-
Notifications
You must be signed in to change notification settings - Fork 750
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
* WIP- add pychopper * meta yml complete * Test and snap * Add stub gzipped format, working version * try remove all blank entries in meta yml * linted * Update main.nf add version as def var
- Loading branch information
1 parent
a33c6de
commit 1436029
Showing
5 changed files
with
224 additions
and
0 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,7 @@ | ||
--- | ||
# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json | ||
channels: | ||
- conda-forge | ||
- bioconda | ||
dependencies: | ||
- "bioconda::pychopper=2.7.10" |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,52 @@ | ||
process PYCHOPPER { | ||
tag "$meta.id" | ||
label 'process_low' | ||
|
||
conda "${moduleDir}/environment.yml" | ||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | ||
'https://depot.galaxyproject.org/singularity/pychopper:2.7.10--pyhdfd78af_0': | ||
'biocontainers/pychopper:2.7.10--pyhdfd78af_0' }" | ||
|
||
input: | ||
tuple val(meta), path(fastq) | ||
|
||
output: | ||
tuple val(meta), path("*.out.fastq.gz"), emit: fastq | ||
path "versions.yml" , emit: versions | ||
|
||
when: | ||
task.ext.when == null || task.ext.when | ||
|
||
script: | ||
def args = task.ext.args ?: '' | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
def PYCHOPPER_VERSION = '2.7.10' | ||
|
||
""" | ||
pychopper \\ | ||
$args \\ | ||
-t $task.cpus \\ | ||
$fastq \\ | ||
${prefix}.out.fastq | ||
gzip -f ${prefix}.out.fastq > ${prefix}.out.fastq.gz | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
pychopper: $PYCHOPPER_VERSION (hard coded- check container used for this module) | ||
END_VERSIONS | ||
""" | ||
|
||
stub: | ||
def args = task.ext.args ?: '' | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
""" | ||
touch ${prefix}.out.fastq | ||
gzip ${prefix}.out.fastq | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
pychopper: 2.7.10 (hard coded- check container used for this module) | ||
END_VERSIONS | ||
""" | ||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,54 @@ | ||
name: "pychopper" | ||
description: Identify, orient and trim nanopore cDNA reads | ||
keywords: | ||
- sort | ||
- trimming | ||
- nanopore | ||
tools: | ||
- "pychopper": | ||
description: "A tool to identify, orient and rescue full length cDNA reads from | ||
nanopore data." | ||
homepage: "https://github.com/epi2me-labs/pychopper" | ||
documentation: "https://github.com/epi2me-labs/pychopper" | ||
tool_dev_url: "https://github.com/epi2me-labs/pychopper" | ||
licence: ["Oxford Nanopore Technologies PLC. Public License Version 1.0"] | ||
identifier: "" | ||
- "gzip": | ||
description: "Gzip reduces the size of the named files using Lempel-Ziv coding | ||
(LZ77)." | ||
documentation: "https://linux.die.net/man/1/gzip" | ||
args_id: "$args3" | ||
identifier: "" | ||
input: | ||
- - meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. `[ id:'sample1', single_end:false ]` | ||
- fastq: | ||
type: file | ||
description: FastQ with reads from long read sequencing e.g. nanopore | ||
pattern: "*.{fastq.gz}" | ||
output: | ||
- fastq: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. [ id:'test', single_end:false ] | ||
pattern: "*.{fastq.gz}" | ||
- "*.out.fastq.gz": | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. [ id:'test', single_end:false ] | ||
pattern: "*.{fastq.gz}" | ||
- versions: | ||
- versions.yml: | ||
type: file | ||
description: File containing software versions | ||
pattern: "versions.yml" | ||
authors: | ||
- "@chriswyatt1" | ||
maintainers: | ||
- "@chriswyatt1" |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,69 @@ | ||
nextflow_process { | ||
|
||
name "Test Process PYCHOPPER" | ||
script "../main.nf" | ||
process "PYCHOPPER" | ||
tag "pychopper" | ||
tag "modules" | ||
tag "modules_nfcore" | ||
|
||
test("pychopper-test") { | ||
|
||
when { | ||
params { | ||
outdir = "$outputDir" | ||
} | ||
process { | ||
""" | ||
input[0] = [ | ||
[id:'test_out' ], // meta map | ||
file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test_2.fastq.gz', checkIfExists: true) | ||
] | ||
""" | ||
} | ||
} | ||
|
||
then { | ||
|
||
def fastq_content = path(process.out.fastq.get(0).get(1)).linesGzip | ||
|
||
assertAll( | ||
{ assert process.success }, | ||
// original pytest checks | ||
{ assert process.out.fastq.get(0).get(1) ==~ ".*/test_out.out.fastq.gz" }, | ||
// additional nf-test checks | ||
// Order of reads is not deterministic, so only assess whether the number of reads is correct | ||
{ assert snapshot( | ||
process.out.fastq, | ||
process.out.versions | ||
).match() } | ||
) | ||
} | ||
} | ||
|
||
test("test-pychopper-stub") { | ||
options '-stub' | ||
|
||
when { | ||
process { | ||
""" | ||
input[0] = [ | ||
[id:'test_out' ], // meta map | ||
file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test.fastq.gz', checkIfExists: true) | ||
] | ||
""" | ||
} | ||
} | ||
|
||
then { | ||
assertAll( | ||
{ assert process.success }, | ||
{ assert snapshot( | ||
process.out.fastq, | ||
process.out.versions | ||
).match() } | ||
) | ||
} | ||
} | ||
|
||
} |
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,42 @@ | ||
{ | ||
"pychopper-test": { | ||
"content": [ | ||
[ | ||
[ | ||
{ | ||
"id": "test_out" | ||
}, | ||
"test_out.out.fastq.gz:md5,04f3003f5cb5a78d90cae351beacb094" | ||
] | ||
], | ||
[ | ||
"versions.yml:md5,1431ed95e3c77f8742c7b014b26e50f5" | ||
] | ||
], | ||
"meta": { | ||
"nf-test": "0.9.2", | ||
"nextflow": "24.10.4" | ||
}, | ||
"timestamp": "2025-02-05T13:57:08.581810556" | ||
}, | ||
"test-pychopper-stub": { | ||
"content": [ | ||
[ | ||
[ | ||
{ | ||
"id": "test_out" | ||
}, | ||
"test_out.out.fastq.gz:md5,d41d8cd98f00b204e9800998ecf8427e" | ||
] | ||
], | ||
[ | ||
"versions.yml:md5,1431ed95e3c77f8742c7b014b26e50f5" | ||
] | ||
], | ||
"meta": { | ||
"nf-test": "0.9.2", | ||
"nextflow": "24.10.4" | ||
}, | ||
"timestamp": "2025-02-05T15:09:38.47491875" | ||
} | ||
} |