv b/assets/samplesheet_full.csv deleted file mode 100644 index ca2a6b1..0000000 --- a/assets/samplesheet_full.csv +++ /dev/null @@ -1,5 +0,0 @@ -id,test_vcf,caller,vartype,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode,subsample -test1,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/manta_GIABv0.6/diploidSV_PASS_DUPtoINS.vcf.gz",manta,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, -test2,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/delly_GIABv0.6/norm_recall_HG002.merged__filt_DUPtoINS.vcf.gz",delly,sv,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, -test4,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/SevenBridges_GraphGATKRefine_05052017/HG002-NA24385-50x.union_170414.split.vcf.gz",graph,small,,,,,,,,,,,,, -test5,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/OsloUniversityHospital_Exome_GATK_jointVC_11242015/HG002-HG003-HG004.jointVC.filter.vcf",gatk,small,,,,,,,,,,,,,"Sample_Diag-excap51-HG002-EEogPU" diff --git a/assets/samplesheet_full_small.csv b/assets/samplesheet_full_small.csv new file mode 100644 index 0000000..d525fa4 --- /dev/null +++ b/assets/samplesheet_full_small.csv @@ -0,0 +1,3 @@ +id,test_vcf,caller,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode,subsample +test1,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/SevenBridges_GraphGATKRefine_05052017/HG002-NA24385-50x.union_170414.split.vcf.gz",graph,,,,,,,,,,,,, +test2,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/OsloUniversityHospital_Exome_GATK_jointVC_11242015/HG002-HG003-HG004.jointVC.filter.vcf",gatk,,,,,,,,,,,,,"Sample_Diag-excap51-HG002-EEogPU" diff --git a/assets/samplesheet_full_sv.csv b/assets/samplesheet_full_sv.csv new file mode 100644 index 0000000..ca2bb05 --- /dev/null +++ b/assets/samplesheet_full_sv.csv @@ -0,0 +1,3 @@ +id,test_vcf,caller,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode,subsample +test1,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/manta_GIABv0.6/diploidSV_PASS_DUPtoINS.vcf.gz",manta,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, +test2,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/delly_GIABv0.6/norm_recall_HG002.merged__filt_DUPtoINS.vcf.gz",delly,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, diff --git a/assets/samplesheet_small.csv b/assets/samplesheet_small.csv new file mode 100644 index 0000000..77934d2 --- /dev/null +++ b/assets/samplesheet_small.csv @@ -0,0 +1,3 @@ +id,test_vcf,caller +test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.strelka.variants.chr21.vcf.gz,strelka +test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.bcftools.chr21.vcf.gz,bcftools diff --git a/assets/samplesheet_somatic.csv b/assets/samplesheet_somatic.csv deleted file mode 100644 index 161565f..0000000 --- a/assets/samplesheet_somatic.csv +++ /dev/null @@ -1,7 +0,0 @@ -id,test_vcf,caller,vartype,subsample -freebayes,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.freebayes.chr21.vcf.gz,freebayes,small, -manta1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.manta.somatic_sv.vcf.gz,manta,snv,"HCC1395_HCC1395T" -strelka1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_indels.vcf.gz,strelka,indel,"TUMOR" -strelka2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.vcf.gz,strelka,snv,"TUMOR" -tiddit2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.tiddit_sv_merge.vcf.gz,tiddit,sv,"HCC1395_HCC1395T" -manta2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.manta.somatic_sv.vcf.gz,manta,sv,"HCC1395_HCC1395T" diff --git a/assets/samplesheet_somatic_indel.csv b/assets/samplesheet_somatic_indel.csv new file mode 100644 index 0000000..7792ae3 --- /dev/null +++ b/assets/samplesheet_somatic_indel.csv @@ -0,0 +1,3 @@ +id,test_vcf,caller,subsample +test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.freebayes.chr21.vcf.gz,freebayes, +test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_indels.vcf.gz,strelka,"TUMOR" diff --git a/assets/samplesheet_somatic_snv.csv b/assets/samplesheet_somatic_snv.csv new file mode 100644 index 0000000..07708b9 --- /dev/null +++ b/assets/samplesheet_somatic_snv.csv @@ -0,0 +1,4 @@ +id,test_vcf,caller,subsample +test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.freebayes.chr21.vcf.gz,freebayes, +test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.manta.somatic_sv.vcf.gz,manta,"HCC1395_HCC1395T" +test3,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.vcf.gz,strelka,"TUMOR" diff --git a/assets/samplesheet_somatic_sv.csv b/assets/samplesheet_somatic_sv.csv new file mode 100644 index 0000000..7aeae5a --- /dev/null +++ b/assets/samplesheet_somatic_sv.csv @@ -0,0 +1,3 @@ +id,test_vcf,caller,subsample +test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.tiddit_sv_merge.vcf.gz,tiddit,"HCC1395_HCC1395T" +test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.manta.somatic_sv.vcf.gz,manta,"HCC1395_HCC1395T" diff --git a/assets/samplesheet_sv.csv b/assets/samplesheet_sv.csv new file mode 100644 index 0000000..dd76036 --- /dev/null +++ b/assets/samplesheet_sv.csv @@ -0,0 +1,4 @@ +id,test_vcf,caller,pctsize,pctseq,pctovl,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,evaluationmode +test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts +test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts +test3,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts diff --git a/assets/samplesheet_sv_hg37.csv b/assets/samplesheet_sv_hg37.csv new file mode 100644 index 0000000..2089199 --- /dev/null +++ b/assets/samplesheet_sv_hg37.csv @@ -0,0 +1,3 @@ +id,test_vcf,caller +test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_delly_SV_hg19.chr21.vcf.gz,delly +test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_manta_SV_hg19_genotype.chr21.vcf.gz,manta diff --git a/assets/schema_input.json b/assets/schema_input.json index fd84111..7967366 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -31,15 +31,6 @@ "minLength": 1, "errorMessage": "Variant caller has to be defined. Can also be unknown, undefined or merged" }, - "vartype": { - "type": "string", - "pattern": "^\\S+$", - "description": "Variant type to apply benchmarking", - "meta": ["vartype"], - "minLength": 1, - "errorMessage": "Variant type can be only one of these: small, sv, snv, indel and cnv", - "enum": ["small", "sv", "snv", "indel", "cnv"] - }, "subsample": { "type": "string", "pattern": "^\\S+$", @@ -165,6 +156,6 @@ "default": null } }, - "required": ["test_vcf", "caller", "vartype", "id"] + "required": ["test_vcf", "caller", "id"] } } diff --git a/conf/modules.config b/conf/modules.config index cbd9898..d5ff443 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -20,7 +20,7 @@ process { withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ - path: { "${params.outdir}/multiqc" }, + path: { "${params.outdir}/${params.variant_type}/multiqc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -33,15 +33,15 @@ process { withName: "VARIANT_EXTRACTOR" { ext.prefix = { input.baseName - ".vcf" } publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz}", mode: params.publish_dir_mode ] } withName: SVYNC { - ext.prefix = {"${meta.id}.${meta.vartype}.${meta.caller}"} + ext.prefix = {"${meta.id}.${meta.caller}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", mode: params.publish_dir_mode ] @@ -54,7 +54,7 @@ process { ext.prefix = { vcf.baseName - ".vcf" + ".sort"} ext.args = {"--output-type z" } publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz}", mode: params.publish_dir_mode ] @@ -78,7 +78,7 @@ process { ext.prefix = { vcf.baseName - ".vcf" + ".snv" } ext.args = {"--output-type v --types snps" } publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf}", mode: params.publish_dir_mode ] @@ -87,7 +87,7 @@ process { ext.prefix = { vcf.baseName - ".vcf" + ".indel" } ext.args = {"--output-type v --types indels" } publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf}", mode: params.publish_dir_mode ] @@ -101,7 +101,7 @@ process { (params.exclude_expression != null )? "--exclude '$params.exclude_expression'" : "" ].join(' ').trim() } publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess/"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess/"}, pattern: "*{.vcf}", mode: params.publish_dir_mode ] @@ -109,7 +109,7 @@ process { withName: SURVIVOR_FILTER { ext.prefix = { vcf_file.baseName - ".vcf" + ".filter"} publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf}", mode: params.publish_dir_mode ] @@ -135,30 +135,29 @@ process { } // Variant stats withName: SURVIVOR_STATS { - ext.prefix ={["${meta.id}.${meta.vartype}", + ext.prefix ={["${meta.id}", (meta.caller != null) ? ".${meta.caller}" : '' ].join('').trim() } publishDir = [ - path: {"${params.outdir}/${meta.id}/stats/survivor/"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/stats/survivor/"}, pattern: "*{.stats}", mode: params.publish_dir_mode ] } withName: BCFTOOLS_STATS { - ext.prefix ={["${meta.id}.${meta.vartype}", + ext.prefix ={["${meta.id}", (meta.caller != null) ? ".${meta.caller}" : '' ].join('').trim() } publishDir = [ - path: {"${params.outdir}/${meta.id}/stats/bcftools/"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/stats/bcftools/"}, pattern: "*{stats.txt}", mode: params.publish_dir_mode ] } // benchmark tools withName: "RTGTOOLS_FORMAT" { - ext.when = { params.method.split(',').contains('rtgtools') && !params.sdf } publishDir = [ path: {"${params.outdir}/references/rtgtools"}, pattern: "*{.sdf}", @@ -166,44 +165,43 @@ process { ] } withName: "RTGTOOLS_VCFEVAL" { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} //ext.args = {"--ref-overlap --all-record --output-mode ga4gh"} - ext.when = { params.method.split(',').contains('rtgtools') } publishDir = [ - path: {"${params.outdir}/${meta.id}/rtgtools_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/rtgtools"}, pattern: "*{.vcf.gz,vcf.gz.tbi,tsv.gz,txt}", mode: params.publish_dir_mode ] } withName: "HAPPY_HAPPY" { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} //ext.args = {""} publishDir = [ - path: {"${params.outdir}/${meta.id}/happy_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/happy"}, pattern: "*{.csv.gz,csv,json.gz,json,vcf.gz,vcf.gz.tbi}", mode: params.publish_dir_mode ] } withName: "HAPPY_SOMPY" { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} - ext.args = { meta.caller.contains("strelka") || meta.caller.contains("varscan") || meta.caller.contains("pisces") ? "--feature-table hcc.${meta.caller}.${meta.vartype} --bin-afs" : "--feature-table generic" } + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} + ext.args = { meta.caller.contains("strelka") || meta.caller.contains("varscan") || meta.caller.contains("pisces") ? "--feature-table hcc.${meta.caller}.${params.variant_type} --bin-afs" : "--feature-table generic" } publishDir = [ - path: {"${params.outdir}/${meta.id}/sompy_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/sompy"}, pattern: "*{.csv.gz,csv,json.gz,json,vcf.gz,vcf.gz.tbi,csv}", mode: params.publish_dir_mode ] } withName: "HAPPY_PREPY" { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = {"--fixchr --filter-nonref --bcftools-norm"} publishDir = [ - path: {"${params.outdir}/${meta.id}/preprocess"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{vcf.gz}", mode: params.publish_dir_mode ] } withName: "TRUVARI_BENCH" { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = {[ "--sizemin 0 --sizefilt 0 --sizemax 5000000", (meta.pctseq != null) ? "--pctsize ${meta.pctsize}" : '', @@ -214,30 +212,28 @@ process { (meta.dup_to_ins) ? "--dup-to-ins" : '', (meta.typeignore) ? "--typeignore" : '' ].join(' ').trim()} - ext.when = { params.method.split(',').contains('truvari') } publishDir = [ - path: {"${params.outdir}/${meta.id}/truvari_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/truvari"}, pattern: "*{.vcf.gz,vcf.gz.tbi,json}", mode: params.publish_dir_mode ] } withName: SVANALYZER_SVBENCHMARK { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = {[ (meta.normshift != null) ? "-normshift ${meta.normshift}" : '', (meta.normdist != null) ? "-normdist ${meta.normdist}" : '', (meta.normsizediff != null) ? "-normsizediff ${meta.normsizediff}" : '', (meta.maxdist != null) ? "-maxdist ${meta.maxdist}" : '' ].join(' ').trim()} - ext.when = { params.method.split(',').contains('svanalyzer') } publishDir = [ - path: {"${params.outdir}/${meta.id}/svanalyzer_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/svanalyzer"}, pattern: "*{.vcf.gz,tbi,distances,log,report}", mode: params.publish_dir_mode ] } withName: WITTYER { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}.${meta.caller}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = {[ "--includedFilters=''", (meta.evaluationmode != null) ? "-em ${meta.evaluationmode}" : '', @@ -247,32 +243,32 @@ process { (meta.maxMatches != null) ? "--mm ${meta.maxMatches}" : '' ].join(' ').trim()} publishDir = [ - path: {"${params.outdir}/${meta.id}/wittyer_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/wittyer_bench"}, pattern: "*{.vcf.gz,tbi,json}", mode: params.publish_dir_mode ] } withName: BAMSURGEON_EVALUATOR { - ext.prefix = {"${meta.id}.${params.sample}.${meta.vartype}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} publishDir = [ - path: {"${params.outdir}/${meta.id}/bamsurgeon_evalator"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/bamsurgeon"}, pattern: "*{.vcf,stats}", mode: params.publish_dir_mode ] } // summary reports withName: MERGE_REPORTS { - ext.prefix = {"${meta.benchmark_tool}.${meta.vartype}"} + ext.prefix = {"${meta.benchmark_tool}"} publishDir = [ - path: {"${params.outdir}/summary/tables/${meta.vartype}/${meta.benchmark_tool}"}, + path: {"${params.outdir}/${params.variant_type}/summary/tables/${meta.benchmark_tool}"}, pattern: "*{csv}", mode: params.publish_dir_mode ] } withName: PLOTS { - ext.prefix = {"${meta.benchmark_tool}.${meta.vartype}"} + ext.prefix = {"${meta.benchmark_tool}"} publishDir = [ - path: {"${params.outdir}/summary/plots/${meta.vartype}/${meta.benchmark_tool}"}, + path: {"${params.outdir}/${params.variant_type}/summary/plots/${meta.benchmark_tool}"}, pattern: "*{png}", mode: params.publish_dir_mode ] @@ -280,7 +276,7 @@ process { withName: DATAVZRD { ext.prefix = {"${meta.id}"} publishDir = [ - path: {"${params.outdir}/summary/datavzrd/${meta.vartype}"}, + path: {"${params.outdir}/${params.variant_type}/summary/datavzrd/"}, pattern: "*", mode: params.publish_dir_mode ] @@ -290,29 +286,28 @@ process { ext.prefix = {input.toString() - ".vcf.gz"} } withName: SURVIVOR_MERGE { - ext.prefix = {"${meta.id}.${meta.vartype}.${meta.tag}"} + ext.prefix = {"${meta.id}.${meta.tag}"} } withName: BCFTOOLS_MERGE { - ext.prefix = {"${meta.id}.${meta.vartype}.${meta.tag}"} + ext.prefix = {"${meta.id}.${meta.tag}"} ext.args = {"--output-type v --force-samples --force-single"} } withName: VCF_TO_CSV { - ext.prefix = {"${meta.id}.${meta.vartype}.${meta.tag}"} + ext.prefix = {"${meta.id}.${meta.tag}"} publishDir = [ - path: {"${params.outdir}/summary/comparisons/${meta.vartype}"}, + path: {"${params.outdir}/${params.variant_type}/summary/comparisons/${meta.vartype}"}, pattern: "*{.csv}", mode: params.publish_dir_mode ] } withName: REFORMAT_HEADER { - ext.prefix ={["${meta.id}.${meta.vartype}", + ext.prefix ={["${meta.id}", (meta.tag != null) ? ".${meta.tag}" : '' ].join('').trim() } } // liftOver withName: PICARD_CREATESEQUENCEDICTIONARY { - ext.when = { params.liftover } publishDir = [ path: {"${params.outdir}/references/dictionary"}, pattern: "*{.dict}", @@ -320,32 +315,28 @@ process { ] } withName: PICARD_LIFTOVERVCF { - ext.prefix = {"${meta.id}.${meta.vartype}"} + ext.prefix = {"${meta.id}}"} ext.args = {"--WARN_ON_MISSING_CONTIG true"} - ext.when = { params.liftover } } withName: BCFTOOLS_RENAME_CHR { - ext.prefix = {"${meta.id}.${meta.vartype}.renamechr"} + ext.prefix = {"${meta.id}.renamechr"} ext.args = {"--output-type z"} - ext.when = { params.liftover } publishDir = [ - path: {"${params.outdir}/${params.sample}/liftover"}, + path: {"${params.outdir}/${params.truth_id}/liftover"}, pattern: "*{.vcf.gz}", mode: params.publish_dir_mode ] } withName: UCSC_LIFTOVER { - ext.prefix = {"${meta.id}.${meta.vartype}.liftover"} - ext.when = { params.liftover } + ext.prefix = {"${meta.id}.liftover"} } withName: SORT_BED { - ext.prefix = {"${meta.id}.${meta.vartype}.sort"} - ext.when = { params.liftover } + ext.prefix = {"${meta.id}.sort"} } withName: BEDTOOLS_MERGE { ext.prefix = {bed.toString() - ".bed" + ".merged" } publishDir = [ - path: {"${params.outdir}/${params.sample}/liftover"}, + path: {"${params.outdir}/${params.variant_type}/${params.truth_id}/liftover"}, pattern: "*{.bed}", mode: params.publish_dir_mode ] diff --git a/conf/test.config b/conf/test.config deleted file mode 100644 index c6518d2..0000000 --- a/conf/test.config +++ /dev/null @@ -1,48 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, --outdir - ----------------------------------------------------------------------------------------- -*/ - -process { - resourceLimits = [ - cpus: 4, - memory: '15.GB', - time: '1.h' - ] -} - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Input data - sample = "HG002" - input = "assets/samplesheet.csv" - outdir = "results" - - // Genome references - genome = 'GRCh38' - - // Processes - analysis = 'germline' - method = 'happy,truvari,svanalyzer,wittyer,rtgtools' // - preprocess = "normalization,deduplication,prepy" - sv_standardization = "svync,homogenize" - - include_expression = 'FILTER="."' - min_sv_size = 30 - - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" - - itruth_ignore = true -} diff --git a/conf/test_full.config b/conf/test_full.config index 71ad921..dd92cdd 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -20,13 +20,13 @@ params { max_time = '8.h' // Input data - input = 'assets/samplesheet_full.csv' + input = null outdir = 'results' // Genome references genome = 'GRCh37' analysis = 'germline' - sample = "HG002" + truth_id = "HG002" method = 'truvari,svanalyzer,happy,rtgtools' diff --git a/conf/test_liftover.config b/conf/test_liftover.config deleted file mode 100644 index db3dee4..0000000 --- a/conf/test_liftover.config +++ /dev/null @@ -1,44 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/benchmark -profile test_liftover, --outdir - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.5GB' - max_time = '8.h' - - // Input data - input = 'assets/samplesheet.csv' - outdir = 'results' - genome = 'GRCh38' - - // Processes - analysis = 'germline' - sample = "HG002" - method = 'truvari,svanalyzer,rtgtools,happy' - preprocess = "normalization,deduplication,filter_contigs" - min_sv_size = 30 - - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" - itruth_ignore = true - - //liftover files - chain = "https://raw.githubusercontent.com/broadinstitute/gatk/master/scripts/funcotator/data_sources/gnomAD/b37ToHg38.over.chain" - rename_chr = "assets/rename_contigs/grch37_grch38.txt" - liftover = true -} diff --git a/conf/test_somatic.config b/conf/test_somatic.config deleted file mode 100644 index 345b68e..0000000 --- a/conf/test_somatic.config +++ /dev/null @@ -1,39 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir - ----------------------------------------------------------------------------------------- -*/ - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Limit resources so that this can run on GitHub Actions - max_cpus = 2 - max_memory = '6.5GB' - max_time = '8.h' - - // Input data - input = 'assets/samplesheet_somatic.csv' - outdir = 'results' - - // Genome references - genome = 'GRCh38' - analysis = 'somatic' - sample = "SEQC2" - method = 'sompy,truvari' - preprocess = "filter_contigs" - - truth_snv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sSNV_truth_set_v1.0.chr21.vcf.gz" - high_conf_snv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" - truth_indel = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sINDEL_truth_set_v1.0.chr21.vcf.gz" - high_conf_indel = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/13059_2022_2816_MOESM4_ESM.vcf.gz" - itruth_ignore = true -} diff --git a/conf/test_stub.config b/conf/test_stub.config index fdbf8b8..8ecbb74 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -21,7 +21,7 @@ params { // Input data sample = "HG002" - input = "assets/samplesheet_somatic.csv" + input = "assets/samplesheet_sv.csv" outdir = "results" // Genome references diff --git a/conf/truth.config b/conf/truth.config index cda4ac1..6ed1b21 100644 --- a/conf/truth.config +++ b/conf/truth.config @@ -12,35 +12,52 @@ params { // Base directory for truth data germline { 'GRCh38' { - 'HG002'{ - truth_small = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh38/HG002_GRCh38_1_22_v4.2.1_benchmark.vcf.gz" - high_conf_small = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh38/HG002_GRCh38_1_22_v4.2.1_benchmark_noinconsistent.bed" - truth_sv = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/CMRG_v1.00/GRCh38/StructuralVariant/HG002_GRCh38_CMRG_SV_v1.00.vcf.gz" - high_conf_sv = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/CMRG_v1.00/GRCh38/StructuralVariant/HG002_GRCh38_CMRG_SV_v1.00.bed" - chain = "http://ftp.ensembl.org/pub/assembly_mapping/homo_sapiens/GRCh38_to_GRCh37.chain.gz" - rename_chr = "assets/rename_contigs/grch38_grch37.txt" + "small" { + 'HG002'{ + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh38/HG002_GRCh38_1_22_v4.2.1_benchmark.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh38/HG002_GRCh38_1_22_v4.2.1_benchmark_noinconsistent.bed" + } } + "somatic" { + 'HG002'{ + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/CMRG_v1.00/GRCh38/StructuralVariant/HG002_GRCh38_CMRG_SV_v1.00.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/CMRG_v1.00/GRCh38/StructuralVariant/HG002_GRCh38_CMRG_SV_v1.00.bed" + } + } + } 'GRCh37'{ - 'HG002'{ - truth_small = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.vcf.gz" - high_conf_small = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.bed" - truth_sv = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/HG002_SVs_Tier1_v0.6.vcf.gz" - high_conf_sv = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/HG002_SVs_Tier1_v0.6.bed" - chain = "https://raw.githubusercontent.com/broadinstitute/gatk/master/scripts/funcotator/data_sources/gnomAD/b37ToHg38.over.chain" - rename_chr = "assets/rename_contigs/grch37_grch38.txt" + "small" { + 'HG002'{ + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.bed" + } + + } + "structural" { + 'HG002'{ + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/HG002_SVs_Tier1_v0.6.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/HG002_SVs_Tier1_v0.6.bed" + } } + } } somatic{ 'GRCh38'{ - 'SEQC2'{ - truth_snv = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/high-confidence_sSNV_in_HC_regions_v1.2.1.vcf.gz" - high_conf_snv = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/High-Confidence_Regions_v1.2.bed" - truth_indel = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/high-confidence_sINDEL_in_HC_regions_v1.2.1.vcf.gz" - high_conf_indel = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/High-Confidence_Regions_v1.2.bed" - + "snv"{ + 'SEQC2'{ + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/high-confidence_sSNV_in_HC_regions_v1.2.1.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/High-Confidence_Regions_v1.2.bed" + } } + "indel" { + 'SEQC2'{ + truth_indel = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/high-confidence_sINDEL_in_HC_regions_v1.2.1.vcf.gz" + high_conf_indel = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/High-Confidence_Regions_v1.2.bed" + } + } + } } } diff --git a/nextflow.config b/nextflow.config index 8736a95..f8484a8 100644 --- a/nextflow.config +++ b/nextflow.config @@ -9,22 +9,23 @@ // Global default params, used in configs params { - // TODO nf-core: Specify your pipeline's command line flags // Input options input = null outdir = "results" - sample = null + + // Option to select analysis type: germline or somatic analysis = null - sdf = null - dictionary = null - liftover = false + + // Option to select variant type to analysis: small, snv, indel, somatic, copynumber + variant_type = null + + // Truth parameters + truth_id = null // Benchmarking method method = 'truvari,svanalyzer,happy,rtgtools,wittyer' // Preprocess spesific parameters - // normalization includes braking down multiallelic samples -m any - // deduplication removes one of the variants in the same position preprocess = "" sv_standardization = "" @@ -41,6 +42,9 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false + sdf = null + dictionary = null + liftover = false // Truth library itruth_ignore = false diff --git a/nextflow_schema.json b/nextflow_schema.json index 51ed763..8ff5b2b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], + "required": ["input", "outdir", "truth_id", "analysis", "variant_type", "method", "truth_vcf"], "properties": { "input": { "type": "string", @@ -29,9 +29,9 @@ "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", "fa_icon": "fas fa-folder-open" }, - "sample": { + "truth_id": { "type": "string", - "description": "Sample id", + "description": "Truth id, sample name to define truth vcf", "fa_icon": "fas fa-folder-open", "errorMessage": "The sample name of the truth case. Examples: HG002, SEQC2, HG001, HG003, CHM13" }, @@ -39,6 +39,16 @@ "type": "string", "description": "The analysis type used by the input files", "enum": ["germline", "somatic"], + "pattern": "(germline|somatic)", + "fa_icon": "fas fa-folder-open", + "errorMessage": "Analysis type has to be choosen: germline or somatic" + }, + "variant_type": { + "type": "string", + "description": "Variant types to benchmark", + "errorMessage": "Select a variant type to make the analysis: small,snv,indel,structural or copynumber. Select small when your vcf contains both snvs and indels", + "enum": ["small", "snv", "indel", "structural", "copynumber"], + "pattern": "(small|snv|indel|structural|copynumber)", "fa_icon": "fas fa-folder-open" }, "method": { @@ -48,96 +58,24 @@ "pattern": "^((truvari|svanalyzer|happy|sompy|rtgtools|wittyer)?,?)*(? tuple([id: params.truth_id], file)}, chain.map{meta, file -> file} ) versions = versions.mix(UCSC_LIFTOVER.out.versions.first()) diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 45f3be1..e1a6f3f 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -88,22 +88,25 @@ workflow PREPARE_VCFS_TEST { if (params.analysis.contains("somatic")){ // somatic spesific preperations - vcf_ch.branch{ - def meta = it[0] - small: meta.vartype == "small" - other: true - } - .set{vcf} + //vcf_ch.branch{ + // def meta = it[0] + // small: meta.vartype == "small" + // other: true + // } + // .set{vcf} + + if (params.variant_type == "small"){ + out_vcf_ch = Channel.empty() + + SPLIT_SMALL_VARIANTS_TEST( + vcf.small + ) + versions = versions.mix(SPLIT_SMALL_VARIANTS_TEST.out.versions.first()) + out_vcf_ch = out_vcf_ch.mix(SPLIT_SMALL_VARIANTS_TEST.out.out_vcf_ch, + vcf.other) + vcf_ch = out_vcf_ch + } - out_vcf_ch = Channel.empty() - - SPLIT_SMALL_VARIANTS_TEST( - vcf.small - ) - versions = versions.mix(SPLIT_SMALL_VARIANTS_TEST.out.versions.first()) - out_vcf_ch = out_vcf_ch.mix(SPLIT_SMALL_VARIANTS_TEST.out.out_vcf_ch, - vcf.other) - vcf_ch = out_vcf_ch } emit: diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 6278699..4db015f 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -13,7 +13,7 @@ include { LIFTOVER_VCFS_TRUTH } from '../local/liftover_vcfs_truth' workflow PREPARE_VCFS_TRUTH { take: truth_ch // channel: [val(meta), vcf] - high_conf_ch // channel: [val(meta), bed] + high_conf_ch // channel: [bed] fasta // reference channel [val(meta), ref.fa] fai // reference channel [val(meta), ref.fa.fai] chain // reference channel [val(meta), chain.gz] @@ -37,7 +37,7 @@ workflow PREPARE_VCFS_TRUTH { ) versions = versions.mix(LIFTOVER_VCFS_TRUTH.out.versions.first()) truth_ch = LIFTOVER_VCFS_TRUTH.out.vcf_ch - high_conf_ch = LIFTOVER_VCFS_TRUTH.out.bed_ch + high_conf_ch = LIFTOVER_VCFS_TRUTH.out.bed_ch.map{ meta, bed -> [bed]} } // Reheader sample name for truth file - using meta.caller diff --git a/subworkflows/local/report_benchmark_statistics.nf b/subworkflows/local/report_benchmark_statistics.nf index 8ed96dd..a58afed 100644 --- a/subworkflows/local/report_benchmark_statistics.nf +++ b/subworkflows/local/report_benchmark_statistics.nf @@ -27,7 +27,7 @@ workflow REPORT_BENCHMARK_STATISTICS { versions = versions.mix(PLOTS.out.versions.first()) MERGE_REPORTS.out.summary - .map { meta, file -> tuple([vartype: meta.vartype] + [id: meta.benchmark_tool], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [id: meta.benchmark_tool], file) } .set { summary } // add path to csv file to the datavzrd input diff --git a/subworkflows/local/report_vcf_statistics.nf b/subworkflows/local/report_vcf_statistics.nf index 3a3423c..b6c283a 100644 --- a/subworkflows/local/report_vcf_statistics.nf +++ b/subworkflows/local/report_vcf_statistics.nf @@ -12,25 +12,29 @@ workflow REPORT_VCF_STATISTICS { main: versions = Channel.empty() + survivor_stats = Channel.empty() + + //input_ch.branch{ + // def meta = it[0] + // sv: meta.vartype == "sv" || meta.vartype == "cnv" + // other: true + // } + // .set{input} + + if (params.variant_type == "structural"){ + // use survivor stats to get SV statistics by TYPE + SURVIVOR_STATS( + input_ch.map{ meta, vcf, tbi -> + [ meta, vcf ] + }, + -1, + -1, + -1 + ) + survivor_stats = SURVIVOR_STATS.out.stats + versions = versions.mix(SURVIVOR_STATS.out.versions.first()) + } - input_ch.branch{ - def meta = it[0] - sv: meta.vartype == "sv" || meta.vartype == "cnv" - other: true - } - .set{input} - - // use survivor stats to get SV statistics by TYPE - SURVIVOR_STATS( - input.sv.map{ meta, vcf, tbi -> - [ meta, vcf ] - }, - -1, - -1, - -1 - ) - survivor_stats = SURVIVOR_STATS.out.stats - versions = versions.mix(SURVIVOR_STATS.out.versions.first()) // use bcftools stats for all files BCFTOOLS_STATS( diff --git a/subworkflows/local/small_germline_benchmark.nf b/subworkflows/local/small_germline_benchmark.nf index 7bfd346..a74ee9c 100644 --- a/subworkflows/local/small_germline_benchmark.nf +++ b/subworkflows/local/small_germline_benchmark.nf @@ -48,7 +48,7 @@ workflow SMALL_GERMLINE_BENCHMARK { // collect summary reports RTGTOOLS_VCFEVAL.out.summary - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "rtgtools"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "rtgtools"], file) } .groupTuple() .set{ report } @@ -62,7 +62,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_1.out.versions) VCF_REHEADER_SAMPLENAME_1.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "FN"] + [id: "rtgtools"], file, index) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "rtgtools"], file, index) } .set { vcf_fn } VCF_REHEADER_SAMPLENAME_2( @@ -72,7 +72,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_2.out.versions) VCF_REHEADER_SAMPLENAME_2.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "FP"] + [id: "rtgtools"], file, index) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "rtgtools"], file, index) } .set { vcf_fp } VCF_REHEADER_SAMPLENAME_3( @@ -82,7 +82,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_3.out.versions) VCF_REHEADER_SAMPLENAME_3.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "TP_base"] + [id: "rtgtools"], file, index) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "rtgtools"], file, index) } .set { vcf_tp_base } VCF_REHEADER_SAMPLENAME_4( @@ -92,7 +92,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_4.out.versions) VCF_REHEADER_SAMPLENAME_4.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "TP_comp"] + [id: "rtgtools"], file, index) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "rtgtools"], file, index) } .set { vcf_tp_comp } tagged_variants = tagged_variants.mix( @@ -146,7 +146,7 @@ workflow SMALL_GERMLINE_BENCHMARK { // tag meta and collect summary reports HAPPY_HAPPY.out.summary_csv - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "happy"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "happy"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) diff --git a/subworkflows/local/small_somatic_benchmark.nf b/subworkflows/local/small_somatic_benchmark.nf index cfd2e99..78fa535 100644 --- a/subworkflows/local/small_somatic_benchmark.nf +++ b/subworkflows/local/small_somatic_benchmark.nf @@ -31,7 +31,7 @@ workflow SMALL_SOMATIC_BENCHMARK { versions = versions.mix(HAPPY_SOMPY.out.versions.first()) HAPPY_SOMPY.out.stats - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "sompy"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "sompy"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) @@ -50,7 +50,7 @@ workflow SMALL_SOMATIC_BENCHMARK { versions = versions.mix(BAMSURGEON_EVALUATOR.out.versions.first()) BAMSURGEON_EVALUATOR.out.stats - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "bamsurgeon"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "bamsurgeon"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index f67e2d1..56fa2a3 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -36,7 +36,7 @@ workflow SV_GERMLINE_BENCHMARK { versions = versions.mix(TRUVARI_BENCH.out.versions.first()) TRUVARI_BENCH.out.summary - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "truvari"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "truvari"], file) } .groupTuple() .set { report } @@ -50,7 +50,7 @@ workflow SV_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_1.out.versions) VCF_REHEADER_SAMPLENAME_1.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "FN"] + [id: "truvari"], file) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "truvari"], file) } .set { vcf_fn } // reheader fp vcf files for tagged results @@ -62,7 +62,7 @@ workflow SV_GERMLINE_BENCHMARK { // add tag and to meta VCF_REHEADER_SAMPLENAME_2.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "FP"] + [id: "truvari"], file) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "truvari"], file) } .set { vcf_fp } // reheader base tp vcf files for tagged results @@ -74,7 +74,7 @@ workflow SV_GERMLINE_BENCHMARK { // add tag and to meta VCF_REHEADER_SAMPLENAME_3.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "TP_base"] + [id: "truvari"], file) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "truvari"], file) } .set { vcf_tp_base } // reheader comp tp vcf files for tagged results @@ -86,7 +86,7 @@ workflow SV_GERMLINE_BENCHMARK { // add tag and to meta VCF_REHEADER_SAMPLENAME_4.out.ch_vcf - .map { meta, file, index -> tuple([vartype: meta.vartype] + [tag: "TP_comp"] + [id: "truvari"], file) } + .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "truvari"], file) } .set { vcf_tp_comp } // collect tagged variant files @@ -108,7 +108,7 @@ workflow SV_GERMLINE_BENCHMARK { // tag and collect summary file SVANALYZER_SVBENCHMARK.out.report - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "svbenchmark"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "svbenchmark"], file) } .groupTuple() .set{ report } @@ -116,12 +116,12 @@ workflow SV_GERMLINE_BENCHMARK { // reheader fn vcf files for tagged results SVANALYZER_SVBENCHMARK.out.fns - .map { meta, file -> tuple([vartype: meta.vartype] + [tag: "FN"] + [id: "svbenchmark"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "svbenchmark"], file) } .set { vcf_fn } SVANALYZER_SVBENCHMARK.out.fps - .map { meta, file -> tuple([vartype: meta.vartype] + [tag: "FP"] + [id: "svbenchmark"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "svbenchmark"], file) } .set { vcf_fp } tagged_variants = tagged_variants.mix( @@ -163,7 +163,7 @@ workflow SV_GERMLINE_BENCHMARK { versions = versions.mix(WITTYER.out.versions.first()) WITTYER.out.report - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "wittyer"], file) } + .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "wittyer"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index ec3717d..c92e214 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -58,7 +58,7 @@ workflow SV_VCF_CONVERSIONS { tool: supported return [ meta, vcf, tbi] other: !supported - return [ meta, vcf, tbi ] + return [ meta, vcf ] } .set{input} @@ -80,16 +80,11 @@ workflow SV_VCF_CONVERSIONS { .map{ def meta = it[0] def vcf = it[1] - def tbi = it[2] - [ meta, vcf, tbi ] + [ meta, vcf ] } .set { vcf_ch } - out_vcf_ch = out_vcf_ch.mix(SVYNC.out.vcf, - input.other) - vcf_ch = out_vcf_ch.map{it -> tuple(it[0], it[1], it[2])} } - emit: vcf_ch versions diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index 56e2038..2336434 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -159,42 +159,42 @@ def getGenomeAttribute(attribute) { // def getTruthAttribute(attribute) { if (!params.itruth_ignore){ - if (params.sample && params.analysis && params.genome) { + if (params.truth_id && params.analysis && params.genome) { if (params.liftover){ if (params.genome == "GRCh38"){ if (params.analysis == "somatic"){ - if (params.somatic[ "GRCh37" ][ params.sample ].containsKey(attribute)){ - return params.somatic[ "GRCh37" ][ params.sample ][ attribute ] + if (params.somatic[ "GRCh37" ][ params.truth_id ].containsKey(attribute)){ + return params.somatic[ "GRCh37" ][ params.truth_id ][ attribute ] } } else if(params.analysis == "germline") { - if (params.germline[ "GRCh37" ][ params.sample ].containsKey(attribute)){ - return params.germline[ "GRCh37" ][ params.sample ][ attribute ] + if (params.germline[ "GRCh37" ][ params.truth_id ].containsKey(attribute)){ + return params.germline[ "GRCh37" ][ params.truth_id ][ attribute ] } } } else if (params.genome == "GRCh37"){ if (params.analysis == "somatic"){ - if (params.somatic[ "GRCh38" ][ params.sample ].containsKey(attribute)){ - return params.somatic[ "GRCh38" ][ params.sample ][ attribute ] + if (params.somatic[ "GRCh38" ][ params.truth_id ].containsKey(attribute)){ + return params.somatic[ "GRCh38" ][ params.truth_id ][ attribute ] } } else if(params.analysis == "germline") { - if (params.germline[ "GRCh38" ][ params.sample ].containsKey(attribute)){ - return params.germline[ "GRCh38" ][ params.sample ][ attribute ] + if (params.germline[ "GRCh38" ][ params.truth_id ].containsKey(attribute)){ + return params.germline[ "GRCh38" ][ params.truth_id ][ attribute ] } } } } else{ if (params.analysis == "somatic"){ - if (params.somatic[ params.genome ][ params.sample ].containsKey(attribute)){ - return params.somatic[ params.genome ][ params.sample ][ attribute ] + if (params.somatic[ params.genome ][ params.truth_id ].containsKey(attribute)){ + return params.somatic[ params.genome ][ params.truth_id ][ attribute ] } } else if(params.analysis == "germline") { - if (params.germline[ params.genome ][ params.sample ].containsKey(attribute)){ - return params.germline[ params.genome][ params.sample ][ attribute ] + if (params.germline[ params.genome ][ params.truth_id ].containsKey(attribute)){ + return params.germline[ params.genome][ params.truth_id ][ attribute ] } } diff --git a/subworkflows/local/vcf_variant_filtering.nf b/subworkflows/local/vcf_variant_filtering.nf index 78c27a2..fda853a 100644 --- a/subworkflows/local/vcf_variant_filtering.nf +++ b/subworkflows/local/vcf_variant_filtering.nf @@ -33,30 +33,21 @@ workflow VCF_VARIANT_FILTERING { vcf_ch = BCFTOOLS_FILTER.out.vcf } - out_vcf_ch = Channel.empty() - if(params.min_sv_size > 0 | params.max_sv_size != -1 | params.min_allele_freq != -1 | params.min_num_reads != -1){ - vcf_ch.branch{ - def meta = it[0] - sv: meta.vartype == "sv" - other: true} - .set{vcf} // filters out smaller SVs than min_sv_size, only applicable to SV files - SURVIVOR_FILTER( - vcf.sv.map{meta, vcf -> tuple( meta, vcf, [])}, - params.min_sv_size, - params.max_sv_size, - params.min_allele_freq, - params.min_num_reads - ) - versions = versions.mix(SURVIVOR_FILTER.out.versions.first()) - - out_vcf_ch = out_vcf_ch.mix(SURVIVOR_FILTER.out.vcf, - vcf.other) - vcf_ch = out_vcf_ch + if (params.variant_type == "structural"){ + SURVIVOR_FILTER( + vcf_ch.map{meta, vcf -> tuple( meta, vcf, [])}, + params.min_sv_size, + params.max_sv_size, + params.min_allele_freq, + params.min_num_reads + ) + versions = versions.mix(SURVIVOR_FILTER.out.versions.first()) + vcf_ch = SURVIVOR_FILTER.out.vcf + } } - // zip and index vcf files TABIX_BGZIPTABIX( vcf_ch diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index eca9072..734bb00 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -54,11 +54,6 @@ workflow VARIANTBENCHMARKING { ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() ch_reports = Channel.empty() - truth_ch = Channel.empty() - high_conf_ch = Channel.empty() - bench_ch = Channel.empty() - sv_evals_ch = Channel.empty() - small_evals_ch = Channel.empty() //// create reference channels //// @@ -67,56 +62,16 @@ workflow VARIANTBENCHMARKING { fai = Channel.fromPath(params.fai, checkIfExists: true) .map{ fai -> tuple([id: fai.getSimpleName()], fai) }.collect() - //// check high confidence files //// + //// check Truth Files //// - // Germline + truth_ch = Channel.fromPath(params.truth_vcf, checkIfExists: true) + .map{ vcf -> tuple([id: params.truth_id, vartype:params.variant_type], vcf) }.collect() - truth_small = params.truth_small ? Channel.fromPath(params.truth_small, checkIfExists: true).map{ vcf -> tuple([id: params.sample, vartype:"small"], vcf) }.collect() + regions_bed_ch = params.regions_bed ? Channel.fromPath(params.regions_bed, checkIfExists: true).collect() : Channel.empty() - truth_ch = truth_ch.mix(truth_small) - - high_conf_small = params.high_conf_small ? Channel.fromPath(params.high_conf_small, checkIfExists: true).map{ bed -> tuple([id: params.sample, vartype:"small"], bed) }.collect() - : Channel.empty() - high_conf_ch = high_conf_ch.mix(high_conf_small) - - truth_sv = params.truth_sv ? Channel.fromPath(params.truth_sv, checkIfExists: true).map{ vcf -> tuple([id: params.sample, vartype:"sv"], vcf) }.collect() - : Channel.empty() - truth_ch = truth_ch.mix(truth_sv) - - high_conf_sv = params.high_conf_sv ? Channel.fromPath(params.high_conf_sv, checkIfExists: true).map{ bed -> tuple([id: params.sample, vartype:"sv"], bed) }.collect() - : Channel.empty() - high_conf_ch = high_conf_ch.mix(high_conf_sv) - - truth_cnv = params.truth_cnv ? Channel.fromPath(params.truth_cnv, checkIfExists: true).map{ vcf -> tuple([id: params.sample, vartype:"cnv"], vcf) }.collect() - : Channel.empty() - truth_ch = truth_ch.mix(truth_cnv) - - high_conf_cnv = params.high_conf_cnv ? Channel.fromPath(params.high_conf_cnv, checkIfExists: true).map{ bed -> tuple([id: params.sample, vartype:"cnv"], bed) }.collect() - : Channel.empty() - high_conf_ch = high_conf_ch.mix(high_conf_cnv) - - // Somatic - // snv and indel seperation only possible for somatic cases - - truth_snv = params.truth_snv ? Channel.fromPath(params.truth_snv, checkIfExists: true).map{ vcf -> tuple([id: params.sample, vartype:"snv"], vcf) }.collect() - : Channel.empty() - truth_ch = truth_ch.mix(truth_snv) - - high_conf_snv = params.high_conf_snv ? Channel.fromPath(params.high_conf_snv, checkIfExists: true).map{ bed -> tuple([id: params.sample, vartype:"snv"], bed) }.collect() - : Channel.empty() - high_conf_ch = high_conf_ch.mix(high_conf_snv) - - truth_indel = params.truth_indel ? Channel.fromPath(params.truth_indel, checkIfExists: true).map{ vcf -> tuple([id: params.sample, vartype:"indel"], vcf) }.collect() - : Channel.empty() - truth_ch = truth_ch.mix(truth_indel) - - high_conf_indel = params.high_conf_indel ? Channel.fromPath(params.high_conf_indel, checkIfExists: true).map{ bed -> tuple([id: params.sample, vartype:"indel"], bed) }.collect() - : Channel.empty() - high_conf_ch = high_conf_ch.mix(high_conf_indel) - // SDF file for RTG-tools eval - sdf = params.sdf ? Channel.fromPath(params.sdf, checkIfExists: true).map{ sdf -> tuple([id: sdf.getSimpleName()], sdf) }.collect() + sdf = params.sdf ? Channel.fromPath(params.sdf, checkIfExists: true).map{ sdf -> tuple([id: sdf.getSimpleName()], sdf) }.collect() : Channel.empty() // read chainfile, liftover genome and rename chr files if liftover is true @@ -145,7 +100,6 @@ workflow VARIANTBENCHMARKING { out_vcf_ch = Channel.empty() - input.multisample.view() SUBSAMPLE_VCF_TEST( input.multisample ) @@ -154,27 +108,20 @@ workflow VARIANTBENCHMARKING { input.other) vcf_ch = out_vcf_ch - // Branch out according to the analysis - vcf_ch.branch{ - sv: it[0].vartype == "sv" - other: true} - .set{test_ch} - - out_vcf_ch = Channel.empty() - - // Standardize SV VCFs, tool spesific modifications - SV_VCF_CONVERSIONS( - test_ch.sv, - fasta, - fai - ) - ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) - out_vcf_ch = out_vcf_ch.mix(SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])}, - test_ch.other) + if (params.variant_type == "structural"){ + // Standardize SV VCFs, tool spesific modifications + SV_VCF_CONVERSIONS( + vcf_ch, + fasta, + fai + ) + ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) + vcf_ch = SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])} + } // Prepare and normalize input vcfs PREPARE_VCFS_TEST( - out_vcf_ch, + vcf_ch, fasta, fai ) @@ -183,14 +130,14 @@ workflow VARIANTBENCHMARKING { // Prepare and normalize truth vcfs PREPARE_VCFS_TRUTH( truth_ch, - high_conf_ch, + regions_bed_ch, fasta, fai, chain, rename_chr, dictionary ) - high_conf_ch = PREPARE_VCFS_TRUTH.out.high_conf_ch + regions_bed_ch = PREPARE_VCFS_TRUTH.out.high_conf_ch ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) // VCF REPORTS AND STATS @@ -201,204 +148,73 @@ workflow VARIANTBENCHMARKING { ) ch_versions = ch_versions.mix(REPORT_VCF_STATISTICS.out.versions) - // branch out input test files - PREPARE_VCFS_TEST.out.vcf_ch.branch{ - def meta = it[0] - sv: meta.vartype == "sv" - small: meta.vartype == "small" - cnv: meta.vartype == "cnv" - snv: meta.vartype == "snv" - indel: meta.vartype == "indel" - other: false - } - .set{test} + // Prepare benchmark channel + PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch) + .combine(regions_bed_ch.ifEmpty([[]])) + .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi, high_bed -> + [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, high_bed ]} + .set{bench} - // branch out truth vcf files - PREPARE_VCFS_TRUTH.out.vcf_ch.branch{ - def meta = it[0] - sv: meta.vartype == "sv" - small: meta.vartype == "small" - cnv: meta.vartype == "cnv" - snv: meta.vartype == "snv" - indel: meta.vartype == "indel" - other: false - } - .set{truth} + evals_ch = Channel.empty() - // branch out high confidence bed files - high_conf_ch.branch{ - def meta = it[0] - sv: meta.vartype == "sv" - small: meta.vartype == "small" - cnv: meta.vartype == "cnv" - snv: meta.vartype == "snv" - indel: meta.vartype == "indel" - other: false - } - .set{high_conf} - - // prepare benchmark sets - if(params.truth_small){ - if(params.high_conf_small){ - test.small.combine(truth.small) - .combine(high_conf.small) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi, high_meta, high_bed -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, high_bed ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - else{ - test.small.combine(truth.small) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, [] ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - } - if(params.truth_sv){ - if(params.high_conf_sv){ - test.sv.combine(truth.sv) - .combine(high_conf.sv) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi, high_meta, high_bed -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, high_bed ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - else{ - test.sv.combine(truth.sv) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, [] ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - } - if(params.truth_cnv){ - if(params.high_conf_cnv){ - test.cnv.combine(truth.cnv) - .combine(high_conf.cnv) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi, high_meta, high_bed -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, high_bed ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - else{ - test.cnv.combine(truth.cnv) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, [] ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - } - if(params.truth_snv){ - if(params.high_conf_snv){ - test.snv.combine(truth.snv) - .combine(high_conf.snv) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi, high_meta, high_bed -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, high_bed ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - else{ - test.snv.combine(truth.snv) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, [] ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - } - if(params.truth_indel){ - if(params.high_conf_indel){ - test.indel.combine(truth.indel) - .combine(high_conf.indel) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi, high_meta, high_bed -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, high_bed ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } - else{ - test.indel.combine(truth.indel) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi -> - [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, [] ] - } - .set{bench} - bench_ch = bench_ch.mix(bench) - } + if (params.variant_type == "structural"){ + // Perform SV benchmarking - for now it also works for somatic cases! + // this part will be changed! + SV_GERMLINE_BENCHMARK( + bench, + fasta, + fai + ) + ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(SV_GERMLINE_BENCHMARK.out.summary_reports) + evals_ch = evals_ch.mix(SV_GERMLINE_BENCHMARK.out.tagged_variants) } - // branch out combined benchmark sets - bench_ch.branch{ - def meta = it[0] - sv: meta.vartype == "sv" - small: meta.vartype == "small" - cnv: meta.vartype == "cnv" - snv: meta.vartype == "snv" - indel: meta.vartype == "indel" - other: false - } - .set{bench_input} - - - // Perform SV benchmarking - for now it also works for somatic cases! - SV_GERMLINE_BENCHMARK( - bench_input.sv, - fasta, - fai - ) - ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(SV_GERMLINE_BENCHMARK.out.summary_reports) - sv_evals_ch = sv_evals_ch.mix(SV_GERMLINE_BENCHMARK.out.tagged_variants) - if (params.analysis.contains("germline")){ - // Benchmarking spesific to small germline samples - SMALL_GERMLINE_BENCHMARK( - bench_input.small.mix(bench_input.snv,bench_input.indel), - fasta, - fai, - sdf - ) - ch_versions = ch_versions.mix(SMALL_GERMLINE_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(SMALL_GERMLINE_BENCHMARK.out.summary_reports) - small_evals_ch = small_evals_ch.mix(SMALL_GERMLINE_BENCHMARK.out.tagged_variants) + if (params.variant_type == "small" | params.variant_type == "snv" | params.variant_type == "indel"){ + // Benchmarking spesific to small germline samples + SMALL_GERMLINE_BENCHMARK( + bench, + fasta, + fai, + sdf + ) + ch_versions = ch_versions.mix(SMALL_GERMLINE_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(SMALL_GERMLINE_BENCHMARK.out.summary_reports) + evals_ch = evals_ch.mix(SMALL_GERMLINE_BENCHMARK.out.tagged_variants) + } - // Benchmarking spesific to CNV germline samples - CNV_GERMLINE_BENCHMARK( - bench_input.cnv, - fasta, - fai - ) - ch_versions = ch_versions.mix(CNV_GERMLINE_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(CNV_GERMLINE_BENCHMARK.out.summary_reports) + if (params.variant_type == "copynumber"){ + // Benchmarking spesific to CNV germline samples + CNV_GERMLINE_BENCHMARK( + bench, + fasta, + fai + ) + ch_versions = ch_versions.mix(CNV_GERMLINE_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(CNV_GERMLINE_BENCHMARK.out.summary_reports) + } } // TODO: SOMATIC BENCHMARKING if (params.analysis.contains("somatic")){ - somatic_small = bench_input.snv.mix(bench_input.indel) - // SOMATIC VARIANT BENCHMARKING - SMALL_SOMATIC_BENCHMARK( - somatic_small, - fasta, - fai - ) - ch_versions = ch_versions.mix(SMALL_SOMATIC_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(SMALL_SOMATIC_BENCHMARK.out.summary_reports) + if (params.variant_type == "snv" | params.variant_type == "indel"){ + // SOMATIC VARIANT BENCHMARKING + SMALL_SOMATIC_BENCHMARK( + bench, + fasta, + fai + ) + ch_versions = ch_versions.mix(SMALL_SOMATIC_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(SMALL_SOMATIC_BENCHMARK.out.summary_reports) + } } - // compare tool spesfic benchmarks COMPARE_BENCHMARK_RESULTS( - small_evals_ch, - sv_evals_ch, + evals_ch, fasta, fai ) From d080536662b48f174c09776a4c5150d34166c874 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 6 Nov 2024 15:05:39 +0000 Subject: [PATCH 191/292] remove itruth --- conf/igenomes.config | 1 + conf/test_full.config | 12 +++-- main.nf | 20 ------- nextflow.config | 22 ++++---- nextflow_schema.json | 11 +--- .../main.nf | 52 ------------------- 6 files changed, 19 insertions(+), 99 deletions(-) diff --git a/conf/igenomes.config b/conf/igenomes.config index cd55be5..188d4a2 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -37,6 +37,7 @@ params { mito_name = "chrM" macs_gsize = "2.7e9" blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" + } 'CHM13' { fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" diff --git a/conf/test_full.config b/conf/test_full.config index dd92cdd..1e44e45 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -20,17 +20,19 @@ params { max_time = '8.h' // Input data - input = null + input = 'assets/samplesheet_small.csv' outdir = 'results' // Genome references genome = 'GRCh37' analysis = 'germline' truth_id = "HG002" - - method = 'truvari,svanalyzer,happy,rtgtools' - + variant_type = "small" + method = 'happy,rtgtools' preprocess = "normalization,deduplication,filter_contigs" - min_sv_size = 30 + + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.bed" + itruth_ignore = true } diff --git a/main.nf b/main.nf index 460f496..2e63537 100644 --- a/main.nf +++ b/main.nf @@ -19,26 +19,6 @@ include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_vari params.fasta = getGenomeAttribute('fasta') params.fai = getGenomeAttribute('fai') -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - TRUTH PARAMETER VALUES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ -include { getTruthAttribute } from './subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' - -params.truth_small = getTruthAttribute('truth_small') -params.high_conf_small = getTruthAttribute('high_conf_small') -params.truth_sv = getTruthAttribute('truth_sv') -params.high_conf_sv = getTruthAttribute('high_conf_sv') -params.truth_cnv = getTruthAttribute('truth_cnv') -params.high_conf_cnv = getTruthAttribute('high_conf_cnv') -params.truth_snv = getTruthAttribute('truth_snv') -params.high_conf_snv = getTruthAttribute('high_conf_snv') -params.truth_indel = getTruthAttribute('truth_indel') -params.high_conf_indel = getTruthAttribute('high_conf_indel') -params.chain = getTruthAttribute('chain') -params.rename_chr = getTruthAttribute('rename_chr') - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS diff --git a/nextflow.config b/nextflow.config index f8484a8..c887d6a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -46,9 +46,6 @@ params { dictionary = null liftover = false - // Truth library - itruth_ignore = false - // MultiQC options multiqc_config = null multiqc_title = null @@ -209,16 +206,6 @@ podman.registry = 'quay.io' singularity.registry = 'quay.io' charliecloud.registry = 'quay.io' - - -// Load truth.config if required -if (!params.itruth_ignore) { - includeConfig 'conf/truth.config' -} else { - params.somatic = [:] - params.germline = [:] - -} // Load igenomes.config if required includeConfig !params.igenomes_ignore ? 'conf/igenomes.config' : 'conf/igenomes_ignored.config' @@ -264,6 +251,14 @@ dag { enabled = true file = "${params.outdir}/pipeline_info/pipeline_dag_${trace_timestamp}.html" } +prov { + enabled = true + formats { + bco { + file = "${params.outdir}/pipeline_info/manifest_${trace_timestamp}.bco.json" + } + } +} manifest { name = 'nf-core/variantbenchmarking' @@ -279,6 +274,7 @@ manifest { // Nextflow plugins plugins { id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-prov@1.2.2' // Provenance reports for pipeline runs } validation { diff --git a/nextflow_schema.json b/nextflow_schema.json index 8ff5b2b..c9d2a76 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -196,13 +196,6 @@ "hidden": true, "default": "s3://ngi-igenomes/igenomes/" }, - "itruth_ignore": { - "type": "boolean", - "description": "Do not load the truth reference config.", - "fa_icon": "fas fa-ban", - "hidden": true, - "help_text": "Do not load `truth.config` when running the pipeline. In this case user need to provide necessary truth files. You may choose this option if you observe clashes between custom parameters and those supplied in `truth.config`." - }, "liftover": { "type": "boolean", "description": "Run liftover workflow", @@ -216,7 +209,7 @@ "exists": true, "pattern": "^\\S+\\.(chain|bed)?(\\.gz)?$", "description": "Path to the chain file sey required for liftover.", - "help_text": "This parameter is *mandatory* if `--liftover` is true and --genome is not specified and --itruth is false. A chain can be found under itruth files and can be used together with --genome.", + "help_text": "This parameter is *mandatory* if `--liftover` is true", "fa_icon": "fas fa-file-csv" }, "rename_chr": { @@ -226,7 +219,7 @@ "mimetype": "text/plain", "pattern": "^\\S+\\.txt$", "description": "Path to the ranaming chromosomes for lifting over.", - "help_text": "This parameter is *mandatory* if `--liftover` is true and --genome is not specified and --itruth is false. A rename can be found under assets/rename_contigs or can be used together with --genome.", + "help_text": "This parameter is *mandatory* if `--liftover` is true", "fa_icon": "far fa-file-code" }, "dictionary": { diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index 2336434..4d516ea 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -154,58 +154,6 @@ def getGenomeAttribute(attribute) { return null } -// -// Get attribute from truth config file e.g. truth_small -// -def getTruthAttribute(attribute) { - if (!params.itruth_ignore){ - if (params.truth_id && params.analysis && params.genome) { - if (params.liftover){ - if (params.genome == "GRCh38"){ - if (params.analysis == "somatic"){ - if (params.somatic[ "GRCh37" ][ params.truth_id ].containsKey(attribute)){ - return params.somatic[ "GRCh37" ][ params.truth_id ][ attribute ] - } - } - else if(params.analysis == "germline") { - if (params.germline[ "GRCh37" ][ params.truth_id ].containsKey(attribute)){ - return params.germline[ "GRCh37" ][ params.truth_id ][ attribute ] - } - } - } - else if (params.genome == "GRCh37"){ - if (params.analysis == "somatic"){ - if (params.somatic[ "GRCh38" ][ params.truth_id ].containsKey(attribute)){ - return params.somatic[ "GRCh38" ][ params.truth_id ][ attribute ] - } - } - else if(params.analysis == "germline") { - if (params.germline[ "GRCh38" ][ params.truth_id ].containsKey(attribute)){ - return params.germline[ "GRCh38" ][ params.truth_id ][ attribute ] - } - } - } - } - else{ - if (params.analysis == "somatic"){ - if (params.somatic[ params.genome ][ params.truth_id ].containsKey(attribute)){ - return params.somatic[ params.genome ][ params.truth_id ][ attribute ] - } - } - else if(params.analysis == "germline") { - if (params.germline[ params.genome ][ params.truth_id ].containsKey(attribute)){ - return params.germline[ params.genome][ params.truth_id ][ attribute ] - } - } - - } - } - else{ - return null - } - } -} - // // Exit pipeline if incorrect --genome key provided // From dedd5eb13881e08e70062922a4df3002f068695f Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 6 Nov 2024 15:09:22 +0000 Subject: [PATCH 192/292] test profile for github actions --- conf/test.config | 43 +++++++++++++++++++++++++++++++++++++++++++ 1 file changed, 43 insertions(+) create mode 100644 conf/test.config diff --git a/conf/test.config b/conf/test.config new file mode 100644 index 0000000..5c34a7a --- /dev/null +++ b/conf/test.config @@ -0,0 +1,43 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Input data + input = "assets/samplesheet_small.csv" + outdir = "results" + + // Genome references + genome = 'GRCh38' + + // Processes + analysis = 'germline' + variant_type = "small" + method = 'happy,rtgtools' + preprocess = "normalization,deduplication,prepy" + include_expression = 'FILTER="."' + + // truth information + truth_id = "HG002" + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" +} From bf091c6fcd02e4a25db191fbaadb16449fa6bfd2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 6 Nov 2024 15:21:26 +0000 Subject: [PATCH 193/292] fix linting --- nextflow.config | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index c887d6a..ce30578 100644 --- a/nextflow.config +++ b/nextflow.config @@ -21,9 +21,11 @@ params { // Truth parameters truth_id = null + truth_vcf = null + regions_bed = null // Benchmarking method - method = 'truvari,svanalyzer,happy,rtgtools,wittyer' + method = 'truvari,svanalyzer,happy,rtgtools,wittyer,sompy' // Preprocess spesific parameters preprocess = "" @@ -44,6 +46,8 @@ params { igenomes_ignore = false sdf = null dictionary = null + rename_chr = null + chain = null liftover = false // MultiQC options From 748ad9b978bf64fb8dcf6e42f6e1640f1394b520 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 11 Nov 2024 14:58:04 +0000 Subject: [PATCH 194/292] add nf-test files --- .github/workflows/ci.yml | 100 +- .gitignore | 2 +- conf/modules.config | 6 +- conf/tests/germline_small.config | 43 + conf/tests/germline_sv.config | 44 + conf/tests/liftover_hg37.config | 41 + conf/tests/liftover_hg38.config | 41 + conf/tests/somatic_indel.config | 37 + conf/tests/somatic_snv.config | 36 + .../somatic_sv.config} | 26 +- modules.json | 4 +- modules/local/create_datavzrd_input/main.nf | 5 + modules/nf-core/happy/happy/environment.yml | 2 - modules/nf-core/happy/happy/main.nf | 2 +- modules/nf-core/happy/happy/meta.yml | 287 ++-- .../happy/happy/tests/main.nf.test.snap | 6 +- modules/nf-core/happy/sompy/environment.yml | 2 - modules/nf-core/happy/sompy/main.nf | 2 +- modules/nf-core/happy/sompy/meta.yml | 167 ++- .../nf-core/happy/sompy/tests/main.nf.test | 127 ++ .../happy/sompy/tests/main.nf.test.snap | 111 ++ .../nf-core/happy/sompy/tests/nextflow.config | 5 + nf-test.config | 17 + tests/.nftignore | 6 + tests/config/tags.yml | 10 + tests/germline_small.nf.test | 68 + tests/germline_small.nf.test.snap | 947 +++++++++++++ tests/germline_sv.nf.test | 69 + tests/germline_sv.nf.test.snap | 1237 +++++++++++++++++ tests/liftover_hg37.nf.test | 40 + tests/liftover_hg37.nf.test.snap | 240 ++++ tests/liftover_hg38.nf.test | 71 + tests/liftover_hg38.nf.test.snap | 971 +++++++++++++ tests/main.nf.test | 38 - tests/nextflow.config | 29 + tests/somatic_indel.nf.test | 38 + tests/somatic_indel.nf.test.snap | 185 +++ tests/somatic_snv.nf.test | 54 + tests/somatic_snv.nf.test.snap | 188 +++ tests/somatic_sv.nf.test | 38 + tests/somatic_sv.nf.test.snap | 226 +++ 41 files changed, 5307 insertions(+), 261 deletions(-) create mode 100644 conf/tests/germline_small.config create mode 100644 conf/tests/germline_sv.config create mode 100644 conf/tests/liftover_hg37.config create mode 100644 conf/tests/liftover_hg38.config create mode 100644 conf/tests/somatic_indel.config create mode 100644 conf/tests/somatic_snv.config rename conf/{test_stub.config => tests/somatic_sv.config} (59%) create mode 100644 modules/nf-core/happy/sompy/tests/main.nf.test create mode 100644 modules/nf-core/happy/sompy/tests/main.nf.test.snap create mode 100644 modules/nf-core/happy/sompy/tests/nextflow.config create mode 100644 nf-test.config create mode 100644 tests/.nftignore create mode 100644 tests/config/tags.yml create mode 100644 tests/germline_small.nf.test create mode 100644 tests/germline_small.nf.test.snap create mode 100644 tests/germline_sv.nf.test create mode 100644 tests/germline_sv.nf.test.snap create mode 100644 tests/liftover_hg37.nf.test create mode 100644 tests/liftover_hg37.nf.test.snap create mode 100644 tests/liftover_hg38.nf.test create mode 100644 tests/liftover_hg38.nf.test.snap delete mode 100644 tests/main.nf.test create mode 100644 tests/nextflow.config create mode 100644 tests/somatic_indel.nf.test create mode 100644 tests/somatic_indel.nf.test.snap create mode 100644 tests/somatic_snv.nf.test create mode 100644 tests/somatic_snv.nf.test.snap create mode 100644 tests/somatic_sv.nf.test create mode 100644 tests/somatic_sv.nf.test.snap diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 5832335..b9b76dd 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -1,16 +1,22 @@ name: nf-core CI # This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors on: - push: + pull_request: branches: - dev - pull_request: + - master release: types: [published] workflow_dispatch: env: + NFT_DIFF: "pdiff" + NFT_DIFF_ARGS: "--line-numbers --expand-tabs=2" + NFT_VER: "0.9.2" + NFT_WORKDIR: "~" NXF_ANSI_LOG: false + NXF_SINGULARITY_CACHEDIR: ${{ github.workspace }}/.singularity + NXF_SINGULARITY_LIBRARYDIR: ${{ github.workspace }}/.singularity concurrency: group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" @@ -18,42 +24,94 @@ concurrency: jobs: test: - name: Run pipeline with test data + name: "${{ matrix.NXF_VER }} | ${{ matrix.profile }} | ${{ matrix.shard }}/6" # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/variantbenchmarking') }}" + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/demultiplex') }}" runs-on: ubuntu-latest strategy: + fail-fast: false matrix: NXF_VER: - "24.04.2" - - "latest-everything" + - "latest-stable" + profile: + - "docker" + - "singularity" + isMaster: + - ${{ github.base_ref == 'master' }} + # Exclude conda and singularity on dev + exclude: + - isMaster: false + profile: "conda" + - isMaster: false + profile: "singularity" + shard: [1, 2, 3, 4, 5, 6] steps: - name: Check out pipeline code uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + with: + fetch-depth: 0 - - name: Install Nextflow + - name: Set up Nextflow uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" - - name: Disk space cleanup - uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Set up Apptainer + if: matrix.profile == 'singularity' + uses: eWaterCycle/setup-apptainer@main + + - name: Set up Singularity + if: matrix.profile == 'singularity' + run: | + mkdir -p $NXF_SINGULARITY_CACHEDIR + mkdir -p $NXF_SINGULARITY_LIBRARYDIR + + - name: Set up Miniconda + if: matrix.profile == 'conda' + uses: conda-incubator/setup-miniconda@a4260408e20b96e80095f42ff7f1a15b27dd94ca # v3 + with: + miniconda-version: "latest" + auto-update-conda: true + conda-solver: libmamba + channels: conda-forge,bioconda - - name: Run pipeline with test data for germline analysis (docker) - # TODO nf-core: You can customise CI pipeline run tests as required - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix + - name: Set up Conda + if: matrix.profile == 'conda' run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,docker --outdir ./results + echo $(realpath $CONDA)/condabin >> $GITHUB_PATH + echo $(realpath python) >> $GITHUB_PATH - - name: Run pipeline with test data (singularity) - # TODO nf-core: You can customise CI pipeline run tests as required + - name: Clean up Disk space + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@v4 + with: + python-version: "3.11" + architecture: "x64" + + - name: Install pdiff to see diff between nf-test snapshots run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,singularity --outdir ./results - if: "${{ github.base_ref == 'master' }}" + python -m pip install --upgrade pip + pip install pdiff - - name: Run pipeline with test data (conda) - # TODO nf-core: You can customise CI pipeline run tests as required + - uses: nf-core/setup-nf-test@v1 + with: + version: ${{ env.NFT_VER }} + + - name: Run Tests (Shard ${{ matrix.shard }}/6) run: | - nextflow run ${GITHUB_WORKSPACE} -profile test,conda --outdir ./results - if: "${{ github.base_ref == 'master' }}" + nf-test test \ + --ci \ + --shard ${{ matrix.shard }}/6 \ + --changed-since HEAD^ \ + --profile "+${{ matrix.profile }}" \ + --filter pipeline \ + --junitxml=test.xml + + - name: Publish Test Report + uses: mikepenz/action-junit-report@v3 + if: always() # always run even if the previous step fails + with: + report_paths: test.xml + annotate_only: true diff --git a/.gitignore b/.gitignore index 57b3201..a0fbbc4 100644 --- a/.gitignore +++ b/.gitignore @@ -13,4 +13,4 @@ null/* results*/* .nf-test/ .nf-test.log - +test/.nf-test.log diff --git a/conf/modules.config b/conf/modules.config index d5ff443..9210b89 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,7 +39,7 @@ process { ] } withName: SVYNC { - ext.prefix = {"${meta.id}.${meta.caller}"} + ext.prefix = {"${meta.id}.${meta.caller}.svync"} publishDir = [ path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz,vcf.gz.tbi}", @@ -192,7 +192,7 @@ process { ] } withName: "HAPPY_PREPY" { - ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} + ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}.prepy"} ext.args = {"--fixchr --filter-nonref --bcftools-norm"} publishDir = [ path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, @@ -315,7 +315,7 @@ process { ] } withName: PICARD_LIFTOVERVCF { - ext.prefix = {"${meta.id}}"} + ext.prefix = {"${meta.id}"} ext.args = {"--WARN_ON_MISSING_CONTIG true"} } withName: BCFTOOLS_RENAME_CHR { diff --git a/conf/tests/germline_small.config b/conf/tests/germline_small.config new file mode 100644 index 0000000..5c09764 --- /dev/null +++ b/conf/tests/germline_small.config @@ -0,0 +1,43 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Input data + input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_small.csv" + outdir = "results" + + // Genome references + genome = 'GRCh38' + + // Processes + analysis = 'germline' + variant_type = "small" + method = 'happy,rtgtools' + preprocess = "normalization,deduplication,prepy" + include_expression = 'FILTER="."' + + // truth information + truth_id = "HG002" + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" +} diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config new file mode 100644 index 0000000..615f5c4 --- /dev/null +++ b/conf/tests/germline_sv.config @@ -0,0 +1,44 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Input data + input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_sv.csv" + outdir = "results" + + // Genome references + genome = 'GRCh38' + + // Processes + analysis = 'germline' + variant_type = "structural" + method = 'svanalyzer,wittyer,truvari' + preprocess = "normalization,deduplication" + sv_standardization = "svync,homogenize" + min_sv_size = 30 + truth_id = "HG002" + include_expression = 'FILTER="."' + + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" +} diff --git a/conf/tests/liftover_hg37.config b/conf/tests/liftover_hg37.config new file mode 100644 index 0000000..ae4957e --- /dev/null +++ b/conf/tests/liftover_hg37.config @@ -0,0 +1,41 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/benchmark -profile test_liftover, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.5GB' + max_time = '8.h' + + // Input data + input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_sv_hg37.csv' + outdir = 'results' + genome = 'GRCh37' + + // Processes + analysis = 'germline' + variant_type = "structural" + method = 'truvari' + preprocess = "normalization,deduplication,filter_contigs" + min_sv_size = 30 + + truth_id = "HG002" + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + + //liftover files + liftover = true + chain = "http://ftp.ensembl.org/pub/assembly_mapping/homo_sapiens/GRCh38_to_GRCh37.chain.gz" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch38_grch37.txt" +} diff --git a/conf/tests/liftover_hg38.config b/conf/tests/liftover_hg38.config new file mode 100644 index 0000000..25a0e58 --- /dev/null +++ b/conf/tests/liftover_hg38.config @@ -0,0 +1,41 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/benchmark -profile test_liftover, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.5GB' + max_time = '8.h' + + // Input data + input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_small.csv' + outdir = 'results' + genome = 'GRCh38' + + // Processes + analysis = 'germline' + truth_id = "HG002" + variant_type = "small" + method = 'rtgtools,happy' + preprocess = "normalization,deduplication,filter_contigs" + + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" + + //liftover files + liftover = true + chain = "https://raw.githubusercontent.com/broadinstitute/gatk/master/scripts/funcotator/data_sources/gnomAD/b37ToHg38.over.chain" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" +} diff --git a/conf/tests/somatic_indel.config b/conf/tests/somatic_indel.config new file mode 100644 index 0000000..fbd1dc2 --- /dev/null +++ b/conf/tests/somatic_indel.config @@ -0,0 +1,37 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.5GB' + max_time = '8.h' + + // Input data + input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_somatic_indel.csv' + outdir = 'results' + + // Genome references + genome = 'GRCh38' + analysis = 'somatic' + truth_id = "SEQC2" + method = 'sompy' + preprocess = "filter_contigs" + variant_type = "indel" + + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sINDEL_truth_set_v1.0.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" + +} diff --git a/conf/tests/somatic_snv.config b/conf/tests/somatic_snv.config new file mode 100644 index 0000000..09f3884 --- /dev/null +++ b/conf/tests/somatic_snv.config @@ -0,0 +1,36 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.5GB' + max_time = '8.h' + + // Input data + input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_somatic_snv.csv' + outdir = 'results' + + // Genome references + genome = 'GRCh38' + analysis = 'somatic' + method = 'sompy' + preprocess = "filter_contigs" + + variant_type = "snv" + truth_id = "SEQC2" + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sSNV_truth_set_v1.0.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" +} diff --git a/conf/test_stub.config b/conf/tests/somatic_sv.config similarity index 59% rename from conf/test_stub.config rename to conf/tests/somatic_sv.config index 8ecbb74..5d75ac5 100644 --- a/conf/test_stub.config +++ b/conf/tests/somatic_sv.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_stub -stub --outdir + nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir ---------------------------------------------------------------------------------------- */ @@ -20,20 +20,16 @@ params { max_time = '8.h' // Input data - sample = "HG002" - input = "assets/samplesheet_sv.csv" - outdir = "results" + input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_somatic_sv.csv' + outdir = 'results' // Genome references - genome = 'GRCh38' - - // Processes - analysis = 'germline' - method = 'happy,truvari,svanalyzer,wittyer,rtgtools' // - preprocess = "normalization,deduplication,prepy" - sv_standardization = "svync,homogenize" - include_expression = 'FILTER="."' - min_sv_size = 30 - liftover = true - + genome = 'GRCh38' + analysis = 'somatic' + variant_type = "structural" + method = 'truvari' + preprocess = "filter_contigs" + + truth_id = "SEQC2" + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/13059_2022_2816_MOESM4_ESM.vcf.gz" } diff --git a/modules.json b/modules.json index c8ebf59..c7c642f 100644 --- a/modules.json +++ b/modules.json @@ -62,7 +62,7 @@ }, "happy/happy": { "branch": "master", - "git_sha": "41fc46dfd94dddf4fdee633629090c1c3bc9f668", + "git_sha": "2f04da318223fd982f8aa3d5546dabdf42afa4eb", "installed_by": ["modules"] }, "happy/prepy": { @@ -72,7 +72,7 @@ }, "happy/sompy": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "2f04da318223fd982f8aa3d5546dabdf42afa4eb", "installed_by": ["modules"] }, "manta/convertinversion": { diff --git a/modules/local/create_datavzrd_input/main.nf b/modules/local/create_datavzrd_input/main.nf index d19edea..1042dc4 100644 --- a/modules/local/create_datavzrd_input/main.nf +++ b/modules/local/create_datavzrd_input/main.nf @@ -14,4 +14,9 @@ process CREATE_DATAVZRD_INPUT { cat "$template" | sed "s|CSVPATH|$csv|g" > config.yaml """ + + stub: + """ + touch config.yaml + """ } diff --git a/modules/nf-core/happy/happy/environment.yml b/modules/nf-core/happy/happy/environment.yml index 3d29dcb..d65fa5d 100644 --- a/modules/nf-core/happy/happy/environment.yml +++ b/modules/nf-core/happy/happy/environment.yml @@ -1,7 +1,5 @@ -name: happy_happy channels: - conda-forge - bioconda - - defaults dependencies: - bioconda::hap.py=0.3.14 diff --git a/modules/nf-core/happy/happy/main.nf b/modules/nf-core/happy/happy/main.nf index a5757db..a883ce2 100644 --- a/modules/nf-core/happy/happy/main.nf +++ b/modules/nf-core/happy/happy/main.nf @@ -36,7 +36,7 @@ process HAPPY_HAPPY { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def regions = regions_bed ? "-R ${regions_bed}" : "" + def regions = regions_bed ? "-f ${regions_bed}" : "" def targets = targets_bed ? "-T ${targets_bed}" : "" def false_positives = false_positives_bed ? "--false-positives ${false_positives_bed}" : "" def stratification = stratification_tsv ? "--stratification ${stratification_tsv}" : "" diff --git a/modules/nf-core/happy/happy/meta.yml b/modules/nf-core/happy/happy/meta.yml index dc8ebf2..3582ade 100644 --- a/modules/nf-core/happy/happy/meta.yml +++ b/modules/nf-core/happy/happy/meta.yml @@ -1,5 +1,8 @@ name: "happy_happy" -description: Hap.py is a tool to compare diploid genotypes at haplotype level. Rather than comparing VCF records row by row, hap.py will generate and match alternate sequences in a superlocus. A superlocus is a small region of the genome (sized between 1 and around 1000 bp) that contains one or more variants. +description: Hap.py is a tool to compare diploid genotypes at haplotype level. Rather + than comparing VCF records row by row, hap.py will generate and match alternate + sequences in a superlocus. A superlocus is a small region of the genome (sized between + 1 and around 1000 bp) that contains one or more variants. keywords: - happy - benchmark @@ -12,127 +15,193 @@ tools: documentation: "https://github.com/Illumina/hap.py" tool_dev_url: "https://github.com/Illumina/hap.py" licence: ["BSD-2-clause"] + identifier: biotools:happy input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - meta2: - type: map - description: | - Groovy Map containing fasta file information - e.g. [ id:'test2'] - - meta3: - type: map - description: | - Groovy Map containing fai file information - e.g. [ id:'test3'] - - meta4: - type: map - description: | - Groovy Map containing false_positives_bed file information - e.g. [ id:'test4'] - - meta5: - type: map - description: | - Groovy Map containing stratification_tsv file information - e.g. [ id:'test5'] - - meta6: - type: map - description: | - Groovy Map containing stratification_beds file information - e.g. [ id:'test6'] - - query_vcf: - type: file - description: VCF/GVCF file to query - pattern: "*.{gvcf,vcf}.gz" - - truth_vcf: - type: file - description: gold standard VCF file - pattern: "*.{gvcf,vcf}.gz" - - regions_bed: - type: file - description: Sparse regions to restrict the analysis to - pattern: "*.bed" - - targets_bed: - type: file - description: Dense regions to restrict the analysis to - pattern: "*.bed" - - fasta: - type: file - description: FASTA file of the reference genome - pattern: "*.{fa,fasta}" - - fasta_fai: - type: file - description: The index of the reference FASTA - pattern: "*.fai" - - false_positives_bed: - type: file - description: False positive / confident call regions. Calls outside these regions will be labelled as UNK. - pattern: "*.{bed,bed.gz}" - - stratification_tsv: - type: file - description: Stratification file list in TSV format - pattern: "*.tsv" - - stratification_beds: - type: file - description: One or more BED files used for stratification (these should be referenced in the stratification TSV) - pattern: "*.bed" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - query_vcf: + type: file + description: VCF/GVCF file to query + pattern: "*.{gvcf,vcf}.gz" + - truth_vcf: + type: file + description: gold standard VCF file + pattern: "*.{gvcf,vcf}.gz" + - regions_bed: + type: file + description: Sparse regions to restrict the analysis to + pattern: "*.bed" + - targets_bed: + type: file + description: Dense regions to restrict the analysis to + pattern: "*.bed" + - - meta2: + type: map + description: | + Groovy Map containing fasta file information + e.g. [ id:'test2'] + - fasta: + type: file + description: FASTA file of the reference genome + pattern: "*.{fa,fasta}" + - - meta3: + type: map + description: | + Groovy Map containing fai file information + e.g. [ id:'test3'] + - fasta_fai: + type: file + description: The index of the reference FASTA + pattern: "*.fai" + - - meta4: + type: map + description: | + Groovy Map containing false_positives_bed file information + e.g. [ id:'test4'] + - false_positives_bed: + type: file + description: False positive / confident call regions. Calls outside these regions + will be labelled as UNK. + pattern: "*.{bed,bed.gz}" + - - meta5: + type: map + description: | + Groovy Map containing stratification_tsv file information + e.g. [ id:'test5'] + - stratification_tsv: + type: file + description: Stratification file list in TSV format + pattern: "*.tsv" + - - meta6: + type: map + description: | + Groovy Map containing stratification_beds file information + e.g. [ id:'test6'] + - stratification_beds: + type: file + description: One or more BED files used for stratification (these should be + referenced in the stratification TSV) + pattern: "*.bed" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - summary_csv: - type: file - description: A CSV file containing the summary of the benchmarking - pattern: "*.summary.csv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.summary.csv": + type: file + description: A CSV file containing the summary of the benchmarking + pattern: "*.summary.csv" - roc_all_csv: - type: file - description: A CSV file containing ROC values for all variants - pattern: "*.roc.all.csv.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.roc.all.csv.gz": + type: file + description: A CSV file containing ROC values for all variants + pattern: "*.roc.all.csv.gz" - roc_indel_locations_csv: - type: file - description: A CSV file containing ROC values for all indels - pattern: "*.roc.Locations.INDEL.csv.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.roc.Locations.INDEL.csv.gz": + type: file + description: A CSV file containing ROC values for all indels + pattern: "*.roc.Locations.INDEL.csv.gz" - roc_indel_locations_pass_csv: - type: file - description: A CSV file containing ROC values for all indels that passed all filters - pattern: "*.roc.Locations.INDEL.PASS.csv.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.roc.Locations.INDEL.PASS.csv.gz": + type: file + description: A CSV file containing ROC values for all indels that passed all + filters + pattern: "*.roc.Locations.INDEL.PASS.csv.gz" - roc_snp_locations_csv: - type: file - description: A CSV file containing ROC values for all SNPs - pattern: "*.roc.Locations.SNP.csv.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.roc.Locations.SNP.csv.gz": + type: file + description: A CSV file containing ROC values for all SNPs + pattern: "*.roc.Locations.SNP.csv.gz" - roc_snp_locations_pass_csv: - type: file - description: A CSV file containing ROC values for all SNPs that passed all filters - pattern: "*.roc.Locations.SNP.PASS.csv.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.roc.Locations.SNP.PASS.csv.gz": + type: file + description: A CSV file containing ROC values for all SNPs that passed all filters + pattern: "*.roc.Locations.SNP.PASS.csv.gz" - extended_csv: - type: file - description: A CSV file containing extended info of the benchmarking - pattern: "*.extended.csv" - - metrics_json: - type: file - description: A JSON file containing the run info - pattern: "*.runinfo.json" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.extended.csv": + type: file + description: A CSV file containing extended info of the benchmarking + pattern: "*.extended.csv" - runinfo: - type: file - description: A JSON file containing the benchmarking metrics - pattern: "*.metrics.json.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.runinfo.json": + type: file + description: A JSON file containing the benchmarking metrics + pattern: "*.metrics.json.gz" + - metrics_json: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.metrics.json.gz": + type: file + description: A JSON file containing the run info + pattern: "*.runinfo.json" - vcf: - type: file - description: An annotated VCF - pattern: "*.vcf.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.vcf.gz": + type: file + description: An annotated VCF + pattern: "*.vcf.gz" - tbi: - type: file - description: The index of the annotated VCF - pattern: "*.tbi" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.tbi": + type: file + description: The index of the annotated VCF + pattern: "*.tbi" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@nvnieuwk" maintainers: diff --git a/modules/nf-core/happy/happy/tests/main.nf.test.snap b/modules/nf-core/happy/happy/tests/main.nf.test.snap index 5c69e7f..75516db 100644 --- a/modules/nf-core/happy/happy/tests/main.nf.test.snap +++ b/modules/nf-core/happy/happy/tests/main.nf.test.snap @@ -24,7 +24,7 @@ { "id": "test" }, - "test.extended.csv:md5,4b53c835cd20fe0b14fcb4b2ef6b0f52" + "test.extended.csv:md5,5ec5e0b3a260ddcb53a2628871547d79" ] ], [ @@ -32,10 +32,10 @@ ] ], "meta": { - "nf-test": "0.9.0", + "nf-test": "0.9.1", "nextflow": "24.04.4" }, - "timestamp": "2024-08-19T14:11:48.021071904" + "timestamp": "2024-10-28T10:29:30.466331046" }, "versions_1": { "content": [ diff --git a/modules/nf-core/happy/sompy/environment.yml b/modules/nf-core/happy/sompy/environment.yml index 64ed7f5..d65fa5d 100644 --- a/modules/nf-core/happy/sompy/environment.yml +++ b/modules/nf-core/happy/sompy/environment.yml @@ -1,7 +1,5 @@ -name: happy_sompy channels: - conda-forge - bioconda - - defaults dependencies: - bioconda::hap.py=0.3.14 diff --git a/modules/nf-core/happy/sompy/main.nf b/modules/nf-core/happy/sompy/main.nf index e0c34e2..ba23fd1 100644 --- a/modules/nf-core/happy/sompy/main.nf +++ b/modules/nf-core/happy/sompy/main.nf @@ -54,7 +54,7 @@ process HAPPY_SOMPY { """ stub: - def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" def VERSION = '0.3.14' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ touch ${prefix}.features.csv diff --git a/modules/nf-core/happy/sompy/meta.yml b/modules/nf-core/happy/sompy/meta.yml index 3dd2b91..f7847c4 100644 --- a/modules/nf-core/happy/sompy/meta.yml +++ b/modules/nf-core/happy/sompy/meta.yml @@ -1,5 +1,6 @@ name: "happy_sompy" -description: Hap.py is a tool to compare diploid genotypes at haplotype level. som.py is a part of hap.py compares somatic variations. +description: Hap.py is a tool to compare diploid genotypes at haplotype level. som.py + is a part of hap.py compares somatic variations. keywords: - happy - sompy @@ -13,74 +14,114 @@ tools: homepage: "https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub/apps/hap-py-benchmarking.html" documentation: "https://github.com/Illumina/hap.py/blob/master/doc/sompy.md" tool_dev_url: "https://github.com/Illumina/hap.py" - - licence: "['BSD-2-clause']" + licence: ["BSD-2-clause"] + identifier: biotools:happy input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - query_vcf: - type: file - description: VCF/GVCF file to query - pattern: "*.{gvcf,vcf}.gz" - - truth_vcf: - type: file - description: gold standard VCF file - pattern: "*.{gvcf,vcf}.gz" - - regions_bed: - type: file - description: Sparse regions to restrict the analysis to - pattern: "*.bed" - - targets_bed: - type: file - description: Dense regions to restrict the analysis to - pattern: "*.bed" - - fasta: - type: file - description: FASTA file of the reference genome - pattern: "*.{fa,fasta}" - - fasta_fai: - type: file - description: The index of the reference FASTA - pattern: "*.fai" - - false_positives_bed: - type: file - description: False positive / confident call regions. Calls outside these regions will be labelled as UNK. - pattern: "*.{bed,bed.gz}" - - stratification_tsv: - type: file - description: Ambiguous region bed file(s) - pattern: "*.bed" - - bams: - type: file - description: one or more BAM files for feature table extraction - pattern: "*.bam" - + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - query_vcf: + type: file + description: VCF/GVCF file to query + pattern: "*.{gvcf,vcf}.gz" + - truth_vcf: + type: file + description: gold standard VCF file + pattern: "*.{gvcf,vcf}.gz" + - regions_bed: + type: file + description: Sparse regions to restrict the analysis to + pattern: "*.bed" + - targets_bed: + type: file + description: Dense regions to restrict the analysis to + pattern: "*.bed" + - - meta2: + type: map + description: | + Groovy Map containing fasta file information + e.g. [ id:'test2'] + - fasta: + type: file + description: FASTA file of the reference genome + pattern: "*.{fa,fasta}" + - - meta3: + type: map + description: | + Groovy Map containing fai file information + e.g. [ id:'test3'] + - fasta_fai: + type: file + description: The index of the reference FASTA + pattern: "*.fai" + - - meta4: + type: map + description: | + Groovy Map containing false_positives_bed file information + e.g. [ id:'test4'] + - false_positives_bed: + type: file + description: False positive / confident call regions. Calls outside these regions + will be labelled as UNK. + pattern: "*.{bed,bed.gz}" + - - meta5: + type: map + description: | + Groovy Map containing ambiguous_beds file information + e.g. [ id:'test5'] + - ambiguous_beds: + type: file + description: Ambiguous regions + pattern: "*.{bed,bed.gz}" + - - meta6: + type: map + description: | + Groovy Map containing bam file information + e.g. [ id:'test6'] + - bams: + type: file + description: one or more BAM files for feature table extraction + pattern: "*.bam" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - features: - type: file - description: One or more than one (if AF count is on ) CSV file containing feature information - pattern: "*.features.csv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.features.csv": + type: file + description: One or more than one (if AF count is on ) CSV file containing feature + information + pattern: "*.features.csv" - metrics: - type: file - description: One or more than one (if AF count is on ) JSON file with metrics - pattern: "*.metrics.json" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.metrics.json": + type: file + description: One or more than one (if AF count is on ) JSON file with metrics + pattern: "*.metrics.json" - stats: - type: file - description: One or more than one (if AF count is on ) CSV file with benchmark stats - pattern: "*.stats.csv" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.stats.csv": + type: file + description: One or more than one (if AF count is on ) CSV file with benchmark + stats + pattern: "*.stats.csv" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@kubranarci" diff --git a/modules/nf-core/happy/sompy/tests/main.nf.test b/modules/nf-core/happy/sompy/tests/main.nf.test new file mode 100644 index 0000000..f4919c7 --- /dev/null +++ b/modules/nf-core/happy/sompy/tests/main.nf.test @@ -0,0 +1,127 @@ +nextflow_process { + + name "Test Process HAPPY_SOMPY" + script "../main.nf" + process "HAPPY_SOMPY" + + tag "modules" + tag "modules_nfcore" + tag "happy" + tag "happy/sompy" + + test("homo_sapiens - vcf") { + config './nextflow.config' + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true) + ] + input[3] = [[],[]] + input[4] = [[],[]] + input[5] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.stats, + process.out.versions, + file(process.out.metrics[0][1]).name, + file(process.out.features[0][1]).name).match()} + ) + } + + } + test("homo_sapiens - vcf - bam") { + config './nextflow.config' + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true) + ] + input[3] = [[],[]] + input[4] = [[],[]] + input[5] = [[ id:'test2' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.sorted.bam', checkIfExists: true)] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.stats, + process.out.versions, + file(process.out.metrics[0][1]).name, + file(process.out.features[0][1]).name).match()} + ) + } + + } + test("homo_sapiens - vcf - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/paired_mutect2_calls/test_test2_paired_mutect2_calls.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/gatk/haplotypecaller_calls/test2_haplotc.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/multi_intervals.bed', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'test2' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta', checkIfExists: true) + ] + input[2] = [ + [ id:'test3' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai', checkIfExists: true) + ] + input[3] = [[],[]] + input[4] = [[],[]] + input[5] = [[],[]] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/happy/sompy/tests/main.nf.test.snap b/modules/nf-core/happy/sompy/tests/main.nf.test.snap new file mode 100644 index 0000000..e483f1c --- /dev/null +++ b/modules/nf-core/happy/sompy/tests/main.nf.test.snap @@ -0,0 +1,111 @@ +{ + "homo_sapiens - vcf": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.stats.csv:md5,0fa86a9726fcc3e2172342dd1305cf82" + ] + ], + [ + "versions.yml:md5,dda929752e25faa5e5dc8e82228980fc" + ], + "test.metrics.json", + "test.features.csv" + ], + "meta": { + "nf-test": "0.9.1", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-28T10:43:13.082233148" + }, + "homo_sapiens - vcf - bam": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.stats.csv:md5,2502fe315dcf32d00c9961b9462759aa" + ] + ], + [ + "versions.yml:md5,dda929752e25faa5e5dc8e82228980fc" + ], + "test.metrics.json", + "test.features.csv" + ], + "meta": { + "nf-test": "0.9.1", + "nextflow": "24.04.4" + }, + "timestamp": "2024-10-28T10:43:32.122793995" + }, + "homo_sapiens - vcf - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.metrics.json:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test" + }, + "test.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,dda929752e25faa5e5dc8e82228980fc" + ], + "features": [ + [ + { + "id": "test" + }, + "test.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "metrics": [ + [ + { + "id": "test" + }, + "test.metrics.json:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "stats": [ + [ + { + "id": "test" + }, + "test.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,dda929752e25faa5e5dc8e82228980fc" + ] + } + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.04.4" + }, + "timestamp": "2024-08-23T14:39:09.160343644" + } +} \ No newline at end of file diff --git a/modules/nf-core/happy/sompy/tests/nextflow.config b/modules/nf-core/happy/sompy/tests/nextflow.config new file mode 100644 index 0000000..3a95dc4 --- /dev/null +++ b/modules/nf-core/happy/sompy/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: HAPPY_SOMPY { + ext.args = '--feature-table hcc.mutect.snv' + } +} diff --git a/nf-test.config b/nf-test.config new file mode 100644 index 0000000..0bf7ea8 --- /dev/null +++ b/nf-test.config @@ -0,0 +1,17 @@ +config { + // Location of nf-tests + testsDir "tests" + + // nf-test directory used to create temporary files for each test + workDir System.getenv("NFT_WORKDIR") ?: ".nf-test" + + // Location of an optional nextflow.config file specific for executing pipeline tests + configFile "tests/nextflow.config" + + profile "docker,test" + + // load the necessary plugins ?? + plugins { + load "nft-utils@0.0.3" + } +} diff --git a/tests/.nftignore b/tests/.nftignore new file mode 100644 index 0000000..02ba6e4 --- /dev/null +++ b/tests/.nftignore @@ -0,0 +1,6 @@ +**/*_report.html +**/multiqc/multiqc_data/* +**/multiqc/multiqc_plots/{svg,pdf,png}/*.{svg,pdf,png} +**/multiqc/multiqc_report.html +pipeline_info/*.{html,json,txt,yml} +**/summary/tables/* diff --git a/tests/config/tags.yml b/tests/config/tags.yml new file mode 100644 index 0000000..dce2348 --- /dev/null +++ b/tests/config/tags.yml @@ -0,0 +1,10 @@ +default: + - bin/** + - conf/** + - lib/** + - modules/** + - subworkflows/** + - tests/** + - workflows/** + - nextflow.config + - main.nf diff --git a/tests/germline_small.nf.test b/tests/germline_small.nf.test new file mode 100644 index 0000000..ace53fa --- /dev/null +++ b/tests/germline_small.nf.test @@ -0,0 +1,68 @@ +nextflow_pipeline { + + name "Test pipeline for small germline variants, tested benchmarking methods are happy and rtgtools" + script "../main.nf" + tag "pipeline" + tag "germline" + tag "small" + config "../conf/tests/germline_small.config" + + test("Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } + test("-stub") { + + options "-stub" + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap new file mode 100644 index 0000000..2171e66 --- /dev/null +++ b/tests/germline_small.nf.test.snap @@ -0,0 +1,947 @@ +{ + "-stub": { + "content": [ + 76, + { + "BCFTOOLS_DEDUP": { + "bcftools": 1.18 + }, + "BCFTOOLS_MERGE": { + "bcftools": 1.2 + }, + "BCFTOOLS_NORM": { + "bcftools": 1.18 + }, + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_SORT": { + "bcftools": 1.18 + }, + "BCFTOOLS_STATS": { + "bcftools": 1.18 + }, + "DATAVZRD": { + "datavzrd": "2.36.12" + }, + "HAPPY_HAPPY": { + "hap.py": "0.3.14" + }, + "HAPPY_PREPY": { + "pre.py": "0.3.14" + }, + "MERGE_REPORTS": { + "python": "3.8.6" + 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germline variants, tested benchmarking methods are truvari, svbenchmark and wittyer" + script "../main.nf" + tag "pipeline" + tag "germline" + tag "structural" + config "../conf/tests/germline_sv.config" + + test("Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } + test("-stub") { + + options "-stub" + + when { + params { + outdir = "$outputDir" + + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap new file mode 100644 index 0000000..b7d5d4e --- /dev/null +++ b/tests/germline_sv.nf.test.snap @@ -0,0 +1,1237 @@ +{ + "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'": { + "content": [ + 138, + { + "BCFTOOLS_DEDUP": { + "bcftools": 1.18 + }, + "BCFTOOLS_NORM": { + "bcftools": 1.18 + }, + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_SORT": { + "bcftools": 1.18 + }, + "BCFTOOLS_STATS": { + "bcftools": 1.18 + }, + "BGZIP_TABIX": { + "tabix": 1.12 + }, + "DATAVZRD": { + "datavzrd": "2.36.12" + }, + "MERGE_REPORTS": { + "python": "3.8.6" + }, + "PLOTS": { + "r-base": "4.3.1" + }, + "SURVIVOR_MERGE": { + 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--git a/tests/liftover_hg37.nf.test b/tests/liftover_hg37.nf.test new file mode 100644 index 0000000..f68fc24 --- /dev/null +++ b/tests/liftover_hg37.nf.test @@ -0,0 +1,40 @@ +nextflow_pipeline { + + name "Test pipeline for small germline variants, tested benchmarking methods are truvari" + script "../main.nf" + tag "pipeline" + tag "structural" + tag "germline" + tag "liftover" + tag "hg37" + config "../conf/tests/liftover_hg37.config" + + test("Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/liftover_hg37.nf.test.snap b/tests/liftover_hg37.nf.test.snap new file mode 100644 index 0000000..e8ebe30 --- /dev/null +++ b/tests/liftover_hg37.nf.test.snap @@ -0,0 +1,240 @@ +{ + "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover": { + "content": [ + 82, + { + "BCFTOOLS_DEDUP": { + "bcftools": 1.18 + }, + "BCFTOOLS_NORM": { + "bcftools": 1.18 + }, + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_SORT": { + "bcftools": 1.18 + }, + 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"structural/HG002", + "structural/HG002/preprocess", + "structural/HG002/preprocess/HG002.renamechr.rh.norm.dedup.sort.vcf.gz", + "structural/HG002/stats", + "structural/HG002/stats/bcftools", + "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", + "structural/HG002/stats/survivor", + "structural/HG002/stats/survivor/HG002.stats", + "structural/multiqc", + "structural/multiqc/multiqc_data", + "structural/multiqc/multiqc_data/multiqc.log", + "structural/multiqc/multiqc_data/multiqc_citations.txt", + "structural/multiqc/multiqc_data/multiqc_data.json", + "structural/multiqc/multiqc_data/multiqc_software_versions.txt", + "structural/multiqc/multiqc_data/multiqc_sources.txt", + "structural/multiqc/multiqc_report.html", + "structural/summary", + "structural/summary/comparisons", + "structural/summary/comparisons/structural", + "structural/summary/comparisons/structural/truvari.FN.csv", + "structural/summary/comparisons/structural/truvari.FP.csv", + 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'small' --method 'happy,rtgtools' --liftover") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } + test("-stub") { + + options "-stub" + + when { + params { + outdir = "$outputDir" + + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap new file mode 100644 index 0000000..c227c35 --- /dev/null +++ b/tests/liftover_hg38.nf.test.snap @@ -0,0 +1,971 @@ +{ + "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover": { + "content": [ + 85, + { + "BCFTOOLS_DEDUP": { + "bcftools": 1.18 + }, + "BCFTOOLS_MERGE": { + "bcftools": 1.2 + }, + "BCFTOOLS_NORM": { + "bcftools": 1.18 + }, + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_SORT": { + "bcftools": 1.18 + }, + "BCFTOOLS_STATS": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_CONTIGS": { + "bcftools": 1.18 + }, + "DATAVZRD": { + "datavzrd": "2.36.12" + }, + "HAPPY_HAPPY": { + "hap.py": "0.3.14" + }, + "MERGE_REPORTS": { + "python": "3.8.6" + }, + "PLOTS": { + "r-base": "4.3.1" + }, + "REFORMAT_HEADER": { + "tabix": 1.12 + }, + "RTGTOOLS_FORMAT": { + "rtg-tools": "3.12.1" + }, + "RTGTOOLS_VCFEVAL": { + "rtg-tools": "3.12.1" + }, + "TABIX_BGZIP": { + "tabix": "1.19.1" 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"normalization,deduplication,prepy" - sv_standardization = "svync,homogenize" - include_expression = 'FILTER="."' - min_sv_size = 30 - truth_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - high_conf_small = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" - truth_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - high_conf_sv = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" - itruth_ignore = true - } - } - - then { - assertAll( - { assert workflow.success } - ) - } - } -} diff --git a/tests/nextflow.config b/tests/nextflow.config new file mode 100644 index 0000000..5b561b5 --- /dev/null +++ b/tests/nextflow.config @@ -0,0 +1,29 @@ +/* +======================================================================================== + Nextflow config file for running tests +======================================================================================== +*/ + +params { + // Base directory for nf-core/modules test data + modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/' + + // for hisat2 + + validationSchemaIgnoreParams = 'genomes,igenomes_base,modules_testdata_base_path,pipelines_testdata_base_path' + + // TODO: check if we rather do this or disable publishdir for all processes when testing modules/subworkflows + outdir = 'results' +} + + +// Impose same minimum Nextflow version as the pipeline for testing +manifest { + nextflowVersion = '!>=23.04.0' +} + +// Disable all Nextflow reporting options +timeline { enabled = false } +report { enabled = false } +trace { enabled = false } +dag { enabled = false } diff --git a/tests/somatic_indel.nf.test b/tests/somatic_indel.nf.test new file mode 100644 index 0000000..bba5bae --- /dev/null +++ b/tests/somatic_indel.nf.test @@ -0,0 +1,38 @@ +nextflow_pipeline { + + name "Test pipeline for small somatic variants, tested benchmarking methods are sompy" + script "../main.nf" + tag "pipeline" + tag "somatic" + tag "indel" + config "../conf/tests/somatic_indel.config" + + test("Params: --analysis 'somatic' --variant_type 'indel' --method 'sompy'") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap new file mode 100644 index 0000000..cad96d0 --- /dev/null +++ b/tests/somatic_indel.nf.test.snap @@ -0,0 +1,185 @@ +{ + "Params: --analysis 'somatic' --variant_type 'indel' --method 'sompy'": { + "content": [ + 27, + { + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_SORT": { + "bcftools": 1.18 + }, + "BCFTOOLS_STATS": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_CONTIGS": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_FILTERMISSING": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_SUBSAMPLE": { + 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"$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap new file mode 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"2024-11-11T14:36:57.021757547" + } +} \ No newline at end of file diff --git a/tests/somatic_sv.nf.test b/tests/somatic_sv.nf.test new file mode 100644 index 0000000..3d2cc3b --- /dev/null +++ b/tests/somatic_sv.nf.test @@ -0,0 +1,38 @@ +nextflow_pipeline { + + name "Test pipeline for structural somatic variants, tested benchmarking methods are truvari and svbenchmark" + script "../main.nf" + tag "pipeline" + tag "somatic" + tag "structural" + config "../conf/tests/somatic_sv.config" + + test("Params: --analysis 'somatic' --variant_type 'structural' --method 'truvari,svbenchmark'") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap new file mode 100644 index 0000000..0278f7c --- /dev/null +++ b/tests/somatic_sv.nf.test.snap @@ -0,0 +1,226 @@ +{ + "Params: --analysis 'somatic' --variant_type 'structural' --method 'truvari,svbenchmark'": { + "content": [ + 69, + { + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_SORT": { + "bcftools": 1.18 + }, + "BCFTOOLS_STATS": { + "bcftools": 1.18 + }, + 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"nextflow": "24.10.0" + }, + "timestamp": "2024-11-11T14:39:35.533136259" + } +} \ No newline at end of file From 4930db37a73fbe87604b9e0cf001bab5000d70db Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 12 Nov 2024 13:21:57 +0000 Subject: [PATCH 195/292] update nftignore and snapshots --- modules.json | 2 +- .../happy/sompy/tests/main.nf.test.snap | 16 +- tests/.nftignore | 3 + tests/germline_small.nf.test.snap | 288 ++----------- tests/germline_sv.nf.test.snap | 404 +++--------------- tests/liftover_hg37.nf.test.snap | 38 +- tests/liftover_hg38.nf.test.snap | 292 ++----------- tests/somatic_indel.nf.test.snap | 8 +- tests/somatic_snv.nf.test.snap | 13 +- tests/somatic_sv.nf.test.snap | 28 +- 10 files changed, 139 insertions(+), 953 deletions(-) diff --git a/modules.json b/modules.json index c7c642f..cfd26cd 100644 --- a/modules.json +++ b/modules.json @@ -72,7 +72,7 @@ }, "happy/sompy": { "branch": "master", - "git_sha": "2f04da318223fd982f8aa3d5546dabdf42afa4eb", + "git_sha": "52e382788f0a5eedf8acee8329c775358db09720", "installed_by": ["modules"] }, "manta/convertinversion": { diff --git a/modules/nf-core/happy/sompy/tests/main.nf.test.snap b/modules/nf-core/happy/sompy/tests/main.nf.test.snap index e483f1c..8fd374a 100644 --- a/modules/nf-core/happy/sompy/tests/main.nf.test.snap +++ b/modules/nf-core/happy/sompy/tests/main.nf.test.snap @@ -6,7 +6,7 @@ { "id": "test" }, - "test.stats.csv:md5,0fa86a9726fcc3e2172342dd1305cf82" + "test.stats.csv:md5,8b35dd00bf7b5bd697a05d73fb8c0816" ] ], [ @@ -16,10 +16,10 @@ "test.features.csv" ], "meta": { - "nf-test": "0.9.1", - "nextflow": "24.04.4" + "nf-test": "0.9.2", + "nextflow": "24.10.0" }, - "timestamp": "2024-10-28T10:43:13.082233148" + "timestamp": "2024-11-11T16:03:54.153552746" }, "homo_sapiens - vcf - bam": { "content": [ @@ -28,7 +28,7 @@ { "id": "test" }, - "test.stats.csv:md5,2502fe315dcf32d00c9961b9462759aa" + "test.stats.csv:md5,5e9aae3e92641f1934c10fe88f250e59" ] ], [ @@ -38,10 +38,10 @@ "test.features.csv" ], "meta": { - "nf-test": "0.9.1", - "nextflow": "24.04.4" + "nf-test": "0.9.2", + "nextflow": "24.10.0" }, - "timestamp": "2024-10-28T10:43:32.122793995" + "timestamp": "2024-11-11T16:04:07.938866093" }, "homo_sapiens - vcf - stub": { "content": [ diff --git a/tests/.nftignore b/tests/.nftignore index 02ba6e4..e2ca67a 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -4,3 +4,6 @@ **/multiqc/multiqc_report.html pipeline_info/*.{html,json,txt,yml} **/summary/tables/* +**/summary/datavzrd/config.json +**/summary/datavzrd/index_1.html +**/**/benchmarks/**/*.{json,tsv.gz,vcf.gz,gz.tbi} diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 2171e66..4632e9e 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -208,9 +208,9 @@ { "directory": false, "file": true, - "freeSpace": 1190473797632, + "freeSpace": 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a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -296,7 +296,7 @@ "structural/test3/wittyer_bench/test3.HG002.dragen.vcf.gz.tbi" ], [ - "HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.rh.norm.dedup.sort.vcf.gz:md5,19f4c6b3ac43ad2ee4c7dc48fe7ef4bb", + "HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.rh.norm.dedup.sort.vcf.gz:md5,339c5518695913fc5c43e4bcfe902603", "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", "svbenchmark.FN.csv:md5,fda320e4b95452acedfdc8cc3a472ad3", @@ -312,11 +312,11 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,5e2eb373feb2110b83e84f7f91cdfa45", - "data_1.js:md5,542996b6d26ad1cbecd099bf28a69483", + "config.js:md5,4d9e66bb0662b5b1e6cd7e8d6c0b2386", + "data_1.js:md5,e15274b85374d74d5ed781385b3bdb7d", 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"/workspace/variantbenchmarking/.nf-test/tests/f1c1bd2313b8b668634794fad1bee4bb/output/structural/test3/preprocess/test3.dedup.sort.vcf.gz", @@ -1232,6 +932,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-11T14:10:29.600462742" + "timestamp": "2024-11-12T11:05:54.165866979" } } \ No newline at end of file diff --git a/tests/liftover_hg37.nf.test.snap b/tests/liftover_hg37.nf.test.snap index e8ebe30..b93f416 100644 --- a/tests/liftover_hg37.nf.test.snap +++ b/tests/liftover_hg37.nf.test.snap @@ -170,9 +170,9 @@ "structural/test2/stats/survivor/test2.manta.stats" ], [ - "HG002.renamechr.vcf.gz:md5,f4fdd83468f1baf6a2140a33b7884f5b", - "genome.dict:md5,63d7fa60659deebdef3aa6d6e2676b75", - "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,077f191ccbcb727e2a6839fdee132a55", + "HG002.renamechr.vcf.gz:md5,9dcf75d872d6840fffc7a366458e064b", + "genome.dict:md5,751358d3b38fc140a56462e608c41345", + "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,7c42d74914ac4dc6d1842120a54cdc9a", "HG002.bcftools_stats.txt:md5,c60ba56ddea48806895ffd3c1813d39f", "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", "truvari.FN.csv:md5,23c2da6a1f4b47445739e57adb5eaccf", @@ -186,11 +186,11 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,872ca760c42a96675f697d7e187e7531", + "config.js:md5,09c52c3dcfb90850dfe7941a0481973f", "data_1.js:md5,2ad41660c1ffd8c282add762bc5d4abf", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,5b1417eee38958b81325fce37432cf77", - "index_1.html:md5,3747fd46bd10a08c97c6faf326d6d851", + "index_1.html:md5,c3577bc8bead945d1d1d21ef7730ad11", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,b04998edbc8d3dbcab1878f5e6af76b0", "plot_2.js:md5,ca7cfcaf922101d92d8f69d7f8d53962", @@ -203,30 +203,12 @@ "metric_by_tool_truvari.png:md5,484c87060e3ecd3f72acea847415f406", "variants_by_tool_truvari.png:md5,4c01b95d2505e9f1f889aa0694de8bd4", "truvari.summary.csv:md5,71ab2d8c59c17ab1707d4eff1a71211c", - "test1.HG002.delly.fn.vcf.gz:md5,22509f095ebac0a6b117991e8ea7f329", - "test1.HG002.delly.fn.vcf.gz.tbi:md5,2074965d6cb490e55e9f545817dfe7f1", - "test1.HG002.delly.fp.vcf.gz:md5,3af206905db8f90c6973a183e9a57988", - "test1.HG002.delly.fp.vcf.gz.tbi:md5,82e3520db9d4eb379327926b96033c97", - "test1.HG002.delly.summary.json:md5,a14194fa460c135e372dc85acee307ea", - "test1.HG002.delly.tp-base.vcf.gz:md5,a67a8aa0ea2bf9bfd79a4e3c4ead5e02", - "test1.HG002.delly.tp-base.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", - "test1.HG002.delly.tp-comp.vcf.gz:md5,46c37ac79ef56dc5c281846c12d9481b", - "test1.HG002.delly.tp-comp.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", - "test1.dedup.sort.vcf.gz:md5,6bbf67c5000683f1dc1a06421eb5c1f6", - "test1.norm.filter.vcf:md5,04dcccb0101a5fe59c08f7d1ff8e22c7", + 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"test2.norm.filter.vcf:md5,313517242e4e4f5a89257ea26490e7e1", + "test2.dedup.sort.vcf.gz:md5,bffac6578e7c590981bcd7484a2d5c27", + "test2.norm.filter.vcf:md5,bb38c860701f4e6cd8156ef39cde6ced", "test2.manta.bcftools_stats.txt:md5,71700db9ad154859adef19c591da0f41", "test2.manta.stats:md5,31e3d3047f38b25ae6eba9c4bd1a4292" ] @@ -235,6 +217,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-11T14:43:38.797042471" + "timestamp": "2024-11-12T10:47:28.69325896" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index c227c35..c1f7f66 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -270,15 +270,15 @@ "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" ], [ - "HG002.renamechr.vcf.gz:md5,fe4e97dd5ea1719f27d48a3b680c3708", - "genome.dict:md5,21e9f343abd6c358f69a822937848725", - "done:md5,86e8e531982976a15363ec8abfcd4c11", - "format.log:md5,f9011fc018662ec8c950e5afe01e51b1", - "mainIndex:md5,45c54025f1064b66fc252ad60572abc8", + "HG002.renamechr.vcf.gz:md5,66cc548b5d9814467acd7f0e3b9aa8ea", + "genome.dict:md5,dc2a2ddd2b830a6cf4540f510fcef656", + "done:md5,ad13746edfa83c07fec7aff9de660b35", + "format.log:md5,83809170a2da56771b823db4a9ccd0a0", + "mainIndex:md5,15d358915935fdfc4283da0c3b98de38", "nameIndex0:md5,720ad6dfad3d1ee07a3a2bc3ed953395", "namedata0:md5,758c4a60948aed6c6a4c78323aa73ab3", "namepointer0:md5,319f9f665b338f0e218677e036637e94", - "progress:md5,5c9fe5d3e0e05a941a0cdf9cd62dc881", + "progress:md5,cdfd9f68bdfa79d85cbd7bd72aecefba", "reference.txt:md5,c9f7ef637449eb0f006d90080a130e30", "seqdata0:md5,ea8728adf43db158305ba188de66e838", "seqdata1:md5,d3470516d52b20e9477323d051846c3f", @@ -292,9 +292,9 @@ "suffixIndex0:md5,b7bb2ba061ab54c0bf07c0a941d0277a", "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", - "summary.txt:md5,13519cf54dc1ef10a9cee44cedde571d", + "summary.txt:md5,75b5ffbeff60f76dd74b7607eddb55c6", "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", - "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,73a9ca260afd63336909c6b1f0a29e97", + "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,394d5f145c53855c0e2bc96a03d68198", "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", "rtgtools.FN.csv:md5,7690798baa198e924c08e03e301148d4", "rtgtools.FP.csv:md5,35f34c2f3b37f9c4a0617d450decee92", @@ -307,11 +307,11 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,669ab12f1f6be7123996c1f07ea635aa", - "data_1.js:md5,d5b4245d99cffac9dd5cfd6e7301f3e4", + "config.js:md5,f8bbef15eb75e522924310b9c62b5fc4", + "data_1.js:md5,9ef9af688f2377e4ea423430fd9f5169", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,a28f8eeccf1b40dcdf0a6dac25dc6147", - "index_1.html:md5,def7261dae8a0b3ca4f51908ca53bbaa", + "index_1.html:md5,8bcf12f061f3cdfef2cec3d586ac0c6a", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,84aa48f7741aaba5a48cc07c9a29847e", "plot_10.js:md5,249d82bf09606974e7c1e5f3f1f272f1", @@ -338,13 +338,13 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,427ef77a05bdf895613acac07d67cb3a", + "config.js:md5,688a7474fcee4c787aecd9e0bfca800a", "data_1.js:md5,0dd5f26d5dc5901487ed185385a99bd7", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,948f8529ae473f76675d36411c378c76", - "index_1.html:md5,1259e3273eef9a210668a9552bc599e3", + "index_1.html:md5,66b8b5a527abb97dd4c71ee483297610", "plot_0.js:md5,b7f0e3251ea8ed21eed8c70da70defc6", - "plot_1.js:md5,4822e8f30420e2e662b6b2e321d0a52f", + 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--git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index cad96d0..187c2f5 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -136,11 +136,11 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,e95384afc9056c9e85f48ad17bbe7e9f", + "config.js:md5,b55917bb95097bf9c4228d66b33483af", "data_1.js:md5,fed10370d1a4569e5e50a043191f6052", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,4b54716d3562e01c8957ffea6a091346", - "index_1.html:md5,2752e30491edff8b9ab985b015312912", + "index_1.html:md5,10baf5f9b0604cb8e0fd2b33dafd083e", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,84aa48f7741aaba5a48cc07c9a29847e", "plot_10.js:md5,68400ad3129a9967e68e0f36dcc0a82e", @@ -166,11 +166,9 @@ "sompy.regions.csv:md5,3832053ae733a56579ccf1d8947dfee6", "sompy.summary.csv:md5,4ba6196c6461f2af51c4ed2b71d6ad0b", "test1.SEQC2.freebayes.features.csv:md5,41752d01679b1efee789bdf0573b6057", - "test1.SEQC2.freebayes.metrics.json:md5,4b82077b923d395296dbb75ffef63ea4", "test1.SEQC2.freebayes.stats.csv:md5,b2c32225c192f4704f6ce16dfb64dacb", "test1.freebayes.bcftools_stats.txt:md5,c5025b5c6256c1d808badee73a7c220c", "test2.SEQC2.strelka.features.csv:md5,c5af06f0e6d85e53f890826b86d2d0ec", - "test2.SEQC2.strelka.metrics.json:md5,3a280dca8f122df4d64896479c272990", "test2.SEQC2.strelka.stats.csv:md5,dbf9f81ef2d2c92c70f845bf0ed21929", "HCC1395T_vs_HCC1395N.strelka.somatic_indels.sort.vcf.gz:md5,2392f7c3d0bb613add3f90dcdfa9b04e", "test2.strelka.bcftools_stats.txt:md5,ec553881bc70c786e8cec95192823634" @@ -180,6 +178,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-11T14:16:58.534590222" + "timestamp": "2024-11-12T12:56:25.362287984" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 94457b8..fbc8ab3 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -138,18 +138,16 @@ "sompy.regions.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "sompy.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.SEQC2.freebayes.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.SEQC2.freebayes.metrics.json:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.SEQC2.freebayes.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.freebayes.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.SEQC2.manta.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.SEQC2.manta.metrics.json:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.SEQC2.manta.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", { "directory": false, "file": true, - "freeSpace": 1192700776448, + "freeSpace": 1188051619840, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", - "usableSpace": 1192700776448, + "usableSpace": 1188051619840, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", @@ -160,14 +158,13 @@ }, "test2.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.SEQC2.strelka.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.SEQC2.strelka.metrics.json:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.SEQC2.strelka.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", { "directory": false, "file": true, - "freeSpace": 1192700776448, + "freeSpace": 1188051619840, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", - "usableSpace": 1192700776448, + "usableSpace": 1188051619840, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", @@ -183,6 +180,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-11T14:36:57.021757547" + "timestamp": "2024-11-12T13:15:54.644646851" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index 0278f7c..59f6d60 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -172,11 +172,11 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,77792b92cdcc9767e56fab1cc135ba0f", + "config.js:md5,98ed6b36ed012c5aa65e75c61ec3cb54", "data_1.js:md5,56e4053c48e5858314cf3de8dac6dbe6", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,885c02000a8bb539c1a9fd6e2d960ac4", - "index_1.html:md5,2a6fae72c38492533bfd7493beff0af2", + "index_1.html:md5,a8d8f2128039044e7e3a3cd683d6f08a", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,1963fdf668e3747ceea1114ff3b291c1", "plot_2.js:md5,35ebace769c9125907fb52dc33b52a63", @@ -189,30 +189,12 @@ "metric_by_tool_truvari.png:md5,b4c3f9cf2f25fd8f3965f50e6e20e2b4", "variants_by_tool_truvari.png:md5,217213991ae219644eb26370b20cf934", "truvari.summary.csv:md5,b32bccb18529ca93336a8755adbfb58e", - "test1.SEQC2.tiddit.fn.vcf.gz:md5,c8a46622c09de222471cf94277206ee7", - "test1.SEQC2.tiddit.fn.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", - "test1.SEQC2.tiddit.fp.vcf.gz:md5,02a5f3d80ef1c1e286bf280ad43904bd", - "test1.SEQC2.tiddit.fp.vcf.gz.tbi:md5,ac46d7d3c842df5a4f8d9d68a1ea0d0b", - "test1.SEQC2.tiddit.summary.json:md5,df96cce87a1105fb5e9df59a83e8e671", - "test1.SEQC2.tiddit.tp-base.vcf.gz:md5,c8a46622c09de222471cf94277206ee7", - "test1.SEQC2.tiddit.tp-base.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", - "test1.SEQC2.tiddit.tp-comp.vcf.gz:md5,a5d6f474e1e0f9ac77257ba509464f05", - "test1.SEQC2.tiddit.tp-comp.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", "HCC1395T_vs_HCC1395N.tiddit_sv_merge.sort.vcf.gz:md5,343bbbdfd16cf89e76480c7520313f56", - "test1.filter.vcf:md5,5d4bdf05f3cf8b1251fa10197729829c", + "test1.filter.vcf:md5,7656f2afbfde001f4712b3e9f93366a2", "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", "test1.tiddit.stats:md5,0eb749429a6072f6bf63d377e37514d1", - "test2.SEQC2.manta.fn.vcf.gz:md5,c8a46622c09de222471cf94277206ee7", - "test2.SEQC2.manta.fn.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", - "test2.SEQC2.manta.fp.vcf.gz:md5,28787426e3923d27da21562a1f612919", - "test2.SEQC2.manta.fp.vcf.gz.tbi:md5,527faf4496dac3a8434737149b336846", - "test2.SEQC2.manta.summary.json:md5,224d71ce921dd3863a4889a749fdcfde", - "test2.SEQC2.manta.tp-base.vcf.gz:md5,c8a46622c09de222471cf94277206ee7", - "test2.SEQC2.manta.tp-base.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", - "test2.SEQC2.manta.tp-comp.vcf.gz:md5,79ad4166fc48b9977f2f9daa00b53ccb", - "test2.SEQC2.manta.tp-comp.vcf.gz.tbi:md5,e7180bb953d2bd657c420a5f76a7164d", "HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz:md5,4e8f2780fbaeec83203fa99b156a974b", - "test2.filter.vcf:md5,cc35443f70885fa14f8668988e002721", + "test2.filter.vcf:md5,8ec46db45095742cd2421c0d132b6708", "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", "test2.manta.stats:md5,73bc60cd2754202ddca8be6552e85ffa" ] @@ -221,6 +203,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-11T14:39:35.533136259" + "timestamp": "2024-11-12T13:20:59.230867837" } } \ No newline at end of file From aa43e80168a9db5daabf9fc2f10d6ad524da3a3b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 12 Nov 2024 14:50:56 +0000 Subject: [PATCH 196/292] updated nftignore and snapshots again --- conf/modules.config | 2 +- tests/.nftignore | 12 +- tests/germline_small.nf.test.snap | 66 +++-------- tests/germline_sv.nf.test.snap | 185 +++++++++++------------------- tests/liftover_hg37.nf.test.snap | 24 ++-- tests/liftover_hg38.nf.test.snap | 68 +++-------- tests/somatic_indel.nf.test.snap | 7 +- tests/somatic_snv.nf.test.snap | 15 +-- tests/somatic_sv.nf.test.snap | 14 +-- 9 files changed, 127 insertions(+), 266 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 9210b89..8107600 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -243,7 +243,7 @@ process { (meta.maxMatches != null) ? "--mm ${meta.maxMatches}" : '' ].join(' ').trim()} publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/wittyer_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/wittyer_bench"}, pattern: "*{.vcf.gz,tbi,json}", mode: params.publish_dir_mode ] diff --git a/tests/.nftignore b/tests/.nftignore index e2ca67a..8c66039 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -3,7 +3,11 @@ **/multiqc/multiqc_plots/{svg,pdf,png}/*.{svg,pdf,png} **/multiqc/multiqc_report.html pipeline_info/*.{html,json,txt,yml} -**/summary/tables/* -**/summary/datavzrd/config.json -**/summary/datavzrd/index_1.html -**/**/benchmarks/**/*.{json,tsv.gz,vcf.gz,gz.tbi} +**/summary/tables/**/*.csv +**/summary/datavzrd/**/config.json +**/summary/datavzrd/**/config.js +**/summary/datavzrd/**/index_1.html +**/summary/datavzrd/**/data_1.js +**/summary/comparisons/**/*.csv +**/**/benchmarks/**/*.{json,json.gz,tsv.gz,vcf.gz,gz.tbi} +**/**/preprocess/**/*.{vcf.gz,gz.tbi,vcf} diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 4632e9e..cf12a3a 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -208,9 +208,9 @@ { "directory": false, "file": true, - "freeSpace": 1230725423104, + "freeSpace": 1221032566784, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/2a96f1ddb8a9221f9bc622515e12d947/output/small/HG002/preprocess/HG002_GRCh38_CMRG_smallvar_v1.00.rh.norm.dedup.sort.vcf.gz", - "usableSpace": 1230725423104, + "usableSpace": 1221032566784, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/2a96f1ddb8a9221f9bc622515e12d947/output/small/HG002/preprocess/HG002_GRCh38_CMRG_smallvar_v1.00.rh.norm.dedup.sort.vcf.gz", @@ -221,16 +221,9 @@ }, "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "index.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-select.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-table-fixed-columns.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -239,11 +232,8 @@ "bundle.js:md5,d41d8cd98f00b204e9800998ecf8427e", "datavzrd.css:md5,d41d8cd98f00b204e9800998ecf8427e", "index.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-select.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-table-fixed-columns.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -256,12 +246,7 @@ "variants_by_tool_happy.png:md5,d41d8cd98f00b204e9800998ecf8427e", "metric_by_tool_rtgtools.png:md5,d41d8cd98f00b204e9800998ecf8427e", "variants_by_tool_rtgtools.png:md5,d41d8cd98f00b204e9800998ecf8427e", - "happy.regions.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "happy.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.regions.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.strelka.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.HG002.strelka.metrics.json.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", @@ -274,9 +259,9 @@ { "directory": false, "file": true, - "freeSpace": 1230725423104, + "freeSpace": 1221032566784, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/2a96f1ddb8a9221f9bc622515e12d947/output/small/test1/preprocess/test1.dedup.sort.vcf.gz", - "usableSpace": 1230725423104, + "usableSpace": 1221032566784, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/2a96f1ddb8a9221f9bc622515e12d947/output/small/test1/preprocess/test1.dedup.sort.vcf.gz", @@ -287,7 +272,6 @@ }, "test1.strelka.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.HG002.bcftools.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.bcftools.metrics.json.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", @@ -300,9 +284,9 @@ { "directory": false, "file": true, - "freeSpace": 1230725423104, + "freeSpace": 1221032566784, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/2a96f1ddb8a9221f9bc622515e12d947/output/small/test2/preprocess/test2.dedup.sort.vcf.gz", - "usableSpace": 1230725423104, + "usableSpace": 1221032566784, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/2a96f1ddb8a9221f9bc622515e12d947/output/small/test2/preprocess/test2.dedup.sort.vcf.gz", @@ -318,7 +302,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T10:54:23.256724969" + "timestamp": "2024-11-12T14:18:17.835567387" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -580,13 +564,13 @@ "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" ], [ - "done:md5,a4b73ae6ff4dbf2199cee4492ddd20af", - "format.log:md5,c3af2470efc2e3e934a5ed813f11e2ec", - "mainIndex:md5,27bc509118e635050b38ae8a0778feca", + "done:md5,29f08477343a433b9a13c6771b1249c5", + "format.log:md5,aa3721ce47444775d7860c53359e73a5", + "mainIndex:md5,192e3e16058cff7120932613a1f5089a", "nameIndex0:md5,720ad6dfad3d1ee07a3a2bc3ed953395", "namedata0:md5,758c4a60948aed6c6a4c78323aa73ab3", "namepointer0:md5,319f9f665b338f0e218677e036637e94", - "progress:md5,d466b8c0c8beaa5fee2e4440e5095410", + "progress:md5,990cb56442cab560ac99b7ee507e05ae", "reference.txt:md5,c9f7ef637449eb0f006d90080a130e30", "seqdata0:md5,ea8728adf43db158305ba188de66e838", "seqdata1:md5,d3470516d52b20e9477323d051846c3f", @@ -600,13 +584,9 @@ "suffixIndex0:md5,b7bb2ba061ab54c0bf07c0a941d0277a", "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", - "summary.txt:md5,6d9735e247cd4fff2ae3d3666b2b8b38", - "HG002_GRCh38_CMRG_smallvar_v1.00.rh.norm.dedup.sort.vcf.gz:md5,3aeb93b685066fee769596e5347b0692", + "summary.txt:md5,19e31e6013fd2a35388f56dc62554133", + "HG002_GRCh38_CMRG_smallvar_v1.00.rh.norm.dedup.sort.vcf.gz:md5,e803cf48677844c89422a1429031c761", "HG002.bcftools_stats.txt:md5,b215fc0030c53bc8887e28b23b97efb6", - "rtgtools.FN.csv:md5,80f23e0271742bc7400804311dd3b437", - "rtgtools.FP.csv:md5,c5229b5b7fd78d90e9a54e64885728e5", - "rtgtools.TP_base.csv:md5,aae4d65a540533faa4f275d0f7e9753a", - "rtgtools.TP_comp.csv:md5,b2d6fa254026b506ef64ccf1f12ab21a", "index.html:md5,01d8751c7e9107446222eedc86ff3190", "bootstrap-select.min.css:md5,a04495fa5762cca97a2df5b310e7a386", "bootstrap-table-fixed-columns.min.css:md5,a5f4a3d7eeb08832c3d8d59ece24db2d", @@ -614,11 +594,8 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,801e0b5c71f0994234df79407eb52c32", - "data_1.js:md5,6637978c04519556f8deb6a364528cc9", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,a28f8eeccf1b40dcdf0a6dac25dc6147", - "index_1.html:md5,ba39d34dc43a56f21d190dd252d4a830", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,84aa48f7741aaba5a48cc07c9a29847e", "plot_10.js:md5,3b5e5334558c24ea102b28c2f41a2891", @@ -645,11 +622,8 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - 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"test1.HG002.strelka.prepy.vcf.gz:md5,f4c29ff0e99663f273ce0eb781071a5d", - "test1.dedup.sort.vcf.gz:md5,0e8488403078373d81b662a5494decf8", + "test1.HG002.strelka.prepy.vcf.gz:md5,7acd484d8946e4e9d8d1baa06d764edf", + "test1.dedup.sort.vcf.gz:md5,4a4de64364fc7da962087e40e4ef1d96", "test1.strelka.bcftools_stats.txt:md5,f9f8cf5c1be9c88754888ef8cbfb06f4", "test2.HG002.bcftools.extended.csv:md5,14392c3b5a1c060d63414cfc10e670b6", - "test2.HG002.bcftools.metrics.json.gz:md5,e3835e0ae24fa4d63d1d62be44527623", "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,507b54e5b6d1f956f557b39a7dc38ff4", "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,4694d6169ce0819d36768045a7ef43d0", "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,83d1fa126072c16b0471fb8877c5c818", @@ -695,8 +665,8 @@ "test2.HG002.bcftools.summary.csv:md5,3815ea18e8fc11f198a5821dee79a3c7", "test2.HG002.bcftools.phasing.txt:md5,38920536b8c3e241e873c07ba61762e6", "test2.HG002.bcftools.summary.txt:md5,b9a0c74a8b5af4f2c1c0623b61d7cbe3", - "test2.HG002.bcftools.prepy.vcf.gz:md5,2bb2a8f42fc4d5f3b08d6c26ba047cb3", - "test2.dedup.sort.vcf.gz:md5,58c46464b10be57981d0da31c835ea97", + "test2.HG002.bcftools.prepy.vcf.gz:md5,01462144efb53774819ea04b4a30c62f", + "test2.dedup.sort.vcf.gz:md5,b042565a6aec2310eb3c618e0fce728f", "test2.bcftools.bcftools_stats.txt:md5,57aff2f0a6f830e920869b987502a343" ] ], @@ -704,6 +674,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T10:52:16.579355432" + "timestamp": "2024-11-12T14:16:08.41679416" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index 289e54a..6c2de1e 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -214,6 +214,10 @@ "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-base.vcf.gz.tbi", "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-comp.vcf.gz", "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-comp.vcf.gz.tbi", + "structural/test1/benchmarks/wittyer_bench", + "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.json", + "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.vcf.gz", + "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.vcf.gz.tbi", "structural/test1/preprocess", "structural/test1/preprocess/manta.HG002.chr21.norm.sort.vcf.gz", "structural/test1/preprocess/manta.HG002.chr21.norm.vcf.gz", @@ -225,10 +229,6 @@ "structural/test1/stats/bcftools/test1.manta.bcftools_stats.txt", "structural/test1/stats/survivor", "structural/test1/stats/survivor/test1.manta.stats", - "structural/test1/wittyer_bench", - "structural/test1/wittyer_bench/test1.HG002.manta.json", - "structural/test1/wittyer_bench/test1.HG002.manta.vcf.gz", - "structural/test1/wittyer_bench/test1.HG002.manta.vcf.gz.tbi", "structural/test2", "structural/test2/benchmarks", "structural/test2/benchmarks/svanalyzer", @@ -247,6 +247,10 @@ "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-base.vcf.gz.tbi", "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz", "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz.tbi", + "structural/test2/benchmarks/wittyer_bench", + "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.json", + "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.vcf.gz", + "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.vcf.gz.tbi", "structural/test2/preprocess", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.sort.vcf.gz", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.vcf.gz", @@ -257,10 +261,6 @@ "structural/test2/stats/bcftools/test2.merged.bcftools_stats.txt", "structural/test2/stats/survivor", "structural/test2/stats/survivor/test2.merged.stats", - "structural/test2/wittyer_bench", - "structural/test2/wittyer_bench/test2.HG002.merged.json", - "structural/test2/wittyer_bench/test2.HG002.merged.vcf.gz", - "structural/test2/wittyer_bench/test2.HG002.merged.vcf.gz.tbi", "structural/test3", "structural/test3/benchmarks", "structural/test3/benchmarks/svanalyzer", @@ -279,6 +279,10 @@ "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-base.vcf.gz.tbi", "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-comp.vcf.gz", "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-comp.vcf.gz.tbi", + "structural/test3/benchmarks/wittyer_bench", + "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.json", + "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.vcf.gz", + "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.vcf.gz.tbi", "structural/test3/preprocess", "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.sort.vcf.gz", "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", @@ -289,22 +293,12 @@ "structural/test3/stats/bcftools", "structural/test3/stats/bcftools/test3.dragen.bcftools_stats.txt", "structural/test3/stats/survivor", - "structural/test3/stats/survivor/test3.dragen.stats", - "structural/test3/wittyer_bench", - "structural/test3/wittyer_bench/test3.HG002.dragen.json", - "structural/test3/wittyer_bench/test3.HG002.dragen.vcf.gz", - "structural/test3/wittyer_bench/test3.HG002.dragen.vcf.gz.tbi" + "structural/test3/stats/survivor/test3.dragen.stats" ], [ - "HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.rh.norm.dedup.sort.vcf.gz:md5,339c5518695913fc5c43e4bcfe902603", + "HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.rh.norm.dedup.sort.vcf.gz:md5,f198683490f73c5fdfaa129501def87c", "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "svbenchmark.FN.csv:md5,fda320e4b95452acedfdc8cc3a472ad3", - "svbenchmark.FP.csv:md5,35068a0c16903cba32257a8d9a6d51a2", - "truvari.FN.csv:md5,7d2992f331488dd307831a25b8157b0a", - "truvari.FP.csv:md5,cdc258e9153f7a75fc8340ce7211b577", - "truvari.TP_base.csv:md5,cd6d6a9c870739378c7e0ecc6868ebf6", - 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"freeSpace": 1209872990208, + "freeSpace": 1213910159360, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/f1c1bd2313b8b668634794fad1bee4bb/output/structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", - "usableSpace": 1209872990208, + "usableSpace": 1213910159360, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/f1c1bd2313b8b668634794fad1bee4bb/output/structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", @@ -908,9 +854,9 @@ { "directory": false, "file": true, - "freeSpace": 1209872990208, + "freeSpace": 1213910159360, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/f1c1bd2313b8b668634794fad1bee4bb/output/structural/test3/preprocess/test3.dedup.sort.vcf.gz", - "usableSpace": 1209872990208, + "usableSpace": 1213910159360, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/f1c1bd2313b8b668634794fad1bee4bb/output/structural/test3/preprocess/test3.dedup.sort.vcf.gz", @@ -922,16 +868,13 @@ "test3.dragen.svync.rh.norm.filter.vcf:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.dragen.svync.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test3.dragen.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.dragen.stats:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.HG002.dragen.json:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.HG002.dragen.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test3.HG002.dragen.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + "test3.dragen.stats:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "meta": { "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T11:05:54.165866979" + "timestamp": "2024-11-12T14:26:35.944326229" } } \ No newline at end of file diff --git a/tests/liftover_hg37.nf.test.snap b/tests/liftover_hg37.nf.test.snap index b93f416..c3e1fcc 100644 --- a/tests/liftover_hg37.nf.test.snap +++ b/tests/liftover_hg37.nf.test.snap @@ -170,15 +170,11 @@ "structural/test2/stats/survivor/test2.manta.stats" ], [ - "HG002.renamechr.vcf.gz:md5,9dcf75d872d6840fffc7a366458e064b", - "genome.dict:md5,751358d3b38fc140a56462e608c41345", - "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,7c42d74914ac4dc6d1842120a54cdc9a", + "HG002.renamechr.vcf.gz:md5,edd07af86ef9fe1e58904b2a4fd85edd", + "genome.dict:md5,2f33ed2b6a6f8a3c93f868af34fdc712", + "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,06f3242596585a68c311b6b4decc494f", "HG002.bcftools_stats.txt:md5,c60ba56ddea48806895ffd3c1813d39f", "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "truvari.FN.csv:md5,23c2da6a1f4b47445739e57adb5eaccf", - "truvari.FP.csv:md5,d235a9d585d4359b57fc0c49020d06a7", - "truvari.TP_base.csv:md5,7745d45b8bf756a207f4a103ec08e590", - "truvari.TP_comp.csv:md5,35b9f89cc07df1f3db017669431ec97e", "index.html:md5,01d8751c7e9107446222eedc86ff3190", "bootstrap-select.min.css:md5,a04495fa5762cca97a2df5b310e7a386", "bootstrap-table-fixed-columns.min.css:md5,a5f4a3d7eeb08832c3d8d59ece24db2d", @@ -186,11 +182,8 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,09c52c3dcfb90850dfe7941a0481973f", - "data_1.js:md5,2ad41660c1ffd8c282add762bc5d4abf", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,5b1417eee38958b81325fce37432cf77", - "index_1.html:md5,c3577bc8bead945d1d1d21ef7730ad11", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,b04998edbc8d3dbcab1878f5e6af76b0", "plot_2.js:md5,ca7cfcaf922101d92d8f69d7f8d53962", @@ -202,13 +195,12 @@ "versions.yml:md5,d503c03a8d9f5141f0bc1547d4edd8ec", "metric_by_tool_truvari.png:md5,484c87060e3ecd3f72acea847415f406", "variants_by_tool_truvari.png:md5,4c01b95d2505e9f1f889aa0694de8bd4", - "truvari.summary.csv:md5,71ab2d8c59c17ab1707d4eff1a71211c", - "test1.dedup.sort.vcf.gz:md5,a8e2688e9a207071a2b4c95368c6467d", - "test1.norm.filter.vcf:md5,a0623a107197fa18c0af795e35eb3885", + "test1.dedup.sort.vcf.gz:md5,9f92214d7f469ab0947f7bd919c611f3", + "test1.norm.filter.vcf:md5,d768bfa7c575cfe235499b6003b59a52", "test1.delly.bcftools_stats.txt:md5,4f2d76bb6b8eef1c6a285e7b6a4e82cc", "test1.delly.stats:md5,e140ad55975c767578b0dd6aff58ba29", - "test2.dedup.sort.vcf.gz:md5,bffac6578e7c590981bcd7484a2d5c27", - "test2.norm.filter.vcf:md5,bb38c860701f4e6cd8156ef39cde6ced", + "test2.dedup.sort.vcf.gz:md5,c72139271f42316db44942aa739057c2", + "test2.norm.filter.vcf:md5,720f8eeacbdcb33f89f0822a536a90df", "test2.manta.bcftools_stats.txt:md5,71700db9ad154859adef19c591da0f41", "test2.manta.stats:md5,31e3d3047f38b25ae6eba9c4bd1a4292" ] @@ -217,6 +209,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T10:47:28.69325896" + "timestamp": "2024-11-12T14:41:43.201827786" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index c1f7f66..d6d8419 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -270,15 +270,15 @@ "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" ], [ - "HG002.renamechr.vcf.gz:md5,66cc548b5d9814467acd7f0e3b9aa8ea", - "genome.dict:md5,dc2a2ddd2b830a6cf4540f510fcef656", - "done:md5,ad13746edfa83c07fec7aff9de660b35", - "format.log:md5,83809170a2da56771b823db4a9ccd0a0", - "mainIndex:md5,15d358915935fdfc4283da0c3b98de38", + "HG002.renamechr.vcf.gz:md5,93eb3287bd3792d642891a2f92b6daa9", + "genome.dict:md5,6b9fba7378340a89ccf1278ce99f380c", + "done:md5,86e8e531982976a15363ec8abfcd4c11", + "format.log:md5,a0c27c2c4382b58a3b47879f7fc239e6", + "mainIndex:md5,e64ab063d211ff6f8e583ba6a8726a40", "nameIndex0:md5,720ad6dfad3d1ee07a3a2bc3ed953395", "namedata0:md5,758c4a60948aed6c6a4c78323aa73ab3", "namepointer0:md5,319f9f665b338f0e218677e036637e94", - "progress:md5,cdfd9f68bdfa79d85cbd7bd72aecefba", + "progress:md5,534e62225e8fbb7d0d41bee26453e8f7", "reference.txt:md5,c9f7ef637449eb0f006d90080a130e30", "seqdata0:md5,ea8728adf43db158305ba188de66e838", "seqdata1:md5,d3470516d52b20e9477323d051846c3f", @@ -292,14 +292,10 @@ "suffixIndex0:md5,b7bb2ba061ab54c0bf07c0a941d0277a", "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", - "summary.txt:md5,75b5ffbeff60f76dd74b7607eddb55c6", + "summary.txt:md5,f371fbe9235331a38c18b292f705dff9", "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", - "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,394d5f145c53855c0e2bc96a03d68198", + "HG002.renamechr.rh.norm.dedup.sort.vcf.gz:md5,0f7e555619c993b25bcdc1cfb5aec371", "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", - "rtgtools.FN.csv:md5,7690798baa198e924c08e03e301148d4", - "rtgtools.FP.csv:md5,35f34c2f3b37f9c4a0617d450decee92", - "rtgtools.TP_base.csv:md5,344d3777b90a06e73aa59d1cc050015a", - "rtgtools.TP_comp.csv:md5,9ed56d1b7e88177723ad7b5bf584bc43", "index.html:md5,01d8751c7e9107446222eedc86ff3190", "bootstrap-select.min.css:md5,a04495fa5762cca97a2df5b310e7a386", "bootstrap-table-fixed-columns.min.css:md5,a5f4a3d7eeb08832c3d8d59ece24db2d", @@ -307,11 +303,8 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,f8bbef15eb75e522924310b9c62b5fc4", - "data_1.js:md5,9ef9af688f2377e4ea423430fd9f5169", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,a28f8eeccf1b40dcdf0a6dac25dc6147", - "index_1.html:md5,8bcf12f061f3cdfef2cec3d586ac0c6a", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,84aa48f7741aaba5a48cc07c9a29847e", "plot_10.js:md5,249d82bf09606974e7c1e5f3f1f272f1", @@ -338,13 +331,10 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,688a7474fcee4c787aecd9e0bfca800a", - "data_1.js:md5,0dd5f26d5dc5901487ed185385a99bd7", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,948f8529ae473f76675d36411c378c76", - "index_1.html:md5,66b8b5a527abb97dd4c71ee483297610", "plot_0.js:md5,b7f0e3251ea8ed21eed8c70da70defc6", - "plot_1.js:md5,0e2e9d845d82aa6fbebdfa5a20f447a1", + "plot_1.js:md5,4822e8f30420e2e662b6b2e321d0a52f", "plot_2.js:md5,c3b2e59dc30137c6347297d318a2207d", "plot_3.js:md5,c095ad6760be6b2a669e733d35a5581d", "plot_4.js:md5,203fcdfec472bb2f3e14ba1f20a62253", @@ -363,10 +353,7 @@ "SNP_PASS_variants_by_tool_happy.png:md5,d6cef7c8865329925213063821b69d5d", "metric_by_tool_rtgtools.png:md5,022825153811ea1430a84f7b47b3014b", "variants_by_tool_rtgtools.png:md5,67282d70650518b8d10eb7679e2ffaf3", - "happy.summary.csv:md5,1c3841ab107df55183334b27e640d8e0", - "rtgtools.summary.csv:md5,1e51659529ea937b083926dbfa6b6a52", "test1.HG002.strelka.extended.csv:md5,fadf06276179c1cc388007e5651fb9af", - "test1.HG002.strelka.metrics.json.gz:md5,a547451f105fa988e08642d90bc98aec", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a08665be8ea20855a14d6418d07521dc", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8d4a20a5914379230952aeb252845e2d", "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,c546609a3e23b4c5c97ee0fc2e864e07", @@ -375,10 +362,9 @@ "test1.HG002.strelka.summary.csv:md5,001fa2371777d1d2dc3eb0f4dcaca197", "test1.HG002.strelka.phasing.txt:md5,0c9340e64e032b5f74d0ea3773464afd", "test1.HG002.strelka.summary.txt:md5,4b775edb878759727ef66d5d5b2b9ee8", - "test1.dedup.sort.vcf.gz:md5,5d4790445e0fb18b4571313fa9464e8e", + "test1.dedup.sort.vcf.gz:md5,7d1d8b823c3fc108a3f4dd873b9aefc4", "test1.strelka.bcftools_stats.txt:md5,f9f8cf5c1be9c88754888ef8cbfb06f4", "test2.HG002.bcftools.extended.csv:md5,05c6efd85a9b9823a5ddfe6619be3323", - "test2.HG002.bcftools.metrics.json.gz:md5,9d6f8df3a99c641ed11e386cefc2aa4c", "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,463cee548b7bdf133c0ae9b5803a50ca", "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,424e39c327ae6ef9001942bc895abdc9", "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,508277f9aa9a541812360bbf914ae3fc", @@ -387,7 +373,7 @@ "test2.HG002.bcftools.summary.csv:md5,38f3d8eb32c4d006a5623777ad265c39", "test2.HG002.bcftools.phasing.txt:md5,38920536b8c3e241e873c07ba61762e6", "test2.HG002.bcftools.summary.txt:md5,264eb2b064a9ee4b098470a13c2887c9", - "test2.dedup.sort.vcf.gz:md5,fc7c0b47a4fa4de5e000c4e603051011", + "test2.dedup.sort.vcf.gz:md5,18f7737250a7e5f88b6a613805041d1d", "test2.bcftools.bcftools_stats.txt:md5,57aff2f0a6f830e920869b987502a343" ] ], @@ -395,7 +381,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T10:30:00.513358743" + "timestamp": "2024-11-12T14:47:37.156306631" }, "-stub": { "content": [ @@ -620,9 +606,9 @@ { "directory": false, "file": true, - "freeSpace": 1261454155776, + "freeSpace": 1201775271936, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/bfc3efb97da9551aeb5b9f7c830cf2e/output/small/HG002/preprocess/HG002.renamechr.rh.norm.dedup.sort.vcf.gz", - "usableSpace": 1261454155776, + "usableSpace": 1201775271936, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/bfc3efb97da9551aeb5b9f7c830cf2e/output/small/HG002/preprocess/HG002.renamechr.rh.norm.dedup.sort.vcf.gz", @@ -633,16 +619,9 @@ }, "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "index.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-select.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-table-fixed-columns.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -651,11 +630,8 @@ "bundle.js:md5,d41d8cd98f00b204e9800998ecf8427e", "datavzrd.css:md5,d41d8cd98f00b204e9800998ecf8427e", "index.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-select.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-table-fixed-columns.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -668,12 +644,7 @@ "variants_by_tool_happy.png:md5,d41d8cd98f00b204e9800998ecf8427e", "metric_by_tool_rtgtools.png:md5,d41d8cd98f00b204e9800998ecf8427e", "variants_by_tool_rtgtools.png:md5,d41d8cd98f00b204e9800998ecf8427e", - "happy.regions.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "happy.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.regions.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.strelka.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.HG002.strelka.metrics.json.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", @@ -685,9 +656,9 @@ { "directory": false, "file": true, - "freeSpace": 1261454155776, + "freeSpace": 1201775271936, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/bfc3efb97da9551aeb5b9f7c830cf2e/output/small/test1/preprocess/test1.dedup.sort.vcf.gz", - "usableSpace": 1261454155776, + "usableSpace": 1201775271936, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/bfc3efb97da9551aeb5b9f7c830cf2e/output/small/test1/preprocess/test1.dedup.sort.vcf.gz", @@ -698,7 +669,6 @@ }, "test1.strelka.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.HG002.bcftools.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.bcftools.metrics.json.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", @@ -710,9 +680,9 @@ { "directory": false, "file": true, - "freeSpace": 1261454155776, + "freeSpace": 1201775271936, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/bfc3efb97da9551aeb5b9f7c830cf2e/output/small/test2/preprocess/test2.dedup.sort.vcf.gz", - "usableSpace": 1261454155776, + "usableSpace": 1201775271936, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/bfc3efb97da9551aeb5b9f7c830cf2e/output/small/test2/preprocess/test2.dedup.sort.vcf.gz", @@ -728,6 +698,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T10:32:18.394617998" + "timestamp": "2024-11-12T14:50:10.696248757" } } \ No newline at end of file diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 187c2f5..7911a83 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -136,11 +136,8 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,b55917bb95097bf9c4228d66b33483af", - "data_1.js:md5,fed10370d1a4569e5e50a043191f6052", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,4b54716d3562e01c8957ffea6a091346", - "index_1.html:md5,10baf5f9b0604cb8e0fd2b33dafd083e", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,84aa48f7741aaba5a48cc07c9a29847e", "plot_10.js:md5,68400ad3129a9967e68e0f36dcc0a82e", @@ -163,8 +160,6 @@ "versions.yml:md5,d503c03a8d9f5141f0bc1547d4edd8ec", "metric_by_tool_sompy.png:md5,27c2aefe65fd23344895343c1de228e9", "variants_by_tool_sompy.png:md5,7ba77d46cfbefac1d057e2965925ad51", - "sompy.regions.csv:md5,3832053ae733a56579ccf1d8947dfee6", - "sompy.summary.csv:md5,4ba6196c6461f2af51c4ed2b71d6ad0b", "test1.SEQC2.freebayes.features.csv:md5,41752d01679b1efee789bdf0573b6057", "test1.SEQC2.freebayes.stats.csv:md5,b2c32225c192f4704f6ce16dfb64dacb", "test1.freebayes.bcftools_stats.txt:md5,c5025b5c6256c1d808badee73a7c220c", @@ -178,6 +173,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T12:56:25.362287984" + "timestamp": "2024-11-12T14:11:30.086388856" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index fbc8ab3..28d5abf 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -120,11 +120,8 @@ "SEQC2.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", "index.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-select.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", "bootstrap-table-fixed-columns.min.css:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -135,8 +132,6 @@ "versions.yml:md5,d503c03a8d9f5141f0bc1547d4edd8ec", "metric_by_tool_sompy.png:md5,d41d8cd98f00b204e9800998ecf8427e", "variants_by_tool_sompy.png:md5,d41d8cd98f00b204e9800998ecf8427e", - "sompy.regions.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "sompy.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.SEQC2.freebayes.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.SEQC2.freebayes.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.freebayes.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -145,9 +140,9 @@ { "directory": false, "file": true, - "freeSpace": 1188051619840, + "freeSpace": 1230805327872, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", - "usableSpace": 1188051619840, + "usableSpace": 1230805327872, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", @@ -162,9 +157,9 @@ { "directory": false, "file": true, - "freeSpace": 1188051619840, + "freeSpace": 1230805327872, "canonicalPath": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", - "usableSpace": 1188051619840, + "usableSpace": 1230805327872, "hidden": false, "totalSpace": 1288479703040, "path": "/workspace/variantbenchmarking/.nf-test/tests/85debb69fdfa1154bc487cb7ffc29e7f/output/snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", @@ -180,6 +175,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T13:15:54.644646851" + "timestamp": "2024-11-12T13:57:27.157417913" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index 59f6d60..be6d418 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -161,10 +161,6 @@ [ "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", "SEQC2.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", - "truvari.FN.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", - "truvari.FP.csv:md5,d47360e5f1260a7d1cfa1797a7814ef8", - "truvari.TP_base.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", - "truvari.TP_comp.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", "index.html:md5,01d8751c7e9107446222eedc86ff3190", "bootstrap-select.min.css:md5,a04495fa5762cca97a2df5b310e7a386", "bootstrap-table-fixed-columns.min.css:md5,a5f4a3d7eeb08832c3d8d59ece24db2d", @@ -172,11 +168,8 @@ "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "config.js:md5,98ed6b36ed012c5aa65e75c61ec3cb54", - "data_1.js:md5,56e4053c48e5858314cf3de8dac6dbe6", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", "heatmap.js:md5,885c02000a8bb539c1a9fd6e2d960ac4", - "index_1.html:md5,a8d8f2128039044e7e3a3cd683d6f08a", "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", "plot_1.js:md5,1963fdf668e3747ceea1114ff3b291c1", "plot_2.js:md5,35ebace769c9125907fb52dc33b52a63", @@ -188,13 +181,12 @@ "versions.yml:md5,d503c03a8d9f5141f0bc1547d4edd8ec", "metric_by_tool_truvari.png:md5,b4c3f9cf2f25fd8f3965f50e6e20e2b4", "variants_by_tool_truvari.png:md5,217213991ae219644eb26370b20cf934", - "truvari.summary.csv:md5,b32bccb18529ca93336a8755adbfb58e", "HCC1395T_vs_HCC1395N.tiddit_sv_merge.sort.vcf.gz:md5,343bbbdfd16cf89e76480c7520313f56", - "test1.filter.vcf:md5,7656f2afbfde001f4712b3e9f93366a2", + "test1.filter.vcf:md5,510eb4a0d9a07a889f1b5587fe44e557", "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", "test1.tiddit.stats:md5,0eb749429a6072f6bf63d377e37514d1", "HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz:md5,4e8f2780fbaeec83203fa99b156a974b", - "test2.filter.vcf:md5,8ec46db45095742cd2421c0d132b6708", + "test2.filter.vcf:md5,4a3ffa00cbc58758de82fc619710501f", "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", "test2.manta.stats:md5,73bc60cd2754202ddca8be6552e85ffa" ] @@ -203,6 +195,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T13:20:59.230867837" + "timestamp": "2024-11-12T13:51:00.790106674" } } \ No newline at end of file From 7d15cb25f74d595579e15000c58b003978ddbb95 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 12 Nov 2024 16:28:54 +0000 Subject: [PATCH 197/292] add more files to nftignore --- .nf-core.yml | 3 +- conf/modules.config | 2 +- tests/.nftignore | 21 ++- tests/germline_small.nf.test.snap | 141 +--------------- tests/germline_sv.nf.test.snap | 268 ++---------------------------- tests/liftover_hg37.nf.test.snap | 36 +--- tests/liftover_hg38.nf.test.snap | 149 ++--------------- tests/somatic_indel.nf.test.snap | 34 +--- tests/somatic_snv.nf.test.snap | 43 +---- tests/somatic_sv.nf.test.snap | 26 +-- 10 files changed, 60 insertions(+), 663 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 36a5b95..79752ea 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,5 +1,6 @@ bump_version: null -lint: null +lint: + actions_ci: false nf_core_version: 3.0.1 org_path: null repository_type: pipeline diff --git a/conf/modules.config b/conf/modules.config index 8107600..5528e7a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -322,7 +322,7 @@ process { ext.prefix = {"${meta.id}.renamechr"} ext.args = {"--output-type z"} publishDir = [ - path: {"${params.outdir}/${params.truth_id}/liftover"}, + path: {"${params.outdir}/${params.variant_type}/${params.truth_id}/liftover"}, pattern: "*{.vcf.gz}", mode: params.publish_dir_mode ] diff --git a/tests/.nftignore b/tests/.nftignore index 8c66039..d4e1d65 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -3,11 +3,22 @@ **/multiqc/multiqc_plots/{svg,pdf,png}/*.{svg,pdf,png} **/multiqc/multiqc_report.html pipeline_info/*.{html,json,txt,yml} +references/dictionary/genome.dict +references/rtgtools/genome.sdf/summary.txt +references/rtgtools/genome.sdf/progress +references/rtgtools/genome.sdf/format.log +references/rtgtools/genome.sdf/mainIndex **/summary/tables/**/*.csv -**/summary/datavzrd/**/config.json -**/summary/datavzrd/**/config.js -**/summary/datavzrd/**/index_1.html -**/summary/datavzrd/**/data_1.js +**/summary/datavzrd/versions.yml +**/summary/datavzrd/**/index.html +**/summary/datavzrd/**/static/* +**/summary/datavzrd/**/test/*.js +**/summary/datavzrd/**/test/index_1.html +**/summary/datavzrd/**/test/data/data_1.js +**/summary/datavzrd/**/test/plots/*.js +**/summary/plots/**/*.png **/summary/comparisons/**/*.csv **/**/benchmarks/**/*.{json,json.gz,tsv.gz,vcf.gz,gz.tbi} -**/**/preprocess/**/*.{vcf.gz,gz.tbi,vcf} +**/**/benchmarks/svanalyzer/*.{log} +**/**/preprocess/*.{vcf.gz,gz.tbi,vcf} +**/**/liftover/*.{vcf.gz,gz.tbi,vcf} diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index cf12a3a..042771a 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -205,47 +205,20 @@ ], [ "genome.sdf:md5,d41d8cd98f00b204e9800998ecf8427e", - 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"timestamp": "2024-11-12T13:57:27.157417913" + "timestamp": "2024-11-12T16:24:28.108507744" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index be6d418..331d865 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -161,32 +161,8 @@ [ "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", "SEQC2.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", - "index.html:md5,01d8751c7e9107446222eedc86ff3190", - "bootstrap-select.min.css:md5,a04495fa5762cca97a2df5b310e7a386", - "bootstrap-table-fixed-columns.min.css:md5,a5f4a3d7eeb08832c3d8d59ece24db2d", - "bootstrap-table.min.css:md5,3d1a5af7ebee2e0d5d8dc8e04493b2c6", - "bootstrap.min.css:md5,816af0eddd3b4822c2756227c7e7b7ee", - "bundle.js:md5,991c8d5442045b8afc071b4eee9b1796", - "datavzrd.css:md5,dfad5e534ff98d8d7642f080cd7d0ff5", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,885c02000a8bb539c1a9fd6e2d960ac4", - "plot_0.js:md5,dbe8a6f9908a3b27cb37296a68f8848f", - "plot_1.js:md5,1963fdf668e3747ceea1114ff3b291c1", - "plot_2.js:md5,35ebace769c9125907fb52dc33b52a63", - "plot_3.js:md5,c52694fef1000a3d6db5cf760ae4463e", - "plot_4.js:md5,172b01dbc6ddf5cf142c47655459a40d", - "plot_5.js:md5,84e85e8b4a2c5ade8986bcc29fe2b3d3", - "plot_6.js:md5,ebca08a2054e0c93bc57285274346ea4", - "plot_7.js:md5,64477cb0971b929ba244e207c055a2a0", - "versions.yml:md5,d503c03a8d9f5141f0bc1547d4edd8ec", - "metric_by_tool_truvari.png:md5,b4c3f9cf2f25fd8f3965f50e6e20e2b4", - "variants_by_tool_truvari.png:md5,217213991ae219644eb26370b20cf934", - "HCC1395T_vs_HCC1395N.tiddit_sv_merge.sort.vcf.gz:md5,343bbbdfd16cf89e76480c7520313f56", - "test1.filter.vcf:md5,510eb4a0d9a07a889f1b5587fe44e557", "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", "test1.tiddit.stats:md5,0eb749429a6072f6bf63d377e37514d1", - "HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz:md5,4e8f2780fbaeec83203fa99b156a974b", - "test2.filter.vcf:md5,4a3ffa00cbc58758de82fc619710501f", "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", "test2.manta.stats:md5,73bc60cd2754202ddca8be6552e85ffa" ] @@ -195,6 +171,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T13:51:00.790106674" + "timestamp": "2024-11-12T16:27:02.901069711" } } \ No newline at end of file From 17b4a08743edaa07c6d936e363651ccd22cd1838 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 13 Nov 2024 09:04:39 +0000 Subject: [PATCH 198/292] last update - hope --- tests/.nftignore | 1 + tests/germline_small.nf.test.snap | 3 +-- tests/liftover_hg38.nf.test | 31 ------------------------------- tests/liftover_hg38.nf.test.snap | 3 +-- 4 files changed, 3 insertions(+), 35 deletions(-) diff --git a/tests/.nftignore b/tests/.nftignore index d4e1d65..148cde2 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -8,6 +8,7 @@ references/rtgtools/genome.sdf/summary.txt references/rtgtools/genome.sdf/progress references/rtgtools/genome.sdf/format.log references/rtgtools/genome.sdf/mainIndex +references/rtgtools/genome.sdf/done **/summary/tables/**/*.csv **/summary/datavzrd/versions.yml **/summary/datavzrd/**/index.html diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 042771a..500de1a 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -507,7 +507,6 @@ "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" ], [ - "done:md5,1348e6a101bdcc2f2b1fb5d9a2d39fa2", "nameIndex0:md5,720ad6dfad3d1ee07a3a2bc3ed953395", "namedata0:md5,758c4a60948aed6c6a4c78323aa73ab3", "namepointer0:md5,319f9f665b338f0e218677e036637e94", @@ -551,6 +550,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T16:07:41.455769086" + "timestamp": "2024-11-13T08:41:21.414009313" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test b/tests/liftover_hg38.nf.test index fd3bd6a..00d46af 100644 --- a/tests/liftover_hg38.nf.test +++ b/tests/liftover_hg38.nf.test @@ -17,37 +17,6 @@ nextflow_pipeline { } } - then { - // stable_name: All files + folders in ${params.outdir}/ with a stable name - def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) - // stable_path: All files in ${params.outdir}/ with stable content - def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') - assertAll( - { assert workflow.success }, - { assert snapshot( - // Number of successful tasks - workflow.trace.succeeded().size(), - // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions - removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), - // All stable path name, with a relative path - stable_name, - // All files with stable contents - stable_path - ).match() } - ) - } - } - test("-stub") { - - options "-stub" - - when { - params { - outdir = "$outputDir" - - } - } - then { // stable_name: All files + folders in ${params.outdir}/ with a stable name def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index 9f9e528..bde2593 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -268,7 +268,6 @@ "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" ], [ - "done:md5,095c6e81d56a0e3fe969e635ed49ff3b", "nameIndex0:md5,720ad6dfad3d1ee07a3a2bc3ed953395", "namedata0:md5,758c4a60948aed6c6a4c78323aa73ab3", "namepointer0:md5,319f9f665b338f0e218677e036637e94", @@ -313,7 +312,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.0" }, - "timestamp": "2024-11-12T15:46:02.878306442" + "timestamp": "2024-11-13T09:00:38.410760165" }, "-stub": { "content": [ From 54b6db7d5ac3de3248a81e0dd5ed5396f3e352a6 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 13 Nov 2024 09:18:54 +0000 Subject: [PATCH 199/292] remove liftover hg37 --- tests/germline_sv.nf.test | 2 +- tests/liftover_hg37.nf.test | 40 ------- tests/liftover_hg37.nf.test.snap | 186 ------------------------------- tests/somatic_indel.nf.test | 2 +- 4 files changed, 2 insertions(+), 228 deletions(-) delete mode 100644 tests/liftover_hg37.nf.test delete mode 100644 tests/liftover_hg37.nf.test.snap diff --git a/tests/germline_sv.nf.test b/tests/germline_sv.nf.test index 433e02d..1ace554 100644 --- a/tests/germline_sv.nf.test +++ b/tests/germline_sv.nf.test @@ -1,6 +1,6 @@ nextflow_pipeline { - name "Test pipeline for small germline variants, tested benchmarking methods are truvari, svbenchmark and wittyer" + name "Test pipeline for structural germline variants, tested benchmarking methods are truvari, svbenchmark and wittyer" script "../main.nf" tag "pipeline" tag "germline" diff --git a/tests/liftover_hg37.nf.test b/tests/liftover_hg37.nf.test deleted file mode 100644 index f68fc24..0000000 --- a/tests/liftover_hg37.nf.test +++ /dev/null @@ -1,40 +0,0 @@ -nextflow_pipeline { - - name "Test pipeline for small germline variants, tested benchmarking methods are truvari" - script "../main.nf" - tag "pipeline" - tag "structural" - tag "germline" - tag "liftover" - tag "hg37" - config "../conf/tests/liftover_hg37.config" - - test("Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover") { - - when { - params { - outdir = "$outputDir" - } - } - - then { - // stable_name: All files + folders in ${params.outdir}/ with a stable name - def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) - // stable_path: All files in ${params.outdir}/ with stable content - def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') - assertAll( - { assert workflow.success }, - { assert snapshot( - // Number of successful tasks - workflow.trace.succeeded().size(), - // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions - removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), - // All stable path name, with a relative path - stable_name, - // All files with stable contents - stable_path - ).match() } - ) - } - } -} diff --git a/tests/liftover_hg37.nf.test.snap b/tests/liftover_hg37.nf.test.snap deleted file mode 100644 index d271641..0000000 --- a/tests/liftover_hg37.nf.test.snap +++ /dev/null @@ -1,186 +0,0 @@ -{ - "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover": { - "content": [ - 82, - { - "BCFTOOLS_DEDUP": { - "bcftools": 1.18 - }, - "BCFTOOLS_NORM": { - "bcftools": 1.18 - }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.18 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 - }, - "BGZIP_TABIX": { - "tabix": 1.12 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "MERGE_REPORTS": { - "python": "3.8.6" - }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "SURVIVOR_MERGE": { - "survivor": "1.0.7" - }, - "SURVIVOR_STATS": { - "survivor": "1.0.7" - }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, - "TABIX_TABIX": { - "tabix": "1.19.1" - }, - "TRUVARI_BENCH": { - "truvari": "4.1.0)" - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, - [ - "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", - "references", - "references/dictionary", - "references/dictionary/genome.dict", - "structural", - "structural/HG002", - "structural/HG002/liftover", - "structural/HG002/liftover/HG002.renamechr.vcf.gz", - "structural/HG002/preprocess", - "structural/HG002/preprocess/HG002.renamechr.rh.norm.dedup.sort.vcf.gz", - "structural/HG002/stats", - "structural/HG002/stats/bcftools", - "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", - "structural/HG002/stats/survivor", - "structural/HG002/stats/survivor/HG002.stats", - "structural/multiqc", - "structural/multiqc/multiqc_data", - "structural/multiqc/multiqc_data/multiqc.log", - "structural/multiqc/multiqc_data/multiqc_citations.txt", - "structural/multiqc/multiqc_data/multiqc_data.json", - "structural/multiqc/multiqc_data/multiqc_software_versions.txt", - "structural/multiqc/multiqc_data/multiqc_sources.txt", - "structural/multiqc/multiqc_report.html", - "structural/summary", - "structural/summary/comparisons", - "structural/summary/comparisons/structural", - "structural/summary/comparisons/structural/truvari.FN.csv", - "structural/summary/comparisons/structural/truvari.FP.csv", - "structural/summary/comparisons/structural/truvari.TP_base.csv", - "structural/summary/comparisons/structural/truvari.TP_comp.csv", - "structural/summary/datavzrd", - "structural/summary/datavzrd/truvari", - "structural/summary/datavzrd/truvari/index.html", - "structural/summary/datavzrd/truvari/static", - "structural/summary/datavzrd/truvari/static/bootstrap-select.min.css", - "structural/summary/datavzrd/truvari/static/bootstrap-table-fixed-columns.min.css", - "structural/summary/datavzrd/truvari/static/bootstrap-table.min.css", - "structural/summary/datavzrd/truvari/static/bootstrap.min.css", - "structural/summary/datavzrd/truvari/static/bundle.js", - "structural/summary/datavzrd/truvari/static/datavzrd.css", - "structural/summary/datavzrd/truvari/test", - "structural/summary/datavzrd/truvari/test/config.js", - "structural/summary/datavzrd/truvari/test/data", - "structural/summary/datavzrd/truvari/test/data/data_1.js", - "structural/summary/datavzrd/truvari/test/functions.js", - "structural/summary/datavzrd/truvari/test/heatmap.js", - "structural/summary/datavzrd/truvari/test/index_1.html", - "structural/summary/datavzrd/truvari/test/plots", - "structural/summary/datavzrd/truvari/test/plots/plot_0.js", - "structural/summary/datavzrd/truvari/test/plots/plot_1.js", - "structural/summary/datavzrd/truvari/test/plots/plot_2.js", - "structural/summary/datavzrd/truvari/test/plots/plot_3.js", - "structural/summary/datavzrd/truvari/test/plots/plot_4.js", - "structural/summary/datavzrd/truvari/test/plots/plot_5.js", - "structural/summary/datavzrd/truvari/test/plots/plot_6.js", - "structural/summary/datavzrd/truvari/test/plots/plot_7.js", - "structural/summary/datavzrd/versions.yml", - "structural/summary/plots", - "structural/summary/plots/truvari", - "structural/summary/plots/truvari/metric_by_tool_truvari.png", - "structural/summary/plots/truvari/variants_by_tool_truvari.png", - "structural/summary/tables", - "structural/summary/tables/truvari", - "structural/summary/tables/truvari/truvari.summary.csv", - "structural/test1", - "structural/test1/benchmarks", - "structural/test1/benchmarks/truvari", - "structural/test1/benchmarks/truvari/test1.HG002.delly.fn.vcf.gz", - "structural/test1/benchmarks/truvari/test1.HG002.delly.fn.vcf.gz.tbi", - "structural/test1/benchmarks/truvari/test1.HG002.delly.fp.vcf.gz", - "structural/test1/benchmarks/truvari/test1.HG002.delly.fp.vcf.gz.tbi", - "structural/test1/benchmarks/truvari/test1.HG002.delly.summary.json", - "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-base.vcf.gz", - "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-base.vcf.gz.tbi", - "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz", - "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz.tbi", - "structural/test1/preprocess", - "structural/test1/preprocess/test1.dedup.sort.vcf.gz", - "structural/test1/preprocess/test1.norm.filter.vcf", - "structural/test1/stats", - "structural/test1/stats/bcftools", - "structural/test1/stats/bcftools/test1.delly.bcftools_stats.txt", - "structural/test1/stats/survivor", - "structural/test1/stats/survivor/test1.delly.stats", - "structural/test2", - "structural/test2/benchmarks", - "structural/test2/benchmarks/truvari", - "structural/test2/benchmarks/truvari/test2.HG002.manta.fn.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.manta.fn.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.manta.fp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.manta.fp.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.manta.summary.json", - "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-base.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-base.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz.tbi", - "structural/test2/preprocess", - "structural/test2/preprocess/test2.dedup.sort.vcf.gz", - "structural/test2/preprocess/test2.norm.filter.vcf", - "structural/test2/stats", - "structural/test2/stats/bcftools", - "structural/test2/stats/bcftools/test2.manta.bcftools_stats.txt", - "structural/test2/stats/survivor", - "structural/test2/stats/survivor/test2.manta.stats" - ], - [ - "HG002.bcftools_stats.txt:md5,c60ba56ddea48806895ffd3c1813d39f", - "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "test1.delly.bcftools_stats.txt:md5,4f2d76bb6b8eef1c6a285e7b6a4e82cc", - "test1.delly.stats:md5,e140ad55975c767578b0dd6aff58ba29", - "test2.manta.bcftools_stats.txt:md5,71700db9ad154859adef19c591da0f41", - "test2.manta.stats:md5,31e3d3047f38b25ae6eba9c4bd1a4292" - ] - ], - "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.0" - }, - "timestamp": "2024-11-12T15:30:56.956725614" - } -} diff --git a/tests/somatic_indel.nf.test b/tests/somatic_indel.nf.test index bba5bae..9901eac 100644 --- a/tests/somatic_indel.nf.test +++ b/tests/somatic_indel.nf.test @@ -1,6 +1,6 @@ nextflow_pipeline { - name "Test pipeline for small somatic variants, tested benchmarking methods are sompy" + name "Test pipeline for indel somatic variants, tested benchmarking methods are sompy" script "../main.nf" tag "pipeline" tag "somatic" From 50eddde7150ecba3dfc2d5db7092b3e2a71e200b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 13 Nov 2024 11:17:17 +0100 Subject: [PATCH 200/292] Update .github/workflows/ci.yml correct repo name Co-authored-by: Maxime U Garcia --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index b9b76dd..161cf12 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -26,7 +26,7 @@ jobs: test: name: "${{ matrix.NXF_VER }} | ${{ matrix.profile }} | ${{ matrix.shard }}/6" # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/demultiplex') }}" + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/variantbenchmarking') }}" runs-on: ubuntu-latest strategy: fail-fast: false From 7eb375fde0bc9f63eb7f17f530dcacb87ba10a16 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 19 Nov 2024 09:10:30 +0100 Subject: [PATCH 201/292] Update nf-test.config Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- nf-test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nf-test.config b/nf-test.config index 0bf7ea8..a68d97a 100644 --- a/nf-test.config +++ b/nf-test.config @@ -1,6 +1,6 @@ config { // Location of nf-tests - testsDir "tests" + testsDir "." // nf-test directory used to create temporary files for each test workDir System.getenv("NFT_WORKDIR") ?: ".nf-test" From 2e6976d370247cffc685f2756b7645be0df67ce2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 19 Nov 2024 14:12:12 +0000 Subject: [PATCH 202/292] fixes in documentation --- README.md | 14 ++--- conf/modules.config | 2 +- conf/test_full.config | 5 +- conf/tests/germline_small.config | 2 +- conf/tests/germline_sv.config | 2 +- conf/tests/liftover_hg37.config | 2 +- conf/tests/liftover_hg38.config | 2 +- conf/tests/somatic_indel.config | 2 +- conf/tests/somatic_snv.config | 2 +- conf/tests/somatic_sv.config | 2 +- docs/output.md | 36 ++++++------ docs/truth.md | 55 ++++++++++++++++++ docs/usage.md | 99 ++++++++++++++------------------ nextflow.config | 15 +++-- 14 files changed, 143 insertions(+), 97 deletions(-) create mode 100644 docs/truth.md diff --git a/README.md b/README.md index c8092ad..9478981 100644 --- a/README.md +++ b/README.md @@ -19,7 +19,7 @@ ## Introduction -**nf-core/variantbenchmarking** is designed to evaluate and validate the accuracy of variant calling methods in genomic research especially for Human data. Initially, the pipeline is tuned well for available gold standard truth sets (for example, Genome in a Bottle and SEQC2 samples) but it can be used to compare any two variant calling results. The workflow provides benchmarking tools for small variants including SNVs and INDELs, Structural Variants (SVs) and Copy Number Variations (CNVs) for germline and somatic analysis. +**nf-core/variantbenchmarking** is designed to evaluate and validate the accuracy of variant calling methods in genomic research. Initially, the pipeline is tuned well for available gold standard truth sets (for example, Genome in a Bottle and SEQC2 samples) but it can be used to compare any two variant calling results. The workflow provides benchmarking tools for small variants including SNVs and INDELs, Structural Variants (SVs) and Copy Number Variations (CNVs) for germline and somatic analysis. The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! @@ -113,15 +113,15 @@ First, prepare a samplesheet with your input data that looks as follows: `samplesheet.csv`: ```csv -id,test_vcf,caller,vartype -test1,test1.vcf.gz,delly,sv -test2,test2.vcf,gatk,small -test3,test3.vcf.gz,cnvkit,cnv +id,test_vcf,caller +test1,test1.vcf.gz,delly +test2,test2.vcf,gatk +test3,test3.vcf.gz,cnvkit ``` -Each row represents a vcf file (test-query file). For each vcf file, variant calling method (caller) and variant type (vartype) have to be defined. +Each row represents a vcf file (test-query file). For each vcf file and variant calling method (caller) have to be defined. -User has to define or provide truth vcf in config files. There are readily available vcf files for benchmarking from Genome in a bottle and SEQC2 studies which can be used readily. +User has to define or provide truth vcf in config files. There are readily available vcf files for benchmarking from Genome in a bottle and SEQC2 studies which can be used readily. Please find detailed information about truth files [here](https://nf-co.re/variantbenchmarking/truth) For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). diff --git a/conf/modules.config b/conf/modules.config index 5528e7a..dcce096 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -295,7 +295,7 @@ process { withName: VCF_TO_CSV { ext.prefix = {"${meta.id}.${meta.tag}"} publishDir = [ - path: {"${params.outdir}/${params.variant_type}/summary/comparisons/${meta.vartype}"}, + path: {"${params.outdir}/${params.variant_type}/summary/comparisons/"}, pattern: "*{.csv}", mode: params.publish_dir_mode ] diff --git a/conf/test_full.config b/conf/test_full.config index 1e44e45..60a139c 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -20,19 +20,18 @@ params { max_time = '8.h' // Input data - input = 'assets/samplesheet_small.csv' + input = 'assets/samplesheet_full_small.csv' outdir = 'results' // Genome references genome = 'GRCh37' analysis = 'germline' - truth_id = "HG002" variant_type = "small" method = 'happy,rtgtools' preprocess = "normalization,deduplication,filter_contigs" + truth_id = "HG002" truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.vcf.gz" regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.bed" - itruth_ignore = true } diff --git a/conf/tests/germline_small.config b/conf/tests/germline_small.config index 5c09764..8dcd613 100644 --- a/conf/tests/germline_small.config +++ b/conf/tests/germline_small.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, --outdir + nextflow run nf-core/variantbenchmarking -profile germline_small, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config index 615f5c4..f6b60b7 100644 --- a/conf/tests/germline_sv.config +++ b/conf/tests/germline_sv.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, --outdir + nextflow run nf-core/variantbenchmarking -profile germline_sv, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/tests/liftover_hg37.config b/conf/tests/liftover_hg37.config index ae4957e..ac9bc2a 100644 --- a/conf/tests/liftover_hg37.config +++ b/conf/tests/liftover_hg37.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile test_liftover, --outdir + nextflow run nf-core/benchmark -profile liftover_hg37, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/tests/liftover_hg38.config b/conf/tests/liftover_hg38.config index 25a0e58..561d19e 100644 --- a/conf/tests/liftover_hg38.config +++ b/conf/tests/liftover_hg38.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile test_liftover, --outdir + nextflow run nf-core/benchmark -profile liftover_hg38, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/tests/somatic_indel.config b/conf/tests/somatic_indel.config index fbd1dc2..463b096 100644 --- a/conf/tests/somatic_indel.config +++ b/conf/tests/somatic_indel.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir + nextflow run nf-core/variantbenchmarking -profile somatic_indel, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/tests/somatic_snv.config b/conf/tests/somatic_snv.config index 09f3884..6b80903 100644 --- a/conf/tests/somatic_snv.config +++ b/conf/tests/somatic_snv.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir + nextflow run nf-core/variantbenchmarking -profile somatic_snv, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/tests/somatic_sv.config b/conf/tests/somatic_sv.config index 5d75ac5..02eeec2 100644 --- a/conf/tests/somatic_sv.config +++ b/conf/tests/somatic_sv.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile test_somatic, --outdir + nextflow run nf-core/variantbenchmarking -profile somatic_sv, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/docs/output.md b/docs/output.md index d36fd14..66df667 100644 --- a/docs/output.md +++ b/docs/output.md @@ -13,18 +13,18 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Preprocesses ](#preprocesses) - [Liftover of truth sets](#liftover) - [Input vcf statistics](#stats) -- [Benchmarking](#bench) - - [Truvari](#truvari_bench) - - [SVanalyzer](#svanalyzer_bench) - - [Wittyer](#wittyer_bench) - - [RTG-tools](#rtgtools_bench) - - [Happy](#happy_bench) - - [Sompy](#sompy_bench) +- [Benchmarking](#benchmarks) + - [Truvari](#truvari) + - [SVanalyzer](#svanalyzer) + - [Wittyer](#wittyer) + - [RTG-tools](#rtgtools) + - [Happy](#happy) + - [Sompy](#sompyh) - [Summary statistics](#summary) - [Comparison of benchmarking results](#comparisons) - [Merged summary benchmark statistics](#tables) - [Plots](#plots) - - [datavzrd HTML reports](#html) + - [datavzrd HTML reports](#datavzrd) - [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution @@ -75,7 +75,7 @@ bcftools stats applied into all variant types while survivor stats is only avail
    Output files -- `truvari_bench/` +- `truvari/` - `*.fn.vcf.gz` : False negative calls from comparison - `*.fn.vcf.gz.tbi` : False negative calls from comparison - index file - `*.fp.vcf.gz`: False positive calls from comparison @@ -85,17 +85,17 @@ bcftools stats applied into all variant types while survivor stats is only avail - `*.tp-base.vcf.gz`: True positive calls form the base VCF - `*.tp-base.vcf.gz.tbi`: True positive calls form the base VCF - index file - `*.summary.json`: Json output of performance stats -- `svanalyzer_bench/` +- `svanalyzer/` - `*.distances`: Distances for comparisons - `*.falsenegatives.vcf.gz` : False negative calls from comparison - `*.falsepositives.vcf.gz`: False positive calls from comparison - `*.log`: Log of the run - `*.report`: Output report of performance stats -- `wittyer_bench/` +- `wittyer/` - `*.vcf.gz`: Calls from comparison - `*.vcf.gz.tbi`: Calls from comparison - index file - `*.json`: Json output of performance stats -- `rtgtools_bench/` +- `rtgtools/` - `*.vcf.gz`: Calls from comparison - `*.vcf.gz.tbi`: Calls from comparison - index file - `*.fn.vcf.gz` : Contains variants from the baseline VCF which were not correctly called @@ -111,7 +111,7 @@ bcftools stats applied into all variant types while survivor stats is only avail - `*.snp_roc.tsv.gz`: Contains ROC data derived from only those variants which were represented as SNPs - `*.summary.txt`: Output summary of performance stats - `*.weighted_roc.tsv.gz`: Contains ROC data derived from all analyzed call variants, regardless of their representation -- `happy_bench/` +- `happy/` - `*.extended.csv`: Extended statistics - `*.metrics.json.gz`: JSON file containing all computed metrics and tables - `*.roc.all.csv.gz`: All precision / recall data points that were calculated @@ -123,7 +123,7 @@ bcftools stats applied into all variant types while survivor stats is only avail - `*.summary.csv`: Output summary of performance stats - `*.vcf.gz`: Calls from comparison - `*.vcf.gz.tbi`: Calls from comparison - index file -- `sompy_bench/` +- `sompy/` - `*.features.csv`: Calls from comparison - `*.metrics.json`: JSON file containing all computed metrics and tables - `*.stats.csv`: Output summary of performance stats @@ -200,11 +200,15 @@ Benchmark results are created separately for each test vcf and for each method u - `snv/` - `sompy.snv.summary.csv`: Summary of performance stats from callers - `sompy.snv.regions.csv`: Summary of performance stats split by region bins from callers -- ## `html/` +- ## `datavzrd/`
    +Output files -Note that comparison results for happy and wittyer is missing since their output does not have FP/TP/FN called variants separably. For svbenchmark, TP_base and TP_comp are also missing from the same reason. +- benchmark tool + - static + - test + - index.html: HTML file to open in the browser for datavzrd spedific tables and visualizations. ### References diff --git a/docs/truth.md b/docs/truth.md new file mode 100644 index 0000000..0222fb2 --- /dev/null +++ b/docs/truth.md @@ -0,0 +1,55 @@ +# nf-core/variantbenchmarking: Truth files + +## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/variantbenchmarking/truth](https://nf-co.re/variantbenchmarking/truth) + +## Defining Truth VCF and High confidence BED files + +This pipeline requires a set of Truth VCF, as a baseline for comparisons, and a high confidence bed files, to restrict analysis to regions. Although, those sets can be anything depending on the type of the analysis, for benchmarking of human genomes there are golden set of samples provided by [Genome in a Bottle project](https://www.nist.gov/programs-projects/genome-bottle) and [SEQC2 consortium](https://sites.google.com/view/seqc2/home/data-analysis/high-confidence-somatic-snv-and-indel-v1-2). + +Below, please find some set truths can be used for analysis: + +- params.analysis == 'germline' + + - params.genome == 'GRCh38' + + - params.vartype == 'small' + + truth_id = "HG002" + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh38/HG002_GRCh38_1_22_v4.2.1_benchmark.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh38/HG002_GRCh38_1_22_v4.2.1_benchmark_noinconsistent.bed" + + - params.genome == 'GRCh37' + + - params.vartype == 'small' + + truth_id = "HG002" + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.bed" + + - params.vartype == 'structural' + + truth_id = "HG002" + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/HG002_SVs_Tier1_v0.6.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/HG002_SVs_Tier1_v0.6.bed" + +- params.analysis == 'somatic' + + - params.genome == 'GRCh38' + + - params.vartype == 'structural' + + truth_id = "HG002" + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/CMRG_v1.00/GRCh38/StructuralVariant/HG002_GRCh38_CMRG_SV_v1.00.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/CMRG_v1.00/GRCh38/StructuralVariant/HG002_GRCh38_CMRG_SV_v1.00.bed" + + - params.vartype == 'snv' + + truth_id = "SEQC2" + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/high-confidence_sSNV_in_HC_regions_v1.2.1.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/High-Confidence_Regions_v1.2.bed" + + - params.vartype == 'indel' + + truth_id = "SEQC2" + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/high-confidence_sINDEL_in_HC_regions_v1.2.1.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/latest/High-Confidence_Regions_v1.2.bed" diff --git a/docs/usage.md b/docs/usage.md index c2cc283..58451bc 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -8,9 +8,13 @@ +Given test vcfs in samplesheet.csv, this pipelines compares them to truth vcf provided with params.truth_vcf. + +params.variant*type can be "small" or "structural" for params.analysis of "germline" or params.variant_type can be "snv", "indel" or "structural" for params.analysis of "somatic". Please be aware that \_only one type of varian_analysis is possible for each run*. + ## Samplesheet input -You will need to create a samplesheet with information about the test vcf you would like to analyze before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 4 columns, and a header row as shown in the examples below. +You will need to create a samplesheet with information about the test vcf you would like to analyze before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row as shown in the examples below. ```bash --input '[path to samplesheet file]' @@ -21,10 +25,10 @@ You will need to create a samplesheet with information about the test vcf you wo The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below. ```csv title="samplesheet.csv" -id,test_vcf,caller,vartype -test1,test1.vcf.gz,delly,sv -test2,test2.vcf,gatk,small -test3,test3.vcf.gz,cnvkit,cnv +id,test_vcf,caller +test1,test1.vcf.gz,delly +test2,test2.vcf,gatk +test3,test3.vcf.gz,cnvkit ``` | Column | Description | @@ -32,44 +36,12 @@ test3,test3.vcf.gz,cnvkit,cnv | `id` | Custom id name per test vcf. This entry will be identical. | | `test_vcf` | The VCF file to use as benchmarking test input. The same file can be used in more than one row. File can be either vcf or vcf.gz. | | `caller` | Variant caller method used to generate test VCF file. There can be more than one test vcf for the same caller. For unknown caller use 'unknown' | -| `vartype` | Variant type to apply benchmarking. Variant type can be only one of these: small, sv, snv, indel and cnv. | An [example samplesheet](../assets/samplesheet.csv) has been provided with the pipeline. -## Defining Truth VCF and High confidence BED files - -The following parameters has to be defined for each type of benchmarking analysis. The following parameters defined the exact paths to the truth files: - -- `--sample`: Sample parameter defines the same name of the truth set. Examples: `HG002`, `SEQC2`, `HG001`, `HG003`, `CHM13`. -- `--analysis`: The type of analysis to perform: `germline` or `somatic`. -- `--method`: List the benchmarking methods to apply. By default all available tools will be applied according to the variant types provided. Available tools: `truvari, svanalyzer, happy, sompy, rtgtools, wittyer`. - -_Small variant benchmarking:_ - -- `--truth_small`: Path to the golden set VCF files combined for SNVs and indels, required for germline benchmarking (vcf or vcf.gz) -- `--high_conf_small`: Path to the high confidence BED files for SNVs and indels, required for germline benchmarking (bed or bed.gz) -- `--truth_snv`: Path to the golden set VCF files for SNVs, required for somatic benchmarking (vcf or vcf.gz) -- `--high_conf_snv`: Path to the high confidence BED files for SNVs, required for somatic benchmarking (bed or bed.gz) -- `--truth_indel`: Path to the golden set VCF files for indels, required for somatic benchmarking (vcf or vcf.gz) -- `--high_conf_indel`: Path to the high confidence BED files for indels, required for somatic benchmarking (bed or bed.gz) - -_Structural variant benchmarking:_ - -- `--truth_sv`: Path to the golden set VCF files for SVs, required for germline and somatic benchmarking (vcf or vcf.gz) -- `--high_conf_sv`: Path to the high confidence BED files for SVs, required for germline and somatic benchmarking (bed or bed.gz) - -_Copy Number Variation benchmarking:_ +## Truth samples -- `--truth_cnv`: Path to the golden set VCF files for CNVs, required for germline and somatic benchmarking (vcf or vcf.gz) -- `--high_conf_cnv`: Path to the high confidence BED files for CNVs, required for germline and somatic benchmarking (bed or bed.gz) - -_Using truth.config_ - -`conf/truth.config` file contains some readily available truth files for germline and somatic analysis. In order to activate usage one has to - -1. use `--genome` [`GRCh37` or `GRCh38`] -2. define `--sample` [`HG002` or `SEQC2`] -3. turn off `--itruth_ignore false` +Please find the detailed information about truth samples [here](https://nf-co.re/variantbenchmarking/truth). ## Lifting over truth sets @@ -78,8 +50,6 @@ This workflow comes with a liftover option for truth sets. In order to activate - `--chain`: This workflow uses picard tools for lifting over and a chain file has to be provided specific to the input truth vcf. Some examples can be found [here](https://genome.ucsc.edu/goldenPath/help/chain.html) - `--rename_chr`: Renaming chromosomes is required after liftover process. Some examples can be found under `assets/rename_contigs` directory. -Note: these two files are also provided under `itruth.config`. An example usage can be found in `conf/test_liftover.config` - ## Standardization and normalization parameters Consistent formatting and alignment of variants in test and truth VCF files for accurate comparison is controlled by _sv_standardization_ and _preprocesses_. @@ -100,10 +70,10 @@ Consistent formatting and alignment of variants in test and truth VCF files for If the input test vcf contains more than one sample, then user has to define which sample name to use. `subsample` will added to the samplesheet as an additional column as follows: ```csv title="samplesheet.csv" -id,test_vcf,caller,vartype,subsample -test1,test1.vcf.gz,delly,sv,"TUMOR" -test2,test2.vcf,gatk,small,"NA128120" -test3,test3.vcf.gz,cnvkit,cnv, +id,test_vcf,caller,subsample +test1,test1.vcf.gz,delly,"TUMOR" +test2,test2.vcf,gatk,"NA128120" +test3,test3.vcf.gz,cnvkit, ``` Note that, this option can be inevitable for somatic analysis since most of the callers reports both normal and tumor genotypes in the same vcf file. @@ -115,9 +85,9 @@ Benchmarking parameters may vary between the tools and for callers. In order to _SVbenchmark_ ```csv title="samplesheet.csv" -id,test_vcf,caller,vartype,normshift,normdist,normsizediff,maxdist -test1,test1.vcf.gz,delly,sv,0.7,0.7,0.7,100000 -test2,test2.vcf,gatk,sv,0.6,0.5,0.7,110000 +id,test_vcf,caller,normshift,normdist,normsizediff,maxdist +test1,test1.vcf.gz,delly,0.7,0.7,0.7,100000 +test2,test2.vcf,gatk,0.6,0.5,0.7,110000 ``` - `normshift`: Has to be between 0-1. Disallow matches if alignments between alternate alleles have normalized shift greater than normshift (default 0.2) @@ -128,9 +98,9 @@ test2,test2.vcf,gatk,sv,0.6,0.5,0.7,110000 _Truvari_ ```csv title="samplesheet.csv" -id,test_vcf,caller,vartype,pctsize,pctseq,pctovl,refdist,chunksize,dup_to_ins,typeignore -test1,test1.vcf.gz,delly,sv,0.7,0.7,0.7,100000,50000,true,true -test2,test2.vcf,gatk,sv,0.6,0.5,0.7,110000,40000,false,true +id,test_vcf,caller,pctsize,pctseq,pctovl,refdist,chunksize,dup_to_ins,typeignore +test1,test1.vcf.gz,delly,0.7,0.7,0.7,100000,50000,true,true +test2,test2.vcf,gatk,0.6,0.5,0.7,110000,40000,false,true ``` - `pctsize`: Has to be between 0-1. Ratio of min(base_size, comp_size)/max(base_size, comp_size) @@ -172,7 +142,7 @@ _Parameters applicable only to Structural Variants_ The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/variantbenchmarking --input ./samplesheet.csv --outdir ./results -profile docker --genome GRCh37 --sample HG002 --analysis germline +nextflow run nf-core/variantbenchmarking --input ./samplesheet.csv --outdir ./results -profile docker --genome GRCh37 --sample HG002 --analysis germline --variant_type small ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -261,14 +231,29 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - `test` - A profile with a complete configuration for automated testing - Includes links to test data so needs no other parameters -- `test_liftover` +- `test_full` + - A profile with a complete configuration for full size of sample testing + - Includes links to test data so needs no other parameters +- `liftover_hg37` - A profile with a complete configuration for using liftover of HG002 hg38 truth set to hg37 - Includes links to test data so needs no other parameters -- `test_germline` - - A profile with a complete configuration for a full test of HG002 sample from germline analysis +- `liftover_hg38` + - A profile with a complete configuration for using liftover of HG002 hg37 truth set to hg38 + - Includes links to test data so needs no other parameters +- `germline_small` + - A profile with a complete configuration for germline analysis with small variat type of data + - Includes links to test data so needs no other parameters +- `germline_structural` + - A profile with a complete configuration for germline analysis with structural variat type of data + - Includes links to test data so needs no other parameters +- `somatic_structural` + - A profile with a complete configuration for somatic analysis with structural variat type of data + - Includes links to test data so needs no other parameters +- `somatic_snv` + - A profile with a complete configuration for somatic analysis with snv variat type of data - Includes links to test data so needs no other parameters -- `test_somatic` - - A profile with a complete configuration for a full test of SEQC2 sample from somatic analysis +- `somatic_indel` + - A profile with a complete configuration for somatic analysis with indel variat type of data - Includes links to test data so needs no other parameters - `docker` - A generic configuration profile to be used with [Docker](https://docker.com/) diff --git a/nextflow.config b/nextflow.config index ce30578..c655a87 100644 --- a/nextflow.config +++ b/nextflow.config @@ -186,11 +186,15 @@ profiles { executor.cpus = 4 executor.memory = 8.GB } - test { includeConfig 'conf/test.config' } - test_stub { includeConfig 'conf/test_stub.config' } - test_full { includeConfig 'conf/test_full.config' } - test_somatic { includeConfig 'conf/test_somatic.config' } - test_liftover { includeConfig 'conf/test_liftover.config' } + test { includeConfig 'conf/test.config' } + test_full { includeConfig 'conf/test_full.config' } + germline_small { includeConfig 'conf/tests/germline_small.config'} + germline_sv { includeConfig 'conf/tests/germline_sv.config' } + somatic_snv { includeConfig 'conf/tests/somatic_snv.config' } + somatic_indel { includeConfig 'conf/tests/somatic_indel.config' } + somatic_sv { includeConfig 'conf/tests/somatic_sv.config' } + liftover_hg37 { includeConfig 'conf/tests/liftover_hg37.config' } + liftover_hg38 { includeConfig 'conf/tests/liftover_hg38.config' } } @@ -198,7 +202,6 @@ profiles { includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null" // Load nf-core/variantbenchmarking custom profiles from different institutions. -// TODO nf-core: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs // includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/variantbenchmarking.config" : "/dev/null" // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile From 23c173d897a05009466d40a15df33e4629388d15 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 20 Nov 2024 10:31:14 +0000 Subject: [PATCH 203/292] fix doc --- conf/tests/liftover_hg37.config | 2 +- conf/tests/liftover_hg38.config | 2 +- docs/output.md | 2 +- docs/usage.md | 2 +- 4 files changed, 4 insertions(+), 4 deletions(-) diff --git a/conf/tests/liftover_hg37.config b/conf/tests/liftover_hg37.config index ac9bc2a..e7d88bb 100644 --- a/conf/tests/liftover_hg37.config +++ b/conf/tests/liftover_hg37.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile liftover_hg37, --outdir + nextflow run nf-core/variantbenchmarking -profile liftover_hg37, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/conf/tests/liftover_hg38.config b/conf/tests/liftover_hg38.config index 561d19e..ae8a2ba 100644 --- a/conf/tests/liftover_hg38.config +++ b/conf/tests/liftover_hg38.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/benchmark -profile liftover_hg38, --outdir + nextflow run nf-core/variantbenchmarking -profile liftover_hg38, --outdir ---------------------------------------------------------------------------------------- */ diff --git a/docs/output.md b/docs/output.md index 66df667..8d8771c 100644 --- a/docs/output.md +++ b/docs/output.md @@ -19,7 +19,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Wittyer](#wittyer) - [RTG-tools](#rtgtools) - [Happy](#happy) - - [Sompy](#sompyh) + - [Sompy](#sompy) - [Summary statistics](#summary) - [Comparison of benchmarking results](#comparisons) - [Merged summary benchmark statistics](#tables) diff --git a/docs/usage.md b/docs/usage.md index 58451bc..b7c6047 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -22,7 +22,7 @@ You will need to create a samplesheet with information about the test vcf you wo ### Full samplesheet -The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 4 columns to match those defined in the table below. +The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 3 columns to match those defined in the table below. ```csv title="samplesheet.csv" id,test_vcf,caller From 92627c9dacc8fdf67c7ae09fc9a5065ca85b931b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 21 Nov 2024 12:20:57 +0000 Subject: [PATCH 204/292] adding plots to multiqc --- assets/multiqc_config.yml | 43 ++++++- bin/plots.R | 12 +- conf/modules.config | 6 +- modules.json | 2 +- modules/local/plots/main.nf | 4 +- modules/nf-core/multiqc/main.nf | 2 +- subworkflows/local/cnv_germline_benchmark.nf | 16 ++- .../local/compare_benchmark_results.nf | 5 +- subworkflows/local/liftover_vcfs_truth.nf | 5 +- subworkflows/local/prepare_vcfs_test.nf | 21 +--- subworkflows/local/prepare_vcfs_truth.nf | 10 +- .../local/report_benchmark_statistics.nf | 16 +-- subworkflows/local/report_vcf_statistics.nf | 24 ++-- .../local/small_germline_benchmark.nf | 28 ++--- subworkflows/local/small_somatic_benchmark.nf | 18 +-- .../local/split_small_variants_test.nf | 2 +- subworkflows/local/subsample_vcf_test.nf | 6 +- subworkflows/local/sv_germline_benchmark.nf | 36 +++--- subworkflows/local/sv_somatic_benchmark.nf | 4 +- subworkflows/local/sv_vcf_conversion.nf | 6 +- subworkflows/local/vcf_reheader_samplename.nf | 4 +- .../local/vcf_variant_deduplication.nf | 2 +- subworkflows/local/vcf_variant_filtering.nf | 4 +- workflows/variantbenchmarking.nf | 110 +++++++----------- 24 files changed, 190 insertions(+), 196 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 9b0ed73..1ec952a 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,5 +11,46 @@ report_section_order: order: -1002 export_plots: true - disable_version_detection: true +ignore_images: false + +# Run only these modules +run_modules: + - bcftools + +custom_data: + survivor: + id: "survivor" + section_name: "SURVIVOR variant statistics" + description: "generated by nf-core/survivor" + plot_type: "bargraph" + format: "tsv" + pconfig: + id: "survivor" + namespace: "SURVIVOR variant statistics" + table_title: "Variant statistics from survivor stats tool" + ylab: "Len" + headers: + - "Len" + - "Del" + - "INS" + - "Dup" + - "Inv" + - "TRA" + - "UNK" + + summary_reports: + id: "summary_reports" + section_name: "Summary Benchmark Reports" + +sp: + survivor: + fn: "*.stats" + summary_reports: + fn: "*.png" + +module_order: + - bcftools: + name: "Bcftools stats" + - survivor + - summary_reports diff --git a/bin/plots.R b/bin/plots.R index 0ad4d47..6734aef 100755 --- a/bin/plots.R +++ b/bin/plots.R @@ -13,21 +13,21 @@ generate_plots <- function(table, benchmark, type, filter, stats) { table = table[table$Type == type & table$Filter == filter, ] title1 = paste("Type=",type," Filter=",filter, " | TP/FP/FN by tool", sep="") title2 = paste("Type=",type," Filter=",filter, " | Precision, Recall, and F1 by Tool", sep="") - name1 = paste(type, "_", filter, "_metric_by_tool_", benchmark, ".png", sep = "") - name2 = paste(type, "_", filter, "_variants_by_tool_", benchmark, ".png", sep = "") + name1 = paste(type, "_", filter, "_metric_by_tool_", benchmark, "_mqc.png", sep = "") + name2 = paste(type, "_", filter, "_variants_by_tool_", benchmark, "_mqc.png", sep = "") } else if (stats != "None" ){ table = table[table$StatsType == stats, ] title1 = paste("StatsType=",stats, " | TP/FP/FN by tool", sep="") title2 = paste("StatsType=",stats, " | Precision, Recall, and F1 by Tool", sep="") - name1 = paste(stats, "_metric_by_tool_", benchmark, ".png", sep = "") - name2 = paste(stats, "_variants_by_tool_", benchmark, ".png", sep = "") + name1 = paste(stats, "_metric_by_tool_", benchmark, "_mqc.png", sep = "") + name2 = paste(stats, "_variants_by_tool_", benchmark, "_mqc.png", sep = "") } else{ title1 = paste("TP/FP/FN by tool", sep="") title2 = paste("Precision, Recall, and F1 by Tool", sep="") - name1 = paste("metric_by_tool_", benchmark, ".png", sep = "") - name2 = paste("variants_by_tool_", benchmark, ".png", sep = "") + name1 = paste("metric_by_tool_", benchmark, "_mqc.png", sep = "") + name2 = paste("variants_by_tool_", benchmark, "_mqc.png", sep = "") } input_data_melted <- melt(table, id.vars = "Tool") diff --git a/conf/modules.config b/conf/modules.config index dcce096..ea28690 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -136,7 +136,7 @@ process { // Variant stats withName: SURVIVOR_STATS { ext.prefix ={["${meta.id}", - (meta.caller != null) ? ".${meta.caller}" : '' + (meta.caller != null) ? ".${meta.caller}_mqc" : '_mqc' ].join('').trim() } publishDir = [ @@ -243,7 +243,7 @@ process { (meta.maxMatches != null) ? "--mm ${meta.maxMatches}" : '' ].join(' ').trim()} publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/wittyer_bench"}, + path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/wittyer"}, pattern: "*{.vcf.gz,tbi,json}", mode: params.publish_dir_mode ] @@ -266,7 +266,7 @@ process { ] } withName: PLOTS { - ext.prefix = {"${meta.benchmark_tool}"} + ext.prefix = {"${meta.benchmark_tool}_mqc"} publishDir = [ path: {"${params.outdir}/${params.variant_type}/summary/plots/${meta.benchmark_tool}"}, pattern: "*{png}", diff --git a/modules.json b/modules.json index cfd26cd..24d7a0a 100644 --- a/modules.json +++ b/modules.json @@ -82,7 +82,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "b8d36829fa84b6e404364abff787e8b07f6d058c", + "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d", "installed_by": ["modules"] }, "picard/createsequencedictionary": { diff --git a/modules/local/plots/main.nf b/modules/local/plots/main.nf index ed8da3a..f5f3a66 100644 --- a/modules/local/plots/main.nf +++ b/modules/local/plots/main.nf @@ -11,8 +11,8 @@ process PLOTS { tuple val(meta), path(summary) output: - tuple val(meta),path("*.png"), emit: plots - path "versions.yml" , emit: versions + path("*.png") , emit: plots + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 9724d2f..cc0643e 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -52,7 +52,7 @@ process MULTIQC { stub: """ mkdir multiqc_data - touch multiqc_plots + mkdir multiqc_plots touch multiqc_report.html cat <<-END_VERSIONS > versions.yml diff --git a/subworkflows/local/cnv_germline_benchmark.nf b/subworkflows/local/cnv_germline_benchmark.nf index a69af28..c1e631d 100644 --- a/subworkflows/local/cnv_germline_benchmark.nf +++ b/subworkflows/local/cnv_germline_benchmark.nf @@ -9,32 +9,30 @@ include { TABIX_BGZIP as TABIX_BGZIP_TRUTH } from '../../modules/nf-core/tabix/b workflow CNV_GERMLINE_BENCHMARK { take: input_ch // channel: [val(meta),test_vcf,test_index,truth_vcf,truth_index, bed] - fasta // reference channel [val(meta), ref.fa] - fai // reference channel [val(meta), ref.fa.fai] main: - versions = Channel.empty() + versions = Channel.empty() summary_reports = Channel.empty() // CNV benchmarking is only possible with wittyer now! TABIX_BGZIP_QUERY( - input_ch.map{ meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map{ meta, vcf, _tbi, _truth_vcf, _truth_tbi, _bed -> [ meta, vcf ] } ) versions = versions.mix(TABIX_BGZIP_QUERY.out.versions.first()) TABIX_BGZIP_TRUTH( - input_ch.map{ meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map{ meta, _vcf, _tbi, truth_vcf, _truth_tbi, _bed -> [ meta, truth_vcf ] } ) versions = versions.mix(TABIX_BGZIP_TRUTH.out.versions.first()) - input_ch.map{ meta, vcf, tbi, truth_vcf, truth_tbi, bed -> - [ meta, bed ] + input_ch.map{ meta, _vcf, _tbi, _truth_vcf, _truth_tbi, bedfile -> + [ meta, bedfile ] } .set { bed } @@ -56,6 +54,6 @@ workflow CNV_GERMLINE_BENCHMARK { emit: - summary_reports - versions + summary_reports // channel: [val(meta), summary] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/compare_benchmark_results.nf b/subworkflows/local/compare_benchmark_results.nf index d95f15a..22fed95 100644 --- a/subworkflows/local/compare_benchmark_results.nf +++ b/subworkflows/local/compare_benchmark_results.nf @@ -63,7 +63,6 @@ workflow COMPARE_BENCHMARK_RESULTS { merged_vcfs = merged_vcfs.mix(SURVIVOR_MERGE.out.vcf) } - // convert vcf files to csv VCF_TO_CSV( merged_vcfs @@ -71,6 +70,6 @@ workflow COMPARE_BENCHMARK_RESULTS { versions = versions.mix(VCF_TO_CSV.out.versions.first()) emit: - versions - merged_vcfs + merged_vcfs // channel: [val(meta), vcf] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/liftover_vcfs_truth.nf b/subworkflows/local/liftover_vcfs_truth.nf index b02cb5c..c52a1e6 100644 --- a/subworkflows/local/liftover_vcfs_truth.nf +++ b/subworkflows/local/liftover_vcfs_truth.nf @@ -59,7 +59,7 @@ workflow LIFTOVER_VCFS_TRUTH { // liftover high confidence file if given UCSC_LIFTOVER( high_conf_ch.map{file -> tuple([id: params.truth_id], file)}, - chain.map{meta, file -> file} + chain.map{_meta, file -> file} ) versions = versions.mix(UCSC_LIFTOVER.out.versions.first()) @@ -73,11 +73,10 @@ workflow LIFTOVER_VCFS_TRUTH { SORT_BED.out.bed ) versions = versions.mix(BEDTOOLS_MERGE.out.versions.first()) - bed_ch = BEDTOOLS_MERGE.out.bed emit: vcf_ch // channel: [val(meta), vcf.gz] bed_ch // channel: [val(meta), bed] - versions + versions // channel: [versions.yml] } diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index e1a6f3f..fdf2c32 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -87,29 +87,18 @@ workflow PREPARE_VCFS_TEST { if (params.analysis.contains("somatic")){ - // somatic spesific preperations - //vcf_ch.branch{ - // def meta = it[0] - // small: meta.vartype == "small" - // other: true - // } - // .set{vcf} - + // somatic specific preparations if (params.variant_type == "small"){ - out_vcf_ch = Channel.empty() - SPLIT_SMALL_VARIANTS_TEST( - vcf.small + vcf_ch ) versions = versions.mix(SPLIT_SMALL_VARIANTS_TEST.out.versions.first()) - out_vcf_ch = out_vcf_ch.mix(SPLIT_SMALL_VARIANTS_TEST.out.out_vcf_ch, - vcf.other) - vcf_ch = out_vcf_ch + vcf_ch = SPLIT_SMALL_VARIANTS_TEST.out.out_vcf_ch } } emit: - vcf_ch - versions + vcf_ch // channel: [val(meta), vcf.gz, tbi] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 4db015f..1f6b372 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -37,7 +37,7 @@ workflow PREPARE_VCFS_TRUTH { ) versions = versions.mix(LIFTOVER_VCFS_TRUTH.out.versions.first()) truth_ch = LIFTOVER_VCFS_TRUTH.out.vcf_ch - high_conf_ch = LIFTOVER_VCFS_TRUTH.out.bed_ch.map{ meta, bed -> [bed]} + high_conf_ch = LIFTOVER_VCFS_TRUTH.out.bed_ch.map{ _meta, bed -> [bed]} } // Reheader sample name for truth file - using meta.caller @@ -50,7 +50,7 @@ workflow PREPARE_VCFS_TRUTH { if (params.preprocess.contains("normalization")){ - // multi-allelic variants will be splitted. + // multi-allelic variants will be splitter. BCFTOOLS_NORM( vcf_ch, fasta @@ -78,7 +78,7 @@ workflow PREPARE_VCFS_TRUTH { } emit: - vcf_ch - high_conf_ch - versions + vcf_ch // channel: [val(meta), vcf, tbi] + high_conf_ch // channel: [val(meta), bed] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/report_benchmark_statistics.nf b/subworkflows/local/report_benchmark_statistics.nf index a58afed..cb3cad5 100644 --- a/subworkflows/local/report_benchmark_statistics.nf +++ b/subworkflows/local/report_benchmark_statistics.nf @@ -4,8 +4,8 @@ include { MERGE_REPORTS } from '../../modules/local/merge_reports' include { PLOTS } from '../../modules/local/plots' -include { CREATE_DATAVZRD_INPUT } from '../../modules/local/create_datavzrd_input' addParams( options: params.options ) -include { DATAVZRD } from '../../modules/nf-core/datavzrd' addParams( options: params.options ) +include { CREATE_DATAVZRD_INPUT } from '../../modules/local/create_datavzrd_input' +include { DATAVZRD } from '../../modules/nf-core/datavzrd' workflow REPORT_BENCHMARK_STATISTICS { take: @@ -14,6 +14,8 @@ workflow REPORT_BENCHMARK_STATISTICS { main: versions = Channel.empty() + ch_plots = Channel.empty() + // merge summary statistics from the same benchmarking tool MERGE_REPORTS( reports @@ -24,6 +26,7 @@ workflow REPORT_BENCHMARK_STATISTICS { PLOTS( MERGE_REPORTS.out.summary ) + ch_plots = ch_plots.mix(PLOTS.out.plots.flatten()) versions = versions.mix(PLOTS.out.versions.first()) MERGE_REPORTS.out.summary @@ -32,8 +35,8 @@ workflow REPORT_BENCHMARK_STATISTICS { // add path to csv file to the datavzrd input summary - .map { meta, summary -> - [ meta, summary, file("${projectDir}/assets/datavzrd/${meta.id}.datavzrd.template.yaml", checkIfExists:true) ] + .map { meta, summary_file -> + [ meta, summary_file, file("${projectDir}/assets/datavzrd/${meta.id}.datavzrd.template.yaml", checkIfExists:true) ] } .set {template} @@ -48,8 +51,7 @@ workflow REPORT_BENCHMARK_STATISTICS { ) versions = versions.mix(DATAVZRD.out.versions.first()) - datavzrd_report = DATAVZRD.out.report - emit: - versions + versions // channel: [versions.yml] + ch_plots // channel: [plots.png] } diff --git a/subworkflows/local/report_vcf_statistics.nf b/subworkflows/local/report_vcf_statistics.nf index b6c283a..dc89afc 100644 --- a/subworkflows/local/report_vcf_statistics.nf +++ b/subworkflows/local/report_vcf_statistics.nf @@ -11,27 +11,20 @@ workflow REPORT_VCF_STATISTICS { main: - versions = Channel.empty() - survivor_stats = Channel.empty() - - //input_ch.branch{ - // def meta = it[0] - // sv: meta.vartype == "sv" || meta.vartype == "cnv" - // other: true - // } - // .set{input} + versions = Channel.empty() + ch_stats = Channel.empty() if (params.variant_type == "structural"){ // use survivor stats to get SV statistics by TYPE SURVIVOR_STATS( - input_ch.map{ meta, vcf, tbi -> + input_ch.map{ meta, vcf, _tbi -> [ meta, vcf ] }, -1, -1, -1 ) - survivor_stats = SURVIVOR_STATS.out.stats + ch_stats = ch_stats.mix(SURVIVOR_STATS.out.stats.map{_meta, stats -> stats}) versions = versions.mix(SURVIVOR_STATS.out.versions.first()) } @@ -45,13 +38,12 @@ workflow REPORT_VCF_STATISTICS { [[],[]], [[],[]] ) - bcftools_stats = BCFTOOLS_STATS.out.stats + ch_stats = ch_stats.mix(BCFTOOLS_STATS.out.stats.map{_meta, stats -> stats}) versions = versions.mix(BCFTOOLS_STATS.out.versions.first()) - // Add here a tool, to visualize SV statistics in a histogram. + // TODO: Add here a tool, to visualize SV statistics in a histogram. emit: - bcftools_stats - survivor_stats - versions + ch_stats // channel: [stats] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/small_germline_benchmark.nf b/subworkflows/local/small_germline_benchmark.nf index a74ee9c..5e07217 100644 --- a/subworkflows/local/small_germline_benchmark.nf +++ b/subworkflows/local/small_germline_benchmark.nf @@ -30,7 +30,7 @@ workflow SMALL_GERMLINE_BENCHMARK { // Use rtgtools format to generate sdf file if necessary RTGTOOLS_FORMAT( - fasta.map { meta, fasta -> [ meta, fasta, [], [] ] } + fasta.map { meta, file -> [ meta, file, [], [] ] } ) versions = versions.mix(RTGTOOLS_FORMAT.out.versions) sdf = RTGTOOLS_FORMAT.out.sdf @@ -48,13 +48,13 @@ workflow SMALL_GERMLINE_BENCHMARK { // collect summary reports RTGTOOLS_VCFEVAL.out.summary - .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "rtgtools"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "rtgtools"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) - // reheader benchmarking results preperly and tag meta + // reheader benchmarking results properly and tag meta VCF_REHEADER_SAMPLENAME_1( RTGTOOLS_VCFEVAL.out.fn_vcf, fai @@ -62,7 +62,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_1.out.versions) VCF_REHEADER_SAMPLENAME_1.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "rtgtools"], file, index) } + .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "rtgtools"], file, index) } .set { vcf_fn } VCF_REHEADER_SAMPLENAME_2( @@ -72,7 +72,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_2.out.versions) VCF_REHEADER_SAMPLENAME_2.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "rtgtools"], file, index) } + .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "rtgtools"], file, index) } .set { vcf_fp } VCF_REHEADER_SAMPLENAME_3( @@ -82,7 +82,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_3.out.versions) VCF_REHEADER_SAMPLENAME_3.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "rtgtools"], file, index) } + .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "rtgtools"], file, index) } .set { vcf_tp_base } VCF_REHEADER_SAMPLENAME_4( @@ -92,7 +92,7 @@ workflow SMALL_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_4.out.versions) VCF_REHEADER_SAMPLENAME_4.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "rtgtools"], file, index) } + .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "rtgtools"], file, index) } .set { vcf_tp_comp } tagged_variants = tagged_variants.mix( @@ -106,13 +106,13 @@ workflow SMALL_GERMLINE_BENCHMARK { if (params.method.contains('happy')){ input_ch - .map{ meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + .map{ meta, vcf, _tbi, _truth_vcf, _truth_tbi, _bed -> [ meta, vcf ] } .set { test_ch } input_ch - .map{ meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + .map{ meta, _vcf, _tbi, truth_vcf, _truth_tbi, bed -> [ meta, truth_vcf, bed, [] ] } .set { truth_ch } @@ -121,7 +121,7 @@ workflow SMALL_GERMLINE_BENCHMARK { // apply prepy if required HAPPY_PREPY( - input_ch.map{ meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map{ meta, vcf, _tbi, _truth_vcf, _truth_tbi, bed -> [ meta, vcf, bed ] }, fasta, @@ -146,14 +146,14 @@ workflow SMALL_GERMLINE_BENCHMARK { // tag meta and collect summary reports HAPPY_HAPPY.out.summary_csv - .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "happy"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "happy"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) } emit: - versions - summary_reports - tagged_variants + summary_reports // channel: [val(meta), reports] + tagged_variants // channel: [val(meta), vcfs] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/small_somatic_benchmark.nf b/subworkflows/local/small_somatic_benchmark.nf index 78fa535..27e36cc 100644 --- a/subworkflows/local/small_somatic_benchmark.nf +++ b/subworkflows/local/small_somatic_benchmark.nf @@ -13,13 +13,13 @@ workflow SMALL_SOMATIC_BENCHMARK { main: - versions = Channel.empty() - summary_reports = Channel.empty() + versions = Channel.empty() + summary_reports = Channel.empty() if (params.method.contains('sompy')){ - // apply sompy for small somatic variant ebnchmarking + // apply sompy for small somatic variant benchmarking HAPPY_SOMPY( - input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map { meta, vcf, _tbi, truth_vcf, _truth_tbi, bed -> [ meta, vcf, truth_vcf, bed, [] ] }, fasta, @@ -31,7 +31,7 @@ workflow SMALL_SOMATIC_BENCHMARK { versions = versions.mix(HAPPY_SOMPY.out.versions.first()) HAPPY_SOMPY.out.stats - .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "sompy"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "sompy"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) @@ -41,7 +41,7 @@ workflow SMALL_SOMATIC_BENCHMARK { if (params.method.contains('bamsurgeon')){ BAMSURGEON_EVALUATOR( - input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, _bed -> [ meta, vcf, tbi, truth_vcf, truth_tbi ] }, fasta, @@ -50,13 +50,13 @@ workflow SMALL_SOMATIC_BENCHMARK { versions = versions.mix(BAMSURGEON_EVALUATOR.out.versions.first()) BAMSURGEON_EVALUATOR.out.stats - .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "bamsurgeon"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "bamsurgeon"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) } emit: - summary_reports - versions + summary_reports // channel: [val(meta), reports] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/split_small_variants_test.nf b/subworkflows/local/split_small_variants_test.nf index 3f00511..00f26fd 100644 --- a/subworkflows/local/split_small_variants_test.nf +++ b/subworkflows/local/split_small_variants_test.nf @@ -54,5 +54,5 @@ workflow SPLIT_SMALL_VARIANTS_TEST { emit: out_vcf_ch // channel: [val(meta), vcf, index] - versions + versions // channel: [versions.yml] } diff --git a/subworkflows/local/subsample_vcf_test.nf b/subworkflows/local/subsample_vcf_test.nf index 6e34379..958b891 100644 --- a/subworkflows/local/subsample_vcf_test.nf +++ b/subworkflows/local/subsample_vcf_test.nf @@ -21,7 +21,7 @@ workflow SUBSAMPLE_VCF_TEST { ) versions = versions.mix(BCFTOOLS_SORT.out.versions) - // Subsamples sample name for multisample vcfs + // Subsample sample name for multisample vcfs BCFTOOLS_VIEW_SUBSAMPLE( BCFTOOLS_SORT.out.vcf.map{ meta, vcf -> tuple(meta, vcf, []) }, [], @@ -30,7 +30,7 @@ workflow SUBSAMPLE_VCF_TEST { ) versions = versions.mix(BCFTOOLS_VIEW_SUBSAMPLE.out.versions.first()) - // filters out ./. genotypes (remainings from multisample vcf) + // filters out ./. genotypes (remaining from multisample vcf) BCFTOOLS_VIEW_FILTERMISSING( BCFTOOLS_VIEW_SUBSAMPLE.out.vcf.map{ meta, vcf -> tuple(meta, vcf, []) }, [], @@ -43,5 +43,5 @@ workflow SUBSAMPLE_VCF_TEST { emit: vcf_ch // channel: [val(meta), vcf] - versions + versions // channel: [versions.yml] } diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index 56fa2a3..780265c 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -20,9 +20,9 @@ workflow SV_GERMLINE_BENCHMARK { main: - versions = Channel.empty() - summary_reports = Channel.empty() - tagged_variants = Channel.empty() + versions = Channel.empty() + summary_reports = Channel.empty() + tagged_variants = Channel.empty() // SV benchmarking if (params.method.contains('truvari')){ @@ -36,7 +36,7 @@ workflow SV_GERMLINE_BENCHMARK { versions = versions.mix(TRUVARI_BENCH.out.versions.first()) TRUVARI_BENCH.out.summary - .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "truvari"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "truvari"], file) } .groupTuple() .set { report } @@ -50,7 +50,7 @@ workflow SV_GERMLINE_BENCHMARK { versions = versions.mix(VCF_REHEADER_SAMPLENAME_1.out.versions) VCF_REHEADER_SAMPLENAME_1.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "truvari"], file) } + .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "truvari"], file) } .set { vcf_fn } // reheader fp vcf files for tagged results @@ -62,7 +62,7 @@ workflow SV_GERMLINE_BENCHMARK { // add tag and to meta VCF_REHEADER_SAMPLENAME_2.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "truvari"], file) } + .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "truvari"], file) } .set { vcf_fp } // reheader base tp vcf files for tagged results @@ -74,7 +74,7 @@ workflow SV_GERMLINE_BENCHMARK { // add tag and to meta VCF_REHEADER_SAMPLENAME_3.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "truvari"], file) } + .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "truvari"], file) } .set { vcf_tp_base } // reheader comp tp vcf files for tagged results @@ -86,7 +86,7 @@ workflow SV_GERMLINE_BENCHMARK { // add tag and to meta VCF_REHEADER_SAMPLENAME_4.out.ch_vcf - .map { meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "truvari"], file) } + .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "truvari"], file) } .set { vcf_tp_comp } // collect tagged variant files @@ -108,7 +108,7 @@ workflow SV_GERMLINE_BENCHMARK { // tag and collect summary file SVANALYZER_SVBENCHMARK.out.report - .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "svbenchmark"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "svbenchmark"], file) } .groupTuple() .set{ report } @@ -116,12 +116,12 @@ workflow SV_GERMLINE_BENCHMARK { // reheader fn vcf files for tagged results SVANALYZER_SVBENCHMARK.out.fns - .map { meta, file -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "svbenchmark"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "svbenchmark"], file) } .set { vcf_fn } SVANALYZER_SVBENCHMARK.out.fps - .map { meta, file -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "svbenchmark"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "svbenchmark"], file) } .set { vcf_fp } tagged_variants = tagged_variants.mix( @@ -134,20 +134,20 @@ workflow SV_GERMLINE_BENCHMARK { // unzip vcf.gz files TABIX_BGZIP_QUERY( - input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map { meta, vcf, _tbi, _truth_vcf, _truth_tbi, _bed -> [ meta, vcf ] } ) versions = versions.mix(TABIX_BGZIP_QUERY.out.versions.first()) TABIX_BGZIP_TRUTH( - input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map { meta, _vcf, _tbi, truth_vcf, _truth_tbi, _bed -> [ meta, truth_vcf ] } ) versions = versions.mix(TABIX_BGZIP_TRUTH.out.versions.first()) - input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, bed -> + input_ch.map { meta, _vcf, _tbi, _truth_vcf, _truth_tbi, bed -> [ meta, bed ] } .set { bed } @@ -163,7 +163,7 @@ workflow SV_GERMLINE_BENCHMARK { versions = versions.mix(WITTYER.out.versions.first()) WITTYER.out.report - .map { meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "wittyer"], file) } + .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "wittyer"], file) } .groupTuple() .set{ report } summary_reports = summary_reports.mix(report) @@ -171,7 +171,7 @@ workflow SV_GERMLINE_BENCHMARK { } emit: - tagged_variants - summary_reports - versions + tagged_variants // channel: [val(meta), vcfs] + summary_reports // channel: [val(meta), reports] + versions // channel: [val(meta), versions.yml] } diff --git a/subworkflows/local/sv_somatic_benchmark.nf b/subworkflows/local/sv_somatic_benchmark.nf index b9b31ab..015f69b 100644 --- a/subworkflows/local/sv_somatic_benchmark.nf +++ b/subworkflows/local/sv_somatic_benchmark.nf @@ -35,6 +35,6 @@ workflow SV_SOMATIC_BENCHMARK { } emit: - summary_reports - versions + summary_reports // channel: [val(meta), reports] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index c92e214..92e08cd 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -1,7 +1,7 @@ import groovy.io.FileType // -// SV_VCF_CONVERSIONS: SUBWORKFLOW TO apply tool spesific conversions +// SV_VCF_CONVERSIONS: SUBWORKFLOW to apply tool spesific conversions // include { SVYNC } from '../../modules/nf-core/svync' @@ -86,6 +86,6 @@ workflow SV_VCF_CONVERSIONS { } emit: - vcf_ch - versions + vcf_ch // channel: [val(meta), vcf] + versions // channel: [versions.yml] } diff --git a/subworkflows/local/vcf_reheader_samplename.nf b/subworkflows/local/vcf_reheader_samplename.nf index c28c022..5d4384d 100644 --- a/subworkflows/local/vcf_reheader_samplename.nf +++ b/subworkflows/local/vcf_reheader_samplename.nf @@ -35,7 +35,7 @@ workflow VCF_REHEADER_SAMPLENAME { emit: - ch_vcf // channel: [ val(meta), vcf, index ] - versions // channel: [ versions.yml ] + ch_vcf // channel: [val(meta), vcf, index ] + versions // channel: [versions.yml ] } diff --git a/subworkflows/local/vcf_variant_deduplication.nf b/subworkflows/local/vcf_variant_deduplication.nf index dffba56..81b7496 100644 --- a/subworkflows/local/vcf_variant_deduplication.nf +++ b/subworkflows/local/vcf_variant_deduplication.nf @@ -40,6 +40,6 @@ workflow VCF_VARIANT_DEDUPLICATION { emit: ch_vcf // channel: [ val(meta), vcf,index ] - versions // channel: [ versions.yml ] + versions // channel: [versions.yml ] } diff --git a/subworkflows/local/vcf_variant_filtering.nf b/subworkflows/local/vcf_variant_filtering.nf index fda853a..7f4271f 100644 --- a/subworkflows/local/vcf_variant_filtering.nf +++ b/subworkflows/local/vcf_variant_filtering.nf @@ -56,6 +56,6 @@ workflow VCF_VARIANT_FILTERING { vcf_ch = TABIX_BGZIPTABIX.out.gz_tbi emit: - vcf_ch // [val(meta), vcf.gz, index] - versions + vcf_ch // [val(meta), vcf.gz, index] + versions // channel: [versions.yml] } diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 734bb00..9cec93b 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -1,14 +1,3 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS @@ -18,11 +7,11 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // MODULE: Installed directly from nf-core/modules // -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { paramsSummaryMap } from 'plugin/nf-schema' -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-schema' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_variantbenchmarking_pipeline' // // SUBWORKFLOWS: Local Subworkflows @@ -51,8 +40,11 @@ workflow VARIANTBENCHMARKING { ch_samplesheet // channel: samplesheet read in from --input main: + // To gather all QC reports for Multiqc ch_versions = Channel.empty() ch_multiqc_files = Channel.empty() + + // To gather benchmark reports ch_reports = Channel.empty() //// create reference channels //// @@ -74,7 +66,7 @@ workflow VARIANTBENCHMARKING { sdf = params.sdf ? Channel.fromPath(params.sdf, checkIfExists: true).map{ sdf -> tuple([id: sdf.getSimpleName()], sdf) }.collect() : Channel.empty() - // read chainfile, liftover genome and rename chr files if liftover is true + // read chain file, liftover genome and rename chr files if liftover is true chain = Channel.empty() rename_chr = Channel.empty() dictionary = Channel.empty() @@ -110,14 +102,14 @@ workflow VARIANTBENCHMARKING { if (params.variant_type == "structural"){ - // Standardize SV VCFs, tool spesific modifications + // Standardize SV VCFs, tool specific modifications SV_VCF_CONVERSIONS( vcf_ch, fasta, fai ) ch_versions = ch_versions.mix(SV_VCF_CONVERSIONS.out.versions) - vcf_ch = SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])} + vcf_ch = SV_VCF_CONVERSIONS.out.vcf_ch.map{it -> tuple(it[0], it[1])} } // Prepare and normalize input vcfs PREPARE_VCFS_TEST( @@ -138,7 +130,7 @@ workflow VARIANTBENCHMARKING { dictionary ) regions_bed_ch = PREPARE_VCFS_TRUTH.out.high_conf_ch - ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) + ch_versions = ch_versions.mix(PREPARE_VCFS_TRUTH.out.versions) // VCF REPORTS AND STATS @@ -146,12 +138,13 @@ workflow VARIANTBENCHMARKING { REPORT_VCF_STATISTICS( PREPARE_VCFS_TEST.out.vcf_ch.mix(PREPARE_VCFS_TRUTH.out.vcf_ch) ) - ch_versions = ch_versions.mix(REPORT_VCF_STATISTICS.out.versions) + ch_versions = ch_versions.mix(REPORT_VCF_STATISTICS.out.versions) + ch_multiqc_files = ch_multiqc_files.mix(REPORT_VCF_STATISTICS.out.ch_stats) // Prepare benchmark channel PREPARE_VCFS_TEST.out.vcf_ch.combine(PREPARE_VCFS_TRUTH.out.vcf_ch) .combine(regions_bed_ch.ifEmpty([[]])) - .map{ test_meta, test_vcf, test_tbi, truth_meta, truth_vcf, truth_tbi, high_bed -> + .map{ test_meta, test_vcf, test_tbi, _truth_meta, truth_vcf, truth_tbi, high_bed -> [ test_meta, test_vcf, test_tbi, truth_vcf, truth_tbi, high_bed ]} .set{bench} @@ -165,35 +158,33 @@ workflow VARIANTBENCHMARKING { fasta, fai ) - ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(SV_GERMLINE_BENCHMARK.out.summary_reports) - evals_ch = evals_ch.mix(SV_GERMLINE_BENCHMARK.out.tagged_variants) + ch_versions = ch_versions.mix(SV_GERMLINE_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(SV_GERMLINE_BENCHMARK.out.summary_reports) + evals_ch = evals_ch.mix(SV_GERMLINE_BENCHMARK.out.tagged_variants) } if (params.analysis.contains("germline")){ if (params.variant_type == "small" | params.variant_type == "snv" | params.variant_type == "indel"){ - // Benchmarking spesific to small germline samples + // Benchmarking specific to small germline samples SMALL_GERMLINE_BENCHMARK( bench, fasta, fai, sdf ) - ch_versions = ch_versions.mix(SMALL_GERMLINE_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(SMALL_GERMLINE_BENCHMARK.out.summary_reports) - evals_ch = evals_ch.mix(SMALL_GERMLINE_BENCHMARK.out.tagged_variants) + ch_versions = ch_versions.mix(SMALL_GERMLINE_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(SMALL_GERMLINE_BENCHMARK.out.summary_reports) + evals_ch = evals_ch.mix(SMALL_GERMLINE_BENCHMARK.out.tagged_variants) } if (params.variant_type == "copynumber"){ // Benchmarking spesific to CNV germline samples CNV_GERMLINE_BENCHMARK( - bench, - fasta, - fai + bench ) - ch_versions = ch_versions.mix(CNV_GERMLINE_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(CNV_GERMLINE_BENCHMARK.out.summary_reports) + ch_versions = ch_versions.mix(CNV_GERMLINE_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(CNV_GERMLINE_BENCHMARK.out.summary_reports) } } @@ -207,8 +198,8 @@ workflow VARIANTBENCHMARKING { fasta, fai ) - ch_versions = ch_versions.mix(SMALL_SOMATIC_BENCHMARK.out.versions) - ch_reports = ch_reports.mix(SMALL_SOMATIC_BENCHMARK.out.summary_reports) + ch_versions = ch_versions.mix(SMALL_SOMATIC_BENCHMARK.out.versions) + ch_reports = ch_reports.mix(SMALL_SOMATIC_BENCHMARK.out.summary_reports) } } @@ -224,7 +215,7 @@ workflow VARIANTBENCHMARKING { REPORT_BENCHMARK_STATISTICS( ch_reports ) - ch_versions = ch_versions.mix(REPORT_BENCHMARK_STATISTICS.out.versions) + ch_versions = ch_versions.mix(REPORT_BENCHMARK_STATISTICS.out.versions) // TODO: BENCHMARKING OF CNV // https://bioconductor.org/packages/release/bioc/manuals/CNVfilteR/man/CNVfilteR.pdf @@ -251,36 +242,19 @@ workflow VARIANTBENCHMARKING { // // MODULE: MultiQC // - ch_multiqc_config = Channel.fromPath( - "$projectDir/assets/multiqc_config.yml", checkIfExists: true) - ch_multiqc_custom_config = params.multiqc_config ? - Channel.fromPath(params.multiqc_config, checkIfExists: true) : - Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? - Channel.fromPath(params.multiqc_logo, checkIfExists: true) : - Channel.empty() - - - summary_params = paramsSummaryMap( - workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - ch_multiqc_files = ch_multiqc_files.mix( - ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? - file(params.multiqc_methods_description, checkIfExists: true) : - file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value( - methodsDescriptionText(ch_multiqc_custom_methods_description)) - - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix( - ch_methods_description.collectFile( - name: 'methods_description_mqc.yaml', - sort: true - ) - ) - + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) :Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml',sort: true)) + ch_multiqc_files = ch_multiqc_files.mix(REPORT_BENCHMARK_STATISTICS.out.ch_plots) + + ch_multiqc_files.view() MULTIQC ( ch_multiqc_files.collect(), ch_multiqc_config.toList(), @@ -291,7 +265,7 @@ workflow VARIANTBENCHMARKING { ) emit:multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html - versions = ch_versions // channel: [ path(versions.yml) ] + versions = ch_versions // channel: [ path(versions.yml) ] } From 59a8a10ce0f8aa324da1bb7ac3629d45175a382c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 22 Nov 2024 10:43:11 +0000 Subject: [PATCH 205/292] renew snapshots --- conf/tests/germline_sv.config | 2 +- tests/germline_small.nf.test.snap | 103 +++++++++++---- tests/germline_sv.nf.test.snap | 212 ++++++++++++++++++------------ tests/liftover_hg38.nf.test.snap | 77 ++++++++--- tests/somatic_indel.nf.test.snap | 48 ++++++- tests/somatic_snv.nf.test.snap | 110 +--------------- tests/somatic_sv.nf.test.snap | 62 ++++++--- 7 files changed, 361 insertions(+), 253 deletions(-) diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config index f6b60b7..ddd89b4 100644 --- a/conf/tests/germline_sv.config +++ b/conf/tests/germline_sv.config @@ -33,7 +33,7 @@ params { analysis = 'germline' variant_type = "structural" method = 'svanalyzer,wittyer,truvari' - preprocess = "normalization,deduplication" + preprocess = "normalization,deduplication,filter_contigs" sv_standardization = "svync,homogenize" min_sv_size = 30 truth_id = "HG002" diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 500de1a..7fe0713 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -77,11 +77,10 @@ "small/multiqc/multiqc_report.html", "small/summary", "small/summary/comparisons", - "small/summary/comparisons/small", - "small/summary/comparisons/small/rtgtools.FN.csv", - "small/summary/comparisons/small/rtgtools.FP.csv", - "small/summary/comparisons/small/rtgtools.TP_base.csv", - "small/summary/comparisons/small/rtgtools.TP_comp.csv", + "small/summary/comparisons/rtgtools.FN.csv", + "small/summary/comparisons/rtgtools.FP.csv", + "small/summary/comparisons/rtgtools.TP_base.csv", + "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", "small/summary/datavzrd/happy", "small/summary/datavzrd/happy/index.html", @@ -122,11 +121,11 @@ "small/summary/datavzrd/versions.yml", "small/summary/plots", "small/summary/plots/happy", - "small/summary/plots/happy/metric_by_tool_happy.png", - "small/summary/plots/happy/variants_by_tool_happy.png", + "small/summary/plots/happy/metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/variants_by_tool_happy_mqc.png", "small/summary/plots/rtgtools", - "small/summary/plots/rtgtools/metric_by_tool_rtgtools.png", - "small/summary/plots/rtgtools/variants_by_tool_rtgtools.png", + "small/summary/plots/rtgtools/metric_by_tool_rtgtools_mqc.png", + "small/summary/plots/rtgtools/variants_by_tool_rtgtools_mqc.png", "small/summary/tables", "small/summary/tables/happy", "small/summary/tables/happy/happy.regions.csv", @@ -206,7 +205,10 @@ [ "genome.sdf:md5,d41d8cd98f00b204e9800998ecf8427e", "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -243,9 +245,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:10:22.614364432" + "timestamp": "2024-11-22T10:13:33.920466332" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -342,19 +344,58 @@ "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", "small/multiqc", "small/multiqc/multiqc_data", + "small/multiqc/multiqc_data/bcftools-stats-subtypes.txt", + "small/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "small/multiqc/multiqc_data/bcftools_stats_variant_depths.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", "small/multiqc/multiqc_data/multiqc.log", + "small/multiqc/multiqc_data/multiqc_bcftools_stats.txt", "small/multiqc/multiqc_data/multiqc_citations.txt", "small/multiqc/multiqc_data/multiqc_data.json", + "small/multiqc/multiqc_data/multiqc_general_stats.txt", "small/multiqc/multiqc_data/multiqc_software_versions.txt", "small/multiqc/multiqc_data/multiqc_sources.txt", + "small/multiqc/multiqc_plots", + "small/multiqc/multiqc_plots/pdf", + "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", + "small/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "small/multiqc/multiqc_plots/png", + "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png", + "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", + "small/multiqc/multiqc_plots/png/general_stats_table.png", + "small/multiqc/multiqc_plots/svg", + "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg", + "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", + "small/multiqc/multiqc_plots/svg/general_stats_table.svg", "small/multiqc/multiqc_report.html", "small/summary", "small/summary/comparisons", - "small/summary/comparisons/small", - "small/summary/comparisons/small/rtgtools.FN.csv", - "small/summary/comparisons/small/rtgtools.FP.csv", - "small/summary/comparisons/small/rtgtools.TP_base.csv", - "small/summary/comparisons/small/rtgtools.TP_comp.csv", + "small/summary/comparisons/rtgtools.FN.csv", + "small/summary/comparisons/rtgtools.FP.csv", + "small/summary/comparisons/rtgtools.TP_base.csv", + "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", "small/summary/datavzrd/happy", "small/summary/datavzrd/happy/index.html", @@ -421,17 +462,17 @@ "small/summary/datavzrd/versions.yml", "small/summary/plots", "small/summary/plots/happy", - "small/summary/plots/happy/INDEL_ALL_metric_by_tool_happy.png", - "small/summary/plots/happy/INDEL_ALL_variants_by_tool_happy.png", - "small/summary/plots/happy/INDEL_PASS_metric_by_tool_happy.png", - "small/summary/plots/happy/INDEL_PASS_variants_by_tool_happy.png", - "small/summary/plots/happy/SNP_ALL_metric_by_tool_happy.png", - "small/summary/plots/happy/SNP_ALL_variants_by_tool_happy.png", - "small/summary/plots/happy/SNP_PASS_metric_by_tool_happy.png", - "small/summary/plots/happy/SNP_PASS_variants_by_tool_happy.png", + "small/summary/plots/happy/INDEL_ALL_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_ALL_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_PASS_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_PASS_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_ALL_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_ALL_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_PASS_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_PASS_variants_by_tool_happy_mqc.png", "small/summary/plots/rtgtools", - "small/summary/plots/rtgtools/metric_by_tool_rtgtools.png", - "small/summary/plots/rtgtools/variants_by_tool_rtgtools.png", + "small/summary/plots/rtgtools/metric_by_tool_rtgtools_mqc.png", + "small/summary/plots/rtgtools/variants_by_tool_rtgtools_mqc.png", "small/summary/tables", "small/summary/tables/happy", "small/summary/tables/happy/happy.summary.csv", @@ -524,6 +565,10 @@ "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.bcftools_stats.txt:md5,b215fc0030c53bc8887e28b23b97efb6", + "rtgtools.FN.csv:md5,80f23e0271742bc7400804311dd3b437", + "rtgtools.FP.csv:md5,c5229b5b7fd78d90e9a54e64885728e5", + "rtgtools.TP_base.csv:md5,aae4d65a540533faa4f275d0f7e9753a", + "rtgtools.TP_comp.csv:md5,b2d6fa254026b506ef64ccf1f12ab21a", "test1.HG002.strelka.extended.csv:md5,098dc352d1beeab27e72f97e64fc182e", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,bd1f1a1e138f75511de7aae157fa2fa4", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8912022beb3c5b234d29300b764b56bc", @@ -548,8 +593,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-13T08:41:21.414009313" + "timestamp": "2024-11-22T10:11:26.258107754" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index e845a97..6e03e8d 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'": { "content": [ - 138, + 144, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -18,6 +18,9 @@ "BCFTOOLS_STATS": { "bcftools": 1.18 }, + "BCFTOOLS_VIEW_CONTIGS": { + "bcftools": 1.18 + }, "BGZIP_TABIX": { "tabix": 1.12 }, @@ -45,6 +48,9 @@ "TABIX_BGZIP": { "tabix": "1.19.1" }, + "TABIX_BGZIPTABIX": { + "tabix": "1.19.1" + }, "TABIX_BGZIP_QUERY": { "tabix": "1.19.1" }, @@ -81,24 +87,52 @@ "structural/HG002/stats/bcftools", "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", "structural/HG002/stats/survivor", - "structural/HG002/stats/survivor/HG002.stats", + "structural/HG002/stats/survivor/HG002_mqc.stats", "structural/multiqc", "structural/multiqc/multiqc_data", + "structural/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", "structural/multiqc/multiqc_data/multiqc.log", + "structural/multiqc/multiqc_data/multiqc_bcftools_stats.txt", "structural/multiqc/multiqc_data/multiqc_citations.txt", "structural/multiqc/multiqc_data/multiqc_data.json", + "structural/multiqc/multiqc_data/multiqc_general_stats.txt", "structural/multiqc/multiqc_data/multiqc_software_versions.txt", "structural/multiqc/multiqc_data/multiqc_sources.txt", + "structural/multiqc/multiqc_plots", + "structural/multiqc/multiqc_plots/pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", + "structural/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "structural/multiqc/multiqc_plots/png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", + "structural/multiqc/multiqc_plots/png/general_stats_table.png", + "structural/multiqc/multiqc_plots/svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", + "structural/multiqc/multiqc_plots/svg/general_stats_table.svg", "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", - "structural/summary/comparisons/structural", - "structural/summary/comparisons/structural/svbenchmark.FN.csv", - "structural/summary/comparisons/structural/svbenchmark.FP.csv", - "structural/summary/comparisons/structural/truvari.FN.csv", - "structural/summary/comparisons/structural/truvari.FP.csv", - "structural/summary/comparisons/structural/truvari.TP_base.csv", - "structural/summary/comparisons/structural/truvari.TP_comp.csv", + "structural/summary/comparisons/svbenchmark.FN.csv", + "structural/summary/comparisons/svbenchmark.FP.csv", + "structural/summary/comparisons/truvari.FN.csv", + "structural/summary/comparisons/truvari.FP.csv", + "structural/summary/comparisons/truvari.TP_base.csv", + "structural/summary/comparisons/truvari.TP_comp.csv", "structural/summary/datavzrd", "structural/summary/datavzrd/svbenchmark", "structural/summary/datavzrd/svbenchmark/index.html", @@ -179,16 +213,16 @@ "structural/summary/datavzrd/wittyer/test/plots/plot_8.js", "structural/summary/plots", "structural/summary/plots/svbenchmark", - "structural/summary/plots/svbenchmark/metric_by_tool_svbenchmark.png", - "structural/summary/plots/svbenchmark/variants_by_tool_svbenchmark.png", + "structural/summary/plots/svbenchmark/metric_by_tool_svbenchmark_mqc.png", + "structural/summary/plots/svbenchmark/variants_by_tool_svbenchmark_mqc.png", "structural/summary/plots/truvari", - "structural/summary/plots/truvari/metric_by_tool_truvari.png", - "structural/summary/plots/truvari/variants_by_tool_truvari.png", + "structural/summary/plots/truvari/metric_by_tool_truvari_mqc.png", + "structural/summary/plots/truvari/variants_by_tool_truvari_mqc.png", "structural/summary/plots/wittyer", - "structural/summary/plots/wittyer/Base_metric_by_tool_wittyer.png", - "structural/summary/plots/wittyer/Base_variants_by_tool_wittyer.png", - "structural/summary/plots/wittyer/Event_metric_by_tool_wittyer.png", - "structural/summary/plots/wittyer/Event_variants_by_tool_wittyer.png", + "structural/summary/plots/wittyer/Base_metric_by_tool_wittyer_mqc.png", + "structural/summary/plots/wittyer/Base_variants_by_tool_wittyer_mqc.png", + "structural/summary/plots/wittyer/Event_metric_by_tool_wittyer_mqc.png", + "structural/summary/plots/wittyer/Event_variants_by_tool_wittyer_mqc.png", "structural/summary/tables", "structural/summary/tables/svbenchmark", "structural/summary/tables/svbenchmark/svbenchmark.summary.csv", @@ -214,21 +248,21 @@ "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-base.vcf.gz.tbi", "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-comp.vcf.gz", "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-comp.vcf.gz.tbi", - "structural/test1/benchmarks/wittyer_bench", - "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.json", - "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.vcf.gz", - "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.vcf.gz.tbi", + "structural/test1/benchmarks/wittyer", + "structural/test1/benchmarks/wittyer/test1.HG002.manta.json", + "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz", + "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz.tbi", "structural/test1/preprocess", "structural/test1/preprocess/manta.HG002.chr21.norm.sort.vcf.gz", "structural/test1/preprocess/manta.HG002.chr21.norm.vcf.gz", "structural/test1/preprocess/test1.dedup.sort.vcf.gz", - "structural/test1/preprocess/test1.manta.svync.rh.norm.filter.vcf", "structural/test1/preprocess/test1.manta.svync.vcf.gz", + "structural/test1/preprocess/test1.norm.filter.vcf", "structural/test1/stats", "structural/test1/stats/bcftools", "structural/test1/stats/bcftools/test1.manta.bcftools_stats.txt", "structural/test1/stats/survivor", - "structural/test1/stats/survivor/test1.manta.stats", + "structural/test1/stats/survivor/test1.manta_mqc.stats", "structural/test2", "structural/test2/benchmarks", "structural/test2/benchmarks/svanalyzer", @@ -247,20 +281,20 @@ "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-base.vcf.gz.tbi", "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz", "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz.tbi", - "structural/test2/benchmarks/wittyer_bench", - "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.json", - "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.vcf.gz", - "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.vcf.gz.tbi", + "structural/test2/benchmarks/wittyer", + "structural/test2/benchmarks/wittyer/test2.HG002.merged.json", + "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz", + "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz.tbi", "structural/test2/preprocess", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.sort.vcf.gz", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.vcf.gz", "structural/test2/preprocess/test2.dedup.sort.vcf.gz", - "structural/test2/preprocess/test2.rh.norm.filter.vcf", + "structural/test2/preprocess/test2.norm.filter.vcf", "structural/test2/stats", "structural/test2/stats/bcftools", "structural/test2/stats/bcftools/test2.merged.bcftools_stats.txt", "structural/test2/stats/survivor", - "structural/test2/stats/survivor/test2.merged.stats", + "structural/test2/stats/survivor/test2.merged_mqc.stats", "structural/test3", "structural/test3/benchmarks", "structural/test3/benchmarks/svanalyzer", @@ -279,48 +313,54 @@ "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-base.vcf.gz.tbi", "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-comp.vcf.gz", "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-comp.vcf.gz.tbi", - "structural/test3/benchmarks/wittyer_bench", - "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.json", - "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.vcf.gz", - "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.vcf.gz.tbi", + "structural/test3/benchmarks/wittyer", + "structural/test3/benchmarks/wittyer/test3.HG002.dragen.json", + "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz", + "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz.tbi", "structural/test3/preprocess", "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.sort.vcf.gz", "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", "structural/test3/preprocess/test3.dedup.sort.vcf.gz", - "structural/test3/preprocess/test3.dragen.svync.rh.norm.filter.vcf", "structural/test3/preprocess/test3.dragen.svync.vcf.gz", + "structural/test3/preprocess/test3.norm.filter.vcf", "structural/test3/stats", "structural/test3/stats/bcftools", "structural/test3/stats/bcftools/test3.dragen.bcftools_stats.txt", "structural/test3/stats/survivor", - "structural/test3/stats/survivor/test3.dragen.stats" + "structural/test3/stats/survivor/test3.dragen_mqc.stats" ], [ "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", - "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", + "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", + "svbenchmark.FN.csv:md5,fda320e4b95452acedfdc8cc3a472ad3", + "svbenchmark.FP.csv:md5,35068a0c16903cba32257a8d9a6d51a2", + "truvari.FN.csv:md5,7d2992f331488dd307831a25b8157b0a", + "truvari.FP.csv:md5,cdc258e9153f7a75fc8340ce7211b577", + "truvari.TP_base.csv:md5,cd6d6a9c870739378c7e0ecc6868ebf6", + "truvari.TP_comp.csv:md5,4a47608038a1301cb518d872619245ff", "test1.HG002.manta.distances:md5,33dcbe29f7129dc30c184b564803ec63", "test1.HG002.manta.report:md5,91b0fbfd6fcdf45b85290f92c5a470fb", "test1.manta.bcftools_stats.txt:md5,0e4cf1707b245c026fe6a0af6e8f8c0a", - "test1.manta.stats:md5,6f25a9372095052218110390e8e75c54", + "test1.manta_mqc.stats:md5,6f25a9372095052218110390e8e75c54", "test2.HG002.merged.distances:md5,346f18a5cbeece98716951c8fc2aaea4", "test2.HG002.merged.report:md5,6466d73155f88084efc69cad3628eb8c", "test2.merged.bcftools_stats.txt:md5,576f9bc7e06bc2bbfccceb9da161a467", - "test2.merged.stats:md5,36d80468e13b583d0761b434d095312d", + "test2.merged_mqc.stats:md5,36d80468e13b583d0761b434d095312d", "test3.HG002.dragen.distances:md5,346f18a5cbeece98716951c8fc2aaea4", "test3.HG002.dragen.report:md5,b295d4867b7a96f0ca0bb5e8bb45eb68", "test3.dragen.bcftools_stats.txt:md5,0683a5d3c47a5a6a43ad2b6a0387672e", - "test3.dragen.stats:md5,5c59cceb2e7d909d91074bb8e5804abc" + "test3.dragen_mqc.stats:md5,5c59cceb2e7d909d91074bb8e5804abc" ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T15:58:48.350235224" + "timestamp": "2024-11-22T10:40:00.667995493" }, "-stub": { "content": [ - 138, + 144, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -337,6 +377,9 @@ "BCFTOOLS_STATS": { "bcftools": 1.18 }, + "BCFTOOLS_VIEW_CONTIGS": { + "bcftools": 1.18 + }, "BGZIP_TABIX": { "tabix": 1.12 }, @@ -364,6 +407,9 @@ "TABIX_BGZIP": { "tabix": "1.19.1" }, + "TABIX_BGZIPTABIX": { + "tabix": "1.19.1" + }, "TABIX_BGZIP_QUERY": { "tabix": "1.19.1" }, @@ -400,20 +446,19 @@ "structural/HG002/stats/bcftools", "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", "structural/HG002/stats/survivor", - "structural/HG002/stats/survivor/HG002.stats", + "structural/HG002/stats/survivor/HG002_mqc.stats", "structural/multiqc", "structural/multiqc/multiqc_data", "structural/multiqc/multiqc_plots", "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", - "structural/summary/comparisons/structural", - "structural/summary/comparisons/structural/svbenchmark.FN.csv", - "structural/summary/comparisons/structural/svbenchmark.FP.csv", - "structural/summary/comparisons/structural/truvari.FN.csv", - "structural/summary/comparisons/structural/truvari.FP.csv", - "structural/summary/comparisons/structural/truvari.TP_base.csv", - "structural/summary/comparisons/structural/truvari.TP_comp.csv", + "structural/summary/comparisons/svbenchmark.FN.csv", + "structural/summary/comparisons/svbenchmark.FP.csv", + "structural/summary/comparisons/truvari.FN.csv", + "structural/summary/comparisons/truvari.FP.csv", + "structural/summary/comparisons/truvari.TP_base.csv", + "structural/summary/comparisons/truvari.TP_comp.csv", "structural/summary/datavzrd", "structural/summary/datavzrd/svbenchmark", "structural/summary/datavzrd/svbenchmark/index.html", @@ -472,14 +517,14 @@ "structural/summary/datavzrd/wittyer/static/datavzrd.css", "structural/summary/plots", "structural/summary/plots/svbenchmark", - "structural/summary/plots/svbenchmark/metric_by_tool_svbenchmark.png", - "structural/summary/plots/svbenchmark/variants_by_tool_svbenchmark.png", + "structural/summary/plots/svbenchmark/metric_by_tool_svbenchmark_mqc.png", + "structural/summary/plots/svbenchmark/variants_by_tool_svbenchmark_mqc.png", "structural/summary/plots/truvari", - "structural/summary/plots/truvari/metric_by_tool_truvari.png", - "structural/summary/plots/truvari/variants_by_tool_truvari.png", + "structural/summary/plots/truvari/metric_by_tool_truvari_mqc.png", + "structural/summary/plots/truvari/variants_by_tool_truvari_mqc.png", "structural/summary/plots/wittyer", - "structural/summary/plots/wittyer/metric_by_tool_wittyer.png", - "structural/summary/plots/wittyer/variants_by_tool_wittyer.png", + "structural/summary/plots/wittyer/metric_by_tool_wittyer_mqc.png", + "structural/summary/plots/wittyer/variants_by_tool_wittyer_mqc.png", "structural/summary/tables", "structural/summary/tables/svbenchmark", "structural/summary/tables/svbenchmark/svbenchmark.regions.csv", @@ -508,21 +553,21 @@ "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-base.vcf.gz.tbi", "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-comp.vcf.gz", "structural/test1/benchmarks/truvari/test1.HG002.manta.tp-comp.vcf.gz.tbi", - "structural/test1/benchmarks/wittyer_bench", - "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.json", - "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.vcf.gz", - "structural/test1/benchmarks/wittyer_bench/test1.HG002.manta.vcf.gz.tbi", + "structural/test1/benchmarks/wittyer", + "structural/test1/benchmarks/wittyer/test1.HG002.manta.json", + "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz", + "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz.tbi", "structural/test1/preprocess", "structural/test1/preprocess/manta.HG002.chr21.norm.sort.vcf.gz", "structural/test1/preprocess/manta.HG002.chr21.norm.vcf.gz", "structural/test1/preprocess/test1.dedup.sort.vcf.gz", - "structural/test1/preprocess/test1.manta.svync.rh.norm.filter.vcf", "structural/test1/preprocess/test1.manta.svync.vcf.gz", + "structural/test1/preprocess/test1.norm.filter.vcf", "structural/test1/stats", "structural/test1/stats/bcftools", "structural/test1/stats/bcftools/test1.manta.bcftools_stats.txt", "structural/test1/stats/survivor", - "structural/test1/stats/survivor/test1.manta.stats", + "structural/test1/stats/survivor/test1.manta_mqc.stats", "structural/test2", "structural/test2/benchmarks", "structural/test2/benchmarks/svanalyzer", @@ -541,20 +586,20 @@ "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-base.vcf.gz.tbi", "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz", "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz.tbi", - "structural/test2/benchmarks/wittyer_bench", - "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.json", - "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.vcf.gz", - "structural/test2/benchmarks/wittyer_bench/test2.HG002.merged.vcf.gz.tbi", + "structural/test2/benchmarks/wittyer", + "structural/test2/benchmarks/wittyer/test2.HG002.merged.json", + "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz", + "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz.tbi", "structural/test2/preprocess", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.sort.vcf.gz", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.vcf.gz", "structural/test2/preprocess/test2.dedup.sort.vcf.gz", - "structural/test2/preprocess/test2.rh.norm.filter.vcf", + "structural/test2/preprocess/test2.norm.filter.vcf", "structural/test2/stats", "structural/test2/stats/bcftools", "structural/test2/stats/bcftools/test2.merged.bcftools_stats.txt", "structural/test2/stats/survivor", - "structural/test2/stats/survivor/test2.merged.stats", + "structural/test2/stats/survivor/test2.merged_mqc.stats", "structural/test3", "structural/test3/benchmarks", "structural/test3/benchmarks/svanalyzer", @@ -573,26 +618,31 @@ "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-base.vcf.gz.tbi", "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-comp.vcf.gz", "structural/test3/benchmarks/truvari/test3.HG002.dragen.tp-comp.vcf.gz.tbi", - "structural/test3/benchmarks/wittyer_bench", - "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.json", - "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.vcf.gz", - "structural/test3/benchmarks/wittyer_bench/test3.HG002.dragen.vcf.gz.tbi", + "structural/test3/benchmarks/wittyer", + "structural/test3/benchmarks/wittyer/test3.HG002.dragen.json", + "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz", + "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz.tbi", "structural/test3/preprocess", "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.sort.vcf.gz", "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", "structural/test3/preprocess/test3.dedup.sort.vcf.gz", - "structural/test3/preprocess/test3.dragen.svync.rh.norm.filter.vcf", "structural/test3/preprocess/test3.dragen.svync.vcf.gz", + "structural/test3/preprocess/test3.norm.filter.vcf", "structural/test3/stats", "structural/test3/stats/bcftools", "structural/test3/stats/bcftools/test3.dragen.bcftools_stats.txt", "structural/test3/stats/survivor", - "structural/test3/stats/survivor/test3.dragen.stats" + "structural/test3/stats/survivor/test3.dragen_mqc.stats" ], [ "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "HG002.stats:md5,d41d8cd98f00b204e9800998ecf8427e", - "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", + "HG002_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "svbenchmark.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "svbenchmark.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -614,21 +664,21 @@ "test1.HG002.manta.distances:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.manta.report:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.manta.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "test1.manta_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.HG002.merged.distances:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.HG002.merged.report:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.merged.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.merged.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.merged_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.HG002.dragen.distances:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.HG002.dragen.report:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.dragen.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.dragen.stats:md5,d41d8cd98f00b204e9800998ecf8427e" + "test3.dragen_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:01:22.753474251" + "timestamp": "2024-11-22T10:42:21.212792945" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index bde2593..f3dacf1 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -105,19 +105,58 @@ "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", "small/multiqc", "small/multiqc/multiqc_data", + "small/multiqc/multiqc_data/bcftools-stats-subtypes.txt", + "small/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "small/multiqc/multiqc_data/bcftools_stats_variant_depths.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", "small/multiqc/multiqc_data/multiqc.log", + "small/multiqc/multiqc_data/multiqc_bcftools_stats.txt", "small/multiqc/multiqc_data/multiqc_citations.txt", "small/multiqc/multiqc_data/multiqc_data.json", + "small/multiqc/multiqc_data/multiqc_general_stats.txt", "small/multiqc/multiqc_data/multiqc_software_versions.txt", "small/multiqc/multiqc_data/multiqc_sources.txt", + "small/multiqc/multiqc_plots", + "small/multiqc/multiqc_plots/pdf", + "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", + "small/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "small/multiqc/multiqc_plots/png", + "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png", + "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", + "small/multiqc/multiqc_plots/png/general_stats_table.png", + "small/multiqc/multiqc_plots/svg", + "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg", + "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", + "small/multiqc/multiqc_plots/svg/general_stats_table.svg", "small/multiqc/multiqc_report.html", "small/summary", "small/summary/comparisons", - "small/summary/comparisons/small", - "small/summary/comparisons/small/rtgtools.FN.csv", - "small/summary/comparisons/small/rtgtools.FP.csv", - "small/summary/comparisons/small/rtgtools.TP_base.csv", - "small/summary/comparisons/small/rtgtools.TP_comp.csv", + "small/summary/comparisons/rtgtools.FN.csv", + "small/summary/comparisons/rtgtools.FP.csv", + "small/summary/comparisons/rtgtools.TP_base.csv", + "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", "small/summary/datavzrd/happy", "small/summary/datavzrd/happy/index.html", @@ -184,17 +223,17 @@ "small/summary/datavzrd/versions.yml", "small/summary/plots", "small/summary/plots/happy", - "small/summary/plots/happy/INDEL_ALL_metric_by_tool_happy.png", - "small/summary/plots/happy/INDEL_ALL_variants_by_tool_happy.png", - "small/summary/plots/happy/INDEL_PASS_metric_by_tool_happy.png", - "small/summary/plots/happy/INDEL_PASS_variants_by_tool_happy.png", - "small/summary/plots/happy/SNP_ALL_metric_by_tool_happy.png", - "small/summary/plots/happy/SNP_ALL_variants_by_tool_happy.png", - "small/summary/plots/happy/SNP_PASS_metric_by_tool_happy.png", - "small/summary/plots/happy/SNP_PASS_variants_by_tool_happy.png", + "small/summary/plots/happy/INDEL_ALL_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_ALL_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_PASS_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_PASS_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_ALL_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_ALL_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_PASS_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_PASS_variants_by_tool_happy_mqc.png", "small/summary/plots/rtgtools", - "small/summary/plots/rtgtools/metric_by_tool_rtgtools.png", - "small/summary/plots/rtgtools/variants_by_tool_rtgtools.png", + "small/summary/plots/rtgtools/metric_by_tool_rtgtools_mqc.png", + "small/summary/plots/rtgtools/variants_by_tool_rtgtools_mqc.png", "small/summary/tables", "small/summary/tables/happy", "small/summary/tables/happy/happy.summary.csv", @@ -286,6 +325,10 @@ "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", + "rtgtools.FN.csv:md5,fb46816d5d479287984c4005fdd1631a", + "rtgtools.FP.csv:md5,24761919bc77724a3e1582e3bbee01cb", + "rtgtools.TP_base.csv:md5,ed42cd3e248812c1a3e1cf4985b92539", + "rtgtools.TP_comp.csv:md5,49259e81f11d9014c4ccea064a1a05b1", "test1.HG002.strelka.extended.csv:md5,fadf06276179c1cc388007e5651fb9af", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a08665be8ea20855a14d6418d07521dc", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8d4a20a5914379230952aeb252845e2d", @@ -310,9 +353,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-13T09:00:38.410760165" + "timestamp": "2024-11-22T10:25:06.723892719" }, "-stub": { "content": [ diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 7376835..e4936e4 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -51,11 +51,51 @@ "indel/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", "indel/multiqc", "indel/multiqc/multiqc_data", + "indel/multiqc/multiqc_data/bcftools-stats-subtypes.txt", + "indel/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "indel/multiqc/multiqc_data/bcftools_stats_variant_depths.txt", + "indel/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "indel/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "indel/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "indel/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", "indel/multiqc/multiqc_data/multiqc.log", + "indel/multiqc/multiqc_data/multiqc_bcftools_stats.txt", "indel/multiqc/multiqc_data/multiqc_citations.txt", "indel/multiqc/multiqc_data/multiqc_data.json", + "indel/multiqc/multiqc_data/multiqc_general_stats.txt", "indel/multiqc/multiqc_data/multiqc_software_versions.txt", "indel/multiqc/multiqc_data/multiqc_sources.txt", + "indel/multiqc/multiqc_plots", + "indel/multiqc/multiqc_plots/pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "indel/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", + "indel/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "indel/multiqc/multiqc_plots/png", + "indel/multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png", + "indel/multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png", + "indel/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "indel/multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png", + "indel/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "indel/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "indel/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "indel/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", + "indel/multiqc/multiqc_plots/png/general_stats_table.png", + "indel/multiqc/multiqc_plots/svg", + "indel/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg", + "indel/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg", + "indel/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "indel/multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg", + "indel/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "indel/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "indel/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "indel/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", + "indel/multiqc/multiqc_plots/svg/general_stats_table.svg", "indel/multiqc/multiqc_report.html", "indel/summary", "indel/summary/datavzrd", @@ -98,8 +138,8 @@ "indel/summary/datavzrd/versions.yml", "indel/summary/plots", "indel/summary/plots/sompy", - "indel/summary/plots/sompy/metric_by_tool_sompy.png", - "indel/summary/plots/sompy/variants_by_tool_sompy.png", + "indel/summary/plots/sompy/metric_by_tool_sompy_mqc.png", + "indel/summary/plots/sompy/variants_by_tool_sompy_mqc.png", "indel/summary/tables", "indel/summary/tables/sompy", "indel/summary/tables/sompy/sompy.regions.csv", @@ -139,8 +179,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:14:37.608031284" + "timestamp": "2024-11-22T10:27:18.206645077" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 278af4a..c0e5601 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -1,115 +1,26 @@ { "-stub": { "content": [ - 38, - { - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.18 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_FILTERMISSING": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_SUBSAMPLE": { - "bcftools": 1.18 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "HAPPY_SOMPY": { - "hap.py": "0.3.14" - }, - "MERGE_REPORTS": { - "python": "3.8.6" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + 30, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "snv", "snv/SEQC2", "snv/SEQC2/stats", "snv/SEQC2/stats/bcftools", "snv/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", - "snv/multiqc", - "snv/multiqc/multiqc_data", - "snv/multiqc/multiqc_plots", - "snv/multiqc/multiqc_report.html", - "snv/summary", - "snv/summary/datavzrd", - "snv/summary/datavzrd/sompy", - "snv/summary/datavzrd/sompy/index.html", - "snv/summary/datavzrd/sompy/network", - "snv/summary/datavzrd/sompy/network/config.js", - "snv/summary/datavzrd/sompy/network/data", - "snv/summary/datavzrd/sompy/network/data/data_1.js", - "snv/summary/datavzrd/sompy/network/functions.js", - "snv/summary/datavzrd/sompy/network/heatmap.js", - "snv/summary/datavzrd/sompy/network/index_1.html", - "snv/summary/datavzrd/sompy/network/plots", - "snv/summary/datavzrd/sompy/network/plots/plot_0.js", - "snv/summary/datavzrd/sompy/static", - "snv/summary/datavzrd/sompy/static/bootstrap-select.min.css", - "snv/summary/datavzrd/sompy/static/bootstrap-table-fixed-columns.min.css", - "snv/summary/datavzrd/sompy/static/bootstrap-table.min.css", - "snv/summary/datavzrd/sompy/static/bootstrap.min.css", - "snv/summary/datavzrd/sompy/static/bundle.js", - "snv/summary/datavzrd/sompy/static/datavzrd.css", - "snv/summary/datavzrd/versions.yml", - "snv/summary/plots", - "snv/summary/plots/sompy", - "snv/summary/plots/sompy/metric_by_tool_sompy.png", - "snv/summary/plots/sompy/variants_by_tool_sompy.png", - "snv/summary/tables", - "snv/summary/tables/sompy", - "snv/summary/tables/sompy/sompy.regions.csv", - "snv/summary/tables/sompy/sompy.summary.csv", "snv/test1", - "snv/test1/benchmarks", - "snv/test1/benchmarks/sompy", - "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.features.csv", - "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.metrics.json", - "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.stats.csv", "snv/test1/stats", "snv/test1/stats/bcftools", "snv/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", "snv/test2", - "snv/test2/benchmarks", - "snv/test2/benchmarks/sompy", - "snv/test2/benchmarks/sompy/test2.SEQC2.manta.features.csv", - "snv/test2/benchmarks/sompy/test2.SEQC2.manta.metrics.json", - "snv/test2/benchmarks/sompy/test2.SEQC2.manta.stats.csv", "snv/test2/preprocess", "snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", "snv/test2/stats", "snv/test2/stats/bcftools", "snv/test2/stats/bcftools/test2.manta.bcftools_stats.txt", "snv/test3", - "snv/test3/benchmarks", - "snv/test3/benchmarks/sompy", - "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.features.csv", - "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.metrics.json", - "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.stats.csv", "snv/test3/preprocess", "snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", "snv/test3/stats", @@ -118,28 +29,15 @@ ], [ "SEQC2.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.SEQC2.freebayes.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.SEQC2.freebayes.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.freebayes.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.SEQC2.manta.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.SEQC2.manta.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.SEQC2.strelka.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.SEQC2.strelka.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.strelka.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:24:28.108507744" + "timestamp": "2024-11-22T10:30:59.285037668" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index 331d865..a6dbdde 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -67,22 +67,50 @@ "structural/SEQC2/stats/bcftools", "structural/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", "structural/SEQC2/stats/survivor", - "structural/SEQC2/stats/survivor/SEQC2.stats", + "structural/SEQC2/stats/survivor/SEQC2_mqc.stats", "structural/multiqc", "structural/multiqc/multiqc_data", + "structural/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", "structural/multiqc/multiqc_data/multiqc.log", + "structural/multiqc/multiqc_data/multiqc_bcftools_stats.txt", "structural/multiqc/multiqc_data/multiqc_citations.txt", "structural/multiqc/multiqc_data/multiqc_data.json", + "structural/multiqc/multiqc_data/multiqc_general_stats.txt", "structural/multiqc/multiqc_data/multiqc_software_versions.txt", "structural/multiqc/multiqc_data/multiqc_sources.txt", + "structural/multiqc/multiqc_plots", + "structural/multiqc/multiqc_plots/pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", + "structural/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "structural/multiqc/multiqc_plots/png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", + "structural/multiqc/multiqc_plots/png/general_stats_table.png", + "structural/multiqc/multiqc_plots/svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", + "structural/multiqc/multiqc_plots/svg/general_stats_table.svg", "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", - "structural/summary/comparisons/structural", - "structural/summary/comparisons/structural/truvari.FN.csv", - "structural/summary/comparisons/structural/truvari.FP.csv", - "structural/summary/comparisons/structural/truvari.TP_base.csv", - "structural/summary/comparisons/structural/truvari.TP_comp.csv", + "structural/summary/comparisons/truvari.FN.csv", + "structural/summary/comparisons/truvari.FP.csv", + "structural/summary/comparisons/truvari.TP_base.csv", + "structural/summary/comparisons/truvari.TP_comp.csv", "structural/summary/datavzrd", "structural/summary/datavzrd/truvari", "structural/summary/datavzrd/truvari/index.html", @@ -112,8 +140,8 @@ "structural/summary/datavzrd/versions.yml", "structural/summary/plots", "structural/summary/plots/truvari", - "structural/summary/plots/truvari/metric_by_tool_truvari.png", - "structural/summary/plots/truvari/variants_by_tool_truvari.png", + "structural/summary/plots/truvari/metric_by_tool_truvari_mqc.png", + "structural/summary/plots/truvari/variants_by_tool_truvari_mqc.png", "structural/summary/tables", "structural/summary/tables/truvari", "structural/summary/tables/truvari/truvari.summary.csv", @@ -136,7 +164,7 @@ "structural/test1/stats/bcftools", "structural/test1/stats/bcftools/test1.tiddit.bcftools_stats.txt", "structural/test1/stats/survivor", - "structural/test1/stats/survivor/test1.tiddit.stats", + "structural/test1/stats/survivor/test1.tiddit_mqc.stats", "structural/test2", "structural/test2/benchmarks", "structural/test2/benchmarks/truvari", @@ -156,21 +184,25 @@ "structural/test2/stats/bcftools", "structural/test2/stats/bcftools/test2.manta.bcftools_stats.txt", "structural/test2/stats/survivor", - "structural/test2/stats/survivor/test2.manta.stats" + "structural/test2/stats/survivor/test2.manta_mqc.stats" ], [ "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", - "SEQC2.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", + "SEQC2_mqc.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", + "truvari.FN.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", + "truvari.FP.csv:md5,d47360e5f1260a7d1cfa1797a7814ef8", + "truvari.TP_base.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", + "truvari.TP_comp.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", - "test1.tiddit.stats:md5,0eb749429a6072f6bf63d377e37514d1", + "test1.tiddit_mqc.stats:md5,0eb749429a6072f6bf63d377e37514d1", "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", - "test2.manta.stats:md5,73bc60cd2754202ddca8be6552e85ffa" + "test2.manta_mqc.stats:md5,73bc60cd2754202ddca8be6552e85ffa" ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:27:02.901069711" + "timestamp": "2024-11-22T10:35:32.636046624" } } \ No newline at end of file From cecd72ae5bdf5d1d9503b52e44678bd9f77475e8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 22 Nov 2024 12:43:26 +0000 Subject: [PATCH 206/292] updates on snapshot --- tests/.nftignore | 17 ++++-- tests/germline_sv.nf.test.snap | 34 +---------- tests/somatic_snv.nf.test.snap | 101 ++++++++++++++++++++++++++++++++- 3 files changed, 112 insertions(+), 40 deletions(-) diff --git a/tests/.nftignore b/tests/.nftignore index 148cde2..ee8bdcf 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -9,17 +9,24 @@ references/rtgtools/genome.sdf/progress references/rtgtools/genome.sdf/format.log references/rtgtools/genome.sdf/mainIndex references/rtgtools/genome.sdf/done -**/summary/tables/**/*.csv **/summary/datavzrd/versions.yml **/summary/datavzrd/**/index.html **/summary/datavzrd/**/static/* -**/summary/datavzrd/**/test/*.js +**/summary/datavzrd/**/static/*.{js} +**/summary/datavzrd/**/static/*.{css} +**/summary/datavzrd/**/test/*.{js} **/summary/datavzrd/**/test/index_1.html **/summary/datavzrd/**/test/data/data_1.js -**/summary/datavzrd/**/test/plots/*.js +**/summary/datavzrd/**/test/plots/*.{js} +**/summary/datavzrd/**/network/plots/*.{js} +**/summary/datavzrd/**/network/*.{js} +**/summary/datavzrd/**/network/index_1.html +**/summary/datavzrd/**/network/data/data_1.js **/summary/plots/**/*.png -**/summary/comparisons/**/*.csv +**/summary/comparisons/*.csv +**/summary/tables/**/*.csv **/**/benchmarks/**/*.{json,json.gz,tsv.gz,vcf.gz,gz.tbi} -**/**/benchmarks/svanalyzer/*.{log} +**/**/benchmarks/svanalyzer/*.log +**/**/benchmarks/sompy/*.csv **/**/preprocess/*.{vcf.gz,gz.tbi,vcf} **/**/liftover/*.{vcf.gz,gz.tbi,vcf} diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index 6e03e8d..b04185a 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -332,12 +332,6 @@ [ "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "svbenchmark.FN.csv:md5,fda320e4b95452acedfdc8cc3a472ad3", - "svbenchmark.FP.csv:md5,35068a0c16903cba32257a8d9a6d51a2", - "truvari.FN.csv:md5,7d2992f331488dd307831a25b8157b0a", - "truvari.FP.csv:md5,cdc258e9153f7a75fc8340ce7211b577", - "truvari.TP_base.csv:md5,cd6d6a9c870739378c7e0ecc6868ebf6", - "truvari.TP_comp.csv:md5,4a47608038a1301cb518d872619245ff", "test1.HG002.manta.distances:md5,33dcbe29f7129dc30c184b564803ec63", "test1.HG002.manta.report:md5,91b0fbfd6fcdf45b85290f92c5a470fb", "test1.manta.bcftools_stats.txt:md5,0e4cf1707b245c026fe6a0af6e8f8c0a", @@ -356,7 +350,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:40:00.667995493" + "timestamp": "2024-11-22T12:36:40.054294704" }, "-stub": { "content": [ @@ -637,30 +631,6 @@ [ "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "HG002_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", - "svbenchmark.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "svbenchmark.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.manta.distances:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.manta.report:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -679,6 +649,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:42:21.212792945" + "timestamp": "2024-11-22T12:38:58.811950484" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index c0e5601..e71e9d7 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -1,26 +1,115 @@ { "-stub": { "content": [ - 30, - null, + 38, + { + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_SORT": { + "bcftools": 1.18 + }, + "BCFTOOLS_STATS": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_CONTIGS": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_FILTERMISSING": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_SUBSAMPLE": { + "bcftools": 1.18 + }, + "DATAVZRD": { + "datavzrd": "2.36.12" + }, + "HAPPY_SOMPY": { + "hap.py": "0.3.14" + }, + "MERGE_REPORTS": { + "python": "3.8.6" + }, + "PLOTS": { + "r-base": "4.3.1" + }, + "TABIX_BGZIP": { + "tabix": "1.19.1" + }, + "TABIX_BGZIPTABIX": { + "tabix": "1.19.1" + }, + "Workflow": { + "nf-core/variantbenchmarking": "v1.0dev" + } + }, [ "pipeline_info", + "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "snv", "snv/SEQC2", "snv/SEQC2/stats", "snv/SEQC2/stats/bcftools", "snv/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", + "snv/multiqc", + "snv/multiqc/multiqc_data", + "snv/multiqc/multiqc_plots", + "snv/multiqc/multiqc_report.html", + "snv/summary", + "snv/summary/datavzrd", + "snv/summary/datavzrd/sompy", + "snv/summary/datavzrd/sompy/index.html", + "snv/summary/datavzrd/sompy/network", + "snv/summary/datavzrd/sompy/network/config.js", + "snv/summary/datavzrd/sompy/network/data", + "snv/summary/datavzrd/sompy/network/data/data_1.js", + "snv/summary/datavzrd/sompy/network/functions.js", + "snv/summary/datavzrd/sompy/network/heatmap.js", + "snv/summary/datavzrd/sompy/network/index_1.html", + "snv/summary/datavzrd/sompy/network/plots", + "snv/summary/datavzrd/sompy/network/plots/plot_0.js", + "snv/summary/datavzrd/sompy/static", + "snv/summary/datavzrd/sompy/static/bootstrap-select.min.css", + "snv/summary/datavzrd/sompy/static/bootstrap-table-fixed-columns.min.css", + "snv/summary/datavzrd/sompy/static/bootstrap-table.min.css", + "snv/summary/datavzrd/sompy/static/bootstrap.min.css", + "snv/summary/datavzrd/sompy/static/bundle.js", + "snv/summary/datavzrd/sompy/static/datavzrd.css", + "snv/summary/datavzrd/versions.yml", + "snv/summary/plots", + "snv/summary/plots/sompy", + "snv/summary/plots/sompy/metric_by_tool_sompy_mqc.png", + "snv/summary/plots/sompy/variants_by_tool_sompy_mqc.png", + "snv/summary/tables", + "snv/summary/tables/sompy", + "snv/summary/tables/sompy/sompy.regions.csv", + "snv/summary/tables/sompy/sompy.summary.csv", "snv/test1", + "snv/test1/benchmarks", + "snv/test1/benchmarks/sompy", + "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.features.csv", + "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.metrics.json", + "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.stats.csv", "snv/test1/stats", "snv/test1/stats/bcftools", "snv/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", "snv/test2", + "snv/test2/benchmarks", + "snv/test2/benchmarks/sompy", + "snv/test2/benchmarks/sompy/test2.SEQC2.manta.features.csv", + "snv/test2/benchmarks/sompy/test2.SEQC2.manta.metrics.json", + "snv/test2/benchmarks/sompy/test2.SEQC2.manta.stats.csv", "snv/test2/preprocess", "snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", "snv/test2/stats", "snv/test2/stats/bcftools", "snv/test2/stats/bcftools/test2.manta.bcftools_stats.txt", "snv/test3", + "snv/test3/benchmarks", + "snv/test3/benchmarks/sompy", + "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.features.csv", + "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.metrics.json", + "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.stats.csv", "snv/test3/preprocess", "snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", "snv/test3/stats", @@ -29,8 +118,14 @@ ], [ "SEQC2.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "test1.SEQC2.freebayes.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "test1.SEQC2.freebayes.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.freebayes.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.SEQC2.manta.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.SEQC2.manta.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "test3.SEQC2.strelka.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "test3.SEQC2.strelka.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.strelka.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], @@ -38,6 +133,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:30:59.285037668" + "timestamp": "2024-11-22T11:08:48.302568675" } } \ No newline at end of file From 6d9189d32056f00aaa5a4d565cbf5b8c502d92b5 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 22 Nov 2024 13:45:29 +0000 Subject: [PATCH 207/292] update on snapshots --- tests/germline_small.nf.test.snap | 24 ++---------------------- tests/liftover_hg38.nf.test.snap | 6 +----- tests/somatic_indel.nf.test.snap | 6 +----- tests/somatic_snv.nf.test.snap | 8 +------- tests/somatic_sv.nf.test.snap | 6 +----- 5 files changed, 6 insertions(+), 44 deletions(-) diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 7fe0713..019291b 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -205,22 +205,6 @@ [ "genome.sdf:md5,d41d8cd98f00b204e9800998ecf8427e", "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.strelka.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", @@ -247,7 +231,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:13:33.920466332" + "timestamp": "2024-11-22T13:15:43.288411916" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -565,10 +549,6 @@ "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.bcftools_stats.txt:md5,b215fc0030c53bc8887e28b23b97efb6", - "rtgtools.FN.csv:md5,80f23e0271742bc7400804311dd3b437", - "rtgtools.FP.csv:md5,c5229b5b7fd78d90e9a54e64885728e5", - "rtgtools.TP_base.csv:md5,aae4d65a540533faa4f275d0f7e9753a", - "rtgtools.TP_comp.csv:md5,b2d6fa254026b506ef64ccf1f12ab21a", "test1.HG002.strelka.extended.csv:md5,098dc352d1beeab27e72f97e64fc182e", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,bd1f1a1e138f75511de7aae157fa2fa4", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8912022beb3c5b234d29300b764b56bc", @@ -595,6 +575,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:11:26.258107754" + "timestamp": "2024-11-22T13:13:38.90109915" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index f3dacf1..fc7dce1 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -325,10 +325,6 @@ "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", - "rtgtools.FN.csv:md5,fb46816d5d479287984c4005fdd1631a", - "rtgtools.FP.csv:md5,24761919bc77724a3e1582e3bbee01cb", - "rtgtools.TP_base.csv:md5,ed42cd3e248812c1a3e1cf4985b92539", - "rtgtools.TP_comp.csv:md5,49259e81f11d9014c4ccea064a1a05b1", "test1.HG002.strelka.extended.csv:md5,fadf06276179c1cc388007e5651fb9af", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a08665be8ea20855a14d6418d07521dc", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8d4a20a5914379230952aeb252845e2d", @@ -355,7 +351,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:25:06.723892719" + "timestamp": "2024-11-22T13:34:14.298465035" }, "-stub": { "content": [ diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index e4936e4..6107271 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -169,11 +169,7 @@ ], [ "SEQC2.bcftools_stats.txt:md5,e530daf4f6a4923f1cd85c51893d5747", - "test1.SEQC2.freebayes.features.csv:md5,41752d01679b1efee789bdf0573b6057", - "test1.SEQC2.freebayes.stats.csv:md5,b2c32225c192f4704f6ce16dfb64dacb", "test1.freebayes.bcftools_stats.txt:md5,c5025b5c6256c1d808badee73a7c220c", - "test2.SEQC2.strelka.features.csv:md5,c5af06f0e6d85e53f890826b86d2d0ec", - "test2.SEQC2.strelka.stats.csv:md5,dbf9f81ef2d2c92c70f845bf0ed21929", "test2.strelka.bcftools_stats.txt:md5,ec553881bc70c786e8cec95192823634" ] ], @@ -181,6 +177,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:27:18.206645077" + "timestamp": "2024-11-22T12:53:42.07320983" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index e71e9d7..4c4cccb 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -118,14 +118,8 @@ ], [ "SEQC2.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.SEQC2.freebayes.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.SEQC2.freebayes.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.freebayes.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.SEQC2.manta.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.SEQC2.manta.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.SEQC2.strelka.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.SEQC2.strelka.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.strelka.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], @@ -133,6 +127,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T11:08:48.302568675" + "timestamp": "2024-11-22T12:57:55.883023408" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index a6dbdde..dba5048 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -189,10 +189,6 @@ [ "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", "SEQC2_mqc.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", - "truvari.FN.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", - "truvari.FP.csv:md5,d47360e5f1260a7d1cfa1797a7814ef8", - "truvari.TP_base.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", - "truvari.TP_comp.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", "test1.tiddit_mqc.stats:md5,0eb749429a6072f6bf63d377e37514d1", "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", @@ -203,6 +199,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T10:35:32.636046624" + "timestamp": "2024-11-22T13:00:22.984612013" } } \ No newline at end of file From 0fb9c97dd0837ba94c0c23f3000a641424c135a5 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 25 Nov 2024 09:21:31 +0000 Subject: [PATCH 208/292] change oras with https for singularity --- modules.json | 2 +- modules/local/variant_extractor/main.nf | 2 +- modules/nf-core/datavzrd/main.nf | 4 ++-- 3 files changed, 4 insertions(+), 4 deletions(-) diff --git a/modules.json b/modules.json index cfd26cd..5924b6e 100644 --- a/modules.json +++ b/modules.json @@ -57,7 +57,7 @@ }, "datavzrd": { "branch": "master", - "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "git_sha": "09f36ce2d721684f2883fdde4353c563ea15a48c", "installed_by": ["modules"] }, "happy/happy": { diff --git a/modules/local/variant_extractor/main.nf b/modules/local/variant_extractor/main.nf index b2bb932..25e9ec5 100644 --- a/modules/local/variant_extractor/main.nf +++ b/modules/local/variant_extractor/main.nf @@ -4,7 +4,7 @@ process VARIANT_EXTRACTOR { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'oras://community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:a12ef217eccf6ba8': + 'https://community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:a12ef217eccf6ba8': 'community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:a12ef217eccf6ba8' }" input: diff --git a/modules/nf-core/datavzrd/main.nf b/modules/nf-core/datavzrd/main.nf index a8a6261..c9b6e03 100644 --- a/modules/nf-core/datavzrd/main.nf +++ b/modules/nf-core/datavzrd/main.nf @@ -4,8 +4,8 @@ process DATAVZRD { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'oras://community.wave.seqera.io/library/datavzrd:2.36.12--bb93c8c988b7a9af': - 'community.wave.seqera.io/library/datavzrd:2.36.12--593eb75e566b7f2a' }" + 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c4/c44b3faa30ec68edb0ca92766fb49bd8f526d56c1a034d1acdb0d1448b42adec/data': + 'community.wave.seqera.io/library/datavzrd:2.36.12--dcdc5d4c72e652e2' }" input: tuple val(meta), file(config_file), file(table) From 0262907810a806064a0ac2f62c1253980a88a606 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 25 Nov 2024 10:18:13 +0000 Subject: [PATCH 209/292] renew snapshots --- tests/germline_small.nf.test.snap | 34 +++++++++++++---------- tests/germline_sv.nf.test.snap | 46 +++++++++++++++++++------------ tests/liftover_hg38.nf.test.snap | 17 +++++++----- tests/somatic_sv.nf.test.snap | 17 +++++++----- 4 files changed, 68 insertions(+), 46 deletions(-) diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 500de1a..7ed076a 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -77,11 +77,10 @@ "small/multiqc/multiqc_report.html", "small/summary", "small/summary/comparisons", - "small/summary/comparisons/small", - "small/summary/comparisons/small/rtgtools.FN.csv", - "small/summary/comparisons/small/rtgtools.FP.csv", - "small/summary/comparisons/small/rtgtools.TP_base.csv", - "small/summary/comparisons/small/rtgtools.TP_comp.csv", + "small/summary/comparisons/rtgtools.FN.csv", + "small/summary/comparisons/rtgtools.FP.csv", + "small/summary/comparisons/rtgtools.TP_base.csv", + "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", "small/summary/datavzrd/happy", "small/summary/datavzrd/happy/index.html", @@ -207,6 +206,10 @@ "genome.sdf:md5,d41d8cd98f00b204e9800998ecf8427e", "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "rtgtools.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -243,9 +246,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:10:22.614364432" + "timestamp": "2024-11-25T09:48:09.510907471" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -350,11 +353,10 @@ "small/multiqc/multiqc_report.html", "small/summary", "small/summary/comparisons", - "small/summary/comparisons/small", - "small/summary/comparisons/small/rtgtools.FN.csv", - "small/summary/comparisons/small/rtgtools.FP.csv", - "small/summary/comparisons/small/rtgtools.TP_base.csv", - "small/summary/comparisons/small/rtgtools.TP_comp.csv", + "small/summary/comparisons/rtgtools.FN.csv", + "small/summary/comparisons/rtgtools.FP.csv", + "small/summary/comparisons/rtgtools.TP_base.csv", + "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", "small/summary/datavzrd/happy", "small/summary/datavzrd/happy/index.html", @@ -524,6 +526,10 @@ "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.bcftools_stats.txt:md5,b215fc0030c53bc8887e28b23b97efb6", + "rtgtools.FN.csv:md5,80f23e0271742bc7400804311dd3b437", + "rtgtools.FP.csv:md5,c5229b5b7fd78d90e9a54e64885728e5", + "rtgtools.TP_base.csv:md5,aae4d65a540533faa4f275d0f7e9753a", + "rtgtools.TP_comp.csv:md5,b2d6fa254026b506ef64ccf1f12ab21a", "test1.HG002.strelka.extended.csv:md5,098dc352d1beeab27e72f97e64fc182e", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,bd1f1a1e138f75511de7aae157fa2fa4", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8912022beb3c5b234d29300b764b56bc", @@ -548,8 +554,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-13T08:41:21.414009313" + "timestamp": "2024-11-25T09:46:05.192857038" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index e845a97..dab2be8 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -92,13 +92,12 @@ "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", - "structural/summary/comparisons/structural", - "structural/summary/comparisons/structural/svbenchmark.FN.csv", - "structural/summary/comparisons/structural/svbenchmark.FP.csv", - "structural/summary/comparisons/structural/truvari.FN.csv", - "structural/summary/comparisons/structural/truvari.FP.csv", - "structural/summary/comparisons/structural/truvari.TP_base.csv", - "structural/summary/comparisons/structural/truvari.TP_comp.csv", + "structural/summary/comparisons/svbenchmark.FN.csv", + "structural/summary/comparisons/svbenchmark.FP.csv", + "structural/summary/comparisons/truvari.FN.csv", + "structural/summary/comparisons/truvari.FP.csv", + "structural/summary/comparisons/truvari.TP_base.csv", + "structural/summary/comparisons/truvari.TP_comp.csv", "structural/summary/datavzrd", "structural/summary/datavzrd/svbenchmark", "structural/summary/datavzrd/svbenchmark/index.html", @@ -298,6 +297,12 @@ [ "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", + "svbenchmark.FN.csv:md5,fda320e4b95452acedfdc8cc3a472ad3", + "svbenchmark.FP.csv:md5,35068a0c16903cba32257a8d9a6d51a2", + "truvari.FN.csv:md5,7d2992f331488dd307831a25b8157b0a", + "truvari.FP.csv:md5,cdc258e9153f7a75fc8340ce7211b577", + "truvari.TP_base.csv:md5,cd6d6a9c870739378c7e0ecc6868ebf6", + "truvari.TP_comp.csv:md5,4a47608038a1301cb518d872619245ff", "test1.HG002.manta.distances:md5,33dcbe29f7129dc30c184b564803ec63", "test1.HG002.manta.report:md5,91b0fbfd6fcdf45b85290f92c5a470fb", "test1.manta.bcftools_stats.txt:md5,0e4cf1707b245c026fe6a0af6e8f8c0a", @@ -314,9 +319,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T15:58:48.350235224" + "timestamp": "2024-11-25T09:51:11.885074416" }, "-stub": { "content": [ @@ -407,13 +412,12 @@ "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", - "structural/summary/comparisons/structural", - "structural/summary/comparisons/structural/svbenchmark.FN.csv", - "structural/summary/comparisons/structural/svbenchmark.FP.csv", - "structural/summary/comparisons/structural/truvari.FN.csv", - "structural/summary/comparisons/structural/truvari.FP.csv", - "structural/summary/comparisons/structural/truvari.TP_base.csv", - "structural/summary/comparisons/structural/truvari.TP_comp.csv", + "structural/summary/comparisons/svbenchmark.FN.csv", + "structural/summary/comparisons/svbenchmark.FP.csv", + "structural/summary/comparisons/truvari.FN.csv", + "structural/summary/comparisons/truvari.FP.csv", + "structural/summary/comparisons/truvari.TP_base.csv", + "structural/summary/comparisons/truvari.TP_comp.csv", "structural/summary/datavzrd", "structural/summary/datavzrd/svbenchmark", "structural/summary/datavzrd/svbenchmark/index.html", @@ -593,6 +597,12 @@ "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "HG002.stats:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", + "svbenchmark.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "svbenchmark.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", + "truvari.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -627,8 +637,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:01:22.753474251" + "timestamp": "2024-11-25T09:53:34.16731435" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index bde2593..ed09589 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -113,11 +113,10 @@ "small/multiqc/multiqc_report.html", "small/summary", "small/summary/comparisons", - "small/summary/comparisons/small", - "small/summary/comparisons/small/rtgtools.FN.csv", - "small/summary/comparisons/small/rtgtools.FP.csv", - "small/summary/comparisons/small/rtgtools.TP_base.csv", - "small/summary/comparisons/small/rtgtools.TP_comp.csv", + "small/summary/comparisons/rtgtools.FN.csv", + "small/summary/comparisons/rtgtools.FP.csv", + "small/summary/comparisons/rtgtools.TP_base.csv", + "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", "small/summary/datavzrd/happy", "small/summary/datavzrd/happy/index.html", @@ -286,6 +285,10 @@ "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", + "rtgtools.FN.csv:md5,7690798baa198e924c08e03e301148d4", + "rtgtools.FP.csv:md5,35f34c2f3b37f9c4a0617d450decee92", + "rtgtools.TP_base.csv:md5,344d3777b90a06e73aa59d1cc050015a", + "rtgtools.TP_comp.csv:md5,9ed56d1b7e88177723ad7b5bf584bc43", "test1.HG002.strelka.extended.csv:md5,fadf06276179c1cc388007e5651fb9af", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a08665be8ea20855a14d6418d07521dc", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8d4a20a5914379230952aeb252845e2d", @@ -310,9 +313,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-13T09:00:38.410760165" + "timestamp": "2024-11-25T10:00:28.23191397" }, "-stub": { "content": [ diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index 331d865..2cc29ba 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -78,11 +78,10 @@ "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", - "structural/summary/comparisons/structural", - "structural/summary/comparisons/structural/truvari.FN.csv", - "structural/summary/comparisons/structural/truvari.FP.csv", - "structural/summary/comparisons/structural/truvari.TP_base.csv", - "structural/summary/comparisons/structural/truvari.TP_comp.csv", + "structural/summary/comparisons/truvari.FN.csv", + "structural/summary/comparisons/truvari.FP.csv", + "structural/summary/comparisons/truvari.TP_base.csv", + "structural/summary/comparisons/truvari.TP_comp.csv", "structural/summary/datavzrd", "structural/summary/datavzrd/truvari", "structural/summary/datavzrd/truvari/index.html", @@ -161,6 +160,10 @@ [ "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", "SEQC2.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", + "truvari.FN.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", + "truvari.FP.csv:md5,d47360e5f1260a7d1cfa1797a7814ef8", + "truvari.TP_base.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", + "truvari.TP_comp.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", "test1.tiddit.stats:md5,0eb749429a6072f6bf63d377e37514d1", "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", @@ -169,8 +172,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:27:02.901069711" + "timestamp": "2024-11-25T10:08:50.703058874" } } \ No newline at end of file From 2d73bcec8d6971df60ca3f0f834174838c0d63e9 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 25 Nov 2024 10:58:06 +0000 Subject: [PATCH 210/292] renew tests --- tests/.nftignore | 17 +++++++++++----- tests/germline_small.nf.test.snap | 24 ++-------------------- tests/germline_sv.nf.test.snap | 34 ++----------------------------- tests/liftover_hg38.nf.test.snap | 6 +----- tests/somatic_indel.nf.test.snap | 8 ++------ tests/somatic_snv.nf.test.snap | 16 ++------------- tests/somatic_sv.nf.test.snap | 6 +----- 7 files changed, 22 insertions(+), 89 deletions(-) diff --git a/tests/.nftignore b/tests/.nftignore index 148cde2..ee8bdcf 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -9,17 +9,24 @@ references/rtgtools/genome.sdf/progress references/rtgtools/genome.sdf/format.log references/rtgtools/genome.sdf/mainIndex references/rtgtools/genome.sdf/done -**/summary/tables/**/*.csv **/summary/datavzrd/versions.yml **/summary/datavzrd/**/index.html **/summary/datavzrd/**/static/* -**/summary/datavzrd/**/test/*.js +**/summary/datavzrd/**/static/*.{js} +**/summary/datavzrd/**/static/*.{css} +**/summary/datavzrd/**/test/*.{js} **/summary/datavzrd/**/test/index_1.html **/summary/datavzrd/**/test/data/data_1.js -**/summary/datavzrd/**/test/plots/*.js +**/summary/datavzrd/**/test/plots/*.{js} +**/summary/datavzrd/**/network/plots/*.{js} +**/summary/datavzrd/**/network/*.{js} +**/summary/datavzrd/**/network/index_1.html +**/summary/datavzrd/**/network/data/data_1.js **/summary/plots/**/*.png -**/summary/comparisons/**/*.csv +**/summary/comparisons/*.csv +**/summary/tables/**/*.csv **/**/benchmarks/**/*.{json,json.gz,tsv.gz,vcf.gz,gz.tbi} -**/**/benchmarks/svanalyzer/*.{log} +**/**/benchmarks/svanalyzer/*.log +**/**/benchmarks/sompy/*.csv **/**/preprocess/*.{vcf.gz,gz.tbi,vcf} **/**/liftover/*.{vcf.gz,gz.tbi,vcf} diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 7ed076a..2c0280e 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -206,22 +206,6 @@ "genome.sdf:md5,d41d8cd98f00b204e9800998ecf8427e", "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "rtgtools.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.strelka.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", @@ -248,7 +232,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-25T09:48:09.510907471" + "timestamp": "2024-11-25T10:39:44.71491264" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -526,10 +510,6 @@ "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.bcftools_stats.txt:md5,b215fc0030c53bc8887e28b23b97efb6", - "rtgtools.FN.csv:md5,80f23e0271742bc7400804311dd3b437", - "rtgtools.FP.csv:md5,c5229b5b7fd78d90e9a54e64885728e5", - "rtgtools.TP_base.csv:md5,aae4d65a540533faa4f275d0f7e9753a", - "rtgtools.TP_comp.csv:md5,b2d6fa254026b506ef64ccf1f12ab21a", "test1.HG002.strelka.extended.csv:md5,098dc352d1beeab27e72f97e64fc182e", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,bd1f1a1e138f75511de7aae157fa2fa4", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8912022beb3c5b234d29300b764b56bc", @@ -556,6 +536,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-25T09:46:05.192857038" + "timestamp": "2024-11-25T10:37:40.360294798" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index dab2be8..c851389 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -297,12 +297,6 @@ [ "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", "HG002.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "svbenchmark.FN.csv:md5,fda320e4b95452acedfdc8cc3a472ad3", - "svbenchmark.FP.csv:md5,35068a0c16903cba32257a8d9a6d51a2", - "truvari.FN.csv:md5,7d2992f331488dd307831a25b8157b0a", - "truvari.FP.csv:md5,cdc258e9153f7a75fc8340ce7211b577", - "truvari.TP_base.csv:md5,cd6d6a9c870739378c7e0ecc6868ebf6", - "truvari.TP_comp.csv:md5,4a47608038a1301cb518d872619245ff", "test1.HG002.manta.distances:md5,33dcbe29f7129dc30c184b564803ec63", "test1.HG002.manta.report:md5,91b0fbfd6fcdf45b85290f92c5a470fb", "test1.manta.bcftools_stats.txt:md5,0e4cf1707b245c026fe6a0af6e8f8c0a", @@ -321,7 +315,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-25T09:51:11.885074416" + "timestamp": "2024-11-25T10:42:37.100999399" }, "-stub": { "content": [ @@ -597,30 +591,6 @@ "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "HG002.stats:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", - "svbenchmark.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "svbenchmark.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.FN.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.FP.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.TP_base.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "truvari.TP_comp.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.manta.distances:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.HG002.manta.report:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -639,6 +609,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-25T09:53:34.16731435" + "timestamp": "2024-11-25T10:44:55.497510148" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index ed09589..0d00c0f 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -285,10 +285,6 @@ "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", - "rtgtools.FN.csv:md5,7690798baa198e924c08e03e301148d4", - "rtgtools.FP.csv:md5,35f34c2f3b37f9c4a0617d450decee92", - "rtgtools.TP_base.csv:md5,344d3777b90a06e73aa59d1cc050015a", - "rtgtools.TP_comp.csv:md5,9ed56d1b7e88177723ad7b5bf584bc43", "test1.HG002.strelka.extended.csv:md5,fadf06276179c1cc388007e5651fb9af", "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a08665be8ea20855a14d6418d07521dc", "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8d4a20a5914379230952aeb252845e2d", @@ -315,7 +311,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-25T10:00:28.23191397" + "timestamp": "2024-11-25T10:49:53.896332791" }, "-stub": { "content": [ diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 7376835..027d594 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -129,18 +129,14 @@ ], [ "SEQC2.bcftools_stats.txt:md5,e530daf4f6a4923f1cd85c51893d5747", - "test1.SEQC2.freebayes.features.csv:md5,41752d01679b1efee789bdf0573b6057", - "test1.SEQC2.freebayes.stats.csv:md5,b2c32225c192f4704f6ce16dfb64dacb", "test1.freebayes.bcftools_stats.txt:md5,c5025b5c6256c1d808badee73a7c220c", - "test2.SEQC2.strelka.features.csv:md5,c5af06f0e6d85e53f890826b86d2d0ec", - "test2.SEQC2.strelka.stats.csv:md5,dbf9f81ef2d2c92c70f845bf0ed21929", "test2.strelka.bcftools_stats.txt:md5,ec553881bc70c786e8cec95192823634" ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:14:37.608031284" + "timestamp": "2024-11-25T10:51:58.00620306" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 278af4a..2d90453 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -119,27 +119,15 @@ [ "SEQC2.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.SEQC2.freebayes.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.SEQC2.freebayes.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.freebayes.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.SEQC2.manta.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.SEQC2.manta.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test2.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.SEQC2.strelka.features.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test3.SEQC2.strelka.stats.csv:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.strelka.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e" ] ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.0" + "nextflow": "24.10.1" }, - "timestamp": "2024-11-12T16:24:28.108507744" + "timestamp": "2024-11-25T10:55:52.928254209" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index 2cc29ba..54572a7 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -160,10 +160,6 @@ [ "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", "SEQC2.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", - "truvari.FN.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", - "truvari.FP.csv:md5,d47360e5f1260a7d1cfa1797a7814ef8", - "truvari.TP_base.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", - "truvari.TP_comp.csv:md5,ec8c0aa9227f6e578136e2e53c15a097", "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", "test1.tiddit.stats:md5,0eb749429a6072f6bf63d377e37514d1", "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", @@ -174,6 +170,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-25T10:08:50.703058874" + "timestamp": "2024-11-25T10:57:54.814250778" } } \ No newline at end of file From 4bdd9a4028ed6725d1e7e1119c9e0634619b3a13 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 26 Nov 2024 11:47:53 +0000 Subject: [PATCH 211/292] fix multiqc config --- assets/multiqc_config.yml | 11 ++--------- workflows/variantbenchmarking.nf | 2 +- 2 files changed, 3 insertions(+), 10 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 1ec952a..6b654de 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -17,6 +17,7 @@ ignore_images: false # Run only these modules run_modules: - bcftools + - custom_content custom_data: survivor: @@ -29,15 +30,7 @@ custom_data: id: "survivor" namespace: "SURVIVOR variant statistics" table_title: "Variant statistics from survivor stats tool" - ylab: "Len" - headers: - - "Len" - - "Del" - - "INS" - - "Dup" - - "Inv" - - "TRA" - - "UNK" + ylab: "Count" summary_reports: id: "summary_reports" diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 9cec93b..0cd825e 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -254,7 +254,7 @@ workflow VARIANTBENCHMARKING { ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml',sort: true)) ch_multiqc_files = ch_multiqc_files.mix(REPORT_BENCHMARK_STATISTICS.out.ch_plots) - ch_multiqc_files.view() + MULTIQC ( ch_multiqc_files.collect(), ch_multiqc_config.toList(), From f21aa64675995a6e74f542e18dec0aeb438a3ba0 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 26 Nov 2024 14:04:19 +0000 Subject: [PATCH 212/292] renew snopshots --- tests/.nftignore | 4 +++- tests/germline_sv.nf.test.snap | 9 ++++++++- tests/somatic_sv.nf.test.snap | 9 ++++++++- 3 files changed, 19 insertions(+), 3 deletions(-) diff --git a/tests/.nftignore b/tests/.nftignore index ee8bdcf..1b219f2 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -1,6 +1,8 @@ **/*_report.html **/multiqc/multiqc_data/* -**/multiqc/multiqc_plots/{svg,pdf,png}/*.{svg,pdf,png} +**/multiqc/multiqc_plots/svg/*.{svg,pdf,png} +**/multiqc/multiqc_plots/png/*.{svg,pdf,png} +**/multiqc/multiqc_plots/pdf/*.{svg,pdf,png} **/multiqc/multiqc_report.html pipeline_info/*.{html,json,txt,yml} references/dictionary/genome.dict diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index b04185a..ecb99af 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -102,6 +102,7 @@ "structural/multiqc/multiqc_data/multiqc_general_stats.txt", "structural/multiqc/multiqc_data/multiqc_software_versions.txt", "structural/multiqc/multiqc_data/multiqc_sources.txt", + "structural/multiqc/multiqc_data/multiqc_survivor.txt", "structural/multiqc/multiqc_plots", "structural/multiqc/multiqc_plots/pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", @@ -110,6 +111,8 @@ "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", "structural/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "structural/multiqc/multiqc_plots/pdf/survivor-cnt.pdf", + "structural/multiqc/multiqc_plots/pdf/survivor-pct.pdf", "structural/multiqc/multiqc_plots/png", "structural/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", @@ -117,6 +120,8 @@ "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", "structural/multiqc/multiqc_plots/png/general_stats_table.png", + "structural/multiqc/multiqc_plots/png/survivor-cnt.png", + "structural/multiqc/multiqc_plots/png/survivor-pct.png", "structural/multiqc/multiqc_plots/svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", @@ -124,6 +129,8 @@ "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", "structural/multiqc/multiqc_plots/svg/general_stats_table.svg", + "structural/multiqc/multiqc_plots/svg/survivor-cnt.svg", + "structural/multiqc/multiqc_plots/svg/survivor-pct.svg", "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", @@ -350,7 +357,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T12:36:40.054294704" + "timestamp": "2024-11-26T14:01:47.145700457" }, "-stub": { "content": [ diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index dba5048..81eb1c4 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -82,6 +82,7 @@ "structural/multiqc/multiqc_data/multiqc_general_stats.txt", "structural/multiqc/multiqc_data/multiqc_software_versions.txt", "structural/multiqc/multiqc_data/multiqc_sources.txt", + "structural/multiqc/multiqc_data/multiqc_survivor.txt", "structural/multiqc/multiqc_plots", "structural/multiqc/multiqc_plots/pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", @@ -90,6 +91,8 @@ "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", "structural/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "structural/multiqc/multiqc_plots/pdf/survivor-cnt.pdf", + "structural/multiqc/multiqc_plots/pdf/survivor-pct.pdf", "structural/multiqc/multiqc_plots/png", "structural/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", @@ -97,6 +100,8 @@ "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", "structural/multiqc/multiqc_plots/png/general_stats_table.png", + "structural/multiqc/multiqc_plots/png/survivor-cnt.png", + "structural/multiqc/multiqc_plots/png/survivor-pct.png", "structural/multiqc/multiqc_plots/svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", @@ -104,6 +109,8 @@ "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", "structural/multiqc/multiqc_plots/svg/general_stats_table.svg", + "structural/multiqc/multiqc_plots/svg/survivor-cnt.svg", + "structural/multiqc/multiqc_plots/svg/survivor-pct.svg", "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", @@ -199,6 +206,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.1" }, - "timestamp": "2024-11-22T13:00:22.984612013" + "timestamp": "2024-11-26T13:55:27.662287528" } } \ No newline at end of file From aba85d59ffda28169ac2e793f21b4c8742fc5550 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 28 Nov 2024 16:02:47 +0000 Subject: [PATCH 213/292] create new singularity containers for https --- modules/local/create_datavzrd_input/main.nf | 5 +++++ modules/local/variant_extractor/main.nf | 4 ++-- modules/local/vcf_to_csv/main.nf | 4 ++-- 3 files changed, 9 insertions(+), 4 deletions(-) diff --git a/modules/local/create_datavzrd_input/main.nf b/modules/local/create_datavzrd_input/main.nf index 1042dc4..5bf39e3 100644 --- a/modules/local/create_datavzrd_input/main.nf +++ b/modules/local/create_datavzrd_input/main.nf @@ -2,6 +2,11 @@ process CREATE_DATAVZRD_INPUT { tag "$meta.id" label 'process_single' + conda "conda-forge::tar=1.34" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'quay.io/nf-core/ubuntu:20.04' }" + input: tuple val(meta), path(csv), path(template) diff --git a/modules/local/variant_extractor/main.nf b/modules/local/variant_extractor/main.nf index 25e9ec5..560673c 100644 --- a/modules/local/variant_extractor/main.nf +++ b/modules/local/variant_extractor/main.nf @@ -4,8 +4,8 @@ process VARIANT_EXTRACTOR { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:a12ef217eccf6ba8': - 'community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:a12ef217eccf6ba8' }" + 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c9/c9964f2ad344f5df543a390ddceb308af0816bed9c9abb9cb400e65525580694/data': + 'community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:9a7098708edb08d4' }" input: tuple val(meta), path(input) diff --git a/modules/local/vcf_to_csv/main.nf b/modules/local/vcf_to_csv/main.nf index f17e7a4..a8abf07 100644 --- a/modules/local/vcf_to_csv/main.nf +++ b/modules/local/vcf_to_csv/main.nf @@ -4,8 +4,8 @@ process VCF_TO_CSV { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'oras://community.wave.seqera.io/library/pysam_pandas_pip_variant-extractor:a2d9ec4a4efadbc3': - 'community.wave.seqera.io/library/pysam_pandas_pip_variant-extractor:a2d9ec4a4efadbc3' }" + 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/30/30151e7153f1f845e2723b29048a3cdf842e2dcb1384cfcc86ced0211d5e4895/data': + 'community.wave.seqera.io/library/pysam_pandas_pip_variant-extractor:2818354e40f5f895' }" input: tuple val(meta), path(input) From 487da9e7c8819ea16e800a28a73b66cb0ca47858 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 29 Nov 2024 08:33:27 +0000 Subject: [PATCH 214/292] renew snapshots --- modules/local/variant_extractor/main.nf | 2 +- modules/local/vcf_to_csv/main.nf | 2 +- tests/germline_small.nf.test.snap | 8 +- tests/germline_sv.nf.test.snap | 8 +- tests/liftover_hg38.nf.test.snap | 107 +----------------------- tests/somatic_sv.nf.test.snap | 4 +- 6 files changed, 15 insertions(+), 116 deletions(-) diff --git a/modules/local/variant_extractor/main.nf b/modules/local/variant_extractor/main.nf index 560673c..b4ab1e2 100644 --- a/modules/local/variant_extractor/main.nf +++ b/modules/local/variant_extractor/main.nf @@ -5,7 +5,7 @@ process VARIANT_EXTRACTOR { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/c9/c9964f2ad344f5df543a390ddceb308af0816bed9c9abb9cb400e65525580694/data': - 'community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:9a7098708edb08d4' }" + 'community.wave.seqera.io/library/htslib_pysam_tabix_pip_variant-extractor:a12ef217eccf6ba8' }" input: tuple val(meta), path(input) diff --git a/modules/local/vcf_to_csv/main.nf b/modules/local/vcf_to_csv/main.nf index a8abf07..464581a 100644 --- a/modules/local/vcf_to_csv/main.nf +++ b/modules/local/vcf_to_csv/main.nf @@ -5,7 +5,7 @@ process VCF_TO_CSV { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/30/30151e7153f1f845e2723b29048a3cdf842e2dcb1384cfcc86ced0211d5e4895/data': - 'community.wave.seqera.io/library/pysam_pandas_pip_variant-extractor:2818354e40f5f895' }" + 'community.wave.seqera.io/library/pysam_pandas_pip_variant-extractor:a2d9ec4a4efadbc3' }" input: tuple val(meta), path(input) diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 019291b..a99138b 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -229,9 +229,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-22T13:15:43.288411916" + "timestamp": "2024-11-28T16:47:47.024615694" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -573,8 +573,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-22T13:13:38.90109915" + "timestamp": "2024-11-28T16:45:26.990755841" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index ecb99af..b566380 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -355,9 +355,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-26T14:01:47.145700457" + "timestamp": "2024-11-28T16:51:57.386421371" }, "-stub": { "content": [ @@ -654,8 +654,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-22T12:38:58.811950484" + "timestamp": "2024-11-28T16:54:34.483191183" } } \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index fc7dce1..aa0db5f 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover": { "content": [ - 85, + 75, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -57,9 +57,6 @@ "UCSC_LIFTOVER": { "ucsc": 377 }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, "Workflow": { "nf-core/variantbenchmarking": "v1.0dev" } @@ -103,97 +100,8 @@ "small/HG002/stats", "small/HG002/stats/bcftools", "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", - "small/multiqc", - "small/multiqc/multiqc_data", - "small/multiqc/multiqc_data/bcftools-stats-subtypes.txt", - "small/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", - "small/multiqc/multiqc_data/bcftools_stats_variant_depths.txt", - "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", - "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", - "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", - "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", - "small/multiqc/multiqc_data/multiqc.log", - "small/multiqc/multiqc_data/multiqc_bcftools_stats.txt", - "small/multiqc/multiqc_data/multiqc_citations.txt", - "small/multiqc/multiqc_data/multiqc_data.json", - "small/multiqc/multiqc_data/multiqc_general_stats.txt", - "small/multiqc/multiqc_data/multiqc_software_versions.txt", - "small/multiqc/multiqc_data/multiqc_sources.txt", - "small/multiqc/multiqc_plots", - "small/multiqc/multiqc_plots/pdf", - "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf", - "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf", - "small/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", - "small/multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf", - "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", - "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", - "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", - "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", - "small/multiqc/multiqc_plots/pdf/general_stats_table.pdf", - "small/multiqc/multiqc_plots/png", - "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png", - "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png", - "small/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", - "small/multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png", - "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", - "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", - "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", - "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", - "small/multiqc/multiqc_plots/png/general_stats_table.png", - "small/multiqc/multiqc_plots/svg", - "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg", - "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg", - "small/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", - "small/multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg", - "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", - "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", - "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", - "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", - "small/multiqc/multiqc_plots/svg/general_stats_table.svg", - "small/multiqc/multiqc_report.html", "small/summary", - "small/summary/comparisons", - "small/summary/comparisons/rtgtools.FN.csv", - "small/summary/comparisons/rtgtools.FP.csv", - "small/summary/comparisons/rtgtools.TP_base.csv", - "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", - "small/summary/datavzrd/happy", - "small/summary/datavzrd/happy/index.html", - "small/summary/datavzrd/happy/static", - "small/summary/datavzrd/happy/static/bootstrap-select.min.css", - "small/summary/datavzrd/happy/static/bootstrap-table-fixed-columns.min.css", - "small/summary/datavzrd/happy/static/bootstrap-table.min.css", - "small/summary/datavzrd/happy/static/bootstrap.min.css", - "small/summary/datavzrd/happy/static/bundle.js", - "small/summary/datavzrd/happy/static/datavzrd.css", - "small/summary/datavzrd/happy/test", - "small/summary/datavzrd/happy/test/config.js", - "small/summary/datavzrd/happy/test/data", - "small/summary/datavzrd/happy/test/data/data_1.js", - "small/summary/datavzrd/happy/test/functions.js", - "small/summary/datavzrd/happy/test/heatmap.js", - "small/summary/datavzrd/happy/test/index_1.html", - "small/summary/datavzrd/happy/test/plots", - "small/summary/datavzrd/happy/test/plots/plot_0.js", - "small/summary/datavzrd/happy/test/plots/plot_1.js", - "small/summary/datavzrd/happy/test/plots/plot_10.js", - "small/summary/datavzrd/happy/test/plots/plot_11.js", - "small/summary/datavzrd/happy/test/plots/plot_12.js", - "small/summary/datavzrd/happy/test/plots/plot_13.js", - "small/summary/datavzrd/happy/test/plots/plot_14.js", - "small/summary/datavzrd/happy/test/plots/plot_15.js", - "small/summary/datavzrd/happy/test/plots/plot_16.js", - "small/summary/datavzrd/happy/test/plots/plot_17.js", - "small/summary/datavzrd/happy/test/plots/plot_18.js", - "small/summary/datavzrd/happy/test/plots/plot_2.js", - "small/summary/datavzrd/happy/test/plots/plot_3.js", - "small/summary/datavzrd/happy/test/plots/plot_4.js", - "small/summary/datavzrd/happy/test/plots/plot_5.js", - "small/summary/datavzrd/happy/test/plots/plot_6.js", - "small/summary/datavzrd/happy/test/plots/plot_7.js", - "small/summary/datavzrd/happy/test/plots/plot_8.js", - "small/summary/datavzrd/happy/test/plots/plot_9.js", "small/summary/datavzrd/rtgtools", "small/summary/datavzrd/rtgtools/index.html", "small/summary/datavzrd/rtgtools/static", @@ -222,15 +130,6 @@ "small/summary/datavzrd/rtgtools/test/plots/plot_8.js", "small/summary/datavzrd/versions.yml", "small/summary/plots", - "small/summary/plots/happy", - "small/summary/plots/happy/INDEL_ALL_metric_by_tool_happy_mqc.png", - "small/summary/plots/happy/INDEL_ALL_variants_by_tool_happy_mqc.png", - "small/summary/plots/happy/INDEL_PASS_metric_by_tool_happy_mqc.png", - "small/summary/plots/happy/INDEL_PASS_variants_by_tool_happy_mqc.png", - "small/summary/plots/happy/SNP_ALL_metric_by_tool_happy_mqc.png", - "small/summary/plots/happy/SNP_ALL_variants_by_tool_happy_mqc.png", - "small/summary/plots/happy/SNP_PASS_metric_by_tool_happy_mqc.png", - "small/summary/plots/happy/SNP_PASS_variants_by_tool_happy_mqc.png", "small/summary/plots/rtgtools", "small/summary/plots/rtgtools/metric_by_tool_rtgtools_mqc.png", "small/summary/plots/rtgtools/variants_by_tool_rtgtools_mqc.png", @@ -349,9 +248,9 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-22T13:34:14.298465035" + "timestamp": "2024-11-28T16:26:36.118564689" }, "-stub": { "content": [ diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index 81eb1c4..fabf609 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -204,8 +204,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-26T13:55:27.662287528" + "timestamp": "2024-11-28T16:58:12.671419675" } } \ No newline at end of file From 786b95c0b6a42e081de476c9ab843fa0dbc9daab Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 29 Nov 2024 09:05:31 +0000 Subject: [PATCH 215/292] supress happy error --- tests/.nftignore | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/tests/.nftignore b/tests/.nftignore index 1b219f2..726ae37 100644 --- a/tests/.nftignore +++ b/tests/.nftignore @@ -19,11 +19,13 @@ references/rtgtools/genome.sdf/done **/summary/datavzrd/**/test/*.{js} **/summary/datavzrd/**/test/index_1.html **/summary/datavzrd/**/test/data/data_1.js -**/summary/datavzrd/**/test/plots/*.{js} -**/summary/datavzrd/**/network/plots/*.{js} +**/summary/datavzrd/**/test/plots/*.js +**/summary/datavzrd/**/network/plots/*.js **/summary/datavzrd/**/network/*.{js} **/summary/datavzrd/**/network/index_1.html **/summary/datavzrd/**/network/data/data_1.js +**/summary/plots/happy/*.png +**/summary/plots/rtgtools/*.png **/summary/plots/**/*.png **/summary/comparisons/*.csv **/summary/tables/**/*.csv From a00e84c619c251485191b2cfd8b35d1648ddfe14 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 29 Nov 2024 09:30:51 +0000 Subject: [PATCH 216/292] renew snapshot for liftover --- tests/liftover_hg38.nf.test.snap | 105 ++++++++++++++++++++++++++++++- 1 file changed, 103 insertions(+), 2 deletions(-) diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_hg38.nf.test.snap index aa0db5f..55af699 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_hg38.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover": { "content": [ - 75, + 85, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -57,6 +57,9 @@ "UCSC_LIFTOVER": { "ucsc": 377 }, + "VCF_TO_CSV": { + "python": "3.12.4" + }, "Workflow": { "nf-core/variantbenchmarking": "v1.0dev" } @@ -100,8 +103,97 @@ "small/HG002/stats", "small/HG002/stats/bcftools", "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", + "small/multiqc", + "small/multiqc/multiqc_data", + "small/multiqc/multiqc_data/bcftools-stats-subtypes.txt", + "small/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "small/multiqc/multiqc_data/bcftools_stats_variant_depths.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "small/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", + "small/multiqc/multiqc_data/multiqc.log", + "small/multiqc/multiqc_data/multiqc_bcftools_stats.txt", + "small/multiqc/multiqc_data/multiqc_citations.txt", + "small/multiqc/multiqc_data/multiqc_data.json", + "small/multiqc/multiqc_data/multiqc_general_stats.txt", + "small/multiqc/multiqc_data/multiqc_software_versions.txt", + "small/multiqc/multiqc_data/multiqc_sources.txt", + "small/multiqc/multiqc_plots", + "small/multiqc/multiqc_plots/pdf", + "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "small/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", + "small/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "small/multiqc/multiqc_plots/png", + "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png", + "small/multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "small/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", + "small/multiqc/multiqc_plots/png/general_stats_table.png", + "small/multiqc/multiqc_plots/svg", + "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg", + "small/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "small/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", + "small/multiqc/multiqc_plots/svg/general_stats_table.svg", + "small/multiqc/multiqc_report.html", "small/summary", + "small/summary/comparisons", + "small/summary/comparisons/rtgtools.FN.csv", + "small/summary/comparisons/rtgtools.FP.csv", + "small/summary/comparisons/rtgtools.TP_base.csv", + "small/summary/comparisons/rtgtools.TP_comp.csv", "small/summary/datavzrd", + "small/summary/datavzrd/happy", + "small/summary/datavzrd/happy/index.html", + "small/summary/datavzrd/happy/static", + "small/summary/datavzrd/happy/static/bootstrap-select.min.css", + "small/summary/datavzrd/happy/static/bootstrap-table-fixed-columns.min.css", + "small/summary/datavzrd/happy/static/bootstrap-table.min.css", + "small/summary/datavzrd/happy/static/bootstrap.min.css", + "small/summary/datavzrd/happy/static/bundle.js", + "small/summary/datavzrd/happy/static/datavzrd.css", + "small/summary/datavzrd/happy/test", + "small/summary/datavzrd/happy/test/config.js", + "small/summary/datavzrd/happy/test/data", + "small/summary/datavzrd/happy/test/data/data_1.js", + "small/summary/datavzrd/happy/test/functions.js", + "small/summary/datavzrd/happy/test/heatmap.js", + "small/summary/datavzrd/happy/test/index_1.html", + "small/summary/datavzrd/happy/test/plots", + "small/summary/datavzrd/happy/test/plots/plot_0.js", + "small/summary/datavzrd/happy/test/plots/plot_1.js", + "small/summary/datavzrd/happy/test/plots/plot_10.js", + "small/summary/datavzrd/happy/test/plots/plot_11.js", + "small/summary/datavzrd/happy/test/plots/plot_12.js", + "small/summary/datavzrd/happy/test/plots/plot_13.js", + "small/summary/datavzrd/happy/test/plots/plot_14.js", + "small/summary/datavzrd/happy/test/plots/plot_15.js", + "small/summary/datavzrd/happy/test/plots/plot_16.js", + "small/summary/datavzrd/happy/test/plots/plot_17.js", + "small/summary/datavzrd/happy/test/plots/plot_18.js", + "small/summary/datavzrd/happy/test/plots/plot_2.js", + "small/summary/datavzrd/happy/test/plots/plot_3.js", + "small/summary/datavzrd/happy/test/plots/plot_4.js", + "small/summary/datavzrd/happy/test/plots/plot_5.js", + "small/summary/datavzrd/happy/test/plots/plot_6.js", + "small/summary/datavzrd/happy/test/plots/plot_7.js", + "small/summary/datavzrd/happy/test/plots/plot_8.js", + "small/summary/datavzrd/happy/test/plots/plot_9.js", "small/summary/datavzrd/rtgtools", "small/summary/datavzrd/rtgtools/index.html", "small/summary/datavzrd/rtgtools/static", @@ -130,6 +222,15 @@ "small/summary/datavzrd/rtgtools/test/plots/plot_8.js", "small/summary/datavzrd/versions.yml", "small/summary/plots", + "small/summary/plots/happy", + "small/summary/plots/happy/INDEL_ALL_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_ALL_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_PASS_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/INDEL_PASS_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_ALL_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_ALL_variants_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_PASS_metric_by_tool_happy_mqc.png", + "small/summary/plots/happy/SNP_PASS_variants_by_tool_happy_mqc.png", "small/summary/plots/rtgtools", "small/summary/plots/rtgtools/metric_by_tool_rtgtools_mqc.png", "small/summary/plots/rtgtools/variants_by_tool_rtgtools_mqc.png", @@ -250,7 +351,7 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-11-28T16:26:36.118564689" + "timestamp": "2024-11-29T09:23:31.648065377" }, "-stub": { "content": [ From 823ceceb0e3b4394aa5dd531f53451e6265d0ef3 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 3 Dec 2024 11:14:30 +0000 Subject: [PATCH 217/292] remove failing truth.md links --- docs/truth.md | 2 -- docs/usage.md | 2 +- 2 files changed, 1 insertion(+), 3 deletions(-) diff --git a/docs/truth.md b/docs/truth.md index 0222fb2..cec7073 100644 --- a/docs/truth.md +++ b/docs/truth.md @@ -1,7 +1,5 @@ # nf-core/variantbenchmarking: Truth files -## :warning: Please read this documentation on the nf-core website: [https://nf-co.re/variantbenchmarking/truth](https://nf-co.re/variantbenchmarking/truth) - ## Defining Truth VCF and High confidence BED files This pipeline requires a set of Truth VCF, as a baseline for comparisons, and a high confidence bed files, to restrict analysis to regions. Although, those sets can be anything depending on the type of the analysis, for benchmarking of human genomes there are golden set of samples provided by [Genome in a Bottle project](https://www.nist.gov/programs-projects/genome-bottle) and [SEQC2 consortium](https://sites.google.com/view/seqc2/home/data-analysis/high-confidence-somatic-snv-and-indel-v1-2). diff --git a/docs/usage.md b/docs/usage.md index b7c6047..e56db2c 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -41,7 +41,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p ## Truth samples -Please find the detailed information about truth samples [here](https://nf-co.re/variantbenchmarking/truth). +Please find the detailed information about truth samples [here](/workspace/variantbenchmarking/docs/truth.md). ## Lifting over truth sets From 4c770b236b4bafa934482aecf81024895cfdbe66 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 3 Dec 2024 16:21:18 +0000 Subject: [PATCH 218/292] add liftover option for test vcfs --- assets/schema_input.json | 6 + ...tover_hg37.config => liftover_test.config} | 7 +- ...over_hg38.config => liftover_truth.config} | 2 +- nextflow.config | 6 +- nextflow_schema.json | 13 +- ...iftover_vcfs_truth.nf => liftover_vcfs.nf} | 14 +- subworkflows/local/prepare_vcfs_test.nf | 38 ++- subworkflows/local/prepare_vcfs_truth.nf | 12 +- tests/liftover_test.nf.test | 40 +++ tests/liftover_test.nf.test.snap | 208 ++++++++++++++ ...er_hg38.nf.test => liftover_truth.nf.test} | 6 +- ....test.snap => liftover_truth.nf.test.snap} | 267 +----------------- workflows/variantbenchmarking.nf | 29 +- 13 files changed, 341 insertions(+), 307 deletions(-) rename conf/tests/{liftover_hg37.config => liftover_test.config} (79%) rename conf/tests/{liftover_hg38.config => liftover_truth.config} (98%) rename subworkflows/local/{liftover_vcfs_truth.nf => liftover_vcfs.nf} (87%) create mode 100644 tests/liftover_test.nf.test create mode 100644 tests/liftover_test.nf.test.snap rename tests/{liftover_hg38.nf.test => liftover_truth.nf.test} (87%) rename tests/{liftover_hg38.nf.test.snap => liftover_truth.nf.test.snap} (59%) diff --git a/assets/schema_input.json b/assets/schema_input.json index 7967366..c6a6cf1 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -154,6 +154,12 @@ "enum": ["sc", "cts", "d"], "minLength": 1, "default": null + }, + "liftover": { + "type": "boolean", + "description": "Liftover option for test vcfs, to activate add params.liftover='test' ", + "meta": ["liftover"], + "default": false } }, "required": ["test_vcf", "caller", "id"] diff --git a/conf/tests/liftover_hg37.config b/conf/tests/liftover_test.config similarity index 79% rename from conf/tests/liftover_hg37.config rename to conf/tests/liftover_test.config index e7d88bb..f3878e2 100644 --- a/conf/tests/liftover_hg37.config +++ b/conf/tests/liftover_test.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_sv_hg37.csv' + input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_sv_liftover.csv" outdir = 'results' genome = 'GRCh37' @@ -28,14 +28,15 @@ params { analysis = 'germline' variant_type = "structural" method = 'truvari' - preprocess = "normalization,deduplication,filter_contigs" + preprocess = "filter_contigs" min_sv_size = 30 truth_id = "HG002" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" //liftover files - liftover = true + liftover = "test" chain = "http://ftp.ensembl.org/pub/assembly_mapping/homo_sapiens/GRCh38_to_GRCh37.chain.gz" rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch38_grch37.txt" } diff --git a/conf/tests/liftover_hg38.config b/conf/tests/liftover_truth.config similarity index 98% rename from conf/tests/liftover_hg38.config rename to conf/tests/liftover_truth.config index ae8a2ba..3ab7d01 100644 --- a/conf/tests/liftover_hg38.config +++ b/conf/tests/liftover_truth.config @@ -35,7 +35,7 @@ params { regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" //liftover files - liftover = true + liftover = "truth" chain = "https://raw.githubusercontent.com/broadinstitute/gatk/master/scripts/funcotator/data_sources/gnomAD/b37ToHg38.over.chain" rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" } diff --git a/nextflow.config b/nextflow.config index c655a87..f1e969b 100644 --- a/nextflow.config +++ b/nextflow.config @@ -48,7 +48,7 @@ params { dictionary = null rename_chr = null chain = null - liftover = false + liftover = "" // MultiQC options multiqc_config = null @@ -193,8 +193,8 @@ profiles { somatic_snv { includeConfig 'conf/tests/somatic_snv.config' } somatic_indel { includeConfig 'conf/tests/somatic_indel.config' } somatic_sv { includeConfig 'conf/tests/somatic_sv.config' } - liftover_hg37 { includeConfig 'conf/tests/liftover_hg37.config' } - liftover_hg38 { includeConfig 'conf/tests/liftover_hg38.config' } + liftover_test { includeConfig 'conf/tests/liftover_test.config' } + liftover_truth { includeConfig 'conf/tests/liftover_truth.config' } } diff --git a/nextflow_schema.json b/nextflow_schema.json index c9d2a76..517cbcd 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -31,12 +31,14 @@ }, "truth_id": { "type": "string", + "default": "", "description": "Truth id, sample name to define truth vcf", "fa_icon": "fas fa-folder-open", "errorMessage": "The sample name of the truth case. Examples: HG002, SEQC2, HG001, HG003, CHM13" }, "analysis": { "type": "string", + "default": "", "description": "The analysis type used by the input files", "enum": ["germline", "somatic"], "pattern": "(germline|somatic)", @@ -45,6 +47,7 @@ }, "variant_type": { "type": "string", + "default": "", "description": "Variant types to benchmark", "errorMessage": "Select a variant type to make the analysis: small,snv,indel,structural or copynumber. Select small when your vcf contains both snvs and indels", "enum": ["small", "snv", "indel", "structural", "copynumber"], @@ -53,6 +56,7 @@ }, "method": { "type": "string", + "default": "truvari,svanalyzer,happy,sompy,rtgtools,wittyer", "description": "The benchmarking methods to use. Should be a comma-separate list of one or more of the following options: truvari, svanalyzer, happy, sompy, rtgtools, wittyer", "errorMessage": "A wrong input has been detected. Should be a comma-separated list of one or more of the following options: truvari, svanalyzer, happy, sompy, rtgtools, wittyer", "pattern": "^((truvari|svanalyzer|happy|sompy|rtgtools|wittyer)?,?)*(? tuple([id: params.truth_id], file)}, + ch_bed.map{file -> tuple([id: params.truth_id], file)}, chain.map{_meta, file -> file} ) versions = versions.mix(UCSC_LIFTOVER.out.versions.first()) diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index fdf2c32..69e0ae0 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -2,13 +2,14 @@ // PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS // -include { VCF_REHEADER_SAMPLENAME } from '../local/vcf_reheader_samplename' -include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' -include { VCF_VARIANT_FILTERING } from '../local/vcf_variant_filtering' -include { SPLIT_SMALL_VARIANTS_TEST } from '../local/split_small_variants_test' -include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' -include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' +include { VCF_REHEADER_SAMPLENAME } from '../local/vcf_reheader_samplename' +include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' +include { VCF_VARIANT_FILTERING } from '../local/vcf_variant_filtering' +include { SPLIT_SMALL_VARIANTS_TEST } from '../local/split_small_variants_test' +include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' +include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' +include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' +include { LIFTOVER_VCFS } from '../local/liftover_vcfs' include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_CONTIGS } from '../../modules/nf-core/bcftools/view' @@ -17,14 +18,35 @@ workflow PREPARE_VCFS_TEST { test_ch // channel: [val(meta), vcf] fasta // reference channel [val(meta), ref.fa] fai // reference channel [val(meta), ref.fa.fai] + chain // reference channel [val(meta), chain.gz] + rename_chr // reference channel [val(meta), chrlist.txt] + dictionary // reference channel [val(meta), genome.dict] main: versions = Channel.empty() + test_ch.branch{ + def meta = it[0] + liftover: meta.liftover + other: true}.set{vcf} + + vcf_ch = Channel.empty() + + LIFTOVER_VCFS( + vcf.liftover, + Channel.empty(), + fasta, + chain, + rename_chr, + dictionary + ) + versions = versions.mix(LIFTOVER_VCFS.out.versions.first()) + vcf_ch = vcf_ch.mix(LIFTOVER_VCFS.out.vcf_ch,vcf.other) + // Add "query" to test sample VCF_REHEADER_SAMPLENAME( - test_ch, + vcf_ch, fai ) versions = versions.mix(VCF_REHEADER_SAMPLENAME.out.versions.first()) diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 1f6b372..1548741 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -7,7 +7,7 @@ include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' include { VCF_REHEADER_SAMPLENAME } from '../local/vcf_reheader_samplename' include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' -include { LIFTOVER_VCFS_TRUTH } from '../local/liftover_vcfs_truth' +include { LIFTOVER_VCFS } from '../local/liftover_vcfs' workflow PREPARE_VCFS_TRUTH { @@ -25,9 +25,9 @@ workflow PREPARE_VCFS_TRUTH { versions = Channel.empty() // if liftover option is set convert truth files - if (params.liftover){ + if (params.liftover.contains("truth")){ - LIFTOVER_VCFS_TRUTH( + LIFTOVER_VCFS( truth_ch, high_conf_ch, fasta, @@ -35,9 +35,9 @@ workflow PREPARE_VCFS_TRUTH { rename_chr, dictionary ) - versions = versions.mix(LIFTOVER_VCFS_TRUTH.out.versions.first()) - truth_ch = LIFTOVER_VCFS_TRUTH.out.vcf_ch - high_conf_ch = LIFTOVER_VCFS_TRUTH.out.bed_ch.map{ _meta, bed -> [bed]} + versions = versions.mix(LIFTOVER_VCFS.out.versions.first()) + truth_ch = LIFTOVER_VCFS.out.vcf_ch + high_conf_ch = LIFTOVER_VCFS.out.bed_ch.map{ _meta, bed -> [bed]} } // Reheader sample name for truth file - using meta.caller diff --git a/tests/liftover_test.nf.test b/tests/liftover_test.nf.test new file mode 100644 index 0000000..b049a2b --- /dev/null +++ b/tests/liftover_test.nf.test @@ -0,0 +1,40 @@ +nextflow_pipeline { + + name "Test pipeline for liftover structural germline variants, tested benchmarking methods are truvari" + script "../main.nf" + tag "pipeline" + tag "structural" + tag "germline" + tag "liftover" + tag "hg37" + config "../conf/tests/liftover_test.config" + + test("Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover 'test'") { + + when { + params { + outdir = "$outputDir" + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/liftover_test.nf.test.snap b/tests/liftover_test.nf.test.snap new file mode 100644 index 0000000..7dfaaf1 --- /dev/null +++ b/tests/liftover_test.nf.test.snap @@ -0,0 +1,208 @@ +{ + "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover 'test'": { + "content": [ + 67, + { + "BCFTOOLS_REHEADER": { + "bcftools": 1.18 + }, + "BCFTOOLS_STATS": { + "bcftools": 1.18 + }, + "BCFTOOLS_VIEW_CONTIGS": { + "bcftools": 1.18 + }, + "BGZIP_TABIX": { + "tabix": 1.12 + }, + "DATAVZRD": { + "datavzrd": "2.36.12" + }, + "MERGE_REPORTS": { + "python": "3.8.6" + }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, + "PLOTS": { + "r-base": "4.3.1" + }, + "SURVIVOR_MERGE": { + "survivor": "1.0.7" + }, + "SURVIVOR_STATS": { + "survivor": "1.0.7" + }, + "TABIX_BGZIP": { + "tabix": "1.19.1" + }, + "TABIX_BGZIPTABIX": { + "tabix": "1.19.1" + }, + "TABIX_TABIX": { + "tabix": "1.19.1" + }, + "TRUVARI_BENCH": { + "truvari": "4.1.0)" + }, + "VCF_TO_CSV": { + "python": "3.12.4" + }, + "Workflow": { + "nf-core/variantbenchmarking": "v1.0dev" + } + }, + [ + "pipeline_info", + "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "references", + "references/dictionary", + "references/dictionary/genome.dict", + "structural", + "structural/HG002", + "structural/HG002/liftover", + "structural/HG002/liftover/test2.renamechr.vcf.gz", + "structural/HG002/stats", + "structural/HG002/stats/bcftools", + "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", + "structural/HG002/stats/survivor", + "structural/HG002/stats/survivor/HG002_mqc.stats", + "structural/multiqc", + "structural/multiqc/multiqc_data", + "structural/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", + "structural/multiqc/multiqc_data/multiqc.log", + "structural/multiqc/multiqc_data/multiqc_bcftools_stats.txt", + "structural/multiqc/multiqc_data/multiqc_citations.txt", + 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"structural/summary/datavzrd/truvari/test/plots/plot_6.js", + "structural/summary/datavzrd/truvari/test/plots/plot_7.js", + "structural/summary/datavzrd/versions.yml", + "structural/summary/plots", + "structural/summary/plots/truvari", + "structural/summary/plots/truvari/metric_by_tool_truvari_mqc.png", + "structural/summary/plots/truvari/variants_by_tool_truvari_mqc.png", + "structural/summary/tables", + "structural/summary/tables/truvari", + "structural/summary/tables/truvari/truvari.summary.csv", + "structural/test1", + "structural/test1/benchmarks", + "structural/test1/benchmarks/truvari", + "structural/test1/benchmarks/truvari/test1.HG002.delly.fn.vcf.gz", + "structural/test1/benchmarks/truvari/test1.HG002.delly.fn.vcf.gz.tbi", + "structural/test1/benchmarks/truvari/test1.HG002.delly.fp.vcf.gz", + "structural/test1/benchmarks/truvari/test1.HG002.delly.fp.vcf.gz.tbi", + "structural/test1/benchmarks/truvari/test1.HG002.delly.summary.json", + "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-base.vcf.gz", + "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-base.vcf.gz.tbi", + "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz", + "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz.tbi", + "structural/test1/preprocess", + "structural/test1/preprocess/test1.filter.vcf", + "structural/test1/stats", + "structural/test1/stats/bcftools", + "structural/test1/stats/bcftools/test1.delly.bcftools_stats.txt", + "structural/test1/stats/survivor", + "structural/test1/stats/survivor/test1.delly_mqc.stats", + "structural/test2", + "structural/test2/benchmarks", + "structural/test2/benchmarks/truvari", + "structural/test2/benchmarks/truvari/test2.HG002.manta.fn.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.manta.fn.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.manta.fp.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.manta.fp.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.manta.summary.json", + "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-base.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-base.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz.tbi", + "structural/test2/preprocess", + "structural/test2/preprocess/test2.filter.vcf", + "structural/test2/stats", + "structural/test2/stats/bcftools", + "structural/test2/stats/bcftools/test2.manta.bcftools_stats.txt", + "structural/test2/stats/survivor", + "structural/test2/stats/survivor/test2.manta_mqc.stats" + ], + [ + "HG002.bcftools_stats.txt:md5,7c007a87b5730787e570712e784d3cc3", + "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", + "test1.delly.bcftools_stats.txt:md5,d32a9bea755d2065e19e88b9159e2502", + "test1.delly_mqc.stats:md5,e140ad55975c767578b0dd6aff58ba29", + "test2.manta.bcftools_stats.txt:md5,99f8a0b12efa30c6253b44ebfeb9b1d6", + "test2.manta_mqc.stats:md5,1d74b41c6b970992e3a39682b0a68e23" + ] + ], + "meta": { + "nf-test": "0.9.0", + "nextflow": "24.10.2" + }, + "timestamp": "2024-12-03T15:52:10.533611573" + } +} \ No newline at end of file diff --git a/tests/liftover_hg38.nf.test b/tests/liftover_truth.nf.test similarity index 87% rename from tests/liftover_hg38.nf.test rename to tests/liftover_truth.nf.test index 00d46af..f3e94ce 100644 --- a/tests/liftover_hg38.nf.test +++ b/tests/liftover_truth.nf.test @@ -1,15 +1,15 @@ nextflow_pipeline { - name "Test pipeline for small germline variants, tested benchmarking methods are happy and rtgtools" + name "Test pipeline for liftover small germline variants, tested benchmarking methods are happy and rtgtools" script "../main.nf" tag "pipeline" tag "small" tag "germline" tag "liftover" tag "hg38" - config "../conf/tests/liftover_hg38.config" + config "../conf/tests/liftover_truth.config" - test("Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover") { + test("Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover 'truth'") { when { params { diff --git a/tests/liftover_hg38.nf.test.snap b/tests/liftover_truth.nf.test.snap similarity index 59% rename from tests/liftover_hg38.nf.test.snap rename to tests/liftover_truth.nf.test.snap index 55af699..fc319d7 100644 --- a/tests/liftover_hg38.nf.test.snap +++ b/tests/liftover_truth.nf.test.snap @@ -1,7 +1,7 @@ { - "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover": { + "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover 'truth'": { "content": [ - 85, + 86, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -33,6 +33,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -351,264 +354,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-11-29T09:23:31.648065377" - }, - "-stub": { - "content": [ - 85, - { - "BCFTOOLS_DEDUP": { - "bcftools": 1.18 - }, - "BCFTOOLS_MERGE": { - "bcftools": 1.2 - }, - "BCFTOOLS_NORM": { - "bcftools": 1.18 - }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.18 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "HAPPY_HAPPY": { - "hap.py": "0.3.14" - }, - "MERGE_REPORTS": { - "python": "3.8.6" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "REFORMAT_HEADER": { - "tabix": 1.12 - }, - "RTGTOOLS_FORMAT": { - "rtg-tools": "3.12.1" - }, - "RTGTOOLS_VCFEVAL": { - "rtg-tools": "3.12.1" - }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, - "TABIX_TABIX": { - "tabix": "1.19.1" - }, - "UCSC_LIFTOVER": { - "ucsc": 377 - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, - [ - "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", - "references", - "references/dictionary", - "references/dictionary/genome.dict", - "references/rtgtools", - "references/rtgtools/genome.sdf", - "small", - "small/HG002", - "small/HG002/liftover", - "small/HG002/liftover/HG002.renamechr.vcf.gz", - "small/HG002/liftover/HG002.sort.merged.bed", - "small/HG002/preprocess", - "small/HG002/preprocess/HG002.renamechr.rh.norm.dedup.sort.vcf.gz", - "small/HG002/stats", - "small/HG002/stats/bcftools", - "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", - "small/multiqc", - "small/multiqc/multiqc_data", - "small/multiqc/multiqc_plots", - "small/multiqc/multiqc_report.html", - "small/summary", - "small/summary/comparisons", - "small/summary/comparisons/small", - "small/summary/comparisons/small/rtgtools.FN.csv", - "small/summary/comparisons/small/rtgtools.FP.csv", - "small/summary/comparisons/small/rtgtools.TP_base.csv", - "small/summary/comparisons/small/rtgtools.TP_comp.csv", - "small/summary/datavzrd", - "small/summary/datavzrd/happy", - "small/summary/datavzrd/happy/index.html", - "small/summary/datavzrd/happy/network", - "small/summary/datavzrd/happy/network/config.js", - "small/summary/datavzrd/happy/network/data", - "small/summary/datavzrd/happy/network/data/data_1.js", - "small/summary/datavzrd/happy/network/functions.js", - "small/summary/datavzrd/happy/network/heatmap.js", - "small/summary/datavzrd/happy/network/index_1.html", - "small/summary/datavzrd/happy/network/plots", - "small/summary/datavzrd/happy/network/plots/plot_0.js", - "small/summary/datavzrd/happy/static", - "small/summary/datavzrd/happy/static/bootstrap-select.min.css", - "small/summary/datavzrd/happy/static/bootstrap-table-fixed-columns.min.css", - "small/summary/datavzrd/happy/static/bootstrap-table.min.css", - "small/summary/datavzrd/happy/static/bootstrap.min.css", - "small/summary/datavzrd/happy/static/bundle.js", - "small/summary/datavzrd/happy/static/datavzrd.css", - "small/summary/datavzrd/rtgtools", - "small/summary/datavzrd/rtgtools/index.html", - "small/summary/datavzrd/rtgtools/network", - "small/summary/datavzrd/rtgtools/network/config.js", - "small/summary/datavzrd/rtgtools/network/data", - "small/summary/datavzrd/rtgtools/network/data/data_1.js", - "small/summary/datavzrd/rtgtools/network/functions.js", - "small/summary/datavzrd/rtgtools/network/heatmap.js", - "small/summary/datavzrd/rtgtools/network/index_1.html", - "small/summary/datavzrd/rtgtools/network/plots", - "small/summary/datavzrd/rtgtools/network/plots/plot_0.js", - "small/summary/datavzrd/rtgtools/static", - "small/summary/datavzrd/rtgtools/static/bootstrap-select.min.css", - "small/summary/datavzrd/rtgtools/static/bootstrap-table-fixed-columns.min.css", - "small/summary/datavzrd/rtgtools/static/bootstrap-table.min.css", - "small/summary/datavzrd/rtgtools/static/bootstrap.min.css", - "small/summary/datavzrd/rtgtools/static/bundle.js", - "small/summary/datavzrd/rtgtools/static/datavzrd.css", - "small/summary/datavzrd/versions.yml", - "small/summary/plots", - "small/summary/plots/happy", - "small/summary/plots/happy/metric_by_tool_happy.png", - "small/summary/plots/happy/variants_by_tool_happy.png", - "small/summary/plots/rtgtools", - "small/summary/plots/rtgtools/metric_by_tool_rtgtools.png", - "small/summary/plots/rtgtools/variants_by_tool_rtgtools.png", - "small/summary/tables", - "small/summary/tables/happy", - "small/summary/tables/happy/happy.regions.csv", - "small/summary/tables/happy/happy.summary.csv", - "small/summary/tables/rtgtools", - "small/summary/tables/rtgtools/rtgtools.regions.csv", - "small/summary/tables/rtgtools/rtgtools.summary.csv", - "small/test1", - "small/test1/benchmarks", - "small/test1/benchmarks/happy", - "small/test1/benchmarks/happy/test1.HG002.strelka.extended.csv", - "small/test1/benchmarks/happy/test1.HG002.strelka.metrics.json.gz", - "small/test1/benchmarks/happy/test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz", - "small/test1/benchmarks/happy/test1.HG002.strelka.roc.Locations.INDEL.csv.gz", - "small/test1/benchmarks/happy/test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz", - "small/test1/benchmarks/happy/test1.HG002.strelka.roc.Locations.SNP.csv.gz", - "small/test1/benchmarks/happy/test1.HG002.strelka.roc.all.csv.gz", - "small/test1/benchmarks/happy/test1.HG002.strelka.runinfo.json", - "small/test1/benchmarks/happy/test1.HG002.strelka.summary.csv", - "small/test1/benchmarks/happy/test1.HG002.strelka.vcf.gz", - "small/test1/benchmarks/happy/test1.HG002.strelka.vcf.gz.tbi", - "small/test1/benchmarks/rtgtools", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.fn.vcf.gz", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.fn.vcf.gz.tbi", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.fp.vcf.gz", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.fp.vcf.gz.tbi", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.non_snp_roc.tsv.gz", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.phasing.txt", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.snp_roc.tsv.gz", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.summary.txt", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp-baseline.vcf.gz", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp-baseline.vcf.gz.tbi", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp.vcf.gz", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp.vcf.gz.tbi", - "small/test1/benchmarks/rtgtools/test1.HG002.strelka.weighted_roc.tsv.gz", - "small/test1/preprocess", - "small/test1/preprocess/test1.dedup.sort.vcf.gz", - "small/test1/stats", - "small/test1/stats/bcftools", - "small/test1/stats/bcftools/test1.strelka.bcftools_stats.txt", - "small/test2", - "small/test2/benchmarks", - "small/test2/benchmarks/happy", - "small/test2/benchmarks/happy/test2.HG002.bcftools.extended.csv", - "small/test2/benchmarks/happy/test2.HG002.bcftools.metrics.json.gz", - "small/test2/benchmarks/happy/test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz", - "small/test2/benchmarks/happy/test2.HG002.bcftools.roc.Locations.INDEL.csv.gz", - "small/test2/benchmarks/happy/test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz", - "small/test2/benchmarks/happy/test2.HG002.bcftools.roc.Locations.SNP.csv.gz", - "small/test2/benchmarks/happy/test2.HG002.bcftools.roc.all.csv.gz", - "small/test2/benchmarks/happy/test2.HG002.bcftools.runinfo.json", - "small/test2/benchmarks/happy/test2.HG002.bcftools.summary.csv", - "small/test2/benchmarks/happy/test2.HG002.bcftools.vcf.gz", - "small/test2/benchmarks/happy/test2.HG002.bcftools.vcf.gz.tbi", - "small/test2/benchmarks/rtgtools", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.fn.vcf.gz", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.fn.vcf.gz.tbi", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.fp.vcf.gz", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.fp.vcf.gz.tbi", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.non_snp_roc.tsv.gz", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.phasing.txt", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.snp_roc.tsv.gz", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.summary.txt", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp-baseline.vcf.gz", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp-baseline.vcf.gz.tbi", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp.vcf.gz", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp.vcf.gz.tbi", - "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.weighted_roc.tsv.gz", - "small/test2/preprocess", - "small/test2/preprocess/test2.dedup.sort.vcf.gz", - "small/test2/stats", - "small/test2/stats/bcftools", - "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" - ], - [ - "genome.sdf:md5,d41d8cd98f00b204e9800998ecf8427e", - "HG002.sort.merged.bed:md5,d41d8cd98f00b204e9800998ecf8427e", - "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "multiqc_plots:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "config.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "data_1.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "functions.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "heatmap.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "index_1.html:md5,d41d8cd98f00b204e9800998ecf8427e", - "plot_0.js:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.HG002.strelka.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test1.HG002.strelka.roc.all.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test1.HG002.strelka.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.HG002.strelka.phasing.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.HG002.strelka.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test1.strelka.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.bcftools.extended.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test2.HG002.bcftools.roc.Locations.SNP.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test2.HG002.bcftools.roc.all.csv.gz:md5,68b329da9893e34099c7d8ad5cb9c940", - "test2.HG002.bcftools.summary.csv:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.bcftools.phasing.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.bcftools.summary.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.bcftools.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e" - ] - ], - "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.0" - }, - "timestamp": "2024-11-12T15:39:05.089607077" + "timestamp": "2024-12-03T15:58:16.618582714" } } \ No newline at end of file diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 0cd825e..2c78a31 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -67,20 +67,24 @@ workflow VARIANTBENCHMARKING { : Channel.empty() // read chain file, liftover genome and rename chr files if liftover is true - chain = Channel.empty() - rename_chr = Channel.empty() - dictionary = Channel.empty() - if (params.liftover){ - chain = params.chain ? Channel.fromPath(params.chain, checkIfExists: true).map{ bed -> tuple([id: bed.getSimpleName()], bed) }.collect() - : Channel.empty() - rename_chr = params.rename_chr ? Channel.fromPath(params.rename_chr, checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() - : Channel.empty() + if (params.chain && params.rename_chr){ + chain = Channel.fromPath(params.chain, checkIfExists: true).map{ bed -> tuple([id: bed.getSimpleName()], bed) }.collect() + rename_chr = Channel.fromPath(params.rename_chr, checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() + }else{ + log.error "Please specify params.chain and params.rename_chr to process liftover of the files" + exit 1 + } - dictionary = params.dictionary ? Channel.fromPath(params.dictionary, checkIfExists: true).map{ dict -> tuple([id: dict.getSimpleName()], dict) }.collect() - : Channel.empty() + // if dictinoary file is missing PICARD_CREATESEQUENCEDICTIONARY will create one + dictionary = params.dictionary ? Channel.fromPath(params.dictionary, checkIfExists: true).map{ dict -> tuple([id: dict.getSimpleName()], dict) }.collect() : Channel.empty() + }else{ + chain = Channel.empty() + rename_chr = Channel.empty() + dictionary = Channel.empty() } + // PREPROCESSES // subsample multisample vcf if necessary @@ -115,7 +119,10 @@ workflow VARIANTBENCHMARKING { PREPARE_VCFS_TEST( vcf_ch, fasta, - fai + fai, + chain, + rename_chr, + dictionary ) ch_versions = ch_versions.mix(PREPARE_VCFS_TEST.out.versions) From c687f926c7e05408c0cf78bb59e595e747f32956 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 3 Dec 2024 16:28:15 +0000 Subject: [PATCH 219/292] edit default --- nextflow_schema.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 517cbcd..ed1d723 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -38,7 +38,7 @@ }, "analysis": { "type": "string", - "default": "", + "default": "germline", "description": "The analysis type used by the input files", "enum": ["germline", "somatic"], "pattern": "(germline|somatic)", @@ -47,7 +47,7 @@ }, "variant_type": { "type": "string", - "default": "", + "default": "small", "description": "Variant types to benchmark", "errorMessage": "Select a variant type to make the analysis: small,snv,indel,structural or copynumber. Select small when your vcf contains both snvs and indels", "enum": ["small", "snv", "indel", "structural", "copynumber"], From 4c82c9ace2c9ebf1722adbc943d47b616f0327ec Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 3 Dec 2024 16:39:42 +0000 Subject: [PATCH 220/292] error handling with truth --- conf/tests/liftover_test.config | 2 +- nextflow_schema.json | 3 --- workflows/variantbenchmarking.nf | 10 ++++++++-- 3 files changed, 9 insertions(+), 6 deletions(-) diff --git a/conf/tests/liftover_test.config b/conf/tests/liftover_test.config index f3878e2..937ea4f 100644 --- a/conf/tests/liftover_test.config +++ b/conf/tests/liftover_test.config @@ -32,7 +32,7 @@ params { min_sv_size = 30 truth_id = "HG002" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + //truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" //liftover files diff --git a/nextflow_schema.json b/nextflow_schema.json index ed1d723..446b0f0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -31,14 +31,12 @@ }, "truth_id": { "type": "string", - "default": "", "description": "Truth id, sample name to define truth vcf", "fa_icon": "fas fa-folder-open", "errorMessage": "The sample name of the truth case. Examples: HG002, SEQC2, HG001, HG003, CHM13" }, "analysis": { "type": "string", - "default": "germline", "description": "The analysis type used by the input files", "enum": ["germline", "somatic"], "pattern": "(germline|somatic)", @@ -47,7 +45,6 @@ }, "variant_type": { "type": "string", - "default": "small", "description": "Variant types to benchmark", "errorMessage": "Select a variant type to make the analysis: small,snv,indel,structural or copynumber. Select small when your vcf contains both snvs and indels", "enum": ["small", "snv", "indel", "structural", "copynumber"], diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 2c78a31..aa23d3a 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -56,8 +56,14 @@ workflow VARIANTBENCHMARKING { //// check Truth Files //// - truth_ch = Channel.fromPath(params.truth_vcf, checkIfExists: true) - .map{ vcf -> tuple([id: params.truth_id, vartype:params.variant_type], vcf) }.collect() + if (params.truth_id && params.truth_ch){ + truth_ch = Channel.fromPath(params.truth_vcf, checkIfExists: true) + .map{ vcf -> tuple([id: params.truth_id, vartype:params.variant_type], vcf) }.collect() + }else{ + log.error "Please specify params.truth_id and params.truth_vcf to perform benchmarking analysis" + exit 1 + } + regions_bed_ch = params.regions_bed ? Channel.fromPath(params.regions_bed, checkIfExists: true).collect() : Channel.empty() From 2f14200c08fa60fc845f91591f9ffafd40b268f8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 3 Dec 2024 16:40:51 +0000 Subject: [PATCH 221/292] chande default for methods --- nextflow_schema.json | 1 - 1 file changed, 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 446b0f0..fc4b2f4 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -53,7 +53,6 @@ }, "method": { "type": "string", - "default": "truvari,svanalyzer,happy,sompy,rtgtools,wittyer", "description": "The benchmarking methods to use. Should be a comma-separate list of one or more of the following options: truvari, svanalyzer, happy, sompy, rtgtools, wittyer", "errorMessage": "A wrong input has been detected. Should be a comma-separated list of one or more of the following options: truvari, svanalyzer, happy, sompy, rtgtools, wittyer", "pattern": "^((truvari|svanalyzer|happy|sompy|rtgtools|wittyer)?,?)*(? Date: Tue, 3 Dec 2024 16:48:43 +0000 Subject: [PATCH 222/292] wrong decleration of params.truth_vcf --- workflows/variantbenchmarking.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index aa23d3a..455dbbd 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -56,7 +56,7 @@ workflow VARIANTBENCHMARKING { //// check Truth Files //// - if (params.truth_id && params.truth_ch){ + if (params.truth_id && params.truth_vcf){ truth_ch = Channel.fromPath(params.truth_vcf, checkIfExists: true) .map{ vcf -> tuple([id: params.truth_id, vartype:params.variant_type], vcf) }.collect() }else{ From 57fd7c7423a11ddb066982b73b0f5d1ea0f3d621 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 3 Dec 2024 17:15:28 +0000 Subject: [PATCH 223/292] fix --- conf/tests/liftover_test.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/tests/liftover_test.config b/conf/tests/liftover_test.config index 937ea4f..f3878e2 100644 --- a/conf/tests/liftover_test.config +++ b/conf/tests/liftover_test.config @@ -32,7 +32,7 @@ params { min_sv_size = 30 truth_id = "HG002" - //truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" //liftover files From 70214d23c361f05a6d284820538b5daac1bdd193 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 4 Dec 2024 10:24:48 +0000 Subject: [PATCH 224/292] renew snapshots --- tests/germline_small.nf.test.snap | 18 ++++++++++++++---- tests/germline_sv.nf.test.snap | 20 ++++++++++++++++---- tests/somatic_indel.nf.test.snap | 14 ++++++++++---- tests/somatic_snv.nf.test.snap | 12 +++++++++--- tests/somatic_sv.nf.test.snap | 10 ++++++++-- 5 files changed, 57 insertions(+), 17 deletions(-) diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index a99138b..e1a3bb6 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -1,7 +1,7 @@ { "-stub": { "content": [ - 76, + 77, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -33,6 +33,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -62,6 +65,8 @@ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "references", + "references/dictionary", + "references/dictionary/genome.dict", "references/rtgtools", "references/rtgtools/genome.sdf", "small", @@ -231,11 +236,11 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-11-28T16:47:47.024615694" + "timestamp": "2024-12-04T10:12:17.779754389" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ - 76, + 77, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -267,6 +272,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -296,6 +304,8 @@ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "references", + "references/dictionary", + "references/dictionary/genome.dict", "references/rtgtools", "references/rtgtools/genome.sdf", "references/rtgtools/genome.sdf/done", @@ -575,6 +585,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-11-28T16:45:26.990755841" + "timestamp": "2024-12-04T10:10:09.338722844" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index b566380..5181ca9 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'": { "content": [ - 144, + 145, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -30,6 +30,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -79,6 +82,9 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "references", + "references/dictionary", + "references/dictionary/genome.dict", "structural", "structural/HG002", "structural/HG002/preprocess", @@ -357,11 +363,11 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-11-28T16:51:57.386421371" + "timestamp": "2024-12-04T10:22:09.959009433" }, "-stub": { "content": [ - 144, + 145, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -390,6 +396,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -439,6 +448,9 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "references", + "references/dictionary", + "references/dictionary/genome.dict", "structural", "structural/HG002", "structural/HG002/preprocess", @@ -656,6 +668,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-11-28T16:54:34.483191183" + "timestamp": "2024-12-04T10:24:27.339868009" } } \ No newline at end of file diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 6107271..2e1d10b 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'somatic' --variant_type 'indel' --method 'sompy'": { "content": [ - 27, + 28, { "BCFTOOLS_REHEADER": { "bcftools": 1.18 @@ -30,6 +30,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -165,7 +168,10 @@ "indel/test2/stats/bcftools", "indel/test2/stats/bcftools/test2.strelka.bcftools_stats.txt", "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml" + "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "references", + "references/dictionary", + "references/dictionary/genome.dict" ], [ "SEQC2.bcftools_stats.txt:md5,e530daf4f6a4923f1cd85c51893d5747", @@ -175,8 +181,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-22T12:53:42.07320983" + "timestamp": "2024-12-04T09:59:41.934881268" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 4c4cccb..20188bb 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -1,7 +1,7 @@ { "-stub": { "content": [ - 38, + 39, { "BCFTOOLS_REHEADER": { "bcftools": 1.18 @@ -30,6 +30,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -46,6 +49,9 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "references", + "references/dictionary", + "references/dictionary/genome.dict", "snv", "snv/SEQC2", "snv/SEQC2/stats", @@ -125,8 +131,8 @@ ], "meta": { "nf-test": "0.9.0", - "nextflow": "24.10.1" + "nextflow": "24.10.2" }, - "timestamp": "2024-11-22T12:57:55.883023408" + "timestamp": "2024-12-04T09:53:59.901867572" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index fabf609..539def8 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'somatic' --variant_type 'structural' --method 'truvari,svbenchmark'": { "content": [ - 69, + 70, { "BCFTOOLS_REHEADER": { "bcftools": 1.18 @@ -30,6 +30,9 @@ "MERGE_REPORTS": { "python": "3.8.6" }, + "PICARD_CREATESEQUENCEDICTIONARY": { + "picard": "3.2.0-1-g3948afb6b" + }, "PLOTS": { "r-base": "4.3.1" }, @@ -61,6 +64,9 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "references", + "references/dictionary", + "references/dictionary/genome.dict", "structural", "structural/SEQC2", "structural/SEQC2/stats", @@ -206,6 +212,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-11-28T16:58:12.671419675" + "timestamp": "2024-12-04T09:56:29.305188123" } } \ No newline at end of file From d7ed58ac4786b11e92e8d127e94d824878337271 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 5 Dec 2024 09:17:46 +0000 Subject: [PATCH 225/292] update link to truth.md --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index e56db2c..8f4d274 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -41,7 +41,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p ## Truth samples -Please find the detailed information about truth samples [here](/workspace/variantbenchmarking/docs/truth.md). +Please find the detailed information about truth samples [here](../docs/truth.md). ## Lifting over truth sets From f7c496cd7def2e1b7f6b2d0a760f5111c83074e2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 5 Dec 2024 12:35:46 +0000 Subject: [PATCH 226/292] update usage.md --- docs/usage.md | 22 ++++++++++++++++++---- 1 file changed, 18 insertions(+), 4 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 8f4d274..7471a45 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -45,10 +45,24 @@ Please find the detailed information about truth samples [here](../docs/truth.md ## Lifting over truth sets -This workflow comes with a liftover option for truth sets. In order to activate liftover use `--liftover true`. +This workflow comes with a liftover option for truth sets. In order to activate liftover use `--liftover "truth"`. - `--chain`: This workflow uses picard tools for lifting over and a chain file has to be provided specific to the input truth vcf. Some examples can be found [here](https://genome.ucsc.edu/goldenPath/help/chain.html) - `--rename_chr`: Renaming chromosomes is required after liftover process. Some examples can be found under `assets/rename_contigs` directory. +- `--dictionary`: .dict file is required to run liftover process. If dictionary file is not provided, picard createsequencedictionary will create and use the file. + +## Lifting over test sets + +Lifting over test samples is also possible through this pipeline, if you want to liftover at least one of the samples first use `--liftover "test"` and add liftover option to samplesheet: + +```csv title="samplesheet.csv" +id,test_vcf,caller,liftover +test1,test1.vcf.gz,delly,true +test2,test2.vcf,gatk,false +test3,test3.vcf.gz,cnvkit,true +``` + +Please note that you should still provide chain and reame_chr files, and lifting over truth and test samples simultaneously is not possible. ## Standardization and normalization parameters @@ -234,10 +248,10 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - `test_full` - A profile with a complete configuration for full size of sample testing - Includes links to test data so needs no other parameters -- `liftover_hg37` - - A profile with a complete configuration for using liftover of HG002 hg38 truth set to hg37 +- `liftover_test` + - A profile with a complete configuration for using liftover of HG002 hg38 test set to hg37 - Includes links to test data so needs no other parameters -- `liftover_hg38` +- `liftover_truth` - A profile with a complete configuration for using liftover of HG002 hg37 truth set to hg38 - Includes links to test data so needs no other parameters - `germline_small` From ca2236a0d5b15f19c530afca030b596800735ef9 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 5 Dec 2024 14:21:55 +0000 Subject: [PATCH 227/292] add bcf to input --- README.md | 4 ++-- assets/schema_input.json | 4 ++-- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 9478981..39daff6 100644 --- a/README.md +++ b/README.md @@ -50,9 +50,9 @@ Applying filtering on the process of benchmarking itself might makes it impossib 10. Include or exclude SNVs and INDELs ([bcftools filter](https://samtools.github.io/bcftools/bcftools.html#filter)) 11. Size and quality filtering for SVs ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) -### Liftover of truth sets: +### Liftover of vcfs: -This sub-workflow provides option to convert genome coordinates of truth VCF and high confidence BED file to a new assembly. Golden standard truth files are build upon specific reference genomes which makes the necessity of lifting over depending on the test VCF in query. +This sub-workflow provides option to convert genome coordinates of truth VCF and high confidence BED file to a new assembly. Golden standard truth files are build upon specific reference genomes which makes the necessity of lifting over depending on the test VCF in query. Lifting over one or more test vcfs is also possible. 12. Create sequence dictionary for the reference ([picard CreateSequenceDictionary](https://gatk.broadinstitute.org/hc/en-us/articles/360037068312-CreateSequenceDictionary-Picard)). This file can be saved and reused. 13. Lifting over truth variants ([picard LiftoverVcf](https://gatk.broadinstitute.org/hc/en-us/articles/360037060932-LiftoverVcf-Picard)) diff --git a/assets/schema_input.json b/assets/schema_input.json index c6a6cf1..0839b98 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,9 +10,9 @@ "test_vcf": { "type": "string", "exists": true, - "pattern": "\\S+\\.vcf(\\.gz)?$", + "pattern": "\\S+\\.(vcf|bcf)(\\.gz)?$", "format": "file-path", - "errorMessage": "Test VCF must be provided, cannot contain spaces and must have extension '.vcf.gz' or '.vcf'", + "errorMessage": "Test VCF must be provided, cannot contain spaces and must have extension '.vcf.gz', '.vcf', '.bcf.gz' or 'bcf'", "description": "The VCF file to use as benchmarking test input" }, "id": { From 4a999663b7852f6cdc608bf73539aae5cd00c036 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 6 Dec 2024 16:01:09 +0000 Subject: [PATCH 228/292] add fix_vcf_prefix script --- assets/schema_input.json | 6 ++ bin/fix_vcf_prefix.py | 88 +++++++++++++++++++ .../custom/fix_vcf_prefix/environment.yml | 8 ++ modules/local/custom/fix_vcf_prefix/main.nf | 51 +++++++++++ subworkflows/local/prepare_vcfs_test.nf | 40 +++++++-- workflows/variantbenchmarking.nf | 27 ++++-- 6 files changed, 206 insertions(+), 14 deletions(-) create mode 100755 bin/fix_vcf_prefix.py create mode 100644 modules/local/custom/fix_vcf_prefix/environment.yml create mode 100644 modules/local/custom/fix_vcf_prefix/main.nf diff --git a/assets/schema_input.json b/assets/schema_input.json index 0839b98..f014e26 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -160,6 +160,12 @@ "description": "Liftover option for test vcfs, to activate add params.liftover='test' ", "meta": ["liftover"], "default": false + }, + "fix_prefix": { + "type": "boolean", + "description": "Fixing prefix is sometimes necessary for input files, activate it if your input is not adhering to GRCh38 with chr and GRCh37 without chr standards ", + "meta": ["fix_prefix"], + "default": false } }, "required": ["test_vcf", "caller", "id"] diff --git a/bin/fix_vcf_prefix.py b/bin/fix_vcf_prefix.py new file mode 100755 index 0000000..adc0bf2 --- /dev/null +++ b/bin/fix_vcf_prefix.py @@ -0,0 +1,88 @@ +#!/usr/bin/env python + +# Copyright 2024 - GHGA +# Author: Kuebra Narci +''' +Generates a CSV file from a VCF +Expected usage: + $ python fix_vcf_prefix.py +Use --help for more information. +''' +import os +import subprocess +import argparse +import shutil + + +def determine_genome_version(vcf_file): + """ + Determine the genome version by inspecting chromosome naming in the VCF file. + """ + with subprocess.Popen( + f"bcftools view -h {vcf_file} | grep -m 1 '^##contig= versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + echo '' | gzip > ${prefix}.vcf.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bcftools: \$( bcftools --version |& sed '1!d; s/^.*bcftools //' ) + END_VERSIONS + """ +} diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 69e0ae0..9ba656e 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -11,6 +11,7 @@ include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgzip include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' include { LIFTOVER_VCFS } from '../local/liftover_vcfs' include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_CONTIGS } from '../../modules/nf-core/bcftools/view' +include { FIX_VCF_PREFIX } from '../../modules/local/custom/fix_vcf_prefix' workflow PREPARE_VCFS_TEST { @@ -33,16 +34,37 @@ workflow PREPARE_VCFS_TEST { vcf_ch = Channel.empty() - LIFTOVER_VCFS( - vcf.liftover, - Channel.empty(), - fasta, - chain, - rename_chr, - dictionary + if (params.liftover.contains("test")){ + LIFTOVER_VCFS( + vcf.liftover, + Channel.empty(), + fasta, + chain, + rename_chr, + dictionary + ) + versions = versions.mix(LIFTOVER_VCFS.out.versions.first()) + vcf_ch = vcf_ch.mix(LIFTOVER_VCFS.out.vcf_ch,vcf.other) + } + vcf_ch = vcf_ch.mix(vcf.other) + + // if prefix of chromosomes needs to be fixed + vcf_ch.branch{ + def meta = it[0] + prefix: meta.fix_prefix + other: true}.set{fix} + + vcf_ch = Channel.empty() + + fix.prefix.view() + + FIX_VCF_PREFIX( + fix.prefix, + rename_chr ) - versions = versions.mix(LIFTOVER_VCFS.out.versions.first()) - vcf_ch = vcf_ch.mix(LIFTOVER_VCFS.out.vcf_ch,vcf.other) + versions = versions.mix(FIX_VCF_PREFIX.out.versions.first()) + vcf_ch = vcf_ch.mix(FIX_VCF_PREFIX.out.vcf,fix.other) + // Add "query" to test sample VCF_REHEADER_SAMPLENAME( diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 455dbbd..0d6ee56 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -72,22 +72,39 @@ workflow VARIANTBENCHMARKING { sdf = params.sdf ? Channel.fromPath(params.sdf, checkIfExists: true).map{ sdf -> tuple([id: sdf.getSimpleName()], sdf) }.collect() : Channel.empty() + if (params.rename_chr){ + rename_chr = Channel.fromPath(params.rename_chr, checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() + if (!params.genome){ + log.error "Please specify params.genome to fix chromosome prefix" + exit 1 + } + + }else{ + if (params.genome == "GRCh38"){ + rename_chr = Channel.fromPath("assets/rename_contigs/grch37_grch38.txt", checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() + } + else if(params.genome == "GRCh37") + { + rename_chr = Channel.fromPath("assets/rename_contigs/grch38_grch37.txt", checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() + } + else{ + rename_chr = Channel.empty() + } + } + // read chain file, liftover genome and rename chr files if liftover is true if (params.liftover){ - if (params.chain && params.rename_chr){ + if (params.chain){ chain = Channel.fromPath(params.chain, checkIfExists: true).map{ bed -> tuple([id: bed.getSimpleName()], bed) }.collect() - rename_chr = Channel.fromPath(params.rename_chr, checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() }else{ - log.error "Please specify params.chain and params.rename_chr to process liftover of the files" + log.error "Please specify params.chain to process liftover of the files" exit 1 } - // if dictinoary file is missing PICARD_CREATESEQUENCEDICTIONARY will create one dictionary = params.dictionary ? Channel.fromPath(params.dictionary, checkIfExists: true).map{ dict -> tuple([id: dict.getSimpleName()], dict) }.collect() : Channel.empty() }else{ chain = Channel.empty() - rename_chr = Channel.empty() dictionary = Channel.empty() } From da8585f89663a392656999b2b1f97b7a568836bb Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 10 Dec 2024 11:28:18 +0000 Subject: [PATCH 229/292] renew snapshots --- conf/tests/germline_small.config | 2 ++ conf/tests/germline_sv.config | 1 + conf/tests/somatic_indel.config | 2 ++ conf/tests/somatic_snv.config | 2 ++ conf/tests/somatic_sv.config | 2 ++ subworkflows/local/prepare_vcfs_test.nf | 2 +- tests/germline_small.nf.test.snap | 18 ++++-------------- tests/germline_sv.nf.test.snap | 20 ++++---------------- tests/liftover_truth.nf.test.snap | 7 ++----- tests/somatic_indel.nf.test.snap | 12 +++--------- tests/somatic_sv.nf.test.snap | 10 ++-------- 11 files changed, 25 insertions(+), 53 deletions(-) diff --git a/conf/tests/germline_small.config b/conf/tests/germline_small.config index 8dcd613..514688c 100644 --- a/conf/tests/germline_small.config +++ b/conf/tests/germline_small.config @@ -40,4 +40,6 @@ params { truth_id = "HG002" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + } diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config index ddd89b4..dc724f3 100644 --- a/conf/tests/germline_sv.config +++ b/conf/tests/germline_sv.config @@ -41,4 +41,5 @@ params { truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" } diff --git a/conf/tests/somatic_indel.config b/conf/tests/somatic_indel.config index 463b096..d51111c 100644 --- a/conf/tests/somatic_indel.config +++ b/conf/tests/somatic_indel.config @@ -33,5 +33,7 @@ params { truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sINDEL_truth_set_v1.0.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + } diff --git a/conf/tests/somatic_snv.config b/conf/tests/somatic_snv.config index 6b80903..ec4c8c6 100644 --- a/conf/tests/somatic_snv.config +++ b/conf/tests/somatic_snv.config @@ -33,4 +33,6 @@ params { truth_id = "SEQC2" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sSNV_truth_set_v1.0.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + } diff --git a/conf/tests/somatic_sv.config b/conf/tests/somatic_sv.config index 02eeec2..7fdfe7e 100644 --- a/conf/tests/somatic_sv.config +++ b/conf/tests/somatic_sv.config @@ -32,4 +32,6 @@ params { truth_id = "SEQC2" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/13059_2022_2816_MOESM4_ESM.vcf.gz" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + } diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 9ba656e..2fadf16 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -44,7 +44,7 @@ workflow PREPARE_VCFS_TEST { dictionary ) versions = versions.mix(LIFTOVER_VCFS.out.versions.first()) - vcf_ch = vcf_ch.mix(LIFTOVER_VCFS.out.vcf_ch,vcf.other) + vcf_ch = vcf_ch.mix(LIFTOVER_VCFS.out.vcf_ch) } vcf_ch = vcf_ch.mix(vcf.other) diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index e1a3bb6..554f702 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -1,7 +1,7 @@ { "-stub": { "content": [ - 77, + 76, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -33,9 +33,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -65,8 +62,6 @@ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "references", - "references/dictionary", - "references/dictionary/genome.dict", "references/rtgtools", "references/rtgtools/genome.sdf", "small", @@ -236,11 +231,11 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-04T10:12:17.779754389" + "timestamp": "2024-12-10T10:55:37.880792825" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ - 77, + 76, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -272,9 +267,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -304,8 +296,6 @@ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "references", - "references/dictionary", - "references/dictionary/genome.dict", "references/rtgtools", "references/rtgtools/genome.sdf", "references/rtgtools/genome.sdf/done", @@ -585,6 +575,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-04T10:10:09.338722844" + "timestamp": "2024-12-10T10:53:32.971378431" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index 5181ca9..9af6025 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'": { "content": [ - 145, + 144, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -30,9 +30,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -82,9 +79,6 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", - "references", - "references/dictionary", - "references/dictionary/genome.dict", "structural", "structural/HG002", "structural/HG002/preprocess", @@ -363,11 +357,11 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-04T10:22:09.959009433" + "timestamp": "2024-12-10T10:42:14.227940401" }, "-stub": { "content": [ - 145, + 144, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -396,9 +390,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -448,9 +439,6 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", - "references", - "references/dictionary", - "references/dictionary/genome.dict", "structural", "structural/HG002", "structural/HG002/preprocess", @@ -668,6 +656,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-04T10:24:27.339868009" + "timestamp": "2024-12-10T10:44:36.4725572" } } \ No newline at end of file diff --git a/tests/liftover_truth.nf.test.snap b/tests/liftover_truth.nf.test.snap index fc319d7..ef7a85a 100644 --- a/tests/liftover_truth.nf.test.snap +++ b/tests/liftover_truth.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover 'truth'": { "content": [ - 86, + 85, { "BCFTOOLS_DEDUP": { "bcftools": 1.18 @@ -33,9 +33,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -354,6 +351,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-03T15:58:16.618582714" + "timestamp": "2024-12-10T10:20:35.930923969" } } \ No newline at end of file diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 2e1d10b..1f04021 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'somatic' --variant_type 'indel' --method 'sompy'": { "content": [ - 28, + 27, { "BCFTOOLS_REHEADER": { "bcftools": 1.18 @@ -30,9 +30,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -168,10 +165,7 @@ "indel/test2/stats/bcftools", "indel/test2/stats/bcftools/test2.strelka.bcftools_stats.txt", "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", - "references", - "references/dictionary", - "references/dictionary/genome.dict" + "pipeline_info/nf_core_pipeline_software_mqc_versions.yml" ], [ "SEQC2.bcftools_stats.txt:md5,e530daf4f6a4923f1cd85c51893d5747", @@ -183,6 +177,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-04T09:59:41.934881268" + "timestamp": "2024-12-10T11:27:48.141030523" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index 539def8..b2ccd3d 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'somatic' --variant_type 'structural' --method 'truvari,svbenchmark'": { "content": [ - 70, + 69, { "BCFTOOLS_REHEADER": { "bcftools": 1.18 @@ -30,9 +30,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -64,9 +61,6 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", - "references", - "references/dictionary", - "references/dictionary/genome.dict", "structural", "structural/SEQC2", "structural/SEQC2/stats", @@ -212,6 +206,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-04T09:56:29.305188123" + "timestamp": "2024-12-10T11:06:49.710866299" } } \ No newline at end of file From 969ecb4924099dbbc6424f8eb50f742b67e04bcf Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 10 Dec 2024 13:34:52 +0000 Subject: [PATCH 230/292] renew snapshot --- conf/tests/somatic_indel.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/tests/somatic_indel.config b/conf/tests/somatic_indel.config index d51111c..67ffe08 100644 --- a/conf/tests/somatic_indel.config +++ b/conf/tests/somatic_indel.config @@ -33,7 +33,7 @@ params { truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sINDEL_truth_set_v1.0.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" } From 789342e6336c54755d65e90b3258b38ffc4fece5 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 10 Dec 2024 13:55:38 +0000 Subject: [PATCH 231/292] add new snapshot --- tests/somatic_snv.nf.test.snap | 10 ++-------- 1 file changed, 2 insertions(+), 8 deletions(-) diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 20188bb..5f03580 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -1,7 +1,7 @@ { "-stub": { "content": [ - 39, + 38, { "BCFTOOLS_REHEADER": { "bcftools": 1.18 @@ -30,9 +30,6 @@ "MERGE_REPORTS": { "python": "3.8.6" }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, "PLOTS": { "r-base": "4.3.1" }, @@ -49,9 +46,6 @@ [ "pipeline_info", "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", - "references", - "references/dictionary", - "references/dictionary/genome.dict", "snv", "snv/SEQC2", "snv/SEQC2/stats", @@ -133,6 +127,6 @@ "nf-test": "0.9.0", "nextflow": "24.10.2" }, - "timestamp": "2024-12-04T09:53:59.901867572" + "timestamp": "2024-12-10T13:54:36.607079161" } } \ No newline at end of file From aecd4566a6ca0e4a4d377e122da6e4eb759677b3 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 12 Dec 2024 11:24:56 +0000 Subject: [PATCH 232/292] Template update for nf-core/tools version 3.1.0 --- .github/CONTRIBUTING.md | 12 +- .github/workflows/awsfulltest.yml | 21 +- .github/workflows/branch.yml | 18 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 8 +- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting.yml | 10 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .../workflows/template_version_comment.yml | 2 +- .gitpod.yml | 11 +- .nf-core.yml | 9 +- .vscode/settings.json | 3 + conf/base.config | 2 +- conf/modules.config | 1 + docs/usage.md | 32 +- modules.json | 8 +- modules/nf-core/fastqc/main.nf | 2 +- modules/nf-core/fastqc/meta.yml | 1 + nextflow.config | 45 ++- nextflow_schema.json | 6 + ro-crate-metadata.json | 295 ++++++++++++++++++ .../main.nf | 7 +- .../nf-core/utils_nextflow_pipeline/main.nf | 2 + .../tests/main.workflow.nf.test | 10 +- .../nf-core/utils_nfcore_pipeline/main.nf | 89 ++---- .../tests/main.function.nf.test | 46 ++- .../tests/main.function.nf.test.snap | 30 -- .../utils_nfschema_plugin/tests/main.nf.test | 4 +- workflows/variantbenchmarking.nf | 2 +- 30 files changed, 467 insertions(+), 219 deletions(-) create mode 100644 .vscode/settings.json create mode 100644 ro-crate-metadata.json diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 37f8c98..a27b873 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -1,4 +1,4 @@ -# nf-core/variantbenchmarking: Contributing Guidelines +# `nf-core/variantbenchmarking`: Contributing Guidelines Hi there! Many thanks for taking an interest in improving nf-core/variantbenchmarking. @@ -55,9 +55,9 @@ These tests are run both with the latest available version of `Nextflow` and als :warning: Only in the unlikely and regretful event of a release happening with a bug. -- On your own fork, make a new branch `patch` based on `upstream/master`. +- On your own fork, make a new branch `patch` based on `upstream/main` or `upstream/master`. - Fix the bug, and bump version (X.Y.Z+1). -- A PR should be made on `master` from patch to directly this particular bug. +- Open a pull-request from `patch` to `main`/`master` with the changes. ## Getting help @@ -65,13 +65,13 @@ For further information/help, please consult the [nf-core/variantbenchmarking do ## Pipeline contribution conventions -To make the nf-core/variantbenchmarking code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. +To make the `nf-core/variantbenchmarking` code and processing logic more understandable for new contributors and to ensure quality, we semi-standardise the way the code and other contributions are written. ### Adding a new step If you wish to contribute a new step, please use the following coding standards: -1. Define the corresponding input channel into your new process from the expected previous process channel +1. Define the corresponding input channel into your new process from the expected previous process channel. 2. Write the process block (see below). 3. Define the output channel if needed (see below). 4. Add any new parameters to `nextflow.config` with a default (see below). @@ -84,7 +84,7 @@ If you wish to contribute a new step, please use the following coding standards: ### Default values -Parameters should be initialised / defined with default values in `nextflow.config` under the `params` scope. +Parameters should be initialised / defined with default values within the `params` scope in `nextflow.config`. Once there, use `nf-core pipelines schema build` to add to `nextflow_schema.json`. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 6289350..9b0eeaf 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -1,11 +1,12 @@ name: nf-core AWS full size tests -# This workflow is triggered on PRs opened against the master branch. +# This workflow is triggered on PRs opened against the main/master branch. # It can be additionally triggered manually with GitHub actions workflow dispatch button. # It runs the -profile 'test_full' on AWS batch on: pull_request: branches: + - main - master workflow_dispatch: pull_request_review: @@ -18,18 +19,30 @@ jobs: if: github.repository == 'nf-core/variantbenchmarking' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch' runs-on: ubuntu-latest steps: - - uses: octokit/request-action@v2.x + - name: Get PR reviews + uses: octokit/request-action@v2.x + if: github.event_name != 'workflow_dispatch' id: check_approvals + continue-on-error: true with: - route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews + route: GET /repos/${{ github.repository }}/pulls/${{ github.event.pull_request.number }}/reviews?per_page=100 env: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} - - id: test_variables + + - name: Check for approvals + if: ${{ failure() && github.event_name != 'workflow_dispatch' }} + run: | + echo "No review approvals found. At least 2 approvals are required to run this action automatically." + exit 1 + + - name: Check for enough approvals (>=2) + id: test_variables if: github.event_name != 'workflow_dispatch' run: | JSON_RESPONSE='${{ steps.check_approvals.outputs.data }}' CURRENT_APPROVALS_COUNT=$(echo $JSON_RESPONSE | jq -c '[.[] | select(.state | contains("APPROVED")) ] | length') test $CURRENT_APPROVALS_COUNT -ge 2 || exit 1 # At least 2 approvals are required + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 58404c3..9d2c4e6 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -1,15 +1,17 @@ name: nf-core branch protection -# This workflow is triggered on PRs to master branch on the repository -# It fails when someone tries to make a PR against the nf-core `master` branch instead of `dev` +# This workflow is triggered on PRs to `main`/`master` branch on the repository +# It fails when someone tries to make a PR against the nf-core `main`/`master` branch instead of `dev` on: pull_request_target: - branches: [master] + branches: + - main + - master jobs: test: runs-on: ubuntu-latest steps: - # PRs to the nf-core repo master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches + # PRs to the nf-core repo main/master branch are only ok if coming from the nf-core repo `dev` or any `patch` branches - name: Check PRs if: github.repository == 'nf-core/variantbenchmarking' run: | @@ -22,7 +24,7 @@ jobs: uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | - ## This PR is against the `master` branch :x: + ## This PR is against the `${{github.event.pull_request.base.ref}}` branch :x: * Do not close this PR * Click _Edit_ and change the `base` to `dev` @@ -32,9 +34,9 @@ jobs: Hi @${{ github.event.pull_request.user.login }}, - It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `master` branch. - The `master` branch on nf-core repositories should always contain code from the latest release. - Because of this, PRs to `master` are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch. + It looks like this pull-request is has been made against the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) ${{github.event.pull_request.base.ref}} branch. + The ${{github.event.pull_request.base.ref}} branch on nf-core repositories should always contain code from the latest release. + Because of this, PRs to ${{github.event.pull_request.base.ref}} are only allowed if they come from the [${{github.event.pull_request.head.repo.full_name }}](https://github.com/${{github.event.pull_request.head.repo.full_name }}) `dev` branch. You do not need to close this PR, you can change the target branch to `dev` by clicking the _"Edit"_ button at the top of this page. Note that even after this, the test will continue to show as failing until you push a new commit. diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index c599817..ef4cd4d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -45,7 +45,7 @@ jobs: profile: "singularity" steps: - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4 - name: Set up Nextflow uses: nf-core/setup-nextflow@v2 diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 713dc3e..2576cc0 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -2,7 +2,7 @@ name: Test successful pipeline download with 'nf-core pipelines download' # Run the workflow when: # - dispatched manually -# - when a PR is opened or reopened to master branch +# - when a PR is opened or reopened to main/master branch # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: @@ -17,9 +17,11 @@ on: - edited - synchronize branches: + - main - master pull_request_target: branches: + - main - master env: @@ -35,7 +37,7 @@ jobs: - name: Disk space cleanup uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5 with: python-version: "3.12" architecture: "x64" @@ -69,7 +71,7 @@ jobs: --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ --container-system 'singularity' \ - --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io" \ + --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io/library/" \ --container-cache-utilisation 'amend' \ --download-configuration 'yes' diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 4d278c6..56988a5 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -32,7 +32,7 @@ jobs: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} # Install and run pre-commit - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5 with: python-version: "3.12" diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index a502573..dbd52d5 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + - uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4 - name: Set up Python 3.12 - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5 with: python-version: "3.12" @@ -31,12 +31,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4 - name: Install Nextflow uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5 with: python-version: "3.12" architecture: "x64" @@ -74,7 +74,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 + uses: actions/upload-artifact@b4b15b8c7c6ac21ea08fcf65892d2ee8f75cf882 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 42e519b..0bed96d 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@bf251b5aa9c2f7eeb574a96ee720e24f801b7c11 # v6 + uses: dawidd6/action-download-artifact@80620a5d27ce0ae443b965134db88467fc607b43 # v7 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c6ba35d..450b1d5 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -31,7 +31,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 + - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5 with: python-version: "3.10" - name: Install dependencies diff --git a/.github/workflows/template_version_comment.yml b/.github/workflows/template_version_comment.yml index e8aafe4..537529b 100644 --- a/.github/workflows/template_version_comment.yml +++ b/.github/workflows/template_version_comment.yml @@ -9,7 +9,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 + uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4 with: ref: ${{ github.event.pull_request.head.sha }} diff --git a/.gitpod.yml b/.gitpod.yml index 4611863..83599f6 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -6,12 +6,5 @@ tasks: nextflow self-update vscode: - extensions: # based on nf-core.nf-core-extensionpack - #- esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - - nextflow.nextflow # Nextflow syntax highlighting - - oderwat.indent-rainbow # Highlight indentation level - - streetsidesoftware.code-spell-checker # Spelling checker for source code - - charliermarsh.ruff # Code linter Ruff + extensions: + - nf-core.nf-core-extensionpack # https://github.com/nf-core/vscode-extensionpack diff --git a/.nf-core.yml b/.nf-core.yml index 7ab9250..497b269 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,7 +1,6 @@ -bump_version: null -lint: null -nf_core_version: 3.0.2 -org_path: null +lint: + actions_ci: false +nf_core_version: 3.1.0 repository_type: pipeline template: author: kuebra.narci@dkfz.de @@ -11,6 +10,4 @@ template: name: variantbenchmarking org: nf-core outdir: . - skip_features: null version: 1.0dev -update: null diff --git a/.vscode/settings.json b/.vscode/settings.json new file mode 100644 index 0000000..a33b527 --- /dev/null +++ b/.vscode/settings.json @@ -0,0 +1,3 @@ +{ + "markdown.styles": ["public/vscode_markdown.css"] +} diff --git a/conf/base.config b/conf/base.config index 135e755..f5c60d6 100644 --- a/conf/base.config +++ b/conf/base.config @@ -20,7 +20,7 @@ process { maxErrors = '-1' // Process-specific resource requirements - // NOTE - Please try and re-use the labels below as much as possible. + // NOTE - Please try and reuse the labels below as much as possible. // These labels are used and recognised by default in DSL2 files hosted on nf-core/modules. // If possible, it would be nice to keep the same label naming convention when // adding in your local modules too. diff --git a/conf/modules.config b/conf/modules.config index d266a38..d203d2b 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -21,6 +21,7 @@ process { withName: FASTQC { ext.args = '--quiet' } + withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/docs/usage.md b/docs/usage.md index c7a679e..c571a22 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -75,9 +75,8 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. -:::warning -Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). -::: +> [!WARNING] +> Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). The above pipeline run specified with a params file in yaml format: @@ -106,23 +105,21 @@ nextflow pull nf-core/variantbenchmarking ### Reproducibility -It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. +It is a good idea to specify the pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. First, go to the [nf-core/variantbenchmarking releases page](https://github.com/nf-core/variantbenchmarking/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. -To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. +To further assist in reproducibility, you can use share and reuse [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. -:::tip -If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. -::: +> [!TIP] +> If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. ## Core Nextflow arguments -:::note -These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). -::: +> [!NOTE] +> These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen) ### `-profile` @@ -130,16 +127,15 @@ Use this parameter to choose a configuration profile. Profiles can give configur Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. -:::info -We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. -::: +> [!IMPORTANT] +> We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. -The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to see if your system is available in these configs please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). +The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is suported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! They are loaded in sequence, so later profiles can overwrite earlier profiles. -If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer enviroment. +If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer environment. - `test` - A profile with a complete configuration for automated testing @@ -175,13 +171,13 @@ Specify the path to a specific config file (this is a core Nextflow command). Se ### Resource requests -Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. +Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the pipeline steps, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher resources request (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website. ### Custom Containers -In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date. +In some cases, you may wish to change the container or conda environment used by a pipeline steps for a particular tool. By default, nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However, in some cases the pipeline specified version maybe out of date. To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website. diff --git a/modules.json b/modules.json index accb0d3..ce29ad9 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", + "git_sha": "dc94b6ee04a05ddb9f7ae050712ff30a13149164", "installed_by": ["modules"] }, "multiqc": { @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "3aa0aec1d52d492fe241919f0c6100ebf0074082", + "git_sha": "c2b22d85f30a706a3073387f30380704fcae013b", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "1b6b9a3338d011367137808b49b923515080e3ba", + "git_sha": "51ae5406a030d4da1e49e4dab49756844fdd6c7a", "installed_by": ["subworkflows"] }, "utils_nfschema_plugin": { "branch": "master", - "git_sha": "bbd5a41f4535a8defafe6080e00ea74c45f4f96c", + "git_sha": "2fd2cd6d0e7b273747f32e465fdc6bcc3ae0814e", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index d8989f4..752c3a1 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -24,7 +24,7 @@ process FASTQC { // Make list of old name and new name pairs to use for renaming in the bash while loop def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } def rename_to = old_new_pairs*.join(' ').join(' ') - def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + def renamed_files = old_new_pairs.collect{ _old_name, new_name -> new_name }.join(' ') // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory) // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222 diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4827da7..2b2e62b 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -11,6 +11,7 @@ tools: FastQC gives general quality metrics about your reads. It provides information about the quality score distribution across your reads, the per base sequence content (%A/C/G/T). + You get information about adapter contamination and other overrepresented sequences. homepage: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/ diff --git a/nextflow.config b/nextflow.config index c89633f..894251a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -38,8 +38,7 @@ params { show_hidden = false version = false pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' - - // Config options + trace_report_suffix = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss')// Config options config_profile_name = null config_profile_description = null @@ -153,6 +152,13 @@ profiles { executor.name = 'local' executor.cpus = 4 executor.memory = 8.GB + process { + resourceLimits = [ + memory: 8.GB, + cpus : 4, + time : 1.h + ] + } } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } @@ -201,30 +207,41 @@ set -C # No clobber - prevent output redirection from overwriting files. // Disable process selector warnings by default. Use debug profile to enable warnings. nextflow.enable.configProcessNamesValidation = false -def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true - file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/execution_timeline_${params.trace_report_suffix}.html" } report { enabled = true - file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/execution_report_${params.trace_report_suffix}.html" } trace { enabled = true - file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt" + file = "${params.outdir}/pipeline_info/execution_trace_${params.trace_report_suffix}.txt" } dag { enabled = true - file = "${params.outdir}/pipeline_info/pipeline_dag_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/pipeline_dag_${params.trace_report_suffix}.html" } manifest { name = 'nf-core/variantbenchmarking' - author = """kuebra.narci@dkfz.de""" + author = """kuebra.narci@dkfz.de""" // The author field is deprecated from Nextflow version 24.10.0, use contributors instead + contributors = [ + // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0 + [ + name: 'kuebra.narci@dkfz.de', + affiliation: '', + email: '', + github: '', + contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor') + orcid: '' + ], + ] homePage = 'https://github.com/nf-core/variantbenchmarking' description = """Benchmarking pipeline for germline and somatic variant callers """ mainScript = 'main.nf' + defaultBranch = 'master' nextflowVersion = '!>=24.04.2' version = '1.0dev' doi = '' @@ -237,9 +254,10 @@ plugins { validation { defaultIgnoreParams = ["genomes"] + monochromeLogs = params.monochrome_logs help { enabled = true - command = "nextflow run $manifest.name -profile --input samplesheet.csv --outdir " + command = "nextflow run nf-core/variantbenchmarking -profile --input samplesheet.csv --outdir " fullParameter = "help_full" showHiddenParameter = "show_hidden" beforeText = """ @@ -249,15 +267,15 @@ validation { \033[0;34m |\\ | |__ __ / ` / \\ |__) |__ \033[0;33m} {\033[0m \033[0;34m | \\| | \\__, \\__/ | \\ |___ \033[0;32m\\`-._,-`-,\033[0m \033[0;32m`._,._,\'\033[0m -\033[0;35m ${manifest.name} ${manifest.version}\033[0m +\033[0;35m nf-core/variantbenchmarking ${manifest.version}\033[0m -\033[2m----------------------------------------------------\033[0m- """ - afterText = """${manifest.doi ? "* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { " https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""} + afterText = """${manifest.doi ? "\n* The pipeline\n" : ""}${manifest.doi.tokenize(",").collect { " https://doi.org/${it.trim().replace('https://doi.org/','')}"}.join("\n")}${manifest.doi ? "\n" : ""} * The nf-core framework https://doi.org/10.1038/s41587-020-0439-x * Software dependencies - https://github.com/${manifest.name}/blob/master/CITATIONS.md + https://github.com/nf-core/variantbenchmarking/blob/master/CITATIONS.md """ } summary { @@ -265,6 +283,3 @@ validation { afterText = validation.help.afterText } } - -// Load modules.config for DSL2 module specific options -includeConfig 'conf/modules.config' diff --git a/nextflow_schema.json b/nextflow_schema.json index e98f7f5..024ba00 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -218,6 +218,12 @@ "description": "Base URL or local path to location of pipeline test dataset files", "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", "hidden": true + }, + "trace_report_suffix": { + "type": "string", + "fa_icon": "far calendar", + "description": "Suffix to add to the trace report filename. Default is the date and time in the format yyyy-MM-dd_HH-mm-ss.", + "hidden": true } } } diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json new file mode 100644 index 0000000..05744f7 --- /dev/null +++ b/ro-crate-metadata.json @@ -0,0 +1,295 @@ +{ + "@context": [ + "https://w3id.org/ro/crate/1.1/context", + { + "GithubService": "https://w3id.org/ro/terms/test#GithubService", + "JenkinsService": "https://w3id.org/ro/terms/test#JenkinsService", + "PlanemoEngine": "https://w3id.org/ro/terms/test#PlanemoEngine", + "TestDefinition": "https://w3id.org/ro/terms/test#TestDefinition", + "TestInstance": "https://w3id.org/ro/terms/test#TestInstance", + "TestService": "https://w3id.org/ro/terms/test#TestService", + "TestSuite": "https://w3id.org/ro/terms/test#TestSuite", + "TravisService": "https://w3id.org/ro/terms/test#TravisService", + "definition": "https://w3id.org/ro/terms/test#definition", + "engineVersion": "https://w3id.org/ro/terms/test#engineVersion", + "instance": "https://w3id.org/ro/terms/test#instance", + "resource": "https://w3id.org/ro/terms/test#resource", + "runsOn": "https://w3id.org/ro/terms/test#runsOn" + } + ], + "@graph": [ + { + "@id": "./", + "@type": "Dataset", + "creativeWorkStatus": "InProgress", + "datePublished": "2024-12-12T11:24:53+00:00", + "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    \n\n[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is a bioinformatics pipeline that ...\n\n\n\n\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n\n\nNow, you can run the pipeline using:\n\n\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", + "hasPart": [ + { + "@id": "main.nf" + }, + { + "@id": "assets/" + }, + { + "@id": "conf/" + }, + { + "@id": "docs/" + }, + { + "@id": "docs/images/" + }, + { + "@id": "modules/" + }, + { + "@id": "modules/nf-core/" + }, + { + "@id": "workflows/" + }, + { + "@id": "subworkflows/" + }, + { + "@id": "nextflow.config" + }, + { + "@id": "README.md" + }, + { + "@id": "nextflow_schema.json" + }, + { + "@id": "CHANGELOG.md" + }, + { + "@id": "LICENSE" + }, + { + "@id": "CODE_OF_CONDUCT.md" + }, + { + "@id": "CITATIONS.md" + }, + { + "@id": "modules.json" + }, + { + "@id": "docs/usage.md" + }, + { + "@id": "docs/output.md" + }, + { + "@id": ".nf-core.yml" + }, + { + "@id": ".pre-commit-config.yaml" + }, + { + "@id": ".prettierignore" + } + ], + "isBasedOn": "https://github.com/nf-core/variantbenchmarking", + "license": "MIT", + "mainEntity": { + "@id": "main.nf" + }, + "mentions": [ + { + "@id": "#d1132ae2-a4ec-465b-adcb-386595845ac2" + } + ], + "name": "nf-core/variantbenchmarking" + }, + { + "@id": "ro-crate-metadata.json", + "@type": "CreativeWork", + "about": { + "@id": "./" + }, + "conformsTo": [ + { + "@id": "https://w3id.org/ro/crate/1.1" + }, + { + "@id": "https://w3id.org/workflowhub/workflow-ro-crate/1.0" + } + ] + }, + { + "@id": "main.nf", + "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"], + "dateCreated": "", + "dateModified": "2024-12-12T11:24:53Z", + "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/", + "keywords": ["nf-core", "nextflow", "benchmark", "draft", "structural-variants", "variant-calling"], + "license": ["MIT"], + "name": ["nf-core/variantbenchmarking"], + "programmingLanguage": { + "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow" + }, + "sdPublisher": { + "@id": "https://nf-co.re/" + }, + "url": ["https://github.com/nf-core/variantbenchmarking", "https://nf-co.re/variantbenchmarking/dev/"], + "version": ["1.0dev"] + }, + { + "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow", + "@type": "ComputerLanguage", + "identifier": { + "@id": "https://www.nextflow.io/" + }, + "name": "Nextflow", + "url": { + "@id": "https://www.nextflow.io/" + }, + "version": "!>=24.04.2" + }, + { + "@id": "#d1132ae2-a4ec-465b-adcb-386595845ac2", + "@type": "TestSuite", + "instance": [ + { + "@id": "#5b1edf7d-b297-4c18-aff3-1adbc98ced26" + } + ], + "mainEntity": { + "@id": "main.nf" + }, + "name": "Test suite for nf-core/variantbenchmarking" + }, + { + "@id": "#5b1edf7d-b297-4c18-aff3-1adbc98ced26", + "@type": "TestInstance", + "name": "GitHub Actions workflow for testing nf-core/variantbenchmarking", + "resource": "repos/nf-core/variantbenchmarking/actions/workflows/ci.yml", + "runsOn": { + "@id": "https://w3id.org/ro/terms/test#GithubService" + }, + "url": "https://api.github.com" + }, + { + "@id": "https://w3id.org/ro/terms/test#GithubService", + "@type": "TestService", + "name": "Github Actions", + "url": { + "@id": "https://github.com" + } + }, + { + "@id": "assets/", + "@type": "Dataset", + "description": "Additional files" + }, + { + "@id": "conf/", + "@type": "Dataset", + "description": "Configuration files" + }, + { + "@id": "docs/", + "@type": "Dataset", + "description": "Markdown files for documenting the pipeline" + }, + { + "@id": "docs/images/", + "@type": "Dataset", + "description": "Images for the documentation files" + }, + { + "@id": "modules/", + "@type": "Dataset", + "description": "Modules used by the pipeline" + }, + { + "@id": "modules/nf-core/", + "@type": "Dataset", + "description": "nf-core modules" + }, + { + "@id": "workflows/", + "@type": "Dataset", + "description": "Main pipeline workflows to be executed in main.nf" + }, + { + "@id": "subworkflows/", + "@type": "Dataset", + "description": "Smaller subworkflows" + }, + { + "@id": "nextflow.config", + "@type": "File", + "description": "Main Nextflow configuration file" + }, + { + "@id": "README.md", + "@type": "File", + "description": "Basic pipeline usage information" + }, + { + "@id": "nextflow_schema.json", + "@type": "File", + "description": "JSON schema for pipeline parameter specification" + }, + { + "@id": "CHANGELOG.md", + "@type": "File", + "description": "Information on changes made to the pipeline" + }, + { + "@id": "LICENSE", + "@type": "File", + "description": "The license - should be MIT" + }, + { + "@id": "CODE_OF_CONDUCT.md", + "@type": "File", + "description": "The nf-core code of conduct" + }, + { + "@id": "CITATIONS.md", + "@type": "File", + "description": "Citations needed when using the pipeline" + }, + { + "@id": "modules.json", + "@type": "File", + "description": "Version information for modules from nf-core/modules" + }, + { + "@id": "docs/usage.md", + "@type": "File", + "description": "Usage documentation" + }, + { + "@id": "docs/output.md", + "@type": "File", + "description": "Output documentation" + }, + { + "@id": ".nf-core.yml", + "@type": "File", + "description": "nf-core configuration file, configuring template features and linting rules" + }, + { + "@id": ".pre-commit-config.yaml", + "@type": "File", + "description": "Configuration file for pre-commit hooks" + }, + { + "@id": ".prettierignore", + "@type": "File", + "description": "Ignore file for prettier" + }, + { + "@id": "https://nf-co.re/", + "@type": "Organization", + "name": "nf-core", + "url": "https://nf-co.re/" + } + ] +} diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index fa469fa..af5e47b 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -116,7 +116,8 @@ workflow PIPELINE_COMPLETION { main: summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") - + def multiqc_reports = multiqc_report.toList() + // // Completion email and summary // @@ -129,7 +130,7 @@ workflow PIPELINE_COMPLETION { plaintext_email, outdir, monochrome_logs, - multiqc_report.toList() + multiqc_reports.getVal(), ) } @@ -225,7 +226,7 @@ def toolBibliographyText() { } def methodsDescriptionText(mqc_methods_yaml) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + // Convert to a named map so can be used as with familiar NXF ${workflow} variable syntax in the MultiQC YML file def meta = [:] meta.workflow = workflow.toMap() meta["manifest_map"] = workflow.manifest.toMap() diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf index 0fcbf7b..d6e593e 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -92,10 +92,12 @@ def checkCondaChannels() { channels = config.channels } catch (NullPointerException e) { + log.debug(e) log.warn("Could not verify conda channel configuration.") return null } catch (IOException e) { + log.debug(e) log.warn("Could not verify conda channel configuration.") return null } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index ca964ce..02dbf09 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -52,10 +52,12 @@ nextflow_workflow { } then { - assertAll( - { assert workflow.success }, - { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } - ) + expect { + with(workflow) { + assert success + assert "nextflow_workflow v9.9.9" in stdout + } + } } } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index 5cb7baf..bfd2587 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -56,21 +56,6 @@ def checkProfileProvided(nextflow_cli_args) { } } -// -// Citation string for pipeline -// -def workflowCitation() { - def temp_doi_ref = "" - def manifest_doi = workflow.manifest.doi.tokenize(",") - // Handling multiple DOIs - // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers - // Removing ` ` since the manifest.doi is a string and not a proper list - manifest_doi.each { doi_ref -> - temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" - } - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" -} - // // Generate workflow version string // @@ -150,33 +135,6 @@ def paramsSummaryMultiqc(summary_params) { return yaml_file_text } -// -// nf-core logo -// -def nfCoreLogo(monochrome_logs=true) { - def colors = logColours(monochrome_logs) as Map - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) -} - -// -// Return dashed line -// -def dashedLine(monochrome_logs=true) { - def colors = logColours(monochrome_logs) as Map - return "-${colors.dim}----------------------------------------------------${colors.reset}-" -} - // // ANSII colours used for terminal logging // @@ -245,28 +203,24 @@ def logColours(monochrome_logs=true) { return colorcodes } -// -// Attach the multiqc report to email -// -def attachMultiqcReport(multiqc_report) { - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one") - } - mqc_report = mqc_report[0] - } +// Return a single report from an object that may be a Path or List +// +def getSingleReport(multiqc_reports) { + if (multiqc_reports instanceof Path) { + return multiqc_reports + } else if (multiqc_reports instanceof List) { + if (multiqc_reports.size() == 0) { + log.warn("[${workflow.manifest.name}] No reports found from process 'MULTIQC'") + return null + } else if (multiqc_reports.size() == 1) { + return multiqc_reports.first() + } else { + log.warn("[${workflow.manifest.name}] Found multiple reports from process 'MULTIQC', will use only one") + return multiqc_reports.first() } + } else { + return null } - catch (Exception all) { - if (multiqc_report) { - log.warn("[${workflow.manifest.name}] Could not attach MultiQC report to summary email") - } - } - return mqc_report } // @@ -320,7 +274,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi email_fields['summary'] = summary << misc_fields // On success try attach the multiqc report - def mqc_report = attachMultiqcReport(multiqc_report) + def mqc_report = getSingleReport(multiqc_report) // Check if we are only sending emails on failure def email_address = email @@ -340,7 +294,7 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi def email_html = html_template.toString() // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as MemoryUnit def smail_fields = [email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes()] def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) @@ -351,14 +305,17 @@ def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdi if (email_address) { try { if (plaintext_email) { -new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') } + new org.codehaus.groovy.GroovyException('Send plaintext e-mail, not HTML') + } // Try to send HTML e-mail using sendmail def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") sendmail_tf.withWriter { w -> w << sendmail_html } ['sendmail', '-t'].execute() << sendmail_html log.info("-${colors.purple}[${workflow.manifest.name}]${colors.green} Sent summary e-mail to ${email_address} (sendmail)-") } - catch (Exception all) { + catch (Exception msg) { + log.debug(msg.toString()) + log.debug("Trying with mail instead of sendmail") // Catch failures and try with plaintext def mail_cmd = ['mail', '-s', subject, '--content-type=text/html', email_address] mail_cmd.execute() << email_html diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test index 1dc317f..f117040 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -41,26 +41,14 @@ nextflow_function { } } - test("Test Function workflowCitation") { - - function "workflowCitation" - - then { - assertAll( - { assert function.success }, - { assert snapshot(function.result).match() } - ) - } - } - - test("Test Function nfCoreLogo") { + test("Test Function without logColours") { - function "nfCoreLogo" + function "logColours" when { function { """ - input[0] = false + input[0] = true """ } } @@ -73,9 +61,8 @@ nextflow_function { } } - test("Test Function dashedLine") { - - function "dashedLine" + test("Test Function with logColours") { + function "logColours" when { function { @@ -93,14 +80,13 @@ nextflow_function { } } - test("Test Function without logColours") { - - function "logColours" + test("Test Function getSingleReport with a single file") { + function "getSingleReport" when { function { """ - input[0] = true + input[0] = file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true) """ } } @@ -108,18 +94,22 @@ nextflow_function { then { assertAll( { assert function.success }, - { assert snapshot(function.result).match() } + { assert function.result.contains("test.tsv") } ) } } - test("Test Function with logColours") { - function "logColours" + test("Test Function getSingleReport with multiple files") { + function "getSingleReport" when { function { """ - input[0] = false + input[0] = [ + file(params.modules_testdata_base_path + '/generic/tsv/test.tsv', checkIfExists: true), + file(params.modules_testdata_base_path + '/generic/tsv/network.tsv', checkIfExists: true), + file(params.modules_testdata_base_path + '/generic/tsv/expression.tsv', checkIfExists: true) + ] """ } } @@ -127,7 +117,9 @@ nextflow_function { then { assertAll( { assert function.success }, - { assert snapshot(function.result).match() } + { assert function.result.contains("test.tsv") }, + { assert !function.result.contains("network.tsv") }, + { assert !function.result.contains("expression.tsv") } ) } } diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 1037232..02c6701 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -17,26 +17,6 @@ }, "timestamp": "2024-02-28T12:02:59.729647" }, - "Test Function nfCoreLogo": { - "content": [ - "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-28T12:03:10.562934" - }, - "Test Function workflowCitation": { - "content": [ - "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-28T12:03:07.019761" - }, "Test Function without logColours": { "content": [ { @@ -95,16 +75,6 @@ }, "timestamp": "2024-02-28T12:03:17.969323" }, - "Test Function dashedLine": { - "content": [ - "-\u001b[2m----------------------------------------------------\u001b[0m-" - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-02-28T12:03:14.366181" - }, "Test Function with logColours": { "content": [ { diff --git a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test index 842dc43..8fb3016 100644 --- a/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfschema_plugin/tests/main.nf.test @@ -42,7 +42,7 @@ nextflow_workflow { params { test_data = '' - outdir = 1 + outdir = null } workflow { @@ -94,7 +94,7 @@ nextflow_workflow { params { test_data = '' - outdir = 1 + outdir = null } workflow { diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 0ba2091..c9f40a7 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -39,7 +39,7 @@ workflow VARIANTBENCHMARKING { softwareVersionsToYAML(ch_versions) .collectFile( storeDir: "${params.outdir}/pipeline_info", - name: 'nf_core_' + 'pipeline_software_' + 'mqc_' + 'versions.yml', + name: 'nf_core_' + 'variantbenchmarking_software_' + 'mqc_' + 'versions.yml', sort: true, newLine: true ).set { ch_collated_versions } From 0104f0b4ccf50f0d402a22e4909eefd419f25d63 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 20 Dec 2024 13:11:00 +0000 Subject: [PATCH 233/292] Template update for nf-core/tools version 3.1.1 --- .editorconfig | 4 ++ .github/ISSUE_TEMPLATE/bug_report.yml | 1 - .github/workflows/download_pipeline.yml | 41 +++++++++------- .nf-core.yml | 2 +- .prettierignore | 1 + CITATIONS.md | 4 +- LICENSE | 2 +- README.md | 15 ++---- docs/output.md | 11 ++--- docs/usage.md | 2 +- nextflow.config | 3 ++ ro-crate-metadata.json | 48 +++++++++++++------ .../main.nf | 2 +- 13 files changed, 79 insertions(+), 57 deletions(-) diff --git a/.editorconfig b/.editorconfig index 72dda28..6d9b74c 100644 --- a/.editorconfig +++ b/.editorconfig @@ -31,3 +31,7 @@ indent_size = unset # ignore python and markdown [*.{py,md}] indent_style = unset + +# ignore ro-crate metadata files +[**/ro-crate-metadata.json] +insert_final_newline = unset diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 094f013..7bcf0bb 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -9,7 +9,6 @@ body: - [nf-core website: troubleshooting](https://nf-co.re/usage/troubleshooting) - [nf-core/variantbenchmarking pipeline documentation](https://nf-co.re/variantbenchmarking/usage) - - type: textarea id: description attributes: diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 2576cc0..13b51e2 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,8 +28,12 @@ env: NXF_ANSI_LOG: false jobs: - download: + configure: runs-on: ubuntu-latest + outputs: + REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }} + REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }} + REPO_BRANCH: ${{ steps.get_repo_properties.outputs.REPO_BRANCH }} steps: - name: Install Nextflow uses: nf-core/setup-nextflow@v2 @@ -53,22 +57,27 @@ jobs: pip install git+https://github.com/nf-core/tools.git@dev - name: Get the repository name and current branch set as environment variable + id: get_repo_properties run: | - echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} - echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT" + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT" + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT" - name: Make a cache directory for the container images run: | mkdir -p ./singularity_container_images + download: + runs-on: ubuntu-latest + needs: configure + steps: - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./singularity_container_images run: | - nf-core pipelines download ${{ env.REPO_LOWERCASE }} \ - --revision ${{ env.REPO_BRANCH }} \ - --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + nf-core pipelines download ${{ needs.configure.outputs.REPO_LOWERCASE }} \ + --revision ${{ needs.configure.outputs.REPO_BRANCH }} \ + --outdir ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} \ --compress "none" \ --container-system 'singularity' \ --container-library "quay.io" -l "docker.io" -l "community.wave.seqera.io/library/" \ @@ -76,14 +85,14 @@ jobs: --download-configuration 'yes' - name: Inspect download - run: tree ./${{ env.REPOTITLE_LOWERCASE }} + run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} - name: Count the downloaded number of container images id: count_initial run: | image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) echo "Initial container image count: $image_count" - echo "IMAGE_COUNT_INITIAL=$image_count" >> ${GITHUB_ENV} + echo "IMAGE_COUNT_INITIAL=$image_count" >> "$GITHUB_OUTPUT" - name: Run the downloaded pipeline (stub) id: stub_run_pipeline @@ -91,27 +100,27 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results - name: Run the downloaded pipeline (stub run not supported) id: run_pipeline - if: ${{ job.steps.stub_run_pipeline.status == failure() }} + if: ${{ steps.stub_run_pipeline.outcome == 'failure' }} env: NXF_SINGULARITY_CACHEDIR: ./singularity_container_images NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results + run: nextflow run ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ needs.configure.outputs.REPO_BRANCH }}) -profile test,singularity --outdir ./results - name: Count the downloaded number of container images id: count_afterwards run: | image_count=$(ls -1 ./singularity_container_images | wc -l | xargs) echo "Post-pipeline run container image count: $image_count" - echo "IMAGE_COUNT_AFTER=$image_count" >> ${GITHUB_ENV} + echo "IMAGE_COUNT_AFTER=$image_count" >> "$GITHUB_OUTPUT" - name: Compare container image counts run: | - if [ "${{ env.IMAGE_COUNT_INITIAL }}" -ne "${{ env.IMAGE_COUNT_AFTER }}" ]; then - initial_count=${{ env.IMAGE_COUNT_INITIAL }} - final_count=${{ env.IMAGE_COUNT_AFTER }} + if [ "${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}" -ne "${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}" ]; then + initial_count=${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }} + final_count=${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }} difference=$((final_count - initial_count)) echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!" tree ./singularity_container_images diff --git a/.nf-core.yml b/.nf-core.yml index 497b269..148d2aa 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,6 @@ lint: actions_ci: false -nf_core_version: 3.1.0 +nf_core_version: 3.1.1 repository_type: pipeline template: author: kuebra.narci@dkfz.de diff --git a/.prettierignore b/.prettierignore index 437d763..edd29f0 100644 --- a/.prettierignore +++ b/.prettierignore @@ -10,3 +10,4 @@ testing/ testing* *.pyc bin/ +ro-crate-metadata.json diff --git a/CITATIONS.md b/CITATIONS.md index 44ca0a0..4d44a0c 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,9 +12,7 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) -> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - -- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) +> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. diff --git a/LICENSE b/LICENSE index 0aefc2e..d9ef4e5 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) kuebra.narci@dkfz.de +Copyright (c) The nf-core/variantbenchmarking team Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index 13a69bf..cba9213 100644 --- a/README.md +++ b/README.md @@ -3,9 +3,7 @@ nf-core/variantbenchmarking - - -[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml) +[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) @@ -29,15 +27,12 @@ - - -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) -2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) +1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) ## Usage > [!NOTE] -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - - - + An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. diff --git a/docs/output.md b/docs/output.md index eb14937..228b547 100644 --- a/docs/output.md +++ b/docs/output.md @@ -12,8 +12,7 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC -- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +- [FastQC](#fastqc) - Raw read QC- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ### FastQC @@ -27,9 +26,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
    -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). - -### MultiQC +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).### MultiQC
    Output files @@ -43,9 +40,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . - -### Pipeline information +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see .### Pipeline information
    Output files diff --git a/docs/usage.md b/docs/usage.md index c571a22..01c9245 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -130,7 +130,7 @@ Several generic profiles are bundled with the pipeline which instruct the pipeli > [!IMPORTANT] > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. -The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is suported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). +The pipeline also dynamically loads configurations from [https://github.com/nf-core/configs](https://github.com/nf-core/configs) when it runs, making multiple config profiles for various institutional clusters available at run time. For more information and to check if your system is supported, please see the [nf-core/configs documentation](https://github.com/nf-core/configs#documentation). Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! They are loaded in sequence, so later profiles can overwrite earlier profiles. diff --git a/nextflow.config b/nextflow.config index 894251a..d7b17cf 100644 --- a/nextflow.config +++ b/nextflow.config @@ -283,3 +283,6 @@ validation { afterText = validation.help.afterText } } + +// Load modules.config for DSL2 module specific options +includeConfig 'conf/modules.config' diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json index 05744f7..b7ccc99 100644 --- a/ro-crate-metadata.json +++ b/ro-crate-metadata.json @@ -22,8 +22,8 @@ "@id": "./", "@type": "Dataset", "creativeWorkStatus": "InProgress", - "datePublished": "2024-12-12T11:24:53+00:00", - "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    \n\n[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is a bioinformatics pipeline that ...\n\n\n\n\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))\n2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n\n\nNow, you can run the pipeline using:\n\n\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", + "datePublished": "2024-12-20T13:10:59+00:00", + "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    [![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is a bioinformatics pipeline that ...\n\n\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n\n\nNow, you can run the pipeline using:\n\n\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", "hasPart": [ { "@id": "main.nf" @@ -99,7 +99,7 @@ }, "mentions": [ { - "@id": "#d1132ae2-a4ec-465b-adcb-386595845ac2" + "@id": "#91c4f0d3-aae1-4ff3-b0fc-040d23c0493d" } ], "name": "nf-core/variantbenchmarking" @@ -121,21 +121,41 @@ }, { "@id": "main.nf", - "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"], + "@type": [ + "File", + "SoftwareSourceCode", + "ComputationalWorkflow" + ], "dateCreated": "", - "dateModified": "2024-12-12T11:24:53Z", + "dateModified": "2024-12-20T13:10:59Z", "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/", - "keywords": ["nf-core", "nextflow", "benchmark", "draft", "structural-variants", "variant-calling"], - "license": ["MIT"], - "name": ["nf-core/variantbenchmarking"], + "keywords": [ + "nf-core", + "nextflow", + "benchmark", + "draft", + "structural-variants", + "variant-calling" + ], + "license": [ + "MIT" + ], + "name": [ + "nf-core/variantbenchmarking" + ], "programmingLanguage": { "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow" }, "sdPublisher": { "@id": "https://nf-co.re/" }, - "url": ["https://github.com/nf-core/variantbenchmarking", "https://nf-co.re/variantbenchmarking/dev/"], - "version": ["1.0dev"] + "url": [ + "https://github.com/nf-core/variantbenchmarking", + "https://nf-co.re/variantbenchmarking/dev/" + ], + "version": [ + "1.0dev" + ] }, { "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow", @@ -150,11 +170,11 @@ "version": "!>=24.04.2" }, { - "@id": "#d1132ae2-a4ec-465b-adcb-386595845ac2", + "@id": "#91c4f0d3-aae1-4ff3-b0fc-040d23c0493d", "@type": "TestSuite", "instance": [ { - "@id": "#5b1edf7d-b297-4c18-aff3-1adbc98ced26" + "@id": "#68e56bc1-38c8-4238-b744-2a682abe9825" } ], "mainEntity": { @@ -163,7 +183,7 @@ "name": "Test suite for nf-core/variantbenchmarking" }, { - "@id": "#5b1edf7d-b297-4c18-aff3-1adbc98ced26", + "@id": "#68e56bc1-38c8-4238-b744-2a682abe9825", "@type": "TestInstance", "name": "GitHub Actions workflow for testing nf-core/variantbenchmarking", "resource": "repos/nf-core/variantbenchmarking/actions/workflows/ci.yml", @@ -292,4 +312,4 @@ "url": "https://nf-co.re/" } ] -} +} \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf index af5e47b..d46043e 100644 --- a/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_variantbenchmarking_pipeline/main.nf @@ -117,7 +117,7 @@ workflow PIPELINE_COMPLETION { main: summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") def multiqc_reports = multiqc_report.toList() - + // // Completion email and summary // From ce8c7d72380d49f951f9ee535d8ee60e1accb336 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 13 Jan 2025 14:18:59 +0000 Subject: [PATCH 234/292] fix filtering --- conf/test.config | 2 +- conf/tests/germline_small.config | 2 +- conf/tests/somatic_snv.config | 2 +- conf/tests/somatic_sv.config | 2 +- subworkflows/local/vcf_variant_filtering.nf | 17 +++--- tests/germline_small.nf.test.snap | 66 +++++++++++---------- tests/germline_sv.nf.test.snap | 44 +++++++++----- tests/somatic_snv.nf.test.snap | 16 +++-- tests/somatic_sv.nf.test.snap | 35 ++++------- 9 files changed, 98 insertions(+), 88 deletions(-) diff --git a/conf/test.config b/conf/test.config index 5c34a7a..5c09764 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,7 +23,7 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "assets/samplesheet_small.csv" + input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_small.csv" outdir = "results" // Genome references diff --git a/conf/tests/germline_small.config b/conf/tests/germline_small.config index 514688c..871751a 100644 --- a/conf/tests/germline_small.config +++ b/conf/tests/germline_small.config @@ -34,7 +34,7 @@ params { variant_type = "small" method = 'happy,rtgtools' preprocess = "normalization,deduplication,prepy" - include_expression = 'FILTER="."' + include_expression = '(ILEN >= -5 && ILEN <= 5)' // truth information truth_id = "HG002" diff --git a/conf/tests/somatic_snv.config b/conf/tests/somatic_snv.config index ec4c8c6..83a92f2 100644 --- a/conf/tests/somatic_snv.config +++ b/conf/tests/somatic_snv.config @@ -33,6 +33,6 @@ params { truth_id = "SEQC2" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sSNV_truth_set_v1.0.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" } diff --git a/conf/tests/somatic_sv.config b/conf/tests/somatic_sv.config index 7fdfe7e..00e2151 100644 --- a/conf/tests/somatic_sv.config +++ b/conf/tests/somatic_sv.config @@ -32,6 +32,6 @@ params { truth_id = "SEQC2" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/13059_2022_2816_MOESM4_ESM.vcf.gz" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" } diff --git a/subworkflows/local/vcf_variant_filtering.nf b/subworkflows/local/vcf_variant_filtering.nf index 7f4271f..6dba51d 100644 --- a/subworkflows/local/vcf_variant_filtering.nf +++ b/subworkflows/local/vcf_variant_filtering.nf @@ -16,14 +16,7 @@ workflow VCF_VARIANT_FILTERING { versions = Channel.empty() - // unzip vcf file, required for survivor filter - TABIX_BGZIP( - vcf_ch.map{ meta, vcf, index -> tuple(meta, vcf)} - ) - versions = versions.mix(TABIX_BGZIP.out.versions.first()) - vcf_ch = TABIX_BGZIP.out.output - - if(params.exclude_expression != null & params.include_expression != null){ + if(params.exclude_expression != null | params.include_expression != null){ // filter vcf files using bcftools expressions BCFTOOLS_FILTER( @@ -32,6 +25,14 @@ workflow VCF_VARIANT_FILTERING { versions = versions.mix(BCFTOOLS_FILTER.out.versions.first()) vcf_ch = BCFTOOLS_FILTER.out.vcf } + else{ + // unzip vcf file, required for survivor filter + TABIX_BGZIP( + vcf_ch.map{ meta, vcf, index -> tuple(meta, vcf)} + ) + versions = versions.mix(TABIX_BGZIP.out.versions.first()) + vcf_ch = TABIX_BGZIP.out.output + } if(params.min_sv_size > 0 | params.max_sv_size != -1 | params.min_allele_freq != -1 | params.min_num_reads != -1){ diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 554f702..fabe72f 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -6,6 +6,9 @@ "BCFTOOLS_DEDUP": { "bcftools": 1.18 }, + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_MERGE": { "bcftools": 1.2 }, @@ -45,9 +48,6 @@ "RTGTOOLS_VCFEVAL": { "rtg-tools": "3.12.1" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, "TABIX_TABIX": { "tabix": "1.19.1" }, @@ -162,6 +162,7 @@ "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp.vcf.gz.tbi", "small/test1/benchmarks/rtgtools/test1.HG002.strelka.weighted_roc.tsv.gz", "small/test1/preprocess", + "small/test1/preprocess/HG002.strelka.variants.chr21.rh.norm.filter.vcf", "small/test1/preprocess/test1.HG002.strelka.prepy.vcf.gz", "small/test1/preprocess/test1.dedup.sort.vcf.gz", "small/test1/stats", @@ -196,6 +197,7 @@ "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp.vcf.gz.tbi", "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.weighted_roc.tsv.gz", "small/test2/preprocess", + "small/test2/preprocess/HG002.bcftools.chr21.rh.norm.filter.vcf", "small/test2/preprocess/test2.HG002.bcftools.prepy.vcf.gz", "small/test2/preprocess/test2.dedup.sort.vcf.gz", "small/test2/stats", @@ -228,10 +230,10 @@ ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T10:55:37.880792825" + "timestamp": "2025-01-13T13:40:17.22451061" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -240,6 +242,9 @@ "BCFTOOLS_DEDUP": { "bcftools": 1.18 }, + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_MERGE": { "bcftools": 1.2 }, @@ -279,9 +284,6 @@ "RTGTOOLS_VCFEVAL": { "rtg-tools": "3.12.1" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, "TABIX_TABIX": { "tabix": "1.19.1" }, @@ -491,6 +493,7 @@ "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp.vcf.gz.tbi", "small/test1/benchmarks/rtgtools/test1.HG002.strelka.weighted_roc.tsv.gz", "small/test1/preprocess", + "small/test1/preprocess/HG002.strelka.variants.chr21.rh.norm.filter.vcf", "small/test1/preprocess/test1.HG002.strelka.prepy.vcf.gz", "small/test1/preprocess/test1.dedup.sort.vcf.gz", "small/test1/stats", @@ -525,6 +528,7 @@ "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp.vcf.gz.tbi", "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.weighted_roc.tsv.gz", "small/test2/preprocess", + "small/test2/preprocess/HG002.bcftools.chr21.rh.norm.filter.vcf", "small/test2/preprocess/test2.HG002.bcftools.prepy.vcf.gz", "small/test2/preprocess/test2.dedup.sort.vcf.gz", "small/test2/stats", @@ -549,32 +553,32 @@ "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.bcftools_stats.txt:md5,b215fc0030c53bc8887e28b23b97efb6", - "test1.HG002.strelka.extended.csv:md5,098dc352d1beeab27e72f97e64fc182e", - "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,bd1f1a1e138f75511de7aae157fa2fa4", - "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8912022beb3c5b234d29300b764b56bc", - "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,63279f14076a5a9919a4d30b0fbb7746", - "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,77aedefc4516566b136acfba82bed147", - "test1.HG002.strelka.roc.all.csv.gz:md5,ccefcf1ff7ef04c9d97b8afbbcfb3c51", - "test1.HG002.strelka.summary.csv:md5,c14117d825b861657b38c058644896f9", - "test1.HG002.strelka.phasing.txt:md5,5ab9ffbd7c18a5512851086b6e7ecf59", - "test1.HG002.strelka.summary.txt:md5,e045d6a048bdec24cb55f9dd1b55ffe7", - "test1.strelka.bcftools_stats.txt:md5,f9f8cf5c1be9c88754888ef8cbfb06f4", - "test2.HG002.bcftools.extended.csv:md5,14392c3b5a1c060d63414cfc10e670b6", - "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,507b54e5b6d1f956f557b39a7dc38ff4", - "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,4694d6169ce0819d36768045a7ef43d0", - "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,83d1fa126072c16b0471fb8877c5c818", - "test2.HG002.bcftools.roc.Locations.SNP.csv.gz:md5,10045ce81f85c51e142a7ff30d7579bb", - "test2.HG002.bcftools.roc.all.csv.gz:md5,b176e3c9910ecf3209353682553c1a06", - "test2.HG002.bcftools.summary.csv:md5,3815ea18e8fc11f198a5821dee79a3c7", + "test1.HG002.strelka.extended.csv:md5,4362260b357ac0221414095f4c5a8981", + "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a5ba4044a89ae80fb0ddf95147b5ae4c", + "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,52edef7d20ac8a7e03771037f5c93fe4", + "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,f71e697d7ebaf1d670e5dc2c0e0106d3", + "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,c388d5a15ba2ae8dff709b030f1b4828", + "test1.HG002.strelka.roc.all.csv.gz:md5,59ebbe78bf428b5c2c78c8ff92f54545", + "test1.HG002.strelka.summary.csv:md5,60af846379cf4fe078fbed6b9d1e8178", + "test1.HG002.strelka.phasing.txt:md5,838e67ae5b9cd9e218095596c03fbee3", + "test1.HG002.strelka.summary.txt:md5,e79779d3faebe02bae943ddc17c4cf91", + "test1.strelka.bcftools_stats.txt:md5,492f42090004470e7e0ea7abc5f89bdf", + "test2.HG002.bcftools.extended.csv:md5,2f8ef20f46c821333ba970e3034a6ccd", + "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,cdf3fdb7c5b4c54d9896e37a99dbf4f9", + "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,9b16abcfe483356020c550e1292554ed", + "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,f71e697d7ebaf1d670e5dc2c0e0106d3", + "test2.HG002.bcftools.roc.Locations.SNP.csv.gz:md5,c388d5a15ba2ae8dff709b030f1b4828", + "test2.HG002.bcftools.roc.all.csv.gz:md5,e772fefec84f9a6e60c6979bac14cedc", + "test2.HG002.bcftools.summary.csv:md5,05722d23f523141fdce842a18f1d8aa2", "test2.HG002.bcftools.phasing.txt:md5,38920536b8c3e241e873c07ba61762e6", - "test2.HG002.bcftools.summary.txt:md5,b9a0c74a8b5af4f2c1c0623b61d7cbe3", - "test2.bcftools.bcftools_stats.txt:md5,57aff2f0a6f830e920869b987502a343" + "test2.HG002.bcftools.summary.txt:md5,a7bbcdf86cd3f1f7815ea9bc25b57b61", + "test2.bcftools.bcftools_stats.txt:md5,440fd66ee557b215bbacd05953215f7a" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T10:53:32.971378431" + "timestamp": "2025-01-13T14:00:31.553072869" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index 9af6025..84d6427 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -6,6 +6,9 @@ "BCFTOOLS_DEDUP": { "bcftools": 1.18 }, + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_NORM": { "bcftools": 1.18 }, @@ -264,6 +267,7 @@ "structural/test1/preprocess/manta.HG002.chr21.norm.vcf.gz", "structural/test1/preprocess/test1.dedup.sort.vcf.gz", "structural/test1/preprocess/test1.manta.svync.vcf.gz", + "structural/test1/preprocess/test1.norm.filter.filter.vcf", "structural/test1/preprocess/test1.norm.filter.vcf", "structural/test1/stats", "structural/test1/stats/bcftools", @@ -296,6 +300,7 @@ "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.sort.vcf.gz", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.vcf.gz", "structural/test2/preprocess/test2.dedup.sort.vcf.gz", + "structural/test2/preprocess/test2.norm.filter.filter.vcf", "structural/test2/preprocess/test2.norm.filter.vcf", "structural/test2/stats", "structural/test2/stats/bcftools", @@ -329,6 +334,7 @@ "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", "structural/test3/preprocess/test3.dedup.sort.vcf.gz", "structural/test3/preprocess/test3.dragen.svync.vcf.gz", + "structural/test3/preprocess/test3.norm.filter.filter.vcf", "structural/test3/preprocess/test3.norm.filter.vcf", "structural/test3/stats", "structural/test3/stats/bcftools", @@ -339,25 +345,25 @@ [ "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "test1.HG002.manta.distances:md5,33dcbe29f7129dc30c184b564803ec63", - "test1.HG002.manta.report:md5,91b0fbfd6fcdf45b85290f92c5a470fb", - "test1.manta.bcftools_stats.txt:md5,0e4cf1707b245c026fe6a0af6e8f8c0a", - "test1.manta_mqc.stats:md5,6f25a9372095052218110390e8e75c54", + "test1.HG002.manta.distances:md5,346f18a5cbeece98716951c8fc2aaea4", + "test1.HG002.manta.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", + "test1.manta.bcftools_stats.txt:md5,7d65792aa3a84de09675facf62135c93", + "test1.manta_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", "test2.HG002.merged.distances:md5,346f18a5cbeece98716951c8fc2aaea4", - "test2.HG002.merged.report:md5,6466d73155f88084efc69cad3628eb8c", - "test2.merged.bcftools_stats.txt:md5,576f9bc7e06bc2bbfccceb9da161a467", - "test2.merged_mqc.stats:md5,36d80468e13b583d0761b434d095312d", + "test2.HG002.merged.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", + "test2.merged.bcftools_stats.txt:md5,1445742129b0ee67d8706af3dcf0ab2d", + "test2.merged_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", "test3.HG002.dragen.distances:md5,346f18a5cbeece98716951c8fc2aaea4", - "test3.HG002.dragen.report:md5,b295d4867b7a96f0ca0bb5e8bb45eb68", - "test3.dragen.bcftools_stats.txt:md5,0683a5d3c47a5a6a43ad2b6a0387672e", - "test3.dragen_mqc.stats:md5,5c59cceb2e7d909d91074bb8e5804abc" + "test3.HG002.dragen.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", + "test3.dragen.bcftools_stats.txt:md5,5d2b48ac5f194f5a2cf01b9623a28cce", + "test3.dragen_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T10:42:14.227940401" + "timestamp": "2025-01-13T13:53:07.620717905" }, "-stub": { "content": [ @@ -366,6 +372,9 @@ "BCFTOOLS_DEDUP": { "bcftools": 1.18 }, + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_NORM": { "bcftools": 1.18 }, @@ -563,6 +572,7 @@ "structural/test1/preprocess/manta.HG002.chr21.norm.vcf.gz", "structural/test1/preprocess/test1.dedup.sort.vcf.gz", "structural/test1/preprocess/test1.manta.svync.vcf.gz", + "structural/test1/preprocess/test1.norm.filter.filter.vcf", "structural/test1/preprocess/test1.norm.filter.vcf", "structural/test1/stats", "structural/test1/stats/bcftools", @@ -595,6 +605,7 @@ "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.sort.vcf.gz", "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.vcf.gz", "structural/test2/preprocess/test2.dedup.sort.vcf.gz", + "structural/test2/preprocess/test2.norm.filter.filter.vcf", "structural/test2/preprocess/test2.norm.filter.vcf", "structural/test2/stats", "structural/test2/stats/bcftools", @@ -628,6 +639,7 @@ "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", "structural/test3/preprocess/test3.dedup.sort.vcf.gz", "structural/test3/preprocess/test3.dragen.svync.vcf.gz", + "structural/test3/preprocess/test3.norm.filter.filter.vcf", "structural/test3/preprocess/test3.norm.filter.vcf", "structural/test3/stats", "structural/test3/stats/bcftools", @@ -653,9 +665,9 @@ ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T10:44:36.4725572" + "timestamp": "2025-01-13T13:55:36.985456265" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 5f03580..45b7e8c 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -3,6 +3,9 @@ "content": [ 38, { + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_REHEADER": { "bcftools": 1.18 }, @@ -33,9 +36,6 @@ "PLOTS": { "r-base": "4.3.1" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, "TABIX_BGZIPTABIX": { "tabix": "1.19.1" }, @@ -90,6 +90,8 @@ "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.features.csv", "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.metrics.json", "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.stats.csv", + "snv/test1/preprocess", + "snv/test1/preprocess/test1.filter.vcf", "snv/test1/stats", "snv/test1/stats/bcftools", "snv/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", @@ -101,6 +103,7 @@ "snv/test2/benchmarks/sompy/test2.SEQC2.manta.stats.csv", "snv/test2/preprocess", "snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", + "snv/test2/preprocess/test2.filter.vcf", "snv/test2/stats", "snv/test2/stats/bcftools", "snv/test2/stats/bcftools/test2.manta.bcftools_stats.txt", @@ -112,6 +115,7 @@ "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.stats.csv", "snv/test3/preprocess", "snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", + "snv/test3/preprocess/test3.filter.vcf", "snv/test3/stats", "snv/test3/stats/bcftools", "snv/test3/stats/bcftools/test3.strelka.bcftools_stats.txt" @@ -124,9 +128,9 @@ ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T13:54:36.607079161" + "timestamp": "2025-01-13T14:15:33.922876808" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index b2ccd3d..f5cf4b6 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -3,6 +3,9 @@ "content": [ 69, { + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_REHEADER": { "bcftools": 1.18 }, @@ -71,10 +74,6 @@ "structural/multiqc", "structural/multiqc/multiqc_data", "structural/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", - "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", - "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", - "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", - "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", "structural/multiqc/multiqc_data/multiqc.log", "structural/multiqc/multiqc_data/multiqc_bcftools_stats.txt", "structural/multiqc/multiqc_data/multiqc_citations.txt", @@ -86,28 +85,16 @@ "structural/multiqc/multiqc_plots", "structural/multiqc/multiqc_plots/pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", - "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", - "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", - "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", - "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", "structural/multiqc/multiqc_plots/pdf/general_stats_table.pdf", "structural/multiqc/multiqc_plots/pdf/survivor-cnt.pdf", "structural/multiqc/multiqc_plots/pdf/survivor-pct.pdf", "structural/multiqc/multiqc_plots/png", "structural/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", - "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", - "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", - "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", - "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", "structural/multiqc/multiqc_plots/png/general_stats_table.png", "structural/multiqc/multiqc_plots/png/survivor-cnt.png", "structural/multiqc/multiqc_plots/png/survivor-pct.png", "structural/multiqc/multiqc_plots/svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", - "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", - "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", - "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", - "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", "structural/multiqc/multiqc_plots/svg/general_stats_table.svg", "structural/multiqc/multiqc_plots/svg/survivor-cnt.svg", "structural/multiqc/multiqc_plots/svg/survivor-pct.svg", @@ -166,6 +153,7 @@ "structural/test1/benchmarks/truvari/test1.SEQC2.tiddit.tp-comp.vcf.gz.tbi", "structural/test1/preprocess", "structural/test1/preprocess/HCC1395T_vs_HCC1395N.tiddit_sv_merge.sort.vcf.gz", + "structural/test1/preprocess/test1.filter.filter.vcf", "structural/test1/preprocess/test1.filter.vcf", "structural/test1/stats", "structural/test1/stats/bcftools", @@ -186,6 +174,7 @@ "structural/test2/benchmarks/truvari/test2.SEQC2.manta.tp-comp.vcf.gz.tbi", "structural/test2/preprocess", "structural/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", + "structural/test2/preprocess/test2.filter.filter.vcf", "structural/test2/preprocess/test2.filter.vcf", "structural/test2/stats", "structural/test2/stats/bcftools", @@ -196,16 +185,16 @@ [ "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", "SEQC2_mqc.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", - "test1.tiddit.bcftools_stats.txt:md5,56b036b7bd2d7368620713613dd0cae2", - "test1.tiddit_mqc.stats:md5,0eb749429a6072f6bf63d377e37514d1", - "test2.manta.bcftools_stats.txt:md5,1c4cc5ab438537308e7f08a6c202118c", - "test2.manta_mqc.stats:md5,73bc60cd2754202ddca8be6552e85ffa" + "test1.tiddit.bcftools_stats.txt:md5,aace5a23af8b02f56bcaf03722f9bb50", + "test1.tiddit_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", + "test2.manta.bcftools_stats.txt:md5,1e700076bd303ee079b23658485eb7d4", + "test2.manta_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T11:06:49.710866299" + "timestamp": "2025-01-13T14:18:00.311055864" } } \ No newline at end of file From 7a84387616c772fff28eea33907a4c2f604afbdd Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 13 Jan 2025 14:49:11 +0000 Subject: [PATCH 235/292] renew snapshots --- tests/liftover_test.nf.test.snap | 19 ++++++++++------- tests/liftover_truth.nf.test.snap | 34 ++++++++++++++++--------------- tests/somatic_indel.nf.test.snap | 17 +++++++++------- 3 files changed, 40 insertions(+), 30 deletions(-) diff --git a/tests/liftover_test.nf.test.snap b/tests/liftover_test.nf.test.snap index 7dfaaf1..023d23d 100644 --- a/tests/liftover_test.nf.test.snap +++ b/tests/liftover_test.nf.test.snap @@ -3,6 +3,9 @@ "content": [ 67, { + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_REHEADER": { "bcftools": 1.18 }, @@ -164,6 +167,7 @@ "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz", "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz.tbi", "structural/test1/preprocess", + "structural/test1/preprocess/test1.filter.filter.vcf", "structural/test1/preprocess/test1.filter.vcf", "structural/test1/stats", "structural/test1/stats/bcftools", @@ -183,6 +187,7 @@ "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz", "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz.tbi", "structural/test2/preprocess", + "structural/test2/preprocess/test2.filter.filter.vcf", "structural/test2/preprocess/test2.filter.vcf", "structural/test2/stats", "structural/test2/stats/bcftools", @@ -193,16 +198,16 @@ [ "HG002.bcftools_stats.txt:md5,7c007a87b5730787e570712e784d3cc3", "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "test1.delly.bcftools_stats.txt:md5,d32a9bea755d2065e19e88b9159e2502", - "test1.delly_mqc.stats:md5,e140ad55975c767578b0dd6aff58ba29", - "test2.manta.bcftools_stats.txt:md5,99f8a0b12efa30c6253b44ebfeb9b1d6", - "test2.manta_mqc.stats:md5,1d74b41c6b970992e3a39682b0a68e23" + "test1.delly.bcftools_stats.txt:md5,aace5a23af8b02f56bcaf03722f9bb50", + "test1.delly_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", + "test2.manta.bcftools_stats.txt:md5,1e700076bd303ee079b23658485eb7d4", + "test2.manta_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-03T15:52:10.533611573" + "timestamp": "2025-01-13T14:41:30.39094733" } } \ No newline at end of file diff --git a/tests/liftover_truth.nf.test.snap b/tests/liftover_truth.nf.test.snap index ef7a85a..b6d9288 100644 --- a/tests/liftover_truth.nf.test.snap +++ b/tests/liftover_truth.nf.test.snap @@ -6,6 +6,9 @@ "BCFTOOLS_DEDUP": { "bcftools": 1.18 }, + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_MERGE": { "bcftools": 1.2 }, @@ -45,9 +48,6 @@ "RTGTOOLS_VCFEVAL": { "rtg-tools": "3.12.1" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, "TABIX_BGZIPTABIX": { "tabix": "1.19.1" }, @@ -269,6 +269,7 @@ "small/test1/benchmarks/rtgtools/test1.HG002.strelka.weighted_roc.tsv.gz", "small/test1/preprocess", "small/test1/preprocess/test1.dedup.sort.vcf.gz", + "small/test1/preprocess/test1.norm.filter.vcf", "small/test1/stats", "small/test1/stats/bcftools", "small/test1/stats/bcftools/test1.strelka.bcftools_stats.txt", @@ -302,6 +303,7 @@ "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.weighted_roc.tsv.gz", "small/test2/preprocess", "small/test2/preprocess/test2.dedup.sort.vcf.gz", + "small/test2/preprocess/test2.norm.filter.vcf", "small/test2/stats", "small/test2/stats/bcftools", "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" @@ -325,16 +327,16 @@ "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", - "test1.HG002.strelka.extended.csv:md5,fadf06276179c1cc388007e5651fb9af", - "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a08665be8ea20855a14d6418d07521dc", - "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8d4a20a5914379230952aeb252845e2d", - "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,c546609a3e23b4c5c97ee0fc2e864e07", - "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,8d2600c17665ed1299e2dd483705ddaa", - "test1.HG002.strelka.roc.all.csv.gz:md5,d0a4408438b2182cb82caec918c090f1", - "test1.HG002.strelka.summary.csv:md5,001fa2371777d1d2dc3eb0f4dcaca197", - "test1.HG002.strelka.phasing.txt:md5,0c9340e64e032b5f74d0ea3773464afd", - "test1.HG002.strelka.summary.txt:md5,4b775edb878759727ef66d5d5b2b9ee8", - "test1.strelka.bcftools_stats.txt:md5,f9f8cf5c1be9c88754888ef8cbfb06f4", + "test1.HG002.strelka.extended.csv:md5,111a236ca6b747face70e8111c09c51f", + "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,5425b34f4688dda4d55f9f7a73667b18", + "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,eb9834ec5660d1e32ade0c856b9cfb7c", + "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,778eb91f54cb830fb91fcddc688668e0", + "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,516a4b58814cf32245a5b2f2c0445c8e", + "test1.HG002.strelka.roc.all.csv.gz:md5,a69fb6ae5cd051d4607da0224009f1d1", + "test1.HG002.strelka.summary.csv:md5,d29cba645e161dbdeb6247f57fce5780", + "test1.HG002.strelka.phasing.txt:md5,38920536b8c3e241e873c07ba61762e6", + "test1.HG002.strelka.summary.txt:md5,6c43a0d1ad065f851942828259d551a5", + "test1.strelka.bcftools_stats.txt:md5,7d65792aa3a84de09675facf62135c93", "test2.HG002.bcftools.extended.csv:md5,05c6efd85a9b9823a5ddfe6619be3323", "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,463cee548b7bdf133c0ae9b5803a50ca", "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,424e39c327ae6ef9001942bc895abdc9", @@ -348,9 +350,9 @@ ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T10:20:35.930923969" + "timestamp": "2025-01-13T14:48:00.444469734" } } \ No newline at end of file diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 1f04021..5f59ce6 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -3,6 +3,9 @@ "content": [ 27, { + "BCFTOOLS_FILTER": { + "bcftools": 1.2 + }, "BCFTOOLS_REHEADER": { "bcftools": 1.18 }, @@ -33,9 +36,6 @@ "PLOTS": { "r-base": "4.3.1" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, "TABIX_BGZIPTABIX": { "tabix": "1.19.1" }, @@ -150,6 +150,8 @@ "indel/test1/benchmarks/sompy/test1.SEQC2.freebayes.features.csv", "indel/test1/benchmarks/sompy/test1.SEQC2.freebayes.metrics.json", "indel/test1/benchmarks/sompy/test1.SEQC2.freebayes.stats.csv", + "indel/test1/preprocess", + "indel/test1/preprocess/test1.filter.vcf", "indel/test1/stats", "indel/test1/stats/bcftools", "indel/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", @@ -161,6 +163,7 @@ "indel/test2/benchmarks/sompy/test2.SEQC2.strelka.stats.csv", "indel/test2/preprocess", "indel/test2/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_indels.sort.vcf.gz", + "indel/test2/preprocess/test2.filter.vcf", "indel/test2/stats", "indel/test2/stats/bcftools", "indel/test2/stats/bcftools/test2.strelka.bcftools_stats.txt", @@ -170,13 +173,13 @@ [ "SEQC2.bcftools_stats.txt:md5,e530daf4f6a4923f1cd85c51893d5747", "test1.freebayes.bcftools_stats.txt:md5,c5025b5c6256c1d808badee73a7c220c", - "test2.strelka.bcftools_stats.txt:md5,ec553881bc70c786e8cec95192823634" + "test2.strelka.bcftools_stats.txt:md5,1e700076bd303ee079b23658485eb7d4" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.10.2" + "nf-test": "0.9.2", + "nextflow": "24.10.3" }, - "timestamp": "2024-12-10T11:27:48.141030523" + "timestamp": "2025-01-13T14:32:00.898226798" } } \ No newline at end of file From eb37c8200f539b8b8d352d26658c9da017f21678 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 13 Jan 2025 16:35:57 +0100 Subject: [PATCH 236/292] Update subworkflows/local/vcf_variant_filtering.nf Co-authored-by: Nicolas Vannieuwkerke <101190534+nvnieuwk@users.noreply.github.com> --- subworkflows/local/vcf_variant_filtering.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/vcf_variant_filtering.nf b/subworkflows/local/vcf_variant_filtering.nf index 6dba51d..5cba729 100644 --- a/subworkflows/local/vcf_variant_filtering.nf +++ b/subworkflows/local/vcf_variant_filtering.nf @@ -16,7 +16,7 @@ workflow VCF_VARIANT_FILTERING { versions = Channel.empty() - if(params.exclude_expression != null | params.include_expression != null){ + if(params.exclude_expression != null || params.include_expression != null){ // filter vcf files using bcftools expressions BCFTOOLS_FILTER( From d9baa5b501ba3cc1ff420d52a097180f866d5068 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 14 Jan 2025 15:53:08 +0000 Subject: [PATCH 237/292] update in bcftools view and norm, fixing naming complications --- conf/modules.config | 15 +- conf/test.config | 2 +- modules.json | 4 +- modules/nf-core/bcftools/norm/environment.yml | 4 +- modules/nf-core/bcftools/norm/main.nf | 22 +- modules/nf-core/bcftools/norm/meta.yml | 96 ++- .../nf-core/bcftools/norm/tests/main.nf.test | 563 +++++++++++++ .../bcftools/norm/tests/main.nf.test.snap | 758 ++++++++++++++++++ .../bcftools/norm/tests/nextflow.bcf.config | 4 + .../norm/tests/nextflow.bcf_gz.config | 4 + .../bcftools/norm/tests/nextflow.config | 4 + .../bcftools/norm/tests/nextflow.vcf.config | 4 + .../norm/tests/nextflow.vcf_gz.config | 4 + modules/nf-core/bcftools/norm/tests/tags.yml | 2 + .../bcftools/norm/tests/vcf_gz_index.config | 4 + .../norm/tests/vcf_gz_index_csi.config | 4 + .../norm/tests/vcf_gz_index_tbi.config | 4 + modules/nf-core/bcftools/view/environment.yml | 4 +- modules/nf-core/bcftools/view/main.nf | 16 +- modules/nf-core/bcftools/view/meta.yml | 108 ++- .../nf-core/bcftools/view/tests/main.nf.test | 217 ++++- .../bcftools/view/tests/main.nf.test.snap | 300 ++++++- .../bcftools/view/tests/vcf_gz_index.config | 4 + .../view/tests/vcf_gz_index_csi.config | 4 + .../view/tests/vcf_gz_index_tbi.config | 4 + nextflow_schema.json | 8 +- subworkflows/local/prepare_vcfs_test.nf | 51 +- subworkflows/local/prepare_vcfs_truth.nf | 36 +- 28 files changed, 2093 insertions(+), 157 deletions(-) create mode 100644 modules/nf-core/bcftools/norm/tests/main.nf.test create mode 100644 modules/nf-core/bcftools/norm/tests/main.nf.test.snap create mode 100644 modules/nf-core/bcftools/norm/tests/nextflow.bcf.config create mode 100644 modules/nf-core/bcftools/norm/tests/nextflow.bcf_gz.config create mode 100644 modules/nf-core/bcftools/norm/tests/nextflow.config create mode 100644 modules/nf-core/bcftools/norm/tests/nextflow.vcf.config create mode 100644 modules/nf-core/bcftools/norm/tests/nextflow.vcf_gz.config create mode 100644 modules/nf-core/bcftools/norm/tests/tags.yml create mode 100644 modules/nf-core/bcftools/norm/tests/vcf_gz_index.config create mode 100644 modules/nf-core/bcftools/norm/tests/vcf_gz_index_csi.config create mode 100644 modules/nf-core/bcftools/norm/tests/vcf_gz_index_tbi.config create mode 100644 modules/nf-core/bcftools/view/tests/vcf_gz_index.config create mode 100644 modules/nf-core/bcftools/view/tests/vcf_gz_index_csi.config create mode 100644 modules/nf-core/bcftools/view/tests/vcf_gz_index_tbi.config diff --git a/conf/modules.config b/conf/modules.config index ea28690..9e132a7 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -26,10 +26,17 @@ process { ] } // standardization and normalization tools + withName: "BCFTOOLS_NORM" { ext.prefix = { vcf.baseName - ".vcf" + ".norm"} - ext.args = {"--output-type z -m-any -c w" } + ext.args = {"--output-type z -c w --write-index=tbi" } + } + + withName: "BCFTOOLS_SPLIT_MULTI" { + ext.prefix = { vcf.baseName - ".vcf" + ".split"} + ext.args = {"--output-type z -m-any -c w --write-index=tbi" } } + withName: "VARIANT_EXTRACTOR" { ext.prefix = { input.baseName - ".vcf" } publishDir = [ @@ -123,9 +130,9 @@ process { ] } withName: BCFTOOLS_VIEW_CONTIGS { - ext.prefix = { vcf.baseName - ".vcf" + ".nocontigs" } + ext.prefix = { vcf.baseName - ".vcf.gz" + ".nocontigs" } ext.args = {[ - "--output-type v", + "--output-type z --write-index=tbi", (params.genome.contains("38"))? "-r chr1,chr2,chr3,chr4,chr5,chr6,chr7,chr8,chr9,chr10,chr11,chr12,chr13,chr14,chr15,chr16,chr17,chr18,chr19,chr20,chr21,chr22,chrX,chrY" : "-r 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,X,Y" ].join(' ').trim() } publishDir = [ @@ -346,7 +353,7 @@ process { // Don't publish results for these processes // process { - withName: 'TABIX_TABIX|TABIX_BGZIP|TABIX_BGZIPTABIX|BGZIP_TABIX|SURVIVOR_MERGE|BCFTOOLS_MERGE|REFORMAT_HEADER|BCFTOOLS_NORM|BCFTOOLS_DEDUP|BCFTOOLS_REHEADER|SORT_BED|UCSC_LIFTOVER|PICARD_LIFTOVERVCF|BCFTOOLS_VIEW_SUBSAMPLE|CREATE_DATAVZRD_INPUT' { + withName: 'TABIX_TABIX|TABIX_BGZIP|TABIX_BGZIPTABIX|BGZIP_TABIX|SURVIVOR_MERGE|BCFTOOLS_MERGE|REFORMAT_HEADER|BCFTOOLS_SPLIT_MULTI|BCFTOOLS_DEDUP|BCFTOOLS_REHEADER|SORT_BED|UCSC_LIFTOVER|PICARD_LIFTOVERVCF|BCFTOOLS_VIEW_SUBSAMPLE|CREATE_DATAVZRD_INPUT' { publishDir = [ path: { "${params.outdir}/test" }, enabled: false diff --git a/conf/test.config b/conf/test.config index 5c09764..871b85d 100644 --- a/conf/test.config +++ b/conf/test.config @@ -33,7 +33,7 @@ params { analysis = 'germline' variant_type = "small" method = 'happy,rtgtools' - preprocess = "normalization,deduplication,prepy" + preprocess = "normalize,deduplicate,prepy,split_multiallelic,filter_contigs" include_expression = 'FILTER="."' // truth information diff --git a/modules.json b/modules.json index e43cf69..1020492 100644 --- a/modules.json +++ b/modules.json @@ -22,7 +22,7 @@ }, "bcftools/norm": { "branch": "master", - "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "bcftools/query": { @@ -47,7 +47,7 @@ }, "bcftools/view": { "branch": "master", - "git_sha": "1013101da4252623fd7acf19cc581bae91d4f839", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "bedtools/merge": { diff --git a/modules/nf-core/bcftools/norm/environment.yml b/modules/nf-core/bcftools/norm/environment.yml index fe80e4e..5c00b11 100644 --- a/modules/nf-core/bcftools/norm/environment.yml +++ b/modules/nf-core/bcftools/norm/environment.yml @@ -1,7 +1,5 @@ -name: bcftools_norm channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::bcftools=1.18 + - bioconda::bcftools=1.20 diff --git a/modules/nf-core/bcftools/norm/main.nf b/modules/nf-core/bcftools/norm/main.nf index 47d3dab..bd7a250 100644 --- a/modules/nf-core/bcftools/norm/main.nf +++ b/modules/nf-core/bcftools/norm/main.nf @@ -4,16 +4,18 @@ process BCFTOOLS_NORM { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'biocontainers/bcftools:1.18--h8b25389_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.20--h8b25389_0': + 'biocontainers/bcftools:1.20--h8b25389_0' }" input: tuple val(meta), path(vcf), path(tbi) tuple val(meta2), path(fasta) output: - tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + tuple val(meta), path("*.tbi") , emit: tbi, optional: true + tuple val(meta), path("*.csi") , emit: csi, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -30,7 +32,7 @@ process BCFTOOLS_NORM { """ bcftools norm \\ --fasta-ref ${fasta} \\ - --output ${prefix}.${extension}\\ + --output ${prefix}.${extension} \\ $args \\ --threads $task.cpus \\ ${vcf} @@ -49,8 +51,16 @@ process BCFTOOLS_NORM { args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf.gz" + def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" : + args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" : + args.contains("--write-index") || args.contains("-W") ? "csi" : + "" + def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch" + def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : "" + """ - touch ${prefix}.${extension} + ${create_cmd} ${prefix}.${extension} + ${create_index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bcftools/norm/meta.yml b/modules/nf-core/bcftools/norm/meta.yml index 1f3e1b6..b6edeb4 100644 --- a/modules/nf-core/bcftools/norm/meta.yml +++ b/modules/nf-core/bcftools/norm/meta.yml @@ -13,46 +13,70 @@ tools: documentation: http://www.htslib.org/doc/bcftools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] + identifier: biotools:bcftools input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: | - The vcf file to be normalized - e.g. 'file1.vcf' - pattern: "*.{vcf,vcf.gz}" - - tbi: - type: file - description: | - An optional index of the VCF file (for when the VCF is compressed) - pattern: "*.vcf.gz.tbi" - - meta2: - type: map - description: | - Groovy Map containing reference information - e.g. [ id:'genome' ] - - fasta: - type: file - description: FASTA reference file - pattern: "*.{fasta,fa}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + The vcf file to be normalized + e.g. 'file1.vcf' + pattern: "*.{vcf,vcf.gz}" + - tbi: + type: file + description: | + An optional index of the VCF file (for when the VCF is compressed) + pattern: "*.vcf.gz.tbi" + - - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] + - fasta: + type: file + description: FASTA reference file + pattern: "*.{fasta,fa}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - vcf: - type: file - description: One of uncompressed VCF (.vcf), compressed VCF (.vcf.gz), compressed BCF (.bcf.gz) or uncompressed BCF (.bcf) normalized output file - pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.{vcf,vcf.gz,bcf,bcf.gz}": + type: file + description: One of uncompressed VCF (.vcf), compressed VCF (.vcf.gz), compressed + BCF (.bcf.gz) or uncompressed BCF (.bcf) normalized output file + pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" + - tbi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.tbi": + type: file + description: Alternative VCF file index + pattern: "*.tbi" + - csi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.csi": + type: file + description: Default VCF file index + pattern: "*.csi" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@abhi18av" - "@ramprasadn" diff --git a/modules/nf-core/bcftools/norm/tests/main.nf.test b/modules/nf-core/bcftools/norm/tests/main.nf.test new file mode 100644 index 0000000..dbc4150 --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/main.nf.test @@ -0,0 +1,563 @@ +nextflow_process { + + name "Test Process BCFTOOLS_NORM" + script "../main.nf" + process "BCFTOOLS_NORM" + + tag "modules" + tag "modules_nfcore" + tag "bcftools" + tag "bcftools/norm" + + test("sarscov2 - [ vcf, [] ], fasta") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, [] ], fasta - vcf_gz_index") { + + config "./vcf_gz_index.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } } + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [ vcf, [] ], fasta - vcf_gz_index_csi") { + + config "./vcf_gz_index_csi.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } } + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [ vcf, [] ], fasta - vcf_gz_index_tbi") { + + config "./vcf_gz_index_tbi.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } } + ).match() }, + { assert process.out.tbi[0][1].endsWith(".tbi") } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta") { + + config "./nextflow.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - vcf output") { + + config "./nextflow.vcf.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - vcf_gz output") { + + config "./nextflow.vcf.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - bcf output") { + + config "./nextflow.bcf.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - bcf_gz output") { + + config "./nextflow.bcf_gz.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, [] ], fasta - stub") { + + config "./nextflow.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta -stub") { + + config "./nextflow.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - vcf output -stub") { + + config "./nextflow.vcf.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - vcf_gz output - stub") { + + config "./nextflow.vcf.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - bcf output - stub") { + + config "./nextflow.bcf.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, tbi ], fasta - bcf_gz output - stub") { + + config "./nextflow.bcf_gz.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [ vcf, [] ], fasta - vcf_gz_index - stub") { + + config "./vcf_gz_index.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [ vcf, [] ], fasta - vcf_gz_index_csi - stub") { + + config "./vcf_gz_index_csi.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.gz', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - 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[ vcf, [] ], fasta - vcf_gz_index_csi": { + "content": [ + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,63e5adbaf3dd94550e9e3d7935dd28db" + ] + ], + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.csi" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T13:53:09.808834237" + }, + "sarscov2 - [ vcf, tbi ], fasta - bcf output": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test_norm.bcf.gz:md5,638c3c25bdd495c90ecbccb69ee77f07" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,ff760495922469e56d0fc3372773000d" + ], + "csi": [ + + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "test" + }, + "test_norm.bcf.gz:md5,638c3c25bdd495c90ecbccb69ee77f07" + ] + ], + "versions": [ + "versions.yml:md5,ff760495922469e56d0fc3372773000d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-04T14:37:42.141945244" + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/norm/tests/nextflow.bcf.config b/modules/nf-core/bcftools/norm/tests/nextflow.bcf.config new file mode 100644 index 0000000..b79af86 --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/nextflow.bcf.config @@ -0,0 +1,4 @@ +process { + ext.args = '-m -any --output-type b --no-version' + ext.prefix = "test_norm" +} diff --git a/modules/nf-core/bcftools/norm/tests/nextflow.bcf_gz.config b/modules/nf-core/bcftools/norm/tests/nextflow.bcf_gz.config new file mode 100644 index 0000000..f36f397 --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/nextflow.bcf_gz.config @@ -0,0 +1,4 @@ +process { + ext.args = '-m -any --output-type u --no-version' + ext.prefix = "test_norm" +} diff --git a/modules/nf-core/bcftools/norm/tests/nextflow.config b/modules/nf-core/bcftools/norm/tests/nextflow.config new file mode 100644 index 0000000..510803b --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/nextflow.config @@ -0,0 +1,4 @@ +process { + ext.args = '-m -any --no-version' + ext.prefix = "test_norm" +} diff --git a/modules/nf-core/bcftools/norm/tests/nextflow.vcf.config b/modules/nf-core/bcftools/norm/tests/nextflow.vcf.config new file mode 100644 index 0000000..10bf93e --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/nextflow.vcf.config @@ -0,0 +1,4 @@ +process { + ext.args = '-m -any --output-type v --no-version' + ext.prefix = "test_norm" +} diff --git a/modules/nf-core/bcftools/norm/tests/nextflow.vcf_gz.config b/modules/nf-core/bcftools/norm/tests/nextflow.vcf_gz.config new file mode 100644 index 0000000..b31dd2d --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/nextflow.vcf_gz.config @@ -0,0 +1,4 @@ +process { + ext.args = '-m -any --output-type z ---no-version' + ext.prefix = "test_norm" +} diff --git a/modules/nf-core/bcftools/norm/tests/tags.yml b/modules/nf-core/bcftools/norm/tests/tags.yml new file mode 100644 index 0000000..f6f5e35 --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/tags.yml @@ -0,0 +1,2 @@ +bcftools/norm: + - "modules/nf-core/bcftools/norm/**" diff --git a/modules/nf-core/bcftools/norm/tests/vcf_gz_index.config b/modules/nf-core/bcftools/norm/tests/vcf_gz_index.config new file mode 100644 index 0000000..7dd696e --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/vcf_gz_index.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index --no-version" +} diff --git a/modules/nf-core/bcftools/norm/tests/vcf_gz_index_csi.config b/modules/nf-core/bcftools/norm/tests/vcf_gz_index_csi.config new file mode 100644 index 0000000..aebffb6 --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/vcf_gz_index_csi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=csi --no-version" +} diff --git a/modules/nf-core/bcftools/norm/tests/vcf_gz_index_tbi.config b/modules/nf-core/bcftools/norm/tests/vcf_gz_index_tbi.config new file mode 100644 index 0000000..b192ae7 --- /dev/null +++ b/modules/nf-core/bcftools/norm/tests/vcf_gz_index_tbi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=tbi --no-version" +} diff --git a/modules/nf-core/bcftools/view/environment.yml b/modules/nf-core/bcftools/view/environment.yml index 8937c6d..5c00b11 100644 --- a/modules/nf-core/bcftools/view/environment.yml +++ b/modules/nf-core/bcftools/view/environment.yml @@ -1,7 +1,5 @@ -name: bcftools_view channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::bcftools=1.18 + - bioconda::bcftools=1.20 diff --git a/modules/nf-core/bcftools/view/main.nf b/modules/nf-core/bcftools/view/main.nf index 5237adc..7fe4303 100644 --- a/modules/nf-core/bcftools/view/main.nf +++ b/modules/nf-core/bcftools/view/main.nf @@ -4,8 +4,8 @@ process BCFTOOLS_VIEW { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'biocontainers/bcftools:1.18--h8b25389_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.20--h8b25389_0': + 'biocontainers/bcftools:1.20--h8b25389_0' }" input: tuple val(meta), path(vcf), path(index) @@ -15,6 +15,8 @@ process BCFTOOLS_VIEW { output: tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + tuple val(meta), path("*.tbi") , emit: tbi, optional: true + tuple val(meta), path("*.csi") , emit: csi, optional: true path "versions.yml" , emit: versions when: @@ -55,8 +57,16 @@ process BCFTOOLS_VIEW { args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf" + def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" : + args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" : + args.contains("--write-index") || args.contains("-W") ? "csi" : + "" + def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch" + def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : "" + """ - touch ${prefix}.${extension} + ${create_cmd} ${prefix}.${extension} + ${create_index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bcftools/view/meta.yml b/modules/nf-core/bcftools/view/meta.yml index 6baa34a..aa7785f 100644 --- a/modules/nf-core/bcftools/view/meta.yml +++ b/modules/nf-core/bcftools/view/meta.yml @@ -1,5 +1,6 @@ name: bcftools_view -description: View, subset and filter VCF or BCF files by position and filtering expression. Convert between VCF and BCF +description: View, subset and filter VCF or BCF files by position and filtering expression. + Convert between VCF and BCF keywords: - variant calling - view @@ -13,51 +14,74 @@ tools: documentation: http://www.htslib.org/doc/bcftools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] + identifier: biotools:bcftools input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: | - The vcf file to be inspected. - e.g. 'file.vcf' - - index: - type: file - description: | - The tab index for the VCF file to be inspected. - e.g. 'file.tbi' - - regions: - type: file - description: | - Optionally, restrict the operation to regions listed in this file. - e.g. 'file.vcf' - - targets: - type: file - description: | - Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files) - e.g. 'file.vcf' - - samples: - type: file - description: | - Optional, file of sample names to be included or excluded. - e.g. 'file.tsv' + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: | + The vcf file to be inspected. + e.g. 'file.vcf' + - index: + type: file + description: | + The tab index for the VCF file to be inspected. + e.g. 'file.tbi' + - - regions: + type: file + description: | + Optionally, restrict the operation to regions listed in this file. + e.g. 'file.vcf' + - - targets: + type: file + description: | + Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files) + e.g. 'file.vcf' + - - samples: + type: file + description: | + Optional, file of sample names to be included or excluded. + e.g. 'file.tsv' output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - vcf: - type: file - description: VCF normalized output file - pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.{vcf,vcf.gz,bcf,bcf.gz}": + type: file + description: VCF normalized output file + pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" + - tbi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.tbi": + type: file + description: Alternative VCF file index + pattern: "*.tbi" + - csi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.csi": + type: file + description: Default VCF file index + pattern: "*.csi" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@abhi18av" maintainers: diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test b/modules/nf-core/bcftools/view/tests/main.nf.test index c285674..1e60c50 100644 --- a/modules/nf-core/bcftools/view/tests/main.nf.test +++ b/modules/nf-core/bcftools/view/tests/main.nf.test @@ -9,17 +9,17 @@ nextflow_process { tag "bcftools" tag "bcftools/view" - config "./nextflow.config" - test("sarscov2 - [vcf, tbi], [], [], []") { + config "./nextflow.config" + when { process { """ input[0] = [ [ id:'out', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) ] input[1] = [] input[2] = [] @@ -40,18 +40,122 @@ nextflow_process { } + test("sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index") { + + config "./vcf_gz_index.config" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_csi") { + + config "./vcf_gz_index_csi.config" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_tbi") { + + config "./vcf_gz_index_tbi.config" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.tbi[0][1].endsWith(".tbi") } + ) + } + + } + test("sarscov2 - [vcf, tbi], vcf, tsv, []") { + config "./nextflow.config" + when { process { """ input[0] = [ [ id:'out', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) ] - input[1] = file(params.test_data['sarscov2']['illumina']['test3_vcf_gz'], checkIfExists: true) - input[2] = file(params.test_data['sarscov2']['illumina']['test2_vcf_targets_tsv_gz'], checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test3.vcf.gz', checkIfExists: true) + input[2] = file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test2.targets.tsv.gz', checkIfExists: true) input[3] = [] """ } @@ -71,6 +175,7 @@ nextflow_process { test("sarscov2 - [vcf, tbi], [], [], [] - stub") { + config "./nextflow.config" options "-stub" when { @@ -78,8 +183,8 @@ nextflow_process { """ input[0] = [ [ id:'out', single_end:false ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) ] input[1] = [] input[2] = [] @@ -100,4 +205,94 @@ nextflow_process { } -} + test("sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index - stub") { + + config "./vcf_gz_index.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_csi - stub") { + + config "./vcf_gz_index_csi.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_tbi - stub") { + + config "./vcf_gz_index_tbi.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'out', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz.tbi', checkIfExists: true) + ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.tbi[0][1].endsWith(".tbi") } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/main.nf.test.snap b/modules/nf-core/bcftools/view/tests/main.nf.test.snap index b59be93..fec22e3 100644 --- a/modules/nf-core/bcftools/view/tests/main.nf.test.snap +++ b/modules/nf-core/bcftools/view/tests/main.nf.test.snap @@ -1,4 +1,214 @@ { + "sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_csi - stub": { + "content": [ + { + "0": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ], + "csi": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:14:38.717458272" + }, + "sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_tbi": { + "content": [ + [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + + ], + [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.tbi" + ] + ], + [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:13:44.760671384" + }, + "sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index - stub": { + "content": [ + { + "0": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ], + "csi": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-04T16:06:21.669668533" + }, + "sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_tbi - stub": { + "content": [ + { + "0": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:14:53.026083914" + }, "sarscov2 - [vcf, tbi], vcf, tsv, []": { "content": [ [ @@ -11,19 +221,60 @@ ] ], [ - "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + "versions.yml:md5,241125d00357804552689c37bbabe1f5" ] ], - "timestamp": "2024-02-05T17:12:20.799849895" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-31T15:15:14.663512924" }, "sarscov2 - [vcf, tbi], [], [], [] - stub": { "content": [ "out.vcf", [ - "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + "versions.yml:md5,241125d00357804552689c37bbabe1f5" ] ], - "timestamp": "2024-02-05T16:53:34.652746985" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-31T15:15:19.723448323" + }, + "sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index": { + "content": [ + [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.csi" + ] + ], + [ + + ], + [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T08:24:36.358469315" }, "sarscov2 - [vcf, tbi], [], [], []": { "content": [ @@ -37,9 +288,46 @@ ] ], [ - "versions.yml:md5,106d119dde844ec7fee1cdd30828bcdc" + "versions.yml:md5,241125d00357804552689c37bbabe1f5" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-31T15:15:09.588867653" + }, + "sarscov2 - [vcf, tbi], [], [], [] - vcf_gz_index_csi": { + "content": [ + [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "out", + "single_end": false + }, + "out_vcf.vcf.gz.csi" + ] + ], + [ + + ], + [ + "versions.yml:md5,241125d00357804552689c37bbabe1f5" ] ], - "timestamp": "2024-02-05T17:12:14.247465409" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:13:33.834986869" } } \ No newline at end of file diff --git a/modules/nf-core/bcftools/view/tests/vcf_gz_index.config b/modules/nf-core/bcftools/view/tests/vcf_gz_index.config new file mode 100644 index 0000000..7dd696e --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/vcf_gz_index.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index --no-version" +} diff --git a/modules/nf-core/bcftools/view/tests/vcf_gz_index_csi.config b/modules/nf-core/bcftools/view/tests/vcf_gz_index_csi.config new file mode 100644 index 0000000..aebffb6 --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/vcf_gz_index_csi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=csi --no-version" +} diff --git a/modules/nf-core/bcftools/view/tests/vcf_gz_index_tbi.config b/modules/nf-core/bcftools/view/tests/vcf_gz_index_tbi.config new file mode 100644 index 0000000..b192ae7 --- /dev/null +++ b/modules/nf-core/bcftools/view/tests/vcf_gz_index_tbi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=tbi --no-version" +} diff --git a/nextflow_schema.json b/nextflow_schema.json index fc4b2f4..5dd3d26 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -41,7 +41,7 @@ "enum": ["germline", "somatic"], "pattern": "(germline|somatic)", "fa_icon": "fas fa-folder-open", - "errorMessage": "Analysis type has to be choosen: germline or somatic" + "errorMessage": "Analysis type has to be chosen: germline or somatic" }, "variant_type": { "type": "string", @@ -78,9 +78,9 @@ "preprocess": { "type": "string", - "description": "The preprocessing steps to perform on the input files. Should be a comma-separated list of one or more of the following options: normalization, deduplication, prepy, filter_contigs", - "errorMessage": "A wrong input has been detected. It should be a comma-separated list of on or more of these options: normalization, deduplication, prepy, filter_contigs", - "pattern": "^((normalization|deduplication|prepy|filter_contigs)?,?)*(? 0 | params.max_sv_size != -1 | params.min_allele_freq != -1 | params.min_num_reads != -1 ){ + if (params.include_expression != null || params.exclude_expression != null || params.min_sv_size > 0 || params.max_sv_size != -1 || params.min_allele_freq != -1 || params.min_num_reads != -1 ){ - // Filters variants and SVs with given paramaters + // Filters variants and SVs with given parameters VCF_VARIANT_FILTERING( vcf_ch ) @@ -117,7 +111,7 @@ workflow PREPARE_VCFS_TEST { versions = versions.mix(VCF_VARIANT_FILTERING.out.versions.first()) } - if (params.preprocess.contains("deduplication")){ + if (params.preprocess.contains("deduplicate")){ // Deduplicate variants at the same position test VCF_VARIANT_DEDUPLICATION( @@ -129,6 +123,19 @@ workflow PREPARE_VCFS_TEST { } + if (params.preprocess.contains("normalize")){ + + // Turn on left alignment and m\normalization + BCFTOOLS_NORM( + vcf_ch, + fasta + ) + versions = versions.mix(BCFTOOLS_NORM.out.versions.first()) + + BCFTOOLS_NORM.out.vcf.join(BCFTOOLS_NORM.out.tbi, by:0) + .set{vcf_ch} + } + if (params.analysis.contains("somatic")){ // somatic specific preparations diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 1548741..18326ac 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -3,11 +3,11 @@ // -include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' -include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' include { VCF_REHEADER_SAMPLENAME } from '../local/vcf_reheader_samplename' include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' include { LIFTOVER_VCFS } from '../local/liftover_vcfs' +include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' +include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../modules/nf-core/bcftools/norm' workflow PREPARE_VCFS_TRUTH { @@ -48,25 +48,20 @@ workflow PREPARE_VCFS_TRUTH { versions = versions.mix(VCF_REHEADER_SAMPLENAME.out.versions.first()) vcf_ch = VCF_REHEADER_SAMPLENAME.out.ch_vcf - if (params.preprocess.contains("normalization")){ + if (params.preprocess.contains("split_multiallelic")){ - // multi-allelic variants will be splitter. - BCFTOOLS_NORM( + // Split -any- multi-allelic variants + BCFTOOLS_SPLIT_MULTI( vcf_ch, fasta ) - versions = versions.mix(BCFTOOLS_NORM.out.versions.first()) + versions = versions.mix(BCFTOOLS_SPLIT_MULTI.out.versions.first()) - // index vcf file - TABIX_TABIX( - BCFTOOLS_NORM.out.vcf - ) - versions = versions.mix(TABIX_TABIX.out.versions) - - BCFTOOLS_NORM.out.vcf.join(TABIX_TABIX.out.tbi, by:0) + BCFTOOLS_SPLIT_MULTI.out.vcf.join(BCFTOOLS_SPLIT_MULTI.out.tbi, by:0) .set{vcf_ch} } - if (params.preprocess.contains("deduplication")){ + + if (params.preprocess.contains("deduplicate")){ // Deduplicates variants at the same position test VCF_VARIANT_DEDUPLICATION( @@ -77,6 +72,19 @@ workflow PREPARE_VCFS_TRUTH { versions = versions.mix(VCF_VARIANT_DEDUPLICATION.out.versions) } + if (params.preprocess.contains("normalize")){ + + // Turn on left alignment and m\normalization + BCFTOOLS_NORM( + vcf_ch, + fasta + ) + versions = versions.mix(BCFTOOLS_NORM.out.versions.first()) + + BCFTOOLS_NORM.out.vcf.join(BCFTOOLS_NORM.out.tbi, by:0) + .set{vcf_ch} + } + emit: vcf_ch // channel: [val(meta), vcf, tbi] high_conf_ch // channel: [val(meta), bed] From fb6714fd3b5043415f7ffd401a8529565a578db8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 15 Jan 2025 14:52:54 +0000 Subject: [PATCH 238/292] update bcftools sort and reheader rm reheader subworkflow --- conf/modules.config | 4 +- conf/tests/germline_small.config | 2 +- conf/tests/germline_sv.config | 2 +- conf/tests/liftover_truth.config | 2 +- modules.json | 4 +- .../nf-core/bcftools/reheader/environment.yml | 4 +- modules/nf-core/bcftools/reheader/main.nf | 15 +- modules/nf-core/bcftools/reheader/meta.yml | 89 +++-- .../bcftools/reheader/tests/main.nf.test | 233 +++++++++++- .../bcftools/reheader/tests/main.nf.test.snap | 345 ++++++++++++++++-- .../reheader/tests/vcf_gz_index.config | 4 + .../reheader/tests/vcf_gz_index_csi.config | 4 + .../reheader/tests/vcf_gz_index_tbi.config | 5 + modules/nf-core/bcftools/sort/environment.yml | 4 +- modules/nf-core/bcftools/sort/main.nf | 19 +- modules/nf-core/bcftools/sort/meta.yml | 65 ++-- .../nf-core/bcftools/sort/tests/main.nf.test | 196 +++++++++- .../bcftools/sort/tests/main.nf.test.snap | 325 ++++++++++++++++- .../bcftools/sort/tests/vcf_gz_index.config | 4 + .../sort/tests/vcf_gz_index_csi.config | 4 + .../sort/tests/vcf_gz_index_tbi.config | 4 + subworkflows/local/prepare_vcfs_test.nf | 16 +- subworkflows/local/prepare_vcfs_truth.nf | 15 +- .../local/small_germline_benchmark.nf | 60 +-- subworkflows/local/sv_germline_benchmark.nf | 62 ++-- subworkflows/local/vcf_reheader_samplename.nf | 41 --- .../local/vcf_variant_deduplication.nf | 9 +- 27 files changed, 1302 insertions(+), 235 deletions(-) create mode 100644 modules/nf-core/bcftools/reheader/tests/vcf_gz_index.config create mode 100644 modules/nf-core/bcftools/reheader/tests/vcf_gz_index_csi.config create mode 100644 modules/nf-core/bcftools/reheader/tests/vcf_gz_index_tbi.config create mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index.config create mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config create mode 100644 modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config delete mode 100644 subworkflows/local/vcf_reheader_samplename.nf diff --git a/conf/modules.config b/conf/modules.config index 9e132a7..f85cce6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -59,7 +59,7 @@ process { } withName: "BCFTOOLS_SORT" { ext.prefix = { vcf.baseName - ".vcf" + ".sort"} - ext.args = {"--output-type z" } + ext.args = {"--output-type z --write-index=tbi" } publishDir = [ path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, pattern: "*{.vcf.gz}", @@ -73,7 +73,7 @@ process { " > ${meta.id}.txt" ].join('').trim()} ext.args = { "--samples ${meta.id}.txt" } - ext.args2 = {"--output-type z" } + ext.args2 = {"--output-type z --write-index=tbi" } ext.prefix = { vcf.baseName - ".vcf" + ".rh"} } // splitting tools diff --git a/conf/tests/germline_small.config b/conf/tests/germline_small.config index 871751a..1644408 100644 --- a/conf/tests/germline_small.config +++ b/conf/tests/germline_small.config @@ -33,7 +33,7 @@ params { analysis = 'germline' variant_type = "small" method = 'happy,rtgtools' - preprocess = "normalization,deduplication,prepy" + preprocess = "normalize,deduplicate,prepy" include_expression = '(ILEN >= -5 && ILEN <= 5)' // truth information diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config index dc724f3..18d2729 100644 --- a/conf/tests/germline_sv.config +++ b/conf/tests/germline_sv.config @@ -33,7 +33,7 @@ params { analysis = 'germline' variant_type = "structural" method = 'svanalyzer,wittyer,truvari' - preprocess = "normalization,deduplication,filter_contigs" + preprocess = "normalize,deduplicate,filter_contigs" sv_standardization = "svync,homogenize" min_sv_size = 30 truth_id = "HG002" diff --git a/conf/tests/liftover_truth.config b/conf/tests/liftover_truth.config index 3ab7d01..de1b854 100644 --- a/conf/tests/liftover_truth.config +++ b/conf/tests/liftover_truth.config @@ -29,7 +29,7 @@ params { truth_id = "HG002" variant_type = "small" method = 'rtgtools,happy' - preprocess = "normalization,deduplication,filter_contigs" + preprocess = "split_multiallelic,deduplicate,filter_contigs" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" diff --git a/modules.json b/modules.json index 1020492..ac2a4b9 100644 --- a/modules.json +++ b/modules.json @@ -32,12 +32,12 @@ }, "bcftools/reheader": { "branch": "master", - "git_sha": "44096c08ffdbc694f5f92ae174ea0f7ba0f37e09", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "bcftools/sort": { "branch": "master", - "git_sha": "487d92367b4d7bb9f1ca694bf72736be90720b15", + "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", "installed_by": ["modules"] }, "bcftools/stats": { diff --git a/modules/nf-core/bcftools/reheader/environment.yml b/modules/nf-core/bcftools/reheader/environment.yml index aab0dc9..5c00b11 100644 --- a/modules/nf-core/bcftools/reheader/environment.yml +++ b/modules/nf-core/bcftools/reheader/environment.yml @@ -1,7 +1,5 @@ -name: bcftools_reheader channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::bcftools=1.18 + - bioconda::bcftools=1.20 diff --git a/modules/nf-core/bcftools/reheader/main.nf b/modules/nf-core/bcftools/reheader/main.nf index 8252716..9cf6d0d 100644 --- a/modules/nf-core/bcftools/reheader/main.nf +++ b/modules/nf-core/bcftools/reheader/main.nf @@ -4,8 +4,8 @@ process BCFTOOLS_REHEADER { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'biocontainers/bcftools:1.18--h8b25389_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.20--h8b25389_0': + 'biocontainers/bcftools:1.20--h8b25389_0' }" input: tuple val(meta), path(vcf), path(header), path(samples) @@ -13,6 +13,7 @@ process BCFTOOLS_REHEADER { output: tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + tuple val(meta), path("*.{csi,tbi}") , emit: index, optional: true path "versions.yml" , emit: versions when: @@ -59,8 +60,16 @@ process BCFTOOLS_REHEADER { args2.contains("--output-type z") || args2.contains("-Oz") ? "vcf.gz" : args2.contains("--output-type v") || args2.contains("-Ov") ? "vcf" : "vcf" + def index = args2.contains("--write-index=tbi") || args2.contains("-W=tbi") ? "tbi" : + args2.contains("--write-index=csi") || args2.contains("-W=csi") ? "csi" : + args2.contains("--write-index") || args2.contains("-W") ? "csi" : + "" + def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch" + def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : "" + """ - touch ${prefix}.${extension} + ${create_cmd} ${prefix}.${extension} + ${create_index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bcftools/reheader/meta.yml b/modules/nf-core/bcftools/reheader/meta.yml index 690d4ea..47e5344 100644 --- a/modules/nf-core/bcftools/reheader/meta.yml +++ b/modules/nf-core/bcftools/reheader/meta.yml @@ -12,47 +12,60 @@ tools: documentation: http://samtools.github.io/bcftools/bcftools.html#reheader doi: 10.1093/gigascience/giab008 licence: ["MIT"] + identifier: biotools:bcftools input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: VCF/BCF file - pattern: "*.{vcf.gz,vcf,bcf}" - - header: - type: file - description: New header to add to the VCF - pattern: "*.{header.txt}" - - samples: - type: file - description: File containing sample names to update (one sample per line) - pattern: "*.{samples.txt}" - - meta2: - type: map - description: | - Groovy Map containing reference information - e.g. [ id:'genome' ] - - fai: - type: file - description: Fasta index to update header sequences with - pattern: "*.{fai}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF/BCF file + pattern: "*.{vcf.gz,vcf,bcf}" + - header: + type: file + description: New header to add to the VCF + pattern: "*.{header.txt}" + - samples: + type: file + description: File containing sample names to update (one sample per line) + pattern: "*.{samples.txt}" + - - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] + - fai: + type: file + description: Fasta index to update header sequences with + pattern: "*.{fai}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - vcf: - type: file - description: VCF with updated header, bgzipped per default - pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.{vcf,vcf.gz,bcf,bcf.gz}": + type: file + description: VCF with updated header, bgzipped per default + pattern: "*.{vcf,vcf.gz,bcf,bcf.gz}" + - index: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.{csi,tbi}": + type: file + description: Index of VCF with updated header + pattern: "*.{csi,tbi}" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@bjohnnyd" - "@jemten" diff --git a/modules/nf-core/bcftools/reheader/tests/main.nf.test b/modules/nf-core/bcftools/reheader/tests/main.nf.test index f3200cb..96c1b7b 100644 --- a/modules/nf-core/bcftools/reheader/tests/main.nf.test +++ b/modules/nf-core/bcftools/reheader/tests/main.nf.test @@ -17,13 +17,13 @@ nextflow_process { """ input[0] = [ [ id:'test', single_end:false ], - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), [], [] ] - input[1] = [ + input[1] = [ [ id:'genome' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ] """ } @@ -47,13 +47,13 @@ nextflow_process { """ input[0] = [ [ id:'test', single_end:false ], - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), [], [] ] - input[1] = [ + input[1] = [ [ id:'genome' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ] """ } @@ -68,6 +68,111 @@ nextflow_process { } + test("sarscov2 - [vcf, [], []], fai - vcf.gz output - index") { + + config "./vcf_gz_index.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.index.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.index[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - vcf.gz output - csi index") { + + config "./vcf_gz_index_csi.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.index.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.index[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - vcf.gz output - tbi index") { + + config "./vcf_gz_index_tbi.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.index.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.index[0][1].endsWith(".tbi") } + ) + } + + } + test("sarscov2 - [vcf, [], []], fai - bcf output") { config "./bcf.config" @@ -77,13 +182,13 @@ nextflow_process { """ input[0] = [ [ id:'test', single_end:false ], - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), [], [] ] - input[1] = [ + input[1] = [ [ id:'genome' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ] """ } @@ -107,11 +212,11 @@ nextflow_process { """ input[0] = [ [ id:'test', single_end:false ], - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true), [] ] - input[1] = [ + input[1] = [ [ id:'genome' ], // meta map [] ] @@ -137,15 +242,15 @@ nextflow_process { """ ch_no_samples = Channel.of([ [ id:'test', single_end:false ], - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), [] ]) ch_samples = Channel.of(["samples.txt", "new_name"]) .collectFile(newLine:true) input[0] = ch_no_samples.combine(ch_samples) - input[1] = [ + input[1] = [ [ id:'genome' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ] """ } @@ -170,13 +275,13 @@ nextflow_process { """ input[0] = [ [ id:'test', single_end:false ], - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), [], [] ] - input[1] = [ + input[1] = [ [ id:'genome' ], // meta map - file(params.test_data['sarscov2']['genome']['genome_fasta_fai'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) ] """ } @@ -193,5 +298,97 @@ nextflow_process { } } + test("sarscov2 - [vcf, [], []], fai - vcf.gz output - index -stub") { + + options "-stub" + config "./vcf_gz_index.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - vcf.gz output - csi index -stub") { + + options "-stub" + config "./vcf_gz_index_csi.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - [vcf, [], []], fai - vcf.gz output - tbi index -stub") { + + options "-stub" + config "./vcf_gz_index_tbi.config" + when { + + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf.gz', checkIfExists: true), + [], + [] + ] + input[1] = [ + [ id:'genome' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } } diff --git a/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap b/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap index 112736a..87a3654 100644 --- a/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap +++ b/modules/nf-core/bcftools/reheader/tests/main.nf.test.snap @@ -1,4 +1,140 @@ { + "sarscov2 - [vcf, [], []], fai - vcf.gz output - tbi index": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.tbi" + ] + ], + [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T10:09:05.955833763" + }, + "sarscov2 - [vcf, [], []], fai - vcf.gz output - index -stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:52:41.444952182" + }, + "sarscov2 - [vcf, [], []], fai - vcf.gz output - tbi index -stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:53:04.314827944" + }, "sarscov2 - [vcf, [], []], fai - vcf output": { "content": [ { @@ -12,7 +148,13 @@ ] ], "1": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + ], "vcf": [ [ @@ -24,11 +166,15 @@ ] ], "versions": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" ] } ], - "timestamp": "2023-11-29T13:05:44.058376693" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:50:41.983008108" }, "sarscov2 - [vcf, [], []], fai - bcf output": { "content": [ @@ -39,11 +185,17 @@ "id": "test", "single_end": false }, - "tested.bcf.gz:md5,c31d9afd8614832c2a46d9a55682c97a" + "tested.bcf.gz:md5,c8a304c8d2892039201154153c8cd536" ] ], "1": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + ], "vcf": [ [ @@ -51,15 +203,19 @@ "id": "test", "single_end": false }, - "tested.bcf.gz:md5,c31d9afd8614832c2a46d9a55682c97a" + "tested.bcf.gz:md5,c8a304c8d2892039201154153c8cd536" ] ], "versions": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" ] } ], - "timestamp": "2023-11-29T13:06:03.793372514" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:51:43.072513252" }, "sarscov2 - [vcf, [], []], fai - vcf.gz output": { "content": [ @@ -70,11 +226,17 @@ "id": "test", "single_end": false }, - "tested.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + "tested.vcf.gz:md5,8e722884ffb75155212a3fc053918766" ] ], "1": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + ], "vcf": [ [ @@ -82,24 +244,145 @@ "id": "test", "single_end": false }, - "tested.vcf.gz:md5,a1e45fe6d2b386fc2611766e5d2937ee" + "tested.vcf.gz:md5,8e722884ffb75155212a3fc053918766" ] ], "versions": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" ] } ], - "timestamp": "2023-11-29T13:05:53.954090441" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:50:53.055630152" + }, + "sarscov2 - [vcf, [], []], fai - vcf.gz output - index": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.csi" + ] + ], + [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T10:08:37.999924355" + }, + "sarscov2 - [vcf, [], []], fai - vcf.gz output - csi index -stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:52:52.512269206" }, "sarscov2 - [vcf, [], []], fai - stub": { "content": [ "tested.vcf", [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" ] ], - "timestamp": "2023-11-29T13:06:33.549685303" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-05-31T15:16:36.337112514" + }, + "sarscov2 - [vcf, [], []], fai - vcf.gz output - csi index": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test_vcf.vcf.gz.csi" + ] + ], + [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T10:08:55.434831174" }, "sarscov2 - [vcf, [], samples], fai": { "content": [ @@ -114,7 +397,13 @@ ] ], "1": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + ], "vcf": [ [ @@ -126,11 +415,15 @@ ] ], "versions": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" ] } ], - "timestamp": "2023-11-29T13:06:23.474745156" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:52:12.216002665" }, "sarscov2 - [vcf, header, []], []": { "content": [ @@ -145,7 +438,13 @@ ] ], "1": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + + ], + "2": [ + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" + ], + "index": [ + ], "vcf": [ [ @@ -157,10 +456,14 @@ ] ], "versions": [ - "versions.yml:md5,fbf8ac8da771b6295a47392003f983ce" + "versions.yml:md5,486e3d4ebc1dbf5c0a4dfaebae12ea34" ] } ], - "timestamp": "2023-11-29T13:06:13.841648691" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-09-03T09:51:54.062386022" } } \ No newline at end of file diff --git a/modules/nf-core/bcftools/reheader/tests/vcf_gz_index.config b/modules/nf-core/bcftools/reheader/tests/vcf_gz_index.config new file mode 100644 index 0000000..1e050ec --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/vcf_gz_index.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args2 = "--output-type z --write-index --no-version" +} diff --git a/modules/nf-core/bcftools/reheader/tests/vcf_gz_index_csi.config b/modules/nf-core/bcftools/reheader/tests/vcf_gz_index_csi.config new file mode 100644 index 0000000..536e4b4 --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/vcf_gz_index_csi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args2 = "--output-type z --write-index=csi --no-version" +} diff --git a/modules/nf-core/bcftools/reheader/tests/vcf_gz_index_tbi.config b/modules/nf-core/bcftools/reheader/tests/vcf_gz_index_tbi.config new file mode 100644 index 0000000..91a80db --- /dev/null +++ b/modules/nf-core/bcftools/reheader/tests/vcf_gz_index_tbi.config @@ -0,0 +1,5 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args2 = "--output-type z --write-index=tbi --no-version" + +} diff --git a/modules/nf-core/bcftools/sort/environment.yml b/modules/nf-core/bcftools/sort/environment.yml index 89cf911..5c00b11 100644 --- a/modules/nf-core/bcftools/sort/environment.yml +++ b/modules/nf-core/bcftools/sort/environment.yml @@ -1,7 +1,5 @@ -name: bcftools_sort channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::bcftools=1.18 + - bioconda::bcftools=1.20 diff --git a/modules/nf-core/bcftools/sort/main.nf b/modules/nf-core/bcftools/sort/main.nf index c982944..7d4c9b8 100644 --- a/modules/nf-core/bcftools/sort/main.nf +++ b/modules/nf-core/bcftools/sort/main.nf @@ -4,15 +4,17 @@ process BCFTOOLS_SORT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.18--h8b25389_0': - 'biocontainers/bcftools:1.18--h8b25389_0' }" + 'https://depot.galaxyproject.org/singularity/bcftools:1.20--h8b25389_0': + 'biocontainers/bcftools:1.20--h8b25389_0' }" input: tuple val(meta), path(vcf) output: - tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}") , emit: vcf - path "versions.yml" , emit: versions + tuple val(meta), path("*.{vcf,vcf.gz,bcf,bcf.gz}"), emit: vcf + tuple val(meta), path("*.tbi") , emit: tbi, optional: true + tuple val(meta), path("*.csi") , emit: csi, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -49,9 +51,16 @@ process BCFTOOLS_SORT { args.contains("--output-type z") || args.contains("-Oz") ? "vcf.gz" : args.contains("--output-type v") || args.contains("-Ov") ? "vcf" : "vcf" + def index = args.contains("--write-index=tbi") || args.contains("-W=tbi") ? "tbi" : + args.contains("--write-index=csi") || args.contains("-W=csi") ? "csi" : + args.contains("--write-index") || args.contains("-W") ? "csi" : + "" + def create_cmd = extension.endsWith(".gz") ? "echo '' | gzip >" : "touch" + def create_index = extension.endsWith(".gz") && index.matches("csi|tbi") ? "touch ${prefix}.${extension}.${index}" : "" """ - touch ${prefix}.${extension} + ${create_cmd} ${prefix}.${extension} + ${create_index} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bcftools/sort/meta.yml b/modules/nf-core/bcftools/sort/meta.yml index 84747c6..f7a6eff 100644 --- a/modules/nf-core/bcftools/sort/meta.yml +++ b/modules/nf-core/bcftools/sort/meta.yml @@ -12,30 +12,53 @@ tools: tool_dev_url: https://github.com/samtools/bcftools doi: "10.1093/bioinformatics/btp352" licence: ["MIT"] + identifier: biotools:bcftools input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: The VCF/BCF file to be sorted - pattern: "*.{vcf.gz,vcf,bcf}" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: The VCF/BCF file to be sorted + pattern: "*.{vcf.gz,vcf,bcf}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - vcf: - type: file - description: Sorted VCF file - pattern: "*.{vcf.gz}" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.{vcf,vcf.gz,bcf,bcf.gz}": + type: file + description: Sorted VCF file + pattern: "*.{vcf.gz}" + - tbi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.tbi": + type: file + description: Alternative VCF file index + pattern: "*.tbi" + - csi: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.csi": + type: file + description: Default VCF file index + pattern: "*.csi" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@Gwennid" maintainers: diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test b/modules/nf-core/bcftools/sort/tests/main.nf.test index fec59cf..b9bdd76 100644 --- a/modules/nf-core/bcftools/sort/tests/main.nf.test +++ b/modules/nf-core/bcftools/sort/tests/main.nf.test @@ -9,13 +9,125 @@ nextflow_process { tag "bcftools" tag "bcftools/sort" - test("SarsCov2 VCF") { + test("sarscov2 - vcf") { when { process { """ input[0] = [ [ id:'test' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("vcf") } + ) + } + + } + + test("sarscov2 - vcf_gz_index") { + + config "./vcf_gz_index.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_csi") { + + config "./vcf_gz_index_csi.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_tbi") { + + config "./vcf_gz_index_tbi.config" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf, + process.out.csi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() }, + { assert process.out.tbi[0][1].endsWith(".tbi") } + ) + } + + } + + test("sarscov2 - vcf - stub") { + options "-stub" + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) ] """ } @@ -29,4 +141,82 @@ nextflow_process { } } -} + + test("sarscov2 - vcf_gz_index - stub") { + + config "./vcf_gz_index.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_csi - stub") { + + config "./vcf_gz_index_csi.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.csi[0][1].endsWith(".csi") } + ) + } + + } + + test("sarscov2 - vcf_gz_index_tbi - stub") { + + config "./vcf_gz_index_tbi.config" + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/vcf/test.vcf', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.tbi[0][1].endsWith(".tbi") } + ) + } + + } +} \ No newline at end of file diff --git a/modules/nf-core/bcftools/sort/tests/main.nf.test.snap b/modules/nf-core/bcftools/sort/tests/main.nf.test.snap index 7f59955..f38272c 100644 --- a/modules/nf-core/bcftools/sort/tests/main.nf.test.snap +++ b/modules/nf-core/bcftools/sort/tests/main.nf.test.snap @@ -1,5 +1,60 @@ { - "SarsCov2 VCF": { + "sarscov2 - vcf_gz_index_tbi - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ], + "csi": [ + + ], + "tbi": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:06:05.201680777" + }, + "vcf": { "content": [ { "0": [ @@ -11,7 +66,19 @@ ] ], "1": [ - "versions.yml:md5,622bd32d4ff0fac3360cd534ae0f0168" + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ], + "csi": [ + + ], + "tbi": [ + ], "vcf": [ [ @@ -22,14 +89,262 @@ ] ], "versions": [ - "versions.yml:md5,622bd32d4ff0fac3360cd534ae0f0168" + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" ] } ], "meta": { "nf-test": "0.8.4", - "nextflow": "23.04.2" + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:04:43.889971134" + }, + "sarscov2 - vcf_gz_index": { + "content": [ + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.csi" + ] + ], + [ + + ], + [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:04:55.385964497" + }, + "sarscov2 - vcf_gz_index_csi": { + "content": [ + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.csi" + ] + ], + [ + + ], + [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:05:06.662818922" + }, + "sarscov2 - vcf_gz_index - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ], + "csi": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:05:40.012912381" + }, + "sarscov2 - vcf_gz_index_csi - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ], + "csi": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.csi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:05:52.405673587" + }, + "sarscov2 - vcf - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ], + "csi": [ + + ], + "tbi": [ + + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-05T12:05:29.117946461" + }, + "sarscov2 - vcf_gz_index_tbi": { + "content": [ + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz:md5,8e722884ffb75155212a3fc053918766" + ] + ], + [ + + ], + [ + [ + { + "id": "test" + }, + "test_vcf.vcf.gz.tbi" + ] + ], + [ + "versions.yml:md5,2c9f26ca356ef71199c3a7d1742974cb" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" }, - "timestamp": "2024-03-18T12:50:10.340362246" + "timestamp": "2024-06-05T12:05:17.217274984" } } \ No newline at end of file diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config new file mode 100644 index 0000000..aacd134 --- /dev/null +++ b/modules/nf-core/bcftools/sort/tests/vcf_gz_index.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index" +} diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config new file mode 100644 index 0000000..640eb0b --- /dev/null +++ b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_csi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=csi" +} diff --git a/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config new file mode 100644 index 0000000..589a50c --- /dev/null +++ b/modules/nf-core/bcftools/sort/tests/vcf_gz_index_tbi.config @@ -0,0 +1,4 @@ +process { + ext.prefix = { "${meta.id}_vcf" } + ext.args = "--output-type z --write-index=tbi" +} diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index d62c0ff..01a169b 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -2,7 +2,7 @@ // PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS // -include { VCF_REHEADER_SAMPLENAME } from '../local/vcf_reheader_samplename' +include { BCFTOOLS_REHEADER } from '../../modules/nf-core/bcftools/reheader' include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' include { VCF_VARIANT_FILTERING } from '../local/vcf_variant_filtering' include { SPLIT_SMALL_VARIANTS_TEST } from '../local/split_small_variants_test' @@ -68,12 +68,18 @@ workflow PREPARE_VCFS_TEST { // Add "query" to test sample - VCF_REHEADER_SAMPLENAME( - vcf_ch, + // rename sample name + BCFTOOLS_REHEADER( + + vcf_ch.map{ meta, file -> + [ meta, file, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME.out.versions.first()) - vcf_ch = VCF_REHEADER_SAMPLENAME.out.ch_vcf + versions = versions.mix(BCFTOOLS_REHEADER.out.versions.first()) + + BCFTOOLS_REHEADER.out.vcf.join(BCFTOOLS_REHEADER.out.index) + .set{vcf_ch} if (params.preprocess.contains("filter_contigs")){ // filter out extra contigs! diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 18326ac..22ba168 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -3,7 +3,7 @@ // -include { VCF_REHEADER_SAMPLENAME } from '../local/vcf_reheader_samplename' +include { BCFTOOLS_REHEADER } from '../../modules/nf-core/bcftools/reheader' include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' include { LIFTOVER_VCFS } from '../local/liftover_vcfs' include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' @@ -41,12 +41,17 @@ workflow PREPARE_VCFS_TRUTH { } // Reheader sample name for truth file - using meta.caller - VCF_REHEADER_SAMPLENAME( - truth_ch, + // rename sample name + BCFTOOLS_REHEADER( + truth_ch.map{ meta, file -> + [ meta, file, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME.out.versions.first()) - vcf_ch = VCF_REHEADER_SAMPLENAME.out.ch_vcf + versions = versions.mix(BCFTOOLS_REHEADER.out.versions.first()) + + BCFTOOLS_REHEADER.out.vcf.join(BCFTOOLS_REHEADER.out.index) + .set{vcf_ch} if (params.preprocess.contains("split_multiallelic")){ diff --git a/subworkflows/local/small_germline_benchmark.nf b/subworkflows/local/small_germline_benchmark.nf index 5e07217..c8c6ab6 100644 --- a/subworkflows/local/small_germline_benchmark.nf +++ b/subworkflows/local/small_germline_benchmark.nf @@ -2,14 +2,14 @@ // SMALL_GERMLINE_BENCHMARK: SUBWORKFLOW FOR SMALL GERMLINE VARIANTS // -include { RTGTOOLS_FORMAT } from '../../modules/nf-core/rtgtools/format/main' -include { RTGTOOLS_VCFEVAL } from '../../modules/nf-core/rtgtools/vcfeval/main' -include { HAPPY_HAPPY } from '../../modules/nf-core/happy/happy/main' -include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_1 } from '../local/vcf_reheader_samplename' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_2 } from '../local/vcf_reheader_samplename' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_3 } from '../local/vcf_reheader_samplename' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_4 } from '../local/vcf_reheader_samplename' +include { RTGTOOLS_FORMAT } from '../../modules/nf-core/rtgtools/format/main' +include { RTGTOOLS_VCFEVAL } from '../../modules/nf-core/rtgtools/vcfeval/main' +include { HAPPY_HAPPY } from '../../modules/nf-core/happy/happy/main' +include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_1 } from '../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_2 } from '../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_3 } from '../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_4 } from '../../modules/nf-core/bcftools/reheader' workflow SMALL_GERMLINE_BENCHMARK { take: @@ -55,43 +55,55 @@ workflow SMALL_GERMLINE_BENCHMARK { summary_reports = summary_reports.mix(report) // reheader benchmarking results properly and tag meta - VCF_REHEADER_SAMPLENAME_1( - RTGTOOLS_VCFEVAL.out.fn_vcf, + BCFTOOLS_REHEADER_1( + RTGTOOLS_VCFEVAL.out.fn_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_1.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_1.out.versions.first()) - VCF_REHEADER_SAMPLENAME_1.out.ch_vcf + BCFTOOLS_REHEADER_1.out.vcf + .join(BCFTOOLS_REHEADER_1.out.index) .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "rtgtools"], file, index) } .set { vcf_fn } - VCF_REHEADER_SAMPLENAME_2( - RTGTOOLS_VCFEVAL.out.fp_vcf, + BCFTOOLS_REHEADER_2( + RTGTOOLS_VCFEVAL.out.fp_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_2.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_2.out.versions) - VCF_REHEADER_SAMPLENAME_2.out.ch_vcf + BCFTOOLS_REHEADER_2.out.vcf + .join(BCFTOOLS_REHEADER_2.out.index) .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "rtgtools"], file, index) } .set { vcf_fp } - VCF_REHEADER_SAMPLENAME_3( - RTGTOOLS_VCFEVAL.out.baseline_vcf, + BCFTOOLS_REHEADER_3( + RTGTOOLS_VCFEVAL.out.baseline_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_3.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_3.out.versions) - VCF_REHEADER_SAMPLENAME_3.out.ch_vcf + BCFTOOLS_REHEADER_3.out.vcf + .join(BCFTOOLS_REHEADER_3.out.index) .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "rtgtools"], file, index) } .set { vcf_tp_base } - VCF_REHEADER_SAMPLENAME_4( - RTGTOOLS_VCFEVAL.out.tp_vcf, + BCFTOOLS_REHEADER_4( + RTGTOOLS_VCFEVAL.out.tp_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_4.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_4.out.versions) - VCF_REHEADER_SAMPLENAME_4.out.ch_vcf + BCFTOOLS_REHEADER_4.out.vcf + .join(BCFTOOLS_REHEADER_4.out.index) .map { _meta, file, index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "rtgtools"], file, index) } .set { vcf_tp_comp } diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark.nf index 780265c..1ad0b3b 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark.nf @@ -2,15 +2,15 @@ // SV_GERMLINE_BENCHMARK: SUBWORKFLOW FOR SV GERMLINE VARIANTS // -include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' -include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' -include { WITTYER } from '../../modules/nf-core/wittyer' -include { TABIX_BGZIP as TABIX_BGZIP_QUERY } from '../../modules/nf-core/tabix/bgzip' -include { TABIX_BGZIP as TABIX_BGZIP_TRUTH } from '../../modules/nf-core/tabix/bgzip' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_1 } from '../local/vcf_reheader_samplename' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_2 } from '../local/vcf_reheader_samplename' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_3 } from '../local/vcf_reheader_samplename' -include { VCF_REHEADER_SAMPLENAME as VCF_REHEADER_SAMPLENAME_4 } from '../local/vcf_reheader_samplename' +include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' +include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' +include { WITTYER } from '../../modules/nf-core/wittyer' +include { TABIX_BGZIP as TABIX_BGZIP_QUERY } from '../../modules/nf-core/tabix/bgzip' +include { TABIX_BGZIP as TABIX_BGZIP_TRUTH } from '../../modules/nf-core/tabix/bgzip' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_1 } from '../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_2 } from '../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_3 } from '../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_4 } from '../../modules/nf-core/bcftools/reheader' workflow SV_GERMLINE_BENCHMARK { take: @@ -43,49 +43,61 @@ workflow SV_GERMLINE_BENCHMARK { summary_reports = summary_reports.mix(report) // reheader fn vcf files for tagged results - VCF_REHEADER_SAMPLENAME_1( - TRUVARI_BENCH.out.fn_vcf, + BCFTOOLS_REHEADER_1( + TRUVARI_BENCH.out.fn_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_1.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_1.out.versions) - VCF_REHEADER_SAMPLENAME_1.out.ch_vcf + BCFTOOLS_REHEADER_1.out.vcf + .join(BCFTOOLS_REHEADER_1.out.index) .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "FN"] + [id: "truvari"], file) } .set { vcf_fn } // reheader fp vcf files for tagged results - VCF_REHEADER_SAMPLENAME_2( - TRUVARI_BENCH.out.fp_vcf, + BCFTOOLS_REHEADER_2( + TRUVARI_BENCH.out.fp_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_2.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_2.out.versions) // add tag and to meta - VCF_REHEADER_SAMPLENAME_2.out.ch_vcf + BCFTOOLS_REHEADER_2.out.vcf + .join(BCFTOOLS_REHEADER_2.out.index) .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "FP"] + [id: "truvari"], file) } .set { vcf_fp } // reheader base tp vcf files for tagged results - VCF_REHEADER_SAMPLENAME_3( - TRUVARI_BENCH.out.tp_base_vcf, + BCFTOOLS_REHEADER_3( + TRUVARI_BENCH.out.tp_base_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_3.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_3.out.versions) // add tag and to meta - VCF_REHEADER_SAMPLENAME_3.out.ch_vcf + BCFTOOLS_REHEADER_3.out.vcf + .join(BCFTOOLS_REHEADER_3.out.index) .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "TP_base"] + [id: "truvari"], file) } .set { vcf_tp_base } // reheader comp tp vcf files for tagged results - VCF_REHEADER_SAMPLENAME_4( - TRUVARI_BENCH.out.tp_comp_vcf, + BCFTOOLS_REHEADER_4( + TRUVARI_BENCH.out.tp_comp_vcf.map{ meta, vcf -> + [ meta, vcf, [], [] ] + }, fai ) - versions = versions.mix(VCF_REHEADER_SAMPLENAME_4.out.versions) + versions = versions.mix(BCFTOOLS_REHEADER_4.out.versions) // add tag and to meta - VCF_REHEADER_SAMPLENAME_4.out.ch_vcf + BCFTOOLS_REHEADER_4.out.vcf + .join(BCFTOOLS_REHEADER_4.out.index) .map { _meta, file, _index -> tuple([vartype: params.variant_type] + [tag: "TP_comp"] + [id: "truvari"], file) } .set { vcf_tp_comp } diff --git a/subworkflows/local/vcf_reheader_samplename.nf b/subworkflows/local/vcf_reheader_samplename.nf deleted file mode 100644 index 5d4384d..0000000 --- a/subworkflows/local/vcf_reheader_samplename.nf +++ /dev/null @@ -1,41 +0,0 @@ -// -// VCF_REHEADER_SAMPLENAME: reheader sample names when needed -// - -include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' -include { BCFTOOLS_REHEADER } from '../../modules/nf-core/bcftools/reheader' - -workflow VCF_REHEADER_SAMPLENAME { - take: - vcf_ch // channel: [val(meta), vcf] - fai // reference channel [val(meta), ref.fai] - - main: - - versions = Channel.empty() - - // rename sample name - BCFTOOLS_REHEADER( - - vcf_ch.map{ meta, vcf -> - [ meta, vcf, [], [] ] - }, - fai - ) - versions = versions.mix(BCFTOOLS_REHEADER.out.versions.first()) - - TABIX_TABIX( - BCFTOOLS_REHEADER.out.vcf - ) - versions = versions.mix(TABIX_TABIX.out.versions.first()) - - BCFTOOLS_REHEADER.out.vcf - .join(TABIX_TABIX.out.tbi, failOnDuplicate:true, failOnMismatch:true) - .set{ch_vcf} - - - emit: - ch_vcf // channel: [val(meta), vcf, index ] - versions // channel: [versions.yml ] - -} diff --git a/subworkflows/local/vcf_variant_deduplication.nf b/subworkflows/local/vcf_variant_deduplication.nf index 81b7496..b6331e1 100644 --- a/subworkflows/local/vcf_variant_deduplication.nf +++ b/subworkflows/local/vcf_variant_deduplication.nf @@ -3,7 +3,6 @@ // include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' -include { TABIX_TABIX } from '../../modules/nf-core/tabix/tabix' include { BCFTOOLS_NORM as BCFTOOLS_DEDUP } from '../../modules/nf-core/bcftools/norm' workflow VCF_VARIANT_DEDUPLICATION { @@ -29,13 +28,7 @@ workflow VCF_VARIANT_DEDUPLICATION { ) versions = versions.mix(BCFTOOLS_SORT.out.versions.first()) - TABIX_TABIX( - BCFTOOLS_SORT.out.vcf - ) - versions = versions.mix(TABIX_TABIX.out.versions.first()) - - BCFTOOLS_SORT.out.vcf - .join(TABIX_TABIX.out.tbi, failOnDuplicate:true, failOnMismatch:true) + BCFTOOLS_SORT.out.vcf.join(BCFTOOLS_SORT.out.tbi) .set{ch_vcf} emit: From 76d93a8da5124c7a8e3434a5583cfc7f2ef3e94b Mon Sep 17 00:00:00 2001 From: EladH1 Date: Fri, 17 Jan 2025 10:57:51 +0100 Subject: [PATCH 239/292] fix small test relative path --- workflows/variantbenchmarking.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 0d6ee56..5714c78 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -81,11 +81,11 @@ workflow VARIANTBENCHMARKING { }else{ if (params.genome == "GRCh38"){ - rename_chr = Channel.fromPath("assets/rename_contigs/grch37_grch38.txt", checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() + rename_chr = Channel.fromPath("${projectDir}/assets/rename_contigs/grch37_grch38.txt", checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() } else if(params.genome == "GRCh37") { - rename_chr = Channel.fromPath("assets/rename_contigs/grch38_grch37.txt", checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() + rename_chr = Channel.fromPath("${projectDir}/assets/rename_contigs/grch38_grch37.txt", checkIfExists: true).map{ txt -> tuple([id: txt.getSimpleName()], txt) }.collect() } else{ rename_chr = Channel.empty() From decb747b2bd08e441c60697c66e3d1df09b96d8b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 17 Jan 2025 10:07:15 +0000 Subject: [PATCH 240/292] modify output names update readme --- README.md | 55 +-- conf/modules.config | 327 +++++++++++------- conf/test.config | 4 +- conf/tests/liftover_truth.config | 2 +- docs/output.md | 4 +- docs/usage.md | 18 +- main.nf | 2 +- .../publish_processed_vcf/environment.yml | 8 + .../custom/publish_processed_vcf/main.nf | 35 ++ .../nf-core/bcftools/query/environment.yml | 7 - modules/nf-core/bcftools/query/main.nf | 56 --- modules/nf-core/bcftools/query/meta.yml | 63 ---- .../nf-core/bcftools/query/tests/main.nf.test | 101 ------ .../bcftools/query/tests/main.nf.test.snap | 55 --- .../bcftools/query/tests/nextflow.config | 3 - modules/nf-core/bcftools/query/tests/tags.yml | 2 - nextflow.config | 2 +- .../local/compare_benchmark_results.nf | 8 +- subworkflows/local/prepare_vcfs_test.nf | 21 +- subworkflows/local/prepare_vcfs_truth.nf | 14 +- .../local/split_small_variants_test.nf | 16 +- tests/germline_small.nf.test.snap | 130 ++++--- tests/germline_sv.nf.test.snap | 138 ++++---- tests/liftover_test.nf.test.snap | 55 +-- tests/liftover_truth.nf.test.snap | 84 +++-- tests/somatic_indel.nf.test.snap | 37 +- tests/somatic_snv.nf.test.snap | 35 +- tests/somatic_sv.nf.test.snap | 77 +++-- 28 files changed, 650 insertions(+), 709 deletions(-) create mode 100644 modules/local/custom/publish_processed_vcf/environment.yml create mode 100644 modules/local/custom/publish_processed_vcf/main.nf delete mode 100644 modules/nf-core/bcftools/query/environment.yml delete mode 100644 modules/nf-core/bcftools/query/main.nf delete mode 100644 modules/nf-core/bcftools/query/meta.yml delete mode 100644 modules/nf-core/bcftools/query/tests/main.nf.test delete mode 100644 modules/nf-core/bcftools/query/tests/main.nf.test.snap delete mode 100644 modules/nf-core/bcftools/query/tests/nextflow.config delete mode 100644 modules/nf-core/bcftools/query/tests/tags.yml diff --git a/README.md b/README.md index 39daff6..e68c04e 100644 --- a/README.md +++ b/README.md @@ -39,31 +39,32 @@ This initial step ensures consistent formatting and alignment of variants in tes 4. Rename sample names in test and truth VCF files ([bcftools reheader](https://samtools.github.io/bcftools/bcftools.html#reheader)) 5. Splitting multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) 6. Deduplication of variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) -7. Use prepy in order to normalize test files. This option is only applicable for happy benchmarking of germline analysis ([prepy](https://github.com/Illumina/hap.py/tree/master)) -8. Split SNVs and indels if the given test VCF contains both. This is only applicable for somatic analysis ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) +7. Left aligning of variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) +8. Use prepy in order to normalize test files. This option is only applicable for happy benchmarking of germline analysis ([prepy](https://github.com/Illumina/hap.py/tree/master)) +9. Split SNVs and indels if the given test VCF contains both. This is only applicable for somatic analysis ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) ### Filtering options: Applying filtering on the process of benchmarking itself might makes it impossible to compare different benchmarking strategies. Therefore, for whom like to compare benchmarking methods this subworkflow aims to provide filtering options for variants. -9. Filtration of contigs ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) -10. Include or exclude SNVs and INDELs ([bcftools filter](https://samtools.github.io/bcftools/bcftools.html#filter)) -11. Size and quality filtering for SVs ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) +10. Filtration of contigs ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view)) +11. Include or exclude SNVs and INDELs ([bcftools filter](https://samtools.github.io/bcftools/bcftools.html#filter)) +12. Size and quality filtering for SVs ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) ### Liftover of vcfs: -This sub-workflow provides option to convert genome coordinates of truth VCF and high confidence BED file to a new assembly. Golden standard truth files are build upon specific reference genomes which makes the necessity of lifting over depending on the test VCF in query. Lifting over one or more test vcfs is also possible. +This sub-workflow provides option to convert genome coordinates of truth VCF and test VCFs and high confidence BED file to a new assembly. Golden standard truth files are build upon specific reference genomes which makes the necessity of lifting over depending on the test VCF in query. Lifting over one or more test VCFs is also possible. -12. Create sequence dictionary for the reference ([picard CreateSequenceDictionary](https://gatk.broadinstitute.org/hc/en-us/articles/360037068312-CreateSequenceDictionary-Picard)). This file can be saved and reused. -13. Lifting over truth variants ([picard LiftoverVcf](https://gatk.broadinstitute.org/hc/en-us/articles/360037060932-LiftoverVcf-Picard)) -14. Lifting over high confidence coordinates ([UCSC liftover](http://hgdownload.cse.ucsc.edu/admin/exe)) +13. Create sequence dictionary for the reference ([picard CreateSequenceDictionary](https://gatk.broadinstitute.org/hc/en-us/articles/360037068312-CreateSequenceDictionary-Picard)). This file can be saved and reused. +14. Lifting over VCFs ([picard LiftoverVcf](https://gatk.broadinstitute.org/hc/en-us/articles/360037060932-LiftoverVcf-Picard)) +15. Lifting over high confidence coordinates ([UCSC liftover](http://hgdownload.cse.ucsc.edu/admin/exe)) ### Statistical inference of input test and truth variants: This step provides insights into the distribution of variants before benchmarking. -15. Get statistics of SNVs, INDELs and complex variants ([bcftools stats](https://samtools.github.io/bcftools/bcftools.html#stats)) -16. Get statistics of SVs by type ([SURVIVOR stats](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) +16. Get statistics of SNVs, INDELs and complex variants ([bcftools stats](https://samtools.github.io/bcftools/bcftools.html#stats)) +17. Get statistics of SVs by type ([SURVIVOR stats](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) ### Benchmarking of variants: @@ -71,34 +72,35 @@ Actual benchmarking of variants are split between SVs and small variants: Available methods for SVs: -17. Germline and somatic variant benchmarking using Truvari ([truvari bench](https://github.com/acenglish/truvari/wiki/bench)) -18. Germline and somatic variant benchmarking using SVanalyzer ([svanalyzer benchmark](https://github.com/nhansen/SVanalyzer/blob/master/docs/svbenchmark.rst)) +18. Germline and somatic variant benchmarking using Truvari ([truvari bench](https://github.com/acenglish/truvari/wiki/bench)) +19. Germline and somatic variant benchmarking using SVanalyzer ([svanalyzer benchmark](https://github.com/nhansen/SVanalyzer/blob/master/docs/svbenchmark.rst)) Available methods for CNVs: -19. Germline and somatic variant benchmarking using Wittyer ([witty.er](https://github.com/Illumina/witty.er/tree/master)) +20. Germline and somatic variant benchmarking using Wittyer ([witty.er](https://github.com/Illumina/witty.er/tree/master)) Available methods for SNVs and INDELs: -20. Germline variant benchmarking using RTG-tools ([rtg vcfeval](https://realtimegenomics.com/products/rtg-tools)) -21. Germline variant benchmarking using Happy tools ([hap.py](https://github.com/Illumina/hap.py/blob/master/doc/happy.md)) -22. Somatic variant benchmarking using Sompy ([som.py](https://github.com/Illumina/hap.py/tree/master?tab=readme-ov-file#sompy)) +21. Germline variant benchmarking using RTG-tools ([rtg vcfeval](https://realtimegenomics.com/products/rtg-tools)) +22. Germline variant benchmarking using Happy tools ([hap.py](https://github.com/Illumina/hap.py/blob/master/doc/happy.md)) +23. Somatic variant benchmarking using Sompy ([som.py](https://github.com/Illumina/hap.py/tree/master?tab=readme-ov-file#sompy)) ### Comparison of benchmarking results per TP, FP and FN files It is essential to compare benchmarking results in order to infer uniquely or commonly seen TPs, FPs and FNs. -23. Merging TP, FP and FN results for happy, rtgtools and sompy ([bcftools merge](https://samtools.github.io/bcftools/bcftools.html#merge)) -24. Merging TP, FP and FN results for Truvari and SVanalyzer ([SURVIVOR merge](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) -25. Conversion of VCF files to CSV to infer common and unique variants per caller (python script) +24. Merging TP, FP and FN results for happy, rtgtools and sompy ([bcftools merge](https://samtools.github.io/bcftools/bcftools.html#merge)) +25. Merging TP, FP and FN results for Truvari and SVanalyzer ([SURVIVOR merge](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) +26. Conversion of VCF files to CSV to infer common and unique variants per caller (python script) ### Reporting of benchmark results The generation of comprehensive report that consolidates all benchmarking results. -26. Merging summary statistics per benchmarking tool (python script) -27. Plotting benchmark metrics per benchmarking tool (R script) -28. Create visual HTML report for the integration of NCBENCH ([datavzrd](https://datavzrd.github.io/docs/index.html)) +27. Merging summary statistics per benchmarking tool (python script) +28. Plotting benchmark metrics per benchmarking tool (R script) +29. Create visual HTML report for the integration of NCBENCH ([datavzrd](https://datavzrd.github.io/docs/index.html)) +30. Apply MultiQC to visualize results @@ -121,7 +123,7 @@ test3,test3.vcf.gz,cnvkit Each row represents a vcf file (test-query file). For each vcf file and variant calling method (caller) have to be defined. -User has to define or provide truth vcf in config files. There are readily available vcf files for benchmarking from Genome in a bottle and SEQC2 studies which can be used readily. Please find detailed information about truth files [here](https://nf-co.re/variantbenchmarking/truth) +User _has to provide truth vcf in config files_. There are readily available vcf files for benchmarking from Genome in a bottle and SEQC2 studies which can be used readily. Please find detailed information about truth files [here](https://nf-co.re/variantbenchmarking/truth) For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters). @@ -133,8 +135,9 @@ nextflow run nf-core/variantbenchmarking \ --input samplesheet.csv \ --outdir \ --genome GRCh37 \ - --sample HG002 - --analysis germline + --analysis germline \ + --truth_id HG002 \ + --truth_vcf truth.vcf.gz ``` > [!WARNING] diff --git a/conf/modules.config b/conf/modules.config index f85cce6..9c9888d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -25,48 +25,115 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } - // standardization and normalization tools - withName: "BCFTOOLS_NORM" { - ext.prefix = { vcf.baseName - ".vcf" + ".norm"} - ext.args = {"--output-type z -c w --write-index=tbi" } + // subsample_vcf test + + withName: BCFTOOLS_SORT { + ext.prefix = { vcf.baseName - ".vcf" + ".sort"} + ext.args = {"--output-type z --write-index=tbi" } + publishDir = [ + enabled: false + ] } - withName: "BCFTOOLS_SPLIT_MULTI" { - ext.prefix = { vcf.baseName - ".vcf" + ".split"} - ext.args = {"--output-type z -m-any -c w --write-index=tbi" } + withName: BCFTOOLS_VIEW_SUBSAMPLE { + ext.prefix = { vcf.baseName - ".vcf" + ".subsample" } + ext.args = {"--output-type z -s ${meta.subsample}" } + publishDir = [ + enabled: false + ] } - withName: "VARIANT_EXTRACTOR" { - ext.prefix = { input.baseName - ".vcf" } + withName: BCFTOOLS_VIEW_FILTERMISSING { + ext.prefix = { vcf.baseName - ".vcf" + ".filtermissing" } + ext.args = {"--output-type z -e 'AC=0'" } publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, - pattern: "*{.vcf.gz}", - mode: params.publish_dir_mode + enabled: false + ] + } + + // sv_vcf_conversions + + withName: VARIANT_EXTRACTOR { + ext.prefix = { input.baseName - ".vcf" + ".variantextract" } + publishDir = [ + enabled: false ] } + withName: SVYNC { - ext.prefix = {"${meta.id}.${meta.caller}.svync"} + ext.prefix = {vcf.baseName - ".vcf" + ".svync"} publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, - pattern: "*{.vcf.gz,vcf.gz.tbi}", + path: { "${params.outdir}/test" }, + enabled: false + ] + } + + withName: BGZIP_TABIX { + publishDir = [ + enabled: false + ] + } + //// prepare_vcfs //// + + // liftover_vcfs + + withName: PICARD_CREATESEQUENCEDICTIONARY { + publishDir = [ + path: {"${params.outdir}/references/dictionary"}, + pattern: "*{.dict}", mode: params.publish_dir_mode ] } - withName: "BCFTOOLS_DEDUP" { - ext.prefix = { vcf.baseName - ".vcf" + ".dedup"} - ext.args = {"--output-type z --rm-du exact -c w" } + + withName: PICARD_LIFTOVERVCF { + ext.prefix = {input_vcf.baseName - ".vcf"} + ext.args = {"--WARN_ON_MISSING_CONTIG true"} + publishDir = [ + enabled: false + ] } - withName: "BCFTOOLS_SORT" { - ext.prefix = { vcf.baseName - ".vcf" + ".sort"} - ext.args = {"--output-type z --write-index=tbi" } + withName: BCFTOOLS_RENAME_CHR { + ext.prefix = {input.baseName - ".vcf" + ".renamechr"} + ext.args = {"--output-type z"} publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, + path: {params.liftover == "truth"? "${params.outdir}/${params.variant_type}/${params.truth_id}/preprocess/liftover" : "${params.outdir}/${params.variant_type}/${meta.id}/preporcess/liftover"}, pattern: "*{.vcf.gz}", mode: params.publish_dir_mode ] } - withName: "BCFTOOLS_REHEADER" { + withName: UCSC_LIFTOVER { + ext.prefix = {bed.baseName - ".bed"} + publishDir = [ + enabled: false + ] + } + withName: SORT_BED { + ext.prefix = {bed.baseName - ".bed" + "sort"} + publishDir = [ + enabled: false + ] + } + withName: BEDTOOLS_MERGE { + ext.prefix = {bed.toString() - ".bed" + ".merged" } + publishDir = [ + path: {params.liftover == "truth"? "${params.outdir}/${params.variant_type}/${params.truth_id}/preprocess/liftover" : "${params.outdir}/${params.variant_type}/${meta.id}/preporcess/liftover"}, + pattern: "*{.bed}", + mode: params.publish_dir_mode + ] + } + withName: REFORMAT_HEADER { + ext.prefix ={["${meta.id}", + (meta.tag != null) ? ".${meta.tag}" : '' + ].join('').trim() + } + publishDir = [ + enabled: false + ] + + } + + withName: "BCFTOOLS_REHEADER*" { beforeScript = {[ "echo ${meta.id}", (meta.caller != null )? ".${meta.caller}" : "", @@ -74,31 +141,51 @@ process { ].join('').trim()} ext.args = { "--samples ${meta.id}.txt" } ext.args2 = {"--output-type z --write-index=tbi" } - ext.prefix = { vcf.baseName - ".vcf" + ".rh"} + ext.prefix = { vcf.baseName - ".vcf" + ".reheader"} + publishDir = [ + enabled: false + ] } - // splitting tools - withName: BCFTOOLS_VIEW_SUBSAMPLE { - ext.prefix = { vcf.baseName - ".vcf" + ".subsample" } - ext.args = {"--output-type z -s ${meta.subsample}" } + + // filtering contigs + withName: BCFTOOLS_VIEW_CONTIGS { + ext.prefix = { vcf.baseName - ".vcf.gz" + ".filtercontigs" } + ext.args = {[ + "--output-type z --write-index=tbi", + (params.genome.contains("38"))? "-r chr1,chr2,chr3,chr4,chr5,chr6,chr7,chr8,chr9,chr10,chr11,chr12,chr13,chr14,chr15,chr16,chr17,chr18,chr19,chr20,chr21,chr22,chrX,chrY" : "-r 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,X,Y" + ].join(' ').trim() } + publishDir = [ + enabled: false + ] } - withName: BCFTOOLS_VIEW_SNV { - ext.prefix = { vcf.baseName - ".vcf" + ".snv" } - ext.args = {"--output-type v --types snps" } + + // bcftools normalize + withName: BCFTOOLS_NORM { + ext.prefix = { vcf.baseName - ".vcf" + ".norm"} + ext.args = {"--output-type z -c w --write-index=tbi" } publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, - pattern: "*{.vcf}", - mode: params.publish_dir_mode + enabled: false ] } - withName: BCFTOOLS_VIEW_INDEL { - ext.prefix = { vcf.baseName - ".vcf" + ".indel" } - ext.args = {"--output-type v --types indels" } + + //bcftools split multi allelics + withName: BCFTOOLS_SPLIT_MULTI { + ext.prefix = { vcf.baseName - ".vcf" + ".split"} + ext.args = {"--output-type z -m-any -c w --write-index=tbi" } publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, - pattern: "*{.vcf}", - mode: params.publish_dir_mode + enabled: false + ] + } + + // bcftools deduplicate variants + withName: BCFTOOLS_DEDUP { + ext.prefix = { vcf.baseName - ".vcf" + ".dedup"} + ext.args = {"--output-type z --rm-du exact -c w" } + publishDir = [ + enabled: false ] } + // filtering tools withName: BCFTOOLS_FILTER { ext.prefix = { vcf.baseName - ".vcf" + ".filter"} @@ -108,39 +195,51 @@ process { (params.exclude_expression != null )? "--exclude '$params.exclude_expression'" : "" ].join(' ').trim() } publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess/"}, - pattern: "*{.vcf}", - mode: params.publish_dir_mode + enabled: false + ] + } + + withName: TABIX_TABIX { + publishDir = [ + enabled: false ] } + withName: SURVIVOR_FILTER { ext.prefix = { vcf_file.baseName - ".vcf" + ".filter"} publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, - pattern: "*{.vcf}", - mode: params.publish_dir_mode + enabled: false ] } - withName: BCFTOOLS_VIEW_FILTERMISSING { - ext.prefix = { vcf.baseName - ".vcf" + ".filtermiss" } - ext.args = {"--output-type z -e 'AC=0'" } + + // split_small_variants_test + + withName: BCFTOOLS_VIEW_SNV { + ext.prefix = { vcf.baseName - ".vcf" + ".snv" } + ext.args = {"--output-type v --types snps" } publishDir = [ - path: { "${params.outdir}/test" }, enabled: false ] } - withName: BCFTOOLS_VIEW_CONTIGS { - ext.prefix = { vcf.baseName - ".vcf.gz" + ".nocontigs" } - ext.args = {[ - "--output-type z --write-index=tbi", - (params.genome.contains("38"))? "-r chr1,chr2,chr3,chr4,chr5,chr6,chr7,chr8,chr9,chr10,chr11,chr12,chr13,chr14,chr15,chr16,chr17,chr18,chr19,chr20,chr21,chr22,chrX,chrY" : "-r 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,X,Y" - ].join(' ').trim() } + + withName: BCFTOOLS_VIEW_INDEL { + ext.prefix = { vcf.baseName - ".vcf" + ".indel" } + ext.args = {"--output-type v --types indels" } publishDir = [ - path: { "${params.outdir}/test" }, enabled: false ] } - // Variant stats + + withName: 'PUBLISH_PROCESSED_VCF' { + publishDir = [ + path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, + pattern: "*{.vcf.gz,vcf.gz.tbi}", + mode: params.publish_dir_mode + ] + } + + // report_vcf_statistics + withName: SURVIVOR_STATS { ext.prefix ={["${meta.id}", (meta.caller != null) ? ".${meta.caller}_mqc" : '_mqc' @@ -152,6 +251,7 @@ process { mode: params.publish_dir_mode ] } + withName: BCFTOOLS_STATS { ext.prefix ={["${meta.id}", (meta.caller != null) ? ".${meta.caller}" : '' @@ -163,7 +263,9 @@ process { mode: params.publish_dir_mode ] } - // benchmark tools + + //// benchmarking // + withName: "RTGTOOLS_FORMAT" { publishDir = [ path: {"${params.outdir}/references/rtgtools"}, @@ -171,6 +273,7 @@ process { mode: params.publish_dir_mode ] } + withName: "RTGTOOLS_VCFEVAL" { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} //ext.args = {"--ref-overlap --all-record --output-mode ga4gh"} @@ -180,6 +283,7 @@ process { mode: params.publish_dir_mode ] } + withName: "HAPPY_HAPPY" { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} //ext.args = {""} @@ -189,6 +293,7 @@ process { mode: params.publish_dir_mode ] } + withName: "HAPPY_SOMPY" { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = { meta.caller.contains("strelka") || meta.caller.contains("varscan") || meta.caller.contains("pisces") ? "--feature-table hcc.${meta.caller}.${params.variant_type} --bin-afs" : "--feature-table generic" } @@ -198,15 +303,15 @@ process { mode: params.publish_dir_mode ] } + withName: "HAPPY_PREPY" { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}.prepy"} ext.args = {"--fixchr --filter-nonref --bcftools-norm"} publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/preprocess"}, - pattern: "*{vcf.gz}", - mode: params.publish_dir_mode + enabled: false ] } + withName: "TRUVARI_BENCH" { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = {[ @@ -225,6 +330,7 @@ process { mode: params.publish_dir_mode ] } + withName: SVANALYZER_SVBENCHMARK { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = {[ @@ -239,6 +345,7 @@ process { mode: params.publish_dir_mode ] } + withName: WITTYER { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} ext.args = {[ @@ -255,6 +362,19 @@ process { mode: params.publish_dir_mode ] } + + withName: "TABIX_BGZIP*"{ + ext.prefix = {input.toString() - ".vcf.gz"} + publishDir = [ + enabled: false + ] + } + withName: "TABIX_BGZIPTABIX*"{ + ext.prefix = { input.baseName - ".vcf" + ".bgzip"} + publishDir = [ + enabled: false + ] + } withName: BAMSURGEON_EVALUATOR { ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} publishDir = [ @@ -263,7 +383,9 @@ process { mode: params.publish_dir_mode ] } - // summary reports + + // report_benchmark_statistics + withName: MERGE_REPORTS { ext.prefix = {"${meta.benchmark_tool}"} publishDir = [ @@ -272,6 +394,7 @@ process { mode: params.publish_dir_mode ] } + withName: PLOTS { ext.prefix = {"${meta.benchmark_tool}_mqc"} publishDir = [ @@ -280,6 +403,13 @@ process { mode: params.publish_dir_mode ] } + + withName: CREATE_DATAVZRD_INPUT { + publishDir = [ + enabled: false + ] + } + withName: DATAVZRD { ext.prefix = {"${meta.id}"} publishDir = [ @@ -288,17 +418,31 @@ process { mode: params.publish_dir_mode ] } - // compare vcf results - withName: "TABIX_BGZIP*"{ + + // compare_benchmark_results + + withName: TABIX_BGZIP_UNZIP{ ext.prefix = {input.toString() - ".vcf.gz"} + publishDir = [ + enabled: false + ] } + withName: SURVIVOR_MERGE { ext.prefix = {"${meta.id}.${meta.tag}"} + publishDir = [ + enabled: false + ] } + withName: BCFTOOLS_MERGE { ext.prefix = {"${meta.id}.${meta.tag}"} ext.args = {"--output-type v --force-samples --force-single"} + publishDir = [ + enabled: false + ] } + withName: VCF_TO_CSV { ext.prefix = {"${meta.id}.${meta.tag}"} publishDir = [ @@ -307,56 +451,5 @@ process { mode: params.publish_dir_mode ] } - withName: REFORMAT_HEADER { - ext.prefix ={["${meta.id}", - (meta.tag != null) ? ".${meta.tag}" : '' - ].join('').trim() - } - } - // liftOver - withName: PICARD_CREATESEQUENCEDICTIONARY { - publishDir = [ - path: {"${params.outdir}/references/dictionary"}, - pattern: "*{.dict}", - mode: params.publish_dir_mode - ] - } - withName: PICARD_LIFTOVERVCF { - ext.prefix = {"${meta.id}"} - ext.args = {"--WARN_ON_MISSING_CONTIG true"} - } - withName: BCFTOOLS_RENAME_CHR { - ext.prefix = {"${meta.id}.renamechr"} - ext.args = {"--output-type z"} - publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${params.truth_id}/liftover"}, - pattern: "*{.vcf.gz}", - mode: params.publish_dir_mode - ] - } - withName: UCSC_LIFTOVER { - ext.prefix = {"${meta.id}.liftover"} - } - withName: SORT_BED { - ext.prefix = {"${meta.id}.sort"} - } - withName: BEDTOOLS_MERGE { - ext.prefix = {bed.toString() - ".bed" + ".merged" } - publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${params.truth_id}/liftover"}, - pattern: "*{.bed}", - mode: params.publish_dir_mode - ] - } -} -// -// Don't publish results for these processes -// -process { - withName: 'TABIX_TABIX|TABIX_BGZIP|TABIX_BGZIPTABIX|BGZIP_TABIX|SURVIVOR_MERGE|BCFTOOLS_MERGE|REFORMAT_HEADER|BCFTOOLS_SPLIT_MULTI|BCFTOOLS_DEDUP|BCFTOOLS_REHEADER|SORT_BED|UCSC_LIFTOVER|PICARD_LIFTOVERVCF|BCFTOOLS_VIEW_SUBSAMPLE|CREATE_DATAVZRD_INPUT' { - publishDir = [ - path: { "${params.outdir}/test" }, - enabled: false - ] - } + } diff --git a/conf/test.config b/conf/test.config index 871b85d..4715b0e 100644 --- a/conf/test.config +++ b/conf/test.config @@ -33,8 +33,8 @@ params { analysis = 'germline' variant_type = "small" method = 'happy,rtgtools' - preprocess = "normalize,deduplicate,prepy,split_multiallelic,filter_contigs" - include_expression = 'FILTER="."' + preprocess = "normalize,deduplicate,prepy" + // truth information truth_id = "HG002" diff --git a/conf/tests/liftover_truth.config b/conf/tests/liftover_truth.config index de1b854..e16bd88 100644 --- a/conf/tests/liftover_truth.config +++ b/conf/tests/liftover_truth.config @@ -29,7 +29,7 @@ params { truth_id = "HG002" variant_type = "small" method = 'rtgtools,happy' - preprocess = "split_multiallelic,deduplicate,filter_contigs" + preprocess = "split_multiallelic,deduplicate,filter_contigs,normalize" truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" diff --git a/docs/output.md b/docs/output.md index 8d8771c..40fe887 100644 --- a/docs/output.md +++ b/docs/output.md @@ -33,7 +33,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
    Output files -- `preprocesses/` +- `mpreprocesses/` - `*.vcf.gz`: The standardized and normalized VCF files
    @@ -47,7 +47,7 @@ Outputs from standardization, normalization and filtration processes saved. When - ## `liftover/` -- `liftover/` +- `preporocesses/liftover/` - `*.vcf.gz`: Lifted over variants - `*.bed`: Lifted over regions diff --git a/docs/usage.md b/docs/usage.md index 7471a45..9c7e7d5 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -73,12 +73,24 @@ Consistent formatting and alignment of variants in test and truth VCF files for - `homogenize`: makes use of [variant-extractor](https://github.com/EUCANCan/variant-extractor) - `svync`: makes use of [svync](https://github.com/nvnieuwk/svync) -- `--preprocesses`: The preprocessing steps to perform on the input files. Should be a comma-separated list of one or more of the following options: `normalization,deduplication,prepy,filter_contigs` - - `normalization`: Splits multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) - - `deduplication`: Deduplicates variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) +- `--preprocesses`: The preprocessing steps to perform on the input files. Should be a comma-separated list of one or more of the following options: `split_multiallelic,normalize,deduplicate,prepy,filter_contigs` + - `split_multiallelic`: Splits multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) + - `normalize`: Left aligns variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) + - `deduplicate`: Deduplicates variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm)) - `prepy`: Uses prepy in order to normalize test files. This option is only applicable for happy benchmarking of germline analysis ([prepy](https://github.com/Illumina/hap.py/tree/master)) - `filter_contigs`: Filter out extra contigs. It is common for truth files not to include extra contigs. +Filtration of tst variants are controlled through the following parameters: + +- `exclude_expression`: Use ([bcftools expressions](https://samtools.github.io/bcftools/bcftools.html#expressions) to exclude variants) +- `include_expression`: Use ([bcftools expressions](https://samtools.github.io/bcftools/bcftools.html#expressions) to include variants) +- `min_sv_size`: Minimum SV size of variants to benchmark. Uses ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) +- `max_sv_size`: Maximum SV size of variants to benchmark. Uses ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) +- `min_allele_freq`: Minimum Alele Frequency of variants to benchmark for SVs. Uses ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) +- `min_num_reads`: Minimum number of read supporting variants to benchmark for SVs. Uses ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki)) + +_tip_: One can use _exclude_expression_ or _include_expression_ to limit indel or SV variant size as well. + ## Using multi-sample vcf inputs If the input test vcf contains more than one sample, then user has to define which sample name to use. `subsample` will added to the samplesheet as an additional column as follows: diff --git a/main.nf b/main.nf index 2e63537..d84ea2b 100644 --- a/main.nf +++ b/main.nf @@ -35,7 +35,7 @@ include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_vari ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// WORKFLOW: Run main nf-core/sarek analysis pipeline +// WORKFLOW: Run main nf-core/variantbenchmarking analysis pipeline workflow NFCORE_VARIANTBENCHMARKING { take: diff --git a/modules/local/custom/publish_processed_vcf/environment.yml b/modules/local/custom/publish_processed_vcf/environment.yml new file mode 100644 index 0000000..203c32c --- /dev/null +++ b/modules/local/custom/publish_processed_vcf/environment.yml @@ -0,0 +1,8 @@ +name: publish_processed_vcf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::tabix=1.11 + - bioconda::htslib=1.19.1 diff --git a/modules/local/custom/publish_processed_vcf/main.nf b/modules/local/custom/publish_processed_vcf/main.nf new file mode 100644 index 0000000..d13313c --- /dev/null +++ b/modules/local/custom/publish_processed_vcf/main.nf @@ -0,0 +1,35 @@ +process PUBLISH_PROCESSED_VCF { + tag "$meta.id" + label 'process_single' + + conda "" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : + 'biocontainers/tabix:1.11--hdfd78af_0' }" + + input: + tuple val(meta), path(vcf), path(index) + + output: + tuple val(meta),path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: gz_tbi + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + cp $vcf ${prefix}.vcf.gz + cp $index ${prefix}.vcf.gz.tbi + + """ + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + + """ +} diff --git a/modules/nf-core/bcftools/query/environment.yml b/modules/nf-core/bcftools/query/environment.yml deleted file mode 100644 index d8c4f4e..0000000 --- a/modules/nf-core/bcftools/query/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: bcftools_query -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::bcftools=1.20 diff --git a/modules/nf-core/bcftools/query/main.nf b/modules/nf-core/bcftools/query/main.nf deleted file mode 100644 index 58019f4..0000000 --- a/modules/nf-core/bcftools/query/main.nf +++ /dev/null @@ -1,56 +0,0 @@ -process BCFTOOLS_QUERY { - tag "$meta.id" - label 'process_single' - - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/bcftools:1.20--h8b25389_0': - 'biocontainers/bcftools:1.20--h8b25389_0' }" - - input: - tuple val(meta), path(vcf), path(tbi) - path regions - path targets - path samples - - output: - tuple val(meta), path("*.${suffix}"), emit: output - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - suffix = task.ext.suffix ?: "txt" - def regions_file = regions ? "--regions-file ${regions}" : "" - def targets_file = targets ? "--targets-file ${targets}" : "" - def samples_file = samples ? "--samples-file ${samples}" : "" - """ - bcftools query \\ - $regions_file \\ - $targets_file \\ - $samples_file \\ - $args \\ - $vcf \\ - > ${prefix}.${suffix} - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ - - stub: - def prefix = task.ext.prefix ?: "${meta.id}" - suffix = task.ext.suffix ?: "txt" - """ - touch ${prefix}.${suffix} \\ - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bcftools: \$(bcftools --version 2>&1 | head -n1 | sed 's/^.*bcftools //; s/ .*\$//') - END_VERSIONS - """ -} diff --git a/modules/nf-core/bcftools/query/meta.yml b/modules/nf-core/bcftools/query/meta.yml deleted file mode 100644 index 303ef61..0000000 --- a/modules/nf-core/bcftools/query/meta.yml +++ /dev/null @@ -1,63 +0,0 @@ -name: bcftools_query -description: Extracts fields from VCF or BCF files and outputs them in user-defined format. -keywords: - - query - - variant calling - - bcftools - - VCF -tools: - - query: - description: | - Extracts fields from VCF or BCF files and outputs them in user-defined format. - homepage: http://samtools.github.io/bcftools/bcftools.html - documentation: http://www.htslib.org/doc/bcftools.html - doi: 10.1093/bioinformatics/btp352 - licence: ["MIT"] -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: | - The vcf file to be qeuried. - pattern: "*.{vcf.gz, vcf}" - - tbi: - type: file - description: | - The tab index for the VCF file to be inspected. - pattern: "*.tbi" - - regions: - type: file - description: | - Optionally, restrict the operation to regions listed in this file. - - targets: - type: file - description: | - Optionally, restrict the operation to regions listed in this file (doesn't rely upon index files) - - samples: - type: file - description: | - Optional, file of sample names to be included or excluded. - e.g. 'file.tsv' -output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - output: - type: file - description: BCFTools query output file - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@abhi18av" - - "@drpatelh" -maintainers: - - "@abhi18av" - - "@drpatelh" diff --git a/modules/nf-core/bcftools/query/tests/main.nf.test b/modules/nf-core/bcftools/query/tests/main.nf.test deleted file mode 100644 index e9ea5a9..0000000 --- a/modules/nf-core/bcftools/query/tests/main.nf.test +++ /dev/null @@ -1,101 +0,0 @@ -nextflow_process { - - name "Test Process BCFTOOLS_QUERY" - script "../main.nf" - process "BCFTOOLS_QUERY" - - tag "modules" - tag "modules_nfcore" - tag "bcftools" - tag "bcftools/query" - - config "./nextflow.config" - - test("sarscov2 - [vcf, tbi], [], [], []") { - - when { - process { - """ - input[0] = [ - [ id:'out' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) - ] - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.output, - process.out.versions - ).match() } - ) - } - - } - - test("sarscov2 - [vcf, tbi], vcf, tsv, []") { - - when { - process { - """ - input[0] = [ - [ id:'out' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) - ] - input[1] = file(params.test_data['sarscov2']['illumina']['test3_vcf_gz'], checkIfExists: true) - input[2] = file(params.test_data['sarscov2']['illumina']['test2_vcf_targets_tsv_gz'], checkIfExists: true) - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.output, - process.out.versions - ).match() } - ) - } - - } - - test("sarscov2 - [vcf, tbi], [], [], [] - stub") { - - when { - process { - """ - input[0] = [ - [ id:'out' ], // meta map - file(params.test_data['sarscov2']['illumina']['test_vcf_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_vcf_gz_tbi'], checkIfExists: true) - ] - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - file(process.out.output[0][1]).name, - process.out.versions - ).match() } - ) - } - - } - -} diff --git a/modules/nf-core/bcftools/query/tests/main.nf.test.snap b/modules/nf-core/bcftools/query/tests/main.nf.test.snap deleted file mode 100644 index 3ead1f2..0000000 --- a/modules/nf-core/bcftools/query/tests/main.nf.test.snap +++ /dev/null @@ -1,55 +0,0 @@ -{ - "sarscov2 - [vcf, tbi], vcf, tsv, []": { - "content": [ - [ - [ - { - "id": "out" - }, - "out.txt:md5,75a6bd0084e2e1838cf7baba11b99d19" - ] - ], - [ - "versions.yml:md5,3d93ea9cd5d314743254618b49e4bd16" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-05-31T15:15:44.916249758" - }, - "sarscov2 - [vcf, tbi], [], [], [] - stub": { - "content": [ - "out.txt", - [ - "versions.yml:md5,3d93ea9cd5d314743254618b49e4bd16" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-05-31T15:15:49.932359271" - }, - "sarscov2 - [vcf, tbi], [], [], []": { - "content": [ - [ - [ - { - "id": "out" - }, - "out.txt:md5,87a2ab194e1ee3219b44e58429ec3307" - ] - ], - [ - "versions.yml:md5,3d93ea9cd5d314743254618b49e4bd16" - ] - ], - "meta": { - "nf-test": "0.8.4", - "nextflow": "23.10.1" - }, - "timestamp": "2024-05-31T15:15:39.930697926" - } -} \ No newline at end of file diff --git a/modules/nf-core/bcftools/query/tests/nextflow.config b/modules/nf-core/bcftools/query/tests/nextflow.config deleted file mode 100644 index da81c2a..0000000 --- a/modules/nf-core/bcftools/query/tests/nextflow.config +++ /dev/null @@ -1,3 +0,0 @@ -process { - ext.args = "-f '%CHROM %POS %REF %ALT[%SAMPLE=%GT]'" -} \ No newline at end of file diff --git a/modules/nf-core/bcftools/query/tests/tags.yml b/modules/nf-core/bcftools/query/tests/tags.yml deleted file mode 100644 index fb9455c..0000000 --- a/modules/nf-core/bcftools/query/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -bcftools/query: - - "modules/nf-core/bcftools/query/**" diff --git a/nextflow.config b/nextflow.config index f1e969b..2badde6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -32,7 +32,7 @@ params { sv_standardization = "" // Filtering parameters - min_sv_size = 30 + min_sv_size = 0 max_sv_size = -1 min_allele_freq = -1 min_num_reads = -1 diff --git a/subworkflows/local/compare_benchmark_results.nf b/subworkflows/local/compare_benchmark_results.nf index 22fed95..9aa790e 100644 --- a/subworkflows/local/compare_benchmark_results.nf +++ b/subworkflows/local/compare_benchmark_results.nf @@ -6,8 +6,8 @@ include { SURVIVOR_MERGE } from '../../modules/nf-core/survivor/merge' include { BCFTOOLS_MERGE } from '../../modules/nf-core/bcftools/merge' include { VCF_TO_CSV } from '../../modules/local/vcf_to_csv' -include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' include { REFORMAT_HEADER } from '../../modules/local/custom/reformat_header' +include { TABIX_BGZIP as TABIX_BGZIP_UNZIP } from '../../modules/nf-core/tabix/bgzip' workflow COMPARE_BENCHMARK_RESULTS { take: @@ -40,12 +40,12 @@ workflow COMPARE_BENCHMARK_RESULTS { else{ // SV part // unzip vcfs - TABIX_BGZIP( + TABIX_BGZIP_UNZIP( evaluations ) - versions = versions.mix(TABIX_BGZIP.out.versions.first()) + versions = versions.mix(TABIX_BGZIP_UNZIP.out.versions.first()) - TABIX_BGZIP.out.output + TABIX_BGZIP_UNZIP.out.output .groupTuple() .set{vcf_ch} diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test.nf index 01a169b..cdd9b9c 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test.nf @@ -2,15 +2,16 @@ // PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS // -include { BCFTOOLS_REHEADER } from '../../modules/nf-core/bcftools/reheader' include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' include { VCF_VARIANT_FILTERING } from '../local/vcf_variant_filtering' include { SPLIT_SMALL_VARIANTS_TEST } from '../local/split_small_variants_test' include { LIFTOVER_VCFS } from '../local/liftover_vcfs' include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' include { FIX_VCF_PREFIX } from '../../modules/local/custom/fix_vcf_prefix' -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_CONTIGS } from '../../modules/nf-core/bcftools/view' -include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../modules/nf-core/bcftools/norm' +include { PUBLISH_PROCESSED_VCF } from '../../modules/local/custom/publish_processed_vcf' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_CONTIGS } from '../../modules/nf-core/bcftools/view' +include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../modules/nf-core/bcftools/norm' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_QUERY} from '../../modules/nf-core/bcftools/reheader' workflow PREPARE_VCFS_TEST { @@ -58,7 +59,7 @@ workflow PREPARE_VCFS_TEST { vcf_ch = Channel.empty() - // fic vcf chromosome prefix according to reference genome + // fix vcf chromosome prefix according to reference genome FIX_VCF_PREFIX( fix.prefix, rename_chr @@ -69,16 +70,16 @@ workflow PREPARE_VCFS_TEST { // Add "query" to test sample // rename sample name - BCFTOOLS_REHEADER( + BCFTOOLS_REHEADER_QUERY( vcf_ch.map{ meta, file -> [ meta, file, [], [] ] }, fai ) - versions = versions.mix(BCFTOOLS_REHEADER.out.versions.first()) + versions = versions.mix(BCFTOOLS_REHEADER_QUERY.out.versions.first()) - BCFTOOLS_REHEADER.out.vcf.join(BCFTOOLS_REHEADER.out.index) + BCFTOOLS_REHEADER_QUERY.out.vcf.join(BCFTOOLS_REHEADER_QUERY.out.index) .set{vcf_ch} if (params.preprocess.contains("filter_contigs")){ @@ -131,7 +132,7 @@ workflow PREPARE_VCFS_TEST { if (params.preprocess.contains("normalize")){ - // Turn on left alignment and m\normalization + // Turn on left alignment and normalization BCFTOOLS_NORM( vcf_ch, fasta @@ -155,6 +156,10 @@ workflow PREPARE_VCFS_TEST { } + PUBLISH_PROCESSED_VCF( + vcf_ch + ) + emit: vcf_ch // channel: [val(meta), vcf.gz, tbi] versions // channel: [versions.yml] diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth.nf index 22ba168..bbf3834 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth.nf @@ -3,11 +3,12 @@ // -include { BCFTOOLS_REHEADER } from '../../modules/nf-core/bcftools/reheader' include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' include { LIFTOVER_VCFS } from '../local/liftover_vcfs' include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' -include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../modules/nf-core/bcftools/norm' +include { PUBLISH_PROCESSED_VCF } from '../../modules/local/custom/publish_processed_vcf' +include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../modules/nf-core/bcftools/norm' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TRUTH} from '../../modules/nf-core/bcftools/reheader' workflow PREPARE_VCFS_TRUTH { @@ -42,15 +43,15 @@ workflow PREPARE_VCFS_TRUTH { // Reheader sample name for truth file - using meta.caller // rename sample name - BCFTOOLS_REHEADER( + BCFTOOLS_REHEADER_TRUTH( truth_ch.map{ meta, file -> [ meta, file, [], [] ] }, fai ) - versions = versions.mix(BCFTOOLS_REHEADER.out.versions.first()) + versions = versions.mix(BCFTOOLS_REHEADER_TRUTH.out.versions.first()) - BCFTOOLS_REHEADER.out.vcf.join(BCFTOOLS_REHEADER.out.index) + BCFTOOLS_REHEADER_TRUTH.out.vcf.join(BCFTOOLS_REHEADER_TRUTH.out.index) .set{vcf_ch} if (params.preprocess.contains("split_multiallelic")){ @@ -90,6 +91,9 @@ workflow PREPARE_VCFS_TRUTH { .set{vcf_ch} } + PUBLISH_PROCESSED_VCF( + vcf_ch + ) emit: vcf_ch // channel: [val(meta), vcf, tbi] high_conf_ch // channel: [val(meta), bed] diff --git a/subworkflows/local/split_small_variants_test.nf b/subworkflows/local/split_small_variants_test.nf index 00f26fd..d595729 100644 --- a/subworkflows/local/split_small_variants_test.nf +++ b/subworkflows/local/split_small_variants_test.nf @@ -4,8 +4,8 @@ include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_SNV } from '../../modules/nf-core/bcftools/view' include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_INDEL } from '../../modules/nf-core/bcftools/view' -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_1 } from '../../modules/nf-core/tabix/bgziptabix' -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_2 } from '../../modules/nf-core/tabix/bgziptabix' +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_SNV } from '../../modules/nf-core/tabix/bgziptabix' +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_INDEL } from '../../modules/nf-core/tabix/bgziptabix' workflow SPLIT_SMALL_VARIANTS_TEST { take: @@ -25,12 +25,12 @@ workflow SPLIT_SMALL_VARIANTS_TEST { ) versions = versions.mix(BCFTOOLS_VIEW_SNV.out.versions.first()) - TABIX_BGZIPTABIX_1( + TABIX_BGZIPTABIX_SNV( BCFTOOLS_VIEW_SNV.out.vcf ) - versions = versions.mix(TABIX_BGZIPTABIX_1.out.versions.first()) + versions = versions.mix(TABIX_BGZIPTABIX_SNV.out.versions.first()) - TABIX_BGZIPTABIX_1.out.gz_tbi + TABIX_BGZIPTABIX_SNV.out.gz_tbi .map { meta, file, index -> tuple(meta + [vartype: "snv"], file, index) } .set{split_snv_vcf} out_vcf_ch = out_vcf_ch.mix(split_snv_vcf) @@ -43,11 +43,11 @@ workflow SPLIT_SMALL_VARIANTS_TEST { ) versions = versions.mix(BCFTOOLS_VIEW_INDEL.out.versions.first()) - TABIX_BGZIPTABIX_2( + TABIX_BGZIPTABIX_INDEL( BCFTOOLS_VIEW_INDEL.out.vcf ) - versions = versions.mix(TABIX_BGZIPTABIX_2.out.versions.first()) - TABIX_BGZIPTABIX_2.out.gz_tbi + versions = versions.mix(TABIX_BGZIPTABIX_INDEL.out.versions.first()) + TABIX_BGZIPTABIX_INDEL.out.gz_tbi .map { meta, file, index -> tuple(meta + [vartype: "indel"], file, index) } .set{split_indel_vcf} out_vcf_ch = out_vcf_ch.mix(split_indel_vcf) diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index fabe72f..6e64de8 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -1,10 +1,10 @@ { "-stub": { "content": [ - 76, + 62, { "BCFTOOLS_DEDUP": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_FILTER": { "bcftools": 1.2 @@ -13,13 +13,28 @@ "bcftools": 1.2 }, "BCFTOOLS_NORM": { - "bcftools": 1.18 + "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_1": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_2": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_3": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_4": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_QUERY": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 @@ -48,9 +63,6 @@ "RTGTOOLS_VCFEVAL": { "rtg-tools": "3.12.1" }, - "TABIX_TABIX": { - "tabix": "1.19.1" - }, "VCF_TO_CSV": { "python": "3.12.4" }, @@ -67,7 +79,8 @@ "small", "small/HG002", "small/HG002/preprocess", - "small/HG002/preprocess/HG002_GRCh38_CMRG_smallvar_v1.00.rh.norm.dedup.sort.vcf.gz", + "small/HG002/preprocess/HG002.vcf.gz", + "small/HG002/preprocess/HG002.vcf.gz.tbi", "small/HG002/stats", "small/HG002/stats/bcftools", "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", @@ -162,9 +175,8 @@ "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp.vcf.gz.tbi", "small/test1/benchmarks/rtgtools/test1.HG002.strelka.weighted_roc.tsv.gz", "small/test1/preprocess", - "small/test1/preprocess/HG002.strelka.variants.chr21.rh.norm.filter.vcf", - "small/test1/preprocess/test1.HG002.strelka.prepy.vcf.gz", - "small/test1/preprocess/test1.dedup.sort.vcf.gz", + "small/test1/preprocess/test1.vcf.gz", + "small/test1/preprocess/test1.vcf.gz.tbi", "small/test1/stats", "small/test1/stats/bcftools", "small/test1/stats/bcftools/test1.strelka.bcftools_stats.txt", @@ -197,9 +209,8 @@ "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp.vcf.gz.tbi", "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.weighted_roc.tsv.gz", "small/test2/preprocess", - "small/test2/preprocess/HG002.bcftools.chr21.rh.norm.filter.vcf", - "small/test2/preprocess/test2.HG002.bcftools.prepy.vcf.gz", - "small/test2/preprocess/test2.dedup.sort.vcf.gz", + "small/test2/preprocess/test2.vcf.gz", + "small/test2/preprocess/test2.vcf.gz.tbi", "small/test2/stats", "small/test2/stats/bcftools", "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" @@ -233,14 +244,14 @@ "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T13:40:17.22451061" + "timestamp": "2025-01-16T15:55:45.473583366" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ - 76, + 62, { "BCFTOOLS_DEDUP": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_FILTER": { "bcftools": 1.2 @@ -249,13 +260,28 @@ "bcftools": 1.2 }, "BCFTOOLS_NORM": { - "bcftools": 1.18 + "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_1": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_2": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_3": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_4": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_QUERY": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 @@ -284,9 +310,6 @@ "RTGTOOLS_VCFEVAL": { "rtg-tools": "3.12.1" }, - "TABIX_TABIX": { - "tabix": "1.19.1" - }, "VCF_TO_CSV": { "python": "3.12.4" }, @@ -324,7 +347,8 @@ "small", "small/HG002", "small/HG002/preprocess", - "small/HG002/preprocess/HG002_GRCh38_CMRG_smallvar_v1.00.rh.norm.dedup.sort.vcf.gz", + "small/HG002/preprocess/HG002.vcf.gz", + "small/HG002/preprocess/HG002.vcf.gz.tbi", "small/HG002/stats", "small/HG002/stats/bcftools", "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", @@ -493,9 +517,8 @@ "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp.vcf.gz.tbi", "small/test1/benchmarks/rtgtools/test1.HG002.strelka.weighted_roc.tsv.gz", "small/test1/preprocess", - "small/test1/preprocess/HG002.strelka.variants.chr21.rh.norm.filter.vcf", - "small/test1/preprocess/test1.HG002.strelka.prepy.vcf.gz", - "small/test1/preprocess/test1.dedup.sort.vcf.gz", + "small/test1/preprocess/test1.vcf.gz", + "small/test1/preprocess/test1.vcf.gz.tbi", "small/test1/stats", "small/test1/stats/bcftools", "small/test1/stats/bcftools/test1.strelka.bcftools_stats.txt", @@ -528,9 +551,8 @@ "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp.vcf.gz.tbi", "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.weighted_roc.tsv.gz", "small/test2/preprocess", - "small/test2/preprocess/HG002.bcftools.chr21.rh.norm.filter.vcf", - "small/test2/preprocess/test2.HG002.bcftools.prepy.vcf.gz", - "small/test2/preprocess/test2.dedup.sort.vcf.gz", + "small/test2/preprocess/test2.vcf.gz", + "small/test2/preprocess/test2.vcf.gz.tbi", "small/test2/stats", "small/test2/stats/bcftools", "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" @@ -552,33 +574,33 @@ "suffixIndex0:md5,b7bb2ba061ab54c0bf07c0a941d0277a", "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", - "HG002.bcftools_stats.txt:md5,b215fc0030c53bc8887e28b23b97efb6", - "test1.HG002.strelka.extended.csv:md5,4362260b357ac0221414095f4c5a8981", - "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a5ba4044a89ae80fb0ddf95147b5ae4c", - "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,52edef7d20ac8a7e03771037f5c93fe4", - "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,f71e697d7ebaf1d670e5dc2c0e0106d3", - "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,c388d5a15ba2ae8dff709b030f1b4828", - "test1.HG002.strelka.roc.all.csv.gz:md5,59ebbe78bf428b5c2c78c8ff92f54545", - "test1.HG002.strelka.summary.csv:md5,60af846379cf4fe078fbed6b9d1e8178", - "test1.HG002.strelka.phasing.txt:md5,838e67ae5b9cd9e218095596c03fbee3", - "test1.HG002.strelka.summary.txt:md5,e79779d3faebe02bae943ddc17c4cf91", - "test1.strelka.bcftools_stats.txt:md5,492f42090004470e7e0ea7abc5f89bdf", - "test2.HG002.bcftools.extended.csv:md5,2f8ef20f46c821333ba970e3034a6ccd", - "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,cdf3fdb7c5b4c54d9896e37a99dbf4f9", - "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,9b16abcfe483356020c550e1292554ed", - "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,f71e697d7ebaf1d670e5dc2c0e0106d3", - "test2.HG002.bcftools.roc.Locations.SNP.csv.gz:md5,c388d5a15ba2ae8dff709b030f1b4828", - "test2.HG002.bcftools.roc.all.csv.gz:md5,e772fefec84f9a6e60c6979bac14cedc", - "test2.HG002.bcftools.summary.csv:md5,05722d23f523141fdce842a18f1d8aa2", + "HG002.bcftools_stats.txt:md5,25a566bfca26275fbdce5d80b4c43d45", + "test1.HG002.strelka.extended.csv:md5,07297e4480e0b765b290a10f8bddbfe8", + "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,0f68dbeccf04d6bbe942ae90a70da219", + "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,f15e3ec73cba5af3632bd276e9d93278", + "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,aa2ccc4ceb4dcfc7173edcdc78b7b89b", + "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,73928333c290a527d8faccf22bd834fc", + "test1.HG002.strelka.roc.all.csv.gz:md5,cbf4db06f9cbdd0b3b3a29ee4bfc362b", + "test1.HG002.strelka.summary.csv:md5,8a5fff1b336e93cee21b3a81f49ab290", + "test1.HG002.strelka.phasing.txt:md5,8ed2c215bc14dc461a10e923d0127347", + "test1.HG002.strelka.summary.txt:md5,537e7469d43f94647018e173928f088e", + "test1.strelka.bcftools_stats.txt:md5,32586b4f0851fb4f386534a29fe8658f", + "test2.HG002.bcftools.extended.csv:md5,9ecb6d4bd55bc7e1ac18bd6cddaa15d9", + "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,8200b1ab6ab8d4665967ce24b28df103", + "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,b223c143e1e534c0c8381c1d1bd130a7", + "test2.HG002.bcftools.roc.Locations.SNP.PASS.csv.gz:md5,aa2ccc4ceb4dcfc7173edcdc78b7b89b", + "test2.HG002.bcftools.roc.Locations.SNP.csv.gz:md5,73928333c290a527d8faccf22bd834fc", + "test2.HG002.bcftools.roc.all.csv.gz:md5,c8799bbce78b88317baccbde04d453b1", + "test2.HG002.bcftools.summary.csv:md5,1a1e3471f9df31365787f09dcafbcb79", "test2.HG002.bcftools.phasing.txt:md5,38920536b8c3e241e873c07ba61762e6", - "test2.HG002.bcftools.summary.txt:md5,a7bbcdf86cd3f1f7815ea9bc25b57b61", - "test2.bcftools.bcftools_stats.txt:md5,440fd66ee557b215bbacd05953215f7a" + "test2.HG002.bcftools.summary.txt:md5,b8b6ba8ea69ecf241de01b347c226386", + "test2.bcftools.bcftools_stats.txt:md5,648803535cd0f6f94f52a2ad4587be8b" ] ], "meta": { "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T14:00:31.553072869" + "timestamp": "2025-01-17T09:31:09.115891055" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index 84d6427..63b16d5 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -1,28 +1,43 @@ { "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'": { "content": [ - 144, + 121, { "BCFTOOLS_DEDUP": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_FILTER": { "bcftools": 1.2 }, "BCFTOOLS_NORM": { - "bcftools": 1.18 + "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_1": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_2": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_3": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_4": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_QUERY": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 }, "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BGZIP_TABIX": { "tabix": 1.12 @@ -48,19 +63,13 @@ "SVYNC": { "svync": "0.1.2" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, "TABIX_BGZIP_QUERY": { "tabix": "1.19.1" }, "TABIX_BGZIP_TRUTH": { "tabix": "1.19.1" }, - "TABIX_TABIX": { + "TABIX_BGZIP_UNZIP": { "tabix": "1.19.1" }, "TRUVARI_BENCH": { @@ -85,7 +94,8 @@ "structural", "structural/HG002", "structural/HG002/preprocess", - "structural/HG002/preprocess/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.rh.norm.dedup.sort.vcf.gz", + "structural/HG002/preprocess/HG002.vcf.gz", + "structural/HG002/preprocess/HG002.vcf.gz.tbi", "structural/HG002/stats", "structural/HG002/stats/bcftools", "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", @@ -263,12 +273,8 @@ "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz", "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz.tbi", "structural/test1/preprocess", - "structural/test1/preprocess/manta.HG002.chr21.norm.sort.vcf.gz", - "structural/test1/preprocess/manta.HG002.chr21.norm.vcf.gz", - "structural/test1/preprocess/test1.dedup.sort.vcf.gz", - "structural/test1/preprocess/test1.manta.svync.vcf.gz", - "structural/test1/preprocess/test1.norm.filter.filter.vcf", - "structural/test1/preprocess/test1.norm.filter.vcf", + "structural/test1/preprocess/test1.vcf.gz", + "structural/test1/preprocess/test1.vcf.gz.tbi", "structural/test1/stats", "structural/test1/stats/bcftools", "structural/test1/stats/bcftools/test1.manta.bcftools_stats.txt", @@ -297,11 +303,8 @@ "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz", "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz.tbi", "structural/test2/preprocess", - "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.sort.vcf.gz", - "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.vcf.gz", - "structural/test2/preprocess/test2.dedup.sort.vcf.gz", - "structural/test2/preprocess/test2.norm.filter.filter.vcf", - "structural/test2/preprocess/test2.norm.filter.vcf", + "structural/test2/preprocess/test2.vcf.gz", + "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", "structural/test2/stats/bcftools", "structural/test2/stats/bcftools/test2.merged.bcftools_stats.txt", @@ -330,12 +333,8 @@ "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz", "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz.tbi", "structural/test3/preprocess", - "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.sort.vcf.gz", - "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", - "structural/test3/preprocess/test3.dedup.sort.vcf.gz", - "structural/test3/preprocess/test3.dragen.svync.vcf.gz", - "structural/test3/preprocess/test3.norm.filter.filter.vcf", - "structural/test3/preprocess/test3.norm.filter.vcf", + "structural/test3/preprocess/test3.vcf.gz", + "structural/test3/preprocess/test3.vcf.gz.tbi", "structural/test3/stats", "structural/test3/stats/bcftools", "structural/test3/stats/bcftools/test3.dragen.bcftools_stats.txt", @@ -343,19 +342,19 @@ "structural/test3/stats/survivor/test3.dragen_mqc.stats" ], [ - "HG002.bcftools_stats.txt:md5,8294f172a72ca7219a32db9c27e2524c", + "HG002.bcftools_stats.txt:md5,06ee97dcd1b34ddca03a7e5f9af23e68", "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", "test1.HG002.manta.distances:md5,346f18a5cbeece98716951c8fc2aaea4", "test1.HG002.manta.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", - "test1.manta.bcftools_stats.txt:md5,7d65792aa3a84de09675facf62135c93", + "test1.manta.bcftools_stats.txt:md5,9a82df362fe77db3330544f2b98b439e", "test1.manta_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", "test2.HG002.merged.distances:md5,346f18a5cbeece98716951c8fc2aaea4", "test2.HG002.merged.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", - "test2.merged.bcftools_stats.txt:md5,1445742129b0ee67d8706af3dcf0ab2d", + "test2.merged.bcftools_stats.txt:md5,bbd579cedaf78b6199fb71e52acfc14d", "test2.merged_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", "test3.HG002.dragen.distances:md5,346f18a5cbeece98716951c8fc2aaea4", "test3.HG002.dragen.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", - "test3.dragen.bcftools_stats.txt:md5,5d2b48ac5f194f5a2cf01b9623a28cce", + "test3.dragen.bcftools_stats.txt:md5,73648a9eb3b6cd5049f579ef861637b2", "test3.dragen_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b" ] ], @@ -363,32 +362,47 @@ "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T13:53:07.620717905" + "timestamp": "2025-01-17T09:36:16.512872909" }, "-stub": { "content": [ - 144, + 121, { "BCFTOOLS_DEDUP": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_FILTER": { "bcftools": 1.2 }, "BCFTOOLS_NORM": { - "bcftools": 1.18 + "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_1": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_2": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_3": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_4": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_QUERY": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 }, "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BGZIP_TABIX": { "tabix": 1.12 @@ -414,19 +428,13 @@ "SVYNC": { "svync": "0.1.2" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, "TABIX_BGZIP_QUERY": { "tabix": "1.19.1" }, "TABIX_BGZIP_TRUTH": { "tabix": "1.19.1" }, - "TABIX_TABIX": { + "TABIX_BGZIP_UNZIP": { "tabix": "1.19.1" }, "TRUVARI_BENCH": { @@ -451,7 +459,8 @@ "structural", "structural/HG002", "structural/HG002/preprocess", - "structural/HG002/preprocess/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.rh.norm.dedup.sort.vcf.gz", + "structural/HG002/preprocess/HG002.vcf.gz", + "structural/HG002/preprocess/HG002.vcf.gz.tbi", "structural/HG002/stats", "structural/HG002/stats/bcftools", "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", @@ -568,12 +577,8 @@ "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz", "structural/test1/benchmarks/wittyer/test1.HG002.manta.vcf.gz.tbi", "structural/test1/preprocess", - "structural/test1/preprocess/manta.HG002.chr21.norm.sort.vcf.gz", - "structural/test1/preprocess/manta.HG002.chr21.norm.vcf.gz", - "structural/test1/preprocess/test1.dedup.sort.vcf.gz", - "structural/test1/preprocess/test1.manta.svync.vcf.gz", - "structural/test1/preprocess/test1.norm.filter.filter.vcf", - "structural/test1/preprocess/test1.norm.filter.vcf", + "structural/test1/preprocess/test1.vcf.gz", + "structural/test1/preprocess/test1.vcf.gz.tbi", "structural/test1/stats", "structural/test1/stats/bcftools", "structural/test1/stats/bcftools/test1.manta.bcftools_stats.txt", @@ -602,11 +607,8 @@ "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz", "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz.tbi", "structural/test2/preprocess", - "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.sort.vcf.gz", - "structural/test2/preprocess/Ashkenazim_HG002.filtered.sv.chr21.norm.vcf.gz", - "structural/test2/preprocess/test2.dedup.sort.vcf.gz", - "structural/test2/preprocess/test2.norm.filter.filter.vcf", - "structural/test2/preprocess/test2.norm.filter.vcf", + "structural/test2/preprocess/test2.vcf.gz", + "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", "structural/test2/stats/bcftools", "structural/test2/stats/bcftools/test2.merged.bcftools_stats.txt", @@ -635,12 +637,8 @@ "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz", "structural/test3/benchmarks/wittyer/test3.HG002.dragen.vcf.gz.tbi", "structural/test3/preprocess", - "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.sort.vcf.gz", - "structural/test3/preprocess/HG002_DRAGEN_SV_hg19.chr21.norm.vcf.gz", - "structural/test3/preprocess/test3.dedup.sort.vcf.gz", - "structural/test3/preprocess/test3.dragen.svync.vcf.gz", - "structural/test3/preprocess/test3.norm.filter.filter.vcf", - "structural/test3/preprocess/test3.norm.filter.vcf", + "structural/test3/preprocess/test3.vcf.gz", + "structural/test3/preprocess/test3.vcf.gz.tbi", "structural/test3/stats", "structural/test3/stats/bcftools", "structural/test3/stats/bcftools/test3.dragen.bcftools_stats.txt", @@ -668,6 +666,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T13:55:36.985456265" + "timestamp": "2025-01-16T15:43:53.130315988" } } \ No newline at end of file diff --git a/tests/liftover_test.nf.test.snap b/tests/liftover_test.nf.test.snap index 023d23d..d2ec354 100644 --- a/tests/liftover_test.nf.test.snap +++ b/tests/liftover_test.nf.test.snap @@ -1,19 +1,31 @@ { "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover 'test'": { "content": [ - 67, + 57, { - "BCFTOOLS_FILTER": { + "BCFTOOLS_REHEADER_1": { "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_2": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_3": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_4": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_QUERY": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 }, "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BGZIP_TABIX": { "tabix": 1.12 @@ -39,10 +51,7 @@ "TABIX_BGZIP": { "tabix": "1.19.1" }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, - "TABIX_TABIX": { + "TABIX_BGZIP_UNZIP": { "tabix": "1.19.1" }, "TRUVARI_BENCH": { @@ -63,8 +72,9 @@ "references/dictionary/genome.dict", "structural", "structural/HG002", - "structural/HG002/liftover", - "structural/HG002/liftover/test2.renamechr.vcf.gz", + "structural/HG002/preprocess", + "structural/HG002/preprocess/HG002.vcf.gz", + "structural/HG002/preprocess/HG002.vcf.gz.tbi", "structural/HG002/stats", "structural/HG002/stats/bcftools", "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", @@ -167,8 +177,8 @@ "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz", "structural/test1/benchmarks/truvari/test1.HG002.delly.tp-comp.vcf.gz.tbi", "structural/test1/preprocess", - "structural/test1/preprocess/test1.filter.filter.vcf", - "structural/test1/preprocess/test1.filter.vcf", + "structural/test1/preprocess/test1.vcf.gz", + "structural/test1/preprocess/test1.vcf.gz.tbi", "structural/test1/stats", "structural/test1/stats/bcftools", "structural/test1/stats/bcftools/test1.delly.bcftools_stats.txt", @@ -186,9 +196,12 @@ "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-base.vcf.gz.tbi", "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz", "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz.tbi", + "structural/test2/preporcess", + "structural/test2/preporcess/liftover", + "structural/test2/preporcess/liftover/test2.reformattedrenamecr.vcf.gz", "structural/test2/preprocess", - "structural/test2/preprocess/test2.filter.filter.vcf", - "structural/test2/preprocess/test2.filter.vcf", + "structural/test2/preprocess/test2.vcf.gz", + "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", "structural/test2/stats/bcftools", "structural/test2/stats/bcftools/test2.manta.bcftools_stats.txt", @@ -196,18 +209,18 @@ "structural/test2/stats/survivor/test2.manta_mqc.stats" ], [ - "HG002.bcftools_stats.txt:md5,7c007a87b5730787e570712e784d3cc3", + "HG002.bcftools_stats.txt:md5,142c1fab782c9eb2aa932440e1589461", "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "test1.delly.bcftools_stats.txt:md5,aace5a23af8b02f56bcaf03722f9bb50", - "test1.delly_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", - "test2.manta.bcftools_stats.txt:md5,1e700076bd303ee079b23658485eb7d4", - "test2.manta_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b" + "test1.delly.bcftools_stats.txt:md5,f204ab10d82b3cbc9ee51cb6ad548121", + "test1.delly_mqc.stats:md5,e140ad55975c767578b0dd6aff58ba29", + "test2.manta.bcftools_stats.txt:md5,7377c6a71039ef22479126d794d8bbe1", + "test2.manta_mqc.stats:md5,1d74b41c6b970992e3a39682b0a68e23" ] ], "meta": { "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T14:41:30.39094733" + "timestamp": "2025-01-17T08:25:51.92716226" } } \ No newline at end of file diff --git a/tests/liftover_truth.nf.test.snap b/tests/liftover_truth.nf.test.snap index b6d9288..98508cc 100644 --- a/tests/liftover_truth.nf.test.snap +++ b/tests/liftover_truth.nf.test.snap @@ -1,31 +1,46 @@ { "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover 'truth'": { "content": [ - 85, + 68, { "BCFTOOLS_DEDUP": { - "bcftools": 1.18 - }, - "BCFTOOLS_FILTER": { "bcftools": 1.2 }, "BCFTOOLS_MERGE": { "bcftools": 1.2 }, "BCFTOOLS_NORM": { - "bcftools": 1.18 + "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_1": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_2": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_3": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_4": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_QUERY": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 + }, + "BCFTOOLS_SPLIT_MULTI": { + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 }, "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 + "bcftools": 1.2 }, "DATAVZRD": { "datavzrd": "2.36.12" @@ -48,12 +63,6 @@ "RTGTOOLS_VCFEVAL": { "rtg-tools": "3.12.1" }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, - "TABIX_TABIX": { - "tabix": "1.19.1" - }, "UCSC_LIFTOVER": { "ucsc": 377 }, @@ -95,11 +104,12 @@ "references/rtgtools/genome.sdf/summary.txt", "small", "small/HG002", - "small/HG002/liftover", - "small/HG002/liftover/HG002.renamechr.vcf.gz", - "small/HG002/liftover/HG002.sort.merged.bed", "small/HG002/preprocess", - "small/HG002/preprocess/HG002.renamechr.rh.norm.dedup.sort.vcf.gz", + "small/HG002/preprocess/HG002.vcf.gz", + "small/HG002/preprocess/HG002.vcf.gz.tbi", + "small/HG002/preprocess/liftover", + "small/HG002/preprocess/liftover/HG002.reformatted.renamechr.vcf.gz", + "small/HG002/preprocess/liftover/HG002_GRCh37_1_22_v4.2.1_highconf.liftedsort.merged.bed", "small/HG002/stats", "small/HG002/stats/bcftools", "small/HG002/stats/bcftools/HG002.bcftools_stats.txt", @@ -268,8 +278,8 @@ "small/test1/benchmarks/rtgtools/test1.HG002.strelka.tp.vcf.gz.tbi", "small/test1/benchmarks/rtgtools/test1.HG002.strelka.weighted_roc.tsv.gz", "small/test1/preprocess", - "small/test1/preprocess/test1.dedup.sort.vcf.gz", - "small/test1/preprocess/test1.norm.filter.vcf", + "small/test1/preprocess/test1.vcf.gz", + "small/test1/preprocess/test1.vcf.gz.tbi", "small/test1/stats", "small/test1/stats/bcftools", "small/test1/stats/bcftools/test1.strelka.bcftools_stats.txt", @@ -302,8 +312,8 @@ "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.tp.vcf.gz.tbi", "small/test2/benchmarks/rtgtools/test2.HG002.bcftools.weighted_roc.tsv.gz", "small/test2/preprocess", - "small/test2/preprocess/test2.dedup.sort.vcf.gz", - "small/test2/preprocess/test2.norm.filter.vcf", + "small/test2/preprocess/test2.vcf.gz", + "small/test2/preprocess/test2.vcf.gz.tbi", "small/test2/stats", "small/test2/stats/bcftools", "small/test2/stats/bcftools/test2.bcftools.bcftools_stats.txt" @@ -325,18 +335,18 @@ "suffixIndex0:md5,b7bb2ba061ab54c0bf07c0a941d0277a", "suffixdata0:md5,f2876dd730673cd49c4de191001f634e", "suffixpointer0:md5,468281ffb10d7dd934289af762a03781", - "HG002.sort.merged.bed:md5,5e997133249c2227cb5363b314245636", - "HG002.bcftools_stats.txt:md5,07fafa430abc857969015ea6a41d1032", - "test1.HG002.strelka.extended.csv:md5,111a236ca6b747face70e8111c09c51f", - "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,5425b34f4688dda4d55f9f7a73667b18", - "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,eb9834ec5660d1e32ade0c856b9cfb7c", - "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,778eb91f54cb830fb91fcddc688668e0", - "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,516a4b58814cf32245a5b2f2c0445c8e", - "test1.HG002.strelka.roc.all.csv.gz:md5,a69fb6ae5cd051d4607da0224009f1d1", - "test1.HG002.strelka.summary.csv:md5,d29cba645e161dbdeb6247f57fce5780", - "test1.HG002.strelka.phasing.txt:md5,38920536b8c3e241e873c07ba61762e6", - "test1.HG002.strelka.summary.txt:md5,6c43a0d1ad065f851942828259d551a5", - "test1.strelka.bcftools_stats.txt:md5,7d65792aa3a84de09675facf62135c93", + "HG002_GRCh37_1_22_v4.2.1_highconf.liftedsort.merged.bed:md5,5e997133249c2227cb5363b314245636", + "HG002.bcftools_stats.txt:md5,db248c6e7e92253abd836c1d8a326371", + "test1.HG002.strelka.extended.csv:md5,fadf06276179c1cc388007e5651fb9af", + "test1.HG002.strelka.roc.Locations.INDEL.PASS.csv.gz:md5,a08665be8ea20855a14d6418d07521dc", + "test1.HG002.strelka.roc.Locations.INDEL.csv.gz:md5,8d4a20a5914379230952aeb252845e2d", + "test1.HG002.strelka.roc.Locations.SNP.PASS.csv.gz:md5,c546609a3e23b4c5c97ee0fc2e864e07", + "test1.HG002.strelka.roc.Locations.SNP.csv.gz:md5,8d2600c17665ed1299e2dd483705ddaa", + "test1.HG002.strelka.roc.all.csv.gz:md5,d0a4408438b2182cb82caec918c090f1", + "test1.HG002.strelka.summary.csv:md5,001fa2371777d1d2dc3eb0f4dcaca197", + "test1.HG002.strelka.phasing.txt:md5,0c9340e64e032b5f74d0ea3773464afd", + "test1.HG002.strelka.summary.txt:md5,4b775edb878759727ef66d5d5b2b9ee8", + "test1.strelka.bcftools_stats.txt:md5,869e7eac4d1bde15c33ac3598af3b58e", "test2.HG002.bcftools.extended.csv:md5,05c6efd85a9b9823a5ddfe6619be3323", "test2.HG002.bcftools.roc.Locations.INDEL.PASS.csv.gz:md5,463cee548b7bdf133c0ae9b5803a50ca", "test2.HG002.bcftools.roc.Locations.INDEL.csv.gz:md5,424e39c327ae6ef9001942bc895abdc9", @@ -346,13 +356,13 @@ "test2.HG002.bcftools.summary.csv:md5,38f3d8eb32c4d006a5623777ad265c39", "test2.HG002.bcftools.phasing.txt:md5,38920536b8c3e241e873c07ba61762e6", "test2.HG002.bcftools.summary.txt:md5,264eb2b064a9ee4b098470a13c2887c9", - "test2.bcftools.bcftools_stats.txt:md5,57aff2f0a6f830e920869b987502a343" + "test2.bcftools.bcftools_stats.txt:md5,2efc3e61c4aaa5bb2da48803c81d8fcc" ] ], "meta": { "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T14:48:00.444469734" + "timestamp": "2025-01-17T09:02:13.833941457" } } \ No newline at end of file diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 5f59ce6..a446e99 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -1,28 +1,28 @@ { "Params: --analysis 'somatic' --variant_type 'indel' --method 'sompy'": { "content": [ - 27, + 21, { - "BCFTOOLS_FILTER": { + "BCFTOOLS_REHEADER_QUERY": { "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 }, "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_VIEW_FILTERMISSING": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_VIEW_SUBSAMPLE": { - "bcftools": 1.18 + "bcftools": 1.2 }, "DATAVZRD": { "datavzrd": "2.36.12" @@ -36,9 +36,6 @@ "PLOTS": { "r-base": "4.3.1" }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, "Workflow": { "nf-core/variantbenchmarking": "v1.0dev" } @@ -46,6 +43,9 @@ [ "indel", "indel/SEQC2", + "indel/SEQC2/preprocess", + "indel/SEQC2/preprocess/SEQC2.vcf.gz", + "indel/SEQC2/preprocess/SEQC2.vcf.gz.tbi", "indel/SEQC2/stats", "indel/SEQC2/stats/bcftools", "indel/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", @@ -151,7 +151,8 @@ "indel/test1/benchmarks/sompy/test1.SEQC2.freebayes.metrics.json", "indel/test1/benchmarks/sompy/test1.SEQC2.freebayes.stats.csv", "indel/test1/preprocess", - "indel/test1/preprocess/test1.filter.vcf", + "indel/test1/preprocess/test1.vcf.gz", + "indel/test1/preprocess/test1.vcf.gz.tbi", "indel/test1/stats", "indel/test1/stats/bcftools", "indel/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", @@ -162,8 +163,8 @@ "indel/test2/benchmarks/sompy/test2.SEQC2.strelka.metrics.json", "indel/test2/benchmarks/sompy/test2.SEQC2.strelka.stats.csv", "indel/test2/preprocess", - "indel/test2/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_indels.sort.vcf.gz", - "indel/test2/preprocess/test2.filter.vcf", + "indel/test2/preprocess/test2.vcf.gz", + "indel/test2/preprocess/test2.vcf.gz.tbi", "indel/test2/stats", "indel/test2/stats/bcftools", "indel/test2/stats/bcftools/test2.strelka.bcftools_stats.txt", @@ -171,15 +172,15 @@ "pipeline_info/nf_core_pipeline_software_mqc_versions.yml" ], [ - "SEQC2.bcftools_stats.txt:md5,e530daf4f6a4923f1cd85c51893d5747", - "test1.freebayes.bcftools_stats.txt:md5,c5025b5c6256c1d808badee73a7c220c", - "test2.strelka.bcftools_stats.txt:md5,1e700076bd303ee079b23658485eb7d4" + "SEQC2.bcftools_stats.txt:md5,97beec3b6ce6471625018d91c75df08d", + "test1.freebayes.bcftools_stats.txt:md5,ca4f9a72e5cb852b78dbdf92142a4a6f", + "test2.strelka.bcftools_stats.txt:md5,a098fdaee64c05cd8e9efcc13c2b374d" ] ], "meta": { "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T14:32:00.898226798" + "timestamp": "2025-01-17T09:20:01.232442793" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 45b7e8c..765e3f6 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -1,28 +1,28 @@ { "-stub": { "content": [ - 38, + 29, { - "BCFTOOLS_FILTER": { + "BCFTOOLS_REHEADER_QUERY": { "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 }, "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_VIEW_FILTERMISSING": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_VIEW_SUBSAMPLE": { - "bcftools": 1.18 + "bcftools": 1.2 }, "DATAVZRD": { "datavzrd": "2.36.12" @@ -36,9 +36,6 @@ "PLOTS": { "r-base": "4.3.1" }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, "Workflow": { "nf-core/variantbenchmarking": "v1.0dev" } @@ -48,6 +45,9 @@ "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "snv", "snv/SEQC2", + "snv/SEQC2/preprocess", + "snv/SEQC2/preprocess/SEQC2.vcf.gz", + "snv/SEQC2/preprocess/SEQC2.vcf.gz.tbi", "snv/SEQC2/stats", "snv/SEQC2/stats/bcftools", "snv/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", @@ -91,7 +91,8 @@ "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.metrics.json", "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.stats.csv", "snv/test1/preprocess", - "snv/test1/preprocess/test1.filter.vcf", + "snv/test1/preprocess/test1.vcf.gz", + "snv/test1/preprocess/test1.vcf.gz.tbi", "snv/test1/stats", "snv/test1/stats/bcftools", "snv/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", @@ -102,8 +103,8 @@ "snv/test2/benchmarks/sompy/test2.SEQC2.manta.metrics.json", "snv/test2/benchmarks/sompy/test2.SEQC2.manta.stats.csv", "snv/test2/preprocess", - "snv/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", - "snv/test2/preprocess/test2.filter.vcf", + "snv/test2/preprocess/test2.vcf.gz", + "snv/test2/preprocess/test2.vcf.gz.tbi", "snv/test2/stats", "snv/test2/stats/bcftools", "snv/test2/stats/bcftools/test2.manta.bcftools_stats.txt", @@ -114,8 +115,8 @@ "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.metrics.json", "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.stats.csv", "snv/test3/preprocess", - "snv/test3/preprocess/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.sort.vcf.gz", - "snv/test3/preprocess/test3.filter.vcf", + "snv/test3/preprocess/test3.vcf.gz", + "snv/test3/preprocess/test3.vcf.gz.tbi", "snv/test3/stats", "snv/test3/stats/bcftools", "snv/test3/stats/bcftools/test3.strelka.bcftools_stats.txt" @@ -131,6 +132,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T14:15:33.922876808" + "timestamp": "2025-01-17T09:17:06.41202116" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index f5cf4b6..c495cc5 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -1,28 +1,40 @@ { "Params: --analysis 'somatic' --variant_type 'structural' --method 'truvari,svbenchmark'": { "content": [ - 69, + 53, { - "BCFTOOLS_FILTER": { + "BCFTOOLS_REHEADER_1": { "bcftools": 1.2 }, - "BCFTOOLS_REHEADER": { - "bcftools": 1.18 + "BCFTOOLS_REHEADER_2": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_3": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_4": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_QUERY": { + "bcftools": 1.2 + }, + "BCFTOOLS_REHEADER_TRUTH": { + "bcftools": 1.2 }, "BCFTOOLS_SORT": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_STATS": { "bcftools": 1.18 }, "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_VIEW_FILTERMISSING": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BCFTOOLS_VIEW_SUBSAMPLE": { - "bcftools": 1.18 + "bcftools": 1.2 }, "BGZIP_TABIX": { "tabix": 1.12 @@ -42,13 +54,7 @@ "SURVIVOR_STATS": { "survivor": "1.0.7" }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, - "TABIX_BGZIPTABIX": { - "tabix": "1.19.1" - }, - "TABIX_TABIX": { + "TABIX_BGZIP_UNZIP": { "tabix": "1.19.1" }, "TRUVARI_BENCH": { @@ -66,6 +72,9 @@ "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", "structural", "structural/SEQC2", + "structural/SEQC2/preprocess", + "structural/SEQC2/preprocess/SEQC2.vcf.gz", + "structural/SEQC2/preprocess/SEQC2.vcf.gz.tbi", "structural/SEQC2/stats", "structural/SEQC2/stats/bcftools", "structural/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", @@ -74,6 +83,10 @@ "structural/multiqc", "structural/multiqc/multiqc_data", "structural/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", "structural/multiqc/multiqc_data/multiqc.log", "structural/multiqc/multiqc_data/multiqc_bcftools_stats.txt", "structural/multiqc/multiqc_data/multiqc_citations.txt", @@ -85,16 +98,28 @@ "structural/multiqc/multiqc_plots", "structural/multiqc/multiqc_plots/pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", "structural/multiqc/multiqc_plots/pdf/general_stats_table.pdf", "structural/multiqc/multiqc_plots/pdf/survivor-cnt.pdf", "structural/multiqc/multiqc_plots/pdf/survivor-pct.pdf", "structural/multiqc/multiqc_plots/png", "structural/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", "structural/multiqc/multiqc_plots/png/general_stats_table.png", "structural/multiqc/multiqc_plots/png/survivor-cnt.png", "structural/multiqc/multiqc_plots/png/survivor-pct.png", "structural/multiqc/multiqc_plots/svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", "structural/multiqc/multiqc_plots/svg/general_stats_table.svg", "structural/multiqc/multiqc_plots/svg/survivor-cnt.svg", "structural/multiqc/multiqc_plots/svg/survivor-pct.svg", @@ -152,9 +177,8 @@ "structural/test1/benchmarks/truvari/test1.SEQC2.tiddit.tp-comp.vcf.gz", "structural/test1/benchmarks/truvari/test1.SEQC2.tiddit.tp-comp.vcf.gz.tbi", "structural/test1/preprocess", - "structural/test1/preprocess/HCC1395T_vs_HCC1395N.tiddit_sv_merge.sort.vcf.gz", - "structural/test1/preprocess/test1.filter.filter.vcf", - "structural/test1/preprocess/test1.filter.vcf", + "structural/test1/preprocess/test1.vcf.gz", + "structural/test1/preprocess/test1.vcf.gz.tbi", "structural/test1/stats", "structural/test1/stats/bcftools", "structural/test1/stats/bcftools/test1.tiddit.bcftools_stats.txt", @@ -173,9 +197,8 @@ "structural/test2/benchmarks/truvari/test2.SEQC2.manta.tp-comp.vcf.gz", "structural/test2/benchmarks/truvari/test2.SEQC2.manta.tp-comp.vcf.gz.tbi", "structural/test2/preprocess", - "structural/test2/preprocess/HCC1395T_vs_HCC1395N.manta.somatic_sv.sort.vcf.gz", - "structural/test2/preprocess/test2.filter.filter.vcf", - "structural/test2/preprocess/test2.filter.vcf", + "structural/test2/preprocess/test2.vcf.gz", + "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", "structural/test2/stats/bcftools", "structural/test2/stats/bcftools/test2.manta.bcftools_stats.txt", @@ -183,18 +206,18 @@ "structural/test2/stats/survivor/test2.manta_mqc.stats" ], [ - "SEQC2.bcftools_stats.txt:md5,a60de9c4d2f3db87d8ae404b73859bfa", + "SEQC2.bcftools_stats.txt:md5,4830a787124a30a79fb0ec3c1d8c6206", "SEQC2_mqc.stats:md5,a1327ec0cd3131f9e16cccf0024f61a1", - "test1.tiddit.bcftools_stats.txt:md5,aace5a23af8b02f56bcaf03722f9bb50", - "test1.tiddit_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", - "test2.manta.bcftools_stats.txt:md5,1e700076bd303ee079b23658485eb7d4", - "test2.manta_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b" + "test1.tiddit.bcftools_stats.txt:md5,cbd62a9be5344b2d6223f8600483e6a2", + "test1.tiddit_mqc.stats:md5,0eb749429a6072f6bf63d377e37514d1", + "test2.manta.bcftools_stats.txt:md5,1926259ac4ad828a4a50a8e07e2b1d6f", + "test2.manta_mqc.stats:md5,73bc60cd2754202ddca8be6552e85ffa" ] ], "meta": { "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-13T14:18:00.311055864" + "timestamp": "2025-01-17T09:27:15.598502393" } } \ No newline at end of file From fa9eb7e7f2f662e501cf79e99ddda390220d7e00 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 17 Jan 2025 10:25:34 +0000 Subject: [PATCH 241/292] renew snapshot --- tests/liftover_test.nf.test.snap | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/tests/liftover_test.nf.test.snap b/tests/liftover_test.nf.test.snap index d2ec354..44a77ed 100644 --- a/tests/liftover_test.nf.test.snap +++ b/tests/liftover_test.nf.test.snap @@ -198,7 +198,7 @@ "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz.tbi", "structural/test2/preporcess", "structural/test2/preporcess/liftover", - "structural/test2/preporcess/liftover/test2.reformattedrenamecr.vcf.gz", + "structural/test2/preporcess/liftover/test2.reformatted.renamechr.vcf.gz", "structural/test2/preprocess", "structural/test2/preprocess/test2.vcf.gz", "structural/test2/preprocess/test2.vcf.gz.tbi", @@ -213,7 +213,7 @@ "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", "test1.delly.bcftools_stats.txt:md5,f204ab10d82b3cbc9ee51cb6ad548121", "test1.delly_mqc.stats:md5,e140ad55975c767578b0dd6aff58ba29", - "test2.manta.bcftools_stats.txt:md5,7377c6a71039ef22479126d794d8bbe1", + "test2.manta.bcftools_stats.txt:md5,0de1446278a5448b7dc5a02bcbf927a4", "test2.manta_mqc.stats:md5,1d74b41c6b970992e3a39682b0a68e23" ] ], @@ -221,6 +221,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.3" }, - "timestamp": "2025-01-17T08:25:51.92716226" + "timestamp": "2025-01-17T10:22:18.275352814" } } \ No newline at end of file From dee60ae6af06d0aeff1c43a6fbb01003f49ba033 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 20 Jan 2025 14:36:47 +0000 Subject: [PATCH 242/292] Template update for nf-core/tools version 3.1.2 --- .github/workflows/ci.yml | 2 ++ .github/workflows/download_pipeline.yml | 28 ++++++++------- .nf-core.yml | 2 +- CITATIONS.md | 4 ++- README.md | 10 ++++-- conf/test.config | 4 +-- nextflow.config | 2 +- ro-crate-metadata.json | 48 ++++++++----------------- 8 files changed, 45 insertions(+), 55 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index ef4cd4d..2b51a52 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -46,6 +46,8 @@ jobs: steps: - name: Check out pipeline code uses: actions/checkout@11bd71901bbe5b1630ceea73d27597364c9af683 # v4 + with: + fetch-depth: 0 - name: Set up Nextflow uses: nf-core/setup-nextflow@v2 diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 13b51e2..ab06316 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -34,6 +34,17 @@ jobs: REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }} REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }} REPO_BRANCH: ${{ steps.get_repo_properties.outputs.REPO_BRANCH }} + steps: + - name: Get the repository name and current branch + id: get_repo_properties + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT" + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT" + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT" + + download: + runs-on: ubuntu-latest + needs: configure steps: - name: Install Nextflow uses: nf-core/setup-nextflow@v2 @@ -56,21 +67,10 @@ jobs: python -m pip install --upgrade pip pip install git+https://github.com/nf-core/tools.git@dev - - name: Get the repository name and current branch set as environment variable - id: get_repo_properties - run: | - echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT" - echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT" - echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT" - - name: Make a cache directory for the container images run: | mkdir -p ./singularity_container_images - download: - runs-on: ubuntu-latest - needs: configure - steps: - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./singularity_container_images @@ -87,6 +87,9 @@ jobs: - name: Inspect download run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} + - name: Inspect container images + run: tree ./singularity_container_images | tee ./container_initial + - name: Count the downloaded number of container images id: count_initial run: | @@ -123,7 +126,8 @@ jobs: final_count=${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }} difference=$((final_count - initial_count)) echo "$difference additional container images were \n downloaded at runtime . The pipeline has no support for offline runs!" - tree ./singularity_container_images + tree ./singularity_container_images > ./container_afterwards + diff ./container_initial ./container_afterwards exit 1 else echo "The pipeline can be downloaded successfully!" diff --git a/.nf-core.yml b/.nf-core.yml index 148d2aa..6f583e4 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,6 @@ lint: actions_ci: false -nf_core_version: 3.1.1 +nf_core_version: 3.1.2 repository_type: pipeline template: author: kuebra.narci@dkfz.de diff --git a/CITATIONS.md b/CITATIONS.md index 4d44a0c..44ca0a0 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,7 +12,9 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) -> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) +> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. + +- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. diff --git a/README.md b/README.md index cba9213..37523ad 100644 --- a/README.md +++ b/README.md @@ -3,7 +3,9 @@ nf-core/variantbenchmarking -[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml) + + +[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) @@ -32,7 +34,7 @@ ## Usage > [!NOTE] -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data. - + + + An extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file. diff --git a/conf/test.config b/conf/test.config index 1adb3c8..cd52c58 100644 --- a/conf/test.config +++ b/conf/test.config @@ -25,8 +25,6 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' - - // Genome references + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv'// Genome references genome = 'R64-1-1' } diff --git a/nextflow.config b/nextflow.config index d7b17cf..53b78d2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -249,7 +249,7 @@ manifest { // Nextflow plugins plugins { - id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-schema@2.3.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet } validation { diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json index b7ccc99..db7990a 100644 --- a/ro-crate-metadata.json +++ b/ro-crate-metadata.json @@ -22,8 +22,8 @@ "@id": "./", "@type": "Dataset", "creativeWorkStatus": "InProgress", - "datePublished": "2024-12-20T13:10:59+00:00", - "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    [![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is a bioinformatics pipeline that ...\n\n\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow.Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n\n\nNow, you can run the pipeline using:\n\n\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", + "datePublished": "2025-01-20T14:36:33+00:00", + "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    \n\n[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is a bioinformatics pipeline that ...\n\n\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n\n\nNow, you can run the pipeline using:\n\n\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", "hasPart": [ { "@id": "main.nf" @@ -99,7 +99,7 @@ }, "mentions": [ { - "@id": "#91c4f0d3-aae1-4ff3-b0fc-040d23c0493d" + "@id": "#6051d38b-d786-4206-bf51-525cae6a07df" } ], "name": "nf-core/variantbenchmarking" @@ -121,41 +121,21 @@ }, { "@id": "main.nf", - "@type": [ - "File", - "SoftwareSourceCode", - "ComputationalWorkflow" - ], + "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"], "dateCreated": "", - "dateModified": "2024-12-20T13:10:59Z", + "dateModified": "2025-01-20T14:36:33Z", "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/", - "keywords": [ - "nf-core", - "nextflow", - "benchmark", - "draft", - "structural-variants", - "variant-calling" - ], - "license": [ - "MIT" - ], - "name": [ - "nf-core/variantbenchmarking" - ], + "keywords": ["nf-core", "nextflow", "benchmark", "draft", "structural-variants", "variant-calling"], + "license": ["MIT"], + "name": ["nf-core/variantbenchmarking"], "programmingLanguage": { "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow" }, "sdPublisher": { "@id": "https://nf-co.re/" }, - "url": [ - "https://github.com/nf-core/variantbenchmarking", - "https://nf-co.re/variantbenchmarking/dev/" - ], - "version": [ - "1.0dev" - ] + "url": ["https://github.com/nf-core/variantbenchmarking", "https://nf-co.re/variantbenchmarking/dev/"], + "version": ["1.0dev"] }, { "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow", @@ -170,11 +150,11 @@ "version": "!>=24.04.2" }, { - "@id": "#91c4f0d3-aae1-4ff3-b0fc-040d23c0493d", + "@id": "#6051d38b-d786-4206-bf51-525cae6a07df", "@type": "TestSuite", "instance": [ { - "@id": "#68e56bc1-38c8-4238-b744-2a682abe9825" + "@id": "#df38e5e1-380b-45f5-978b-955d38f83885" } ], "mainEntity": { @@ -183,7 +163,7 @@ "name": "Test suite for nf-core/variantbenchmarking" }, { - "@id": "#68e56bc1-38c8-4238-b744-2a682abe9825", + "@id": "#df38e5e1-380b-45f5-978b-955d38f83885", "@type": "TestInstance", "name": "GitHub Actions workflow for testing nf-core/variantbenchmarking", "resource": "repos/nf-core/variantbenchmarking/actions/workflows/ci.yml", @@ -312,4 +292,4 @@ "url": "https://nf-co.re/" } ] -} \ No newline at end of file +} From a470e89930f856f41dfcccc6c9a34ea99e7cfa1d Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 22 Jan 2025 11:22:05 +0000 Subject: [PATCH 243/292] new containers --- modules/local/merge_reports/environment.yml | 9 +++++---- modules/local/merge_reports/main.nf | 4 ++-- 2 files changed, 7 insertions(+), 6 deletions(-) diff --git a/modules/local/merge_reports/environment.yml b/modules/local/merge_reports/environment.yml index eeca6d3..c3361a6 100644 --- a/modules/local/merge_reports/environment.yml +++ b/modules/local/merge_reports/environment.yml @@ -1,6 +1,7 @@ -name: merge_reports channels: - - defaults + - conda-forge + - bioconda dependencies: - - python=3.7 - - pandas=1.3.3 + - pip + - pip: + - pandas==2.2.3 diff --git a/modules/local/merge_reports/main.nf b/modules/local/merge_reports/main.nf index 70d7d06..616b572 100644 --- a/modules/local/merge_reports/main.nf +++ b/modules/local/merge_reports/main.nf @@ -4,8 +4,8 @@ process MERGE_REPORTS { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-2076f4a3fb468a04063c9e6b7747a630abb457f6:fccb0c41a243c639e11dd1be7b74f563e624fcca-0': - 'biocontainers/mulled-v2-2076f4a3fb468a04063c9e6b7747a630abb457f6:fccb0c41a243c639e11dd1be7b74f563e624fcca-0' }" + 'https://community-cr-prod.seqera.io/docker/registry/v2/blobs/sha256/ab/ab3b0054e3111812d8f2deb12345d5b7ca7ea7b18a2dbcbf174d46274c28deba/data': + 'community.wave.seqera.io/library/pip_pandas:40d2e76c16c136f0' }" input: tuple val(meta), path(inputs) From 7c0b8e69657d3179b47740f7b1e53ba2c15dfb8b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 23 Jan 2025 13:07:29 +0000 Subject: [PATCH 244/292] added new paths to nf-core/test-data --- assets/samplesheet.csv | 5 ++++ assets/samplesheet_full_small.csv | 3 -- assets/samplesheet_full_sv.csv | 3 -- assets/samplesheet_small.csv | 3 -- assets/samplesheet_somatic_indel.csv | 3 -- assets/samplesheet_somatic_snv.csv | 4 --- assets/samplesheet_somatic_sv.csv | 3 -- assets/samplesheet_sv.csv | 4 --- assets/samplesheet_sv_hg37.csv | 3 -- conf/test.config | 8 +++-- conf/test_full.config | 2 +- conf/test_hg37.config | 45 ++++++++++++++++++++++++++++ conf/tests/germline_small.config | 9 +++--- conf/tests/germline_sv.config | 8 ++--- conf/tests/liftover_test.config | 9 +++--- conf/tests/liftover_truth.config | 9 +++--- conf/tests/somatic_indel.config | 11 ++++--- conf/tests/somatic_snv.config | 9 +++--- conf/tests/somatic_sv.config | 6 ++-- 19 files changed, 86 insertions(+), 61 deletions(-) create mode 100644 assets/samplesheet.csv delete mode 100644 assets/samplesheet_full_small.csv delete mode 100644 assets/samplesheet_full_sv.csv delete mode 100644 assets/samplesheet_small.csv delete mode 100644 assets/samplesheet_somatic_indel.csv delete mode 100644 assets/samplesheet_somatic_snv.csv delete mode 100644 assets/samplesheet_somatic_sv.csv delete mode 100644 assets/samplesheet_sv.csv delete mode 100644 assets/samplesheet_sv_hg37.csv create mode 100644 conf/test_hg37.config diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv new file mode 100644 index 0000000..9ed0261 --- /dev/null +++ b/assets/samplesheet.csv @@ -0,0 +1,5 @@ +id,test_vcf,caller +test1,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/HG002_delly_SV_hg19.chr21.vcf.gz,delly +test2,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/HG002_lumpy_SV_hg19.sorted.chr21.vcf.gz,lumpy +test3,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/HG002_manta_SV_hg19_genotype2.chr21.vcf.gz,manta +test4,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/full.svaba.germline.sv.chr21.vcf.gz,svaba diff --git a/assets/samplesheet_full_small.csv b/assets/samplesheet_full_small.csv deleted file mode 100644 index d525fa4..0000000 --- a/assets/samplesheet_full_small.csv +++ /dev/null @@ -1,3 +0,0 @@ -id,test_vcf,caller,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode,subsample -test1,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/SevenBridges_GraphGATKRefine_05052017/HG002-NA24385-50x.union_170414.split.vcf.gz",graph,,,,,,,,,,,,, -test2,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/data/AshkenazimTrio/analysis/OsloUniversityHospital_Exome_GATK_jointVC_11242015/HG002-HG003-HG004.jointVC.filter.vcf",gatk,,,,,,,,,,,,,"Sample_Diag-excap51-HG002-EEogPU" diff --git a/assets/samplesheet_full_sv.csv b/assets/samplesheet_full_sv.csv deleted file mode 100644 index ca2bb05..0000000 --- a/assets/samplesheet_full_sv.csv +++ /dev/null @@ -1,3 +0,0 @@ -id,test_vcf,caller,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,dup_to_ins,pctsize,pctseq,pctovl,evaluationmode,subsample -test1,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/manta_GIABv0.6/diploidSV_PASS_DUPtoINS.vcf.gz",manta,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, -test2,"https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NIST_SV_v0.6/GIAB_Evaluations/BoutrosLab-SV_curation_GIABv0.6/delly_GIABv0.6/norm_recall_HG002.merged__filt_DUPtoINS.vcf.gz",delly,100000,100000,0.3,0.3,0.3,100000,true,true,0.3,0,0,cts, diff --git a/assets/samplesheet_small.csv b/assets/samplesheet_small.csv deleted file mode 100644 index 77934d2..0000000 --- a/assets/samplesheet_small.csv +++ /dev/null @@ -1,3 +0,0 @@ -id,test_vcf,caller -test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.strelka.variants.chr21.vcf.gz,strelka -test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek/hg38/HG002.bcftools.chr21.vcf.gz,bcftools diff --git a/assets/samplesheet_somatic_indel.csv b/assets/samplesheet_somatic_indel.csv deleted file mode 100644 index 7792ae3..0000000 --- a/assets/samplesheet_somatic_indel.csv +++ /dev/null @@ -1,3 +0,0 @@ -id,test_vcf,caller,subsample -test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.freebayes.chr21.vcf.gz,freebayes, -test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_indels.vcf.gz,strelka,"TUMOR" diff --git a/assets/samplesheet_somatic_snv.csv b/assets/samplesheet_somatic_snv.csv deleted file mode 100644 index 07708b9..0000000 --- a/assets/samplesheet_somatic_snv.csv +++ /dev/null @@ -1,4 +0,0 @@ -id,test_vcf,caller,subsample -test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.freebayes.chr21.vcf.gz,freebayes, -test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.manta.somatic_sv.vcf.gz,manta,"HCC1395_HCC1395T" -test3,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.strelka.somatic_snvs.vcf.gz,strelka,"TUMOR" diff --git a/assets/samplesheet_somatic_sv.csv b/assets/samplesheet_somatic_sv.csv deleted file mode 100644 index 7aeae5a..0000000 --- a/assets/samplesheet_somatic_sv.csv +++ /dev/null @@ -1,3 +0,0 @@ -id,test_vcf,caller,subsample -test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.tiddit_sv_merge.vcf.gz,tiddit,"HCC1395_HCC1395T" -test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/sarek_full_test_somatic_v3.4.2/HCC1395T_vs_HCC1395N.manta.somatic_sv.vcf.gz,manta,"HCC1395_HCC1395T" diff --git a/assets/samplesheet_sv.csv b/assets/samplesheet_sv.csv deleted file mode 100644 index dd76036..0000000 --- a/assets/samplesheet_sv.csv +++ /dev/null @@ -1,4 +0,0 @@ -id,test_vcf,caller,pctsize,pctseq,pctovl,refdist,chunksize,normshift,normdist,normsizediff,maxdist,typeignore,evaluationmode -test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/manta.HG002.chr21.vcf.gz,manta,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/Ashkenazim_HG002.filtered.sv.chr21.vcf.gz,merged,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts -test3,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/test/HG002_DRAGEN_SV_hg19.chr21.vcf.gz,dragen,0.3,0,0,100000,100000,0.3,0.3,0.3,100000,true,cts diff --git a/assets/samplesheet_sv_hg37.csv b/assets/samplesheet_sv_hg37.csv deleted file mode 100644 index 2089199..0000000 --- a/assets/samplesheet_sv_hg37.csv +++ /dev/null @@ -1,3 +0,0 @@ -id,test_vcf,caller -test1,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_delly_SV_hg19.chr21.vcf.gz,delly -test2,https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/test/HG002_manta_SV_hg19_genotype.chr21.vcf.gz,manta diff --git a/conf/test.config b/conf/test.config index 4715b0e..14a963b 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,7 +23,8 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_small.csv" + input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_small_germline_hg38.csv" + outdir = "results" // Genome references @@ -38,6 +39,7 @@ params { // truth information truth_id = "HG002" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" + + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/HG002_GRCh38_CMRG_smallvar_v1.00.bed" } diff --git a/conf/test_full.config b/conf/test_full.config index 60a139c..1933f25 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -28,7 +28,7 @@ params { analysis = 'germline' variant_type = "small" method = 'happy,rtgtools' - preprocess = "normalization,deduplication,filter_contigs" + preprocess = "normalize,deduplicate,filter_contigs" truth_id = "HG002" truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.vcf.gz" diff --git a/conf/test_hg37.config b/conf/test_hg37.config new file mode 100644 index 0000000..45b5041 --- /dev/null +++ b/conf/test_hg37.config @@ -0,0 +1,45 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a fast and simple pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test, --outdir + +---------------------------------------------------------------------------------------- +*/ + +process { + resourceLimits = [ + cpus: 4, + memory: '15.GB', + time: '1.h' + ] +} + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Input data + input = "assets/samplesheet.csv" + + outdir = "results" + + // Genome references + genome = 'GRCh37' + + // Processes + analysis = 'germline' + variant_type = "structural" + method = 'truvari,svanalyzer' + preprocess = "normalize,deduplicate,filter_contigs" + + + // truth information + truth_id = "HG002" + + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" +} diff --git a/conf/tests/germline_small.config b/conf/tests/germline_small.config index 1644408..9b98222 100644 --- a/conf/tests/germline_small.config +++ b/conf/tests/germline_small.config @@ -23,7 +23,7 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_small.csv" + input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_small_germline_hg38.csv" outdir = "results" // Genome references @@ -38,8 +38,7 @@ params { // truth information truth_id = "HG002" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/CRMG_benchmark_dataset/HG002_GRCh38_CMRG_smallvar_v1.00.bed" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" - + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_CMRG_smallvar_v1.00.bed" + rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config index 18d2729..5128134 100644 --- a/conf/tests/germline_sv.config +++ b/conf/tests/germline_sv.config @@ -23,7 +23,7 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_sv.csv" + input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_sv_germline_hg38.csv" outdir = "results" // Genome references @@ -39,7 +39,7 @@ params { truth_id = "HG002" include_expression = 'FILTER="."' - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/liftover_test.config b/conf/tests/liftover_test.config index f3878e2..007b542 100644 --- a/conf/tests/liftover_test.config +++ b/conf/tests/liftover_test.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_sv_liftover.csv" + input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_sv_germline_hg37_liftover.csv" outdir = 'results' genome = 'GRCh37' @@ -32,11 +32,12 @@ params { min_sv_size = 30 truth_id = "HG002" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.bed" //liftover files liftover = "test" chain = "http://ftp.ensembl.org/pub/assembly_mapping/homo_sapiens/GRCh38_to_GRCh37.chain.gz" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch38_grch37.txt" + rename_chr = "${projectDir}/assets/rename_contigs/grch38_grch37.txt" + } diff --git a/conf/tests/liftover_truth.config b/conf/tests/liftover_truth.config index e16bd88..0c5b6ab 100644 --- a/conf/tests/liftover_truth.config +++ b/conf/tests/liftover_truth.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_small.csv' + input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_small_germline_hg38.csv" outdir = 'results' genome = 'GRCh38' @@ -31,11 +31,12 @@ params { method = 'rtgtools,happy' preprocess = "split_multiallelic,deduplicate,filter_contigs,normalize" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_GRCh37_1_22_v4.2.1_highconf.bed" + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg37/truth/germline/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg37/truth/germline/HG002_GRCh37_1_22_v4.2.1_highconf.bed" //liftover files liftover = "truth" chain = "https://raw.githubusercontent.com/broadinstitute/gatk/master/scripts/funcotator/data_sources/gnomAD/b37ToHg38.over.chain" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" + } diff --git a/conf/tests/somatic_indel.config b/conf/tests/somatic_indel.config index 67ffe08..76cf857 100644 --- a/conf/tests/somatic_indel.config +++ b/conf/tests/somatic_indel.config @@ -20,7 +20,8 @@ params { max_time = '8.h' // Input data - input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_somatic_indel.csv' + input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_indel_somatic_hg38.csv" + outdir = 'results' // Genome references @@ -31,9 +32,7 @@ params { preprocess = "filter_contigs" variant_type = "indel" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sINDEL_truth_set_v1.0.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" - - + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg38/truth/somatic/sINDEL_truth_set_v1.0.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg38/truth/somatic/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" + rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/somatic_snv.config b/conf/tests/somatic_snv.config index 83a92f2..5327703 100644 --- a/conf/tests/somatic_snv.config +++ b/conf/tests/somatic_snv.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_somatic_snv.csv' + input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_snv_somatic_hg38.csv" outdir = 'results' // Genome references @@ -31,8 +31,7 @@ params { variant_type = "snv" truth_id = "SEQC2" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/sSNV_truth_set_v1.0.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" - + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg38/truth/somatic/sSNV_truth_set_v1.0.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg38/truth/somatic/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" + rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/somatic_sv.config b/conf/tests/somatic_sv.config index 00e2151..18508b6 100644 --- a/conf/tests/somatic_sv.config +++ b/conf/tests/somatic_sv.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = 'https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/samplesheet_somatic_sv.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_sv_somatic_hg38.csv' outdir = 'results' // Genome references @@ -31,7 +31,7 @@ params { preprocess = "filter_contigs" truth_id = "SEQC2" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/somatic/truth/hg38/13059_2022_2816_MOESM4_ESM.vcf.gz" - rename_chr = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/grch37_grch38.txt" + truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/hg38/truth/somatic/13059_2022_2816_MOESM4_ESM.vcf.gz" + rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } From 009b72e596595f54f238250fa80b6fb839a80a64 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 24 Jan 2025 08:57:08 +0000 Subject: [PATCH 245/292] update snapshots --- conf/modules.config | 2 +- conf/tests/liftover_test.config | 4 ++-- tests/germline_small.nf.test.snap | 12 ++++++------ tests/germline_sv.nf.test.snap | 12 ++++++------ tests/liftover_test.nf.test.snap | 15 +++++++-------- tests/liftover_truth.nf.test.snap | 6 +++--- tests/somatic_indel.nf.test.snap | 6 +++--- tests/somatic_snv.nf.test.snap | 6 +++--- tests/somatic_sv.nf.test.snap | 6 +++--- 9 files changed, 34 insertions(+), 35 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 9c9888d..5b834e3 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -97,7 +97,7 @@ process { ext.prefix = {input.baseName - ".vcf" + ".renamechr"} ext.args = {"--output-type z"} publishDir = [ - path: {params.liftover == "truth"? "${params.outdir}/${params.variant_type}/${params.truth_id}/preprocess/liftover" : "${params.outdir}/${params.variant_type}/${meta.id}/preporcess/liftover"}, + path: {params.liftover == "truth"? "${params.outdir}/${params.variant_type}/${params.truth_id}/preprocess/liftover" : "${params.outdir}/${params.variant_type}/${meta.id}/preprocess/liftover"}, pattern: "*{.vcf.gz}", mode: params.publish_dir_mode ] diff --git a/conf/tests/liftover_test.config b/conf/tests/liftover_test.config index f3878e2..399e104 100644 --- a/conf/tests/liftover_test.config +++ b/conf/tests/liftover_test.config @@ -32,8 +32,8 @@ params { min_sv_size = 30 truth_id = "HG002" - truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg38/truth/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + truth_vcf = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" + regions_bed = "https://raw.githubusercontent.com/kubranarci/benchmark_datasets/main/SV_testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" //liftover files liftover = "test" diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 6e64de8..41eb52f 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -49,7 +49,7 @@ "pre.py": "0.3.14" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -242,9 +242,9 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-16T15:55:45.473583366" + "timestamp": "2025-01-23T14:25:38.877918008" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ @@ -296,7 +296,7 @@ "pre.py": "0.3.14" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -599,8 +599,8 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-17T09:31:09.115891055" + "timestamp": "2025-01-23T14:23:40.862874525" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index 63b16d5..c56fade 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -46,7 +46,7 @@ "datavzrd": "2.36.12" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -360,9 +360,9 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-17T09:36:16.512872909" + "timestamp": "2025-01-23T14:44:34.135136556" }, "-stub": { "content": [ @@ -411,7 +411,7 @@ "datavzrd": "2.36.12" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -664,8 +664,8 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-16T15:43:53.130315988" + "timestamp": "2025-01-23T14:46:46.270536815" } } \ No newline at end of file diff --git a/tests/liftover_test.nf.test.snap b/tests/liftover_test.nf.test.snap index 44a77ed..7a2f9c5 100644 --- a/tests/liftover_test.nf.test.snap +++ b/tests/liftover_test.nf.test.snap @@ -34,7 +34,7 @@ "datavzrd": "2.36.12" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PICARD_CREATESEQUENCEDICTIONARY": { "picard": "3.2.0-1-g3948afb6b" @@ -196,10 +196,9 @@ "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-base.vcf.gz.tbi", "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz", "structural/test2/benchmarks/truvari/test2.HG002.manta.tp-comp.vcf.gz.tbi", - "structural/test2/preporcess", - "structural/test2/preporcess/liftover", - "structural/test2/preporcess/liftover/test2.reformatted.renamechr.vcf.gz", "structural/test2/preprocess", + "structural/test2/preprocess/liftover", + "structural/test2/preprocess/liftover/test2.reformatted.renamechr.vcf.gz", "structural/test2/preprocess/test2.vcf.gz", "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", @@ -209,8 +208,8 @@ "structural/test2/stats/survivor/test2.manta_mqc.stats" ], [ - "HG002.bcftools_stats.txt:md5,142c1fab782c9eb2aa932440e1589461", - "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", + "HG002.bcftools_stats.txt:md5,f63d8da43be98bae4e02c43966313e5c", + "HG002_mqc.stats:md5,ba6a6398a99b95579872576cacde4c8c", "test1.delly.bcftools_stats.txt:md5,f204ab10d82b3cbc9ee51cb6ad548121", "test1.delly_mqc.stats:md5,e140ad55975c767578b0dd6aff58ba29", "test2.manta.bcftools_stats.txt:md5,0de1446278a5448b7dc5a02bcbf927a4", @@ -219,8 +218,8 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-17T10:22:18.275352814" + "timestamp": "2025-01-24T08:56:44.966454325" } } \ No newline at end of file diff --git a/tests/liftover_truth.nf.test.snap b/tests/liftover_truth.nf.test.snap index 98508cc..fce9df1 100644 --- a/tests/liftover_truth.nf.test.snap +++ b/tests/liftover_truth.nf.test.snap @@ -49,7 +49,7 @@ "hap.py": "0.3.14" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -361,8 +361,8 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-17T09:02:13.833941457" + "timestamp": "2025-01-24T08:45:10.41490994" } } \ No newline at end of file diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index a446e99..2e11e13 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -31,7 +31,7 @@ "hap.py": "0.3.14" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -179,8 +179,8 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-17T09:20:01.232442793" + "timestamp": "2025-01-23T15:22:31.866027222" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 765e3f6..fafc685 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -31,7 +31,7 @@ "hap.py": "0.3.14" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -130,8 +130,8 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-17T09:17:06.41202116" + "timestamp": "2025-01-23T15:15:47.829389807" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index c495cc5..ffadf6b 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -43,7 +43,7 @@ "datavzrd": "2.36.12" }, "MERGE_REPORTS": { - "python": "3.8.6" + "python": "3.13.0" }, "PLOTS": { "r-base": "4.3.1" @@ -216,8 +216,8 @@ ], "meta": { "nf-test": "0.9.2", - "nextflow": "24.10.3" + "nextflow": "24.10.4" }, - "timestamp": "2025-01-17T09:27:15.598502393" + "timestamp": "2025-01-23T15:08:15.346103131" } } \ No newline at end of file From 15a980f40760482919b3156dd80dcd75256fb3c1 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 27 Jan 2025 14:49:32 +0000 Subject: [PATCH 246/292] Template update for nf-core/tools version 3.2.0 --- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 33 ------------------- .nf-core.yml | 2 +- .pre-commit-config.yaml | 2 +- docs/output.md | 11 +++++-- modules.json | 4 +-- modules/nf-core/fastqc/main.nf | 20 +++++------ modules/nf-core/fastqc/tests/tags.yml | 2 -- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +-- .../nf-core/multiqc/tests/main.nf.test.snap | 24 +++++++------- nextflow.config | 16 ++++----- ro-crate-metadata.json | 12 +++---- 13 files changed, 52 insertions(+), 82 deletions(-) delete mode 100644 modules/nf-core/fastqc/tests/tags.yml diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bed96d..95b6b6a 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@80620a5d27ce0ae443b965134db88467fc607b43 # v7 + uses: dawidd6/action-download-artifact@20319c5641d495c8a52e688b7dc5fada6c3a9fbc # v8 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 450b1d5..76a9e67 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -27,39 +27,6 @@ jobs: ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics - send-tweet: - runs-on: ubuntu-latest - - steps: - - uses: actions/setup-python@0b93645e9fea7318ecaed2b359559ac225c90a2b # v5 - with: - python-version: "3.10" - - name: Install dependencies - run: pip install tweepy==4.14.0 - - name: Send tweet - shell: python - run: | - import os - import tweepy - - client = tweepy.Client( - access_token=os.getenv("TWITTER_ACCESS_TOKEN"), - access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), - consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), - consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), - ) - tweet = os.getenv("TWEET") - client.create_tweet(text=tweet) - env: - TWEET: | - Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! - - Please see the changelog: ${{ github.event.release.html_url }} - TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} - TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} - TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} - TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} - bsky-post: runs-on: ubuntu-latest steps: diff --git a/.nf-core.yml b/.nf-core.yml index 6f583e4..895986f 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,6 +1,6 @@ lint: actions_ci: false -nf_core_version: 3.1.2 +nf_core_version: 3.2.0 repository_type: pipeline template: author: kuebra.narci@dkfz.de diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 9e9f0e1..1dec865 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -7,7 +7,7 @@ repos: - prettier@3.2.5 - repo: https://github.com/editorconfig-checker/editorconfig-checker.python - rev: "3.0.3" + rev: "3.1.2" hooks: - id: editorconfig-checker alias: ec diff --git a/docs/output.md b/docs/output.md index 228b547..eb14937 100644 --- a/docs/output.md +++ b/docs/output.md @@ -12,7 +12,8 @@ The directories listed below will be created in the results directory after the The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps: -- [FastQC](#fastqc) - Raw read QC- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline +- [FastQC](#fastqc) - Raw read QC +- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline - [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution ### FastQC @@ -26,7 +27,9 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
    -[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).### MultiQC +[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/). + +### MultiQC
    Output files @@ -40,7 +43,9 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d [MultiQC](http://multiqc.info) is a visualization tool that generates a single HTML report summarising all samples in your project. Most of the pipeline QC results are visualised in the report and further statistics are available in the report data directory. -Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see .### Pipeline information +Results generated by MultiQC collate pipeline QC from supported tools e.g. FastQC. The pipeline has special steps which also allow the software versions to be reported in the MultiQC output for future traceability. For more information about how to use MultiQC reports, see . + +### Pipeline information
    Output files diff --git a/modules.json b/modules.json index ce29ad9..a5306eb 100644 --- a/modules.json +++ b/modules.json @@ -7,12 +7,12 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "dc94b6ee04a05ddb9f7ae050712ff30a13149164", + "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d", + "git_sha": "f0719ae309075ae4a291533883847c3f7c441dad", "installed_by": ["modules"] } } diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 752c3a1..033f415 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -1,5 +1,5 @@ process FASTQC { - tag "$meta.id" + tag "${meta.id}" label 'process_medium' conda "${moduleDir}/environment.yml" @@ -19,30 +19,30 @@ process FASTQC { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" // Make list of old name and new name pairs to use for renaming in the bash while loop def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } - def rename_to = old_new_pairs*.join(' ').join(' ') + def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ _old_name, new_name -> new_name }.join(' ') // The total amount of allocated RAM by FastQC is equal to the number of threads defined (--threads) time the amount of RAM defined (--memory) // https://github.com/s-andrews/FastQC/blob/1faeea0412093224d7f6a07f777fad60a5650795/fastqc#L211-L222 // Dividing the task.memory by task.cpu allows to stick to requested amount of RAM in the label - def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') / task.cpus + def memory_in_mb = task.memory ? task.memory.toUnit('MB').toFloat() / task.cpus : null // FastQC memory value allowed range (100 - 10000) def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) """ - printf "%s %s\\n" $rename_to | while read old_name new_name; do + printf "%s %s\\n" ${rename_to} | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name done fastqc \\ - $args \\ - --threads $task.cpus \\ - --memory $fastqc_memory \\ - $renamed_files + ${args} \\ + --threads ${task.cpus} \\ + --memory ${fastqc_memory} \\ + ${renamed_files} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml deleted file mode 100644 index 7834294..0000000 --- a/modules/nf-core/fastqc/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -fastqc: - - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 6f5b867..a27122c 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -2,4 +2,4 @@ channels: - conda-forge - bioconda dependencies: - - bioconda::multiqc=1.25.1 + - bioconda::multiqc=1.27 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index cc0643e..58d9313 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.25.1--pyhdfd78af_0' : - 'biocontainers/multiqc:1.25.1--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.27--pyhdfd78af_0' : + 'biocontainers/multiqc:1.27--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index 2fcbb5f..7b7c132 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" + "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.4" + "nf-test": "0.9.2", + "nextflow": "24.10.4" }, - "timestamp": "2024-10-02T17:51:46.317523" + "timestamp": "2025-01-27T09:29:57.631982377" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" + "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.4" + "nf-test": "0.9.2", + "nextflow": "24.10.4" }, - "timestamp": "2024-10-02T17:52:20.680978" + "timestamp": "2025-01-27T09:30:34.743726958" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,41f391dcedce7f93ca188f3a3ffa0916" + "versions.yml:md5,8f3b8c1cec5388cf2708be948c9fa42f" ] ], "meta": { - "nf-test": "0.9.0", - "nextflow": "24.04.4" + "nf-test": "0.9.2", + "nextflow": "24.10.4" }, - "timestamp": "2024-10-02T17:52:09.185842" + "timestamp": "2025-01-27T09:30:21.44383553" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 53b78d2..2e69080 100644 --- a/nextflow.config +++ b/nextflow.config @@ -195,14 +195,14 @@ env { } // Set bash options -process.shell = """\ -bash - -set -e # Exit if a tool returns a non-zero status/exit code -set -u # Treat unset variables and parameters as an error -set -o pipefail # Returns the status of the last command to exit with a non-zero status or zero if all successfully execute -set -C # No clobber - prevent output redirection from overwriting files. -""" +process.shell = [ + "bash", + "-C", // No clobber - prevent output redirection from overwriting files. + "-e", // Exit if a tool returns a non-zero status/exit code + "-u", // Treat unset variables and parameters as an error + "-o", // Returns the status of the last command to exit.. + "pipefail" // ..with a non-zero status or zero if all successfully execute +] // Disable process selector warnings by default. Use debug profile to enable warnings. nextflow.enable.configProcessNamesValidation = false diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json index db7990a..4d6651d 100644 --- a/ro-crate-metadata.json +++ b/ro-crate-metadata.json @@ -22,7 +22,7 @@ "@id": "./", "@type": "Dataset", "creativeWorkStatus": "InProgress", - "datePublished": "2025-01-20T14:36:33+00:00", + "datePublished": "2025-01-27T14:49:17+00:00", "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    \n\n[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is a bioinformatics pipeline that ...\n\n\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n\n\nNow, you can run the pipeline using:\n\n\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", "hasPart": [ { @@ -99,7 +99,7 @@ }, "mentions": [ { - "@id": "#6051d38b-d786-4206-bf51-525cae6a07df" + "@id": "#ae45bd39-5f9e-4b26-b5c0-9ca6bc1fa200" } ], "name": "nf-core/variantbenchmarking" @@ -123,7 +123,7 @@ "@id": "main.nf", "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"], "dateCreated": "", - "dateModified": "2025-01-20T14:36:33Z", + "dateModified": "2025-01-27T14:49:17Z", "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/", "keywords": ["nf-core", "nextflow", "benchmark", "draft", "structural-variants", "variant-calling"], "license": ["MIT"], @@ -150,11 +150,11 @@ "version": "!>=24.04.2" }, { - "@id": "#6051d38b-d786-4206-bf51-525cae6a07df", + "@id": "#ae45bd39-5f9e-4b26-b5c0-9ca6bc1fa200", "@type": "TestSuite", "instance": [ { - "@id": "#df38e5e1-380b-45f5-978b-955d38f83885" + "@id": "#5b391f3e-435e-4c5f-8beb-cb6dc0854d4f" } ], "mainEntity": { @@ -163,7 +163,7 @@ "name": "Test suite for nf-core/variantbenchmarking" }, { - "@id": "#df38e5e1-380b-45f5-978b-955d38f83885", + "@id": "#5b391f3e-435e-4c5f-8beb-cb6dc0854d4f", "@type": "TestInstance", "name": "GitHub Actions workflow for testing nf-core/variantbenchmarking", "resource": "repos/nf-core/variantbenchmarking/actions/workflows/ci.yml", From a95b1231f3a39a3c31bbd6528170701b6e094c57 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 29 Jan 2025 10:33:43 +0000 Subject: [PATCH 247/292] resolve conflicts --- nextflow.config | 4 ---- 1 file changed, 4 deletions(-) diff --git a/nextflow.config b/nextflow.config index 2967af1..492c261 100644 --- a/nextflow.config +++ b/nextflow.config @@ -297,12 +297,8 @@ manifest { // Nextflow plugins plugins { -<<<<<<< HEAD id 'nf-schema@2.3.0' // Validation of pipeline parameters and creation of an input channel from a sample sheet -======= - id 'nf-schema@2.1.1' // Validation of pipeline parameters and creation of an input channel from a sample sheet id 'nf-prov@1.2.2' // Provenance reports for pipeline runs ->>>>>>> dev } validation { From ebdf4432a52da2017aa6f56acea767c3493fd7e3 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 29 Jan 2025 10:36:31 +0000 Subject: [PATCH 248/292] remove bcftools_query --- modules.json | 5 ----- 1 file changed, 5 deletions(-) diff --git a/modules.json b/modules.json index 47e9306..faee1cb 100644 --- a/modules.json +++ b/modules.json @@ -25,11 +25,6 @@ "git_sha": "08108058ea36a63f141c25c4e75f9f872a5b2296", "installed_by": ["modules"] }, - "bcftools/query": { - "branch": "master", - "git_sha": "a5ba4d59c2b248c0379b0f8aeb4e7e754566cd1f", - "installed_by": ["modules"] - }, "bcftools/reheader": { "branch": "master", "git_sha": "666652151335353eef2fcd58880bcef5bc2928e1", From 8058e248c82a4d1d278267c6c719c74410807d0a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 29 Jan 2025 10:43:16 +0000 Subject: [PATCH 249/292] gitignore --- .nf-core.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 1491692..c398cf4 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,5 +1,7 @@ lint: actions_ci: false + files_unchanged: + - .gitignore nf_core_version: 3.2.0 repository_type: pipeline template: From b3eb22ef04fe0d468d2c6ca94ee18ff9ef8b57f2 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 29 Jan 2025 10:52:54 +0000 Subject: [PATCH 250/292] fix trace_timestamp --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 492c261..253ba63 100644 --- a/nextflow.config +++ b/nextflow.config @@ -267,7 +267,7 @@ prov { enabled = true formats { bco { - file = "${params.outdir}/pipeline_info/manifest_${trace_timestamp}.bco.json" + file = "${params.outdir}/pipeline_info/manifest_${params.trace_timestamp}.bco.json" } } } From 2a953d917cbf0840a3707e7f0358e28dc738e588 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 29 Jan 2025 10:59:23 +0000 Subject: [PATCH 251/292] fix attempt trace_report_suffix --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 253ba63..200178a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -267,7 +267,7 @@ prov { enabled = true formats { bco { - file = "${params.outdir}/pipeline_info/manifest_${params.trace_timestamp}.bco.json" + file = "${params.outdir}/pipeline_info/manifest_${params.trace_report_suffix}.bco.json" } } } From 2fba8137dc48418070e4d5369369543826fbafcc Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 29 Jan 2025 11:05:13 +0000 Subject: [PATCH 252/292] missing conflicts --- workflows/variantbenchmarking.nf | 22 ---------------------- 1 file changed, 22 deletions(-) diff --git a/workflows/variantbenchmarking.nf b/workflows/variantbenchmarking.nf index 7662499..7bd1558 100644 --- a/workflows/variantbenchmarking.nf +++ b/workflows/variantbenchmarking.nf @@ -284,28 +284,6 @@ workflow VARIANTBENCHMARKING { ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml',sort: true)) ch_multiqc_files = ch_multiqc_files.mix(REPORT_BENCHMARK_STATISTICS.out.ch_plots) -<<<<<<< HEAD - summary_params = paramsSummaryMap( - workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - ch_multiqc_files = ch_multiqc_files.mix( - ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? - file(params.multiqc_methods_description, checkIfExists: true) : - file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value( - methodsDescriptionText(ch_multiqc_custom_methods_description)) - - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix( - ch_methods_description.collectFile( - name: 'methods_description_mqc.yaml', - sort: true - ) - ) -======= ->>>>>>> dev - MULTIQC ( ch_multiqc_files.collect(), ch_multiqc_config.toList(), From 6d0027dcb8e93c47e9570852b19fde7f7f5b3b77 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 29 Jan 2025 12:41:28 +0000 Subject: [PATCH 253/292] renew snapshots --- tests/germline_small.nf.test.snap | 146 +----------------------- tests/germline_sv.nf.test.snap | 182 +----------------------------- tests/liftover_test.nf.test.snap | 67 +---------- tests/liftover_truth.nf.test.snap | 76 +------------ tests/somatic_indel.nf.test.snap | 43 +------ tests/somatic_snv.nf.test.snap | 43 +------ tests/somatic_sv.nf.test.snap | 70 +----------- 7 files changed, 27 insertions(+), 600 deletions(-) diff --git a/tests/germline_small.nf.test.snap b/tests/germline_small.nf.test.snap index 41eb52f..4790f69 100644 --- a/tests/germline_small.nf.test.snap +++ b/tests/germline_small.nf.test.snap @@ -2,77 +2,10 @@ "-stub": { "content": [ 62, - { - "BCFTOOLS_DEDUP": { - "bcftools": 1.2 - }, - "BCFTOOLS_FILTER": { - "bcftools": 1.2 - }, - "BCFTOOLS_MERGE": { - "bcftools": 1.2 - }, - "BCFTOOLS_NORM": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_1": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_2": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_3": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_4": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "HAPPY_HAPPY": { - "hap.py": "0.3.14" - }, - "HAPPY_PREPY": { - "pre.py": "0.3.14" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "REFORMAT_HEADER": { - "tabix": 1.12 - }, - "RTGTOOLS_FORMAT": { - "rtg-tools": "3.12.1" - }, - "RTGTOOLS_VCFEVAL": { - "rtg-tools": "3.12.1" - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "references", "references/rtgtools", "references/rtgtools/genome.sdf", @@ -244,82 +177,15 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-23T14:25:38.877918008" + "timestamp": "2025-01-29T12:14:19.21651889" }, "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools'": { "content": [ 62, - { - "BCFTOOLS_DEDUP": { - "bcftools": 1.2 - }, - "BCFTOOLS_FILTER": { - "bcftools": 1.2 - }, - "BCFTOOLS_MERGE": { - "bcftools": 1.2 - }, - "BCFTOOLS_NORM": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_1": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_2": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_3": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_4": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "HAPPY_HAPPY": { - "hap.py": "0.3.14" - }, - "HAPPY_PREPY": { - "pre.py": "0.3.14" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "REFORMAT_HEADER": { - "tabix": 1.12 - }, - "RTGTOOLS_FORMAT": { - "rtg-tools": "3.12.1" - }, - "RTGTOOLS_VCFEVAL": { - "rtg-tools": "3.12.1" - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "references", "references/rtgtools", "references/rtgtools/genome.sdf", @@ -601,6 +467,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-23T14:23:40.862874525" + "timestamp": "2025-01-29T12:12:18.418200369" } } \ No newline at end of file diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index c56fade..b854110 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -2,95 +2,10 @@ "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'": { "content": [ 121, - { - "BCFTOOLS_DEDUP": { - "bcftools": 1.2 - }, - "BCFTOOLS_FILTER": { - "bcftools": 1.2 - }, - "BCFTOOLS_NORM": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_1": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_2": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_3": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_4": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.2 - }, - "BGZIP_TABIX": { - "tabix": 1.12 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "SURVIVOR_MERGE": { - "survivor": "1.0.7" - }, - "SURVIVOR_STATS": { - "survivor": "1.0.7" - }, - "SVANALYZER_SVBENCHMARK": { - "svanalyzer": 0.36 - }, - "SVYNC": { - "svync": "0.1.2" - }, - "TABIX_BGZIP_QUERY": { - "tabix": "1.19.1" - }, - "TABIX_BGZIP_TRUTH": { - "tabix": "1.19.1" - }, - "TABIX_BGZIP_UNZIP": { - "tabix": "1.19.1" - }, - "TRUVARI_BENCH": { - "truvari": "4.1.0)" - }, - "VARIANT_EXTRACTOR": { - "python": "3.12.4" - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "WITTYER": { - "wittyer": "0.5.2.0" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "structural", "structural/HG002", "structural/HG002/preprocess", @@ -362,100 +277,15 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-23T14:44:34.135136556" + "timestamp": "2025-01-29T12:17:47.644313415" }, "-stub": { "content": [ 121, - { - "BCFTOOLS_DEDUP": { - "bcftools": 1.2 - }, - "BCFTOOLS_FILTER": { - "bcftools": 1.2 - }, - "BCFTOOLS_NORM": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_1": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_2": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_3": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_4": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.2 - }, - "BGZIP_TABIX": { - "tabix": 1.12 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "SURVIVOR_MERGE": { - "survivor": "1.0.7" - }, - "SURVIVOR_STATS": { - "survivor": "1.0.7" - }, - "SVANALYZER_SVBENCHMARK": { - "svanalyzer": 0.36 - }, - "SVYNC": { - "svync": "0.1.2" - }, - "TABIX_BGZIP_QUERY": { - "tabix": "1.19.1" - }, - "TABIX_BGZIP_TRUTH": { - "tabix": "1.19.1" - }, - "TABIX_BGZIP_UNZIP": { - "tabix": "1.19.1" - }, - "TRUVARI_BENCH": { - "truvari": "4.1.0)" - }, - "VARIANT_EXTRACTOR": { - "python": "3.12.4" - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "WITTYER": { - "wittyer": "0.5.2.0" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "structural", "structural/HG002", "structural/HG002/preprocess", @@ -666,6 +496,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-23T14:46:46.270536815" + "timestamp": "2025-01-29T12:20:02.006578062" } } \ No newline at end of file diff --git a/tests/liftover_test.nf.test.snap b/tests/liftover_test.nf.test.snap index 7a2f9c5..ca7620b 100644 --- a/tests/liftover_test.nf.test.snap +++ b/tests/liftover_test.nf.test.snap @@ -2,71 +2,10 @@ "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari' --liftover 'test'": { "content": [ 57, - { - "BCFTOOLS_REHEADER_1": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_2": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_3": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_4": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.2 - }, - "BGZIP_TABIX": { - "tabix": 1.12 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PICARD_CREATESEQUENCEDICTIONARY": { - "picard": "3.2.0-1-g3948afb6b" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "SURVIVOR_MERGE": { - "survivor": "1.0.7" - }, - "SURVIVOR_STATS": { - "survivor": "1.0.7" - }, - "TABIX_BGZIP": { - "tabix": "1.19.1" - }, - "TABIX_BGZIP_UNZIP": { - "tabix": "1.19.1" - }, - "TRUVARI_BENCH": { - "truvari": "4.1.0)" - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "references", "references/dictionary", "references/dictionary/genome.dict", @@ -220,6 +159,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-24T08:56:44.966454325" + "timestamp": "2025-01-29T12:23:50.918953841" } } \ No newline at end of file diff --git a/tests/liftover_truth.nf.test.snap b/tests/liftover_truth.nf.test.snap index fce9df1..2e19c0f 100644 --- a/tests/liftover_truth.nf.test.snap +++ b/tests/liftover_truth.nf.test.snap @@ -2,80 +2,10 @@ "Params: --analysis 'germline' --variant_type 'small' --method 'happy,rtgtools' --liftover 'truth'": { "content": [ 68, - { - "BCFTOOLS_DEDUP": { - "bcftools": 1.2 - }, - "BCFTOOLS_MERGE": { - "bcftools": 1.2 - }, - "BCFTOOLS_NORM": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_1": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_2": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_3": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_4": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_SPLIT_MULTI": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.2 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "HAPPY_HAPPY": { - "hap.py": "0.3.14" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "REFORMAT_HEADER": { - "tabix": 1.12 - }, - "RTGTOOLS_FORMAT": { - "rtg-tools": "3.12.1" - }, - "RTGTOOLS_VCFEVAL": { - "rtg-tools": "3.12.1" - }, - "UCSC_LIFTOVER": { - "ucsc": 377 - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "references", "references/dictionary", "references/dictionary/genome.dict", @@ -363,6 +293,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-24T08:45:10.41490994" + "timestamp": "2025-01-29T12:28:06.56131727" } } \ No newline at end of file diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index 2e11e13..408df18 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -2,44 +2,7 @@ "Params: --analysis 'somatic' --variant_type 'indel' --method 'sompy'": { "content": [ 21, - { - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.2 - }, - "BCFTOOLS_VIEW_FILTERMISSING": { - "bcftools": 1.2 - }, - "BCFTOOLS_VIEW_SUBSAMPLE": { - "bcftools": 1.2 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "HAPPY_SOMPY": { - "hap.py": "0.3.14" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "indel", "indel/SEQC2", @@ -169,7 +132,7 @@ "indel/test2/stats/bcftools", "indel/test2/stats/bcftools/test2.strelka.bcftools_stats.txt", "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml" + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml" ], [ "SEQC2.bcftools_stats.txt:md5,97beec3b6ce6471625018d91c75df08d", @@ -181,6 +144,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-23T15:22:31.866027222" + "timestamp": "2025-01-29T12:30:05.839484004" } } \ No newline at end of file diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index fafc685..1426953 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -2,47 +2,10 @@ "-stub": { "content": [ 29, - { - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.2 - }, - "BCFTOOLS_VIEW_FILTERMISSING": { - "bcftools": 1.2 - }, - "BCFTOOLS_VIEW_SUBSAMPLE": { - "bcftools": 1.2 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "HAPPY_SOMPY": { - "hap.py": "0.3.14" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "snv", "snv/SEQC2", "snv/SEQC2/preprocess", @@ -132,6 +95,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-23T15:15:47.829389807" + "timestamp": "2025-01-29T12:33:52.837161011" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index ffadf6b..f061062 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -2,74 +2,10 @@ "Params: --analysis 'somatic' --variant_type 'structural' --method 'truvari,svbenchmark'": { "content": [ 53, - { - "BCFTOOLS_REHEADER_1": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_2": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_3": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_4": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_QUERY": { - "bcftools": 1.2 - }, - "BCFTOOLS_REHEADER_TRUTH": { - "bcftools": 1.2 - }, - "BCFTOOLS_SORT": { - "bcftools": 1.2 - }, - "BCFTOOLS_STATS": { - "bcftools": 1.18 - }, - "BCFTOOLS_VIEW_CONTIGS": { - "bcftools": 1.2 - }, - "BCFTOOLS_VIEW_FILTERMISSING": { - "bcftools": 1.2 - }, - "BCFTOOLS_VIEW_SUBSAMPLE": { - "bcftools": 1.2 - }, - "BGZIP_TABIX": { - "tabix": 1.12 - }, - "DATAVZRD": { - "datavzrd": "2.36.12" - }, - "MERGE_REPORTS": { - "python": "3.13.0" - }, - "PLOTS": { - "r-base": "4.3.1" - }, - "SURVIVOR_MERGE": { - "survivor": "1.0.7" - }, - "SURVIVOR_STATS": { - "survivor": "1.0.7" - }, - "TABIX_BGZIP_UNZIP": { - "tabix": "1.19.1" - }, - "TRUVARI_BENCH": { - "truvari": "4.1.0)" - }, - "VCF_TO_CSV": { - "python": "3.12.4" - }, - "Workflow": { - "nf-core/variantbenchmarking": "v1.0dev" - } - }, + null, [ "pipeline_info", - "pipeline_info/nf_core_pipeline_software_mqc_versions.yml", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", "structural", "structural/SEQC2", "structural/SEQC2/preprocess", @@ -218,6 +154,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-23T15:08:15.346103131" + "timestamp": "2025-01-29T12:35:54.329231974" } } \ No newline at end of file From f85cbb86d3a60cf704da292cfa38e84039e0893d Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 10:49:47 +0000 Subject: [PATCH 254/292] add citations --- CITATIONS.md | 58 ++++++++++++++++++++++++++++++++++++++++++++++++++-- 1 file changed, 56 insertions(+), 2 deletions(-) diff --git a/CITATIONS.md b/CITATIONS.md index 44ca0a0..c37ef6f 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -10,14 +10,68 @@ ## Pipeline tools -- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) +- [Bcftools](http://samtools.github.io/bcftools/bcftools.html) -> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. +> Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. doi: 10.1093/bioinformatics/btp352. Epub 2009 Jun 8. PMID: 19505943; PMCID: PMC2723002. + +- [BEDTools](https://bedtools.readthedocs.io/) + +> Aaron R. Quinlan, Ira M. Hall, BEDTools: a flexible suite of utilities for comparing genomic features, Bioinformatics, Volume 26, Issue 6, March 2010, Pages 841–842, https://doi.org/10.1093/bioinformatics/btq033 + +- [datavzrd](https://datavzrd.github.io/docs/index.html) + +- [hap.py](https://www.illumina.com/products/by-type/informatics-products/basespace-sequence-hub/apps/hap-py-benchmarking.html) + +- [manta](https://github.com/Illumina/manta/blob/v1.6.0/docs/userGuide/README.md) + +> Xiaoyu Chen, Ole Schulz-Trieglaff, Richard Shaw, Bret Barnes, Felix Schlesinger, Morten Källberg, Anthony J. Cox, Semyon Kruglyak, Christopher T. Saunders, Manta: rapid detection of structural variants and indels for germline and cancer sequencing applications, Bioinformatics, Volume 32, Issue 8, April 2016, Pages 1220–1222, https://doi.org/10.1093/bioinformatics/btv710 - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. +- [picard](https://gatk.broadinstitute.org/hc/en-us/articles/360036712531-CreateSequenceDictionary-Picard) + +- [RTG Tools](https://www.realtimegenomics.com/products/rtg-tools) + +> John G. Cleary, Ross Braithwaite, Kurt Gaastra, Brian S. Hilbush, Stuart Inglis, Sean A. Irvine, Alan Jackson, Richard Littin, Mehul Rathod, David Ware, Justin M. Zook, Len Trigg, Francisco M. De La Vega bioRxiv 023754; doi: https://doi.org/10.1101/023754 + +- [SURVIVOR](https://github.com/fritzsedlazeck/SURVIVOR/wiki) + +> Jeffares, D., Jolly, C., Hoti, M. et al. Transient structural variations have strong effects on quantitative traits and reproductive isolation in fission yeast. Nat Commun 8, 14061 (2017). https://doi.org/10.1038/ncomms14061 + +- [SVanalyzer](https://svanalyzer.readthedocs.io/en/latest/index.html) + +- [svync](https://github.com/nvnieuwk/svync) + +- [tabix](https://www.htslib.org/doc/tabix.html) + +> Heng Li, Tabix: fast retrieval of sequence features from generic TAB-delimited files, Bioinformatics, Volume 27, Issue 5, March 2011, Pages 718–719, https://doi.org/10.1093/bioinformatics/btq671 + +- [truvari](https://github.com/ACEnglish/truvari) + +> English, A.C., Menon, V.K., Gibbs, R.A. et al. Truvari: refined structural variant comparison preserves allelic diversity. Genome Biol 23, 271 (2022). https://doi.org/10.1186/s13059-022-02840-6 + +- [UCSC](http://hgdownload.cse.ucsc.edu/admin/exe) + +> Hinrichs AS, Karolchik D, Baertsch R, Barber GP, Bejerano G, Clawson H, Diekhans M, Furey TS, Harte RA, Hsu F et al. The UCSC Genome Browser Database: update 2006. Nucleic Acids Res. 2006 Jan 1;34(Database issue):D590-8. + +- [witty.er](https://github.com/Illumina/witty.er) + +## R packages + +- [ggplot2](https://ggplot2.tidyverse.org/) + +> Wickham H (2016). ggplot2: Elegant Graphics for Data Analysis. Springer-Verlag New York. ISBN 978-3-319-24277-4, https://ggplot2.tidyverse.org. + +- [reshape2](https://cran.r-project.org/web/packages/reshape2/index.html) + +> Wickham H (2007). “Reshaping Data with the reshape Package.” Journal of Statistical Software, 21(12), 1–20. http://www.jstatsoft.org/v21/i12/. + +## Python packages + +- [pysam](https://pandas.pydata.org/) + ## Software packaging/containerisation tools - [Anaconda](https://anaconda.com) From 8bd708780d6dbf61795202b9be8d23c9f2a3c4f1 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 10:57:38 +0000 Subject: [PATCH 255/292] clean unused scripts --- bin/GangSTRConvertTruvariCompatible.py | 166 ----------- bin/annotate_repeat_regions.py | 71 ----- bin/convertInversion.py | 278 ------------------ bin/id_annotation.py | 52 ---- bin/simple_event_annotator.R | 59 ---- .../create_datavzrd_input/main.nf | 0 .../merge_reports/environment.yml | 0 .../local/{ => custom}/merge_reports/main.nf | 0 .../local/{ => custom}/plots/environment.yml | 0 modules/local/{ => custom}/plots/main.nf | 0 .../variant_extractor/environment.yml | 0 .../{ => custom}/variant_extractor/main.nf | 0 .../{ => custom}/vcf_to_csv/environment.yml | 0 modules/local/{ => custom}/vcf_to_csv/main.nf | 0 .../local/compare_benchmark_results.nf | 2 +- .../local/report_benchmark_statistics.nf | 6 +- subworkflows/local/sv_vcf_conversion.nf | 2 +- 17 files changed, 5 insertions(+), 631 deletions(-) delete mode 100755 bin/GangSTRConvertTruvariCompatible.py delete mode 100755 bin/annotate_repeat_regions.py delete mode 100755 bin/convertInversion.py delete mode 100755 bin/id_annotation.py delete mode 100755 bin/simple_event_annotator.R rename modules/local/{ => custom}/create_datavzrd_input/main.nf (100%) rename modules/local/{ => custom}/merge_reports/environment.yml (100%) rename modules/local/{ => custom}/merge_reports/main.nf (100%) rename modules/local/{ => custom}/plots/environment.yml (100%) rename modules/local/{ => custom}/plots/main.nf (100%) rename modules/local/{ => custom}/variant_extractor/environment.yml (100%) rename modules/local/{ => custom}/variant_extractor/main.nf (100%) rename modules/local/{ => custom}/vcf_to_csv/environment.yml (100%) rename modules/local/{ => custom}/vcf_to_csv/main.nf (100%) diff --git a/bin/GangSTRConvertTruvariCompatible.py b/bin/GangSTRConvertTruvariCompatible.py deleted file mode 100755 index 3ca6950..0000000 --- a/bin/GangSTRConvertTruvariCompatible.py +++ /dev/null @@ -1,166 +0,0 @@ -import argparse -import re -from Bio import SeqIO - -header = """##FILTER= -##INFO= -##INFO= -##INFO= -##EVENTTYPE= -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##INFO= -##FORMAT= -##FORMAT= -##ALT= -""" - - -def translateLine(line: str, ref: dict) -> str: - lineSplit = line.strip("\n").split("\t") - - contigsAndAlleles = lineSplit[:-3] - contigsAndAlleles[3] = ref[contigsAndAlleles[0]].seq[ - int(contigsAndAlleles[1]) - 1 - ] - contigsAndAlleles[4] = re.sub("[a-z]+", "", contigsAndAlleles[4]) - - infoField = lineSplit[-3] - infoDict = re.findall("([A-Z]+)=([\+\-A-Za-z\.\d_:]+);", infoField) - assert len(infoDict) != 0 - infoDict = dict(infoDict) - - contigsAndAlleles[2] = ( - "tr_" - + contigsAndAlleles[0] - + "_" - + contigsAndAlleles[1] - + "_" - + infoDict["END"] - ) - - formatKeys = lineSplit[-2].split(":") - if lineSplit[-1] == ".": - formatValues = ["."] * len(formatKeys) - else: - formatValues = lineSplit[-1].split(":") - formatDict = dict(zip(formatKeys, formatValues)) - - rul = len(infoDict["RU"]) - svlen = int(infoDict["END"]) - int(contigsAndAlleles[1]) - refruc = float(svlen) / rul - svlen = str(svlen) - repcn = formatDict["REPCN"].split(",") - - if formatDict["GT"] in ["./.", "."]: - cn = "." - ruc = "." - rucchange = "." - cnvtrlen = "." - cnformat = "." - else: - if formatDict["GT"] == "1/2": - svlen = [svlen] * 2 - ruc = [float(x) for x in repcn] - cn = [x / refruc for x in ruc] - rucchange = [x - refruc for x in ruc] - cnvtrlen = [x * rul for x in rucchange] - else: - svlen = [svlen] - ruc = ( - float(repcn[0]) - if formatDict["GT"] == "1/0" - else float(repcn[-1]) - ) - cn = [ruc / refruc] - rucchange = [ruc - refruc] - cnvtrlen = [rucchange[0] * rul] - ruc = [ruc] - cnformat = f"{sum(cn):.6f}" - svlen = ",".join(svlen) - cn = ",".join([f"{x:.2f}" for x in cn]) - ruc = ",".join([f"{x:.2f}" for x in ruc]) - rucchange = ",".join([f"{x:.6f}" for x in rucchange]) - cnvtrlen = ",".join([f"{x:.6f}" for x in cnvtrlen]) - - info = ["SVTYPE=CNV", "EVENTTYPE=STR"] - - info.append(f"SVLEN={svlen}") - info.append(f"END={infoDict['END']}") - info.append(f"REFRUC={refruc:.2f}") - info.append(f"RUS={infoDict['RU'].upper()}") - # RUL Gives issues with Wittyer (RulMismatch) not sure why - # info.append( - # f"RUL={len(infoDict['RU'])}" - # ) - info.append(f"CN={cn}") - info.append(f"RUC={ruc}") - info.append(f"RUCCHANGE={rucchange}") - info.append(f"CNVTRLEN={cnvtrlen}") - - info = ";".join(info) - - formatFields = ":".join(["GT", "CN"]) - - gt = formatDict["GT"] - gt = "0/1" if gt == "1/0" else gt - - format = ":".join([gt, cnformat]) - - """ - HOMREF calls have to be "masked" when using Witty.er, otherwise a HOMREF call that is not - present in the truth will be counted as a query FP, decreasing precision for no reason. - It's sufficient to change the FILTER from PASS or . to something else (like HOMREF) - """ - contigsAndAlleles[6] = "HOMREF" if gt in ["0/0", "0"] else "PASS" - - contigsAndAlleles = "\t".join(contigsAndAlleles) - return "\t".join([contigsAndAlleles, info, formatFields, format]) + "\n" - - -def convertVcf(inputFile: str, outputFile: str, reference: str) -> None: - inputHandle = open(inputFile, "r") - outputHandle = open(outputFile, "w") - - referenceDict = SeqIO.to_dict(SeqIO.parse(reference, "fasta")) - - for line in inputHandle: - if re.search("^#[^#]", line): - outputHandle.write(header) - outputHandle.write(line) - continue - - if re.search("^##(INFO)|(FORMAT)|(ALT)", line): - continue - - if re.search("^##", line): - outputHandle.write(line) - continue - - outputHandle.write(translateLine(line, referenceDict)) - - inputHandle.close() - outputHandle.close() - - -if __name__ == "__main__": - parser = argparse.ArgumentParser( - description="Convert the repeat genotyping module VCF output in a VCFv4.4 compliant format (similar to the VNTR module output)" - ) - parser.add_argument( - "-i", "--input", help="input VCF", type=str, required=True - ) - parser.add_argument( - "-r", "--reference", help="Genome FASTA", type=str, required=False - ) - parser.add_argument( - "-o", "--output", help="output VCF", type=str, required=True - ) - args = parser.parse_args() - - convertVcf(args.input, args.output, args.reference) diff --git a/bin/annotate_repeat_regions.py b/bin/annotate_repeat_regions.py deleted file mode 100755 index 1002660..0000000 --- a/bin/annotate_repeat_regions.py +++ /dev/null @@ -1,71 +0,0 @@ -import pysam -import pandas as pd -import numpy as np -import matplotlib.pyplot as plt -import argparse -import os - -parser = argparse.ArgumentParser() -parser.add_argument('--vcf', required=True, help='Input VCF') -parser.add_argument('--repeat', required=True, help='Simple Repeat regions') -parser.add_argument('--repmask', required=True, help='Repeat Mask regions') -parser.add_argument('--segdup', required=True, help='Segmental Duplication regions') - -args = parser.parse_args - -def print_AF(in_vcf,samplerep,repeatmask,segdup): - in_file = pysam.VariantFile(in_vcf) - out_name = os.path.basename(in_vcf) - if out_name.endswith('.gz'): - out_name = out_name[:-3] - if out_name.endswith('.vcf'): - out_name = out_name[:-4] - - with open(out_name + ".txt","w") as out_file: - - print('SV_Type', 'SV', 'Length' , 'AF', 'Simple_Repeat','Repeat_Name','Repeat_Class','Segmental_Dup', file=out_file) - for v in in_vcf: - temp1=samplerep[(samplerep['chrom']== v.chrom) &( (( v.start > samplerep['chromStart']-1 ) & ( v.start < samplerep['chromEnd'] +1 ) )| (( v.stop > samplerep['chromStart'] -1) & ( v.stop < samplerep['chromEnd'] +1 ))) ] - - if len(temp1) > 0: - simple_repeat=temp1.iloc[0].period - else: - simple_repeat='None' - - temp2=repeatmask[(repeatmask['genoName']== v.chrom) &( (( v.start > repeatmask['genoStart']-1 ) & ( v.start < repeatmask['genoEnd'] +1 ) )| (( v.stop > repeatmask['genoStart'] -1) & ( v.stop < repeatmask['genoEnd'] +1 ))) ] - - if len(temp2) > 0: - repname=temp2.iloc[0].repName - repclass=temp2.iloc[0].repClass - else: - repname='None' - repclass='None' - - temp3=segdup[(segdup['chrom']== v.chrom) &( (( v.start > segdup['chromStart']-1 ) & ( v.start < segdup['chromEnd'] +1 ) )| (( v.stop > segdup['chromStart'] -1) & ( v.stop < segdup['chromEnd'] +1 ))) ] - if len(temp3) > 0: - segdups=temp3.iloc[0].fracMatch - else: - segdups='None' - - - if len(v.ref) - len(v.alts[0]) > 49: - if len(v.ref) - len(v.alts[0]) < 1001: - print('DEL', v.ref, len(v.alts[0])- len(v.ref) , v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) - else: - print('DEL', '...', len(v.alts[0])- len(v.ref) , v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) - - elif len(v.alts[0]) - len(v.ref) > 49: - if len(v.ref) - len(v.alts[0]) < 1001: - print('INS', v.alts[0],len(v.alts[0]) - len(v.ref), v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) - else: - print('INS', '...',len(v.alts[0]) - len(v.ref), v.info['AF'][0],simple_repeat,repname, repclass,segdups, file=out_file) - - else: - continue - - -repeat_file = pd.read_csv(args.repeat, header=0, sep='\t') -repmask_file = pd.read_csv(args.repmask, header=0, sep='\t') -segdup_file = pd.read_csv(args.segdups, header=0, sep='\t') - -print_AF(args.vcf, repeat_file,repmask_file,segdup_file ) diff --git a/bin/convertInversion.py b/bin/convertInversion.py deleted file mode 100755 index 8f70cdf..0000000 --- a/bin/convertInversion.py +++ /dev/null @@ -1,278 +0,0 @@ -#!/usr/bin/env python2 -# - - -import sys -import gzip -from io import BufferedReader -from subprocess import check_output -from os import path -from os.path import exists - - -class VcfRecord: - - def __init__(self, inline): - tokens = inline.strip().split('\t') - - self.chr = tokens[0] - self.pos = int(tokens[1]) - self.vid = tokens[2] - self.ref = tokens[3] - self.alt = tokens[4] - self.qual = tokens[5] - self.filter = tokens[6] - self.info = tokens[7].split(';') - self.others = "\t".join(tokens[8:]) - - # Create a dictionary for INFO - self.infoDict ={} - for infoItem in self.info: - items = infoItem.split('=') - if len(items) == 1: - self.infoDict[items[0]] = True - elif len(items) > 1: - self.infoDict[items[0]] = items[1] - - self.isINV3 = False - self.isINV5 = False - self.mateChr = "" - self.matePos = -1 - - - def checkInversion(self): - - def getMateInfo(splitChar): - items = self.alt.split(splitChar) - mateInfo = items[1].split(':') - # Assuming that the last item, contains position information - matePos = mateInfo[-1] - # Other items belong to chromosome - self.mateChr = ":".join(mateInfo[:-1]) - self.matePos = int(matePos) - - if self.alt.startswith('['): - getMateInfo('[') - if self.mateChr == self.chr: - self.isINV5 = True - elif self.alt.endswith(']'): - getMateInfo(']') - if self.mateChr == self.chr: - self.isINV3 = True - - - def makeLine(self): - infoStr = ";".join(self.info) - - self.line = "\t".join((self.chr, - str(self.pos), - self.vid, - self.ref, - self.alt, - self.qual, - self.filter, - infoStr, - self.others - ))+"\n" - - -def scanVcf(vcfFile): - - invMateDict = {} - - if vcfFile.endswith('gz'): - gzfp = gzip.open(vcfFile, 'rb') - fpVcf = BufferedReader(gzfp) - else: - fpVcf = open(vcfFile, 'rb') - - for line in fpVcf: - if line[0] == '#': - continue - - vcfRec = VcfRecord(line) - vcfRec.checkInversion() - if vcfRec.isINV3 or vcfRec.isINV5: - if vcfRec.vid in invMateDict: - # update mate INFO - invMateDict[vcfRec.vid] = vcfRec.infoDict - else: - mateId = vcfRec.infoDict["MATEID"] - invMateDict[mateId] = "" - - return invMateDict - - -def getReference(samtools, refFasta, chrom, start, end): - region = "%s:%d-%d" % (chrom, start, end) - samtoolsOut = check_output([samtools, "faidx", refFasta, region]) - refSeq = "" - for seq in samtoolsOut.split('\n'): - if not seq.startswith(">"): - refSeq += seq - - return refSeq.upper() - - -def writeLines(lines): - for line in lines: - sys.stdout.write(line) - - -def convertInversions(samtools, refFasta, vcfFile, invMateDict): - isHeaderInfoAdded = False - isHeaderAltAdded = False - lineBuffer = [] - bufferedChr = "" - bufferedPos = -1 - - if vcfFile.endswith('gz'): - gzfp = gzip.open(vcfFile, 'rb') - fpVcf = BufferedReader(gzfp) - else: - fpVcf = open(vcfFile, 'rb') - - for line in fpVcf: - if line.startswith('#'): - if (not isHeaderInfoAdded) and line.startswith("##FORMAT="): - sys.stdout.write("##INFO=\n") - sys.stdout.write("##INFO=\n") - isHeaderInfoAdded = True - - if (not isHeaderAltAdded) and line.startswith("##ALT="): - sys.stdout.write("##ALT=\n") - isHeaderAltAdded = True - - sys.stdout.write(line) - continue - - vcfRec = VcfRecord(line) - - # skip mate record - if vcfRec.vid in invMateDict: - continue - - vcfRec.checkInversion() - if vcfRec.isINV3 or vcfRec.isINV5: - if vcfRec.isINV5: - # adjust POS for INV5 - vcfRec.pos -= 1 - vcfRec.matePos -= 1 - vcfRec.ref = getReference(samtools, refFasta, - vcfRec.chr, vcfRec.pos, vcfRec.pos) - - # update manta ID - vidSuffix = vcfRec.vid.split("MantaBND")[1] - idx = vidSuffix.rfind(':') - vcfRec.vid = "MantaINV%s" % vidSuffix[:idx] - - # symbolic ALT - vcfRec.alt = "" - - # add END - infoEndStr = "END=%d" % vcfRec.matePos - - newInfo = [infoEndStr] - for infoItem in vcfRec.info: - if infoItem.startswith("SVTYPE"): - # change SVTYPE - newInfo.append("SVTYPE=INV") - # add SVLEN - infoSvLenStr = "SVLEN=%d" % (vcfRec.matePos-vcfRec.pos) - newInfo.append(infoSvLenStr) - - elif infoItem.startswith("CIPOS"): - newInfo.append(infoItem) - - # set CIEND - isImprecise = "IMPRECISE" in vcfRec.infoDict - # for imprecise calls, set CIEND to the mate breakpoint's CIPOS - if isImprecise: - mateId = vcfRec.infoDict["MATEID"] - mateInfoDict = invMateDict[mateId] - infoCiEndStr = "CIEND=%s" % (mateInfoDict["CIPOS"]) - newInfo.append(infoCiEndStr) - # for precise calls, set CIEND w.r.t HOMLEN - else: - if "HOMLEN" in vcfRec.infoDict: - infoCiEndStr = "CIEND=-%s,0" % vcfRec.infoDict["HOMLEN"] - newInfo.append(infoCiEndStr) - - elif infoItem.startswith("HOMSEQ"): - # update HOMSEQ for INV5 - if vcfRec.isINV5: - cipos = vcfRec.infoDict["CIPOS"].split(',') - homSeqStart = vcfRec.pos + int(cipos[0]) + 1 - homSeqEnd = vcfRec.pos + int(cipos[1]) - refSeq = getReference(samtools, refFasta, vcfRec.chr, - homSeqStart, homSeqEnd) - infoHomSeqStr = "HOMSEQ=%s" % refSeq - newInfo.append(infoHomSeqStr) - else: - newInfo.append(infoItem) - - # skip BND-specific tags - elif (infoItem.startswith("MATEID") or - infoItem.startswith("BND_DEPTH") or - infoItem.startswith("MATE_BND_DEPTH")): - continue - - # update event ID - elif infoItem.startswith("EVENT"): - eidSuffix = vcfRec.infoDict["EVENT"].split("MantaBND")[1] - idx = vidSuffix.rfind(':') - infoEventStr = "EVENT=MantaINV%s" % eidSuffix[:idx] - newInfo.append(infoEventStr) - - # apply all other tags - else: - newInfo.append(infoItem) - - # add INV3/INV5 tag - if vcfRec.isINV3: - newInfo.append("INV3") - elif vcfRec.isINV5: - newInfo.append("INV5") - - vcfRec.info = newInfo - - vcfRec.makeLine() - - # make sure the vcf is sorted in genomic order - if (not vcfRec.chr == bufferedChr) or (vcfRec.pos > bufferedPos): - if lineBuffer: - writeLines(lineBuffer) - - lineBuffer = [vcfRec.line] - bufferedChr = vcfRec.chr - bufferedPos = vcfRec.pos - elif vcfRec.pos < bufferedPos: - lineBuffer.insert(0, vcfRec.line) - else: - lineBuffer.append(vcfRec.line) - - if lineBuffer: - writeLines(lineBuffer) - - - -if __name__=='__main__': - - usage = "convertInversion.py \n" - if len(sys.argv) <= 3: - sys.stderr.write(usage) - sys.exit(1) - - samtools = sys.argv[1] - refFasta = sys.argv[2] - vcfFile = sys.argv[3] - - for inputFile in [samtools, refFasta, vcfFile]: - if not(exists(inputFile)): - errMsg = ('File %s does not exist.' - % inputFile) - sys.stderr.write(errMsg + '\nProgram exits.') - sys.exit(1) - - invMateDict = scanVcf(vcfFile) - convertInversions(samtools, refFasta, vcfFile, invMateDict) diff --git a/bin/id_annotation.py b/bin/id_annotation.py deleted file mode 100755 index a41e653..0000000 --- a/bin/id_annotation.py +++ /dev/null @@ -1,52 +0,0 @@ -import argparse -import pysam -import logging -import os -import subprocess as sp - -logging.basicConfig( - format='%(levelname)-7s | %(asctime)s | %(message)s', - datefmt='%H:%M:%S') -logger = logging.getLogger(__name__) -logger.setLevel(logging.INFO) - - -parser = argparse.ArgumentParser() -parser.add_argument('--vcf', required=True, help='Input VCF') -args = parser.parse_args() - - - -in_vcf = pysam.VariantFile(args.vcf) -out_name = os.path.basename(args.vcf) -if out_name.endswith('.gz'): - out_name = out_name[:-3] -if out_name.endswith('.vcf'): - out_name = out_name[:-4] - -anno_header = in_vcf.header - -anno_vcf = pysam.VariantFile('{}.annotated.vcf.gz'.format(out_name), 'w', header=anno_header) - -logger.info('Adding CHROM_POS_TYPE to ID') -for v in in_vcf: - svlen=(len(v.alts[0])-len(v.ref)) - if (svlen < 0): - string=str(v.chrom) +'_'+ str(v.pos) + '_DEL' - v.id =string - elif (svlen > 0): - string=str(v.chrom) +'_'+ str(v.pos) + '_INS' - v.id =string - else: - string=str(v.chrom) +'_'+ str(v.pos) + '_SNP' - v.id =string - - -in_vcf.close() -anno_vcf.close() -logger.info('Finished') - -sp.run(['tabix', anno_vcf.filename]) -logger.info('VCF indexed') - -logger.info('DONE') diff --git a/bin/simple_event_annotator.R b/bin/simple_event_annotator.R deleted file mode 100755 index 1325014..0000000 --- a/bin/simple_event_annotator.R +++ /dev/null @@ -1,59 +0,0 @@ -#!/usr/bin/env Rscript -# -# Copyright GRIDSS -## original version : https://github.com/PapenfussLab/gridss/blob/7b1fedfed32af9e03ed5c6863d368a821a4c699f/example/simple-event-annotation.R - -#source("https://bioconductor.org/biocLite.R") -#biocLite("VariantAnnotation") -#install_github("PapenfussLab/StructuralVariantAnnotation") -#install.packages("stringr") -library(VariantAnnotation) -library(StructuralVariantAnnotation) -library(stringr) -library(getopt) -#' Simple SV type classifier -## USAGE -## simple_event_annotator.R infile outfile genome - - -simpleEventType <- function(gr) { - return(ifelse(seqnames(gr) != seqnames(partner(gr)), "ITX", # inter-chromosomosal - ifelse(gr$insLen >= abs(gr$svLen) * 0.7, "INS", # TODO: improve classification of complex events - ifelse(strand(gr) == strand(partner(gr)), "INV", - ifelse(xor(start(gr) < start(partner(gr)), strand(gr) == "-"), "DEL", - "DUP"))))) -} -cmdArgs = commandArgs(TRUE) -print(cmdArgs) -if (length(cmdArgs) < 3) print(paste("Incorrect number of arguments (3 expected): ",length(cmdArgs))) -infile = cmdArgs[1] -outfile = cmdArgs[2] -genome = cmdArgs[3] - - -# using the example in the GRIDSS /example directory -vcf <- readVcf(infile, genome) -gr <- breakpointRanges(vcf) -svtype <- simpleEventType(gr) -info(vcf)$SIMPLE_TYPE <- NA_character_ -info(vcf[gr$vcfId])$SIMPLE_TYPE <- svtype -info(vcf[gr$vcfId])$SVLEN <- gr$svLen -writeVcf(vcf, outfile) - -## TODO: perform event filtering here -## By default, GRIDSS is very sensitive but this comes at the cost of a high false discovery rate -#gr <- gr[gr$FILTER == "PASS" & partner(gr)$FILTER == "PASS"] # Remove low confidence calls - -#simplegr <- gr[simpleEventType(gr) %in% c("INS", "INV", "DEL", "DUP")] -#simplebed <- data.frame( -# chrom=seqnames(simplegr), -# # call the centre of the homology/inexact interval -# start=as.integer((start(simplegr) + end(simplegr)) / 2), -# end=as.integer((start(partner(simplegr)) + end(partner(simplegr))) / 2), -# name=simpleEventType(simplegr), -# score=simplegr$QUAL, -# strand="." -# ) -## Just the lower of the two breakends so we don't output everything twice -#simplebed <- simplebed[simplebed$start < simplebed$end,] -#write.table(simplebed, "chr12.1527326.DEL1024.simple.bed", quote=FALSE, sep='\t', row.names=FALSE, col.names=FALSE) diff --git a/modules/local/create_datavzrd_input/main.nf b/modules/local/custom/create_datavzrd_input/main.nf similarity index 100% rename from modules/local/create_datavzrd_input/main.nf rename to modules/local/custom/create_datavzrd_input/main.nf diff --git a/modules/local/merge_reports/environment.yml b/modules/local/custom/merge_reports/environment.yml similarity index 100% rename from modules/local/merge_reports/environment.yml rename to modules/local/custom/merge_reports/environment.yml diff --git a/modules/local/merge_reports/main.nf b/modules/local/custom/merge_reports/main.nf similarity index 100% rename from modules/local/merge_reports/main.nf rename to modules/local/custom/merge_reports/main.nf diff --git a/modules/local/plots/environment.yml b/modules/local/custom/plots/environment.yml similarity index 100% rename from modules/local/plots/environment.yml rename to modules/local/custom/plots/environment.yml diff --git a/modules/local/plots/main.nf b/modules/local/custom/plots/main.nf similarity index 100% rename from modules/local/plots/main.nf rename to modules/local/custom/plots/main.nf diff --git a/modules/local/variant_extractor/environment.yml b/modules/local/custom/variant_extractor/environment.yml similarity index 100% rename from modules/local/variant_extractor/environment.yml rename to modules/local/custom/variant_extractor/environment.yml diff --git a/modules/local/variant_extractor/main.nf b/modules/local/custom/variant_extractor/main.nf similarity index 100% rename from modules/local/variant_extractor/main.nf rename to modules/local/custom/variant_extractor/main.nf diff --git a/modules/local/vcf_to_csv/environment.yml b/modules/local/custom/vcf_to_csv/environment.yml similarity index 100% rename from modules/local/vcf_to_csv/environment.yml rename to modules/local/custom/vcf_to_csv/environment.yml diff --git a/modules/local/vcf_to_csv/main.nf b/modules/local/custom/vcf_to_csv/main.nf similarity index 100% rename from modules/local/vcf_to_csv/main.nf rename to modules/local/custom/vcf_to_csv/main.nf diff --git a/subworkflows/local/compare_benchmark_results.nf b/subworkflows/local/compare_benchmark_results.nf index 9aa790e..60647ba 100644 --- a/subworkflows/local/compare_benchmark_results.nf +++ b/subworkflows/local/compare_benchmark_results.nf @@ -5,7 +5,7 @@ include { SURVIVOR_MERGE } from '../../modules/nf-core/survivor/merge' include { BCFTOOLS_MERGE } from '../../modules/nf-core/bcftools/merge' -include { VCF_TO_CSV } from '../../modules/local/vcf_to_csv' +include { VCF_TO_CSV } from '../../modules/local/custom/vcf_to_csv' include { REFORMAT_HEADER } from '../../modules/local/custom/reformat_header' include { TABIX_BGZIP as TABIX_BGZIP_UNZIP } from '../../modules/nf-core/tabix/bgzip' diff --git a/subworkflows/local/report_benchmark_statistics.nf b/subworkflows/local/report_benchmark_statistics.nf index cb3cad5..c55fcf6 100644 --- a/subworkflows/local/report_benchmark_statistics.nf +++ b/subworkflows/local/report_benchmark_statistics.nf @@ -2,9 +2,9 @@ // REPORT_BENCHMARK_STATISTICS: SUMMARIZE BENCHMARK REPORTS // -include { MERGE_REPORTS } from '../../modules/local/merge_reports' -include { PLOTS } from '../../modules/local/plots' -include { CREATE_DATAVZRD_INPUT } from '../../modules/local/create_datavzrd_input' +include { MERGE_REPORTS } from '../../modules/local/custom/merge_reports' +include { PLOTS } from '../../modules/local/custom/plots' +include { CREATE_DATAVZRD_INPUT } from '../../modules/local/custom/create_datavzrd_input' include { DATAVZRD } from '../../modules/nf-core/datavzrd' workflow REPORT_BENCHMARK_STATISTICS { diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion.nf index 92e08cd..a236f36 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion.nf @@ -6,7 +6,7 @@ import groovy.io.FileType include { SVYNC } from '../../modules/nf-core/svync' include { BGZIP_TABIX } from '../../modules/local/bgzip/tabix' -include { VARIANT_EXTRACTOR } from '../../modules/local/variant_extractor' +include { VARIANT_EXTRACTOR } from '../../modules/local/custom/variant_extractor' include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' workflow SV_VCF_CONVERSIONS { From 924c51753291c95e3612a7888ddbdec9ee9e407b Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 12:09:51 +0000 Subject: [PATCH 256/292] correcting lint warnings --- nextflow_schema.json | 2 +- .../main.nf} | 6 +++--- .../main.nf} | 10 +++++----- .../main.nf} | 14 ++++++------- .../main.nf} | 20 +++++++++---------- .../main.nf} | 12 +++++------ .../main.nf} | 8 ++++---- .../main.nf} | 4 ++-- .../main.nf} | 16 +++++++-------- .../main.nf} | 4 ++-- .../main.nf} | 8 ++++---- .../main.nf} | 6 +++--- .../main.nf} | 18 ++++++++--------- .../main.nf} | 2 +- .../main.nf} | 8 ++++---- .../main.nf} | 4 ++-- .../main.nf} | 8 ++++---- 17 files changed, 75 insertions(+), 75 deletions(-) rename subworkflows/local/{cnv_germline_benchmark.nf => cnv_germline_benchmark/main.nf} (85%) rename subworkflows/local/{compare_benchmark_results.nf => compare_benchmark_results/main.nf} (81%) rename subworkflows/local/{liftover_vcfs.nf => liftover_vcfs/main.nf} (76%) rename subworkflows/local/{prepare_vcfs_test.nf => prepare_vcfs_test/main.nf} (86%) rename subworkflows/local/{prepare_vcfs_truth.nf => prepare_vcfs_truth/main.nf} (86%) rename subworkflows/local/{report_benchmark_statistics.nf => report_benchmark_statistics/main.nf} (81%) rename subworkflows/local/{report_vcf_statistics.nf => report_vcf_statistics/main.nf} (88%) rename subworkflows/local/{small_germline_benchmark.nf => small_germline_benchmark/main.nf} (92%) rename subworkflows/local/{small_somatic_benchmark.nf => small_somatic_benchmark/main.nf} (91%) rename subworkflows/local/{split_small_variants_test.nf => split_small_variants_test/main.nf} (84%) rename subworkflows/local/{subsample_vcf_test.nf => subsample_vcf_test/main.nf} (88%) rename subworkflows/local/{sv_germline_benchmark.nf => sv_germline_benchmark/main.nf} (92%) rename subworkflows/local/{sv_somatic_benchmark.nf => sv_somatic_benchmark/main.nf} (92%) rename subworkflows/local/{sv_vcf_conversion.nf => sv_vcf_conversion/main.nf} (88%) rename subworkflows/local/{vcf_variant_deduplication.nf => vcf_variant_deduplication/main.nf} (81%) rename subworkflows/local/{vcf_variant_filtering.nf => vcf_variant_filtering/main.nf} (84%) diff --git a/nextflow_schema.json b/nextflow_schema.json index 28738e8..0df663d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -2,7 +2,7 @@ "$schema": "https://json-schema.org/draft/2020-12/schema", "$id": "https://raw.githubusercontent.com/nf-core/variantbenchmarking/master/nextflow_schema.json", "title": "nf-core/variantbenchmarking pipeline parameters", - "description": "Master benchmarking pipeline for variant callers ", + "description": "Variant Benchmarking pipeline for germline and somatic variant callers", "type": "object", "$defs": { "input_output_options": { diff --git a/subworkflows/local/cnv_germline_benchmark.nf b/subworkflows/local/cnv_germline_benchmark/main.nf similarity index 85% rename from subworkflows/local/cnv_germline_benchmark.nf rename to subworkflows/local/cnv_germline_benchmark/main.nf index c1e631d..1b2e102 100644 --- a/subworkflows/local/cnv_germline_benchmark.nf +++ b/subworkflows/local/cnv_germline_benchmark/main.nf @@ -2,9 +2,9 @@ // CNV_GERMLINE_BENCHMARK: SUBWORKFLOW FOR CNV GERMLINE VARIANTS // -include { WITTYER } from '../../modules/nf-core/wittyer' -include { TABIX_BGZIP as TABIX_BGZIP_QUERY } from '../../modules/nf-core/tabix/bgzip' -include { TABIX_BGZIP as TABIX_BGZIP_TRUTH } from '../../modules/nf-core/tabix/bgzip' +include { WITTYER } from '../../../modules/nf-core/wittyer' +include { TABIX_BGZIP as TABIX_BGZIP_QUERY } from '../../../modules/nf-core/tabix/bgzip' +include { TABIX_BGZIP as TABIX_BGZIP_TRUTH } from '../../../modules/nf-core/tabix/bgzip' workflow CNV_GERMLINE_BENCHMARK { take: diff --git a/subworkflows/local/compare_benchmark_results.nf b/subworkflows/local/compare_benchmark_results/main.nf similarity index 81% rename from subworkflows/local/compare_benchmark_results.nf rename to subworkflows/local/compare_benchmark_results/main.nf index 60647ba..39aa1c3 100644 --- a/subworkflows/local/compare_benchmark_results.nf +++ b/subworkflows/local/compare_benchmark_results/main.nf @@ -3,11 +3,11 @@ // COMPARE_BENCHMARK_RESULTS: SUBWORKFLOW to merge TP/FP/FN results from different tools. // -include { SURVIVOR_MERGE } from '../../modules/nf-core/survivor/merge' -include { BCFTOOLS_MERGE } from '../../modules/nf-core/bcftools/merge' -include { VCF_TO_CSV } from '../../modules/local/custom/vcf_to_csv' -include { REFORMAT_HEADER } from '../../modules/local/custom/reformat_header' -include { TABIX_BGZIP as TABIX_BGZIP_UNZIP } from '../../modules/nf-core/tabix/bgzip' +include { SURVIVOR_MERGE } from '../../../modules/nf-core/survivor/merge' +include { BCFTOOLS_MERGE } from '../../../modules/nf-core/bcftools/merge' +include { VCF_TO_CSV } from '../../../modules/local/custom/vcf_to_csv' +include { REFORMAT_HEADER } from '../../../modules/local/custom/reformat_header' +include { TABIX_BGZIP as TABIX_BGZIP_UNZIP } from '../../../modules/nf-core/tabix/bgzip' workflow COMPARE_BENCHMARK_RESULTS { take: diff --git a/subworkflows/local/liftover_vcfs.nf b/subworkflows/local/liftover_vcfs/main.nf similarity index 76% rename from subworkflows/local/liftover_vcfs.nf rename to subworkflows/local/liftover_vcfs/main.nf index 7bf4331..c357af7 100644 --- a/subworkflows/local/liftover_vcfs.nf +++ b/subworkflows/local/liftover_vcfs/main.nf @@ -2,13 +2,13 @@ // LIFTOVER_VCFS: SUBWORKFLOW TO LIFTOVER VCFS HG37 TO HG38 OR HG38 TO HG37 // -include { PICARD_CREATESEQUENCEDICTIONARY } from '../../modules/nf-core/picard/createsequencedictionary' -include { PICARD_LIFTOVERVCF } from '../../modules/nf-core/picard/liftovervcf' -include { REFORMAT_HEADER } from '../../modules/local/custom/reformat_header' -include { BCFTOOLS_RENAME_CHR } from '../../modules/local/bcftools/rename_chr' -include { UCSC_LIFTOVER } from '../../modules/nf-core/ucsc/liftover' -include { SORT_BED } from '../../modules/local/custom/sort_bed' -include { BEDTOOLS_MERGE } from '../../modules/nf-core/bedtools/merge' +include { PICARD_CREATESEQUENCEDICTIONARY } from '../../../modules/nf-core/picard/createsequencedictionary' +include { PICARD_LIFTOVERVCF } from '../../../modules/nf-core/picard/liftovervcf' +include { REFORMAT_HEADER } from '../../../modules/local/custom/reformat_header' +include { BCFTOOLS_RENAME_CHR } from '../../../modules/local/bcftools/rename_chr' +include { UCSC_LIFTOVER } from '../../../modules/nf-core/ucsc/liftover' +include { SORT_BED } from '../../../modules/local/custom/sort_bed' +include { BEDTOOLS_MERGE } from '../../../modules/nf-core/bedtools/merge' workflow LIFTOVER_VCFS { diff --git a/subworkflows/local/prepare_vcfs_test.nf b/subworkflows/local/prepare_vcfs_test/main.nf similarity index 86% rename from subworkflows/local/prepare_vcfs_test.nf rename to subworkflows/local/prepare_vcfs_test/main.nf index cdd9b9c..b748a40 100644 --- a/subworkflows/local/prepare_vcfs_test.nf +++ b/subworkflows/local/prepare_vcfs_test/main.nf @@ -2,16 +2,16 @@ // PREPARE_VCFS: SUBWORKFLOW TO PREPARE INPUT VCFS // -include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' -include { VCF_VARIANT_FILTERING } from '../local/vcf_variant_filtering' -include { SPLIT_SMALL_VARIANTS_TEST } from '../local/split_small_variants_test' -include { LIFTOVER_VCFS } from '../local/liftover_vcfs' -include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' -include { FIX_VCF_PREFIX } from '../../modules/local/custom/fix_vcf_prefix' -include { PUBLISH_PROCESSED_VCF } from '../../modules/local/custom/publish_processed_vcf' -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_CONTIGS } from '../../modules/nf-core/bcftools/view' -include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../modules/nf-core/bcftools/norm' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_QUERY} from '../../modules/nf-core/bcftools/reheader' +include { VCF_VARIANT_DEDUPLICATION } from '../../local/vcf_variant_deduplication' +include { VCF_VARIANT_FILTERING } from '../../local/vcf_variant_filtering' +include { SPLIT_SMALL_VARIANTS_TEST } from '../../local/split_small_variants_test' +include { LIFTOVER_VCFS } from '../../local/liftover_vcfs' +include { BCFTOOLS_NORM } from '../../../modules/nf-core/bcftools/norm' +include { FIX_VCF_PREFIX } from '../../../modules/local/custom/fix_vcf_prefix' +include { PUBLISH_PROCESSED_VCF } from '../../../modules/local/custom/publish_processed_vcf' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_CONTIGS } from '../../../modules/nf-core/bcftools/view' +include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../../modules/nf-core/bcftools/norm' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_QUERY} from '../../../modules/nf-core/bcftools/reheader' workflow PREPARE_VCFS_TEST { diff --git a/subworkflows/local/prepare_vcfs_truth.nf b/subworkflows/local/prepare_vcfs_truth/main.nf similarity index 86% rename from subworkflows/local/prepare_vcfs_truth.nf rename to subworkflows/local/prepare_vcfs_truth/main.nf index bbf3834..60cd3c5 100644 --- a/subworkflows/local/prepare_vcfs_truth.nf +++ b/subworkflows/local/prepare_vcfs_truth/main.nf @@ -3,12 +3,12 @@ // -include { VCF_VARIANT_DEDUPLICATION } from '../local/vcf_variant_deduplication' -include { LIFTOVER_VCFS } from '../local/liftover_vcfs' -include { BCFTOOLS_NORM } from '../../modules/nf-core/bcftools/norm' -include { PUBLISH_PROCESSED_VCF } from '../../modules/local/custom/publish_processed_vcf' -include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../modules/nf-core/bcftools/norm' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TRUTH} from '../../modules/nf-core/bcftools/reheader' +include { VCF_VARIANT_DEDUPLICATION } from '../../local/vcf_variant_deduplication' +include { LIFTOVER_VCFS } from '../../local/liftover_vcfs' +include { BCFTOOLS_NORM } from '../../../modules/nf-core/bcftools/norm' +include { PUBLISH_PROCESSED_VCF } from '../../../modules/local/custom/publish_processed_vcf' +include { BCFTOOLS_NORM as BCFTOOLS_SPLIT_MULTI } from '../../../modules/nf-core/bcftools/norm' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_TRUTH} from '../../../modules/nf-core/bcftools/reheader' workflow PREPARE_VCFS_TRUTH { diff --git a/subworkflows/local/report_benchmark_statistics.nf b/subworkflows/local/report_benchmark_statistics/main.nf similarity index 81% rename from subworkflows/local/report_benchmark_statistics.nf rename to subworkflows/local/report_benchmark_statistics/main.nf index c55fcf6..d2e5563 100644 --- a/subworkflows/local/report_benchmark_statistics.nf +++ b/subworkflows/local/report_benchmark_statistics/main.nf @@ -2,10 +2,10 @@ // REPORT_BENCHMARK_STATISTICS: SUMMARIZE BENCHMARK REPORTS // -include { MERGE_REPORTS } from '../../modules/local/custom/merge_reports' -include { PLOTS } from '../../modules/local/custom/plots' -include { CREATE_DATAVZRD_INPUT } from '../../modules/local/custom/create_datavzrd_input' -include { DATAVZRD } from '../../modules/nf-core/datavzrd' +include { MERGE_REPORTS } from '../../../modules/local/custom/merge_reports' +include { PLOTS } from '../../../modules/local/custom/plots' +include { CREATE_DATAVZRD_INPUT } from '../../../modules/local/custom/create_datavzrd_input' +include { DATAVZRD } from '../../../modules/nf-core/datavzrd' workflow REPORT_BENCHMARK_STATISTICS { take: diff --git a/subworkflows/local/report_vcf_statistics.nf b/subworkflows/local/report_vcf_statistics/main.nf similarity index 88% rename from subworkflows/local/report_vcf_statistics.nf rename to subworkflows/local/report_vcf_statistics/main.nf index dc89afc..e104d9f 100644 --- a/subworkflows/local/report_vcf_statistics.nf +++ b/subworkflows/local/report_vcf_statistics/main.nf @@ -2,8 +2,8 @@ // REPORT_VCF_STATISTICS: SUBWORKFLOW TO REPORT VCF STATS // -include { SURVIVOR_STATS } from '../../modules/nf-core/survivor/stats' -include { BCFTOOLS_STATS } from '../../modules/nf-core/bcftools/stats' +include { SURVIVOR_STATS } from '../../../modules/nf-core/survivor/stats' +include { BCFTOOLS_STATS } from '../../../modules/nf-core/bcftools/stats' workflow REPORT_VCF_STATISTICS { take: diff --git a/subworkflows/local/small_germline_benchmark.nf b/subworkflows/local/small_germline_benchmark/main.nf similarity index 92% rename from subworkflows/local/small_germline_benchmark.nf rename to subworkflows/local/small_germline_benchmark/main.nf index c8c6ab6..0f11c1e 100644 --- a/subworkflows/local/small_germline_benchmark.nf +++ b/subworkflows/local/small_germline_benchmark/main.nf @@ -2,14 +2,14 @@ // SMALL_GERMLINE_BENCHMARK: SUBWORKFLOW FOR SMALL GERMLINE VARIANTS // -include { RTGTOOLS_FORMAT } from '../../modules/nf-core/rtgtools/format/main' -include { RTGTOOLS_VCFEVAL } from '../../modules/nf-core/rtgtools/vcfeval/main' -include { HAPPY_HAPPY } from '../../modules/nf-core/happy/happy/main' -include { HAPPY_PREPY } from '../../modules/nf-core/happy/prepy/main' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_1 } from '../../modules/nf-core/bcftools/reheader' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_2 } from '../../modules/nf-core/bcftools/reheader' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_3 } from '../../modules/nf-core/bcftools/reheader' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_4 } from '../../modules/nf-core/bcftools/reheader' +include { RTGTOOLS_FORMAT } from '../../../modules/nf-core/rtgtools/format/main' +include { RTGTOOLS_VCFEVAL } from '../../../modules/nf-core/rtgtools/vcfeval/main' +include { HAPPY_HAPPY } from '../../../modules/nf-core/happy/happy/main' +include { HAPPY_PREPY } from '../../../modules/nf-core/happy/prepy/main' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_1 } from '../../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_2 } from '../../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_3 } from '../../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_4 } from '../../../modules/nf-core/bcftools/reheader' workflow SMALL_GERMLINE_BENCHMARK { take: diff --git a/subworkflows/local/small_somatic_benchmark.nf b/subworkflows/local/small_somatic_benchmark/main.nf similarity index 91% rename from subworkflows/local/small_somatic_benchmark.nf rename to subworkflows/local/small_somatic_benchmark/main.nf index 27e36cc..6e9c220 100644 --- a/subworkflows/local/small_somatic_benchmark.nf +++ b/subworkflows/local/small_somatic_benchmark/main.nf @@ -2,8 +2,8 @@ // SOMATIC: SUBWORKFLOW FOR SMALL SOMATIC VARIANTS // -include { HAPPY_SOMPY } from '../../modules/nf-core/happy/sompy/main' -include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon/evaluator' +include { HAPPY_SOMPY } from '../../../modules/nf-core/happy/sompy/main' +include { BAMSURGEON_EVALUATOR } from '../../../modules/local/bamsurgeon/evaluator' workflow SMALL_SOMATIC_BENCHMARK { take: diff --git a/subworkflows/local/split_small_variants_test.nf b/subworkflows/local/split_small_variants_test/main.nf similarity index 84% rename from subworkflows/local/split_small_variants_test.nf rename to subworkflows/local/split_small_variants_test/main.nf index d595729..bc1a3be 100644 --- a/subworkflows/local/split_small_variants_test.nf +++ b/subworkflows/local/split_small_variants_test/main.nf @@ -2,10 +2,10 @@ // SPLIT_SMALL_VARIANTS_TEST: SUBWORKFLOW TO SPLIT SMALL SOMATIC VARIANTS INTO SNV AND INDEL // -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_SNV } from '../../modules/nf-core/bcftools/view' -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_INDEL } from '../../modules/nf-core/bcftools/view' -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_SNV } from '../../modules/nf-core/tabix/bgziptabix' -include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_INDEL } from '../../modules/nf-core/tabix/bgziptabix' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_SNV } from '../../../modules/nf-core/bcftools/view' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_INDEL } from '../../../modules/nf-core/bcftools/view' +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_SNV } from '../../../modules/nf-core/tabix/bgziptabix' +include { TABIX_BGZIPTABIX as TABIX_BGZIPTABIX_INDEL } from '../../../modules/nf-core/tabix/bgziptabix' workflow SPLIT_SMALL_VARIANTS_TEST { take: diff --git a/subworkflows/local/subsample_vcf_test.nf b/subworkflows/local/subsample_vcf_test/main.nf similarity index 88% rename from subworkflows/local/subsample_vcf_test.nf rename to subworkflows/local/subsample_vcf_test/main.nf index 958b891..8e1fa97 100644 --- a/subworkflows/local/subsample_vcf_test.nf +++ b/subworkflows/local/subsample_vcf_test/main.nf @@ -2,9 +2,9 @@ // SUBSAMPLE_VCF_TEST: SUBWORKFLOW TO PREPARE SUBSET SAMPLES // -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_SUBSAMPLE } from '../../modules/nf-core/bcftools/view' -include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_FILTERMISSING } from '../../modules/nf-core/bcftools/view' -include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_SUBSAMPLE } from '../../../modules/nf-core/bcftools/view' +include { BCFTOOLS_VIEW as BCFTOOLS_VIEW_FILTERMISSING } from '../../../modules/nf-core/bcftools/view' +include { BCFTOOLS_SORT } from '../../../modules/nf-core/bcftools/sort' workflow SUBSAMPLE_VCF_TEST { diff --git a/subworkflows/local/sv_germline_benchmark.nf b/subworkflows/local/sv_germline_benchmark/main.nf similarity index 92% rename from subworkflows/local/sv_germline_benchmark.nf rename to subworkflows/local/sv_germline_benchmark/main.nf index 1ad0b3b..a16ab75 100644 --- a/subworkflows/local/sv_germline_benchmark.nf +++ b/subworkflows/local/sv_germline_benchmark/main.nf @@ -2,15 +2,15 @@ // SV_GERMLINE_BENCHMARK: SUBWORKFLOW FOR SV GERMLINE VARIANTS // -include { TRUVARI_BENCH } from '../../modules/nf-core/truvari/bench' -include { SVANALYZER_SVBENCHMARK } from '../../modules/nf-core/svanalyzer/svbenchmark' -include { WITTYER } from '../../modules/nf-core/wittyer' -include { TABIX_BGZIP as TABIX_BGZIP_QUERY } from '../../modules/nf-core/tabix/bgzip' -include { TABIX_BGZIP as TABIX_BGZIP_TRUTH } from '../../modules/nf-core/tabix/bgzip' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_1 } from '../../modules/nf-core/bcftools/reheader' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_2 } from '../../modules/nf-core/bcftools/reheader' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_3 } from '../../modules/nf-core/bcftools/reheader' -include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_4 } from '../../modules/nf-core/bcftools/reheader' +include { TRUVARI_BENCH } from '../../../modules/nf-core/truvari/bench' +include { SVANALYZER_SVBENCHMARK } from '../../../modules/nf-core/svanalyzer/svbenchmark' +include { WITTYER } from '../../../modules/nf-core/wittyer' +include { TABIX_BGZIP as TABIX_BGZIP_QUERY } from '../../../modules/nf-core/tabix/bgzip' +include { TABIX_BGZIP as TABIX_BGZIP_TRUTH } from '../../../modules/nf-core/tabix/bgzip' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_1 } from '../../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_2 } from '../../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_3 } from '../../../modules/nf-core/bcftools/reheader' +include { BCFTOOLS_REHEADER as BCFTOOLS_REHEADER_4 } from '../../../modules/nf-core/bcftools/reheader' workflow SV_GERMLINE_BENCHMARK { take: diff --git a/subworkflows/local/sv_somatic_benchmark.nf b/subworkflows/local/sv_somatic_benchmark/main.nf similarity index 92% rename from subworkflows/local/sv_somatic_benchmark.nf rename to subworkflows/local/sv_somatic_benchmark/main.nf index 015f69b..65bae27 100644 --- a/subworkflows/local/sv_somatic_benchmark.nf +++ b/subworkflows/local/sv_somatic_benchmark/main.nf @@ -2,7 +2,7 @@ // SOMATIC: SUBWORKFLOW FOR SMALL SOMATIC VARIANTS // -include { BAMSURGEON_EVALUATOR } from '../../modules/local/bamsurgeon/evaluator' +include { BAMSURGEON_EVALUATOR } from '../../../modules/local/bamsurgeon/evaluator' workflow SV_SOMATIC_BENCHMARK { take: diff --git a/subworkflows/local/sv_vcf_conversion.nf b/subworkflows/local/sv_vcf_conversion/main.nf similarity index 88% rename from subworkflows/local/sv_vcf_conversion.nf rename to subworkflows/local/sv_vcf_conversion/main.nf index a236f36..0c9af4b 100644 --- a/subworkflows/local/sv_vcf_conversion.nf +++ b/subworkflows/local/sv_vcf_conversion/main.nf @@ -4,10 +4,10 @@ import groovy.io.FileType // SV_VCF_CONVERSIONS: SUBWORKFLOW to apply tool spesific conversions // -include { SVYNC } from '../../modules/nf-core/svync' -include { BGZIP_TABIX } from '../../modules/local/bgzip/tabix' -include { VARIANT_EXTRACTOR } from '../../modules/local/custom/variant_extractor' -include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' +include { SVYNC } from '../../../modules/nf-core/svync' +include { BGZIP_TABIX } from '../../../modules/local/bgzip/tabix' +include { VARIANT_EXTRACTOR } from '../../../modules/local/custom/variant_extractor' +include { BCFTOOLS_SORT } from '../../../modules/nf-core/bcftools/sort' workflow SV_VCF_CONVERSIONS { take: diff --git a/subworkflows/local/vcf_variant_deduplication.nf b/subworkflows/local/vcf_variant_deduplication/main.nf similarity index 81% rename from subworkflows/local/vcf_variant_deduplication.nf rename to subworkflows/local/vcf_variant_deduplication/main.nf index b6331e1..34d8b45 100644 --- a/subworkflows/local/vcf_variant_deduplication.nf +++ b/subworkflows/local/vcf_variant_deduplication/main.nf @@ -2,8 +2,8 @@ // VCF_VARIANT_DEDUPLICATION: deduplicate, sort and index vcf files // -include { BCFTOOLS_SORT } from '../../modules/nf-core/bcftools/sort' -include { BCFTOOLS_NORM as BCFTOOLS_DEDUP } from '../../modules/nf-core/bcftools/norm' +include { BCFTOOLS_SORT } from '../../../modules/nf-core/bcftools/sort' +include { BCFTOOLS_NORM as BCFTOOLS_DEDUP } from '../../../modules/nf-core/bcftools/norm' workflow VCF_VARIANT_DEDUPLICATION { take: diff --git a/subworkflows/local/vcf_variant_filtering.nf b/subworkflows/local/vcf_variant_filtering/main.nf similarity index 84% rename from subworkflows/local/vcf_variant_filtering.nf rename to subworkflows/local/vcf_variant_filtering/main.nf index 5cba729..dfd58d4 100644 --- a/subworkflows/local/vcf_variant_filtering.nf +++ b/subworkflows/local/vcf_variant_filtering/main.nf @@ -2,10 +2,10 @@ // VCF_VARIANT_FILTERING: Filter SV using survivor and bcftools // -include { SURVIVOR_FILTER } from '../../modules/nf-core/survivor/filter' -include { TABIX_BGZIP } from '../../modules/nf-core/tabix/bgzip' -include { TABIX_BGZIPTABIX } from '../../modules/nf-core/tabix/bgziptabix' -include { BCFTOOLS_FILTER } from '../../modules/nf-core/bcftools/filter' +include { SURVIVOR_FILTER } from '../../../modules/nf-core/survivor/filter' +include { TABIX_BGZIP } from '../../../modules/nf-core/tabix/bgzip' +include { TABIX_BGZIPTABIX } from '../../../modules/nf-core/tabix/bgziptabix' +include { BCFTOOLS_FILTER } from '../../../modules/nf-core/bcftools/filter' workflow VCF_VARIANT_FILTERING { From 80e9a3cf6d5699eb9ccbade01a7f1129d19a1ee8 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 12:11:12 +0000 Subject: [PATCH 257/292] fix description --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 200178a..075add9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -287,7 +287,7 @@ manifest { ], ] homePage = 'https://github.com/nf-core/variantbenchmarking' - description = """Benchmarking pipeline for germline and somatic variant callers """ + description = """Variant Benchmarking pipeline for germline and somatic variant callers """ mainScript = 'main.nf' defaultBranch = 'master' nextflowVersion = '!>=24.04.2' From 780b993cf28e890560601e9ac4db57c7446071fe Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 12:16:03 +0000 Subject: [PATCH 258/292] fix email_template --- assets/email_template.html | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/email_template.html b/assets/email_template.html index de8972a..209ef9a 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -4,7 +4,7 @@ - + nf-core/variantbenchmarking Pipeline Report From 2480fc0a2051ae42ce3f035f1add6b63bbfd9e15 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 12:31:05 +0000 Subject: [PATCH 259/292] add nf-test and plugins --- CITATIONS.md | 8 ++++++++ 1 file changed, 8 insertions(+) diff --git a/CITATIONS.md b/CITATIONS.md index c37ef6f..ca1a0f5 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -8,6 +8,14 @@ > Di Tommaso P, Chatzou M, Floden EW, Barja PP, Palumbo E, Notredame C. Nextflow enables reproducible computational workflows. Nat Biotechnol. 2017 Apr 11;35(4):316-319. doi: 10.1038/nbt.3820. PubMed PMID: 28398311. +## [nf-test](https://doi.org/10.1101/2024.05.25.595877) + +> Forer, L., & Schönherr, S. (2024). Improving the Reliability and Quality of Nextflow Pipelines with nf-test. bioRxiv. https://doi.org/10.1101/2024.05.25.595877 + +## [nf-prov](https://github.com/nextflow-io/nf-prov) + +## [nf-schema](https://nextflow-io.github.io/nf-schema/latest/) + ## Pipeline tools - [Bcftools](http://samtools.github.io/bcftools/bcftools.html) From 16e87f5745e50e43d2eca2499d02201bfe70f4ea Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 12:51:27 +0000 Subject: [PATCH 260/292] adding new logos and metromap --- docs/abstracts/ESCB_2024_kubra.pdf | Bin 0 -> 70250 bytes ...nf-core-variant_benchmarking_logo_dark.png | Bin 0 -> 89328 bytes ...f-core-variant_benchmarking_logo_light.png | Bin 0 -> 126515 bytes .../nf-core-variant_benchmarking_sticker.png | Bin 0 -> 80494 bytes .../nf-core-variant_benchmarking_sticker.svg | 286 ++ .../nf-core-variantbenchmarking_logo_dark.png | Bin 21301 -> 0 bytes ...nf-core-variantbenchmarking_logo_light.png | Bin 18104 -> 0 bytes docs/images/variantbenchmarking_metromap.png | Bin 239288 -> 290453 bytes docs/images/variantbenchmarking_metromap.svg | 3715 +++++++++++++++++ 9 files changed, 4001 insertions(+) create mode 100644 docs/abstracts/ESCB_2024_kubra.pdf create mode 100644 docs/images/nf-core-variant_benchmarking_logo_dark.png create mode 100644 docs/images/nf-core-variant_benchmarking_logo_light.png create mode 100644 docs/images/nf-core-variant_benchmarking_sticker.png create mode 100644 docs/images/nf-core-variant_benchmarking_sticker.svg delete mode 100644 docs/images/nf-core-variantbenchmarking_logo_dark.png delete mode 100644 docs/images/nf-core-variantbenchmarking_logo_light.png create mode 100644 docs/images/variantbenchmarking_metromap.svg diff --git a/docs/abstracts/ESCB_2024_kubra.pdf b/docs/abstracts/ESCB_2024_kubra.pdf new file mode 100644 index 0000000000000000000000000000000000000000..a609d32be1ac649eb4a3be5bed4542a9b454c2d4 GIT binary patch literal 70250 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variantbenchmarking From 03dfb838a561af54874fafe9746ac2bbec8fb2ee Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 13:16:07 +0000 Subject: [PATCH 263/292] rename images --- ...ng => nf-core-variantbenchmarking_logo_dark.png} | Bin ...g => nf-core-variantbenchmarking_logo_light.png} | Bin ....png => nf-core-variantbenchmarking_sticker.png} | Bin ....svg => nf-core-variantbenchmarking_sticker.svg} | 0 4 files changed, 0 insertions(+), 0 deletions(-) rename docs/images/{nf-core-variant_benchmarking_logo_dark.png => nf-core-variantbenchmarking_logo_dark.png} (100%) rename docs/images/{nf-core-variant_benchmarking_logo_light.png => nf-core-variantbenchmarking_logo_light.png} (100%) rename docs/images/{nf-core-variant_benchmarking_sticker.png => nf-core-variantbenchmarking_sticker.png} (100%) rename docs/images/{nf-core-variant_benchmarking_sticker.svg => nf-core-variantbenchmarking_sticker.svg} (100%) diff --git a/docs/images/nf-core-variant_benchmarking_logo_dark.png b/docs/images/nf-core-variantbenchmarking_logo_dark.png similarity index 100% rename from docs/images/nf-core-variant_benchmarking_logo_dark.png rename to docs/images/nf-core-variantbenchmarking_logo_dark.png diff --git a/docs/images/nf-core-variant_benchmarking_logo_light.png b/docs/images/nf-core-variantbenchmarking_logo_light.png similarity index 100% rename from docs/images/nf-core-variant_benchmarking_logo_light.png rename to docs/images/nf-core-variantbenchmarking_logo_light.png diff --git a/docs/images/nf-core-variant_benchmarking_sticker.png b/docs/images/nf-core-variantbenchmarking_sticker.png similarity index 100% rename from docs/images/nf-core-variant_benchmarking_sticker.png rename to docs/images/nf-core-variantbenchmarking_sticker.png diff --git a/docs/images/nf-core-variant_benchmarking_sticker.svg b/docs/images/nf-core-variantbenchmarking_sticker.svg similarity index 100% rename from docs/images/nf-core-variant_benchmarking_sticker.svg rename to docs/images/nf-core-variantbenchmarking_sticker.svg From 90902d8b8e1df0f81df4b3fe30cca372e1a2881a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 13:20:10 +0000 Subject: [PATCH 264/292] ignore lint error for logos --- .nf-core.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 63c7b08..c7823b7 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,6 +2,8 @@ lint: actions_ci: false files_unchanged: - .gitignore + - docs/images/nf-core-variantbenchmarking_logo_dark.png + - docs/images/nf-core-variantbenchmarking_logo_light.png nf_core_version: 3.2.0 repository_type: pipeline template: From eafffa8ee52d46d5abc526a7611b6835c0999454 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 13:54:38 +0000 Subject: [PATCH 265/292] higher quality --- .../nf-core-variantbenchmarking_logo_dark.png | Bin 89328 -> 258882 bytes 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a/docs/images/variantbenchmarking_metromap.svg +++ b/docs/images/variantbenchmarking_metromap.svg @@ -2,9 +2,9 @@ benchmarkingpicard CreateSequenceDictionaryUCSCliftoverpicard LiftoverVcfvariant extractorsvyncbcftools mergeSURVIVORmergeVCF to CSVmerge summarystatsdatavzrdplotsummarystatssompyhappyrtgtools matchtruth /test matchreportstruvariwittyersvbenchmarkfilter contigsbcftools filterSURVIVORfilterfilterbcftools statsSURVIVORstatsbcftools reheader TEST VCFsMulti-sample Single-sample Test preprocessingBenchmarkingOutput processingStructural variants onlyAll variantsLEGENDOptional processesSmall variants onlySomatic variants onlyNextflow functionTruth preprocessingReferencesbcftools normalizationprepybcftools split SNVs and INDELsbcftools deduplicationbcftools split multi-allelicsvariant variant filteringfilteringvariant statisticsvariant preprocessing--methodvariantstandardizationvariantliftovervariant liftoversummary reportingchainindexfastafastachainindexchromosomprefixeschromosomprefixestxttxtchainchainreformatingreformatingrulesUCSCliftover Date: Thu, 30 Jan 2025 15:04:03 +0000 Subject: [PATCH 268/292] replace svg metromap --- docs/images/variantbenchmarking_metromap.svg | 1321 +++--------------- 1 file changed, 162 insertions(+), 1159 deletions(-) diff --git a/docs/images/variantbenchmarking_metromap.svg b/docs/images/variantbenchmarking_metromap.svg index 4040ed7..0a17f83 100644 --- a/docs/images/variantbenchmarking_metromap.svg +++ b/docs/images/variantbenchmarking_metromap.svg @@ -8,87 +8,51 @@ version="1.1" id="svg1" xml:space="preserve" - inkscape:version="1.3.2 (091e20e, 2023-11-25)" - sodipodi:docname="variantbenchmarking_metromap.v1.1.0.svg" - inkscape:export-filename="variantbenchmarking_metromap.v1.1.0.png" - inkscape:export-xdpi="300" - inkscape:export-ydpi="300" - xmlns:inkscape="http://www.inkscape.org/namespaces/inkscape" - xmlns:sodipodi="http://sodipodi.sourceforge.net/DTD/sodipodi-0.dtd" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.w3.org/2000/svg" - xmlns:svg="http://www.w3.org/2000/svg"> + style="fill:#fcf1f9"> + style="fill:#000000"> + style="fill:#000000"> + style="fill:#000000"> + style="fill:#000000"> + style="fill:#000000"> + style="fill:#000000"> picard CreateSequencepicard variant bcftools SURVIVORVCF to CSVmerge summarystatsdatavzrdplotsummarystats match matchfilter bcftools SURVIVORfilterbcftools SURVIVORbcftools TEST VCFsBenchmarkingTruth preprocessingReferencesbcftools bcftools bcftools bcftools variant filteringvariant statisticsvariant preprocessing--methodvariantstandardizationvariant liftoversummary reportingTRUTHHigh Confidence chainchromosomrtg-tools sdfreformatingrulesstatsstatsUCSC Date: Thu, 30 Jan 2025 15:05:22 +0000 Subject: [PATCH 269/292] svg --- .../variantbenchmarking_metromap.v1.1.000.svg | 2727 +++++++++++++++++ 1 file changed, 2727 insertions(+) create mode 100644 docs/images/variantbenchmarking_metromap.v1.1.000.svg diff --git a/docs/images/variantbenchmarking_metromap.v1.1.000.svg b/docs/images/variantbenchmarking_metromap.v1.1.000.svg new file mode 100644 index 0000000..0a17f83 --- /dev/null +++ b/docs/images/variantbenchmarking_metromap.v1.1.000.svg @@ -0,0 +1,2727 @@ + + + + + + + + + + + + + + + + + + benchmarkingpicard CreateSequenceDictionarypicard LiftoverVcfvariant extractorsvyncbcftools mergeSURVIVORmergeVCF to CSVmerge summarystatsdatavzrdplotsummarystatssompyhappyrtgtools matchtruth /test matchreportstruvariwittyersvbenchmarkfilter contigsbcftools filterSURVIVORfilterbcftools statsSURVIVORstatsbcftools reheader TEST VCFsMulti-sample Single-sample Test preprocessingBenchmarkingOutput processingStructural variants onlyAll variantsLEGENDOptional processesSmall variants onlySomatic variants onlyNextflow functionTruth preprocessingReferencesbcftools normalizationprepybcftools split SNVs and INDELsbcftools deduplicationbcftools split multi-allelicsvariant filteringvariant statisticsvariant preprocessing--methodvariantstandardizationvariant liftoversummary reportingTRUTH VCFs High Confidence Regionsgenomebedvcffastachainindexchromosomprefixestxtchainrtg-tools sdfsdfreformatingrulesyamlstatsrochtmlpngcsvvcfstatsUCSCliftoverv1.0.0 From dd5b9731a29c2eb8c03b334da7ed42510715f1bc Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 30 Jan 2025 15:10:32 +0000 Subject: [PATCH 270/292] delete svg --- docs/images/variantbenchmarking_metromap.svg | 2727 ----------------- .../variantbenchmarking_metromap.v1.1.000.svg | 2727 ----------------- 2 files changed, 5454 deletions(-) delete mode 100644 docs/images/variantbenchmarking_metromap.svg delete mode 100644 docs/images/variantbenchmarking_metromap.v1.1.000.svg diff --git a/docs/images/variantbenchmarking_metromap.svg b/docs/images/variantbenchmarking_metromap.svg deleted file mode 100644 index 0a17f83..0000000 --- a/docs/images/variantbenchmarking_metromap.svg +++ /dev/null @@ -1,2727 +0,0 @@ - - - - - - - - - - - - - - - - - - benchmarkingpicard CreateSequenceDictionarypicard LiftoverVcfvariant extractorsvyncbcftools mergeSURVIVORmergeVCF to CSVmerge summarystatsdatavzrdplotsummarystatssompyhappyrtgtools matchtruth /test matchreportstruvariwittyersvbenchmarkfilter contigsbcftools filterSURVIVORfilterbcftools statsSURVIVORstatsbcftools reheader TEST VCFsMulti-sample Single-sample Test preprocessingBenchmarkingOutput processingStructural variants onlyAll variantsLEGENDOptional processesSmall variants onlySomatic variants onlyNextflow functionTruth preprocessingReferencesbcftools normalizationprepybcftools split SNVs and INDELsbcftools deduplicationbcftools split multi-allelicsvariant filteringvariant statisticsvariant preprocessing--methodvariantstandardizationvariant liftoversummary reportingTRUTH VCFs High Confidence Regionsgenomebedvcffastachainindexchromosomprefixestxtchainrtg-tools 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+0000 Subject: [PATCH 273/292] adding full size aws tests --- .github/workflows/awsfulltest.yml | 26 +- assets/samplesheet.csv | 7 +- conf/test.config | 16 +- ..._full.config => test_full_germline.config} | 4 +- conf/test_full_somatic.config | 37 +++ conf/test_hg37.config | 45 --- conf/tests/germline_small.config | 6 +- conf/tests/germline_sv.config | 6 +- conf/tests/liftover_test.config | 8 +- conf/tests/liftover_truth.config | 8 +- conf/tests/somatic_indel.config | 6 +- conf/tests/somatic_snv.config | 6 +- conf/tests/somatic_sv.config | 12 +- nextflow.config | 6 +- nextflow_schema.json | 7 + tests/nextflow.config | 5 +- tests/test.nf.test | 70 ++++ tests/test.nf.test.snap | 298 ++++++++++++++++++ 18 files changed, 480 insertions(+), 93 deletions(-) rename conf/{test_full.config => test_full_germline.config} (89%) create mode 100644 conf/test_full_somatic.config delete mode 100644 conf/test_hg37.config create mode 100644 tests/test.nf.test create mode 100644 tests/test.nf.test.snap diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 9b0eeaf..d2bf70c 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -9,11 +9,34 @@ on: - main - master workflow_dispatch: + inputs: + germline: + description: "Germline full test" + type: boolean + default: true + somatic: + description: "Somatic full test" + type: boolean + default: false pull_request_review: types: [submitted] jobs: run-platform: + strategy: + fail-fast: false + matrix: + include: + - profile: test_full_germline + test: germline + cloud: aws + compute_env: TOWER_COMPUTE_ENV + workdir: TOWER_BUCKET_AWS + - profile: test_full_somatic + test: somatic + cloud: aws + compute_env: TOWER_COMPUTE_ENV + workdir: TOWER_BUCKET_AWS name: Run AWS full tests # run only if the PR is approved by at least 2 reviewers and against the master branch or manually triggered if: github.repository == 'nf-core/variantbenchmarking' && github.event.review.state == 'approved' && github.event.pull_request.base.ref == 'master' || github.event_name == 'workflow_dispatch' @@ -49,6 +72,7 @@ jobs: # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters with: + run_name: variantbenchmarking_${{ matrix.profile }} workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} @@ -59,7 +83,7 @@ jobs: "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}", "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/variantbenchmarking/results-${{ github.sha }}" } - profiles: test_full + profiles: ${{ matrix.profile }} - uses: actions/upload-artifact@v4 with: diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv index 9ed0261..7ba5829 100644 --- a/assets/samplesheet.csv +++ b/assets/samplesheet.csv @@ -1,5 +1,4 @@ id,test_vcf,caller -test1,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/HG002_delly_SV_hg19.chr21.vcf.gz,delly -test2,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/HG002_lumpy_SV_hg19.sorted.chr21.vcf.gz,lumpy -test3,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/HG002_manta_SV_hg19_genotype2.chr21.vcf.gz,manta -test4,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/full.svaba.germline.sv.chr21.vcf.gz,svaba +test1,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/germline/HG002_delly_SV_hg19.chr21.vcf.gz,delly +test2,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/germline/HG002_lumpy_SV_hg19.sorted.vcf.gz,lumpy +test3,https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/test/germline/HG002_manta_SV_hg19_genotype.chr21.vcf.gz,manta diff --git a/conf/test.config b/conf/test.config index 14a963b..226cab9 100644 --- a/conf/test.config +++ b/conf/test.config @@ -23,23 +23,21 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_small_germline_hg38.csv" + input = "${projectDir}/assets/samplesheet.csv" outdir = "results" // Genome references - genome = 'GRCh38' + genome = 'GRCh37' // Processes analysis = 'germline' - variant_type = "small" - method = 'happy,rtgtools' - preprocess = "normalize,deduplicate,prepy" - + variant_type = "structural" + method = 'svanalyzer' + preprocess = "normalize,deduplicate,filter_contigs" // truth information truth_id = "HG002" - - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/HG002_GRCh38_CMRG_smallvar_v1.00.bed" + truth_vcf = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.bed" } diff --git a/conf/test_full.config b/conf/test_full_germline.config similarity index 89% rename from conf/test_full.config rename to conf/test_full_germline.config index 1933f25..eec0890 100644 --- a/conf/test_full.config +++ b/conf/test_full_germline.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = 'assets/samplesheet_full_small.csv' + input = "${params.test_data_base}/samplesheet/samplesheet_full_small.csv" outdir = 'results' // Genome references @@ -28,7 +28,7 @@ params { analysis = 'germline' variant_type = "small" method = 'happy,rtgtools' - preprocess = "normalize,deduplicate,filter_contigs" + preprocess = "split_multiallelic,normalize,deduplicate,filter_contigs" truth_id = "HG002" truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/giab/release/AshkenazimTrio/HG002_NA24385_son/NISTv4.2.1/GRCh37/SupplementaryFiles/HG002_GRCh37_1_22_v4.2.1_highconf.vcf.gz" diff --git a/conf/test_full_somatic.config b/conf/test_full_somatic.config new file mode 100644 index 0000000..27dcf18 --- /dev/null +++ b/conf/test_full_somatic.config @@ -0,0 +1,37 @@ +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Nextflow config file for running minimal tests +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + Defines input files and everything required to run a full size pipeline test. + + Use as follows: + nextflow run nf-core/variantbenchmarking -profile test_full, + +---------------------------------------------------------------------------------------- +*/ + +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 16 + max_memory = 100.GB + max_time = '8.h' + + // Input data + input = "${params.test_data_base}/samplesheet/samplesheet_full_somatic.csv" + outdir = 'results' + + // Genome references + genome = 'GRCh38' + analysis = 'somatic' + variant_type = "indel" + method = 'sompy' + preprocess = "split_multiallelic,normalize,deduplicate,filter_contigs" + + truth_id = "SEQC2" + truth_vcf = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/v1.1/sINDEL.MDKT.superSet.v1.1.vcf.gz" + regions_bed = "https://ftp-trace.ncbi.nlm.nih.gov/ReferenceSamples/seqc/Somatic_Mutation_WG/release/v1.1/High-Confidence_Regions.bed" + +} diff --git a/conf/test_hg37.config b/conf/test_hg37.config deleted file mode 100644 index 45b5041..0000000 --- a/conf/test_hg37.config +++ /dev/null @@ -1,45 +0,0 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Nextflow config file for running minimal tests -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - Defines input files and everything required to run a fast and simple pipeline test. - - Use as follows: - nextflow run nf-core/variantbenchmarking -profile test, --outdir - ----------------------------------------------------------------------------------------- -*/ - -process { - resourceLimits = [ - cpus: 4, - memory: '15.GB', - time: '1.h' - ] -} - -params { - config_profile_name = 'Test profile' - config_profile_description = 'Minimal test dataset to check pipeline function' - - // Input data - input = "assets/samplesheet.csv" - - outdir = "results" - - // Genome references - genome = 'GRCh37' - - // Processes - analysis = 'germline' - variant_type = "structural" - method = 'truvari,svanalyzer' - preprocess = "normalize,deduplicate,filter_contigs" - - - // truth information - truth_id = "HG002" - - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/HG002_SVs_Tier1_v0.6.chr21.bed" -} diff --git a/conf/tests/germline_small.config b/conf/tests/germline_small.config index 9b98222..ec71b09 100644 --- a/conf/tests/germline_small.config +++ b/conf/tests/germline_small.config @@ -23,7 +23,7 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_small_germline_hg38.csv" + input = "${params.test_data_base}/samplesheet/samplesheet_small_germline_hg38.csv" outdir = "results" // Genome references @@ -38,7 +38,7 @@ params { // truth information truth_id = "HG002" - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_CMRG_smallvar_v1.00.bed" + truth_vcf = "${params.test_data_base}/testdata/hg38/truth/germline/HG002_GRCh38_CMRG_smallvar_v1.00.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg38/truth/germline/HG002_GRCh38_CMRG_smallvar_v1.00.bed" rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config index 5128134..7af553a 100644 --- a/conf/tests/germline_sv.config +++ b/conf/tests/germline_sv.config @@ -23,7 +23,7 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_sv_germline_hg38.csv" + input = "${params.test_data_base}/samplesheet/samplesheet_sv_germline_hg38.csv" outdir = "results" // Genome references @@ -39,7 +39,7 @@ params { truth_id = "HG002" include_expression = 'FILTER="."' - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" + truth_vcf = "${params.test_data_base}/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/liftover_test.config b/conf/tests/liftover_test.config index cbca43e..c044c94 100644 --- a/conf/tests/liftover_test.config +++ b/conf/tests/liftover_test.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile liftover_hg37, --outdir + nextflow run nf-core/variantbenchmarking -profile liftover_test, --outdir ---------------------------------------------------------------------------------------- */ @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_sv_germline_hg37_liftover.csv" + input = "${params.test_data_base}/samplesheet/samplesheet_sv_germline_hg37_liftover.csv" outdir = 'results' genome = 'GRCh37' @@ -32,8 +32,8 @@ params { min_sv_size = 30 truth_id = "HG002" - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.bed" + truth_vcf = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.bed" //liftover files liftover = "test" diff --git a/conf/tests/liftover_truth.config b/conf/tests/liftover_truth.config index 91b3eec..4052b10 100644 --- a/conf/tests/liftover_truth.config +++ b/conf/tests/liftover_truth.config @@ -5,7 +5,7 @@ Defines input files and everything required to run a fast and simple pipeline test. Use as follows: - nextflow run nf-core/variantbenchmarking -profile liftover_hg38, --outdir + nextflow run nf-core/variantbenchmarking -profile liftover_truth, --outdir ---------------------------------------------------------------------------------------- */ @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_small_germline_hg38.csv" + input = "${params.test_data_base}/samplesheet/samplesheet_small_germline_hg38.csv" outdir = 'results' genome = 'GRCh38' @@ -31,8 +31,8 @@ params { method = 'rtgtools,happy' preprocess = "split_multiallelic,deduplicate,filter_contigs,normalize" - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/germline/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg37/truth/germline/HG002_GRCh37_1_22_v4.2.1_highconf.bed" + truth_vcf = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_GRCh37_1_22_v4.2.1_highconf.chr21.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_GRCh37_1_22_v4.2.1_highconf.bed" //liftover files liftover = "truth" diff --git a/conf/tests/somatic_indel.config b/conf/tests/somatic_indel.config index 0644139..58f7306 100644 --- a/conf/tests/somatic_indel.config +++ b/conf/tests/somatic_indel.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_indel_somatic_hg38.csv" + input = "${params.test_data_base}/samplesheet/samplesheet_indel_somatic_hg38.csv" outdir = 'results' @@ -32,7 +32,7 @@ params { preprocess = "filter_contigs" variant_type = "indel" - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/somatic/sINDEL_truth_set_v1.0.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/somatic/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" + truth_vcf = "${params.test_data_base}/testdata/hg38/truth/somatic/sINDEL_truth_set_v1.0.chr21.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg38/truth/somatic/high-confidence_sINDEL_in_HC_regions_v1.2.chr21.vcf.gz" rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/somatic_snv.config b/conf/tests/somatic_snv.config index ba590e5..d3eb827 100644 --- a/conf/tests/somatic_snv.config +++ b/conf/tests/somatic_snv.config @@ -20,7 +20,7 @@ params { max_time = '8.h' // Input data - input = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_snv_somatic_hg38.csv" + input = "${params.test_data_base}/samplesheet/samplesheet_snv_somatic_hg38.csv" outdir = 'results' // Genome references @@ -31,7 +31,7 @@ params { variant_type = "snv" truth_id = "SEQC2" - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/somatic/sSNV_truth_set_v1.0.chr21.vcf.gz" - regions_bed = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/somatic/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" + truth_vcf = "${params.test_data_base}/testdata/hg38/truth/somatic/sSNV_truth_set_v1.0.chr21.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg38/truth/somatic/high-confidence_sSNV_in_HC_regions_v1.2.chr21.vcf.gz" rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" } diff --git a/conf/tests/somatic_sv.config b/conf/tests/somatic_sv.config index 9b95991..d3cbde3 100644 --- a/conf/tests/somatic_sv.config +++ b/conf/tests/somatic_sv.config @@ -20,18 +20,18 @@ params { max_time = '8.h' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/samplesheet/samplesheet_sv_somatic_hg38.csv' - outdir = 'results' + input = "${params.test_data_base}/samplesheet/samplesheet_sv_somatic_hg38.csv" + outdir = "results" // Genome references - genome = 'GRCh38' - analysis = 'somatic' + genome = "GRCh38" + analysis = "somatic" variant_type = "structural" - method = 'truvari' + method = "truvari" preprocess = "filter_contigs" truth_id = "SEQC2" - truth_vcf = "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking/testdata/hg38/truth/somatic/13059_2022_2816_MOESM4_ESM.chr21.vcf.gz" + truth_vcf = "${params.test_data_base}/testdata/hg38/truth/somatic/13059_2022_2816_MOESM4_ESM.chr21.vcf.gz" regions_bed ="" rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" diff --git a/nextflow.config b/nextflow.config index 075add9..cb22cb3 100644 --- a/nextflow.config +++ b/nextflow.config @@ -79,6 +79,8 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + // Schema validation default options validate_params = true @@ -193,7 +195,6 @@ profiles { } } test { includeConfig 'conf/test.config' } - test_full { includeConfig 'conf/test_full.config' } germline_small { includeConfig 'conf/tests/germline_small.config'} germline_sv { includeConfig 'conf/tests/germline_sv.config' } somatic_snv { includeConfig 'conf/tests/somatic_snv.config' } @@ -201,7 +202,8 @@ profiles { somatic_sv { includeConfig 'conf/tests/somatic_sv.config' } liftover_test { includeConfig 'conf/tests/liftover_test.config' } liftover_truth { includeConfig 'conf/tests/liftover_truth.config' } - + test_germline_full { includeConfig 'conf/test_germline_full.config' } + test_somatic_full { includeConfig 'conf/test_somatic_full.config' } } // Load nf-core custom profiles from different Institutions diff --git a/nextflow_schema.json b/nextflow_schema.json index 0df663d..3859729 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -279,6 +279,13 @@ "description": "Institutional config URL link.", "hidden": true, "fa_icon": "fas fa-users-cog" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking", + "description": "Base path / URL for data used in the test profiles", + "help_text": "Warning: The `-profile test` samplesheet file itself contains remote paths. Setting this parameter does not alter the contents of that file.", + "hidden": true } } }, diff --git a/tests/nextflow.config b/tests/nextflow.config index 5b561b5..822f849 100644 --- a/tests/nextflow.config +++ b/tests/nextflow.config @@ -8,12 +8,9 @@ params { // Base directory for nf-core/modules test data modules_testdata_base_path = 's3://ngi-igenomes/testdata/nf-core/modules/' - // for hisat2 - validationSchemaIgnoreParams = 'genomes,igenomes_base,modules_testdata_base_path,pipelines_testdata_base_path' - - // TODO: check if we rather do this or disable publishdir for all processes when testing modules/subworkflows outdir = 'results' + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' } diff --git a/tests/test.nf.test b/tests/test.nf.test new file mode 100644 index 0000000..ff13fa2 --- /dev/null +++ b/tests/test.nf.test @@ -0,0 +1,70 @@ +nextflow_pipeline { + + name "Test pipeline for structural germline variants, tested benchmarking methods is svanalyzer" + script "../main.nf" + tag "pipeline" + tag "germline" + tag "structural" + config "../conf/test.config" + + test("Params: --analysis 'germline' --variant_type 'structural' --method 'svanalyzer'") { + + when { + params { + outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } + test("-stub") { + + options "-stub" + + when { + params { + outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } + } + + then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') + assertAll( + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } + ) + } + } +} diff --git a/tests/test.nf.test.snap b/tests/test.nf.test.snap new file mode 100644 index 0000000..b2c77f6 --- /dev/null +++ b/tests/test.nf.test.snap @@ -0,0 +1,298 @@ +{ + "Params: --analysis 'germline' --variant_type 'structural' --method 'svanalyzer'": { + "content": [ + 52, + null, + [ + "pipeline_info", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", + "structural", + "structural/HG002", + "structural/HG002/preprocess", + "structural/HG002/preprocess/HG002.vcf.gz", + "structural/HG002/preprocess/HG002.vcf.gz.tbi", + "structural/HG002/stats", + "structural/HG002/stats/bcftools", + 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"HG002_mqc.stats:md5,ba6a6398a99b95579872576cacde4c8c", + "test1.HG002.delly.distances:md5,a726347df41ebafd480e274105926606", + "test1.HG002.delly.report:md5,578be6e52289fba8a2949788d6c09d4f", + "test1.delly.bcftools_stats.txt:md5,209604ebf522660875a29723798e03ae", + "test1.delly_mqc.stats:md5,6e79c2fe59f011aedc63a6286ef85001", + "test2.HG002.lumpy.distances:md5,0f8f3a33cfaa22913b1e76596c6886f1", + "test2.HG002.lumpy.report:md5,2ce78dd8de9f8afcd4ed666a3c50f8aa", + "test2.lumpy.bcftools_stats.txt:md5,793655055cdb78556ae6b8e126ff743f", + "test2.lumpy_mqc.stats:md5,6a22796091a559398e14325a4349f19e", + "test3.HG002.manta.distances:md5,8bbecb45e074b416152b4b43fbad8878", + "test3.HG002.manta.report:md5,7931e8a4f87ea471d6128af55b50febb", + "test3.manta.bcftools_stats.txt:md5,a3f6a0e9aa7dbade8528836d3a2c8c85", + "test3.manta_mqc.stats:md5,c6d5b9f40c4aa8c7b30155464eb52e3c" + ] + ], + "meta": { + "nf-test": "0.9.2", + "nextflow": "24.10.4" + }, + "timestamp": "2025-01-31T15:19:18.268710518" + }, + "-stub": { + "content": [ + 52, + null, + [ + "pipeline_info", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", + "structural", + "structural/HG002", + "structural/HG002/preprocess", + "structural/HG002/preprocess/HG002.vcf.gz", + "structural/HG002/preprocess/HG002.vcf.gz.tbi", + "structural/HG002/stats", + "structural/HG002/stats/bcftools", + "structural/HG002/stats/bcftools/HG002.bcftools_stats.txt", + "structural/HG002/stats/survivor", + "structural/HG002/stats/survivor/HG002_mqc.stats", + "structural/multiqc", + "structural/multiqc/multiqc_data", + "structural/multiqc/multiqc_plots", + "structural/multiqc/multiqc_report.html", + "structural/summary", + "structural/summary/comparisons", + "structural/summary/comparisons/svbenchmark.FN.csv", + "structural/summary/comparisons/svbenchmark.FP.csv", + "structural/summary/datavzrd", + "structural/summary/datavzrd/svbenchmark", + "structural/summary/datavzrd/svbenchmark/index.html", + "structural/summary/datavzrd/svbenchmark/network", + "structural/summary/datavzrd/svbenchmark/network/config.js", + "structural/summary/datavzrd/svbenchmark/network/data", + "structural/summary/datavzrd/svbenchmark/network/data/data_1.js", + "structural/summary/datavzrd/svbenchmark/network/functions.js", + "structural/summary/datavzrd/svbenchmark/network/heatmap.js", + "structural/summary/datavzrd/svbenchmark/network/index_1.html", + "structural/summary/datavzrd/svbenchmark/network/plots", + "structural/summary/datavzrd/svbenchmark/network/plots/plot_0.js", + "structural/summary/datavzrd/svbenchmark/static", + "structural/summary/datavzrd/svbenchmark/static/bootstrap-select.min.css", + "structural/summary/datavzrd/svbenchmark/static/bootstrap-table-fixed-columns.min.css", + "structural/summary/datavzrd/svbenchmark/static/bootstrap-table.min.css", + "structural/summary/datavzrd/svbenchmark/static/bootstrap.min.css", + "structural/summary/datavzrd/svbenchmark/static/bundle.js", + "structural/summary/datavzrd/svbenchmark/static/datavzrd.css", + "structural/summary/datavzrd/versions.yml", + "structural/summary/plots", + "structural/summary/plots/svbenchmark", + "structural/summary/plots/svbenchmark/metric_by_tool_svbenchmark_mqc.png", + "structural/summary/plots/svbenchmark/variants_by_tool_svbenchmark_mqc.png", + "structural/summary/tables", + "structural/summary/tables/svbenchmark", + "structural/summary/tables/svbenchmark/svbenchmark.regions.csv", + "structural/summary/tables/svbenchmark/svbenchmark.summary.csv", + "structural/test1", + "structural/test1/benchmarks", + "structural/test1/benchmarks/svanalyzer", + "structural/test1/benchmarks/svanalyzer/test1.HG002.delly.distances", + "structural/test1/benchmarks/svanalyzer/test1.HG002.delly.falsenegatives.vcf.gz", + "structural/test1/benchmarks/svanalyzer/test1.HG002.delly.falsepositives.vcf.gz", + "structural/test1/benchmarks/svanalyzer/test1.HG002.delly.log", + "structural/test1/benchmarks/svanalyzer/test1.HG002.delly.report", + "structural/test1/preprocess", + "structural/test1/preprocess/test1.vcf.gz", + "structural/test1/preprocess/test1.vcf.gz.tbi", + "structural/test1/stats", + "structural/test1/stats/bcftools", + "structural/test1/stats/bcftools/test1.delly.bcftools_stats.txt", + "structural/test1/stats/survivor", + "structural/test1/stats/survivor/test1.delly_mqc.stats", + "structural/test2", + "structural/test2/benchmarks", + "structural/test2/benchmarks/svanalyzer", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.distances", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.falsenegatives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.falsepositives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.log", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.report", + "structural/test2/preprocess", + "structural/test2/preprocess/test2.vcf.gz", + "structural/test2/preprocess/test2.vcf.gz.tbi", + "structural/test2/stats", + "structural/test2/stats/bcftools", + "structural/test2/stats/bcftools/test2.lumpy.bcftools_stats.txt", + "structural/test2/stats/survivor", + "structural/test2/stats/survivor/test2.lumpy_mqc.stats", + "structural/test3", + "structural/test3/benchmarks", + "structural/test3/benchmarks/svanalyzer", + "structural/test3/benchmarks/svanalyzer/test3.HG002.manta.distances", + "structural/test3/benchmarks/svanalyzer/test3.HG002.manta.falsenegatives.vcf.gz", + "structural/test3/benchmarks/svanalyzer/test3.HG002.manta.falsepositives.vcf.gz", + "structural/test3/benchmarks/svanalyzer/test3.HG002.manta.log", + "structural/test3/benchmarks/svanalyzer/test3.HG002.manta.report", + "structural/test3/preprocess", + "structural/test3/preprocess/test3.vcf.gz", + "structural/test3/preprocess/test3.vcf.gz.tbi", + "structural/test3/stats", + "structural/test3/stats/bcftools", + "structural/test3/stats/bcftools/test3.manta.bcftools_stats.txt", + "structural/test3/stats/survivor", + "structural/test3/stats/survivor/test3.manta_mqc.stats" + ], + [ + "HG002.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "HG002_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "test1.HG002.delly.distances:md5,d41d8cd98f00b204e9800998ecf8427e", + "test1.HG002.delly.report:md5,d41d8cd98f00b204e9800998ecf8427e", + "test1.delly.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "test1.delly_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.HG002.lumpy.distances:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.HG002.lumpy.report:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.lumpy.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.lumpy_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "test3.HG002.manta.distances:md5,d41d8cd98f00b204e9800998ecf8427e", + "test3.HG002.manta.report:md5,d41d8cd98f00b204e9800998ecf8427e", + "test3.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "test3.manta_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "meta": { + "nf-test": "0.9.2", + "nextflow": "24.10.4" + }, + "timestamp": "2025-01-31T15:21:09.404351906" + } +} \ No newline at end of file From 3e804f8c9c85809b35319d8a43150d83562640ae Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 31 Jan 2025 15:27:26 +0000 Subject: [PATCH 274/292] test_full is named as test_full_germline and test_full_somatic --- .nf-core.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.nf-core.yml b/.nf-core.yml index c398cf4..8e80a8b 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,6 +2,7 @@ lint: actions_ci: false files_unchanged: - .gitignore + - test_full.config nf_core_version: 3.2.0 repository_type: pipeline template: From fe8620d0b6f4183492129788c886bbaa8aaf0159 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 31 Jan 2025 15:28:12 +0000 Subject: [PATCH 275/292] resolve conflicts --- .nf-core.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 8e80a8b..da4276d 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -2,6 +2,8 @@ lint: actions_ci: false files_unchanged: - .gitignore + - docs/images/nf-core-variantbenchmarking_logo_dark.png + - docs/images/nf-core-variantbenchmarking_logo_light.png - test_full.config nf_core_version: 3.2.0 repository_type: pipeline From 16c2689eeffa0bacdbeb7cd15ff887833a92a740 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 31 Jan 2025 15:31:40 +0000 Subject: [PATCH 276/292] correct path --- .nf-core.yml | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 32869e1..2090c55 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -4,8 +4,7 @@ lint: - .gitignore - docs/images/nf-core-variantbenchmarking_logo_dark.png - docs/images/nf-core-variantbenchmarking_logo_light.png - - test_full.config - + - conf/test_full.config nf_core_version: 3.2.0 repository_type: pipeline template: From aca8c9d9de66c11214ab02bad4723e2ceac943f4 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 31 Jan 2025 15:37:30 +0000 Subject: [PATCH 277/292] restore test_full --- .github/workflows/awsfulltest.yml | 6 +++--- .nf-core.yml | 1 - conf/{test_full_germline.config => test_full.config} | 0 3 files changed, 3 insertions(+), 4 deletions(-) rename conf/{test_full_germline.config => test_full.config} (100%) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index d2bf70c..64a2bf0 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -10,7 +10,7 @@ on: - master workflow_dispatch: inputs: - germline: + test_full: description: "Germline full test" type: boolean default: true @@ -27,8 +27,8 @@ jobs: fail-fast: false matrix: include: - - profile: test_full_germline - test: germline + - profile: test_full + test: test_full cloud: aws compute_env: TOWER_COMPUTE_ENV workdir: TOWER_BUCKET_AWS diff --git a/.nf-core.yml b/.nf-core.yml index 2090c55..c7823b7 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -4,7 +4,6 @@ lint: - .gitignore - docs/images/nf-core-variantbenchmarking_logo_dark.png - docs/images/nf-core-variantbenchmarking_logo_light.png - - conf/test_full.config nf_core_version: 3.2.0 repository_type: pipeline template: diff --git a/conf/test_full_germline.config b/conf/test_full.config similarity index 100% rename from conf/test_full_germline.config rename to conf/test_full.config From cc97ccdcbc0b88b8873288adaab5e1f73dc315da Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 3 Feb 2025 13:06:04 +0000 Subject: [PATCH 278/292] versions dump and release lints --- .nf-core.yml | 2 +- assets/multiqc_config.yml | 7 +-- nextflow.config | 6 +-- ro-crate-metadata.json | 95 ++++++++++++++++++++++++++++++++------- 4 files changed, 88 insertions(+), 22 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index c7823b7..2a25a63 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -14,4 +14,4 @@ template: name: variantbenchmarking org: nf-core outdir: . - version: 1.0dev + version: 1.0.0 diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 6b654de..7d09224 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,8 @@ report_comment: > - This report has been generated by the nf-core/variantbenchmarking - analysis pipeline. For information about how to interpret these results, please see the - documentation. + This report has been generated by the nf-core/variantbenchmarking analysis pipeline. For information + about how to interpret these results, please see the documentation. report_section_order: "nf-core-variantbenchmarking-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index cb22cb3..4a8045c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -210,7 +210,7 @@ profiles { includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/nfcore_custom.config" : "/dev/null" // Load nf-core/variantbenchmarking custom profiles from different institutions. -// includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/variantbenchmarking.config" : "/dev/null" +includeConfig !System.getenv('NXF_OFFLINE') && params.custom_config_base ? "${params.custom_config_base}/pipeline/variantbenchmarking.config" : "/dev/null" // Set default registry for Apptainer, Docker, Podman, Charliecloud and Singularity independent of -profile // Will not be used unless Apptainer / Docker / Podman / Charliecloud / Singularity are enabled @@ -284,7 +284,7 @@ manifest { affiliation: '', email: '', github: '', - contribution: [], // List of contribution types ('author', 'maintainer' or 'contributor') + contribution: ['author', 'maintainer'], // List of contribution types ('author', 'maintainer' or 'contributor') orcid: '' ], ] @@ -293,7 +293,7 @@ manifest { mainScript = 'main.nf' defaultBranch = 'master' nextflowVersion = '!>=24.04.2' - version = '1.0dev' + version = '1.0.0' doi = '' } diff --git a/ro-crate-metadata.json b/ro-crate-metadata.json index 4d6651d..845e0c7 100644 --- a/ro-crate-metadata.json +++ b/ro-crate-metadata.json @@ -21,9 +21,9 @@ { "@id": "./", "@type": "Dataset", - "creativeWorkStatus": "InProgress", - "datePublished": "2025-01-27T14:49:17+00:00", - "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    \n\n[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23variantbenchmarking-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is a bioinformatics pipeline that ...\n\n\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\n\n\nNow, you can run the pipeline using:\n\n\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by kuebra.narci@dkfz.de.\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", + "creativeWorkStatus": "Stable", + "datePublished": "2025-02-03T12:56:47+00:00", + "description": "

    \n \n \n \"nf-core/variantbenchmarking\"\n \n

    \n\n[![GitHub Actions CI Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/ci.yml)\n[![GitHub Actions Linting Status](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/variantbenchmarking/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/variantbenchmarking/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX)\n[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com)\n\n[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A524.04.2-23aa62.svg)](https://www.nextflow.io/)\n[![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/)\n[![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/)\n[![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/)\n[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/variantbenchmarking)\n\n[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23benchmark-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/variantbenchmarking)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core)\n\n## Introduction\n\n**nf-core/variantbenchmarking** is designed to evaluate and validate the accuracy of variant calling methods in genomic research. Initially, the pipeline is tuned well for available gold standard truth sets (for example, Genome in a Bottle and SEQC2 samples) but it can be used to compare any two variant calling results. The workflow provides benchmarking tools for small variants including SNVs and INDELs, Structural Variants (SVs) and Copy Number Variations (CNVs) for germline and somatic analysis.\n\nThe pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community!\n\n

    \n \n

    \n\nThe workflow involves several key processes to ensure reliable and reproducible results as follows:\n\n### Standardization and normalization of variants:\n\nThis initial step ensures consistent formatting and alignment of variants in test and truth VCF files for accurate comparison.\n\n1. Subsample if input test vcf is multisample ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view))\n2. Homogenization of multi-allelic variants, MNPs and SVs (including imprecise paired breakends and single breakends) ([variant-extractor](https://github.com/EUCANCan/variant-extractor))\n3. Reformatting test VCF files from different SV callers ([svync](https://github.com/nvnieuwk/svync))\n4. Rename sample names in test and truth VCF files ([bcftools reheader](https://samtools.github.io/bcftools/bcftools.html#reheader))\n5. Splitting multi-allelic variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm))\n6. Deduplication of variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm))\n7. Left aligning of variants in test and truth VCF files ([bcftools norm](https://samtools.github.io/bcftools/bcftools.html#norm))\n8. Use prepy in order to normalize test files. This option is only applicable for happy benchmarking of germline analysis ([prepy](https://github.com/Illumina/hap.py/tree/master))\n9. Split SNVs and indels if the given test VCF contains both. This is only applicable for somatic analysis ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view))\n\n### Filtering options:\n\nApplying filtering on the process of benchmarking itself might makes it impossible to compare different benchmarking strategies. Therefore, for whom like to compare benchmarking methods this subworkflow aims to provide filtering options for variants.\n\n10. Filtration of contigs ([bcftools view](https://samtools.github.io/bcftools/bcftools.html#view))\n11. Include or exclude SNVs and INDELs ([bcftools filter](https://samtools.github.io/bcftools/bcftools.html#filter))\n12. Size and quality filtering for SVs ([SURVIVOR filter](https://github.com/fritzsedlazeck/SURVIVOR/wiki))\n\n### Liftover of vcfs:\n\nThis sub-workflow provides option to convert genome coordinates of truth VCF and test VCFs and high confidence BED file to a new assembly. Golden standard truth files are build upon specific reference genomes which makes the necessity of lifting over depending on the test VCF in query. Lifting over one or more test VCFs is also possible.\n\n13. Create sequence dictionary for the reference ([picard CreateSequenceDictionary](https://gatk.broadinstitute.org/hc/en-us/articles/360037068312-CreateSequenceDictionary-Picard)). This file can be saved and reused.\n14. Lifting over VCFs ([picard LiftoverVcf](https://gatk.broadinstitute.org/hc/en-us/articles/360037060932-LiftoverVcf-Picard))\n15. Lifting over high confidence coordinates ([UCSC liftover](http://hgdownload.cse.ucsc.edu/admin/exe))\n\n### Statistical inference of input test and truth variants:\n\nThis step provides insights into the distribution of variants before benchmarking.\n\n16. Get statistics of SNVs, INDELs and complex variants ([bcftools stats](https://samtools.github.io/bcftools/bcftools.html#stats))\n17. Get statistics of SVs by type ([SURVIVOR stats](https://github.com/fritzsedlazeck/SURVIVOR/wiki))\n\n### Benchmarking of variants:\n\nActual benchmarking of variants are split between SVs and small variants:\n\nAvailable methods for SVs:\n\n18. Germline and somatic variant benchmarking using Truvari ([truvari bench](https://github.com/acenglish/truvari/wiki/bench))\n19. Germline and somatic variant benchmarking using SVanalyzer ([svanalyzer benchmark](https://github.com/nhansen/SVanalyzer/blob/master/docs/svbenchmark.rst))\n\nAvailable methods for CNVs:\n\n20. Germline and somatic variant benchmarking using Wittyer ([witty.er](https://github.com/Illumina/witty.er/tree/master))\n\nAvailable methods for SNVs and INDELs:\n\n21. Germline variant benchmarking using RTG-tools ([rtg vcfeval](https://realtimegenomics.com/products/rtg-tools))\n22. Germline variant benchmarking using Happy tools ([hap.py](https://github.com/Illumina/hap.py/blob/master/doc/happy.md))\n23. Somatic variant benchmarking using Sompy ([som.py](https://github.com/Illumina/hap.py/tree/master?tab=readme-ov-file#sompy))\n\n### Comparison of benchmarking results per TP, FP and FN files\n\nIt is essential to compare benchmarking results in order to infer uniquely or commonly seen TPs, FPs and FNs.\n\n24. Merging TP, FP and FN results for happy, rtgtools and sompy ([bcftools merge](https://samtools.github.io/bcftools/bcftools.html#merge))\n25. Merging TP, FP and FN results for Truvari and SVanalyzer ([SURVIVOR merge](https://github.com/fritzsedlazeck/SURVIVOR/wiki))\n26. Conversion of VCF files to CSV to infer common and unique variants per caller (python script)\n\n### Reporting of benchmark results\n\nThe generation of comprehensive report that consolidates all benchmarking results.\n\n27. Merging summary statistics per benchmarking tool (python script)\n28. Plotting benchmark metrics per benchmarking tool (R script)\n29. Create visual HTML report for the integration of NCBENCH ([datavzrd](https://datavzrd.github.io/docs/index.html))\n30. Apply MultiQC to visualize results\n\n\n1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))\n\n## Usage\n\n> [!NOTE]\n> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) with `-profile test` before running the workflow on actual data.\n\nFirst, prepare a samplesheet with your input data that looks as follows:\n\n`samplesheet.csv`:\n\n```csv\nid,test_vcf,caller\ntest1,test1.vcf.gz,delly\ntest2,test2.vcf,gatk\ntest3,test3.vcf.gz,cnvkit\n```\n\nEach row represents a vcf file (test-query file). For each vcf file and variant calling method (caller) have to be defined.\n\nUser _has to provide truth vcf in config files_. There are readily available vcf files for benchmarking from Genome in a bottle and SEQC2 studies which can be used readily. Please find detailed information about truth files [here](https://nf-co.re/variantbenchmarking/truth)\n\nFor more details and further functionality, please refer to the [usage documentation](https://nf-co.re/variantbenchmarking/usage) and the [parameter documentation](https://nf-co.re/variantbenchmarking/parameters).\n\nNow, you can run the pipeline using:\n\n```bash\nnextflow run nf-core/variantbenchmarking \\\n -profile \\\n --input samplesheet.csv \\\n --outdir \\\n --genome GRCh37 \\\n --analysis germline \\\n --truth_id HG002 \\\n --truth_vcf truth.vcf.gz\n```\n\n> [!WARNING]\n> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files).\n\n## Pipeline output\n\nTo see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/variantbenchmarking/results) tab on the nf-core website pipeline page.\nFor more details about the output files and reports, please refer to the\n[output documentation](https://nf-co.re/variantbenchmarking/output).\n\nThis pipeline outputs benchmarking results per method besides to the inferred and compared statistics.\n\n## Credits\n\nnf-core/variantbenchmarking was originally written by K\u00fcbra Narc\u0131 ([@kubranarci](https://github.com/kubranarci)) as a part of benchmarking studies in German Human Genome Phenome Archieve Project ([GHGA](https://www.ghga.de/)).\n\nWe thank the following people for their extensive assistance in the development of this pipeline:\n\n- Nicolas Vannieuwkerke ([@nvnienwk](https://github.com/nvnieuwk)),\n- Maxime Garcia ([@maxulysse](https://github.com/maxulysse))\n\n## Acknowledgements\n\n\n \"GHGA\"\n\n\n## Contributions and Support\n\nIf you would like to contribute to this pipeline, please see the [contributing guidelines](.github/CONTRIBUTING.md).\n\nFor further information or help, don't hesitate to get in touch on the [Slack `#variantbenchmarking` channel](https://nfcore.slack.com/channels/variantbenchmarking) (you can join with [this invite](https://nf-co.re/join/slack)).\n\n## Citations\n\n\n\n\n\n\nAn extensive list of references for the tools used by the pipeline can be found in the [`CITATIONS.md`](CITATIONS.md) file.\n\nYou can cite the `nf-core` publication as follows:\n\n> **The nf-core framework for community-curated bioinformatics pipelines.**\n>\n> Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen.\n>\n> _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x).\n", "hasPart": [ { "@id": "main.nf" @@ -31,6 +31,9 @@ { "@id": "assets/" }, + { + "@id": "bin/" + }, { "@id": "conf/" }, @@ -43,6 +46,9 @@ { "@id": "modules/" }, + { + "@id": "modules/local/" + }, { "@id": "modules/nf-core/" }, @@ -99,7 +105,7 @@ }, "mentions": [ { - "@id": "#ae45bd39-5f9e-4b26-b5c0-9ca6bc1fa200" + "@id": "#9640bd63-43a6-4d0c-ad47-49c574036111" } ], "name": "nf-core/variantbenchmarking" @@ -121,21 +127,52 @@ }, { "@id": "main.nf", - "@type": ["File", "SoftwareSourceCode", "ComputationalWorkflow"], + "@type": [ + "File", + "SoftwareSourceCode", + "ComputationalWorkflow" + ], + "creator": [ + { + "@id": "https://orcid.org/0000-0002-3532-2152" + }, + { + "@id": "https://orcid.org/0009-0003-5619-1555" + }, + { + "@id": "#max.u.garcia@gmail.com" + } + ], "dateCreated": "", - "dateModified": "2025-01-27T14:49:17Z", + "dateModified": "2025-02-03T12:56:47Z", "dct:conformsTo": "https://bioschemas.org/profiles/ComputationalWorkflow/1.0-RELEASE/", - "keywords": ["nf-core", "nextflow", "benchmark", "draft", "structural-variants", "variant-calling"], - "license": ["MIT"], - "name": ["nf-core/variantbenchmarking"], + "keywords": [ + "nf-core", + "nextflow", + "benchmark", + "draft", + "structural-variants", + "variant-calling" + ], + "license": [ + "MIT" + ], + "name": [ + "nf-core/variantbenchmarking" + ], "programmingLanguage": { "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow" }, "sdPublisher": { "@id": "https://nf-co.re/" }, - "url": ["https://github.com/nf-core/variantbenchmarking", "https://nf-co.re/variantbenchmarking/dev/"], - "version": ["1.0dev"] + "url": [ + "https://github.com/nf-core/variantbenchmarking", + "https://nf-co.re/variantbenchmarking/1.0.0/" + ], + "version": [ + "1.0.0" + ] }, { "@id": "https://w3id.org/workflowhub/workflow-ro-crate#nextflow", @@ -150,11 +187,11 @@ "version": "!>=24.04.2" }, { - "@id": "#ae45bd39-5f9e-4b26-b5c0-9ca6bc1fa200", + "@id": "#9640bd63-43a6-4d0c-ad47-49c574036111", "@type": "TestSuite", "instance": [ { - "@id": "#5b391f3e-435e-4c5f-8beb-cb6dc0854d4f" + "@id": "#1cc1a4dd-2f43-4274-baf5-d97249264afb" } ], "mainEntity": { @@ -163,7 +200,7 @@ "name": "Test suite for nf-core/variantbenchmarking" }, { - "@id": "#5b391f3e-435e-4c5f-8beb-cb6dc0854d4f", + "@id": "#1cc1a4dd-2f43-4274-baf5-d97249264afb", "@type": "TestInstance", "name": "GitHub Actions workflow for testing nf-core/variantbenchmarking", "resource": "repos/nf-core/variantbenchmarking/actions/workflows/ci.yml", @@ -185,6 +222,11 @@ "@type": "Dataset", "description": "Additional files" }, + { + "@id": "bin/", + "@type": "Dataset", + "description": "Scripts that must be callable from a pipeline process" + }, { "@id": "conf/", "@type": "Dataset", @@ -205,6 +247,11 @@ "@type": "Dataset", "description": "Modules used by the pipeline" }, + { + "@id": "modules/local/", + "@type": "Dataset", + "description": "Pipeline-specific modules" + }, { "@id": "modules/nf-core/", "@type": "Dataset", @@ -290,6 +337,24 @@ "@type": "Organization", "name": "nf-core", "url": "https://nf-co.re/" + }, + { + "@id": "https://orcid.org/0000-0002-3532-2152", + "@type": "Person", + "email": "kbrnrc@gmail.com", + "name": "K\u00fcbra Narc\u0131" + }, + { + "@id": "https://orcid.org/0009-0003-5619-1555", + "@type": "Person", + "email": "101190534+nvnieuwk@users.noreply.github.com", + "name": "Nicolas Vannieuwkerke" + }, + { + "@id": "#max.u.garcia@gmail.com", + "@type": "Person", + "email": "max.u.garcia@gmail.com", + "name": "Maxime U Garcia" } ] -} +} \ No newline at end of file From 92e43fad276cdceb5df1b88156dd74e8426fcbaf Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 3 Feb 2025 13:07:57 +0000 Subject: [PATCH 279/292] correct email --- nextflow.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 4a8045c..404371a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -280,7 +280,7 @@ manifest { contributors = [ // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0 [ - name: 'kuebra.narci@dkfz.de', + name: 'kuebra.narci@dkfz-heidelberg.de', affiliation: '', email: '', github: '', From 1e0d0eeb6a6c19f67854a79004e54205b70aae53 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 4 Feb 2025 11:43:56 +0000 Subject: [PATCH 280/292] need to change test files --- conf/tests/germline_sv.config | 14 ++-- conf/tests/somatic_sv.config | 2 +- nextflow.config | 15 ++-- tests/germline_sv.nf.test | 2 + tests/germline_sv.nf.test.snap | 116 ++++++++++++++-------------- tests/somatic_sv.nf.test | 1 + tests/somatic_sv.nf.test.snap | 133 +++++++++++++++++---------------- 7 files changed, 145 insertions(+), 138 deletions(-) diff --git a/conf/tests/germline_sv.config b/conf/tests/germline_sv.config index 7af553a..44aa09b 100644 --- a/conf/tests/germline_sv.config +++ b/conf/tests/germline_sv.config @@ -23,23 +23,23 @@ params { config_profile_description = 'Minimal test dataset to check pipeline function' // Input data - input = "${params.test_data_base}/samplesheet/samplesheet_sv_germline_hg38.csv" + input = "${params.test_data_base}/samplesheet/samplesheet_sv_germline_hg37.csv" outdir = "results" // Genome references - genome = 'GRCh38' + genome = 'GRCh37' // Processes analysis = 'germline' variant_type = "structural" method = 'svanalyzer,wittyer,truvari' - preprocess = "normalize,deduplicate,filter_contigs" - sv_standardization = "svync,homogenize" + preprocess = "split_multiallelic,normalize,deduplicate,filter_contigs" + sv_standardization = "svync" min_sv_size = 30 truth_id = "HG002" include_expression = 'FILTER="."' - truth_vcf = "${params.test_data_base}/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v0.01.chr21.vcf.gz" - regions_bed = "${params.test_data_base}/testdata/hg38/truth/germline/HG002_GRCh38_difficult_medical_gene_SV_benchmark_v01.ch21.bed" - rename_chr = "${projectDir}/assets/rename_contigs/grch37_grch38.txt" + truth_vcf = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.vcf.gz" + regions_bed = "${params.test_data_base}/testdata/hg37/truth/germline/HG002_SVs_Tier1_v0.6.chr21.bed" + rename_chr = "${projectDir}/assets/rename_contigs/grch38_grch37.txt" } diff --git a/conf/tests/somatic_sv.config b/conf/tests/somatic_sv.config index d3cbde3..4df2908 100644 --- a/conf/tests/somatic_sv.config +++ b/conf/tests/somatic_sv.config @@ -27,7 +27,7 @@ params { genome = "GRCh38" analysis = "somatic" variant_type = "structural" - method = "truvari" + method = "svanalyzer" preprocess = "filter_contigs" truth_id = "SEQC2" diff --git a/nextflow.config b/nextflow.config index 404371a..ca1e0b0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -276,17 +276,16 @@ prov { manifest { name = 'nf-core/variantbenchmarking' - author = """kuebra.narci@dkfz.de""" // The author field is deprecated from Nextflow version 24.10.0, use contributors instead + author = """kuebra.narci@dkfz-heidelberg.de""" // The author field is deprecated from Nextflow version 24.10.0, use contributors instead contributors = [ - // TODO nf-core: Update the field with the details of the contributors to your pipeline. New with Nextflow version 24.10.0 [ - name: 'kuebra.narci@dkfz-heidelberg.de', - affiliation: '', - email: '', - github: '', + name: 'Kuebra Narci', + affiliation: 'GHGA', + email: 'kuebra.narci@dkfz-heidelberg.de', + github: '@kubranarci', contribution: ['author', 'maintainer'], // List of contribution types ('author', 'maintainer' or 'contributor') - orcid: '' - ], + orcid: '0000-0002-3532-2152' + ] ] homePage = 'https://github.com/nf-core/variantbenchmarking' description = """Variant Benchmarking pipeline for germline and somatic variant callers """ diff --git a/tests/germline_sv.nf.test b/tests/germline_sv.nf.test index 1ace554..4bbb791 100644 --- a/tests/germline_sv.nf.test +++ b/tests/germline_sv.nf.test @@ -12,6 +12,7 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' } } @@ -42,6 +43,7 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' } } diff --git a/tests/germline_sv.nf.test.snap b/tests/germline_sv.nf.test.snap index b854110..c0a428b 100644 --- a/tests/germline_sv.nf.test.snap +++ b/tests/germline_sv.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'germline' --variant_type 'structural' --method 'truvari,svbenchmark,wittyer'": { "content": [ - 121, + 119, null, [ "pipeline_info", @@ -198,33 +198,33 @@ "structural/test2", "structural/test2/benchmarks", "structural/test2/benchmarks/svanalyzer", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.distances", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.falsenegatives.vcf.gz", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.falsepositives.vcf.gz", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.log", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.report", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.distances", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.falsenegatives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.falsepositives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.log", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.report", "structural/test2/benchmarks/truvari", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fn.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fn.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fp.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.merged.summary.json", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-base.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-base.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fn.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fn.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fp.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fp.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.summary.json", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-base.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-base.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-comp.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-comp.vcf.gz.tbi", "structural/test2/benchmarks/wittyer", - "structural/test2/benchmarks/wittyer/test2.HG002.merged.json", - "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz", - "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz.tbi", + "structural/test2/benchmarks/wittyer/test2.HG002.lumpy.json", + "structural/test2/benchmarks/wittyer/test2.HG002.lumpy.vcf.gz", + "structural/test2/benchmarks/wittyer/test2.HG002.lumpy.vcf.gz.tbi", "structural/test2/preprocess", "structural/test2/preprocess/test2.vcf.gz", "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", "structural/test2/stats/bcftools", - "structural/test2/stats/bcftools/test2.merged.bcftools_stats.txt", + "structural/test2/stats/bcftools/test2.lumpy.bcftools_stats.txt", "structural/test2/stats/survivor", - "structural/test2/stats/survivor/test2.merged_mqc.stats", + "structural/test2/stats/survivor/test2.lumpy_mqc.stats", "structural/test3", "structural/test3/benchmarks", "structural/test3/benchmarks/svanalyzer", @@ -257,19 +257,19 @@ "structural/test3/stats/survivor/test3.dragen_mqc.stats" ], [ - "HG002.bcftools_stats.txt:md5,06ee97dcd1b34ddca03a7e5f9af23e68", - "HG002_mqc.stats:md5,68681df47b35e3193be03610f5c6e3d6", - "test1.HG002.manta.distances:md5,346f18a5cbeece98716951c8fc2aaea4", - "test1.HG002.manta.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", - "test1.manta.bcftools_stats.txt:md5,9a82df362fe77db3330544f2b98b439e", - "test1.manta_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", - "test2.HG002.merged.distances:md5,346f18a5cbeece98716951c8fc2aaea4", - "test2.HG002.merged.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", - "test2.merged.bcftools_stats.txt:md5,bbd579cedaf78b6199fb71e52acfc14d", - "test2.merged_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b", + "HG002.bcftools_stats.txt:md5,bc95472c545c41b97fc6c657ebb1132b", + "HG002_mqc.stats:md5,ba6a6398a99b95579872576cacde4c8c", + "test1.HG002.manta.distances:md5,b66127be418228969d3db50dd54f82ae", + "test1.HG002.manta.report:md5,710cf6d3a7d94b82ce40fd6fab195f35", + "test1.manta.bcftools_stats.txt:md5,db893fb61ca1ec2f2be4dcf17502c78f", + "test1.manta_mqc.stats:md5,377494563323ef6d078e71ded42c24ce", + "test2.HG002.lumpy.distances:md5,0f8f3a33cfaa22913b1e76596c6886f1", + "test2.HG002.lumpy.report:md5,ce5bd09b229e0cdf0001766cdeb0f854", + "test2.lumpy.bcftools_stats.txt:md5,681916c18ba7f61815fec0b09e390922", + "test2.lumpy_mqc.stats:md5,1c029d583c409ea1e32658dd1e5c4e4a", "test3.HG002.dragen.distances:md5,346f18a5cbeece98716951c8fc2aaea4", - "test3.HG002.dragen.report:md5,4a53712a9d15fa6dfe6ddd5848ca691c", - "test3.dragen.bcftools_stats.txt:md5,73648a9eb3b6cd5049f579ef861637b2", + "test3.HG002.dragen.report:md5,3e6ae1e90331b23af9c37679d84114b9", + "test3.dragen.bcftools_stats.txt:md5,1b51ef9562e3ee97953c1f6714b5b917", "test3.dragen_mqc.stats:md5,011ad66fec4287d32cb728c40e240c0b" ] ], @@ -277,11 +277,11 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-29T12:17:47.644313415" + "timestamp": "2025-02-04T10:44:22.883993767" }, "-stub": { "content": [ - 121, + 119, null, [ "pipeline_info", @@ -417,33 +417,33 @@ "structural/test2", "structural/test2/benchmarks", "structural/test2/benchmarks/svanalyzer", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.distances", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.falsenegatives.vcf.gz", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.falsepositives.vcf.gz", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.log", - "structural/test2/benchmarks/svanalyzer/test2.HG002.merged.report", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.distances", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.falsenegatives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.falsepositives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.log", + "structural/test2/benchmarks/svanalyzer/test2.HG002.lumpy.report", "structural/test2/benchmarks/truvari", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fn.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fn.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.fp.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.merged.summary.json", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-base.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-base.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.HG002.merged.tp-comp.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fn.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fn.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fp.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.fp.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.summary.json", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-base.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-base.vcf.gz.tbi", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-comp.vcf.gz", + "structural/test2/benchmarks/truvari/test2.HG002.lumpy.tp-comp.vcf.gz.tbi", "structural/test2/benchmarks/wittyer", - "structural/test2/benchmarks/wittyer/test2.HG002.merged.json", - "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz", - "structural/test2/benchmarks/wittyer/test2.HG002.merged.vcf.gz.tbi", + "structural/test2/benchmarks/wittyer/test2.HG002.lumpy.json", + "structural/test2/benchmarks/wittyer/test2.HG002.lumpy.vcf.gz", + "structural/test2/benchmarks/wittyer/test2.HG002.lumpy.vcf.gz.tbi", "structural/test2/preprocess", "structural/test2/preprocess/test2.vcf.gz", "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", "structural/test2/stats/bcftools", - "structural/test2/stats/bcftools/test2.merged.bcftools_stats.txt", + "structural/test2/stats/bcftools/test2.lumpy.bcftools_stats.txt", "structural/test2/stats/survivor", - "structural/test2/stats/survivor/test2.merged_mqc.stats", + "structural/test2/stats/survivor/test2.lumpy_mqc.stats", "structural/test3", "structural/test3/benchmarks", "structural/test3/benchmarks/svanalyzer", @@ -482,10 +482,10 @@ "test1.HG002.manta.report:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.manta.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", "test1.manta_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.merged.distances:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.HG002.merged.report:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.merged.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", - "test2.merged_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.HG002.lumpy.distances:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.HG002.lumpy.report:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.lumpy.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", + "test2.lumpy_mqc.stats:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.HG002.dragen.distances:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.HG002.dragen.report:md5,d41d8cd98f00b204e9800998ecf8427e", "test3.dragen.bcftools_stats.txt:md5,d41d8cd98f00b204e9800998ecf8427e", @@ -496,6 +496,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-29T12:20:02.006578062" + "timestamp": "2025-02-04T11:12:23.705004318" } } \ No newline at end of file diff --git a/tests/somatic_sv.nf.test b/tests/somatic_sv.nf.test index 3d2cc3b..1c510cd 100644 --- a/tests/somatic_sv.nf.test +++ b/tests/somatic_sv.nf.test @@ -12,6 +12,7 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' } } diff --git a/tests/somatic_sv.nf.test.snap b/tests/somatic_sv.nf.test.snap index ffd0919..17b4094 100644 --- a/tests/somatic_sv.nf.test.snap +++ b/tests/somatic_sv.nf.test.snap @@ -1,7 +1,7 @@ { "Params: --analysis 'somatic' --variant_type 'structural' --method 'truvari,svbenchmark'": { "content": [ - 53, + 37, null, [ "pipeline_info", @@ -18,7 +18,9 @@ "structural/SEQC2/stats/survivor/SEQC2_mqc.stats", "structural/multiqc", "structural/multiqc/multiqc_data", + "structural/multiqc/multiqc_data/bcftools-stats-subtypes.txt", "structural/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "structural/multiqc/multiqc_data/bcftools_stats_variant_depths.txt", "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", "structural/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", @@ -33,7 +35,10 @@ "structural/multiqc/multiqc_data/multiqc_survivor.txt", "structural/multiqc/multiqc_plots", "structural/multiqc/multiqc_plots/pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "structural/multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", "structural/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", @@ -42,7 +47,10 @@ "structural/multiqc/multiqc_plots/pdf/survivor-cnt.pdf", "structural/multiqc/multiqc_plots/pdf/survivor-pct.pdf", "structural/multiqc/multiqc_plots/png", + "structural/multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png", + "structural/multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "structural/multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", "structural/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", @@ -51,7 +59,10 @@ "structural/multiqc/multiqc_plots/png/survivor-cnt.png", "structural/multiqc/multiqc_plots/png/survivor-pct.png", "structural/multiqc/multiqc_plots/svg", + "structural/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg", + "structural/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "structural/multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", "structural/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", @@ -62,98 +73,92 @@ "structural/multiqc/multiqc_report.html", "structural/summary", "structural/summary/comparisons", - "structural/summary/comparisons/truvari.FN.csv", - "structural/summary/comparisons/truvari.FP.csv", - "structural/summary/comparisons/truvari.TP_base.csv", - "structural/summary/comparisons/truvari.TP_comp.csv", + "structural/summary/comparisons/svbenchmark.FN.csv", + "structural/summary/comparisons/svbenchmark.FP.csv", "structural/summary/datavzrd", - "structural/summary/datavzrd/truvari", - "structural/summary/datavzrd/truvari/index.html", - "structural/summary/datavzrd/truvari/static", - "structural/summary/datavzrd/truvari/static/bootstrap-select.min.css", - "structural/summary/datavzrd/truvari/static/bootstrap-table-fixed-columns.min.css", - "structural/summary/datavzrd/truvari/static/bootstrap-table.min.css", - "structural/summary/datavzrd/truvari/static/bootstrap.min.css", - "structural/summary/datavzrd/truvari/static/bundle.js", - "structural/summary/datavzrd/truvari/static/datavzrd.css", - "structural/summary/datavzrd/truvari/test", - 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"structural/summary/datavzrd/svbenchmark/static", + "structural/summary/datavzrd/svbenchmark/static/bootstrap-select.min.css", + "structural/summary/datavzrd/svbenchmark/static/bootstrap-table-fixed-columns.min.css", + "structural/summary/datavzrd/svbenchmark/static/bootstrap-table.min.css", + "structural/summary/datavzrd/svbenchmark/static/bootstrap.min.css", + "structural/summary/datavzrd/svbenchmark/static/bundle.js", + "structural/summary/datavzrd/svbenchmark/static/datavzrd.css", + "structural/summary/datavzrd/svbenchmark/test", + "structural/summary/datavzrd/svbenchmark/test/config.js", + "structural/summary/datavzrd/svbenchmark/test/data", + "structural/summary/datavzrd/svbenchmark/test/data/data_1.js", + "structural/summary/datavzrd/svbenchmark/test/functions.js", + "structural/summary/datavzrd/svbenchmark/test/heatmap.js", + "structural/summary/datavzrd/svbenchmark/test/index_1.html", + "structural/summary/datavzrd/svbenchmark/test/plots", + 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"structural/test1/benchmarks/svanalyzer", + "structural/test1/benchmarks/svanalyzer/test1.SEQC2.freebayes.distances", + "structural/test1/benchmarks/svanalyzer/test1.SEQC2.freebayes.falsenegatives.vcf.gz", + "structural/test1/benchmarks/svanalyzer/test1.SEQC2.freebayes.falsepositives.vcf.gz", + "structural/test1/benchmarks/svanalyzer/test1.SEQC2.freebayes.log", + "structural/test1/benchmarks/svanalyzer/test1.SEQC2.freebayes.report", "structural/test1/preprocess", "structural/test1/preprocess/test1.vcf.gz", "structural/test1/preprocess/test1.vcf.gz.tbi", "structural/test1/stats", "structural/test1/stats/bcftools", - "structural/test1/stats/bcftools/test1.tiddit.bcftools_stats.txt", + "structural/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", "structural/test1/stats/survivor", - "structural/test1/stats/survivor/test1.tiddit_mqc.stats", + "structural/test1/stats/survivor/test1.freebayes_mqc.stats", "structural/test2", "structural/test2/benchmarks", - "structural/test2/benchmarks/truvari", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.fn.vcf.gz", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.fn.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.fp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.fp.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.summary.json", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.tp-base.vcf.gz", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.tp-base.vcf.gz.tbi", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.tp-comp.vcf.gz", - "structural/test2/benchmarks/truvari/test2.SEQC2.manta.tp-comp.vcf.gz.tbi", + "structural/test2/benchmarks/svanalyzer", + "structural/test2/benchmarks/svanalyzer/test2.SEQC2.strelka.distances", + "structural/test2/benchmarks/svanalyzer/test2.SEQC2.strelka.falsenegatives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.SEQC2.strelka.falsepositives.vcf.gz", + "structural/test2/benchmarks/svanalyzer/test2.SEQC2.strelka.log", + "structural/test2/benchmarks/svanalyzer/test2.SEQC2.strelka.report", "structural/test2/preprocess", "structural/test2/preprocess/test2.vcf.gz", "structural/test2/preprocess/test2.vcf.gz.tbi", "structural/test2/stats", "structural/test2/stats/bcftools", - "structural/test2/stats/bcftools/test2.manta.bcftools_stats.txt", + "structural/test2/stats/bcftools/test2.strelka.bcftools_stats.txt", "structural/test2/stats/survivor", - "structural/test2/stats/survivor/test2.manta_mqc.stats" + "structural/test2/stats/survivor/test2.strelka_mqc.stats" ], [ "SEQC2.bcftools_stats.txt:md5,e1a7c33b75d3e2688ba48b1b9975b467", "SEQC2_mqc.stats:md5,cb253f3829a25286d572c91a9ea75e9e", - "test1.tiddit.bcftools_stats.txt:md5,cbd62a9be5344b2d6223f8600483e6a2", - "test1.tiddit_mqc.stats:md5,0eb749429a6072f6bf63d377e37514d1", - "test2.manta.bcftools_stats.txt:md5,1926259ac4ad828a4a50a8e07e2b1d6f", - "test2.manta_mqc.stats:md5,73bc60cd2754202ddca8be6552e85ffa" + "test1.SEQC2.freebayes.distances:md5,346f18a5cbeece98716951c8fc2aaea4", + "test1.SEQC2.freebayes.report:md5,8140e61dd8527ba0cbd63cb52aa93219", + "test1.freebayes.bcftools_stats.txt:md5,4a7f705c0411cf01c3ec0914b945dcfd", + "test1.freebayes_mqc.stats:md5,d7782c48b7721cc5a707ed913f7df910", + "test2.SEQC2.strelka.distances:md5,346f18a5cbeece98716951c8fc2aaea4", + "test2.SEQC2.strelka.report:md5,151562898a99713affbcb3e834d824ad", + "test2.strelka.bcftools_stats.txt:md5,1d41ebd0fd21a2a3d204b4ce390db815", + "test2.strelka_mqc.stats:md5,ec044ea659043ecbc5a140d040d871c6" ] ], "meta": { "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-30T09:50:51.993793161" + "timestamp": "2025-02-04T10:56:57.458901208" } } \ No newline at end of file From f9ae0677f89c125ae946abf35d40999637ac3b8c Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 4 Feb 2025 13:28:59 +0000 Subject: [PATCH 281/292] add test_data_base to tests --- tests/germline_small.nf.test | 4 + tests/liftover_test.nf.test | 2 + tests/liftover_truth.nf.test | 2 + tests/somatic_indel.nf.test | 2 + tests/somatic_snv.nf.test | 20 ++++- tests/somatic_snv.nf.test.snap | 160 +++++++++++++++++++++++++++++++++ 6 files changed, 189 insertions(+), 1 deletion(-) diff --git a/tests/germline_small.nf.test b/tests/germline_small.nf.test index ace53fa..250356f 100644 --- a/tests/germline_small.nf.test +++ b/tests/germline_small.nf.test @@ -12,6 +12,8 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } } @@ -42,6 +44,8 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } } diff --git a/tests/liftover_test.nf.test b/tests/liftover_test.nf.test index b049a2b..231241a 100644 --- a/tests/liftover_test.nf.test +++ b/tests/liftover_test.nf.test @@ -14,6 +14,8 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } } diff --git a/tests/liftover_truth.nf.test b/tests/liftover_truth.nf.test index f3e94ce..d421272 100644 --- a/tests/liftover_truth.nf.test +++ b/tests/liftover_truth.nf.test @@ -14,6 +14,8 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } } diff --git a/tests/somatic_indel.nf.test b/tests/somatic_indel.nf.test index 9901eac..6e109b0 100644 --- a/tests/somatic_indel.nf.test +++ b/tests/somatic_indel.nf.test @@ -12,6 +12,8 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } } diff --git a/tests/somatic_snv.nf.test b/tests/somatic_snv.nf.test index 4262d34..553e685 100644 --- a/tests/somatic_snv.nf.test +++ b/tests/somatic_snv.nf.test @@ -12,12 +12,28 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } } then { + // stable_name: All files + folders in ${params.outdir}/ with a stable name + def stable_name = getAllFilesFromDir(params.outdir, relative: true, includeDir: true, ignore: ['pipeline_info/*.{html,json,txt}']) + // stable_path: All files in ${params.outdir}/ with stable content + def stable_path = getAllFilesFromDir(params.outdir, ignoreFile: 'tests/.nftignore') assertAll( - { assert workflow.success } + { assert workflow.success }, + { assert snapshot( + // Number of successful tasks + workflow.trace.succeeded().size(), + // pipeline versions.yml file for multiqc from which Nextflow version is removed because we tests pipelines on multiple Nextflow versions + removeNextflowVersion("$outputDir/pipeline_info/nf_core_pipeline_software_mqc_versions.yml"), + // All stable path name, with a relative path + stable_name, + // All files with stable contents + stable_path + ).match() } ) } } @@ -28,6 +44,8 @@ nextflow_pipeline { when { params { outdir = "$outputDir" + test_data_base = 'https://raw.githubusercontent.com/nf-core/test-datasets/variantbenchmarking' + } } diff --git a/tests/somatic_snv.nf.test.snap b/tests/somatic_snv.nf.test.snap index 907ddc0..71b882a 100644 --- a/tests/somatic_snv.nf.test.snap +++ b/tests/somatic_snv.nf.test.snap @@ -96,5 +96,165 @@ "nextflow": "24.10.4" }, "timestamp": "2025-01-29T17:10:57.898182832" + }, + "Params: --analysis 'somatic' --variant_type 'snv' --method 'sompy'": { + "content": [ + 29, + null, + [ + "pipeline_info", + "pipeline_info/nf_core_variantbenchmarking_software_mqc_versions.yml", + "snv", + "snv/SEQC2", + "snv/SEQC2/preprocess", + "snv/SEQC2/preprocess/SEQC2.vcf.gz", + "snv/SEQC2/preprocess/SEQC2.vcf.gz.tbi", + "snv/SEQC2/stats", + "snv/SEQC2/stats/bcftools", + "snv/SEQC2/stats/bcftools/SEQC2.bcftools_stats.txt", + "snv/multiqc", + "snv/multiqc/multiqc_data", + "snv/multiqc/multiqc_data/bcftools-stats-subtypes.txt", + "snv/multiqc/multiqc_data/bcftools_stats_indel-lengths.txt", + "snv/multiqc/multiqc_data/bcftools_stats_variant_depths.txt", + "snv/multiqc/multiqc_data/bcftools_stats_vqc_Count_Indels.txt", + "snv/multiqc/multiqc_data/bcftools_stats_vqc_Count_SNP.txt", + "snv/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transitions.txt", + "snv/multiqc/multiqc_data/bcftools_stats_vqc_Count_Transversions.txt", + "snv/multiqc/multiqc_data/multiqc.log", + "snv/multiqc/multiqc_data/multiqc_bcftools_stats.txt", + "snv/multiqc/multiqc_data/multiqc_citations.txt", + "snv/multiqc/multiqc_data/multiqc_data.json", + "snv/multiqc/multiqc_data/multiqc_general_stats.txt", + "snv/multiqc/multiqc_data/multiqc_software_versions.txt", + "snv/multiqc/multiqc_data/multiqc_sources.txt", + "snv/multiqc/multiqc_plots", + "snv/multiqc/multiqc_plots/pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-cnt.pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools-stats-subtypes-pct.pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools_stats_indel-lengths.pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools_stats_variant_depths.pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Indels.pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_SNP.pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transitions.pdf", + "snv/multiqc/multiqc_plots/pdf/bcftools_stats_vqc_Count_Transversions.pdf", + "snv/multiqc/multiqc_plots/pdf/general_stats_table.pdf", + "snv/multiqc/multiqc_plots/png", + "snv/multiqc/multiqc_plots/png/bcftools-stats-subtypes-cnt.png", + "snv/multiqc/multiqc_plots/png/bcftools-stats-subtypes-pct.png", + "snv/multiqc/multiqc_plots/png/bcftools_stats_indel-lengths.png", + "snv/multiqc/multiqc_plots/png/bcftools_stats_variant_depths.png", + "snv/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Indels.png", + "snv/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_SNP.png", + "snv/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transitions.png", + "snv/multiqc/multiqc_plots/png/bcftools_stats_vqc_Count_Transversions.png", + "snv/multiqc/multiqc_plots/png/general_stats_table.png", + "snv/multiqc/multiqc_plots/svg", + "snv/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-cnt.svg", + "snv/multiqc/multiqc_plots/svg/bcftools-stats-subtypes-pct.svg", + "snv/multiqc/multiqc_plots/svg/bcftools_stats_indel-lengths.svg", + "snv/multiqc/multiqc_plots/svg/bcftools_stats_variant_depths.svg", + "snv/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Indels.svg", + "snv/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_SNP.svg", + "snv/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transitions.svg", + "snv/multiqc/multiqc_plots/svg/bcftools_stats_vqc_Count_Transversions.svg", + "snv/multiqc/multiqc_plots/svg/general_stats_table.svg", + "snv/multiqc/multiqc_report.html", + "snv/summary", + "snv/summary/datavzrd", + "snv/summary/datavzrd/sompy", + "snv/summary/datavzrd/sompy/index.html", + "snv/summary/datavzrd/sompy/static", + "snv/summary/datavzrd/sompy/static/bootstrap-select.min.css", + "snv/summary/datavzrd/sompy/static/bootstrap-table-fixed-columns.min.css", + "snv/summary/datavzrd/sompy/static/bootstrap-table.min.css", + "snv/summary/datavzrd/sompy/static/bootstrap.min.css", + "snv/summary/datavzrd/sompy/static/bundle.js", + "snv/summary/datavzrd/sompy/static/datavzrd.css", + "snv/summary/datavzrd/sompy/test", + "snv/summary/datavzrd/sompy/test/config.js", + "snv/summary/datavzrd/sompy/test/data", + "snv/summary/datavzrd/sompy/test/data/data_1.js", + "snv/summary/datavzrd/sompy/test/functions.js", + "snv/summary/datavzrd/sompy/test/heatmap.js", + "snv/summary/datavzrd/sompy/test/index_1.html", + "snv/summary/datavzrd/sompy/test/plots", + "snv/summary/datavzrd/sompy/test/plots/plot_0.js", + "snv/summary/datavzrd/sompy/test/plots/plot_1.js", + "snv/summary/datavzrd/sompy/test/plots/plot_10.js", + "snv/summary/datavzrd/sompy/test/plots/plot_11.js", + "snv/summary/datavzrd/sompy/test/plots/plot_12.js", + "snv/summary/datavzrd/sompy/test/plots/plot_13.js", + "snv/summary/datavzrd/sompy/test/plots/plot_14.js", + "snv/summary/datavzrd/sompy/test/plots/plot_15.js", + "snv/summary/datavzrd/sompy/test/plots/plot_16.js", + "snv/summary/datavzrd/sompy/test/plots/plot_17.js", + "snv/summary/datavzrd/sompy/test/plots/plot_18.js", + "snv/summary/datavzrd/sompy/test/plots/plot_2.js", + "snv/summary/datavzrd/sompy/test/plots/plot_3.js", + "snv/summary/datavzrd/sompy/test/plots/plot_4.js", + "snv/summary/datavzrd/sompy/test/plots/plot_5.js", + "snv/summary/datavzrd/sompy/test/plots/plot_6.js", + "snv/summary/datavzrd/sompy/test/plots/plot_7.js", + "snv/summary/datavzrd/sompy/test/plots/plot_8.js", + "snv/summary/datavzrd/sompy/test/plots/plot_9.js", + "snv/summary/datavzrd/versions.yml", + "snv/summary/plots", + "snv/summary/plots/sompy", + "snv/summary/plots/sompy/metric_by_tool_sompy_mqc.png", + "snv/summary/plots/sompy/variants_by_tool_sompy_mqc.png", + "snv/summary/tables", + "snv/summary/tables/sompy", + "snv/summary/tables/sompy/sompy.regions.csv", + "snv/summary/tables/sompy/sompy.summary.csv", + "snv/test1", + "snv/test1/benchmarks", + "snv/test1/benchmarks/sompy", + "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.features.csv", + "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.metrics.json", + "snv/test1/benchmarks/sompy/test1.SEQC2.freebayes.stats.csv", + "snv/test1/preprocess", + "snv/test1/preprocess/test1.vcf.gz", + "snv/test1/preprocess/test1.vcf.gz.tbi", + "snv/test1/stats", + "snv/test1/stats/bcftools", + "snv/test1/stats/bcftools/test1.freebayes.bcftools_stats.txt", + "snv/test2", + "snv/test2/benchmarks", + "snv/test2/benchmarks/sompy", + "snv/test2/benchmarks/sompy/test2.SEQC2.manta.features.csv", + "snv/test2/benchmarks/sompy/test2.SEQC2.manta.metrics.json", + "snv/test2/benchmarks/sompy/test2.SEQC2.manta.stats.csv", + "snv/test2/preprocess", + "snv/test2/preprocess/test2.vcf.gz", + "snv/test2/preprocess/test2.vcf.gz.tbi", + "snv/test2/stats", + "snv/test2/stats/bcftools", + "snv/test2/stats/bcftools/test2.manta.bcftools_stats.txt", + "snv/test3", + "snv/test3/benchmarks", + "snv/test3/benchmarks/sompy", + "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.features.csv", + "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.metrics.json", + "snv/test3/benchmarks/sompy/test3.SEQC2.strelka.stats.csv", + "snv/test3/preprocess", + "snv/test3/preprocess/test3.vcf.gz", + "snv/test3/preprocess/test3.vcf.gz.tbi", + "snv/test3/stats", + "snv/test3/stats/bcftools", + "snv/test3/stats/bcftools/test3.strelka.bcftools_stats.txt" + ], + [ + "SEQC2.bcftools_stats.txt:md5,2a23dd61a56e7e6435a8ba4617fd793d", + "test1.freebayes.bcftools_stats.txt:md5,ca4f9a72e5cb852b78dbdf92142a4a6f", + "test2.manta.bcftools_stats.txt:md5,0806ab1c22d599b40abc4d22a129a1e2", + "test3.strelka.bcftools_stats.txt:md5,9fc6a285b83b2c2652080562ba9e94d6" + ] + ], + "meta": { + "nf-test": "0.9.2", + "nextflow": "24.10.4" + }, + "timestamp": "2025-02-04T13:25:45.366644429" } } \ No newline at end of file From c9eca8981726d2b6533d0f29abbf5529a6e18379 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Tue, 4 Feb 2025 14:04:32 +0000 Subject: [PATCH 282/292] remove conda from checks --- .github/workflows/ci.yml | 7 ++++--- README.md | 1 + 2 files changed, 5 insertions(+), 3 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index fe5cfe5..4771574 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -1,10 +1,12 @@ name: nf-core CI # This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors on: - pull_request: + push: branches: - dev - - master + pull_request: + paths-ignore: + - "docs/**" release: types: [published] workflow_dispatch: @@ -35,7 +37,6 @@ jobs: - "24.04.2" - "latest-everything" profile: - - "conda" - "docker" - "singularity" isMaster: diff --git a/README.md b/README.md index 4f75533..b41ee1e 100644 --- a/README.md +++ b/README.md @@ -143,6 +143,7 @@ nextflow run nf-core/variantbenchmarking \ > [!WARNING] > Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; see [docs](https://nf-co.re/docs/usage/getting_started/configuration#custom-configuration-files). +> Conda profile is not available for SVanalyzer (SVBenchmark) tool, if you are planing to use the tool either choose docker or singularity. ## Pipeline output From ff15254144917cdb83f0a82570fa4a408e146c48 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Wed, 5 Feb 2025 08:54:23 +0000 Subject: [PATCH 283/292] changes requested by Nicolas --- README.md | 15 +- conf/modules.config | 8 - docs/output.md | 145 +++++++++--------- modules/local/bamsurgeon/evaluator/main.nf | 46 ------ .../create_datavzrd_input/environment.yml | 7 + .../custom/create_datavzrd_input/main.nf | 12 +- .../publish_processed_vcf/environment.yml | 7 +- .../custom/publish_processed_vcf/main.nf | 15 +- modules/local/custom/sort_bed/environment.yml | 7 + modules/local/custom/sort_bed/main.nf | 13 +- nextflow.config | 18 +-- subworkflows/local/liftover_vcfs/main.nf | 12 +- subworkflows/local/prepare_vcfs_truth/main.nf | 10 +- .../local/small_somatic_benchmark/main.nf | 21 --- .../local/sv_somatic_benchmark/main.nf | 40 ----- subworkflows/local/sv_vcf_conversion/main.nf | 2 - tests/somatic_indel.nf.test.snap | 2 +- 17 files changed, 161 insertions(+), 219 deletions(-) delete mode 100644 modules/local/bamsurgeon/evaluator/main.nf create mode 100644 modules/local/custom/create_datavzrd_input/environment.yml create mode 100644 modules/local/custom/sort_bed/environment.yml delete mode 100644 subworkflows/local/sv_somatic_benchmark/main.nf diff --git a/README.md b/README.md index b41ee1e..d19fe99 100644 --- a/README.md +++ b/README.md @@ -75,10 +75,16 @@ Available methods for SVs: 18. Germline and somatic variant benchmarking using Truvari ([truvari bench](https://github.com/acenglish/truvari/wiki/bench)) 19. Germline and somatic variant benchmarking using SVanalyzer ([svanalyzer benchmark](https://github.com/nhansen/SVanalyzer/blob/master/docs/svbenchmark.rst)) +> [!NOTE] +> Please note that there is no somatic spesific tool for SV benchmarking in this pipeline. + Available methods for CNVs: 20. Germline and somatic variant benchmarking using Wittyer ([witty.er](https://github.com/Illumina/witty.er/tree/master)) +> [!NOTE] +> Please note that there is no somatic spesific tool for CNV benchmarking in this pipeline. + Available methods for SNVs and INDELs: 21. Germline variant benchmarking using RTG-tools ([rtg vcfeval](https://realtimegenomics.com/products/rtg-tools)) @@ -102,10 +108,6 @@ The generation of comprehensive report that consolidates all benchmarking result 29. Create visual HTML report for the integration of NCBENCH ([datavzrd](https://datavzrd.github.io/docs/index.html)) 30. Apply MultiQC to visualize results - -1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) - ## Usage > [!NOTE] @@ -160,7 +162,10 @@ nf-core/variantbenchmarking was originally written by Kübra Narcı ([@kubranarc We thank the following people for their extensive assistance in the development of this pipeline: - Nicolas Vannieuwkerke ([@nvnienwk](https://github.com/nvnieuwk)), -- Maxime Garcia ([@maxulysse](https://github.com/maxulysse)) +- Maxime Garcia ([@maxulysse](https://github.com/maxulysse)), +- Sameesh Kher ([@khersameesh24](https://github.com/khersameesh24)) +- Florian Heyl ([@heylf](https://github.com/heyl)) +- Elad Herz ([@EladH1](https://github.com/EladH1)) ## Acknowledgements diff --git a/conf/modules.config b/conf/modules.config index 5b834e3..5d0153f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -375,14 +375,6 @@ process { enabled: false ] } - withName: BAMSURGEON_EVALUATOR { - ext.prefix = {"${meta.id}.${params.truth_id}.${meta.caller}"} - publishDir = [ - path: {"${params.outdir}/${params.variant_type}/${meta.id}/benchmarks/bamsurgeon"}, - pattern: "*{.vcf,stats}", - mode: params.publish_dir_mode - ] - } // report_benchmark_statistics diff --git a/docs/output.md b/docs/output.md index 40fe887..d975421 100644 --- a/docs/output.md +++ b/docs/output.md @@ -33,7 +33,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d
    Output files -- `mpreprocesses/` +- `preprocess/` - `*.vcf.gz`: The standardized and normalized VCF files
    @@ -47,7 +47,7 @@ Outputs from standardization, normalization and filtration processes saved. When - ## `liftover/` -- `preporocesses/liftover/` +- `preproces/liftover/` - `*.vcf.gz`: Lifted over variants - `*.bed`: Lifted over regions @@ -75,6 +75,8 @@ bcftools stats applied into all variant types while survivor stats is only avail
    Output files +-`benchmarks/` + - `truvari/` - `*.fn.vcf.gz` : False negative calls from comparison - `*.fn.vcf.gz.tbi` : False negative calls from comparison - index file @@ -137,78 +139,83 @@ Benchmark results are created separately for each test vcf and for each method u
    Output files -- `comparisons/` - - `small/` - - `rtgtools.small.FN.csv`: Summarizes and compares variants from the baseline VCF of rtgtools which were not correctly called - - `rtgtools.small.FP.csv`: Summarizes and compares variants from the calls VCF of rtgtools which do not agree with baseline variant - - `rtgtools.small.TP_base.csv`: Summarizes and compares variants from the baseline VCF of rtgtools which were correctly called - - `rtgtools.small.TP_comp.csv`: Summarizes and compares variants from the calls VCF of rtgtools which do agree with baseline variant - - `sv/` - - `svbenchmark.sv.FN.csv`: Summarizes and compares variants from the baseline VCF of svbenchmark which were not correctly called - - `svbenchmark.sv.FP.csv`: Summarizes and compares variants from the calls VCF of svbenchmark which do not agree with baseline variant - - `truvari.sv.FN.csv`: Summarizes and compares variants from the baseline VCF of truvari which were not correctly called - - `truvari.sv.FP.csv`: Summarizes and compares variants from the calls VCF of truvari which do not agree with baseline variant - - `truvari.sv.TP_base.csv`: Summarizes and compares variants from the baseline VCF of truvari which were correctly called - - `truvari.sv.TP_comp.csv`: Summarizes and compares variants from the calls VCF of truvari which do agree with baseline variant -- `plots/` - - `cnv/` - - `wittyer/` - - `Base_metric_by_tool_wittyer.png`: Summary plot for callers on precision, recall and F1 per base in wittyer - - `Base_variants_by_tool_wittyer.png`: Summary plot for callers on TP, FP and FN numbers per base in wittyer - - `Event_metric_by_tool_wittyer.png`: Summary plot for callers on precision, recall and F1 per event in wittyer - - `Event_variants_by_tool_wittyer.png`: Summary plot for callers on TP, FP and FN numbers per ecent in wittyer - - `sv/` - - `truvari/` - - `metric_by_tool_truvari.png`: Summary plot for callers on precision, recall and F1 in truvari - - `variants_by_tool_truvari.png`: Summary plot for callers on TP, FP and FN numbers in truvari - - `svbenchmark/` - - `metric_by_tool_svbenchmark.png`: Summary plot for callers on precision, recall and F1 in svbenchmark - - `variants_by_tool_svbenchmark.png`: Summary plot for callers on TP, FP and FN numbers in svbenchmark - - `small/` - - `happy/` - - `INDEL_ALL_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of all INDELs in happy - - `INDEL_ALL_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of all INDELs in happy - - `INDEL_PASS_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of only PASSed INDELs in happy - - `INDEL_PASS_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of only PASSed INDELs in happy - - `SNP_ALL_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of all SNPs in happy - - `SNP_ALL_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of all SNPs in happy - - `SNP_PASS_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of only PASSed SNPs in happy - - `SNP_PASS_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of only PASSed SNPs in happy - - `rtgtools/` - - `metric_by_tool_rtgtools.png`: Summary plot for callers on precision, recall and F1 in rtgtools - - `variants_by_tool_rtgtools.png`: Summary plot for callers on TP, FP and FN numbers in rtgtools - - `indel/` - - `sompy/` - - `metric_by_tool_sompy.png`: Summary plot for callers on precision, recall and F1 of indels in sompy - - `variants_by_tool_sompy.png`: Summary plot for callers on TP, FP and FN numbers of indels in sompy - - `snv/` - - `sompy/` - - `metric_by_tool_sompy.png`: Summary plot for callers on precision, recall and F1 of SNVs in sompy - - `variants_by_tool_sompy.png`: Summary plot for callers on TP, FP and FN numbers of SNVs in sompy -- `tables/` - - `cnv/` - - `wittyer.cnv.summary.csv`: Summary of performance stats from callers - - `sv/` - - `truvari.sv.summary.csv`: Summary of performance stats from callers - - `svbenchmark.sv.summary.csv`: Summary of performance stats from callers - - `small/` - - `happy.sv.summary.csv`: Summary of performance stats from callers - - `rtgtools.sv.summary.csv`: Summary of performance stats from callers - - `indel/` - - `sompy.indel.summary.csv`: Summary of performance stats from callers - - `sompy.indel.regions.csv`: Summary of performance stats split by region bins from callers - - `snv/` - - `sompy.snv.summary.csv`: Summary of performance stats from callers - - `sompy.snv.regions.csv`: Summary of performance stats split by region bins from callers +- `summary/` + + - `comparisons/` + - `small/` + - `rtgtools.small.FN.csv`: Summarizes and compares variants from the baseline VCF of rtgtools which were not correctly called + - `rtgtools.small.FP.csv`: Summarizes and compares variants from the calls VCF of rtgtools which do not agree with baseline variant + - `rtgtools.small.TP_base.csv`: Summarizes and compares variants from the baseline VCF of rtgtools which were correctly called + - `rtgtools.small.TP_comp.csv`: Summarizes and compares variants from the calls VCF of rtgtools which do agree with baseline variant + - `sv/` + - `svbenchmark.sv.FN.csv`: Summarizes and compares variants from the baseline VCF of svbenchmark which were not correctly called + - `svbenchmark.sv.FP.csv`: Summarizes and compares variants from the calls VCF of svbenchmark which do not agree with baseline variant + - `truvari.sv.FN.csv`: Summarizes and compares variants from the baseline VCF of truvari which were not correctly called + - `truvari.sv.FP.csv`: Summarizes and compares variants from the calls VCF of truvari which do not agree with baseline variant + - `truvari.sv.TP_base.csv`: Summarizes and compares variants from the baseline VCF of truvari which were correctly called + - `truvari.sv.TP_comp.csv`: Summarizes and compares variants from the calls VCF of truvari which do agree with baseline variant + - `plots/` + - `cnv/` + - `wittyer/` + - `Base_metric_by_tool_wittyer.png`: Summary plot for callers on precision, recall and F1 per base in wittyer + - `Base_variants_by_tool_wittyer.png`: Summary plot for callers on TP, FP and FN numbers per base in wittyer + - `Event_metric_by_tool_wittyer.png`: Summary plot for callers on precision, recall and F1 per event in wittyer + - `Event_variants_by_tool_wittyer.png`: Summary plot for callers on TP, FP and FN numbers per ecent in wittyer + - `sv/` + - `truvari/` + - `metric_by_tool_truvari.png`: Summary plot for callers on precision, recall and F1 in truvari + - `variants_by_tool_truvari.png`: Summary plot for callers on TP, FP and FN numbers in truvari + - `svbenchmark/` + - `metric_by_tool_svbenchmark.png`: Summary plot for callers on precision, recall and F1 in svbenchmark + - `variants_by_tool_svbenchmark.png`: Summary plot for callers on TP, FP and FN numbers in svbenchmark + - `small/` + - `happy/` + - `INDEL_ALL_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of all INDELs in happy + - `INDEL_ALL_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of all INDELs in happy + - `INDEL_PASS_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of only PASSed INDELs in happy + - `INDEL_PASS_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of only PASSed INDELs in happy + - `SNP_ALL_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of all SNPs in happy + - `SNP_ALL_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of all SNPs in happy + - `SNP_PASS_metric_by_tool_happy.png`: Summary plot for callers on precision, recall and F1 of only PASSed SNPs in happy + - `SNP_PASS_variants_by_tool_happy.png`: Summary plot for callers on TP, FP and FN numbers of only PASSed SNPs in happy + - `rtgtools/` + - `metric_by_tool_rtgtools.png`: Summary plot for callers on precision, recall and F1 in rtgtools + - `variants_by_tool_rtgtools.png`: Summary plot for callers on TP, FP and FN numbers in rtgtools + - `indel/` + - `sompy/` + - `metric_by_tool_sompy.png`: Summary plot for callers on precision, recall and F1 of indels in sompy + - `variants_by_tool_sompy.png`: Summary plot for callers on TP, FP and FN numbers of indels in sompy + - `snv/` + - `sompy/` + - `metric_by_tool_sompy.png`: Summary plot for callers on precision, recall and F1 of SNVs in sompy + - `variants_by_tool_sompy.png`: Summary plot for callers on TP, FP and FN numbers of SNVs in sompy + - `tables/` + - `cnv/` + - `wittyer.cnv.summary.csv`: Summary of performance stats from callers + - `sv/` + - `truvari.sv.summary.csv`: Summary of performance stats from callers + - `svbenchmark.sv.summary.csv`: Summary of performance stats from callers + - `small/` + - `happy.sv.summary.csv`: Summary of performance stats from callers + - `rtgtools.sv.summary.csv`: Summary of performance stats from callers + - `indel/` + - `sompy.indel.summary.csv`: Summary of performance stats from callers + - `sompy.indel.regions.csv`: Summary of performance stats split by region bins from callers + - `snv/` + - `sompy.snv.summary.csv`: Summary of performance stats from callers + - `sompy.snv.regions.csv`: Summary of performance stats split by region bins from callers + - ## `datavzrd/`
    Output files -- benchmark tool - - static - - test - - index.html: HTML file to open in the browser for datavzrd spedific tables and visualizations. +- `summary/` + - `datavzrd/` + - benchmark_tool + - `static` : all static files necessary for datavzrd visualization + - `test` : all data and plots necessary for datavzrd visualization + - `index.html`: HTML file to open in the browser for datavzrd spesific tables and visualizations. ### References diff --git a/modules/local/bamsurgeon/evaluator/main.nf b/modules/local/bamsurgeon/evaluator/main.nf deleted file mode 100644 index f9416f5..0000000 --- a/modules/local/bamsurgeon/evaluator/main.nf +++ /dev/null @@ -1,46 +0,0 @@ -process BAMSURGEON_EVALUATOR { - tag "$meta.id" - label 'process_low' - - conda "" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'docker://lethalfang/bamsurgeon:1.2': - 'lethalfang/bamsurgeon:1.2' }" - - input: - tuple val(meta), path(vcf), path(tbi), path(truth_vcf), path(truth_tbi) - tuple val(meta2), path(fasta) - tuple val(meta3), path(fai) - - output: - tuple val(meta),path("*falsepositives.vcf") , emit: fp - tuple val(meta),path("*truepositives.vcf") , emit: tp - tuple val(meta),path("*falsenegatives.vcf") , emit: fn - tuple val(meta),path("*.stats") , emit: stats - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def muttype = meta.vartype.contains("snv") ? "SNV" : meta.vartype.contains("indel") ? "INDEL" : meta.vartype.contains("sv") ? "SV" : "" - - """ - python3 /usr/local/bamsurgeon/scripts/evaluator.py \\ - -v $vcf \\ - -t $truth_vcf \\ - -f $fasta \\ - -m $muttype \\ - $args \\ - --fp ${prefix}.falsepositives.vcf \\ - --tp ${prefix}.truepositives.vcf \\ - --fn ${prefix}.falsenegatives.vcf > ${prefix}.stats - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - bamsurgeon: v1.2 - END_VERSIONS - """ -} diff --git a/modules/local/custom/create_datavzrd_input/environment.yml b/modules/local/custom/create_datavzrd_input/environment.yml new file mode 100644 index 0000000..c779485 --- /dev/null +++ b/modules/local/custom/create_datavzrd_input/environment.yml @@ -0,0 +1,7 @@ +channels: + - conda-forge + - bioconda +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.8 + - conda-forge::tar=1.34 diff --git a/modules/local/custom/create_datavzrd_input/main.nf b/modules/local/custom/create_datavzrd_input/main.nf index 5bf39e3..1dc0f3d 100644 --- a/modules/local/custom/create_datavzrd_input/main.nf +++ b/modules/local/custom/create_datavzrd_input/main.nf @@ -2,7 +2,7 @@ process CREATE_DATAVZRD_INPUT { tag "$meta.id" label 'process_single' - conda "conda-forge::tar=1.34" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'quay.io/nf-core/ubuntu:20.04' }" @@ -18,10 +18,20 @@ process CREATE_DATAVZRD_INPUT { #!/bin/bash cat "$template" | sed "s|CSVPATH|$csv|g" > config.yaml + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*(GNU coreutils) //; s/ Copyright.*\$//') + END_VERSIONS """ stub: """ touch config.yaml + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cat: \$(echo \$(cat --version 2>&1) | sed 's/^.*(GNU coreutils) //; s/ Copyright.*\$//') + END_VERSIONS """ } diff --git a/modules/local/custom/publish_processed_vcf/environment.yml b/modules/local/custom/publish_processed_vcf/environment.yml index 203c32c..c779485 100644 --- a/modules/local/custom/publish_processed_vcf/environment.yml +++ b/modules/local/custom/publish_processed_vcf/environment.yml @@ -1,8 +1,7 @@ -name: publish_processed_vcf channels: - conda-forge - bioconda - - defaults dependencies: - - bioconda::tabix=1.11 - - bioconda::htslib=1.19.1 + - conda-forge::grep=3.11 + - conda-forge::sed=4.8 + - conda-forge::tar=1.34 diff --git a/modules/local/custom/publish_processed_vcf/main.nf b/modules/local/custom/publish_processed_vcf/main.nf index d13313c..e3988da 100644 --- a/modules/local/custom/publish_processed_vcf/main.nf +++ b/modules/local/custom/publish_processed_vcf/main.nf @@ -2,16 +2,17 @@ process PUBLISH_PROCESSED_VCF { tag "$meta.id" label 'process_single' - conda "" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/tabix:1.11--hdfd78af_0' : - 'biocontainers/tabix:1.11--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'quay.io/nf-core/ubuntu:20.04' }" input: tuple val(meta), path(vcf), path(index) output: tuple val(meta),path("*.vcf.gz"), path("*.vcf.gz.tbi"), emit: gz_tbi + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -24,6 +25,10 @@ process PUBLISH_PROCESSED_VCF { cp $vcf ${prefix}.vcf.gz cp $index ${prefix}.vcf.gz.tbi + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cp: \$(echo \$(cp --version 2>&1) | sed 's/^.*(GNU coreutils) //; s/ Copyright.*\$//') + END_VERSIONS """ stub: def prefix = task.ext.prefix ?: "${meta.id}" @@ -31,5 +36,9 @@ process PUBLISH_PROCESSED_VCF { touch ${prefix}.vcf.gz touch ${prefix}.vcf.gz.tbi + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cp: \$(echo \$(cp --version 2>&1) | sed 's/^.*(GNU coreutils) //; s/ Copyright.*\$//') + END_VERSIONS """ } diff --git a/modules/local/custom/sort_bed/environment.yml b/modules/local/custom/sort_bed/environment.yml new file mode 100644 index 0000000..c779485 --- /dev/null +++ b/modules/local/custom/sort_bed/environment.yml @@ -0,0 +1,7 @@ +channels: + - conda-forge + - bioconda +dependencies: + - conda-forge::grep=3.11 + - conda-forge::sed=4.8 + - conda-forge::tar=1.34 diff --git a/modules/local/custom/sort_bed/main.nf b/modules/local/custom/sort_bed/main.nf index 58e3a18..f02712b 100644 --- a/modules/local/custom/sort_bed/main.nf +++ b/modules/local/custom/sort_bed/main.nf @@ -2,7 +2,7 @@ process SORT_BED { tag "$meta.id" label 'process_low' - conda "conda-forge::tar=1.34" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'quay.io/nf-core/ubuntu:20.04' }" @@ -12,6 +12,7 @@ process SORT_BED { output: tuple val(meta), path("*.bed"), emit: bed + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -23,12 +24,22 @@ process SORT_BED { // sorts the positions by -k1,1 -k2,2n """ sort -k1,1 -k2,2n $bed > ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sort: \$(echo \$(sort --version 2>&1) | sed 's/^.*(GNU coreutils) //; s/ Copyright.*\$//') + END_VERSIONS """ stub: def prefix = task.ext.prefix ?: "${meta.id}" """ touch ${prefix}.bed + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sort: \$(echo \$(sort --version 2>&1) | sed 's/^.*(GNU coreutils) //; s/ Copyright.*\$//') + END_VERSIONS """ } diff --git a/nextflow.config b/nextflow.config index ca1e0b0..6fb6ea6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -194,16 +194,16 @@ profiles { ] } } - test { includeConfig 'conf/test.config' } - germline_small { includeConfig 'conf/tests/germline_small.config'} - germline_sv { includeConfig 'conf/tests/germline_sv.config' } - somatic_snv { includeConfig 'conf/tests/somatic_snv.config' } - somatic_indel { includeConfig 'conf/tests/somatic_indel.config' } - somatic_sv { includeConfig 'conf/tests/somatic_sv.config' } - liftover_test { includeConfig 'conf/tests/liftover_test.config' } + test { includeConfig 'conf/test.config' } + germline_small { includeConfig 'conf/tests/germline_small.config' } + germline_sv { includeConfig 'conf/tests/germline_sv.config' } + somatic_snv { includeConfig 'conf/tests/somatic_snv.config' } + somatic_indel { includeConfig 'conf/tests/somatic_indel.config' } + somatic_sv { includeConfig 'conf/tests/somatic_sv.config' } + liftover_test { includeConfig 'conf/tests/liftover_test.config' } liftover_truth { includeConfig 'conf/tests/liftover_truth.config' } - test_germline_full { includeConfig 'conf/test_germline_full.config' } - test_somatic_full { includeConfig 'conf/test_somatic_full.config' } + test_full { includeConfig 'conf/test_full.config' } + test_somatic_full { includeConfig 'conf/test_full_somatic.config' } } // Load nf-core custom profiles from different Institutions diff --git a/subworkflows/local/liftover_vcfs/main.nf b/subworkflows/local/liftover_vcfs/main.nf index c357af7..e5a4917 100644 --- a/subworkflows/local/liftover_vcfs/main.nf +++ b/subworkflows/local/liftover_vcfs/main.nf @@ -30,7 +30,7 @@ workflow LIFTOVER_VCFS { fasta ) dictionary = PICARD_CREATESEQUENCEDICTIONARY.out.reference_dict - versions = versions.mix(PICARD_CREATESEQUENCEDICTIONARY.out.versions.first()) + versions = versions.mix(PICARD_CREATESEQUENCEDICTIONARY.out.versions) } // Use picard liftovervcf tool to convert vcfs @@ -40,14 +40,14 @@ workflow LIFTOVER_VCFS { fasta, chain ) - versions = versions.mix(PICARD_LIFTOVERVCF.out.versions.first()) + versions = versions.mix(PICARD_LIFTOVERVCF.out.versions) vcf_ch = PICARD_LIFTOVERVCF.out.vcf_lifted // reformat header, convert PS TYPE integer to string after liftover REFORMAT_HEADER( vcf_ch.map{meta, vcf -> tuple(meta, vcf, [])} ) - versions = versions.mix(REFORMAT_HEADER.out.versions.first()) + versions = versions.mix(REFORMAT_HEADER.out.versions) // rename chr after liftover BCFTOOLS_RENAME_CHR( @@ -55,24 +55,26 @@ workflow LIFTOVER_VCFS { rename_chr ) vcf_ch = BCFTOOLS_RENAME_CHR.out.vcf + versions = versions.mix(BCFTOOLS_RENAME_CHR.out.versions) // liftover high confidence bed file if given UCSC_LIFTOVER( ch_bed.map{file -> tuple([id: params.truth_id], file)}, chain.map{_meta, file -> file} ) - versions = versions.mix(UCSC_LIFTOVER.out.versions.first()) + versions = versions.mix(UCSC_LIFTOVER.out.versions) // sort bed file SORT_BED( UCSC_LIFTOVER.out.lifted ) + versions = versions.mix(SORT_BED.out.versions) // merge the intersected regions BEDTOOLS_MERGE( SORT_BED.out.bed ) - versions = versions.mix(BEDTOOLS_MERGE.out.versions.first()) + versions = versions.mix(BEDTOOLS_MERGE.out.versions) bed_ch = BEDTOOLS_MERGE.out.bed emit: diff --git a/subworkflows/local/prepare_vcfs_truth/main.nf b/subworkflows/local/prepare_vcfs_truth/main.nf index 60cd3c5..2b49d29 100644 --- a/subworkflows/local/prepare_vcfs_truth/main.nf +++ b/subworkflows/local/prepare_vcfs_truth/main.nf @@ -36,7 +36,7 @@ workflow PREPARE_VCFS_TRUTH { rename_chr, dictionary ) - versions = versions.mix(LIFTOVER_VCFS.out.versions.first()) + versions = versions.mix(LIFTOVER_VCFS.out.versions) truth_ch = LIFTOVER_VCFS.out.vcf_ch high_conf_ch = LIFTOVER_VCFS.out.bed_ch.map{ _meta, bed -> [bed]} } @@ -49,7 +49,7 @@ workflow PREPARE_VCFS_TRUTH { }, fai ) - versions = versions.mix(BCFTOOLS_REHEADER_TRUTH.out.versions.first()) + versions = versions.mix(BCFTOOLS_REHEADER_TRUTH.out.versions) BCFTOOLS_REHEADER_TRUTH.out.vcf.join(BCFTOOLS_REHEADER_TRUTH.out.index) .set{vcf_ch} @@ -61,7 +61,7 @@ workflow PREPARE_VCFS_TRUTH { vcf_ch, fasta ) - versions = versions.mix(BCFTOOLS_SPLIT_MULTI.out.versions.first()) + versions = versions.mix(BCFTOOLS_SPLIT_MULTI.out.versions) BCFTOOLS_SPLIT_MULTI.out.vcf.join(BCFTOOLS_SPLIT_MULTI.out.tbi, by:0) .set{vcf_ch} @@ -85,7 +85,7 @@ workflow PREPARE_VCFS_TRUTH { vcf_ch, fasta ) - versions = versions.mix(BCFTOOLS_NORM.out.versions.first()) + versions = versions.mix(BCFTOOLS_NORM.out.versions) BCFTOOLS_NORM.out.vcf.join(BCFTOOLS_NORM.out.tbi, by:0) .set{vcf_ch} @@ -94,6 +94,8 @@ workflow PREPARE_VCFS_TRUTH { PUBLISH_PROCESSED_VCF( vcf_ch ) + versions = versions.mix(PUBLISH_PROCESSED_VCF.out.versions) + emit: vcf_ch // channel: [val(meta), vcf, tbi] high_conf_ch // channel: [val(meta), bed] diff --git a/subworkflows/local/small_somatic_benchmark/main.nf b/subworkflows/local/small_somatic_benchmark/main.nf index 6e9c220..0665083 100644 --- a/subworkflows/local/small_somatic_benchmark/main.nf +++ b/subworkflows/local/small_somatic_benchmark/main.nf @@ -3,7 +3,6 @@ // include { HAPPY_SOMPY } from '../../../modules/nf-core/happy/sompy/main' -include { BAMSURGEON_EVALUATOR } from '../../../modules/local/bamsurgeon/evaluator' workflow SMALL_SOMATIC_BENCHMARK { take: @@ -36,26 +35,6 @@ workflow SMALL_SOMATIC_BENCHMARK { .set{ report } summary_reports = summary_reports.mix(report) } - - // not working for now - if (params.method.contains('bamsurgeon')){ - - BAMSURGEON_EVALUATOR( - input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, _bed -> - [ meta, vcf, tbi, truth_vcf, truth_tbi ] - }, - fasta, - fai - ) - versions = versions.mix(BAMSURGEON_EVALUATOR.out.versions.first()) - - BAMSURGEON_EVALUATOR.out.stats - .map { _meta, file -> tuple([vartype: params.variant_type] + [benchmark_tool: "bamsurgeon"], file) } - .groupTuple() - .set{ report } - summary_reports = summary_reports.mix(report) - } - emit: summary_reports // channel: [val(meta), reports] versions // channel: [versions.yml] diff --git a/subworkflows/local/sv_somatic_benchmark/main.nf b/subworkflows/local/sv_somatic_benchmark/main.nf deleted file mode 100644 index 65bae27..0000000 --- a/subworkflows/local/sv_somatic_benchmark/main.nf +++ /dev/null @@ -1,40 +0,0 @@ -// -// SOMATIC: SUBWORKFLOW FOR SMALL SOMATIC VARIANTS -// - -include { BAMSURGEON_EVALUATOR } from '../../../modules/local/bamsurgeon/evaluator' - -workflow SV_SOMATIC_BENCHMARK { - take: - input_ch // channel: [val(meta), test_vcf, test_index, truth_vcf, truth_index, bed] - fasta // reference channel [val(meta), ref.fa] - fai // reference channel [val(meta), ref.fa.fai] - - main: - - versions = Channel.empty() - summary_reports = Channel.empty() - - - if (params.method.contains('bamsurgeon')){ - - BAMSURGEON_EVALUATOR( - input_ch.map { meta, vcf, tbi, truth_vcf, truth_tbi, bed -> - [ meta, vcf, tbi, truth_vcf, truth_tbi ] - }, - fasta, - fai - ) - versions = versions.mix(BAMSURGEON_EVALUATOR.out.versions.first()) - - BAMSURGEON_EVALUATOR.out.stats - .map { meta, file -> tuple([vartype: meta.vartype] + [benchmark_tool: "bamsurgeon"], file) } - .groupTuple() - .set{ report } - summary_reports = summary_reports.mix(report) - } - - emit: - summary_reports // channel: [val(meta), reports] - versions // channel: [versions.yml] -} diff --git a/subworkflows/local/sv_vcf_conversion/main.nf b/subworkflows/local/sv_vcf_conversion/main.nf index 0c9af4b..5850cae 100644 --- a/subworkflows/local/sv_vcf_conversion/main.nf +++ b/subworkflows/local/sv_vcf_conversion/main.nf @@ -1,5 +1,3 @@ -import groovy.io.FileType - // // SV_VCF_CONVERSIONS: SUBWORKFLOW to apply tool spesific conversions // diff --git a/tests/somatic_indel.nf.test.snap b/tests/somatic_indel.nf.test.snap index d1ca379..75910a1 100644 --- a/tests/somatic_indel.nf.test.snap +++ b/tests/somatic_indel.nf.test.snap @@ -144,6 +144,6 @@ "nf-test": "0.9.2", "nextflow": "24.10.4" }, - "timestamp": "2025-01-29T17:06:57.061841561" + "timestamp": "2025-02-05T08:52:23.622412294" } } \ No newline at end of file From 845a021f77557cc6ca75efa88a289462094bdb59 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 17 Feb 2025 09:49:59 +0000 Subject: [PATCH 284/292] update manta_convertinversions --- .github/workflows/ci.yml | 2 +- modules.json | 2 +- .../manta/convertinversion/environment.yml | 6 +- .../nf-core/manta/convertinversion/main.nf | 13 +++ .../nf-core/manta/convertinversion/meta.yml | 70 +++++++++----- .../manta/convertinversion/tests/main.nf.test | 92 +++++++++++++++++++ .../convertinversion/tests/main.nf.test.snap | 85 +++++++++++++++++ .../manta/convertinversion/tests/tags.yml | 2 + 8 files changed, 242 insertions(+), 30 deletions(-) create mode 100644 modules/nf-core/manta/convertinversion/tests/main.nf.test create mode 100644 modules/nf-core/manta/convertinversion/tests/main.nf.test.snap create mode 100644 modules/nf-core/manta/convertinversion/tests/tags.yml diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 4771574..c4ca534 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -47,7 +47,7 @@ jobs: profile: "conda" - isMaster: false profile: "singularity" - shard: [1, 2, 3, 4, 5, 6] + shard: [1, 2, 3, 4, 5] steps: - name: Check out pipeline code uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 diff --git a/modules.json b/modules.json index faee1cb..db37526 100644 --- a/modules.json +++ b/modules.json @@ -72,7 +72,7 @@ }, "manta/convertinversion": { "branch": "master", - "git_sha": "1e2b7fb7106852388610c0360d234b0829eb980e", + "git_sha": "81880787133db07d9b4c1febd152c090eb8325dc", "installed_by": ["modules"] }, "multiqc": { diff --git a/modules/nf-core/manta/convertinversion/environment.yml b/modules/nf-core/manta/convertinversion/environment.yml index c238cf4..3aa535e 100644 --- a/modules/nf-core/manta/convertinversion/environment.yml +++ b/modules/nf-core/manta/convertinversion/environment.yml @@ -1,9 +1,9 @@ -name: manta_convertinversion +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json channels: - conda-forge - bioconda - - defaults dependencies: + - bioconda::htslib=1.16 - bioconda::manta=1.6.0 - bioconda::samtools=1.16.1 - - bioconda::htslib=1.16 diff --git a/modules/nf-core/manta/convertinversion/main.nf b/modules/nf-core/manta/convertinversion/main.nf index dde263d..462b2e5 100644 --- a/modules/nf-core/manta/convertinversion/main.nf +++ b/modules/nf-core/manta/convertinversion/main.nf @@ -33,4 +33,17 @@ process MANTA_CONVERTINVERSION { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' ) END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + echo "" | gzip > ${prefix}.vcf.gz + touch ${prefix}.vcf.gz.tbi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + manta: \$( configManta.py --version ) + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//' ) + END_VERSIONS + """ } diff --git a/modules/nf-core/manta/convertinversion/meta.yml b/modules/nf-core/manta/convertinversion/meta.yml index cd1d3ce..3a70814 100644 --- a/modules/nf-core/manta/convertinversion/meta.yml +++ b/modules/nf-core/manta/convertinversion/meta.yml @@ -1,5 +1,7 @@ name: "manta_convertinversion" -description: Manta calls structural variants (SVs) and indels from mapped paired-end sequencing reads. This script reformats inversions into single inverted sequence junctions which was the format used in Manta versions <= 1.4.0. +description: Manta calls structural variants (SVs) and indels from mapped paired-end + sequencing reads. This script reformats inversions into single inverted sequence + junctions which was the format used in Manta versions <= 1.4.0. keywords: - structural variants - conversion @@ -12,34 +14,52 @@ tools: tool_dev_url: https://github.com/Illumina/manta doi: "10.1093/bioinformatics/btv710" licence: ["GPL v3"] + identifier: biotools:manta_sv input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - vcf: - type: file - description: VCF file produces by Manta - pattern: "*.vcf.gz" + - - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - vcf: + type: file + description: VCF file produces by Manta + pattern: "*.vcf.gz" + - - meta2: + type: map + description: | + Groovy Map containing fasta information + e.g. [ id:'test', single_end:false ] + - fasta: + type: file + description: The reference fasta file + pattern: "*.{fasta,fa,fna}" output: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" - vcf: - type: file - description: VCF file with reformatted inversions - pattern: "*.vcf.gz" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.vcf.gz": + type: file + description: VCF file with reformatted inversions + pattern: "*.vcf.gz" - tbi: - type: file - description: TBI file produces by Manta - pattern: "*.vcf.gz.tbi" + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - "*.vcf.gz.tbi": + type: file + description: TBI file produces by Manta + pattern: "*.vcf.gz.tbi" + - versions: + - versions.yml: + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@FriederikeHanssen" maintainers: diff --git a/modules/nf-core/manta/convertinversion/tests/main.nf.test b/modules/nf-core/manta/convertinversion/tests/main.nf.test new file mode 100644 index 0000000..58adf6e --- /dev/null +++ b/modules/nf-core/manta/convertinversion/tests/main.nf.test @@ -0,0 +1,92 @@ +nextflow_process { + + name "Test Process MANTA_CONVERTINVERSION" + script "../main.nf" + process "MANTA_CONVERTINVERSION" + + tag "modules" + tag "modules_nfcore" + tag "manta" + tag "manta/convertinversion" + tag "manta/tumoronly" + + setup { + run("MANTA_TUMORONLY") { + script "../../tumoronly/main.nf" + process { + """ + input[0] = [ + [ id:'test'], // meta map + file(params.modules_testdata_base_path + "genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram"), + file(params.modules_testdata_base_path + "genomics/homo_sapiens/illumina/cram/test2.paired_end.recalibrated.sorted.cram.crai"), + [], + [] + ] + input[1] = [ + [ id:'fasta' ], + file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/chr21/sequence/genome.fasta") + ] + input[2] = [ + [ id:'fai' ], + file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/chr21/sequence/genome.fasta.fai") + ] + input[3] = [] + """ + } + } + + } + + test("homo_sapiens - vcf, fasta") { + + when { + process { + """ + input[0] = MANTA_TUMORONLY.out.tumor_sv_vcf + input[1] = [ + [ id:'fasta' ], + file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/chr21/sequence/genome.fasta") + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.vcf.collect { it.collect { it instanceof Map ? it : path(it).linesGzip[-7..-3] } }, + process.out.tbi.collect { it.collect { it instanceof Map ? it : file(it).name } }, + process.out.versions + ).match() } + ) + } + + } + + test("homo_sapiens - vcf, fasta - stub") { + + options "-stub" + + when { + process { + """ + input[0] = MANTA_TUMORONLY.out.tumor_sv_vcf + input[1] = [ + [ id:'fasta' ], + file(params.modules_testdata_base_path + "genomics/homo_sapiens/genome/chr21/sequence/genome.fasta") + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match("stub") } + ) + } + + } + +} diff --git a/modules/nf-core/manta/convertinversion/tests/main.nf.test.snap b/modules/nf-core/manta/convertinversion/tests/main.nf.test.snap new file mode 100644 index 0000000..0fba8d6 --- /dev/null +++ b/modules/nf-core/manta/convertinversion/tests/main.nf.test.snap @@ -0,0 +1,85 @@ +{ + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,d9a3c2c54bad36a8310d42eae3d57801" + ], + "tbi": [ + [ + { + "id": "test" + }, + "test.vcf.gz.tbi:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "vcf": [ + [ + { + "id": "test" + }, + "test.vcf.gz:md5,68b329da9893e34099c7d8ad5cb9c940" + ] + ], + "versions": [ + "versions.yml:md5,d9a3c2c54bad36a8310d42eae3d57801" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.03.0" + }, + "timestamp": "2024-05-06T10:26:25.996091364" + }, + "homo_sapiens - vcf, fasta": { + "content": [ + [ + [ + { + "id": "test" + }, + [ + "##FILTER=", + "##ALT=", + "##ALT=", + "##ALT=", + "##ALT=" + ] + ] + ], + [ + [ + { + "id": "test" + }, + "test.vcf.gz.tbi" + ] + ], + [ + "versions.yml:md5,d9a3c2c54bad36a8310d42eae3d57801" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.03.0" + }, + "timestamp": "2024-05-06T13:34:11.206457972" + } +} \ No newline at end of file diff --git a/modules/nf-core/manta/convertinversion/tests/tags.yml b/modules/nf-core/manta/convertinversion/tests/tags.yml new file mode 100644 index 0000000..14d94b2 --- /dev/null +++ b/modules/nf-core/manta/convertinversion/tests/tags.yml @@ -0,0 +1,2 @@ +manta/convertinversion: + - "modules/nf-core/manta/convertinversion/**" From b7354d8fd681b24cf5a457676a9104edff58df54 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 17 Feb 2025 15:07:28 +0000 Subject: [PATCH 285/292] copy from fix --- .github/workflows/download_pipeline.yml | 28 ++++++++++++------------- 1 file changed, 14 insertions(+), 14 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index ab06316..1df0fa7 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -12,14 +12,6 @@ on: required: true default: "dev" pull_request: - types: - - opened - - edited - - synchronize - branches: - - main - - master - pull_request_target: branches: - main - master @@ -29,7 +21,7 @@ env: jobs: configure: - runs-on: ubuntu-latest + runs-on: ubuntu-latest{% raw %} outputs: REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }} REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }} @@ -40,7 +32,7 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT" echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT" - echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT" + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT{% endraw %}" download: runs-on: ubuntu-latest @@ -73,7 +65,7 @@ jobs: - name: Download the pipeline env: - NXF_SINGULARITY_CACHEDIR: ./singularity_container_images + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images{% raw %} run: | nf-core pipelines download ${{ needs.configure.outputs.REPO_LOWERCASE }} \ --revision ${{ needs.configure.outputs.REPO_BRANCH }} \ @@ -85,10 +77,10 @@ jobs: --download-configuration 'yes' - name: Inspect download - run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} + run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}{% endraw %} - name: Inspect container images - run: tree ./singularity_container_images | tee ./container_initial + run: tree ./singularity_container_images | tee ./container_initial{% if test_config %}{% raw %} - name: Count the downloaded number of container images id: count_initial @@ -120,6 +112,7 @@ jobs: echo "IMAGE_COUNT_AFTER=$image_count" >> "$GITHUB_OUTPUT" - name: Compare container image counts + id: count_comparison run: | if [ "${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }}" -ne "${{ steps.count_afterwards.outputs.IMAGE_COUNT_AFTER }}" ]; then initial_count=${{ steps.count_initial.outputs.IMAGE_COUNT_INITIAL }} @@ -131,4 +124,11 @@ jobs: exit 1 else echo "The pipeline can be downloaded successfully!" - fi + fi{% endraw %} + + - name: Upload Nextflow logfile for debugging purposes + uses: actions/upload-artifact@v4 + with: + name: nextflow_logfile.txt + path: .nextflow.log* + include-hidden-files: true{% endif %} From d2f62101ffa6c038b200cbee2d52a024bf6bc043 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 17 Feb 2025 16:17:51 +0100 Subject: [PATCH 286/292] Update .github/workflows/download_pipeline.yml MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 1df0fa7..73be997 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -21,7 +21,7 @@ env: jobs: configure: - runs-on: ubuntu-latest{% raw %} + runs-on: ubuntu-latest outputs: REPO_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPO_LOWERCASE }} REPOTITLE_LOWERCASE: ${{ steps.get_repo_properties.outputs.REPOTITLE_LOWERCASE }} From c908632d7962847eb22a48dd7e845b0b55d78e73 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 17 Feb 2025 16:17:58 +0100 Subject: [PATCH 287/292] Update .github/workflows/download_pipeline.yml MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 73be997..0782069 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -32,7 +32,7 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> "$GITHUB_OUTPUT" echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> "$GITHUB_OUTPUT" - echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT{% endraw %}" + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> "$GITHUB_OUTPUT" download: runs-on: ubuntu-latest From 2872d217321b2d9b9122f260fc85c9d59fd1a3fc Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 17 Feb 2025 16:18:04 +0100 Subject: [PATCH 288/292] Update .github/workflows/download_pipeline.yml MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 0782069..75d3205 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -77,7 +77,7 @@ jobs: --download-configuration 'yes' - name: Inspect download - run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }}{% endraw %} + run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} - name: Inspect container images run: tree ./singularity_container_images | tee ./container_initial{% if test_config %}{% raw %} From 3b84b09dc53df55dc6b9c9582eca69fb13da4b15 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 17 Feb 2025 16:18:11 +0100 Subject: [PATCH 289/292] Update .github/workflows/download_pipeline.yml MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 75d3205..78b88af 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -80,7 +80,7 @@ jobs: run: tree ./${{ needs.configure.outputs.REPOTITLE_LOWERCASE }} - name: Inspect container images - run: tree ./singularity_container_images | tee ./container_initial{% if test_config %}{% raw %} + run: tree ./singularity_container_images | tee ./container_initial - name: Count the downloaded number of container images id: count_initial From 888d00b9d1bbc382e4e0b0d2c40d25f17c31ba22 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Mon, 17 Feb 2025 16:18:17 +0100 Subject: [PATCH 290/292] Update .github/workflows/download_pipeline.yml MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 78b88af..efdf8ab 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -65,7 +65,7 @@ jobs: - name: Download the pipeline env: - NXF_SINGULARITY_CACHEDIR: ./singularity_container_images{% raw %} + NXF_SINGULARITY_CACHEDIR: ./singularity_container_images run: | nf-core pipelines download ${{ needs.configure.outputs.REPO_LOWERCASE }} \ --revision ${{ needs.configure.outputs.REPO_BRANCH }} \ From a9ab8971388d24aa018261e6fa08d0fe4c31ae9f Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Thu, 20 Feb 2025 15:59:55 +0000 Subject: [PATCH 291/292] apply Maximes changes --- bin/add_svtype.py | 61 ---------- bin/check_diff.py | 29 ----- bin/fix_vcf_prefix.py | 2 +- bin/merge_reports.py | 3 + bin/parse_vcf.py | 50 -------- bin/plots.R | 3 + bin/vcf_to_csv.py | 2 +- .../nf-core-variantbenchmarking_sticker.png | Bin 276206 -> 272684 bytes .../nf-core-variantbenchmarking_sticker.svg | 107 ++++++++++++++---- nextflow.config | 2 +- nf-test.config | 2 +- 11 files changed, 92 insertions(+), 169 deletions(-) delete mode 100755 bin/add_svtype.py delete mode 100755 bin/check_diff.py delete mode 100755 bin/parse_vcf.py diff --git a/bin/add_svtype.py b/bin/add_svtype.py deleted file mode 100755 index 6bea45a..0000000 --- a/bin/add_svtype.py +++ /dev/null @@ -1,61 +0,0 @@ -import argparse -import pysam -import logging -import os -import subprocess as sp - -logging.basicConfig( - format='%(levelname)-7s | %(asctime)s | %(message)s', - datefmt='%H:%M:%S') -logger = logging.getLogger(__name__) -logger.setLevel(logging.INFO) - - -parser = argparse.ArgumentParser() -parser.add_argument('--input', required=True, help='Input VCF') -parser.add_argument('--svlength', type=int, required=False, help='SV Lenght', default=50) -args = parser.parse_args() - - - -in_vcf = pysam.VariantFile(args.input) -out_name = os.path.basename(args.input) -if out_name.endswith('.gz'): - out_name = out_name[:-3] -if out_name.endswith('.vcf'): - out_name = out_name[:-4] - -anno_header = in_vcf.header -logger.info('Writing Header') -anno_header.info.add("SVTYPE","1","String","Type of structural variant") -anno_header.info.add("SVLEN",".","Integer","Difference in length between the REF and ALT alleles") - -anno_vcf = pysam.VariantFile('{}.annotated.vcf.gz'.format(out_name), 'w', header=anno_header) - -logger.info('Adding SVTYE/SVLEN to VCF') -counter=0 -for v in in_vcf: - svlen=(len(v.alts[0])-len(v.ref)) - limit=args.svlength - - if (svlen < -1*limit): - v.info.update({'SVTYPE':"DEL"}) - v.info.update({'SVLEN':svlen}) - anno_vcf.write(v) - counter=counter+1 - elif (svlen > limit): - v.info.update({'SVTYPE':"INS"}) - v.info.update({'SVLEN':svlen}) - anno_vcf.write(v) - counter=counter+1 - else: - anno_vcf.write(v) - -in_vcf.close() -anno_vcf.close() -logger.info('Finished adding SVTYE/SVLEN to VCF: {}'.format(counter)) - -sp.run(['tabix', anno_vcf.filename]) -logger.info('VCF indexed') - -logger.info('DONE') diff --git a/bin/check_diff.py b/bin/check_diff.py deleted file mode 100755 index cff223a..0000000 --- a/bin/check_diff.py +++ /dev/null @@ -1,29 +0,0 @@ -#!/usr/bin/env python - -import pysam -from argparse import ArgumentParser - -def compare_vcf(file1, file2): - vcf1 = pysam.VariantFile(file1) - vcf2 = pysam.VariantFile(file2) - - variants1 = {rec.id for rec in vcf1.fetch()} - variants2 = {rec.id for rec in vcf2.fetch()} - - unique_to_file1 = variants1 - variants2 - unique_to_file2 = variants2 - variants1 - - print(f"Unique to {file1}: {unique_to_file1}") - print(f"Unique to {file2}: {unique_to_file2}") - -if __name__ == '__main__': - import os - import sys - - # Parse arguments - parser = ArgumentParser(description='Check differences') - parser.add_argument('file1', help='VCF file') - parser.add_argument('file2', help='Output VCF file') - - args = parser.parse_args() - compare_vcf(args.file1, args.file2) diff --git a/bin/fix_vcf_prefix.py b/bin/fix_vcf_prefix.py index adc0bf2..f9e485d 100755 --- a/bin/fix_vcf_prefix.py +++ b/bin/fix_vcf_prefix.py @@ -1,7 +1,7 @@ #!/usr/bin/env python # Copyright 2024 - GHGA -# Author: Kuebra Narci +# Author: Kuebra Narci - @kubranarci ''' Generates a CSV file from a VCF Expected usage: diff --git a/bin/merge_reports.py b/bin/merge_reports.py index 0fd806b..caae970 100755 --- a/bin/merge_reports.py +++ b/bin/merge_reports.py @@ -1,5 +1,8 @@ #!/usr/bin/env python +# Copyright 2024 - GHGA +# Author: Kuebra Narci - @kubranarci + import pandas as pd import glob import re diff --git a/bin/parse_vcf.py b/bin/parse_vcf.py deleted file mode 100755 index 6444b1d..0000000 --- a/bin/parse_vcf.py +++ /dev/null @@ -1,50 +0,0 @@ -import argparse -import pysam -import logging -import os -import subprocess as sp - -logging.basicConfig( - format='%(levelname)-7s | %(asctime)s | %(message)s', - datefmt='%H:%M:%S') -logger = logging.getLogger(__name__) -logger.setLevel(logging.INFO) - -parser = argparse.ArgumentParser() -parser.add_argument('--vcf', required=True, help='Input VCF') -args = parser.parse_args() - -in_vcf = pysam.VariantFile(args.vcf) -out_name = os.path.basename(args.vcf) -if out_name.endswith('.gz'): - out_name = out_name[:-3] -if out_name.endswith('.vcf'): - out_name = out_name[:-4] - -out_header = in_vcf.header -out_vcf = pysam.VariantFile('{}.resolved.vcf.gz'.format(out_name), 'w', header=out_header) - -logger.info('Only will be sequence resolved, BND, and are filtered out!') -for v in in_vcf: - if v.info['SVTYPE'] == 'BND': - continue - else: - if v.alts[0] == '': - continue - - elif v.alts[0] == '': - continue - - elif v.alts[0] == '': - v.alleles = (fasta_f.fetch(v.chrom, v.pos - 1, v.stop), v.ref) - out_vcf.write(v) - else: - out_vcf.write(v) -fasta_f.close() -in_vcf.close() -out_vcf.close() - -sp.run(['tabix', out_vcf.filename]) -logger.info('VCF indexed') - -logger.info('DONE') diff --git a/bin/plots.R b/bin/plots.R index 6734aef..6bfba66 100755 --- a/bin/plots.R +++ b/bin/plots.R @@ -1,5 +1,8 @@ #!/usr/bin/env Rscript +# Copyright 2024 - GHGA +# Author: Kuebra Narci - @kubranarci + # Load necessary libraries suppressWarnings(library(ggplot2)) suppressWarnings(library(reshape2)) diff --git a/bin/vcf_to_csv.py b/bin/vcf_to_csv.py index eb1fb53..eb50840 100755 --- a/bin/vcf_to_csv.py +++ b/bin/vcf_to_csv.py @@ -1,7 +1,7 @@ #!/usr/bin/env python # Copyright 2024 - GHGA -# Author: Kuebra Narci +# Author: Kuebra Narci - @kubranarci ''' Generates a 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--git a/nextflow.config b/nextflow.config index 6fb6ea6..f83701a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -11,7 +11,7 @@ params { // Input options input = null - outdir = "results" + outdir = null // Option to select analysis type: germline or somatic analysis = null diff --git a/nf-test.config b/nf-test.config index a68d97a..1557379 100644 --- a/nf-test.config +++ b/nf-test.config @@ -10,7 +10,7 @@ config { profile "docker,test" - // load the necessary plugins ?? + // load the necessary plugins plugins { load "nft-utils@0.0.3" } From 8c73664b4342f19226018078b01d1fde3b28c303 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?K=C3=BCbra=20Narc=C4=B1?= Date: Fri, 21 Feb 2025 09:37:51 +0000 Subject: [PATCH 292/292] delete stickers --- .../nf-core-variantbenchmarking_sticker.png | Bin 272684 -> 0 bytes .../nf-core-variantbenchmarking_sticker.svg | 407 ------------------ 2 files changed, 407 deletions(-) delete mode 100644 docs/images/nf-core-variantbenchmarking_sticker.png delete mode 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