From 95df0a8e4ea93c19e8c713b047d1cb316df1a8e6 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 4 Oct 2022 21:52:56 +0000 Subject: [PATCH 001/201] Template update for nf-core/tools version 2.6 --- .github/workflows/awsfulltest.yml | 4 ++ .github/workflows/awstest.yml | 4 ++ .prettierignore | 1 + CITATION.cff | 8 +-- LICENSE | 2 +- README.md | 2 +- assets/adaptivecard.json | 67 +++++++++++++++++++ assets/methods_description_template.yml | 25 +++++++ assets/multiqc_config.yml | 6 +- docs/usage.md | 8 +++ lib/NfcoreTemplate.groovy | 55 +++++++++++++++ lib/Utils.groovy | 21 ++++-- lib/WorkflowChipseq.groovy | 19 ++++++ main.nf | 3 +- modules.json | 27 ++++---- .../custom/dumpsoftwareversions/main.nf | 8 +-- .../custom/dumpsoftwareversions/meta.yml | 0 .../templates/dumpsoftwareversions.py | 0 modules/nf-core/{modules => }/fastqc/main.nf | 12 ++++ modules/nf-core/{modules => }/fastqc/meta.yml | 0 modules/nf-core/modules/multiqc/main.nf | 31 --------- modules/nf-core/multiqc/main.nf | 53 +++++++++++++++ .../nf-core/{modules => }/multiqc/meta.yml | 15 +++++ nextflow.config | 7 +- nextflow_schema.json | 18 +++++ workflows/chipseq.nf | 26 ++++--- 26 files changed, 348 insertions(+), 74 deletions(-) create mode 100644 assets/adaptivecard.json create mode 100644 assets/methods_description_template.yml mode change 100755 => 100644 lib/Utils.groovy rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/main.nf (79%) rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/meta.yml (100%) rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py (100%) rename modules/nf-core/{modules => }/fastqc/main.nf (85%) rename modules/nf-core/{modules => }/fastqc/meta.yml (100%) delete mode 100644 modules/nf-core/modules/multiqc/main.nf create mode 100644 modules/nf-core/multiqc/main.nf rename modules/nf-core/{modules => }/multiqc/meta.yml (73%) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index c6f0c9c6..d54a33a5 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -28,3 +28,7 @@ jobs: "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/chipseq/results-${{ github.sha }}" } profiles: test_full,aws_tower + - uses: actions/upload-artifact@v3 + with: + name: Tower debug log file + path: tower_action_*.log diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 73640cc6..ecd16998 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -23,3 +23,7 @@ jobs: "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/chipseq/results-test-${{ github.sha }}" } profiles: test,aws_tower + - uses: actions/upload-artifact@v3 + with: + name: Tower debug log file + path: tower_action_*.log diff --git a/.prettierignore b/.prettierignore index d0e7ae58..eb74a574 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,4 +1,5 @@ email_template.html +adaptivecard.json .nextflow* work/ data/ diff --git a/CITATION.cff b/CITATION.cff index 4533e2f2..017666c0 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -13,8 +13,8 @@ authors: given-names: Johannes - family-names: Wilm given-names: Andreas - - family-names: Ulysse Garcia - given-names: Maxime + - family-names: Garcia + given-names: Maxime Ulysse - family-names: Di Tommaso given-names: Paolo - family-names: Nahnsen @@ -39,8 +39,8 @@ prefered-citation: given-names: Johannes - family-names: Wilm given-names: Andreas - - family-names: Ulysse Garcia - given-names: Maxime + - family-names: Garcia + given-names: Maxime Ulysse - family-names: Di Tommaso given-names: Paolo - family-names: Nahnsen diff --git a/LICENSE b/LICENSE index e6309f57..9ddb87a2 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) Philip Ewels, Jose Espinosa-Carrasco, Harshil Patel +Copyright (c) Espinosa-Carrasco J, Patel H, Wang C, Ewels P Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index 095d129c..0b1f436d 100644 --- a/README.md +++ b/README.md @@ -62,7 +62,7 @@ The nf-core/chipseq pipeline comes with documentation about the pipeline [usage] ## Credits -nf-core/chipseq was originally written by Philip Ewels, Jose Espinosa-Carrasco, Harshil Patel. +nf-core/chipseq was originally written by Espinosa-Carrasco J, Patel H, Wang C, Ewels P. We thank the following people for their extensive assistance in the development of this pipeline: diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json new file mode 100644 index 00000000..8c6fb5a7 --- /dev/null +++ b/assets/adaptivecard.json @@ -0,0 +1,67 @@ +{ + "type": "message", + "attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/chipseq v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml new file mode 100644 index 00000000..a30b1eb6 --- /dev/null +++ b/assets/methods_description_template.yml @@ -0,0 +1,25 @@ +id: "nf-core-chipseq-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/chipseq Methods Description" +section_href: "https://github.com/nf-core/chipseq" +plot_type: "html" +## TODO nf-core: Update the HTML below to your prefered methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

Methods

+

Data was processed using nf-core/chipseq v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020).

+

The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

+ 
${workflow.commandLine}
+

References

+ +
+
Notes:
+ +
diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 5b7d1652..46d7ec2d 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -3,9 +3,11 @@ report_comment: > analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: - software_versions: + "nf-core-chipseq-methods-description": order: -1000 - "nf-core-chipseq-summary": + software_versions: order: -1001 + "nf-core-chipseq-summary": + order: -1002 export_plots: true diff --git a/docs/usage.md b/docs/usage.md index 997dfd2c..776325ae 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -237,6 +237,14 @@ See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). +## Azure Resource Requests + +To be used with the `azurebatch` profile by specifying the `-profile azurebatch`. +We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required. + +Note that the choice of VM size depends on your quota and the overall workload during the analysis. +For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes). + ## Running in the background Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 2fc0a9b9..27feb009 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -145,6 +145,61 @@ class NfcoreTemplate { output_tf.withWriter { w -> w << email_txt } } + // + // Construct and send adaptive card + // https://adaptivecards.io + // + public static void adaptivecard(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = workflow.manifest.version + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + def hf = new File("$projectDir/assets/adaptivecard.json") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + // // Print pipeline summary on completion // diff --git a/lib/Utils.groovy b/lib/Utils.groovy old mode 100755 new mode 100644 index 28567bd7..8d030f4e --- a/lib/Utils.groovy +++ b/lib/Utils.groovy @@ -21,19 +21,26 @@ class Utils { } // Check that all channels are present - def required_channels = ['conda-forge', 'bioconda', 'defaults'] - def conda_check_failed = !required_channels.every { ch -> ch in channels } + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order - conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) - conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } - if (conda_check_failed) { + if (channels_missing | channel_priority_violation) { log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + " There is a problem with your Conda configuration!\n\n" + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + - " NB: The order of the channels matters!\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" } } diff --git a/lib/WorkflowChipseq.groovy b/lib/WorkflowChipseq.groovy index a5952af0..b85fcd58 100755 --- a/lib/WorkflowChipseq.groovy +++ b/lib/WorkflowChipseq.groovy @@ -2,6 +2,8 @@ // This file holds several functions specific to the workflow/chipseq.nf in the nf-core/chipseq pipeline // +import groovy.text.SimpleTemplateEngine + class WorkflowChipseq { // @@ -42,6 +44,23 @@ class WorkflowChipseq { yaml_file_text += "data: |\n" yaml_file_text += "${summary_section}" return yaml_file_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html }// // Exit pipeline if incorrect --genome key provided // diff --git a/main.nf b/main.nf index d6adc851..06d6e411 100644 --- a/main.nf +++ b/main.nf @@ -4,7 +4,8 @@ nf-core/chipseq ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Github : https://github.com/nf-core/chipseq -Website: https://nf-co.re/chipseq + + Website: https://nf-co.re/chipseq Slack : https://nfcore.slack.com/channels/chipseq ---------------------------------------------------------------------------------------- */ diff --git a/modules.json b/modules.json index db838714..cfa6d32f 100644 --- a/modules.json +++ b/modules.json @@ -2,20 +2,21 @@ "name": "nf-core/chipseq", "homePage": "https://github.com/nf-core/chipseq", "repos": { - "nf-core/modules": { - "git_url": "https://github.com/nf-core/modules.git", + "https://github.com/nf-core/modules.git": { "modules": { - "custom/dumpsoftwareversions": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d", - "branch": "master" - }, - "fastqc": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d", - "branch": "master" - }, - "multiqc": { - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d", - "branch": "master" + "nf-core": { + "custom/dumpsoftwareversions": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "fastqc": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "multiqc": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + } } } } diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf similarity index 79% rename from modules/nf-core/modules/custom/dumpsoftwareversions/main.nf rename to modules/nf-core/custom/dumpsoftwareversions/main.nf index 327d5100..cebb6e05 100644 --- a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -1,11 +1,11 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_low' + label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda (params.enable_conda ? "bioconda::multiqc=1.11" : null) + conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.11--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.11--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml similarity index 100% rename from modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml rename to modules/nf-core/custom/dumpsoftwareversions/meta.yml diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py similarity index 100% rename from modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py rename to modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/fastqc/main.nf similarity index 85% rename from modules/nf-core/modules/fastqc/main.nf rename to modules/nf-core/fastqc/main.nf index ed6b8c50..05730368 100644 --- a/modules/nf-core/modules/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -44,4 +44,16 @@ process FASTQC { END_VERSIONS """ } + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.html + touch ${prefix}.zip + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + END_VERSIONS + """ } diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml similarity index 100% rename from modules/nf-core/modules/fastqc/meta.yml rename to modules/nf-core/fastqc/meta.yml diff --git a/modules/nf-core/modules/multiqc/main.nf b/modules/nf-core/modules/multiqc/main.nf deleted file mode 100644 index 1264aac1..00000000 --- a/modules/nf-core/modules/multiqc/main.nf +++ /dev/null @@ -1,31 +0,0 @@ -process MULTIQC { - label 'process_medium' - - conda (params.enable_conda ? 'bioconda::multiqc=1.12' : null) - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.12--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.12--pyhdfd78af_0' }" - - input: - path multiqc_files - - output: - path "*multiqc_report.html", emit: report - path "*_data" , emit: data - path "*_plots" , optional:true, emit: plots - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - """ - multiqc -f $args . - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) - END_VERSIONS - """ -} diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf new file mode 100644 index 00000000..a8159a57 --- /dev/null +++ b/modules/nf-core/multiqc/main.nf @@ -0,0 +1,53 @@ +process MULTIQC { + label 'process_single' + + conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + + input: + path multiqc_files, stageAs: "?/*" + path(multiqc_config) + path(extra_multiqc_config) + path(multiqc_logo) + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def config = multiqc_config ? "--config $multiqc_config" : '' + def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + """ + multiqc \\ + --force \\ + $args \\ + $config \\ + $extra_config \\ + . + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ + + stub: + """ + touch multiqc_data + touch multiqc_plots + touch multiqc_report.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ +} diff --git a/modules/nf-core/modules/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml similarity index 73% rename from modules/nf-core/modules/multiqc/meta.yml rename to modules/nf-core/multiqc/meta.yml index 6fa891ef..ebc29b27 100644 --- a/modules/nf-core/modules/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -12,11 +12,25 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] + input: - multiqc_files: type: file description: | List of reports / files recognised by MultiQC, for example the html and zip output of FastQC + - multiqc_config: + type: file + description: Optional config yml for MultiQC + pattern: "*.{yml,yaml}" + - extra_multiqc_config: + type: file + description: Second optional config yml for MultiQC. Will override common sections in multiqc_config. + pattern: "*.{yml,yaml}" + - multiqc_logo: + type: file + description: Optional logo file for MultiQC + pattern: "*.{png}" + output: - report: type: file @@ -38,3 +52,4 @@ authors: - "@abhi18av" - "@bunop" - "@drpatelh" + - "@jfy133" diff --git a/nextflow.config b/nextflow.config index 08da317e..fa8bfa21 100644 --- a/nextflow.config +++ b/nextflow.config @@ -21,7 +21,9 @@ params { // MultiQC options multiqc_config = null multiqc_title = null + multiqc_logo = null max_multiqc_email_size = '25.MB' + multiqc_methods_description = null // Boilerplate options outdir = null @@ -31,6 +33,7 @@ params { email_on_fail = null plaintext_email = false monochrome_logs = false + hook_url = null help = false validate_params = true show_hidden_params = false @@ -74,7 +77,6 @@ try { // } - profiles { debug { process.beforeScript = 'echo $HOSTNAME' } conda { @@ -183,12 +185,13 @@ dag { manifest { name = 'nf-core/chipseq' - author = 'Philip Ewels, Jose Espinosa-Carrasco, Harshil Patel' + author = 'Espinosa-Carrasco J, Patel H, Wang C, Ewels P' homePage = 'https://github.com/nf-core/chipseq' description = 'ChIP-seq peak-calling and differential analysis pipeline.' mainScript = 'main.nf' nextflowVersion = '!>=21.10.3' version = '2.0.0' + doi = '' } // Load modules.config for DSL2 module specific options diff --git a/nextflow_schema.json b/nextflow_schema.json index 8080b723..dd7c26cf 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -213,12 +213,30 @@ "fa_icon": "fas fa-palette", "hidden": true }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", + "hidden": true + }, "multiqc_config": { "type": "string", "description": "Custom config file to supply to MultiQC.", "fa_icon": "fas fa-cog", "hidden": true }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, "tracedir": { "type": "string", "description": "Directory to keep pipeline Nextflow logs and reports.", diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index ea403618..a39a34d2 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -23,8 +23,10 @@ if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input sample ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() +ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() +ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() +ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -46,9 +48,9 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' // // MODULE: Installed directly from nf-core/modules // -include { FASTQC } from '../modules/nf-core/modules/fastqc/main' -include { MULTIQC } from '../modules/nf-core/modules/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -89,15 +91,20 @@ workflow CHIPSEQ { workflow_summary = WorkflowChipseq.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) + methods_description = WorkflowChipseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description) + ch_methods_description = Channel.value(methods_description) + ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(Channel.from(ch_multiqc_config)) - ch_multiqc_files = ch_multiqc_files.mix(ch_multiqc_custom_config.collect().ifEmpty([])) ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) MULTIQC ( - ch_multiqc_files.collect() + ch_multiqc_files.collect(), + ch_multiqc_config.collect().ifEmpty([]), + ch_multiqc_custom_config.collect().ifEmpty([]), + ch_multiqc_logo.collect().ifEmpty([]) ) multiqc_report = MULTIQC.out.report.toList() ch_versions = ch_versions.mix(MULTIQC.out.versions) @@ -114,6 +121,9 @@ workflow.onComplete { NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) } NfcoreTemplate.summary(workflow, params, log) + if (params.hook_url) { + NfcoreTemplate.adaptivecard(workflow, params, summary_params, projectDir, log) + } } /* From f8b2f3c013c78c812b13f211eebfe18474ce221f Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 Oct 2022 15:03:11 +0200 Subject: [PATCH 002/201] Bump pipeline version to 2.1.0dev --- CHANGELOG.md | 4 ++++ nextflow.config | 2 +- 2 files changed, 5 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ea9b4606..de7b178f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,10 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## [Unpublished Version / DEV] + +### Enhancements & fixes + ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 ### Enhancements & fixes diff --git a/nextflow.config b/nextflow.config index e7c2b71d..da798582 100644 --- a/nextflow.config +++ b/nextflow.config @@ -232,7 +232,7 @@ manifest { description = 'ChIP-seq peak-calling and differential analysis pipeline.' mainScript = 'main.nf' nextflowVersion = '!>=21.10.3' - version = '2.0.0' + version = '2.1.0dev' } // Load modules.config for DSL2 module specific options From 688be0a143c454e0d18e95d826acaf394f5b3dfa Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 Oct 2022 15:07:16 +0200 Subject: [PATCH 003/201] Run nf-core modules update --- modules.json | 259 +++++++++--------- .../{modules => }/bowtie2/align/main.nf | 0 .../{modules => }/bowtie2/align/meta.yml | 0 .../{modules => }/bowtie2/build/main.nf | 0 .../{modules => }/bowtie2/build/meta.yml | 0 .../nf-core/{modules => }/bwa/index/main.nf | 0 .../nf-core/{modules => }/bwa/index/meta.yml | 0 modules/nf-core/{modules => }/bwa/mem/main.nf | 0 .../nf-core/{modules => }/bwa/mem/meta.yml | 0 .../{modules => }/chromap/chromap/main.nf | 0 .../{modules => }/chromap/chromap/meta.yml | 0 .../{modules => }/chromap/index/main.nf | 0 .../{modules => }/chromap/index/meta.yml | 0 .../custom/dumpsoftwareversions/main.nf | 0 .../custom/dumpsoftwareversions/meta.yml | 0 .../templates/dumpsoftwareversions.py | 0 .../custom/getchromsizes/main.nf | 0 .../custom/getchromsizes/meta.yml | 0 .../deeptools/computematrix/main.nf | 0 .../deeptools/computematrix/meta.yml | 0 .../deeptools/plotfingerprint/main.nf | 0 .../deeptools/plotfingerprint/meta.yml | 0 .../deeptools/plotheatmap/main.nf | 0 .../deeptools/plotheatmap/meta.yml | 0 .../deeptools/plotprofile/main.nf | 0 .../deeptools/plotprofile/meta.yml | 0 modules/nf-core/{modules => }/fastqc/main.nf | 0 modules/nf-core/{modules => }/fastqc/meta.yml | 0 modules/nf-core/{modules => }/gffread/main.nf | 0 .../nf-core/{modules => }/gffread/meta.yml | 0 modules/nf-core/{modules => }/gunzip/main.nf | 0 modules/nf-core/{modules => }/gunzip/meta.yml | 0 .../{modules => }/homer/annotatepeaks/main.nf | 0 .../homer/annotatepeaks/meta.yml | 0 .../{modules => }/khmer/uniquekmers/main.nf | 0 .../{modules => }/khmer/uniquekmers/meta.yml | 0 .../{modules => }/macs2/callpeak/main.nf | 0 .../{modules => }/macs2/callpeak/meta.yml | 0 .../phantompeakqualtools/main.nf | 0 .../phantompeakqualtools/meta.yml | 0 .../picard/collectmultiplemetrics/main.nf | 0 .../picard/collectmultiplemetrics/meta.yml | 0 .../picard/markduplicates/main.nf | 0 .../picard/markduplicates/meta.yml | 0 .../picard/mergesamfiles/main.nf | 0 .../picard/mergesamfiles/meta.yml | 0 .../{modules => }/preseq/lcextrap/main.nf | 0 .../{modules => }/preseq/lcextrap/meta.yml | 0 .../{modules => }/samtools/flagstat/main.nf | 0 .../{modules => }/samtools/flagstat/meta.yml | 0 .../{modules => }/samtools/idxstats/main.nf | 0 .../{modules => }/samtools/idxstats/meta.yml | 0 .../{modules => }/samtools/index/main.nf | 0 .../{modules => }/samtools/index/meta.yml | 0 .../{modules => }/samtools/sort/main.nf | 0 .../{modules => }/samtools/sort/meta.yml | 0 .../{modules => }/samtools/stats/main.nf | 0 .../{modules => }/samtools/stats/meta.yml | 0 .../subread/featurecounts/main.nf | 0 .../subread/featurecounts/meta.yml | 0 .../nf-core/{modules => }/trimgalore/main.nf | 0 .../nf-core/{modules => }/trimgalore/meta.yml | 0 .../ucsc/bedgraphtobigwig/main.nf | 0 .../ucsc/bedgraphtobigwig/meta.yml | 0 modules/nf-core/{modules => }/untar/main.nf | 0 modules/nf-core/{modules => }/untar/meta.yml | 0 66 files changed, 130 insertions(+), 129 deletions(-) rename modules/nf-core/{modules => }/bowtie2/align/main.nf (100%) rename modules/nf-core/{modules => }/bowtie2/align/meta.yml (100%) rename modules/nf-core/{modules => }/bowtie2/build/main.nf (100%) rename modules/nf-core/{modules => }/bowtie2/build/meta.yml (100%) rename modules/nf-core/{modules => }/bwa/index/main.nf (100%) rename modules/nf-core/{modules => }/bwa/index/meta.yml (100%) rename modules/nf-core/{modules => }/bwa/mem/main.nf (100%) rename modules/nf-core/{modules => }/bwa/mem/meta.yml (100%) rename modules/nf-core/{modules => }/chromap/chromap/main.nf (100%) rename modules/nf-core/{modules => }/chromap/chromap/meta.yml (100%) rename modules/nf-core/{modules => }/chromap/index/main.nf (100%) rename modules/nf-core/{modules => }/chromap/index/meta.yml (100%) rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/main.nf (100%) rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/meta.yml (100%) rename modules/nf-core/{modules => }/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py (100%) rename modules/nf-core/{modules => }/custom/getchromsizes/main.nf (100%) rename modules/nf-core/{modules => }/custom/getchromsizes/meta.yml (100%) rename modules/nf-core/{modules => }/deeptools/computematrix/main.nf (100%) rename modules/nf-core/{modules => }/deeptools/computematrix/meta.yml (100%) rename modules/nf-core/{modules => }/deeptools/plotfingerprint/main.nf (100%) rename modules/nf-core/{modules => }/deeptools/plotfingerprint/meta.yml (100%) rename modules/nf-core/{modules => }/deeptools/plotheatmap/main.nf (100%) rename modules/nf-core/{modules => }/deeptools/plotheatmap/meta.yml (100%) rename modules/nf-core/{modules => }/deeptools/plotprofile/main.nf (100%) rename modules/nf-core/{modules => }/deeptools/plotprofile/meta.yml (100%) rename modules/nf-core/{modules => }/fastqc/main.nf (100%) rename modules/nf-core/{modules => }/fastqc/meta.yml (100%) rename modules/nf-core/{modules => }/gffread/main.nf (100%) rename modules/nf-core/{modules => }/gffread/meta.yml (100%) rename modules/nf-core/{modules => }/gunzip/main.nf (100%) rename modules/nf-core/{modules => }/gunzip/meta.yml (100%) rename modules/nf-core/{modules => }/homer/annotatepeaks/main.nf (100%) rename modules/nf-core/{modules => }/homer/annotatepeaks/meta.yml (100%) rename modules/nf-core/{modules => }/khmer/uniquekmers/main.nf (100%) rename modules/nf-core/{modules => }/khmer/uniquekmers/meta.yml (100%) rename modules/nf-core/{modules => }/macs2/callpeak/main.nf (100%) rename modules/nf-core/{modules => }/macs2/callpeak/meta.yml (100%) rename modules/nf-core/{modules => }/phantompeakqualtools/main.nf (100%) rename modules/nf-core/{modules => }/phantompeakqualtools/meta.yml (100%) rename modules/nf-core/{modules => }/picard/collectmultiplemetrics/main.nf (100%) rename modules/nf-core/{modules => }/picard/collectmultiplemetrics/meta.yml (100%) rename modules/nf-core/{modules => }/picard/markduplicates/main.nf (100%) rename modules/nf-core/{modules => }/picard/markduplicates/meta.yml (100%) rename modules/nf-core/{modules => }/picard/mergesamfiles/main.nf (100%) rename modules/nf-core/{modules => }/picard/mergesamfiles/meta.yml (100%) rename modules/nf-core/{modules => }/preseq/lcextrap/main.nf (100%) rename modules/nf-core/{modules => }/preseq/lcextrap/meta.yml (100%) rename modules/nf-core/{modules => }/samtools/flagstat/main.nf (100%) rename modules/nf-core/{modules => }/samtools/flagstat/meta.yml (100%) rename modules/nf-core/{modules => }/samtools/idxstats/main.nf (100%) rename modules/nf-core/{modules => }/samtools/idxstats/meta.yml (100%) rename modules/nf-core/{modules => }/samtools/index/main.nf (100%) rename modules/nf-core/{modules => }/samtools/index/meta.yml (100%) rename modules/nf-core/{modules => }/samtools/sort/main.nf (100%) rename modules/nf-core/{modules => }/samtools/sort/meta.yml (100%) rename modules/nf-core/{modules => }/samtools/stats/main.nf (100%) rename modules/nf-core/{modules => }/samtools/stats/meta.yml (100%) rename modules/nf-core/{modules => }/subread/featurecounts/main.nf (100%) rename modules/nf-core/{modules => }/subread/featurecounts/meta.yml (100%) rename modules/nf-core/{modules => }/trimgalore/main.nf (100%) rename modules/nf-core/{modules => }/trimgalore/meta.yml (100%) rename modules/nf-core/{modules => }/ucsc/bedgraphtobigwig/main.nf (100%) rename modules/nf-core/{modules => }/ucsc/bedgraphtobigwig/meta.yml (100%) rename modules/nf-core/{modules => }/untar/main.nf (100%) rename modules/nf-core/{modules => }/untar/meta.yml (100%) diff --git a/modules.json b/modules.json index 81c94b44..d9f0b83d 100644 --- a/modules.json +++ b/modules.json @@ -2,136 +2,137 @@ "name": "nf-core/chipseq", "homePage": "https://github.com/nf-core/chipseq", "repos": { - "nf-core/modules": { - "git_url": "https://github.com/nf-core/modules.git", + "https://github.com/nf-core/modules.git": { "modules": { - "bowtie2/align": { - "branch": "master", - "git_sha": "848ee9a215d02d80be033bfa60881700f2bd914c" - }, - "bowtie2/build": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "bwa/index": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "bwa/mem": { - "branch": "master", - "git_sha": "4f5274c3de0c9521f5033893ff61057a74c45ba9" - }, - "chromap/chromap": { - "branch": "master", - "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" - }, - "chromap/index": { - "branch": "master", - "git_sha": "dbb46c9b635080b132bab4b8d5b9a14f0d1c22e7" - }, - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "e5b44499efcf6f7fb24874886bac60591c5d94dd" - }, - "custom/getchromsizes": { - "branch": "master", - "git_sha": "213403187932dbbdd936a04474cc8cd8abae7a08" - }, - "deeptools/computematrix": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "deeptools/plotfingerprint": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "deeptools/plotheatmap": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "deeptools/plotprofile": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "fastqc": { - "branch": "master", - "git_sha": "49b18b1639f4f7104187058866a8fab33332bdfe" - }, - "gffread": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "gunzip": { - "branch": "master", - "git_sha": "fa37e0662690c4ec4260dae282fbce08777503e6" - }, - "homer/annotatepeaks": { - "branch": "master", - "git_sha": "233fa70811a03a4cecb2ece483b5c8396e2cee1d" - }, - "khmer/uniquekmers": { - "branch": "master", - "git_sha": "82fdff4fb4ce6cafcc028a7503da835427f35352" - }, - "macs2/callpeak": { - "branch": "master", - "git_sha": "f0800157544a82ae222931764483331a81812012" - }, - "phantompeakqualtools": { - "branch": "master", - "git_sha": "233fa70811a03a4cecb2ece483b5c8396e2cee1d" - }, - "picard/collectmultiplemetrics": { - "branch": "master", - "git_sha": "682f789f93070bd047868300dd018faf3d434e7c" - }, - "picard/markduplicates": { - "branch": "master", - "git_sha": "682f789f93070bd047868300dd018faf3d434e7c" - }, - "picard/mergesamfiles": { - "branch": "master", - "git_sha": "682f789f93070bd047868300dd018faf3d434e7c" - }, - "preseq/lcextrap": { - "branch": "master", - "git_sha": "7111e571cc5b6069de4673cd6165af680f17b4d7" - }, - "samtools/flagstat": { - "branch": "master", - "git_sha": "ecece498f10b47b7c9d06f53a310cea5811b4c5f" - }, - "samtools/idxstats": { - "branch": "master", - "git_sha": "ecece498f10b47b7c9d06f53a310cea5811b4c5f" - }, - "samtools/index": { - "branch": "master", - "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" - }, - "samtools/sort": { - "branch": "master", - "git_sha": "897c33d5da084b61109500ee44c01da2d3e4e773" - }, - "samtools/stats": { - "branch": "master", - "git_sha": "f48a24770e24358e58de66e9b805a70d77cd154b" - }, - "subread/featurecounts": { - "branch": "master", - "git_sha": "e745e167c1020928ef20ea1397b6b4d230681b4d" - }, - "trimgalore": { - "branch": "master", - "git_sha": "85ec13ff1fc2196c5a507ea497de468101baabed" - }, - "ucsc/bedgraphtobigwig": { - "branch": "master", - "git_sha": "233fa70811a03a4cecb2ece483b5c8396e2cee1d" - }, - "untar": { - "branch": "master", - "git_sha": "51be617b1ca9bff973655eb899d591ed6ab253b5" + "nf-core": { + "bowtie2/align": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "bowtie2/build": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "bwa/index": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "bwa/mem": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "chromap/chromap": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "chromap/index": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "custom/dumpsoftwareversions": { + "branch": "master", + "git_sha": "8022c68e7403eecbd8ba9c49496f69f8c49d50f0" + }, + "custom/getchromsizes": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "deeptools/computematrix": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "deeptools/plotfingerprint": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "deeptools/plotheatmap": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "deeptools/plotprofile": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "fastqc": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "gffread": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "gunzip": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "homer/annotatepeaks": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "khmer/uniquekmers": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "macs2/callpeak": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "phantompeakqualtools": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "picard/collectmultiplemetrics": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "picard/markduplicates": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "picard/mergesamfiles": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "preseq/lcextrap": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "samtools/flagstat": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "samtools/idxstats": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "samtools/index": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "samtools/sort": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "samtools/stats": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "subread/featurecounts": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "trimgalore": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "ucsc/bedgraphtobigwig": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + }, + "untar": { + "branch": "master", + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + } } } } diff --git a/modules/nf-core/modules/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf similarity index 100% rename from modules/nf-core/modules/bowtie2/align/main.nf rename to modules/nf-core/bowtie2/align/main.nf diff --git a/modules/nf-core/modules/bowtie2/align/meta.yml b/modules/nf-core/bowtie2/align/meta.yml similarity index 100% rename from modules/nf-core/modules/bowtie2/align/meta.yml rename to modules/nf-core/bowtie2/align/meta.yml diff --git a/modules/nf-core/modules/bowtie2/build/main.nf b/modules/nf-core/bowtie2/build/main.nf similarity index 100% rename from modules/nf-core/modules/bowtie2/build/main.nf rename to modules/nf-core/bowtie2/build/main.nf diff --git a/modules/nf-core/modules/bowtie2/build/meta.yml b/modules/nf-core/bowtie2/build/meta.yml similarity index 100% rename from modules/nf-core/modules/bowtie2/build/meta.yml rename to modules/nf-core/bowtie2/build/meta.yml diff --git a/modules/nf-core/modules/bwa/index/main.nf b/modules/nf-core/bwa/index/main.nf similarity index 100% rename from modules/nf-core/modules/bwa/index/main.nf rename to modules/nf-core/bwa/index/main.nf diff --git a/modules/nf-core/modules/bwa/index/meta.yml b/modules/nf-core/bwa/index/meta.yml similarity index 100% rename from modules/nf-core/modules/bwa/index/meta.yml rename to modules/nf-core/bwa/index/meta.yml diff --git a/modules/nf-core/modules/bwa/mem/main.nf b/modules/nf-core/bwa/mem/main.nf similarity index 100% rename from modules/nf-core/modules/bwa/mem/main.nf rename to modules/nf-core/bwa/mem/main.nf diff --git a/modules/nf-core/modules/bwa/mem/meta.yml b/modules/nf-core/bwa/mem/meta.yml similarity index 100% rename from modules/nf-core/modules/bwa/mem/meta.yml rename to modules/nf-core/bwa/mem/meta.yml diff --git a/modules/nf-core/modules/chromap/chromap/main.nf b/modules/nf-core/chromap/chromap/main.nf similarity index 100% rename from modules/nf-core/modules/chromap/chromap/main.nf rename to modules/nf-core/chromap/chromap/main.nf diff --git a/modules/nf-core/modules/chromap/chromap/meta.yml b/modules/nf-core/chromap/chromap/meta.yml similarity index 100% rename from modules/nf-core/modules/chromap/chromap/meta.yml rename to modules/nf-core/chromap/chromap/meta.yml diff --git a/modules/nf-core/modules/chromap/index/main.nf b/modules/nf-core/chromap/index/main.nf similarity index 100% rename from modules/nf-core/modules/chromap/index/main.nf rename to modules/nf-core/chromap/index/main.nf diff --git a/modules/nf-core/modules/chromap/index/meta.yml b/modules/nf-core/chromap/index/meta.yml similarity index 100% rename from modules/nf-core/modules/chromap/index/meta.yml rename to modules/nf-core/chromap/index/meta.yml diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf similarity index 100% rename from modules/nf-core/modules/custom/dumpsoftwareversions/main.nf rename to modules/nf-core/custom/dumpsoftwareversions/main.nf diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml similarity index 100% rename from modules/nf-core/modules/custom/dumpsoftwareversions/meta.yml rename to modules/nf-core/custom/dumpsoftwareversions/meta.yml diff --git a/modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py similarity index 100% rename from modules/nf-core/modules/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py rename to modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules/nf-core/modules/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf similarity index 100% rename from modules/nf-core/modules/custom/getchromsizes/main.nf rename to modules/nf-core/custom/getchromsizes/main.nf diff --git a/modules/nf-core/modules/custom/getchromsizes/meta.yml b/modules/nf-core/custom/getchromsizes/meta.yml similarity index 100% rename from modules/nf-core/modules/custom/getchromsizes/meta.yml rename to modules/nf-core/custom/getchromsizes/meta.yml diff --git a/modules/nf-core/modules/deeptools/computematrix/main.nf b/modules/nf-core/deeptools/computematrix/main.nf similarity index 100% rename from modules/nf-core/modules/deeptools/computematrix/main.nf rename to modules/nf-core/deeptools/computematrix/main.nf diff --git a/modules/nf-core/modules/deeptools/computematrix/meta.yml b/modules/nf-core/deeptools/computematrix/meta.yml similarity index 100% rename from modules/nf-core/modules/deeptools/computematrix/meta.yml rename to modules/nf-core/deeptools/computematrix/meta.yml diff --git a/modules/nf-core/modules/deeptools/plotfingerprint/main.nf b/modules/nf-core/deeptools/plotfingerprint/main.nf similarity index 100% rename from modules/nf-core/modules/deeptools/plotfingerprint/main.nf rename to modules/nf-core/deeptools/plotfingerprint/main.nf diff --git a/modules/nf-core/modules/deeptools/plotfingerprint/meta.yml b/modules/nf-core/deeptools/plotfingerprint/meta.yml similarity index 100% rename from modules/nf-core/modules/deeptools/plotfingerprint/meta.yml rename to modules/nf-core/deeptools/plotfingerprint/meta.yml diff --git a/modules/nf-core/modules/deeptools/plotheatmap/main.nf b/modules/nf-core/deeptools/plotheatmap/main.nf similarity index 100% rename from modules/nf-core/modules/deeptools/plotheatmap/main.nf rename to modules/nf-core/deeptools/plotheatmap/main.nf diff --git a/modules/nf-core/modules/deeptools/plotheatmap/meta.yml b/modules/nf-core/deeptools/plotheatmap/meta.yml similarity index 100% rename from modules/nf-core/modules/deeptools/plotheatmap/meta.yml rename to modules/nf-core/deeptools/plotheatmap/meta.yml diff --git a/modules/nf-core/modules/deeptools/plotprofile/main.nf b/modules/nf-core/deeptools/plotprofile/main.nf similarity index 100% rename from modules/nf-core/modules/deeptools/plotprofile/main.nf rename to modules/nf-core/deeptools/plotprofile/main.nf diff --git a/modules/nf-core/modules/deeptools/plotprofile/meta.yml b/modules/nf-core/deeptools/plotprofile/meta.yml similarity index 100% rename from modules/nf-core/modules/deeptools/plotprofile/meta.yml rename to modules/nf-core/deeptools/plotprofile/meta.yml diff --git a/modules/nf-core/modules/fastqc/main.nf b/modules/nf-core/fastqc/main.nf similarity index 100% rename from modules/nf-core/modules/fastqc/main.nf rename to modules/nf-core/fastqc/main.nf diff --git a/modules/nf-core/modules/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml similarity index 100% rename from modules/nf-core/modules/fastqc/meta.yml rename to modules/nf-core/fastqc/meta.yml diff --git a/modules/nf-core/modules/gffread/main.nf b/modules/nf-core/gffread/main.nf similarity index 100% rename from modules/nf-core/modules/gffread/main.nf rename to modules/nf-core/gffread/main.nf diff --git a/modules/nf-core/modules/gffread/meta.yml b/modules/nf-core/gffread/meta.yml similarity index 100% rename from modules/nf-core/modules/gffread/meta.yml rename to modules/nf-core/gffread/meta.yml diff --git a/modules/nf-core/modules/gunzip/main.nf b/modules/nf-core/gunzip/main.nf similarity index 100% rename from modules/nf-core/modules/gunzip/main.nf rename to modules/nf-core/gunzip/main.nf diff --git a/modules/nf-core/modules/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml similarity index 100% rename from modules/nf-core/modules/gunzip/meta.yml rename to modules/nf-core/gunzip/meta.yml diff --git a/modules/nf-core/modules/homer/annotatepeaks/main.nf b/modules/nf-core/homer/annotatepeaks/main.nf similarity index 100% rename from modules/nf-core/modules/homer/annotatepeaks/main.nf rename to modules/nf-core/homer/annotatepeaks/main.nf diff --git a/modules/nf-core/modules/homer/annotatepeaks/meta.yml b/modules/nf-core/homer/annotatepeaks/meta.yml similarity index 100% rename from modules/nf-core/modules/homer/annotatepeaks/meta.yml rename to modules/nf-core/homer/annotatepeaks/meta.yml diff --git a/modules/nf-core/modules/khmer/uniquekmers/main.nf b/modules/nf-core/khmer/uniquekmers/main.nf similarity index 100% rename from modules/nf-core/modules/khmer/uniquekmers/main.nf rename to modules/nf-core/khmer/uniquekmers/main.nf diff --git a/modules/nf-core/modules/khmer/uniquekmers/meta.yml b/modules/nf-core/khmer/uniquekmers/meta.yml similarity index 100% rename from modules/nf-core/modules/khmer/uniquekmers/meta.yml rename to modules/nf-core/khmer/uniquekmers/meta.yml diff --git a/modules/nf-core/modules/macs2/callpeak/main.nf b/modules/nf-core/macs2/callpeak/main.nf similarity index 100% rename from modules/nf-core/modules/macs2/callpeak/main.nf rename to modules/nf-core/macs2/callpeak/main.nf diff --git a/modules/nf-core/modules/macs2/callpeak/meta.yml b/modules/nf-core/macs2/callpeak/meta.yml similarity index 100% rename from modules/nf-core/modules/macs2/callpeak/meta.yml rename to modules/nf-core/macs2/callpeak/meta.yml diff --git a/modules/nf-core/modules/phantompeakqualtools/main.nf b/modules/nf-core/phantompeakqualtools/main.nf similarity index 100% rename from modules/nf-core/modules/phantompeakqualtools/main.nf rename to modules/nf-core/phantompeakqualtools/main.nf diff --git a/modules/nf-core/modules/phantompeakqualtools/meta.yml b/modules/nf-core/phantompeakqualtools/meta.yml similarity index 100% rename from modules/nf-core/modules/phantompeakqualtools/meta.yml rename to modules/nf-core/phantompeakqualtools/meta.yml diff --git a/modules/nf-core/modules/picard/collectmultiplemetrics/main.nf b/modules/nf-core/picard/collectmultiplemetrics/main.nf similarity index 100% rename from modules/nf-core/modules/picard/collectmultiplemetrics/main.nf rename to modules/nf-core/picard/collectmultiplemetrics/main.nf diff --git a/modules/nf-core/modules/picard/collectmultiplemetrics/meta.yml b/modules/nf-core/picard/collectmultiplemetrics/meta.yml similarity index 100% rename from modules/nf-core/modules/picard/collectmultiplemetrics/meta.yml rename to modules/nf-core/picard/collectmultiplemetrics/meta.yml diff --git a/modules/nf-core/modules/picard/markduplicates/main.nf b/modules/nf-core/picard/markduplicates/main.nf similarity index 100% rename from modules/nf-core/modules/picard/markduplicates/main.nf rename to modules/nf-core/picard/markduplicates/main.nf diff --git a/modules/nf-core/modules/picard/markduplicates/meta.yml b/modules/nf-core/picard/markduplicates/meta.yml similarity index 100% rename from modules/nf-core/modules/picard/markduplicates/meta.yml rename to modules/nf-core/picard/markduplicates/meta.yml diff --git a/modules/nf-core/modules/picard/mergesamfiles/main.nf b/modules/nf-core/picard/mergesamfiles/main.nf similarity index 100% rename from modules/nf-core/modules/picard/mergesamfiles/main.nf rename to modules/nf-core/picard/mergesamfiles/main.nf diff --git a/modules/nf-core/modules/picard/mergesamfiles/meta.yml b/modules/nf-core/picard/mergesamfiles/meta.yml similarity index 100% rename from modules/nf-core/modules/picard/mergesamfiles/meta.yml rename to modules/nf-core/picard/mergesamfiles/meta.yml diff --git a/modules/nf-core/modules/preseq/lcextrap/main.nf b/modules/nf-core/preseq/lcextrap/main.nf similarity index 100% rename from modules/nf-core/modules/preseq/lcextrap/main.nf rename to modules/nf-core/preseq/lcextrap/main.nf diff --git a/modules/nf-core/modules/preseq/lcextrap/meta.yml b/modules/nf-core/preseq/lcextrap/meta.yml similarity index 100% rename from modules/nf-core/modules/preseq/lcextrap/meta.yml rename to modules/nf-core/preseq/lcextrap/meta.yml diff --git a/modules/nf-core/modules/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf similarity index 100% rename from modules/nf-core/modules/samtools/flagstat/main.nf rename to modules/nf-core/samtools/flagstat/main.nf diff --git a/modules/nf-core/modules/samtools/flagstat/meta.yml b/modules/nf-core/samtools/flagstat/meta.yml similarity index 100% rename from modules/nf-core/modules/samtools/flagstat/meta.yml rename to modules/nf-core/samtools/flagstat/meta.yml diff --git a/modules/nf-core/modules/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf similarity index 100% rename from modules/nf-core/modules/samtools/idxstats/main.nf rename to modules/nf-core/samtools/idxstats/main.nf diff --git a/modules/nf-core/modules/samtools/idxstats/meta.yml b/modules/nf-core/samtools/idxstats/meta.yml similarity index 100% rename from modules/nf-core/modules/samtools/idxstats/meta.yml rename to modules/nf-core/samtools/idxstats/meta.yml diff --git a/modules/nf-core/modules/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf similarity index 100% rename from modules/nf-core/modules/samtools/index/main.nf rename to modules/nf-core/samtools/index/main.nf diff --git a/modules/nf-core/modules/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml similarity index 100% rename from modules/nf-core/modules/samtools/index/meta.yml rename to modules/nf-core/samtools/index/meta.yml diff --git a/modules/nf-core/modules/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf similarity index 100% rename from modules/nf-core/modules/samtools/sort/main.nf rename to modules/nf-core/samtools/sort/main.nf diff --git a/modules/nf-core/modules/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml similarity index 100% rename from modules/nf-core/modules/samtools/sort/meta.yml rename to modules/nf-core/samtools/sort/meta.yml diff --git a/modules/nf-core/modules/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf similarity index 100% rename from modules/nf-core/modules/samtools/stats/main.nf rename to modules/nf-core/samtools/stats/main.nf diff --git a/modules/nf-core/modules/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml similarity index 100% rename from modules/nf-core/modules/samtools/stats/meta.yml rename to modules/nf-core/samtools/stats/meta.yml diff --git a/modules/nf-core/modules/subread/featurecounts/main.nf b/modules/nf-core/subread/featurecounts/main.nf similarity index 100% rename from modules/nf-core/modules/subread/featurecounts/main.nf rename to modules/nf-core/subread/featurecounts/main.nf diff --git a/modules/nf-core/modules/subread/featurecounts/meta.yml b/modules/nf-core/subread/featurecounts/meta.yml similarity index 100% rename from modules/nf-core/modules/subread/featurecounts/meta.yml rename to modules/nf-core/subread/featurecounts/meta.yml diff --git a/modules/nf-core/modules/trimgalore/main.nf b/modules/nf-core/trimgalore/main.nf similarity index 100% rename from modules/nf-core/modules/trimgalore/main.nf rename to modules/nf-core/trimgalore/main.nf diff --git a/modules/nf-core/modules/trimgalore/meta.yml b/modules/nf-core/trimgalore/meta.yml similarity index 100% rename from modules/nf-core/modules/trimgalore/meta.yml rename to modules/nf-core/trimgalore/meta.yml diff --git a/modules/nf-core/modules/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf similarity index 100% rename from modules/nf-core/modules/ucsc/bedgraphtobigwig/main.nf rename to modules/nf-core/ucsc/bedgraphtobigwig/main.nf diff --git a/modules/nf-core/modules/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml similarity index 100% rename from modules/nf-core/modules/ucsc/bedgraphtobigwig/meta.yml rename to modules/nf-core/ucsc/bedgraphtobigwig/meta.yml diff --git a/modules/nf-core/modules/untar/main.nf b/modules/nf-core/untar/main.nf similarity index 100% rename from modules/nf-core/modules/untar/main.nf rename to modules/nf-core/untar/main.nf diff --git a/modules/nf-core/modules/untar/meta.yml b/modules/nf-core/untar/meta.yml similarity index 100% rename from modules/nf-core/modules/untar/meta.yml rename to modules/nf-core/untar/meta.yml From 2ec84ef2c5a89cc6ae2decf45603c8a07e746399 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 Oct 2022 15:42:52 +0200 Subject: [PATCH 004/201] Re-install custom/getchromsizes module --- modules/nf-core/custom/getchromsizes/main.nf | 24 ++++++++++++++----- modules/nf-core/custom/getchromsizes/meta.yml | 19 +++++++++++++-- 2 files changed, 35 insertions(+), 8 deletions(-) diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf index 0eabf3a4..8e1693d4 100644 --- a/modules/nf-core/custom/getchromsizes/main.nf +++ b/modules/nf-core/custom/getchromsizes/main.nf @@ -1,6 +1,6 @@ process CUSTOM_GETCHROMSIZES { tag "$fasta" - label 'process_low' + label 'process_single' conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? @@ -8,12 +8,13 @@ process CUSTOM_GETCHROMSIZES { 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: - path fasta + tuple val(meta), path(fasta) output: - path '*.sizes' , emit: sizes - path '*.fai' , emit: fai - path "versions.yml", emit: versions + tuple val(meta), path ("*.sizes"), emit: sizes + tuple val(meta), path ("*.fai") , emit: fai + tuple val(meta), path ("*.gzi") , emit: gzi, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -26,7 +27,18 @@ process CUSTOM_GETCHROMSIZES { cat <<-END_VERSIONS > versions.yml "${task.process}": - custom: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ + + stub: + """ + touch ${fasta}.fai + touch ${fasta}.sizes + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + getchromsizes: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ } diff --git a/modules/nf-core/custom/getchromsizes/meta.yml b/modules/nf-core/custom/getchromsizes/meta.yml index ee6c2571..219ca1d8 100644 --- a/modules/nf-core/custom/getchromsizes/meta.yml +++ b/modules/nf-core/custom/getchromsizes/meta.yml @@ -14,12 +14,22 @@ tools: licence: ["MIT"] input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] - fasta: type: file description: FASTA file - pattern: "*.{fasta}" + pattern: "*.{fa,fasta,fna,fas}" output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] - sizes: type: file description: File containing chromosome lengths @@ -28,11 +38,16 @@ output: type: file description: FASTA index file pattern: "*.{fai}" + - gzi: + type: file + description: Optional gzip index file for compressed inputs + pattern: "*.gzi" - versions: type: file - description: File containing software version + description: File containing software versions pattern: "versions.yml" authors: - "@tamara-hodgetts" - "@chris-cheshire" + - "@muffato" From d69bd44f83c7fbacf129740ea5b59d26085badd6 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 Oct 2022 15:47:49 +0200 Subject: [PATCH 005/201] Patch custom/getchromsizes module --- modules.json | 3 ++- .../getchromsizes/custom-getchromsizes.diff | 23 +++++++++++++++++++ modules/nf-core/custom/getchromsizes/main.nf | 9 ++++---- 3 files changed, 29 insertions(+), 6 deletions(-) create mode 100644 modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff diff --git a/modules.json b/modules.json index d9f0b83d..5bd172bc 100644 --- a/modules.json +++ b/modules.json @@ -35,7 +35,8 @@ }, "custom/getchromsizes": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "patch": "modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff" }, "deeptools/computematrix": { "branch": "master", diff --git a/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff b/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff new file mode 100644 index 00000000..18fda554 --- /dev/null +++ b/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff @@ -0,0 +1,23 @@ +Changes in module 'nf-core/custom/getchromsizes' +--- modules/nf-core/custom/getchromsizes/main.nf ++++ modules/nf-core/custom/getchromsizes/main.nf +@@ -8,13 +8,12 @@ + 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + + input: +- tuple val(meta), path(fasta) ++ path(fasta) + + output: +- tuple val(meta), path ("*.sizes"), emit: sizes +- tuple val(meta), path ("*.fai") , emit: fai +- tuple val(meta), path ("*.gzi") , emit: gzi, optional: true +- path "versions.yml" , emit: versions ++ path '*.sizes' , emit: sizes ++ path '*.fai' , emit: fai ++ path "versions.yml", emit: versions + + when: + task.ext.when == null || task.ext.when + +************************************************************ diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf index 8e1693d4..562c693e 100644 --- a/modules/nf-core/custom/getchromsizes/main.nf +++ b/modules/nf-core/custom/getchromsizes/main.nf @@ -8,13 +8,12 @@ process CUSTOM_GETCHROMSIZES { 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" input: - tuple val(meta), path(fasta) + path(fasta) output: - tuple val(meta), path ("*.sizes"), emit: sizes - tuple val(meta), path ("*.fai") , emit: fai - tuple val(meta), path ("*.gzi") , emit: gzi, optional: true - path "versions.yml" , emit: versions + path '*.sizes' , emit: sizes + path '*.fai' , emit: fai + path "versions.yml", emit: versions when: task.ext.when == null || task.ext.when From 50ca23a4beea355fd512a043c0092cc0d5e91124 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 Oct 2022 15:50:26 +0200 Subject: [PATCH 006/201] Update custom_dumpsoftwareversions module --- .../custom/dumpsoftwareversions/main.nf | 8 +- .../templates/dumpsoftwareversions.py | 102 ++++++++++-------- 2 files changed, 60 insertions(+), 50 deletions(-) mode change 100644 => 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 203e485a..cebb6e05 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -1,11 +1,11 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_low' + label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda (params.enable_conda ? 'bioconda::multiqc=1.13a' : null) + conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.13a--pyhdfd78af_1' : - 'quay.io/biocontainers/multiqc:1.13a--pyhdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py old mode 100644 new mode 100755 index 787bdb7b..da033408 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -1,12 +1,16 @@ #!/usr/bin/env python -import platform -from textwrap import dedent + +"""Provide functions to merge multiple versions.yml files.""" + import yaml +import platform +from textwrap import dedent def _make_versions_html(versions): + """Generate a tabular HTML output of all versions for MultiQC.""" html = [ dedent( """\\ @@ -45,47 +49,53 @@ def _make_versions_html(versions): return "\\n".join(html) -versions_this_module = {} -versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, -} - -with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - -# aggregate versions by the module name (derived from fully-qualified process name) -versions_by_module = {} -for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - -versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", -} - -versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), -} - -with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) -with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - -with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) +def main(): + """Load all version files and generate merged output.""" + versions_this_module = {} + versions_this_module["${task.process}"] = { + "python": platform.python_version(), + "yaml": yaml.__version__, + } + + with open("$versions") as f: + versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + + # aggregate versions by the module name (derived from fully-qualified process name) + versions_by_module = {} + for process, process_versions in versions_by_process.items(): + module = process.split(":")[-1] + try: + if versions_by_module[module] != process_versions: + raise AssertionError( + "We assume that software versions are the same between all modules. " + "If you see this error-message it means you discovered an edge-case " + "and should open an issue in nf-core/tools. " + ) + except KeyError: + versions_by_module[module] = process_versions + + versions_by_module["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version", + } + + versions_mqc = { + "id": "software_versions", + "section_name": "${workflow.manifest.name} Software Versions", + "section_href": "https://github.com/${workflow.manifest.name}", + "plot_type": "html", + "description": "are collected at run time from the software output.", + "data": _make_versions_html(versions_by_module), + } + + with open("software_versions.yml", "w") as f: + yaml.dump(versions_by_module, f, default_flow_style=False) + with open("software_versions_mqc.yml", "w") as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + + with open("versions.yml", "w") as f: + yaml.dump(versions_this_module, f, default_flow_style=False) + + +if __name__ == "__main__": + main() From 9d70ec53f3af057045bbc6ba7734511377d9600a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 5 Oct 2022 16:50:18 +0200 Subject: [PATCH 007/201] Fix modules path after restructuring of nf-core/modules --- subworkflows/local/prepare_genome.nf | 14 +-- subworkflows/nf-core/align_bowtie2.nf | 2 +- subworkflows/nf-core/align_bwa_mem.nf | 2 +- subworkflows/nf-core/align_chromap.nf | 2 +- subworkflows/nf-core/bam_sort_samtools.nf | 4 +- subworkflows/nf-core/bam_stats_samtools.nf | 6 +- subworkflows/nf-core/fastqc_trimgalore.nf | 4 +- .../nf-core/mark_duplicates_picard.nf | 4 +- workflows/chipseq.nf | 86 +++++++++---------- 9 files changed, 62 insertions(+), 62 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 9b6422c6..6c18b41b 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -7,19 +7,19 @@ include { GUNZIP as GUNZIP_GTF GUNZIP as GUNZIP_GFF GUNZIP as GUNZIP_GENE_BED - GUNZIP as GUNZIP_BLACKLIST } from '../../modules/nf-core/modules/gunzip/main' + GUNZIP as GUNZIP_BLACKLIST } from '../../modules/nf-core/gunzip/main' include { UNTAR as UNTAR_BWA_INDEX UNTAR as UNTAR_BOWTIE2_INDEX UNTAR as UNTAR_CHROMAP_INDEX - UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/modules/untar/main' + UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/untar/main' -include { GFFREAD } from '../../modules/nf-core/modules/gffread/main' -include { CUSTOM_GETCHROMSIZES } from '../../modules/nf-core/modules/custom/getchromsizes/main' -include { BWA_INDEX } from '../../modules/nf-core/modules/bwa/index/main' -include { BOWTIE2_BUILD } from '../../modules/nf-core/modules/bowtie2/build/main' -include { CHROMAP_INDEX } from '../../modules/nf-core/modules/chromap/index/main' +include { GFFREAD } from '../../modules/nf-core/gffread/main' +include { CUSTOM_GETCHROMSIZES } from '../../modules/nf-core/custom/getchromsizes/main' +include { BWA_INDEX } from '../../modules/nf-core/bwa/index/main' +include { BOWTIE2_BUILD } from '../../modules/nf-core/bowtie2/build/main' +include { CHROMAP_INDEX } from '../../modules/nf-core/chromap/index/main' include { GTF2BED } from '../../modules/local/gtf2bed' include { GENOME_BLACKLIST_REGIONS } from '../../modules/local/genome_blacklist_regions' diff --git a/subworkflows/nf-core/align_bowtie2.nf b/subworkflows/nf-core/align_bowtie2.nf index 35219131..4661c720 100644 --- a/subworkflows/nf-core/align_bowtie2.nf +++ b/subworkflows/nf-core/align_bowtie2.nf @@ -2,7 +2,7 @@ * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats */ -include { BOWTIE2_ALIGN } from '../../modules/nf-core/modules/bowtie2/align/main' +include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' workflow ALIGN_BOWTIE2 { diff --git a/subworkflows/nf-core/align_bwa_mem.nf b/subworkflows/nf-core/align_bwa_mem.nf index 0c5dff08..eac98188 100644 --- a/subworkflows/nf-core/align_bwa_mem.nf +++ b/subworkflows/nf-core/align_bwa_mem.nf @@ -2,7 +2,7 @@ * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats */ -include { BWA_MEM } from '../../modules/nf-core/modules/bwa/mem/main' +include { BWA_MEM } from '../../modules/nf-core/bwa/mem/main' include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' workflow ALIGN_BWA_MEM { diff --git a/subworkflows/nf-core/align_chromap.nf b/subworkflows/nf-core/align_chromap.nf index 7eb73977..29b77442 100644 --- a/subworkflows/nf-core/align_chromap.nf +++ b/subworkflows/nf-core/align_chromap.nf @@ -2,7 +2,7 @@ * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats */ -include { CHROMAP_CHROMAP } from '../../modules/nf-core/modules/chromap/chromap/main' +include { CHROMAP_CHROMAP } from '../../modules/nf-core/chromap/chromap/main' include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' workflow ALIGN_CHROMAP { diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf index 418e14cc..deb06c4d 100644 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ b/subworkflows/nf-core/bam_sort_samtools.nf @@ -2,8 +2,8 @@ * Sort, index BAM file and run samtools stats, flagstat and idxstats */ -include { SAMTOOLS_SORT } from '../../modules/nf-core/modules/samtools/sort/main' -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' +include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' workflow BAM_SORT_SAMTOOLS { diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf index 89a7338f..207c5ece 100644 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ b/subworkflows/nf-core/bam_stats_samtools.nf @@ -2,9 +2,9 @@ * Run SAMtools stats, flagstat and idxstats */ -include { SAMTOOLS_STATS } from '../../modules/nf-core/modules/samtools/stats/main' -include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/modules/samtools/idxstats/main' -include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/modules/samtools/flagstat/main' +include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' +include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/samtools/idxstats/main' +include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main' workflow BAM_STATS_SAMTOOLS { take: diff --git a/subworkflows/nf-core/fastqc_trimgalore.nf b/subworkflows/nf-core/fastqc_trimgalore.nf index 966541ca..8a18098a 100644 --- a/subworkflows/nf-core/fastqc_trimgalore.nf +++ b/subworkflows/nf-core/fastqc_trimgalore.nf @@ -2,8 +2,8 @@ // Read QC and trimming // -include { FASTQC } from '../../modules/nf-core/modules/fastqc/main' -include { TRIMGALORE } from '../../modules/nf-core/modules/trimgalore/main' +include { FASTQC } from '../../modules/nf-core/fastqc/main' +include { TRIMGALORE } from '../../modules/nf-core/trimgalore/main' workflow FASTQC_TRIMGALORE { take: diff --git a/subworkflows/nf-core/mark_duplicates_picard.nf b/subworkflows/nf-core/mark_duplicates_picard.nf index 33e88bf5..72a0eb8e 100644 --- a/subworkflows/nf-core/mark_duplicates_picard.nf +++ b/subworkflows/nf-core/mark_duplicates_picard.nf @@ -2,8 +2,8 @@ * Picard MarkDuplicates, sort, index BAM file and run samtools stats, flagstat and idxstats */ -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/modules/picard/markduplicates/main' -include { SAMTOOLS_INDEX } from '../../modules/nf-core/modules/samtools/index/main' +include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/picard/markduplicates/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' workflow MARK_DUPLICATES_PICARD { diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index ffe99518..7be0f832 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -92,22 +92,22 @@ include { FILTER_BAM_BAMTOOLS } from '../subworkflows/local/filter_bam_bamtools' // MODULE: Installed directly from nf-core/modules // -include { PICARD_MERGESAMFILES } from '../modules/nf-core/modules/picard/mergesamfiles/main' -include { PICARD_COLLECTMULTIPLEMETRICS } from '../modules/nf-core/modules/picard/collectmultiplemetrics/main' -include { PRESEQ_LCEXTRAP } from '../modules/nf-core/modules/preseq/lcextrap/main' -include { PHANTOMPEAKQUALTOOLS } from '../modules/nf-core/modules/phantompeakqualtools/main' -include { UCSC_BEDGRAPHTOBIGWIG } from '../modules/nf-core/modules/ucsc/bedgraphtobigwig/main' -include { DEEPTOOLS_COMPUTEMATRIX } from '../modules/nf-core/modules/deeptools/computematrix/main' -include { DEEPTOOLS_PLOTPROFILE } from '../modules/nf-core/modules/deeptools/plotprofile/main' -include { DEEPTOOLS_PLOTHEATMAP } from '../modules/nf-core/modules/deeptools/plotheatmap/main' -include { DEEPTOOLS_PLOTFINGERPRINT } from '../modules/nf-core/modules/deeptools/plotfingerprint/main' -include { KHMER_UNIQUEKMERS } from '../modules/nf-core/modules/khmer/uniquekmers/main' -include { MACS2_CALLPEAK } from '../modules/nf-core/modules/macs2/callpeak/main' -include { SUBREAD_FEATURECOUNTS } from '../modules/nf-core/modules/subread/featurecounts/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/modules/custom/dumpsoftwareversions/main' - -include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_MACS2 } from '../modules/nf-core/modules/homer/annotatepeaks/main' -include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../modules/nf-core/modules/homer/annotatepeaks/main' +include { PICARD_MERGESAMFILES } from '../modules/nf-core/picard/mergesamfiles/main' +include { PICARD_COLLECTMULTIPLEMETRICS } from '../modules/nf-core/picard/collectmultiplemetrics/main' +include { PRESEQ_LCEXTRAP } from '../modules/nf-core/preseq/lcextrap/main' +include { PHANTOMPEAKQUALTOOLS } from '../modules/nf-core/phantompeakqualtools/main' +include { UCSC_BEDGRAPHTOBIGWIG } from '../modules/nf-core/ucsc/bedgraphtobigwig/main' +include { DEEPTOOLS_COMPUTEMATRIX } from '../modules/nf-core/deeptools/computematrix/main' +include { DEEPTOOLS_PLOTPROFILE } from '../modules/nf-core/deeptools/plotprofile/main' +include { DEEPTOOLS_PLOTHEATMAP } from '../modules/nf-core/deeptools/plotheatmap/main' +include { DEEPTOOLS_PLOTFINGERPRINT } from '../modules/nf-core/deeptools/plotfingerprint/main' +include { KHMER_UNIQUEKMERS } from '../modules/nf-core/khmer/uniquekmers/main' +include { MACS2_CALLPEAK } from '../modules/nf-core/macs2/callpeak/main' +include { SUBREAD_FEATURECOUNTS } from '../modules/nf-core/subread/featurecounts/main' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' + +include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_MACS2 } from '../modules/nf-core/homer/annotatepeaks/main' +include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../modules/nf-core/homer/annotatepeaks/main' // // SUBWORKFLOW: Consisting entirely of nf-core/modules @@ -226,7 +226,7 @@ workflow CHIPSEQ { ch_genome_bam_chromap .paired_end .collect() - .map { + .map { it -> def count = it.size() if (count > 0) { @@ -274,12 +274,12 @@ workflow CHIPSEQ { def meta_clone = meta.clone() meta_clone.remove('read_group') meta_clone.id = meta_clone.id.split('_')[0..-2].join('_') - [ meta_clone, bam ] + [ meta_clone, bam ] } .groupTuple(by: [0]) - .map { + .map { it -> - [ it[0], it[1].flatten() ] + [ it[0], it[1].flatten() ] } .set { ch_sort_bam } @@ -405,15 +405,15 @@ workflow CHIPSEQ { .bam .join(FILTER_BAM_BAMTOOLS.out.bai, by: [0]) .set { ch_genome_bam_bai } - + ch_genome_bam_bai .combine(ch_genome_bam_bai) - .map { + .map { meta1, bam1, bai1, meta2, bam2, bai2 -> meta1.control == meta2.id ? [ meta1, [ bam1, bam2 ], [ bai1, bai2 ] ] : null } .set { ch_ip_control_bam_bai } - + // // MODULE: deepTools plotFingerprint joint QC for IP and control // @@ -445,9 +445,9 @@ workflow CHIPSEQ { // Create channels: [ meta, ip_bam, control_bam ] ch_ip_control_bam_bai - .map { - meta, bams, bais -> - [ meta , bams[0], bams[1] ] + .map { + meta, bams, bais -> + [ meta , bams[0], bams[1] ] } .set { ch_ip_control_bam } @@ -472,9 +472,9 @@ workflow CHIPSEQ { // Create channels: [ meta, ip_bam, peaks ] ch_ip_control_bam .join(ch_macs2_peaks, by: [0]) - .map { - it -> - [ it[0], it[1], it[3] ] + .map { + it -> + [ it[0], it[1], it[3] ] } .set { ch_ip_bam_peaks } @@ -489,9 +489,9 @@ workflow CHIPSEQ { // Create channels: [ meta, peaks, frip ] ch_ip_bam_peaks .join(FRIP_SCORE.out.txt, by: [0]) - .map { - it -> - [ it[0], it[2], it[3] ] + .map { + it -> + [ it[0], it[2], it[3] ] } .set { ch_ip_peaks_frip } @@ -550,9 +550,9 @@ workflow CHIPSEQ { // Create channels: [ meta , [ peaks ] ] // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] ch_macs2_peaks - .map { - meta, peak -> - [ meta.antibody, meta.id.split('_')[0..-2].join('_'), peak ] + .map { + meta, peak -> + [ meta.antibody, meta.id.split('_')[0..-2].join('_'), peak ] } .groupTuple() .map { @@ -561,7 +561,7 @@ workflow CHIPSEQ { antibody, groups.groupBy().collectEntries { [(it.key) : it.value.size()] }, peaks - ] + ] } .map { antibody, groups, peaks -> @@ -569,7 +569,7 @@ workflow CHIPSEQ { meta_new.id = antibody meta_new.multiple_groups = groups.size() > 1 meta_new.replicates_exist = groups.max { groups.value }.value > 1 - [ meta_new, peaks ] + [ meta_new, peaks ] } .set { ch_antibody_peaks } @@ -605,7 +605,7 @@ workflow CHIPSEQ { // Create channels: [ antibody, [ ip_bams ] ] ch_ip_control_bam - .map { + .map { meta, ip_bam, control_bam -> [ meta.antibody, ip_bam ] } @@ -616,9 +616,9 @@ workflow CHIPSEQ { MACS2_CONSENSUS .out .saf - .map { - meta, saf -> - [ meta.id, meta, saf ] + .map { + meta, saf -> + [ meta.id, meta, saf ] } .join(ch_antibody_bams) .map { @@ -705,10 +705,10 @@ workflow CHIPSEQ { ch_picardcollectmultiplemetrics_multiqc.collect{it[1]}.ifEmpty([]), ch_preseq_multiqc.collect{it[1]}.ifEmpty([]), - + ch_deeptoolsplotprofile_multiqc.collect{it[1]}.ifEmpty([]), ch_deeptoolsplotfingerprint_multiqc.collect{it[1]}.ifEmpty([]), - + PHANTOMPEAKQUALTOOLS.out.spp.collect{it[1]}.ifEmpty([]), MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.nsc.collect{it[1]}.ifEmpty([]), MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.rsc.collect{it[1]}.ifEmpty([]), From c417d0967d484b333fe5ae0f85ec7af6e1d8e7b3 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 6 Oct 2022 11:54:48 +0200 Subject: [PATCH 008/201] Force modules update so that nf-core lint does not complain --- modules/nf-core/bwa/index/main.nf | 2 +- modules/nf-core/gunzip/main.nf | 2 +- modules/nf-core/preseq/lcextrap/main.nf | 2 +- modules/nf-core/preseq/lcextrap/meta.yml | 0 modules/nf-core/samtools/flagstat/main.nf | 4 +-- modules/nf-core/samtools/idxstats/main.nf | 1 + modules/nf-core/samtools/sort/main.nf | 1 + modules/nf-core/samtools/sort/meta.yml | 4 +++ modules/nf-core/samtools/stats/main.nf | 4 +-- modules/nf-core/trimgalore/main.nf | 2 +- modules/nf-core/ucsc/bedgraphtobigwig/main.nf | 2 +- .../nf-core/ucsc/bedgraphtobigwig/meta.yml | 0 modules/nf-core/untar/main.nf | 25 +++++++++++++------ modules/nf-core/untar/meta.yml | 8 +++--- 14 files changed, 38 insertions(+), 19 deletions(-) mode change 100644 => 100755 modules/nf-core/preseq/lcextrap/meta.yml mode change 100644 => 100755 modules/nf-core/ucsc/bedgraphtobigwig/meta.yml diff --git a/modules/nf-core/bwa/index/main.nf b/modules/nf-core/bwa/index/main.nf index 3affbf16..aa75ae5d 100644 --- a/modules/nf-core/bwa/index/main.nf +++ b/modules/nf-core/bwa/index/main.nf @@ -1,6 +1,6 @@ process BWA_INDEX { tag "$fasta" - label 'process_high' + label 'process_single' conda (params.enable_conda ? "bioconda::bwa=0.7.17" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf index 70367049..fa6ba26a 100644 --- a/modules/nf-core/gunzip/main.nf +++ b/modules/nf-core/gunzip/main.nf @@ -1,6 +1,6 @@ process GUNZIP { tag "$archive" - label 'process_low' + label 'process_single' conda (params.enable_conda ? "conda-forge::sed=4.7" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? diff --git a/modules/nf-core/preseq/lcextrap/main.nf b/modules/nf-core/preseq/lcextrap/main.nf index 97261557..a98a922c 100644 --- a/modules/nf-core/preseq/lcextrap/main.nf +++ b/modules/nf-core/preseq/lcextrap/main.nf @@ -1,6 +1,6 @@ process PRESEQ_LCEXTRAP { tag "$meta.id" - label 'process_medium' + label 'process_single' label 'error_ignore' conda (params.enable_conda ? "bioconda::preseq=3.1.2" : null) diff --git a/modules/nf-core/preseq/lcextrap/meta.yml b/modules/nf-core/preseq/lcextrap/meta.yml old mode 100644 new mode 100755 diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf index 03ec2dcf..c3152aca 100644 --- a/modules/nf-core/samtools/flagstat/main.nf +++ b/modules/nf-core/samtools/flagstat/main.nf @@ -1,6 +1,6 @@ process SAMTOOLS_FLAGSTAT { tag "$meta.id" - label 'process_low' + label 'process_single' conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? @@ -23,7 +23,7 @@ process SAMTOOLS_FLAGSTAT { """ samtools \\ flagstat \\ - --threads ${task.cpus-1} \\ + --threads ${task.cpus} \\ $bam \\ > ${prefix}.flagstat diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf index 4b245419..87618e5f 100644 --- a/modules/nf-core/samtools/idxstats/main.nf +++ b/modules/nf-core/samtools/idxstats/main.nf @@ -24,6 +24,7 @@ process SAMTOOLS_IDXSTATS { """ samtools \\ idxstats \\ + --threads ${task.cpus-1} \\ $bam \\ > ${prefix}.idxstats diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf index b4fc1cbe..ab7f1cca 100644 --- a/modules/nf-core/samtools/sort/main.nf +++ b/modules/nf-core/samtools/sort/main.nf @@ -12,6 +12,7 @@ process SAMTOOLS_SORT { output: tuple val(meta), path("*.bam"), emit: bam + tuple val(meta), path("*.csi"), emit: csi, optional: true path "versions.yml" , emit: versions when: diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml index a820c55a..09289751 100644 --- a/modules/nf-core/samtools/sort/meta.yml +++ b/modules/nf-core/samtools/sort/meta.yml @@ -39,6 +39,10 @@ output: type: file description: File containing software versions pattern: "versions.yml" + - csi: + type: file + description: BAM index file (optional) + pattern: "*.csi" authors: - "@drpatelh" - "@ewels" diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf index 89b92d79..9b0c3867 100644 --- a/modules/nf-core/samtools/stats/main.nf +++ b/modules/nf-core/samtools/stats/main.nf @@ -1,6 +1,6 @@ process SAMTOOLS_STATS { tag "$meta.id" - label 'process_low' + label 'process_single' conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? @@ -25,7 +25,7 @@ process SAMTOOLS_STATS { """ samtools \\ stats \\ - --threads ${task.cpus-1} \\ + --threads ${task.cpus} \\ ${reference} \\ ${input} \\ > ${prefix}.stats diff --git a/modules/nf-core/trimgalore/main.nf b/modules/nf-core/trimgalore/main.nf index 3a3fca90..a69e3de6 100644 --- a/modules/nf-core/trimgalore/main.nf +++ b/modules/nf-core/trimgalore/main.nf @@ -32,7 +32,7 @@ process TRIMGALORE { cores = (task.cpus as int) - 4 if (meta.single_end) cores = (task.cpus as int) - 3 if (cores < 1) cores = 1 - if (cores > 4) cores = 4 + if (cores > 8) cores = 8 } // Clipping presets have to be evaluated in the context of SE/PE diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf index b18b190a..a2979e04 100644 --- a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf +++ b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf @@ -1,6 +1,6 @@ process UCSC_BEDGRAPHTOBIGWIG { tag "$meta.id" - label 'process_medium' + label 'process_single' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. conda (params.enable_conda ? "bioconda::ucsc-bedgraphtobigwig=377" : null) diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml old mode 100644 new mode 100755 diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf index 29ab10a5..71eea7b2 100644 --- a/modules/nf-core/untar/main.nf +++ b/modules/nf-core/untar/main.nf @@ -1,6 +1,6 @@ process UNTAR { tag "$archive" - label 'process_low' + label 'process_single' conda (params.enable_conda ? "conda-forge::sed=4.7" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? @@ -25,12 +25,23 @@ process UNTAR { """ mkdir output - tar \\ - -C output --strip-components 1 \\ - -xzvf \\ - $args \\ - $archive \\ - $args2 + ## Ensures --strip-components only applied when top level of tar contents is a directory + ## If just files or multiple directories, place all in output + if [[ \$(tar -tzf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then + tar \\ + -C output --strip-components 1 \\ + -xzvf \\ + $args \\ + $archive \\ + $args2 + else + tar \\ + -C output \\ + -xzvf \\ + $args \\ + $archive \\ + $args2 + fi mv output ${untar} diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml index d426919b..ea7a3f38 100644 --- a/modules/nf-core/untar/meta.yml +++ b/modules/nf-core/untar/meta.yml @@ -26,9 +26,9 @@ output: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - untar: - type: file - description: - pattern: "*.*" + type: directory + description: Directory containing contents of archive + pattern: "*/" - versions: type: file description: File containing software versions @@ -36,3 +36,5 @@ output: authors: - "@joseespinosa" - "@drpatelh" + - "@matthdsm" + - "@jfy133" From 0fc1b938a0ce3b414fa8aed2b1b2347a6d8e0044 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 6 Oct 2022 14:14:11 +0200 Subject: [PATCH 009/201] Merged changes from nf-core template --- .github/workflows/awsfulltest.yml | 4 ++ .github/workflows/awstest.yml | 4 ++ .prettierignore | 1 + CITATION.cff | 8 +-- assets/adaptivecard.json | 67 +++++++++++++++++++++++++ assets/methods_description_template.yml | 25 +++++++++ assets/multiqc_config.yml | 6 ++- docs/usage.md | 8 +++ lib/NfcoreTemplate.groovy | 55 ++++++++++++++++++++ lib/Utils.groovy | 21 +++++--- lib/WorkflowChipseq.groovy | 19 +++++++ modules/local/multiqc.nf | 2 + modules/nf-core/multiqc/main.nf | 53 +++++++++++++++++++ nextflow.config | 6 ++- nextflow_schema.json | 18 +++++++ workflows/chipseq.nf | 14 +++++- 16 files changed, 295 insertions(+), 16 deletions(-) create mode 100644 assets/adaptivecard.json create mode 100644 assets/methods_description_template.yml mode change 100755 => 100644 lib/Utils.groovy create mode 100644 modules/nf-core/multiqc/main.nf diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 5a1c3622..85133f57 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -29,3 +29,7 @@ jobs: "aligner": "${{ matrix.aligner }}" } profiles: test_full,aws_tower + - uses: actions/upload-artifact@v3 + with: + name: Tower debug log file + path: tower_action_*.log diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 73640cc6..ecd16998 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -23,3 +23,7 @@ jobs: "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/chipseq/results-test-${{ github.sha }}" } profiles: test,aws_tower + - uses: actions/upload-artifact@v3 + with: + name: Tower debug log file + path: tower_action_*.log diff --git a/.prettierignore b/.prettierignore index d0e7ae58..eb74a574 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,4 +1,5 @@ email_template.html +adaptivecard.json .nextflow* work/ data/ diff --git a/CITATION.cff b/CITATION.cff index 4533e2f2..017666c0 100644 --- a/CITATION.cff +++ b/CITATION.cff @@ -13,8 +13,8 @@ authors: given-names: Johannes - family-names: Wilm given-names: Andreas - - family-names: Ulysse Garcia - given-names: Maxime + - family-names: Garcia + given-names: Maxime Ulysse - family-names: Di Tommaso given-names: Paolo - family-names: Nahnsen @@ -39,8 +39,8 @@ prefered-citation: given-names: Johannes - family-names: Wilm given-names: Andreas - - family-names: Ulysse Garcia - given-names: Maxime + - family-names: Garcia + given-names: Maxime Ulysse - family-names: Di Tommaso given-names: Paolo - family-names: Nahnsen diff --git a/assets/adaptivecard.json b/assets/adaptivecard.json new file mode 100644 index 00000000..8c6fb5a7 --- /dev/null +++ b/assets/adaptivecard.json @@ -0,0 +1,67 @@ +{ + "type": "message", + "attachments": [ + { + "contentType": "application/vnd.microsoft.card.adaptive", + "contentUrl": null, + "content": { + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "msteams": { + "width": "Full" + }, + "type": "AdaptiveCard", + "version": "1.2", + "body": [ + { + "type": "TextBlock", + "size": "Large", + "weight": "Bolder", + "color": "<% if (success) { %>Good<% } else { %>Attention<%} %>", + "text": "nf-core/chipseq v${version} - ${runName}", + "wrap": true + }, + { + "type": "TextBlock", + "spacing": "None", + "text": "Completed at ${dateComplete} (duration: ${duration})", + "isSubtle": true, + "wrap": true + }, + { + "type": "TextBlock", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors. The full error message was: ${errorReport}.<% } %>", + "wrap": true + }, + { + "type": "TextBlock", + "text": "The command used to launch the workflow was as follows:", + "wrap": true + }, + { + "type": "TextBlock", + "text": "${commandLine}", + "isSubtle": true, + "wrap": true + } + ], + "actions": [ + { + "type": "Action.ShowCard", + "title": "Pipeline Configuration", + "card": { + "type": "AdaptiveCard", + "\$schema": "http://adaptivecards.io/schemas/adaptive-card.json", + "body": [ + { + "type": "FactSet", + "facts": [<% out << summary.collect{ k,v -> "{\"title\": \"$k\", \"value\" : \"$v\"}"}.join(",\n") %> + ] + } + ] + } + } + ] + } + } + ] +} diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml new file mode 100644 index 00000000..a30b1eb6 --- /dev/null +++ b/assets/methods_description_template.yml @@ -0,0 +1,25 @@ +id: "nf-core-chipseq-methods-description" +description: "Suggested text and references to use when describing pipeline usage within the methods section of a publication." +section_name: "nf-core/chipseq Methods Description" +section_href: "https://github.com/nf-core/chipseq" +plot_type: "html" +## TODO nf-core: Update the HTML below to your prefered methods description, e.g. add publication citation for this pipeline +## You inject any metadata in the Nextflow '${workflow}' object +data: | +

    Methods

    +

    Data was processed using nf-core/chipseq v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020).

    +

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    + 
    ${workflow.commandLine}
    +

    References

    +
      +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. https://doi.org/10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. https://doi.org/10.1038/s41587-020-0439-x
      • +
    +
    +
    Notes:
    +
      + ${nodoi_text} +
    • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
      • +
    • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.
      • +
    +
    diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 4493905e..ada4bd5f 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -129,10 +129,12 @@ report_section_order: order: -3400 deseq2_clustering_10: order: -3500 - software_versions: + "nf-core-chipseq-methods-description": order: -3600 - nf-core-chipseq-summary: + software_versions: order: -3700 + "nf-core-chipseq-summary": + order: -3800 custom_plot_config: picard_insert_size: diff --git a/docs/usage.md b/docs/usage.md index 4a656032..bb6dcc52 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -296,6 +296,14 @@ See the main [Nextflow documentation](https://www.nextflow.io/docs/latest/config If you have any questions or issues please send us a message on [Slack](https://nf-co.re/join/slack) on the [`#configs` channel](https://nfcore.slack.com/channels/configs). +## Azure Resource Requests + +To be used with the `azurebatch` profile by specifying the `-profile azurebatch`. +We recommend providing a compute `params.vm_type` of `Standard_D16_v3` VMs by default but these options can be changed if required. + +Note that the choice of VM size depends on your quota and the overall workload during the analysis. +For a thorough list, please refer the [Azure Sizes for virtual machines in Azure](https://docs.microsoft.com/en-us/azure/virtual-machines/sizes). + ## Running in the background Nextflow handles job submissions and supervises the running jobs. The Nextflow process must run until the pipeline is finished. diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 2fc0a9b9..27feb009 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -145,6 +145,61 @@ class NfcoreTemplate { output_tf.withWriter { w -> w << email_txt } } + // + // Construct and send adaptive card + // https://adaptivecards.io + // + public static void adaptivecard(workflow, params, summary_params, projectDir, log) { + def hook_url = params.hook_url + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = workflow.manifest.version + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + def hf = new File("$projectDir/assets/adaptivecard.json") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } + } + // // Print pipeline summary on completion // diff --git a/lib/Utils.groovy b/lib/Utils.groovy old mode 100755 new mode 100644 index 28567bd7..8d030f4e --- a/lib/Utils.groovy +++ b/lib/Utils.groovy @@ -21,19 +21,26 @@ class Utils { } // Check that all channels are present - def required_channels = ['conda-forge', 'bioconda', 'defaults'] - def conda_check_failed = !required_channels.every { ch -> ch in channels } + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean // Check that they are in the right order - conda_check_failed |= !(channels.indexOf('conda-forge') < channels.indexOf('bioconda')) - conda_check_failed |= !(channels.indexOf('bioconda') < channels.indexOf('defaults')) + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } - if (conda_check_failed) { + if (channels_missing | channel_priority_violation) { log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + " There is a problem with your Conda configuration!\n\n" + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/user/install.html#set-up-channels\n" + - " NB: The order of the channels matters!\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" } } diff --git a/lib/WorkflowChipseq.groovy b/lib/WorkflowChipseq.groovy index dfae806f..ec6e85fb 100755 --- a/lib/WorkflowChipseq.groovy +++ b/lib/WorkflowChipseq.groovy @@ -2,6 +2,8 @@ // This file holds several functions specific to the workflow/chipseq.nf in the nf-core/chipseq pipeline // +import groovy.text.SimpleTemplateEngine + class WorkflowChipseq { // @@ -67,6 +69,23 @@ class WorkflowChipseq { yaml_file_text += "data: |\n" yaml_file_text += "${summary_section}" return yaml_file_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = run_workflow.toMap() + meta["manifest_map"] = run_workflow.manifest.toMap() + + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + def methods_text = mqc_methods_yaml.text + + def engine = new SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html }// // Exit pipeline if incorrect --genome key provided // diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index 702b239f..27dd8f29 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -11,6 +11,8 @@ process MULTIQC { path mqc_custom_config path software_versions path workflow_summary + path methods_description + path logo path ('fastqc/*') path ('trimgalore/fastqc/*') diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf new file mode 100644 index 00000000..a8159a57 --- /dev/null +++ b/modules/nf-core/multiqc/main.nf @@ -0,0 +1,53 @@ +process MULTIQC { + label 'process_single' + + conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + + input: + path multiqc_files, stageAs: "?/*" + path(multiqc_config) + path(extra_multiqc_config) + path(multiqc_logo) + + output: + path "*multiqc_report.html", emit: report + path "*_data" , emit: data + path "*_plots" , optional:true, emit: plots + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def config = multiqc_config ? "--config $multiqc_config" : '' + def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + """ + multiqc \\ + --force \\ + $args \\ + $config \\ + $extra_config \\ + . + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ + + stub: + """ + touch multiqc_data + touch multiqc_plots + touch multiqc_report.html + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + multiqc: \$( multiqc --version | sed -e "s/multiqc, version //g" ) + END_VERSIONS + """ +} diff --git a/nextflow.config b/nextflow.config index da798582..f50ebd04 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,9 @@ params { // MultiQC options multiqc_config = null multiqc_title = null + multiqc_logo = null max_multiqc_email_size = '25.MB' + multiqc_methods_description = null // Boilerplate options outdir = null @@ -81,6 +83,7 @@ params { email_on_fail = null plaintext_email = false monochrome_logs = false + hook_url = null help = false validate_params = true show_hidden_params = false @@ -232,7 +235,8 @@ manifest { description = 'ChIP-seq peak-calling and differential analysis pipeline.' mainScript = 'main.nf' nextflowVersion = '!>=21.10.3' - version = '2.1.0dev' + version = '2.1.0dev' + doi = 'https://doi.org/10.5281/zenodo.3240506' } // Load modules.config for DSL2 module specific options diff --git a/nextflow_schema.json b/nextflow_schema.json index 2a5f445c..e07ba6a6 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -528,12 +528,30 @@ "fa_icon": "fas fa-palette", "hidden": true }, + "hook_url": { + "type": "string", + "description": "Incoming hook URL for messaging service", + "fa_icon": "fas fa-people-group", + "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", + "hidden": true + }, "multiqc_config": { "type": "string", "description": "Custom config file to supply to MultiQC.", "fa_icon": "fas fa-cog", "hidden": true }, + "multiqc_logo": { + "type": "string", + "description": "Custom logo file to supply to MultiQC. File name must also be set in the MultiQC config file", + "fa_icon": "fas fa-image", + "hidden": true + }, + "multiqc_methods_description": { + "type": "string", + "description": "Custom MultiQC yaml file containing HTML including a methods description.", + "fa_icon": "fas fa-cog" + }, "tracedir": { "type": "string", "description": "Directory to keep pipeline Nextflow logs and reports.", diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 7be0f832..eaca622b 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -40,8 +40,10 @@ if (anno_readme && file(anno_readme).exists()) { ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -ch_multiqc_config = file("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config) : Channel.empty() +ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) +ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() +ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() +ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) // JSON files required by BAMTools for alignment filtering ch_bamtools_filter_se_config = file(params.bamtools_filter_se_config, checkIfExists: true) @@ -680,11 +682,16 @@ workflow CHIPSEQ { workflow_summary = WorkflowChipseq.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) + methods_description = WorkflowChipseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description) + ch_methods_description = Channel.value(methods_description) + MULTIQC ( ch_multiqc_config, ch_multiqc_custom_config.collect().ifEmpty([]), CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(), ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'), + ch_methods_description.collectFile(name: 'methods_description_mqc.yaml'), + ch_multiqc_logo.collect().ifEmpty([]), FASTQC_TRIMGALORE.out.fastqc_zip.collect{it[1]}.ifEmpty([]), FASTQC_TRIMGALORE.out.trim_zip.collect{it[1]}.ifEmpty([]), @@ -737,6 +744,9 @@ workflow.onComplete { NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) } NfcoreTemplate.summary(workflow, params, log) + if (params.hook_url) { + NfcoreTemplate.adaptivecard(workflow, params, summary_params, projectDir, log) + } } /* From ddf3e6b8093401502ef561e26c1d63caa55c0ca7 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 6 Oct 2022 14:41:54 +0200 Subject: [PATCH 010/201] Reorder onComplete snippet --- workflows/chipseq.nf | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index eaca622b..54fcd44b 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -743,10 +743,12 @@ workflow.onComplete { if (params.email || params.email_on_fail) { NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) } - NfcoreTemplate.summary(workflow, params, log) + if (params.hook_url) { NfcoreTemplate.adaptivecard(workflow, params, summary_params, projectDir, log) } + + NfcoreTemplate.summary(workflow, params, log) } /* From 3f548fe5b5eb2997a4a0ab5419b4c9f63e6bd23b Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 8 Dec 2022 13:09:28 +0000 Subject: [PATCH 011/201] Template update for nf-core/tools version 2.7.1 --- .devcontainer/devcontainer.json | 27 +++++++++++++ .gitattributes | 1 + .github/CONTRIBUTING.md | 16 ++++++++ .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- .github/workflows/ci.yml | 8 +++- .github/workflows/fix-linting.yml | 6 +-- .github/workflows/linting.yml | 18 +++++---- .github/workflows/linting_comment.yml | 2 +- .prettierignore | 2 + CHANGELOG.md | 2 +- CITATION.cff | 56 --------------------------- README.md | 4 +- assets/slackreport.json | 34 ++++++++++++++++ docs/usage.md | 24 +++++++----- lib/NfcoreSchema.groovy | 1 - lib/NfcoreTemplate.groovy | 41 +++++++++++++++----- lib/WorkflowMain.groovy | 18 ++++++--- modules.json | 9 +++-- modules/local/samplesheet_check.nf | 4 ++ nextflow.config | 14 +++++-- nextflow_schema.json | 8 +++- workflows/chipseq.nf | 11 +++--- 22 files changed, 195 insertions(+), 113 deletions(-) create mode 100644 .devcontainer/devcontainer.json delete mode 100644 CITATION.cff create mode 100644 assets/slackreport.json diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json new file mode 100644 index 00000000..ea27a584 --- /dev/null +++ b/.devcontainer/devcontainer.json @@ -0,0 +1,27 @@ +{ + "name": "nfcore", + "image": "nfcore/gitpod:latest", + "remoteUser": "gitpod", + + // Configure tool-specific properties. + "customizations": { + // Configure properties specific to VS Code. + "vscode": { + // Set *default* container specific settings.json values on container create. + "settings": { + "python.defaultInterpreterPath": "/opt/conda/bin/python", + "python.linting.enabled": true, + "python.linting.pylintEnabled": true, + "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", + "python.formatting.yapfPath": "/opt/conda/bin/yapf", + "python.linting.flake8Path": "/opt/conda/bin/flake8", + "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", + "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", + "python.linting.pylintPath": "/opt/conda/bin/pylint" + }, + + // Add the IDs of extensions you want installed when the container is created. + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } +} diff --git a/.gitattributes b/.gitattributes index 050bb120..7a2dabc2 100644 --- a/.gitattributes +++ b/.gitattributes @@ -1,3 +1,4 @@ *.config linguist-language=nextflow +*.nf.test linguist-language=nextflow modules/nf-core/** linguist-generated subworkflows/nf-core/** linguist-generated diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 2bfaa681..5d23c1d9 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -101,3 +101,19 @@ If you are using a new feature from core Nextflow, you may bump the minimum requ ### Images and figures For overview images and other documents we follow the nf-core [style guidelines and examples](https://nf-co.re/developers/design_guidelines). + +## GitHub Codespaces + +This repo includes a devcontainer configuration which will create a GitHub Codespaces for Nextflow development! This is an online developer environment that runs in your browser, complete with VSCode and a terminal. + +To get started: + +- Open the repo in [Codespaces](https://github.com/nf-core/chipseq/codespaces) +- Tools installed + - nf-core + - Nextflow + +Devcontainer specs: + +- [DevContainer config](.devcontainer/devcontainer.json) +- [Dockerfile](.devcontainer/Dockerfile) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 1d09e717..99d3b9bf 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -42,7 +42,7 @@ body: attributes: label: System information description: | - * Nextflow version _(eg. 21.10.3)_ + * Nextflow version _(eg. 22.10.1)_ * Hardware _(eg. HPC, Desktop, Cloud)_ * Executor _(eg. slurm, local, awsbatch)_ * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_ diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index cf99db7f..fb742746 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -11,6 +11,10 @@ on: env: NXF_ANSI_LOG: false +concurrency: + group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" + cancel-in-progress: true + jobs: test: name: Run pipeline with test data @@ -20,11 +24,11 @@ jobs: strategy: matrix: NXF_VER: - - "21.10.3" + - "22.10.1" - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v2 + uses: actions/checkout@v3 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index b4f90734..f99a8155 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -24,7 +24,7 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v2 + - uses: actions/setup-node@v3 - name: Install Prettier run: npm install -g prettier @prettier/plugin-php @@ -34,9 +34,9 @@ jobs: id: prettier_status run: | if prettier --check ${GITHUB_WORKSPACE}; then - echo "::set-output name=result::pass" + echo "name=result::pass" >> $GITHUB_OUTPUT else - echo "::set-output name=result::fail" + echo "name=result::fail" >> $GITHUB_OUTPUT fi - name: Run 'prettier --write' diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 8a5ce69b..858d622e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -4,6 +4,8 @@ name: nf-core linting # that the code meets the nf-core guidelines. on: push: + branches: + - dev pull_request: release: types: [published] @@ -12,9 +14,9 @@ jobs: EditorConfig: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - - uses: actions/setup-node@v2 + - uses: actions/setup-node@v3 - name: Install editorconfig-checker run: npm install -g editorconfig-checker @@ -25,9 +27,9 @@ jobs: Prettier: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - - uses: actions/setup-node@v2 + - uses: actions/setup-node@v3 - name: Install Prettier run: npm install -g prettier @@ -38,7 +40,7 @@ jobs: PythonBlack: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v2 + - uses: actions/checkout@v3 - name: Check code lints with Black uses: psf/black@stable @@ -69,12 +71,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v2 + uses: actions/checkout@v3 - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v3 + - uses: actions/setup-python@v4 with: python-version: "3.7" architecture: "x64" @@ -97,7 +99,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v2 + uses: actions/upload-artifact@v3 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 04758f61..39635186 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -18,7 +18,7 @@ jobs: - name: Get PR number id: pr_number - run: echo "::set-output name=pr_number::$(cat linting-logs/PR_number.txt)" + run: echo "name=pr_number::$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment uses: marocchino/sticky-pull-request-comment@v2 diff --git a/.prettierignore b/.prettierignore index eb74a574..437d763d 100644 --- a/.prettierignore +++ b/.prettierignore @@ -1,5 +1,6 @@ email_template.html adaptivecard.json +slackreport.json .nextflow* work/ data/ @@ -8,3 +9,4 @@ results/ testing/ testing* *.pyc +bin/ diff --git a/CHANGELOG.md b/CHANGELOG.md index ddd11538..89107f2b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.0.0 - [date] +## v2.1.0dev - [date] Initial release of nf-core/chipseq, created with the [nf-core](https://nf-co.re/) template. diff --git a/CITATION.cff b/CITATION.cff deleted file mode 100644 index 017666c0..00000000 --- a/CITATION.cff +++ /dev/null @@ -1,56 +0,0 @@ -cff-version: 1.2.0 -message: "If you use `nf-core tools` in your work, please cite the `nf-core` publication" -authors: - - family-names: Ewels - given-names: Philip - - family-names: Peltzer - given-names: Alexander - - family-names: Fillinger - given-names: Sven - - family-names: Patel - given-names: Harshil - - family-names: Alneberg - given-names: Johannes - - family-names: Wilm - given-names: Andreas - - family-names: Garcia - given-names: Maxime Ulysse - - family-names: Di Tommaso - given-names: Paolo - - family-names: Nahnsen - given-names: Sven -title: "The nf-core framework for community-curated bioinformatics pipelines." -version: 2.4.1 -doi: 10.1038/s41587-020-0439-x -date-released: 2022-05-16 -url: https://github.com/nf-core/tools -prefered-citation: - type: article - authors: - - family-names: Ewels - given-names: Philip - - family-names: Peltzer - given-names: Alexander - - family-names: Fillinger - given-names: Sven - - family-names: Patel - given-names: Harshil - - family-names: Alneberg - given-names: Johannes - - family-names: Wilm - given-names: Andreas - - family-names: Garcia - given-names: Maxime Ulysse - - family-names: Di Tommaso - given-names: Paolo - - family-names: Nahnsen - given-names: Sven - doi: 10.1038/s41587-020-0439-x - journal: nature biotechnology - start: 276 - end: 278 - title: "The nf-core framework for community-curated bioinformatics pipelines." - issue: 3 - volume: 38 - year: 2020 - url: https://dx.doi.org/10.1038/s41587-020-0439-x diff --git a/README.md b/README.md index 0b1f436d..eae41b5f 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -31,7 +31,7 @@ On release, automated continuous integration tests run the pipeline on a full-si ## Quick Start -1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`) +1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_. diff --git a/assets/slackreport.json b/assets/slackreport.json new file mode 100644 index 00000000..043d02f2 --- /dev/null +++ b/assets/slackreport.json @@ -0,0 +1,34 @@ +{ + "attachments": [ + { + "fallback": "Plain-text summary of the attachment.", + "color": "<% if (success) { %>good<% } else { %>danger<%} %>", + "author_name": "sanger-tol/readmapping v${version} - ${runName}", + "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", + "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", + "fields": [ + { + "title": "Command used to launch the workflow", + "value": "```${commandLine}```", + "short": false + } + <% + if (!success) { %> + , + { + "title": "Full error message", + "value": "```${errorReport}```", + "short": false + }, + { + "title": "Pipeline configuration", + "value": "<% out << summary.collect{ k,v -> k == "hook_url" ? "_${k}_: (_hidden_)" : ( ( v.class.toString().contains('Path') || ( v.class.toString().contains('String') && v.contains('/') ) ) ? "_${k}_: `${v}`" : (v.class.toString().contains('DateTime') ? ("_${k}_: " + v.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM))) : "_${k}_: ${v}") ) }.join(",\n") %>", + "short": false + } + <% } + %> + ], + "footer": "Completed at <% out << dateComplete.format(java.time.format.DateTimeFormatter.ofLocalizedDateTime(java.time.format.FormatStyle.MEDIUM)) %> (duration: ${duration})" + } + ] +} diff --git a/docs/usage.md b/docs/usage.md index 776325ae..6ed6fb45 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -83,9 +83,9 @@ nextflow pull nf-core/chipseq It is a good idea to specify a pipeline version when running the pipeline on your data. This ensures that a specific version of the pipeline code and software are used when you run your pipeline. If you keep using the same tag, you'll be running the same version of the pipeline, even if there have been changes to the code since. -First, go to the [nf-core/chipseq releases page](https://github.com/nf-core/chipseq/releases) and find the latest version number - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. +First, go to the [nf-core/chipseq releases page](https://github.com/nf-core/chipseq/releases) and find the latest pipeline version - numeric only (eg. `1.3.1`). Then specify this when running the pipeline with `-r` (one hyphen) - eg. `-r 1.3.1`. Of course, you can switch to another version by changing the number after the `-r` flag. -This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. +This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. ## Core Nextflow arguments @@ -95,7 +95,7 @@ This version number will be logged in reports when you run the pipeline, so that Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. When using Biocontainers, most of these software packaging methods pull Docker containers from quay.io e.g [FastQC](https://quay.io/repository/biocontainers/fastqc) except for Singularity which directly downloads Singularity images via https hosted by the [Galaxy project](https://depot.galaxyproject.org/singularity/) and Conda which downloads and installs software locally from [Bioconda](https://bioconda.github.io/). +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. @@ -104,8 +104,11 @@ The pipeline also dynamically loads configurations from [https://github.com/nf-c Note that multiple profiles can be loaded, for example: `-profile test,docker` - the order of arguments is important! They are loaded in sequence, so later profiles can overwrite earlier profiles. -If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended. +If `-profile` is not specified, the pipeline will run locally and expect all software to be installed and available on the `PATH`. This is _not_ recommended, since it can lead to different results on different machines dependent on the computer enviroment. +- `test` + - A profile with a complete configuration for automated testing + - Includes links to test data so needs no other parameters - `docker` - A generic configuration profile to be used with [Docker](https://docker.com/) - `singularity` @@ -118,9 +121,6 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) - `conda` - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. -- `test` - - A profile with a complete configuration for automated testing - - Includes links to test data so needs no other parameters ### `-resume` @@ -169,8 +169,14 @@ Work dir: Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` ``` +#### For beginners + +A first step to bypass this error, you could try to increase the amount of CPUs, memory, and time for the whole pipeline. Therefor you can try to increase the resource for the parameters `--max_cpus`, `--max_memory`, and `--max_time`. Based on the error above, you have to increase the amount of memory. Therefore you can go to the [parameter documentation of rnaseq](https://nf-co.re/rnaseq/3.9/parameters) and scroll down to the `show hidden parameter` button to get the default value for `--max_memory`. In this case 128GB, you than can try to run your pipeline again with `--max_memory 200GB -resume` to skip all process, that were already calculated. If you can not increase the resource of the complete pipeline, you can try to adapt the resource for a single process as mentioned below. + +#### Advanced option on process level + To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). -We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/software/star/align/main.nf`. +We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/star/align/main.nf`. If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. @@ -189,7 +195,7 @@ process { > > If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly. -### Updating containers +### Updating containers (advanced users) The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`. diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index b3d092f8..33cd4f6e 100755 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -46,7 +46,6 @@ class NfcoreSchema { 'quiet', 'syslog', 'v', - 'version', // Options for `nextflow run` command 'ansi', diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 27feb009..25a0a74a 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -32,6 +32,25 @@ class NfcoreTemplate { } } + // + // Generate version string + // + public static String version(workflow) { + String version_string = "" + + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string + } + // // Construct and send completion email // @@ -61,7 +80,7 @@ class NfcoreTemplate { misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp def email_fields = [:] - email_fields['version'] = workflow.manifest.version + email_fields['version'] = NfcoreTemplate.version(workflow) email_fields['runName'] = workflow.runName email_fields['success'] = workflow.success email_fields['dateComplete'] = workflow.complete @@ -146,10 +165,10 @@ class NfcoreTemplate { } // - // Construct and send adaptive card - // https://adaptivecards.io + // Construct and send a notification to a web server as JSON + // e.g. Microsoft Teams and Slack // - public static void adaptivecard(workflow, params, summary_params, projectDir, log) { + public static void IM_notification(workflow, params, summary_params, projectDir, log) { def hook_url = params.hook_url def summary = [:] @@ -170,7 +189,7 @@ class NfcoreTemplate { misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp def msg_fields = [:] - msg_fields['version'] = workflow.manifest.version + msg_fields['version'] = NfcoreTemplate.version(workflow) msg_fields['runName'] = workflow.runName msg_fields['success'] = workflow.success msg_fields['dateComplete'] = workflow.complete @@ -178,13 +197,16 @@ class NfcoreTemplate { msg_fields['exitStatus'] = workflow.exitStatus msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") msg_fields['projectDir'] = workflow.projectDir msg_fields['summary'] = summary << misc_fields // Render the JSON template def engine = new groovy.text.GStringTemplateEngine() - def hf = new File("$projectDir/assets/adaptivecard.json") + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("$projectDir/assets/${json_path}") def json_template = engine.createTemplate(hf).make(msg_fields) def json_message = json_template.toString() @@ -209,7 +231,7 @@ class NfcoreTemplate { if (workflow.stats.ignoredCount == 0) { log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" } } else { log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" @@ -297,6 +319,7 @@ class NfcoreTemplate { // public static String logo(workflow, monochrome_logs) { Map colors = logColours(monochrome_logs) + String workflow_version = NfcoreTemplate.version(workflow) String.format( """\n ${dashedLine(monochrome_logs)} @@ -305,7 +328,7 @@ class NfcoreTemplate { ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} v${workflow.manifest.version}${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} ${dashedLine(monochrome_logs)} """.stripIndent() ) diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 904824ae..91ffb560 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -19,7 +19,7 @@ class WorkflowMain { } // - // Print help to screen if required + // Generate help string // public static String help(workflow, params, log) { def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" @@ -32,7 +32,7 @@ class WorkflowMain { } // - // Print parameter summary log to screen + // Generate parameter summary log string // public static String paramsSummaryLog(workflow, params, log) { def summary_log = '' @@ -53,15 +53,21 @@ class WorkflowMain { System.exit(0) } - // Validate workflow parameters via the JSON schema - if (params.validate_params) { - NfcoreSchema.validateParameters(workflow, params, log) + // Print workflow version and exit on --version + if (params.version) { + String workflow_version = NfcoreTemplate.version(workflow) + log.info "${workflow.manifest.name} ${workflow_version}" + System.exit(0) } // Print parameter summary log to screen - log.info paramsSummaryLog(workflow, params, log) + // Validate workflow parameters via the JSON schema + if (params.validate_params) { + NfcoreSchema.validateParameters(workflow, params, log) + } + // Check that a -profile or Nextflow config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) diff --git a/modules.json b/modules.json index cfa6d32f..5db2402e 100644 --- a/modules.json +++ b/modules.json @@ -7,15 +7,18 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905" + "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "installed_by": ["modules"] } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 5094e5a0..2b5892bd 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -1,5 +1,6 @@ process SAMPLESHEET_CHECK { tag "$samplesheet" + label 'process_single' conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? @@ -13,6 +14,9 @@ process SAMPLESHEET_CHECK { path '*.csv' , emit: csv path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ """ check_samplesheet.py \\ diff --git a/nextflow.config b/nextflow.config index fa8bfa21..dd16a37e 100644 --- a/nextflow.config +++ b/nextflow.config @@ -35,6 +35,7 @@ params { monochrome_logs = false hook_url = null help = false + version = false validate_params = true show_hidden_params = false schema_ignore_params = 'genomes' @@ -81,6 +82,7 @@ profiles { debug { process.beforeScript = 'echo $HOSTNAME' } conda { params.enable_conda = true + conda.enabled = true docker.enabled = false singularity.enabled = false podman.enabled = false @@ -89,6 +91,7 @@ profiles { } mamba { params.enable_conda = true + conda.enabled = true conda.useMamba = true docker.enabled = false singularity.enabled = false @@ -104,6 +107,9 @@ profiles { shifter.enabled = false charliecloud.enabled = false } + arm { + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + } singularity { singularity.enabled = true singularity.autoMounts = true @@ -185,12 +191,12 @@ dag { manifest { name = 'nf-core/chipseq' - author = 'Espinosa-Carrasco J, Patel H, Wang C, Ewels P' + author = """Espinosa-Carrasco J, Patel H, Wang C, Ewels P""" homePage = 'https://github.com/nf-core/chipseq' - description = 'ChIP-seq peak-calling and differential analysis pipeline.' + description = """ChIP-seq peak-calling and differential analysis pipeline.""" mainScript = 'main.nf' - nextflowVersion = '!>=21.10.3' - version = '2.0.0' + nextflowVersion = '!>=22.10.1' + version = '2.1.0dev' doi = '' } diff --git a/nextflow_schema.json b/nextflow_schema.json index dd7c26cf..cd6daca0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -176,6 +176,12 @@ "fa_icon": "fas fa-question-circle", "hidden": true }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, "publish_dir_mode": { "type": "string", "default": "copy", @@ -217,7 +223,7 @@ "type": "string", "description": "Incoming hook URL for messaging service", "fa_icon": "fas fa-people-group", - "help_text": "Incoming hook URL for messaging service. Currently, only MS Teams is supported.", + "help_text": "Incoming hook URL for messaging service. Currently, MS Teams and Slack are supported.", "hidden": true }, "multiqc_config": { diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index a39a34d2..c0645263 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -82,7 +82,7 @@ workflow CHIPSEQ { ch_versions = ch_versions.mix(FASTQC.out.versions.first()) CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') + ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') ) // @@ -102,12 +102,11 @@ workflow CHIPSEQ { MULTIQC ( ch_multiqc_files.collect(), - ch_multiqc_config.collect().ifEmpty([]), - ch_multiqc_custom_config.collect().ifEmpty([]), - ch_multiqc_logo.collect().ifEmpty([]) + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList() ) multiqc_report = MULTIQC.out.report.toList() - ch_versions = ch_versions.mix(MULTIQC.out.versions) } /* @@ -122,7 +121,7 @@ workflow.onComplete { } NfcoreTemplate.summary(workflow, params, log) if (params.hook_url) { - NfcoreTemplate.adaptivecard(workflow, params, summary_params, projectDir, log) + NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) } } From 8913497f785bfcc8a73171e7439c0717eff5c5cc Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 19 Dec 2022 12:04:59 +0000 Subject: [PATCH 012/201] Template update for nf-core/tools version 2.7.2 --- .github/workflows/fix-linting.yml | 4 +- .github/workflows/linting_comment.yml | 2 +- lib/WorkflowMain.groovy | 2 +- modules.json | 6 +- modules/local/samplesheet_check.nf | 2 +- .../custom/dumpsoftwareversions/main.nf | 2 +- .../templates/dumpsoftwareversions.py | 99 ++++++++++--------- modules/nf-core/fastqc/main.nf | 40 +++----- modules/nf-core/multiqc/main.nf | 2 +- nextflow.config | 3 - nextflow_schema.json | 6 -- workflows/chipseq.nf | 2 +- 12 files changed, 82 insertions(+), 88 deletions(-) mode change 100644 => 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index f99a8155..c695df98 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -34,9 +34,9 @@ jobs: id: prettier_status run: | if prettier --check ${GITHUB_WORKSPACE}; then - echo "name=result::pass" >> $GITHUB_OUTPUT + echo "result=pass" >> $GITHUB_OUTPUT else - echo "name=result::fail" >> $GITHUB_OUTPUT + echo "result=fail" >> $GITHUB_OUTPUT fi - name: Run 'prettier --write' diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 39635186..0bbcd30f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -18,7 +18,7 @@ jobs: - name: Get PR number id: pr_number - run: echo "name=pr_number::$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT + run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment uses: marocchino/sticky-pull-request-comment@v2 diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 91ffb560..bfe9c5ea 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -72,7 +72,7 @@ class WorkflowMain { NfcoreTemplate.checkConfigProvided(workflow, log) // Check that conda channels are set-up correctly - if (params.enable_conda) { + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { Utils.checkCondaChannels(log) } diff --git a/modules.json b/modules.json index 5db2402e..6e4968b6 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 2b5892bd..5d5d6afb 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -2,7 +2,7 @@ process SAMPLESHEET_CHECK { tag "$samplesheet" label 'process_single' - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3' : 'quay.io/biocontainers/python:3.8.3' }" diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index cebb6e05..3df21765 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,7 +2,7 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null) + conda "bioconda::multiqc=1.13" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py old mode 100644 new mode 100755 index 787bdb7b..e55b8d43 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py @@ -1,5 +1,9 @@ #!/usr/bin/env python + +"""Provide functions to merge multiple versions.yml files.""" + + import platform from textwrap import dedent @@ -7,6 +11,7 @@ def _make_versions_html(versions): + """Generate a tabular HTML output of all versions for MultiQC.""" html = [ dedent( """\\ @@ -45,47 +50,53 @@ def _make_versions_html(versions): return "\\n".join(html) -versions_this_module = {} -versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, -} - -with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - -# aggregate versions by the module name (derived from fully-qualified process name) -versions_by_module = {} -for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - -versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", -} - -versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), -} - -with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) -with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - -with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) +def main(): + """Load all version files and generate merged output.""" + versions_this_module = {} + versions_this_module["${task.process}"] = { + "python": platform.python_version(), + "yaml": yaml.__version__, + } + + with open("$versions") as f: + versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module + + # aggregate versions by the module name (derived from fully-qualified process name) + versions_by_module = {} + for process, process_versions in versions_by_process.items(): + module = process.split(":")[-1] + try: + if versions_by_module[module] != process_versions: + raise AssertionError( + "We assume that software versions are the same between all modules. " + "If you see this error-message it means you discovered an edge-case " + "and should open an issue in nf-core/tools. " + ) + except KeyError: + versions_by_module[module] = process_versions + + versions_by_module["Workflow"] = { + "Nextflow": "$workflow.nextflow.version", + "$workflow.manifest.name": "$workflow.manifest.version", + } + + versions_mqc = { + "id": "software_versions", + "section_name": "${workflow.manifest.name} Software Versions", + "section_href": "https://github.com/${workflow.manifest.name}", + "plot_type": "html", + "description": "are collected at run time from the software output.", + "data": _make_versions_html(versions_by_module), + } + + with open("software_versions.yml", "w") as f: + yaml.dump(versions_by_module, f, default_flow_style=False) + with open("software_versions_mqc.yml", "w") as f: + yaml.dump(versions_mqc, f, default_flow_style=False) + + with open("versions.yml", "w") as f: + yaml.dump(versions_this_module, f, default_flow_style=False) + + +if __name__ == "__main__": + main() diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 05730368..9ae58381 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,7 +2,7 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::fastqc=0.11.9" : null) + conda "bioconda::fastqc=0.11.9" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : 'quay.io/biocontainers/fastqc:0.11.9--0' }" @@ -20,30 +20,22 @@ process FASTQC { script: def args = task.ext.args ?: '' - // Add soft-links to original FastQs for consistent naming in pipeline def prefix = task.ext.prefix ?: "${meta.id}" - if (meta.single_end) { - """ - [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz - fastqc $args --threads $task.cpus ${prefix}.fastq.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) - END_VERSIONS - """ - } else { - """ - [ ! -f ${prefix}_1.fastq.gz ] && ln -s ${reads[0]} ${prefix}_1.fastq.gz - [ ! -f ${prefix}_2.fastq.gz ] && ln -s ${reads[1]} ${prefix}_2.fastq.gz - fastqc $args --threads $task.cpus ${prefix}_1.fastq.gz ${prefix}_2.fastq.gz - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) - END_VERSIONS - """ - } + // Make list of old name and new name pairs to use for renaming in the bash while loop + def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } + def rename_to = old_new_pairs*.join(' ').join(' ') + def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + """ + printf "%s %s\\n" $rename_to | while read old_name new_name; do + [ -f "\${new_name}" ] || ln -s \$old_name \$new_name + done + fastqc $args --threads $task.cpus $renamed_files + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + END_VERSIONS + """ stub: def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index a8159a57..68f66bea 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,7 +1,7 @@ process MULTIQC { label 'process_single' - conda (params.enable_conda ? 'bioconda::multiqc=1.13' : null) + conda "bioconda::multiqc=1.13" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" diff --git a/nextflow.config b/nextflow.config index dd16a37e..8f2539d1 100644 --- a/nextflow.config +++ b/nextflow.config @@ -39,7 +39,6 @@ params { validate_params = true show_hidden_params = false schema_ignore_params = 'genomes' - enable_conda = false // Config options @@ -81,7 +80,6 @@ try { profiles { debug { process.beforeScript = 'echo $HOSTNAME' } conda { - params.enable_conda = true conda.enabled = true docker.enabled = false singularity.enabled = false @@ -90,7 +88,6 @@ profiles { charliecloud.enabled = false } mamba { - params.enable_conda = true conda.enabled = true conda.useMamba = true docker.enabled = false diff --git a/nextflow_schema.json b/nextflow_schema.json index cd6daca0..29c14519 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -263,12 +263,6 @@ "description": "Show all params when using `--help`", "hidden": true, "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." - }, - "enable_conda": { - "type": "boolean", - "description": "Run this workflow with Conda. You can also use '-profile conda' instead of providing this parameter.", - "hidden": true, - "fa_icon": "fas fa-bacon" } } } diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index c0645263..f43f0a6f 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -82,7 +82,7 @@ workflow CHIPSEQ { ch_versions = ch_versions.mix(FASTQC.out.versions.first()) CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique{ it.text }.collectFile(name: 'collated_versions.yml') + ch_versions.unique().collectFile(name: 'collated_versions.yml') ) // From 0ea0d5bfcda50c6c1014b2c8a24765c91695c971 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Dec 2022 14:29:17 +0100 Subject: [PATCH 013/201] Update changelog --- CHANGELOG.md | 2 ++ 1 file changed, 2 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 03f988a3..8b41ec17 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -7,6 +7,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Enhancements & fixes +- Updated pipeline template to [nf-core/tools 2.7.2](https://github.com/nf-core/tools/releases/tag/2.7.2) + ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 ### Enhancements & fixes From 6e106dd4bde4682b2d4e1beb6a9687d3e9607964 Mon Sep 17 00:00:00 2001 From: Sarah Guinchard Date: Thu, 5 Jan 2023 16:34:43 +0100 Subject: [PATCH 014/201] Add metro map files + embed image in readme --- README.md | 2 + .../images/nf-core-chipseq_metro_map_grey.png | Bin 0 -> 203370 bytes .../images/nf-core-chipseq_metro_map_grey.svg | 2799 +++++++++++++++++ 3 files changed, 2801 insertions(+) create mode 100644 docs/images/nf-core-chipseq_metro_map_grey.png create mode 100644 docs/images/nf-core-chipseq_metro_map_grey.svg diff --git a/README.md b/README.md index 519a93eb..7c5467c7 100644 --- a/README.md +++ b/README.md @@ -26,6 +26,8 @@ You can find numerous talks on the [nf-core events page](https://nf-co.re/events ## Pipeline summary +![nf-core/chipseq metro map](docs/images/nf-core-chipseq_metro_map_grey.png) + 1. Raw read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/)) 3. 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Picard Mark duplicatesMerge alignments from multiple libraries of the same sample SAMtoolsBEDtoolsBAMtools (paired-ends only)Pysam (paired-ends only) + + License: + + + + + + + + + + + + + + + + + + + From 860a044201ba3dc14e0ee672801ae25b26cb9783 Mon Sep 17 00:00:00 2001 From: Sarah Guinchard Date: Thu, 5 Jan 2023 16:52:31 +0100 Subject: [PATCH 015/201] Added name in credits --- README.md | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index 41153384..c4ce42cd 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3240506-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3240506) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -64,7 +64,7 @@ You can find numerous talks on the [nf-core events page](https://nf-co.re/events ## Quick Start -1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`) +1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_. @@ -97,6 +97,8 @@ The nf-core/chipseq pipeline comes with documentation about the pipeline: [usage These scripts were originally written by Chuan Wang ([@chuan-wang](https://github.com/chuan-wang)) and Phil Ewels ([@ewels](https://github.com/ewels)) for use at the [National Genomics Infrastructure](https://portal.scilifelab.se/genomics/) at [SciLifeLab](http://www.scilifelab.se/) in Stockholm, Sweden. The pipeline was re-implemented by Harshil Patel ([@drpatelh](https://github.com/drpatelh)) from [Seqera Labs, Spain](https://seqera.io/) and converted to Nextflow DSL2 by Jose Espinosa-Carrasco ([@JoseEspinosa](https://github.com/JoseEspinosa)) from [The Comparative Bioinformatics Group](https://www.crg.eu/en/cedric_notredame) at [The Centre for Genomic Regulation, Spain](https://www.crg.eu/). +The pipeline workflow diagram was designe by Sarah Guinchard ([@G-Sarah](https://github.com/G-Sarah)). + Many thanks to others who have helped out and contributed along the way too, including (but not limited to): [@apeltzer](https://github.com/apeltzer), [@bc2zb](https://github.com/bc2zb), [@crickbabs](https://github.com/crickbabs), [@drejom](https://github.com/drejom), [@houghtos](https://github.com/houghtos), [@KevinMenden](https://github.com/KevinMenden), [@mashehu](https://github.com/mashehu), [@pditommaso](https://github.com/pditommaso), [@Rotholandus](https://github.com/Rotholandus), [@sofiahaglund](https://github.com/sofiahaglund), [@tiagochst](https://github.com/tiagochst) and [@winni2k](https://github.com/winni2k). ## Contributions and Support From 9bc62ef78076b40bfaf8f8aade083163906cd606 Mon Sep 17 00:00:00 2001 From: Sarah Guinchard Date: Thu, 5 Jan 2023 16:52:46 +0100 Subject: [PATCH 016/201] Updated changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8b41ec17..491f4296 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Enhancements & fixes - Updated pipeline template to [nf-core/tools 2.7.2](https://github.com/nf-core/tools/releases/tag/2.7.2) +- [[#317](https://github.com/nf-core/chipseq/issues/317)] Added metro map ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 From 183b641799adbf546d14763cd5f3f0d47faa2a0f Mon Sep 17 00:00:00 2001 From: Sarah Guinchard <99276764+G-Sarah@users.noreply.github.com> Date: Thu, 5 Jan 2023 17:14:33 +0100 Subject: [PATCH 017/201] corrected nextflow versions --- README.md | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/README.md b/README.md index c4ce42cd..232ef8b8 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.3240506-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.3240506) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A521.10.3-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -64,7 +64,7 @@ You can find numerous talks on the [nf-core events page](https://nf-co.re/events ## Quick Start -1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=21.10.3`) +1. Install [`Nextflow`](https://www.nextflow.io/docs/latest/getstarted.html#installation) (`>=22.10.1`) 2. Install any of [`Docker`](https://docs.docker.com/engine/installation/), [`Singularity`](https://www.sylabs.io/guides/3.0/user-guide/) (you can follow [this tutorial](https://singularity-tutorial.github.io/01-installation/)), [`Podman`](https://podman.io/), [`Shifter`](https://nersc.gitlab.io/development/shifter/how-to-use/) or [`Charliecloud`](https://hpc.github.io/charliecloud/) for full pipeline reproducibility _(you can use [`Conda`](https://conda.io/miniconda.html) both to install Nextflow itself and also to manage software within pipelines. Please only use it within pipelines as a last resort; see [docs](https://nf-co.re/usage/configuration#basic-configuration-profiles))_. 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style="font-variant:normal;font-weight:normal;font-size:11.00666809px;line-height:0%;font-family:'Liberation Sans';-inkscape-font-specification:LiberationSans;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:0.35277781" x="398.3688" - y="-202.90166"> - - + style="stroke-width:0.35277781" /> @@ -717,7 +715,8 @@ id="flowPara4861-7-7" style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:17.5px;line-height:1.25;font-family:'Eurostile Extended';-inkscape-font-specification:'Eurostile Extended';text-align:start;text-anchor:start"> Germline Germline + Sarek    +  Somatic Somatic + Sarek    + Sarek    +  Exome Exome +  Germline Germline + Sarek    +  Somatic Somatic + Sarek    + Sarek    +  Exome Exome + r r + fasta + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:32px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">fasta + @@ -1370,7 +1382,8 @@ width="112" id="rect10549-8" />GFF + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:32px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">GFF + @@ -1403,7 +1416,8 @@ width="112" id="rect10549-6" />fastq + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:32px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">fastq + @@ -1436,7 +1450,8 @@ width="112" id="rect10549-2-3" />HTML + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:32px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">HTML + Cutadapt + id="tspan1347" + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.3499999px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.26458299" /> + BAM + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:32px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">BAM + @@ -1689,7 +1706,8 @@ width="112" id="rect10549-3-4" />BAI + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:32px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">BAI + @@ -1723,7 +1741,8 @@ width="112" id="rect10549-9-1" />bigWig + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:26.23099518px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">bigWig + Picard¹ + id="tspan1347-9" + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.3499999px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.26458299" /> Picard + id="tspan1347-9-6" + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.3499999px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.26458299" /> Preseq + id="tspan1347-9-0" + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.3499999px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.26458299" /> BedgraphtoBigWig + id="tspan1347-9-0-2" + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.3499999px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.26458299" /> PreseqBedtools + y="-5.3573666" + id="tspan1347-9-0-24" + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.35px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.264583" /> @@ -2557,7 +2576,8 @@ width="85.200035" id="rect10549-9-1-6" />XML + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:26.23099518px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold';text-align:center;direction:rtl;text-anchor:middle">XML + tab + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:32px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold';text-align:center;text-anchor:middle">tab + 1. 1. + 2. 2. + Merge alignments from multiple libraries of the same sample + BAMtools (paired-ends only)Pysam (paired-ends only) Pysam (paired-ends only) + Date: Thu, 19 Jan 2023 10:34:28 +0000 Subject: [PATCH 019/201] correcting link to blacklist directory --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index be4ed6f8..77a094e3 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -138,7 +138,7 @@ "blacklist": { "type": "string", "description": "Path to blacklist regions in BED format, used for filtering alignments.", - "help_text": "If provided, alignments that overlap with the regions in this file will be filtered out (see [ENCODE blacklists](https://sites.google.com/site/anshulkundaje/projects/blacklists)). The file should be in BED format. Blacklisted regions for *GRCh37*, *GRCh38*, *GRCm38*, *hg19*, *hg38*, *mm10* are bundled with the pipeline in the [`blacklists`](../assets/blacklists/) directory, and as such will be automatically used if any of those genomes are specified with the `--genome` parameter.", + "help_text": "If provided, alignments that overlap with the regions in this file will be filtered out (see [ENCODE blacklists](https://sites.google.com/site/anshulkundaje/projects/blacklists)). The file should be in BED format. Blacklisted regions for *GRCh37*, *GRCh38*, *GRCm38*, *hg19*, *hg38*, *mm10* are bundled with the pipeline in the [`/assets/blacklists`](https://github.com/nf-core/chipseq/tree/master/assets/blacklists) directory, and as such will be automatically used if any of those genomes are specified with the `--genome` parameter.", "fa_icon": "fas fa-book-dead" }, "save_reference": { From 232ca305b77f6476b73fc3a36fbd8043d87745a4 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 10:14:43 +0200 Subject: [PATCH 020/201] Bump chromap version 0.2.4 --- modules.json | 4 ++-- modules/nf-core/chromap/chromap/main.nf | 10 +++++----- modules/nf-core/chromap/chromap/meta.yml | 8 +++++++- modules/nf-core/chromap/index/main.nf | 12 ++++++------ modules/nf-core/chromap/index/meta.yml | 13 ++++++++++++- 5 files changed, 32 insertions(+), 15 deletions(-) diff --git a/modules.json b/modules.json index a2d49adf..7af6cca7 100644 --- a/modules.json +++ b/modules.json @@ -27,12 +27,12 @@ }, "chromap/chromap": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", "installed_by": ["modules"] }, "chromap/index": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { diff --git a/modules/nf-core/chromap/chromap/main.nf b/modules/nf-core/chromap/chromap/main.nf index 137f0340..5b7631cb 100644 --- a/modules/nf-core/chromap/chromap/main.nf +++ b/modules/nf-core/chromap/chromap/main.nf @@ -2,15 +2,15 @@ process CHROMAP_CHROMAP { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::chromap=0.2.1 bioconda::samtools=1.15.1" : null) + conda "bioconda::chromap=0.2.4 bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:963e4fe6a85c548a4018585660aed79780a175d3-0' : - 'quay.io/biocontainers/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:963e4fe6a85c548a4018585660aed79780a175d3-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:5b2e433ab8b3d1ef098fc944b567fd98caa23f56-0' : + 'quay.io/biocontainers/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:5b2e433ab8b3d1ef098fc944b567fd98caa23f56-0' }" input: tuple val(meta), path(reads) - path fasta - path index + tuple val(meta2), path(fasta) + tuple val(meta2), path(index) path barcodes path whitelist path chr_order diff --git a/modules/nf-core/chromap/chromap/meta.yml b/modules/nf-core/chromap/chromap/meta.yml index a86fddc9..10ce8f58 100644 --- a/modules/nf-core/chromap/chromap/meta.yml +++ b/modules/nf-core/chromap/chromap/meta.yml @@ -20,7 +20,7 @@ tools: homepage: https://github.com/haowenz/chromap documentation: https://github.com/haowenz/chromap tool_dev_url: https://github.com/haowenz/chromap - doi: "" + licence: ["GPL v3"] input: - meta: @@ -33,6 +33,11 @@ input: description: | List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively. + - meta2: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test' ] - fasta: type: file description: | @@ -86,3 +91,4 @@ output: authors: - "@mahesh-panchal" + - "@joseespinosa" diff --git a/modules/nf-core/chromap/index/main.nf b/modules/nf-core/chromap/index/main.nf index ee370695..25ee6a7e 100644 --- a/modules/nf-core/chromap/index/main.nf +++ b/modules/nf-core/chromap/index/main.nf @@ -2,17 +2,17 @@ process CHROMAP_INDEX { tag "$fasta" label 'process_medium' - conda (params.enable_conda ? "bioconda::chromap=0.2.1" : null) + conda "bioconda::chromap=0.2.4" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/chromap:0.2.1--hd03093a_0' : - 'quay.io/biocontainers/chromap:0.2.1--hd03093a_0' }" + 'https://depot.galaxyproject.org/singularity/chromap:0.2.4--hd03093a_0' : + 'quay.io/biocontainers/chromap:0.2.4--hd03093a_0' }" input: - path fasta + tuple val(meta), path(fasta) output: - path "*.index" , emit: index - path "versions.yml", emit: versions + tuple val(meta), path ("*.index"), emit: index + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/chromap/index/meta.yml b/modules/nf-core/chromap/index/meta.yml index 6659221f..39c5459b 100644 --- a/modules/nf-core/chromap/index/meta.yml +++ b/modules/nf-core/chromap/index/meta.yml @@ -11,10 +11,15 @@ tools: homepage: https://github.com/haowenz/chromap documentation: https://github.com/haowenz/chromap tool_dev_url: https://github.com/haowenz/chromap - doi: "" + licence: ["GPL v3"] input: + - meta: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] - fasta: type: file description: Fasta reference file. @@ -24,6 +29,11 @@ output: type: file description: File containing software versions pattern: "versions.yml" + - meta: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] - index: type: file description: Index file of the reference genome @@ -31,3 +41,4 @@ output: authors: - "@mahesh-panchal" + - "@joseespinosa" From 91b3763f98748dd84cbad1327770ef10d066d980 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 10:18:41 +0200 Subject: [PATCH 021/201] Enable chromap for paired-end reads --- subworkflows/local/prepare_genome.nf | 4 ++-- workflows/chipseq.nf | 36 ++++------------------------ 2 files changed, 6 insertions(+), 34 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 6c18b41b..ed5377bf 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -176,10 +176,10 @@ workflow PREPARE_GENOME { ch_chromap_index = UNTAR_CHROMAP_INDEX ( [ [:], params.chromap_index ] ).untar.map{ it[1] } ch_versions = ch_versions.mix(UNTAR.out.versions) } else { - ch_chromap_index = file(params.chromap_index) + ch_chromap_index = [ [:], file(params.chromap_index) ] } } else { - ch_chromap_index = CHROMAP_INDEX ( ch_fasta ).index + ch_chromap_index = CHROMAP_INDEX ( [ [:], ch_fasta ] ).index ch_versions = ch_versions.mix(CHROMAP_INDEX.out.versions) } } diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 5d7c67af..08069eb6 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -208,39 +208,11 @@ workflow CHIPSEQ { FASTQC_TRIMGALORE.out.reads, PREPARE_GENOME.out.chromap_index, PREPARE_GENOME.out.fasta + .map { + [ [:], it ] + } ) - - // Filter out paired-end reads until the issue below is fixed - // https://github.com/nf-core/chipseq/issues/291 - // ch_genome_bam = ALIGN_CHROMAP.out.bam - ALIGN_CHROMAP - .out - .bam - .branch { - meta, bam -> - single_end: meta.single_end - return [ meta, bam ] - paired_end: !meta.single_end - return [ meta, bam ] - } - .set { ch_genome_bam_chromap } - - ch_genome_bam_chromap - .paired_end - .collect() - .map { - it -> - def count = it.size() - if (count > 0) { - log.warn "=============================================================================\n" + - " Paired-end files produced by chromap cannot be used by some downstream tools due to the issue below:\n" + - " https://github.com/nf-core/chipseq/issues/291\n" + - " They will be excluded from the analysis. Consider using a different aligner\n" + - "===================================================================================" - } - } - - ch_genome_bam = ch_genome_bam_chromap.single_end + ch_genome_bam = ALIGN_CHROMAP.out.bam ch_genome_bam_index = ALIGN_CHROMAP.out.bai ch_samtools_stats = ALIGN_CHROMAP.out.stats ch_samtools_flagstat = ALIGN_CHROMAP.out.flagstat From 595c679d59427bdff979b40d89da65230b48e221 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 10:39:38 +0200 Subject: [PATCH 022/201] Fix black --- bin/bampe_rm_orphan.py | 3 --- 1 file changed, 3 deletions(-) diff --git a/bin/bampe_rm_orphan.py b/bin/bampe_rm_orphan.py index 4ab9935b..ddba1d6f 100755 --- a/bin/bampe_rm_orphan.py +++ b/bin/bampe_rm_orphan.py @@ -46,7 +46,6 @@ def makedir(path): - if not len(path) == 0: try: os.makedirs(path) @@ -63,7 +62,6 @@ def makedir(path): def bampe_rm_orphan(BAMIn, BAMOut, onlyFRPairs=False): - ## SETUP DIRECTORY/FILE STRUCTURE OutDir = os.path.dirname(BAMOut) makedir(OutDir) @@ -89,7 +87,6 @@ def bampe_rm_orphan(BAMIn, BAMOut, onlyFRPairs=False): ## FILTER FOR READS ON SAME CHROMOSOME IN FR ORIENTATION if onlyFRPairs: if pair1.tid == pair2.tid: - ## READ1 FORWARD AND READ2 REVERSE STRAND if not pair1.is_reverse and pair2.is_reverse: if pair1.reference_start <= pair2.reference_start: From 8cda00ff923da42bd3141c10d0c0c215fba0a41a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 11:06:14 +0200 Subject: [PATCH 023/201] Make black happy again --- bin/check_samplesheet.py | 2 -- bin/igv_files_to_session.py | 2 -- bin/macs2_merged_expand.py | 2 -- 3 files changed, 6 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index eaf0d24c..3fd6ff8a 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -49,7 +49,6 @@ def check_samplesheet(file_in, file_out): sample_mapping_dict = {} with open(file_in, "r", encoding="utf-8-sig") as fin: - ## Check header MIN_COLS = 2 HEADER = ["sample", "fastq_1", "fastq_2", "antibody", "control"] @@ -156,7 +155,6 @@ def check_samplesheet(file_in, file_out): + "\n" ) for sample in sorted(sample_mapping_dict.keys()): - ## Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): print_error( diff --git a/bin/igv_files_to_session.py b/bin/igv_files_to_session.py index 629e6cc9..6fdcb1a9 100755 --- a/bin/igv_files_to_session.py +++ b/bin/igv_files_to_session.py @@ -55,7 +55,6 @@ def makedir(path): - if not len(path) == 0: try: os.makedirs(path) @@ -72,7 +71,6 @@ def makedir(path): def igv_files_to_session(XMLOut, ListFile, ReplaceFile, Genome, PathPrefix=""): - makedir(os.path.dirname(XMLOut)) replaceFileDict = {} diff --git a/bin/macs2_merged_expand.py b/bin/macs2_merged_expand.py index aa401123..28ffb087 100755 --- a/bin/macs2_merged_expand.py +++ b/bin/macs2_merged_expand.py @@ -55,7 +55,6 @@ def makedir(path): - if not len(path) == 0: try: os.makedirs(path) @@ -78,7 +77,6 @@ def makedir(path): def macs2_merged_expand(MergedIntervalTxtFile, SampleNameList, OutFile, isNarrow=False, minReplicates=1): - makedir(os.path.dirname(OutFile)) combFreqDict = {} From 261cc8640ee5a2dcac109a9bff652cfeb74f86f2 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 12:45:23 +0200 Subject: [PATCH 024/201] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 491f4296..8c56805d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,6 +9,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated pipeline template to [nf-core/tools 2.7.2](https://github.com/nf-core/tools/releases/tag/2.7.2) - [[#317](https://github.com/nf-core/chipseq/issues/317)] Added metro map +- [[#288](https://github.com/nf-core/chipseq/issues/291)] Bump `chromap` version 2 and enable all the steps below chromap again when paired-end data is processed. ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 From b4ad530dc270036271205f42110f116c22d03279 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 22:07:19 +0200 Subject: [PATCH 025/201] Add back skip_spp parameter --- CHANGELOG.md | 1 + workflows/chipseq.nf | 44 +++++++++++++++++++++++++++----------------- 2 files changed, 28 insertions(+), 17 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 8c56805d..173b68d1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated pipeline template to [nf-core/tools 2.7.2](https://github.com/nf-core/tools/releases/tag/2.7.2) - [[#317](https://github.com/nf-core/chipseq/issues/317)] Added metro map - [[#288](https://github.com/nf-core/chipseq/issues/291)] Bump `chromap` version 2 and enable all the steps below chromap again when paired-end data is processed. +- [[#311](https://github.com/nf-core/chipseq/issues/311)] Add back `--skip_spp` parameter which was unintentionally removed from the code. ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 08069eb6..caaa4c0c 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -310,20 +310,30 @@ workflow CHIPSEQ { // // MODULE: Phantompeaktools strand cross-correlation and QC metrics // - PHANTOMPEAKQUALTOOLS ( - FILTER_BAM_BAMTOOLS.out.bam - ) - ch_versions = ch_versions.mix(PHANTOMPEAKQUALTOOLS.out.versions.first()) + ch_phantompeakqualtools_spp_multiqc = Channel.empty() + ch_multiqc_phantompeakqualtools_nsc_multiqc = Channel.empty() + ch_multiqc_phantompeakqualtools_rsc_multiqc = Channel.empty() + ch_multiqc_phantompeakqualtools_correlation_multiqc = Channel.empty() + if (!params.skip_spp) { + PHANTOMPEAKQUALTOOLS ( + FILTER_BAM_BAMTOOLS.out.bam + ) + ch_phantompeakqualtools_spp_multiqc = PHANTOMPEAKQUALTOOLS.out.spp + ch_versions = ch_versions.mix(PHANTOMPEAKQUALTOOLS.out.versions.first()) - // - // MODULE: MultiQC custom content for Phantompeaktools - // - MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS ( - PHANTOMPEAKQUALTOOLS.out.spp.join(PHANTOMPEAKQUALTOOLS.out.rdata, by: [0]), - ch_spp_nsc_header, - ch_spp_rsc_header, - ch_spp_correlation_header - ) + // + // MODULE: MultiQC custom content for Phantompeaktools + // + MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS ( + PHANTOMPEAKQUALTOOLS.out.spp.join(PHANTOMPEAKQUALTOOLS.out.rdata, by: [0]), + ch_spp_nsc_header, + ch_spp_rsc_header, + ch_spp_correlation_header + ) + ch_multiqc_phantompeakqualtools_nsc_multiqc = MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.nsc + ch_multiqc_phantompeakqualtools_rsc_multiqc = MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.rsc + ch_multiqc_phantompeakqualtools_correlation_multiqc = MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.correlation + } // // MODULE: BedGraph coverage tracks @@ -688,10 +698,10 @@ workflow CHIPSEQ { ch_deeptoolsplotprofile_multiqc.collect{it[1]}.ifEmpty([]), ch_deeptoolsplotfingerprint_multiqc.collect{it[1]}.ifEmpty([]), - PHANTOMPEAKQUALTOOLS.out.spp.collect{it[1]}.ifEmpty([]), - MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.nsc.collect{it[1]}.ifEmpty([]), - MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.rsc.collect{it[1]}.ifEmpty([]), - MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS.out.correlation.collect{it[1]}.ifEmpty([]), + ch_phantompeakqualtools_spp_multiqc.collect{it[1]}.ifEmpty([]), + ch_multiqc_phantompeakqualtools_nsc_multiqc.collect{it[1]}.ifEmpty([]), + ch_multiqc_phantompeakqualtools_rsc_multiqc.collect{it[1]}.ifEmpty([]), + ch_multiqc_phantompeakqualtools_correlation_multiqc.collect{it[1]}.ifEmpty([]), ch_custompeaks_frip_multiqc.collect{it[1]}.ifEmpty([]), ch_custompeaks_count_multiqc.collect{it[1]}.ifEmpty([]), From 77907aa5ddf9f60cecc7d795bffb02d583708d6c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 22:44:59 +0200 Subject: [PATCH 026/201] Install fastq_fastqc_umitools_trimgalore subworkflow --- modules.json | 154 ++++++++++++++---- modules/nf-core/umitools/extract/main.nf | 56 +++++++ modules/nf-core/umitools/extract/meta.yml | 47 ++++++ .../fastq_fastqc_umitools_trimgalore/main.nf | 123 ++++++++++++++ .../fastq_fastqc_umitools_trimgalore/meta.yml | 96 +++++++++++ 5 files changed, 442 insertions(+), 34 deletions(-) create mode 100644 modules/nf-core/umitools/extract/main.nf create mode 100644 modules/nf-core/umitools/extract/meta.yml create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main.nf create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml diff --git a/modules.json b/modules.json index 7af6cca7..8f10866d 100644 --- a/modules.json +++ b/modules.json @@ -8,171 +8,257 @@ "bowtie2/align": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "chromap/chromap": { "branch": "master", "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "chromap/index": { "branch": "master", "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/getchromsizes": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"], + "installed_by": [ + "modules" + ], "patch": "modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff" }, "deeptools/computematrix": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "fastqc": { "branch": "master", "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "installed_by": [ + "modules", + "fastq_fastqc_umitools_trimgalore" + ] }, "gffread": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "macs2/callpeak": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "phantompeakqualtools": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/markduplicates": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "preseq/lcextrap": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/flagstat": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/idxstats": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/sort": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/stats": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "subread/featurecounts": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "trimgalore": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules", + "fastq_fastqc_umitools_trimgalore" + ] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] + }, + "umitools/extract": { + "branch": "master", + "git_sha": "ffe2f5865f608848e440a52b73c304ea79aaf818", + "installed_by": [ + "fastq_fastqc_umitools_trimgalore" + ] }, "untar": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] + } + } + }, + "subworkflows": { + "nf-core": { + "fastq_fastqc_umitools_trimgalore": { + "branch": "master", + "git_sha": "100caf3506850ffcc04953f724a97f422940c377", + "installed_by": [ + "subworkflows" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf new file mode 100644 index 00000000..64fdd367 --- /dev/null +++ b/modules/nf-core/umitools/extract/main.nf @@ -0,0 +1,56 @@ +process UMITOOLS_EXTRACT { + tag "$meta.id" + label "process_single" + label "process_long" + + conda "bioconda::umi_tools=1.1.4" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' : + 'quay.io/biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }" + + input: + tuple val(meta), path(reads) + + output: + tuple val(meta), path("*.fastq.gz"), emit: reads + tuple val(meta), path("*.log") , emit: log + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if (meta.single_end) { + """ + umi_tools \\ + extract \\ + -I $reads \\ + -S ${prefix}.umi_extract.fastq.gz \\ + $args \\ + > ${prefix}.umi_extract.log + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//') + END_VERSIONS + """ + } else { + """ + umi_tools \\ + extract \\ + -I ${reads[0]} \\ + --read2-in=${reads[1]} \\ + -S ${prefix}.umi_extract_1.fastq.gz \\ + --read2-out=${prefix}.umi_extract_2.fastq.gz \\ + $args \\ + > ${prefix}.umi_extract.log + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//') + END_VERSIONS + """ + } +} diff --git a/modules/nf-core/umitools/extract/meta.yml b/modules/nf-core/umitools/extract/meta.yml new file mode 100644 index 00000000..db64a0f8 --- /dev/null +++ b/modules/nf-core/umitools/extract/meta.yml @@ -0,0 +1,47 @@ +name: umitools_extract +description: Extracts UMI barcode from a read and add it to the read name, leaving any sample barcode in place +keywords: + - umitools + - extract +tools: + - umi_tools: + description: > + UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) + and single cell RNA-Seq cell barcodes + documentation: https://umi-tools.readthedocs.io/en/latest/ + license: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: list + description: | + List of input FASTQ files whose UMIs will be extracted. +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - reads: + type: file + description: > + Extracted FASTQ files. | + For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | + For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. + pattern: "*.{fastq.gz}" + - log: + type: file + description: Logfile for umi_tools + pattern: "*.{log}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + +authors: + - "@drpatelh" + - "@grst" diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main.nf b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main.nf new file mode 100644 index 00000000..db2e5b32 --- /dev/null +++ b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main.nf @@ -0,0 +1,123 @@ +// +// Read QC, UMI extraction and trimming +// + +include { FASTQC } from '../../../modules/nf-core/fastqc/main' +include { UMITOOLS_EXTRACT } from '../../../modules/nf-core/umitools/extract/main' +include { TRIMGALORE } from '../../../modules/nf-core/trimgalore/main' + +// +// Function that parses TrimGalore log output file to get total number of reads after trimming +// +def getTrimGaloreReadsAfterFiltering(log_file) { + def total_reads = 0 + def filtered_reads = 0 + log_file.eachLine { line -> + def total_reads_matcher = line =~ /([\d\.]+)\ssequences processed in total/ + def filtered_reads_matcher = line =~ /shorter than the length cutoff[^:]+:\s([\d\.]+)/ + if (total_reads_matcher) total_reads = total_reads_matcher[0][1].toFloat() + if (filtered_reads_matcher) filtered_reads = filtered_reads_matcher[0][1].toFloat() + } + return total_reads - filtered_reads +} + +workflow FASTQ_FASTQC_UMITOOLS_TRIMGALORE { + take: + reads // channel: [ val(meta), [ reads ] ] + skip_fastqc // boolean: true/false + with_umi // boolean: true/false + skip_umi_extract // boolean: true/false + skip_trimming // boolean: true/false + umi_discard_read // integer: 0, 1 or 2 + min_trimmed_reads // integer: > 0 + + main: + ch_versions = Channel.empty() + fastqc_html = Channel.empty() + fastqc_zip = Channel.empty() + if (!skip_fastqc) { + FASTQC (reads) + fastqc_html = FASTQC.out.html + fastqc_zip = FASTQC.out.zip + ch_versions = ch_versions.mix(FASTQC.out.versions.first()) + } + + umi_reads = reads + umi_log = Channel.empty() + if (with_umi && !skip_umi_extract) { + UMITOOLS_EXTRACT (reads) + umi_reads = UMITOOLS_EXTRACT.out.reads + umi_log = UMITOOLS_EXTRACT.out.log + ch_versions = ch_versions.mix(UMITOOLS_EXTRACT.out.versions.first()) + + // Discard R1 / R2 if required + if (umi_discard_read in [1,2]) { + UMITOOLS_EXTRACT + .out + .reads + .map { + meta, reads -> + meta.single_end ? [ meta, reads ] : [ meta + ['single_end': true], reads[umi_discard_read % 2] ] + } + .set { umi_reads } + } + } + + trim_reads = umi_reads + trim_unpaired = Channel.empty() + trim_html = Channel.empty() + trim_zip = Channel.empty() + trim_log = Channel.empty() + trim_read_count = Channel.empty() + if (!skip_trimming) { + TRIMGALORE (umi_reads) + trim_unpaired = TRIMGALORE.out.unpaired + trim_html = TRIMGALORE.out.html + trim_zip = TRIMGALORE.out.zip + trim_log = TRIMGALORE.out.log + ch_versions = ch_versions.mix(TRIMGALORE.out.versions.first()) + + // + // Filter FastQ files based on minimum trimmed read count after adapter trimming + // + TRIMGALORE + .out + .reads + .join(trim_log, remainder: true) + .map { + meta, reads, trim_log -> + if (trim_log) { + num_reads = getTrimGaloreReadsAfterFiltering(meta.single_end ? trim_log : trim_log[-1]) + [ meta, reads, num_reads ] + } else { + [ meta, reads, min_trimmed_reads.toFloat() + 1 ] + } + } + .set { ch_num_trimmed_reads } + + ch_num_trimmed_reads + .filter { meta, reads, num_reads -> num_reads >= min_trimmed_reads.toFloat() } + .map { meta, reads, num_reads -> [ meta, reads ] } + .set { trim_reads } + + ch_num_trimmed_reads + .map { meta, reads, num_reads -> [ meta, num_reads ] } + .set { trim_read_count } + } + + emit: + reads = trim_reads // channel: [ val(meta), [ reads ] ] + + fastqc_html // channel: [ val(meta), [ html ] ] + fastqc_zip // channel: [ val(meta), [ zip ] ] + + umi_log // channel: [ val(meta), [ log ] ] + + trim_unpaired // channel: [ val(meta), [ reads ] ] + trim_html // channel: [ val(meta), [ html ] ] + trim_zip // channel: [ val(meta), [ zip ] ] + trim_log // channel: [ val(meta), [ txt ] ] + trim_read_count // channel: [ val(meta), val(count) ] + + versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] +} diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml new file mode 100644 index 00000000..b05004d4 --- /dev/null +++ b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml @@ -0,0 +1,96 @@ +name: "fastq_fastqc_umitools_trimgalore" +description: Read QC, UMI extraction and trimming +keywords: + - fastq + - fastqc + - qc + - UMI + - trimming + - trimgalore +modules: + - fastqc + - umitools/extract + - trimgalore +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test' ] + - reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - skip_fastqc: + type: boolean + description: | + Skip fastqc process + - with_umi: + type: boolean + description: | + With or without umi detection + - skip_umi_extract: + type: boolean + description: | + With or without umi extrection + - skip_trimming: + type: boolean + description: | + Allows to skip trimgalore execution + - umi_discard_read: + type: integer + description: | + Discard R1 / R2 if required + - min_trimmed_reads: + type: integer + description: | + Inputs with fewer than this reads will be filtered out of the "reads" output channel + +output: + - reads: + type: file + description: > + Extracted FASTQ files. | + For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | + For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. + pattern: "*.{fastq.gz}" + - fastqc_html: + type: file + description: FastQC report + pattern: "*_{fastqc.html}" + - fastqc_zip: + type: file + description: FastQC report archive + pattern: "*_{fastqc.zip}" + - log: + type: file + description: Logfile for umi_tools + pattern: "*.{log}" + - trim_unpaired: + type: file + description: | + FastQ files containing unpaired reads from read 1 or read 2 + pattern: "*unpaired*.fq.gz" + - trim_html: + type: file + description: FastQC report (optional) + pattern: "*_{fastqc.html}" + - trim_zip: + type: file + description: FastQC report archive (optional) + pattern: "*_{fastqc.zip}" + - trim_log: + type: file + description: Trim Galore! trimming report + pattern: "*_{report.txt}" + - trim_read_count: + type: integer + description: Number of reads remaining after trimming for all input samples + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@KamilMaliszArdigen" From d67a0fda5cfadc2520e832ec3105c2bb3dc8e998 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 22:46:42 +0200 Subject: [PATCH 027/201] Install fastq_align_bwa subworkflow --- modules.json | 27 ++++++++ .../nf-core/bam_sort_stats_samtools/main.nf | 50 ++++++++++++++ .../nf-core/bam_sort_stats_samtools/meta.yml | 65 +++++++++++++++++ .../nf-core/bam_stats_samtools/main.nf | 32 +++++++++ .../nf-core/bam_stats_samtools/meta.yml | 40 +++++++++++ subworkflows/nf-core/fastq_align_bwa/main.nf | 43 ++++++++++++ subworkflows/nf-core/fastq_align_bwa/meta.yml | 69 +++++++++++++++++++ 7 files changed, 326 insertions(+) create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/main.nf create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/meta.yml create mode 100644 subworkflows/nf-core/bam_stats_samtools/main.nf create mode 100644 subworkflows/nf-core/bam_stats_samtools/meta.yml create mode 100644 subworkflows/nf-core/fastq_align_bwa/main.nf create mode 100644 subworkflows/nf-core/fastq_align_bwa/meta.yml diff --git a/modules.json b/modules.json index 8f10866d..6c75bb41 100644 --- a/modules.json +++ b/modules.json @@ -30,6 +30,7 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ + "fastq_align_bwa", "modules" ] }, @@ -179,6 +180,7 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ + "bam_stats_samtools", "modules" ] }, @@ -186,6 +188,7 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ + "bam_stats_samtools", "modules" ] }, @@ -193,6 +196,7 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ + "bam_sort_stats_samtools", "modules" ] }, @@ -200,6 +204,7 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ + "bam_sort_stats_samtools", "modules" ] }, @@ -207,6 +212,7 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ + "bam_stats_samtools", "modules" ] }, @@ -250,6 +256,27 @@ }, "subworkflows": { "nf-core": { + "bam_sort_stats_samtools": { + "branch": "master", + "git_sha": "3911652a6b24249358f79e8b8466338d63efb2a2", + "installed_by": [ + "fastq_align_bwa" + ] + }, + "bam_stats_samtools": { + "branch": "master", + "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e", + "installed_by": [ + "bam_sort_stats_samtools" + ] + }, + "fastq_align_bwa": { + "branch": "master", + "git_sha": "901fab507683647b43e7032f3ae9e4c234eb68eb", + "installed_by": [ + "subworkflows" + ] + }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "100caf3506850ffcc04953f724a97f422940c377", diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf new file mode 100644 index 00000000..617871fe --- /dev/null +++ b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf @@ -0,0 +1,50 @@ +// +// Sort, index BAM file and run samtools stats, flagstat and idxstats +// + +include { SAMTOOLS_SORT } from '../../../modules/nf-core/samtools/sort/main' +include { SAMTOOLS_INDEX } from '../../../modules/nf-core/samtools/index/main' +include { BAM_STATS_SAMTOOLS } from '../bam_stats_samtools/main' + +workflow BAM_SORT_STATS_SAMTOOLS { + take: + ch_bam // channel: [ val(meta), [ bam ] ] + ch_fasta // channel: [ fasta ] + + main: + + ch_versions = Channel.empty() + + SAMTOOLS_SORT ( ch_bam ) + ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) + + SAMTOOLS_INDEX ( SAMTOOLS_SORT.out.bam ) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) + + SAMTOOLS_SORT.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map { + meta, bam, bai, csi -> + if (bai) { + [ meta, bam, bai ] + } else { + [ meta, bam, csi ] + } + } + .set { ch_bam_bai } + + BAM_STATS_SAMTOOLS ( ch_bam_bai, ch_fasta ) + ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions) + + emit: + bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), [ csi ] ] + + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml new file mode 100644 index 00000000..131065be --- /dev/null +++ b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml @@ -0,0 +1,65 @@ +name: bam_sort_stats_samtools +description: Sort SAM/BAM/CRAM file +keywords: + - sort + - bam + - sam + - cram +modules: + - samtools/sort + - samtools/index + - samtools/stats + - samtools/idxstats + - samtools/flagstat +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - fasta: + type: file + description: Reference genome fasta file + pattern: "*.{fasta,fa}" +# TODO Update when we decide on a standard for subworkflow docs +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - bam: + type: file + description: Sorted BAM/CRAM/SAM file + pattern: "*.{bam,cram,sam}" + - bai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - crai: + type: file + description: BAM/CRAM/SAM index file + pattern: "*.{bai,crai,sai}" + - stats: + type: file + description: File containing samtools stats output + pattern: "*.{stats}" + - flagstat: + type: file + description: File containing samtools flagstat output + pattern: "*.{flagstat}" + - idxstats: + type: file + description: File containing samtools idxstats output + pattern: "*.{idxstats}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@drpatelh" + - "@ewels" diff --git a/subworkflows/nf-core/bam_stats_samtools/main.nf b/subworkflows/nf-core/bam_stats_samtools/main.nf new file mode 100644 index 00000000..c9d7c8b7 --- /dev/null +++ b/subworkflows/nf-core/bam_stats_samtools/main.nf @@ -0,0 +1,32 @@ +// +// Run SAMtools stats, flagstat and idxstats +// + +include { SAMTOOLS_STATS } from '../../../modules/nf-core/samtools/stats/main' +include { SAMTOOLS_IDXSTATS } from '../../../modules/nf-core/samtools/idxstats/main' +include { SAMTOOLS_FLAGSTAT } from '../../../modules/nf-core/samtools/flagstat/main' + +workflow BAM_STATS_SAMTOOLS { + take: + ch_bam_bai // channel: [ val(meta), path(bam), path(bai) ] + ch_fasta // channel: [ path(fasta) ] + + main: + ch_versions = Channel.empty() + + SAMTOOLS_STATS ( ch_bam_bai, ch_fasta ) + ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions) + + SAMTOOLS_FLAGSTAT ( ch_bam_bai ) + ch_versions = ch_versions.mix(SAMTOOLS_FLAGSTAT.out.versions) + + SAMTOOLS_IDXSTATS ( ch_bam_bai ) + ch_versions = ch_versions.mix(SAMTOOLS_IDXSTATS.out.versions) + + emit: + stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), path(stats) ] + flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), path(flagstat) ] + idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), path(idxstats) ] + + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/subworkflows/nf-core/bam_stats_samtools/meta.yml b/subworkflows/nf-core/bam_stats_samtools/meta.yml new file mode 100644 index 00000000..b6072686 --- /dev/null +++ b/subworkflows/nf-core/bam_stats_samtools/meta.yml @@ -0,0 +1,40 @@ +name: bam_stats_samtools +description: Produces comprehensive statistics from SAM/BAM/CRAM file +keywords: + - statistics + - counts + - bam + - sam + - cram +modules: + - samtools/stats + - samtools/idxstats + - samtools/flagstat +input: + - ch_bam_bai: + description: | + The input channel containing the BAM/CRAM and it's index + Structure: [ val(meta), path(bam), path(bai) ] + - ch_fasta: + description: | + Reference genome fasta file + Structure: [ path(fasta) ] +output: + - stats: + description: | + File containing samtools stats output + Structure: [ val(meta), path(stats) ] + - flagstat: + description: | + File containing samtools flagstat output + Structure: [ val(meta), path(flagstat) ] + - idxstats: + description: | + File containing samtools idxstats output + Structure: [ val(meta), path(idxstats)] + - versions: + description: | + Files containing software versions + Structure: [ path(versions.yml) ] +authors: + - "@drpatelh" diff --git a/subworkflows/nf-core/fastq_align_bwa/main.nf b/subworkflows/nf-core/fastq_align_bwa/main.nf new file mode 100644 index 00000000..4ce4f886 --- /dev/null +++ b/subworkflows/nf-core/fastq_align_bwa/main.nf @@ -0,0 +1,43 @@ +// +// Alignment with BWA +// + +include { BWA_MEM } from '../../../modules/nf-core/bwa/mem/main' +include { BAM_SORT_STATS_SAMTOOLS } from '../bam_sort_stats_samtools/main' + +workflow FASTQ_ALIGN_BWA { + take: + ch_reads // channel (mandatory): [ val(meta), [ path(reads) ] ] + ch_index // channel (mandatory): [ val(meta2), path(index) ] + val_sort_bam // boolean (mandatory): true or false + ch_fasta // channel (optional) : [ path(fasta) ] + + main: + ch_versions = Channel.empty() + + // + // Map reads with BWA + // + + BWA_MEM ( ch_reads, ch_index, val_sort_bam ) + ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) + + // + // Sort, index BAM file and run samtools stats, flagstat and idxstats + // + + BAM_SORT_STATS_SAMTOOLS ( BWA_MEM.out.bam, ch_fasta ) + ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) + + emit: + bam_orig = BWA_MEM.out.bam // channel: [ val(meta), path(bam) ] + + bam = BAM_SORT_STATS_SAMTOOLS.out.bam // channel: [ val(meta), path(bam) ] + bai = BAM_SORT_STATS_SAMTOOLS.out.bai // channel: [ val(meta), path(bai) ] + csi = BAM_SORT_STATS_SAMTOOLS.out.csi // channel: [ val(meta), path(csi) ] + stats = BAM_SORT_STATS_SAMTOOLS.out.stats // channel: [ val(meta), path(stats) ] + flagstat = BAM_SORT_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), path(flagstat) ] + idxstats = BAM_SORT_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), path(idxstats) ] + + versions = ch_versions // channel: [ path(versions.yml) ] +} diff --git a/subworkflows/nf-core/fastq_align_bwa/meta.yml b/subworkflows/nf-core/fastq_align_bwa/meta.yml new file mode 100644 index 00000000..03f84860 --- /dev/null +++ b/subworkflows/nf-core/fastq_align_bwa/meta.yml @@ -0,0 +1,69 @@ +name: fastq_align_bwa +description: Align reads to a reference genome using bwa then sort with samtools +keywords: + - align + - fasta + - genome + - reference +modules: + - bwa/align + - samtools/sort + - samtools/index + - samtools/stats + - samtools/idxstats + - samtools/flagstat +input: + - ch_reads: + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + Structure: [ val(meta), [ path(reads) ] ] + - ch_index: + description: | + BWA genome index files + Structure: [ val(meta2), path(index) ] + - val_sort_bam: + type: boolean + description: If true bwa modules sort resulting bam files + pattern: "true|false" + - ch_fasta: + type: file + description: | + Optional reference fasta file. This only needs to be given if val_sort_bam = true + Structure: [ path(fasta) ] + +output: + - bam_orig: + description: | + BAM file produced by bwa + Structure: [ val(meta), path(bam) ] + - bam: + description: | + BAM file ordered by samtools + Structure: [ val(meta), path(bam) ] + - bai: + description: | + BAI index of the ordered BAM file + Structure: [ val(meta), path(bai) ] + - csi: + description: | + CSI index of the ordered BAM file + Structure: [ val(meta), path(csi) ] + - stats: + description: | + File containing samtools stats output + Structure: [ val(meta), path(stats) ] + - flagstat: + description: | + File containing samtools flagstat output + Structure: [ val(meta), path(flagstat) ] + - idxstats: + description: | + File containing samtools idxstats output + Structure: [ val(meta), path(idxstats) ] + - versions: + description: | + Files containing software versions + Structure: [ path(versions.yml) ] +authors: + - "@JoseEspinosa" From 8b93ec222b5cf706ea3e682ce6e90e22b664ec26 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 22:47:29 +0200 Subject: [PATCH 028/201] Install fastq_align_bowtie2 subworkflow --- modules.json | 23 ++++--- .../nf-core/fastq_align_bowtie2/main.nf | 45 +++++++++++++ .../nf-core/fastq_align_bowtie2/meta.yml | 63 +++++++++++++++++++ 3 files changed, 124 insertions(+), 7 deletions(-) create mode 100644 subworkflows/nf-core/fastq_align_bowtie2/main.nf create mode 100644 subworkflows/nf-core/fastq_align_bowtie2/meta.yml diff --git a/modules.json b/modules.json index 6c75bb41..43a96a34 100644 --- a/modules.json +++ b/modules.json @@ -9,6 +9,7 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ + "fastq_align_bowtie2", "modules" ] }, @@ -180,16 +181,16 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ - "bam_stats_samtools", - "modules" + "modules", + "bam_stats_samtools" ] }, "samtools/idxstats": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ - "bam_stats_samtools", - "modules" + "modules", + "bam_stats_samtools" ] }, "samtools/index": { @@ -212,8 +213,8 @@ "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", "installed_by": [ - "bam_stats_samtools", - "modules" + "modules", + "bam_stats_samtools" ] }, "subread/featurecounts": { @@ -260,7 +261,8 @@ "branch": "master", "git_sha": "3911652a6b24249358f79e8b8466338d63efb2a2", "installed_by": [ - "fastq_align_bwa" + "fastq_align_bwa", + "fastq_align_bowtie2" ] }, "bam_stats_samtools": { @@ -270,6 +272,13 @@ "bam_sort_stats_samtools" ] }, + "fastq_align_bowtie2": { + "branch": "master", + "git_sha": "ac75f79157ecc64283a2b3a559f1ba90bc0f2259", + "installed_by": [ + "subworkflows" + ] + }, "fastq_align_bwa": { "branch": "master", "git_sha": "901fab507683647b43e7032f3ae9e4c234eb68eb", diff --git a/subworkflows/nf-core/fastq_align_bowtie2/main.nf b/subworkflows/nf-core/fastq_align_bowtie2/main.nf new file mode 100644 index 00000000..eaf4ac5e --- /dev/null +++ b/subworkflows/nf-core/fastq_align_bowtie2/main.nf @@ -0,0 +1,45 @@ +// +// Alignment with Bowtie2 +// + +include { BOWTIE2_ALIGN } from '../../../modules/nf-core/bowtie2/align/main' +include { BAM_SORT_STATS_SAMTOOLS } from '../bam_sort_stats_samtools/main' + +workflow FASTQ_ALIGN_BOWTIE2 { + take: + ch_reads // channel: [ val(meta), [ reads ] ] + ch_index // channel: /path/to/bowtie2/index/ + save_unaligned // val + sort_bam // val + ch_fasta // channel: /path/to/reference.fasta + + main: + + ch_versions = Channel.empty() + + // + // Map reads with Bowtie2 + // + BOWTIE2_ALIGN ( ch_reads, ch_index, save_unaligned, sort_bam ) + ch_versions = ch_versions.mix(BOWTIE2_ALIGN.out.versions.first()) + + // + // Sort, index BAM file and run samtools stats, flagstat and idxstats + // + BAM_SORT_STATS_SAMTOOLS ( BOWTIE2_ALIGN.out.bam, ch_fasta ) + ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) + + emit: + bam_orig = BOWTIE2_ALIGN.out.bam // channel: [ val(meta), bam ] + log_out = BOWTIE2_ALIGN.out.log // channel: [ val(meta), log ] + fastq = BOWTIE2_ALIGN.out.fastq // channel: [ val(meta), fastq ] + + bam = BAM_SORT_STATS_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] + bai = BAM_SORT_STATS_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] + csi = BAM_SORT_STATS_SAMTOOLS.out.csi // channel: [ val(meta), [ csi ] ] + stats = BAM_SORT_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_SORT_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_SORT_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/nf-core/fastq_align_bowtie2/meta.yml b/subworkflows/nf-core/fastq_align_bowtie2/meta.yml new file mode 100644 index 00000000..ad378077 --- /dev/null +++ b/subworkflows/nf-core/fastq_align_bowtie2/meta.yml @@ -0,0 +1,63 @@ +name: fastq_align_bowtie2 +description: Align reads to a reference genome using bowtie2 then sort with samtools +keywords: + - align + - fasta + - genome + - reference +modules: + - bowtie2/align + - samtools/sort + - samtools/index + - samtools/stats + - samtools/idxstats + - samtools/flagstat +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - ch_reads: + type: file + description: | + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - ch_index: + type: file + description: Bowtie2 genome index files + pattern: "*.ebwt" + - save_unaligned: + type: boolean + description: | + Save reads that do not map to the reference (true) or discard them (false) + (default: false) + - sort_bam: + description: | + Save reads that do not map to the reference (true) or discard them (false) + (default: false) + - ch_fasta: + type: file + description: Reference fasta file + pattern: "*.{fasta,fa}" +# TODO Update when we decide on a standard for subworkflow docs +output: + - bam: + type: file + description: Output BAM file containing read alignments + pattern: "*.{bam}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - fastq: + type: file + description: Unaligned FastQ files + pattern: "*.fastq.gz" + - log: + type: file + description: Alignment log + pattern: "*.log" +# TODO Add samtools outputs +authors: + - "@drpatelh" From 356ccd2e248860dbf14b6d986f0e603611f5a691 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 22:48:55 +0200 Subject: [PATCH 029/201] Install fastq_align_chromap subworkflow --- modules.json | 173 +++++------------- .../nf-core/fastq_align_chromap/main.nf | 41 +++++ .../nf-core/fastq_align_chromap/meta.yml | 99 ++++++++++ 3 files changed, 185 insertions(+), 128 deletions(-) create mode 100644 subworkflows/nf-core/fastq_align_chromap/main.nf create mode 100644 subworkflows/nf-core/fastq_align_chromap/meta.yml diff --git a/modules.json b/modules.json index 43a96a34..d7abe7b4 100644 --- a/modules.json +++ b/modules.json @@ -8,250 +8,173 @@ "bowtie2/align": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "fastq_align_bowtie2", - "modules" - ] + "installed_by": ["fastq_align_bowtie2", "modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "fastq_align_bwa", - "modules" - ] + "installed_by": ["fastq_align_bwa", "modules"] }, "chromap/chromap": { "branch": "master", "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", - "installed_by": [ - "modules" - ] + "installed_by": ["fastq_align_chromap", "modules"] }, "chromap/index": { "branch": "master", "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/getchromsizes": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ], + "installed_by": ["modules"], "patch": "modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff" }, "deeptools/computematrix": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": [ - "modules", - "fastq_fastqc_umitools_trimgalore" - ] + "installed_by": ["modules", "fastq_fastqc_umitools_trimgalore"] }, "gffread": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "macs2/callpeak": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "phantompeakqualtools": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/markduplicates": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "preseq/lcextrap": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules", - "bam_stats_samtools" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/idxstats": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules", - "bam_stats_samtools" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/index": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_sort_stats_samtools", "modules"] }, "samtools/sort": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_sort_stats_samtools", "modules"] }, "samtools/stats": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules", - "bam_stats_samtools" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "subread/featurecounts": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "trimgalore": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules", - "fastq_fastqc_umitools_trimgalore" - ] + "installed_by": ["modules", "fastq_fastqc_umitools_trimgalore"] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "umitools/extract": { "branch": "master", "git_sha": "ffe2f5865f608848e440a52b73c304ea79aaf818", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore"] }, "untar": { "branch": "master", "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -260,41 +183,35 @@ "bam_sort_stats_samtools": { "branch": "master", "git_sha": "3911652a6b24249358f79e8b8466338d63efb2a2", - "installed_by": [ - "fastq_align_bwa", - "fastq_align_bowtie2" - ] + "installed_by": ["fastq_align_bowtie2", "fastq_align_chromap", "fastq_align_bwa"] }, "bam_stats_samtools": { "branch": "master", "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e", - "installed_by": [ - "bam_sort_stats_samtools" - ] + "installed_by": ["bam_sort_stats_samtools"] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "ac75f79157ecc64283a2b3a559f1ba90bc0f2259", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_bwa": { "branch": "master", "git_sha": "901fab507683647b43e7032f3ae9e4c234eb68eb", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] + }, + "fastq_align_chromap": { + "branch": "master", + "git_sha": "ac75f79157ecc64283a2b3a559f1ba90bc0f2259", + "installed_by": ["subworkflows"] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "100caf3506850ffcc04953f724a97f422940c377", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/fastq_align_chromap/main.nf b/subworkflows/nf-core/fastq_align_chromap/main.nf new file mode 100644 index 00000000..9d706c98 --- /dev/null +++ b/subworkflows/nf-core/fastq_align_chromap/main.nf @@ -0,0 +1,41 @@ +/* + * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats + */ + +include { CHROMAP_CHROMAP } from '../../../modules/nf-core/chromap/chromap/main' +include { BAM_SORT_STATS_SAMTOOLS } from '../bam_sort_stats_samtools/main' + +workflow FASTQ_ALIGN_CHROMAP { + take: + ch_reads // channel (mandatory): [ val(meta), [ reads ] ] + ch_index // channel (mandatory): [ val(meta2, [ index ] ] + ch_fasta // channel (mandatory): [ val(meta2, [ fasta ] ] + ch_barcodes // channel (optional): [ barcodes ] + ch_whitelist // channel (optional): [ whitelist ] + ch_chr_order // channel (optional): [ chr_order ] + ch_pairs_chr_order // channel (optional): [ pairs_chr_order ] + + main: + ch_versions = Channel.empty() + + // + // Map reads with CHROMAP + // + CHROMAP_CHROMAP(ch_reads, ch_fasta, ch_index, ch_barcodes, ch_whitelist, ch_chr_order, ch_pairs_chr_order) + ch_versions = ch_versions.mix(CHROMAP_CHROMAP.out.versions) + + // + // Sort, index BAM file and run samtools stats, flagstat and idxstats + // + BAM_SORT_STATS_SAMTOOLS(CHROMAP_CHROMAP.out.bam, ch_fasta.map { it[1] }) + ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) + + emit: + bam = BAM_SORT_STATS_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] + bai = BAM_SORT_STATS_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] + stats = BAM_SORT_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_SORT_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_SORT_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + + versions = ch_versions // path: versions.yml +} diff --git a/subworkflows/nf-core/fastq_align_chromap/meta.yml b/subworkflows/nf-core/fastq_align_chromap/meta.yml new file mode 100644 index 00000000..6d701bce --- /dev/null +++ b/subworkflows/nf-core/fastq_align_chromap/meta.yml @@ -0,0 +1,99 @@ +name: "fastq_align_chromap" +description: Align high throughput chromatin profiles using Chromap then sort with samtools +keywords: + - align + - fasta + - genome + - reference + - chromatin profiles + - chip-seq + - atac-seq + - hic +modules: + - chromap/chromap + - samtools/sort + - samtools/index + - samtools/stats + - samtools/idxstats + - samtools/flagstat +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - ch_reads: + type: file + description: | + Structure: [val(meta), path(reads)] + List of input FastQ files of size 1 and 2 for single-end and paired-end data, + respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] + - ch_index: + type: file + description: | + Structure: [val(meta2), path(index)] + Chromap genome index files + pattern: "*.index" + - ch_fasta: + type: file + description: | + Structure: [val(meta2), path(fasta)] + Reference fasta file + pattern: "*.{fasta,fa}" + - ch_barcodes: + type: file + description: | + Structure: [path(barcodes)] + Cell barcode files + - ch_whitelist: + type: file + description: | + Structure: [path(whitelist)] + Cell barcode whitelist file + - ch_chr_order: + type: file + description: | + Structure: [path(chr_order)] + Custom chromosome order + - ch_pairs_chr_order: + type: file + description: | + Structure: [path(pairs_chr_order)] + Natural chromosome order for pairs flipping +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test' ] + - bam: + type: file + description: BAM file + pattern: "*.bam" + - bai: + type: file + description: BAM index (currently only for snapaligner) + pattern: "*.bai" + - stats: + type: file + description: File containing samtools stats output + pattern: "*.{stats}" + - flagstat: + type: file + description: File containing samtools flagstat output + pattern: "*.{flagstat}" + - idxstats: + type: file + description: File containing samtools idxstats output + pattern: "*.{idxstats}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@JoseEspinosa" From c8739edbebf1c3cdca696a50653f712f1817da6d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 23:08:50 +0200 Subject: [PATCH 030/201] use the FASTQ_FASTQC_UMITOOLS_TRIMGALORE subworkflow insead of fastq_trimgalore --- conf/modules.config | 18 ++++++--- subworkflows/nf-core/fastqc_trimgalore.nf | 48 ----------------------- workflows/chipseq.nf | 26 ++++++------ 3 files changed, 27 insertions(+), 65 deletions(-) delete mode 100644 subworkflows/nf-core/fastqc_trimgalore.nf diff --git a/conf/modules.config b/conf/modules.config index b46fec83..f4c55854 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -122,7 +122,7 @@ process { if (!(params.skip_fastqc || params.skip_qc)) { process { - withName: '.*:FASTQC_TRIMGALORE:FASTQC' { + withName: 'FASTQC' { ext.args = '--quiet' publishDir = [ [ @@ -142,11 +142,17 @@ if (!(params.skip_fastqc || params.skip_qc)) { if (!params.skip_trimming) { process { - withName: '.*:FASTQC_TRIMGALORE:TRIMGALORE' { - ext.args = [ - '--fastqc', - params.trim_nextseq > 0 ? "--nextseq ${params.trim_nextseq}" : '' - ].join(' ').trim() + withName: 'TRIMGALORE' { + ext.args = { + [ + '--fastqc', + params.trim_nextseq > 0 ? "--nextseq ${params.trim_nextseq}" : '', + params.clip_r1 > 0 ? "--clip_r1 ${params.clip_r1}" : '', + params.clip_r2 > 0 ? "--clip_r2 ${params.clip_r2}" : '', + params.three_prime_clip_r1 > 0 ? "--three_prime_clip_r1 ${params.three_prime_clip_r1}" : '', + params.three_prime_clip_r2 > 0 ? "--three_prime_clip_r2 ${params.three_prime_clip_r2}" : '' + ].join(' ').trim() + } publishDir = [ [ path: { "${params.outdir}/trimgalore/fastqc" }, diff --git a/subworkflows/nf-core/fastqc_trimgalore.nf b/subworkflows/nf-core/fastqc_trimgalore.nf deleted file mode 100644 index 8a18098a..00000000 --- a/subworkflows/nf-core/fastqc_trimgalore.nf +++ /dev/null @@ -1,48 +0,0 @@ -// -// Read QC and trimming -// - -include { FASTQC } from '../../modules/nf-core/fastqc/main' -include { TRIMGALORE } from '../../modules/nf-core/trimgalore/main' - -workflow FASTQC_TRIMGALORE { - take: - reads // channel: [ val(meta), [ reads ] ] - skip_fastqc // boolean: true/false - skip_trimming // boolean: true/false - - main: - - ch_versions = Channel.empty() - fastqc_html = Channel.empty() - fastqc_zip = Channel.empty() - if (!skip_fastqc) { - FASTQC ( reads ).html.set { fastqc_html } - fastqc_zip = FASTQC.out.zip - ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - } - - trim_reads = reads - trim_html = Channel.empty() - trim_zip = Channel.empty() - trim_log = Channel.empty() - if (!skip_trimming) { - TRIMGALORE ( reads ).reads.set { trim_reads } - trim_html = TRIMGALORE.out.html - trim_zip = TRIMGALORE.out.zip - trim_log = TRIMGALORE.out.log - ch_versions = ch_versions.mix(TRIMGALORE.out.versions.first()) - } - - emit: - reads = trim_reads // channel: [ val(meta), [ reads ] ] - - fastqc_html // channel: [ val(meta), [ html ] ] - fastqc_zip // channel: [ val(meta), [ zip ] ] - - trim_html // channel: [ val(meta), [ html ] ] - trim_zip // channel: [ val(meta), [ zip ] ] - trim_log // channel: [ val(meta), [ txt ] ] - - versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] -} diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index caaa4c0c..d2279680 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -115,7 +115,7 @@ include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../module // SUBWORKFLOW: Consisting entirely of nf-core/modules // -include { FASTQC_TRIMGALORE } from '../subworkflows/nf-core/fastqc_trimgalore' +include { FASTQ_FASTQC_UMITOOLS_TRIMGALORE } from '../subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main' include { ALIGN_BWA_MEM } from '../subworkflows/nf-core/align_bwa_mem' include { ALIGN_BOWTIE2 } from '../subworkflows/nf-core/align_bowtie2' include { ALIGN_CHROMAP } from '../subworkflows/nf-core/align_chromap' @@ -155,12 +155,16 @@ workflow CHIPSEQ { // // SUBWORKFLOW: Read QC and trim adapters // - FASTQC_TRIMGALORE ( + FASTQ_FASTQC_UMITOOLS_TRIMGALORE ( INPUT_CHECK.out.reads, params.skip_fastqc || params.skip_qc, - params.skip_trimming + false, + false, + params.skip_trimming, + 0, + 10000 ) - ch_versions = ch_versions.mix(FASTQC_TRIMGALORE.out.versions) + ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.versions) // // SUBWORKFLOW: Alignment with BWA & BAM QC @@ -172,7 +176,7 @@ workflow CHIPSEQ { ch_samtools_idxstats = Channel.empty() if (params.aligner == 'bwa') { ALIGN_BWA_MEM ( - FASTQC_TRIMGALORE.out.reads, + FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.bwa_index ) ch_genome_bam = ALIGN_BWA_MEM.out.bam @@ -188,7 +192,7 @@ workflow CHIPSEQ { // if (params.aligner == 'bowtie2') { ALIGN_BOWTIE2 ( - FASTQC_TRIMGALORE.out.reads, + FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.bowtie2_index, params.save_unaligned ) @@ -205,7 +209,7 @@ workflow CHIPSEQ { // if (params.aligner == 'chromap') { ALIGN_CHROMAP ( - FASTQC_TRIMGALORE.out.reads, + FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.chromap_index, PREPARE_GENOME.out.fasta .map { @@ -225,7 +229,7 @@ workflow CHIPSEQ { // if (params.aligner == 'star') { ALIGN_STAR ( - FASTQC_TRIMGALORE.out.reads, + FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.star_index ) ch_genome_bam = ALIGN_STAR.out.bam @@ -675,9 +679,9 @@ workflow CHIPSEQ { ch_methods_description.collectFile(name: 'methods_description_mqc.yaml'), ch_multiqc_logo.collect().ifEmpty([]), - FASTQC_TRIMGALORE.out.fastqc_zip.collect{it[1]}.ifEmpty([]), - FASTQC_TRIMGALORE.out.trim_zip.collect{it[1]}.ifEmpty([]), - FASTQC_TRIMGALORE.out.trim_log.collect{it[1]}.ifEmpty([]), + FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.fastqc_zip.collect{it[1]}.ifEmpty([]), + FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.trim_zip.collect{it[1]}.ifEmpty([]), + FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.trim_log.collect{it[1]}.ifEmpty([]), ch_samtools_stats.collect{it[1]}.ifEmpty([]), ch_samtools_flagstat.collect{it[1]}.ifEmpty([]), From 8af9cb2991f30890ba1d1bd7cbb3cce9e690b717 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 17 Apr 2023 23:16:23 +0200 Subject: [PATCH 031/201] Use FASTQ_ALIGN_BWA subworkflow --- conf/modules.config | 12 +++++---- subworkflows/nf-core/align_bwa_mem.nf | 37 --------------------------- workflows/chipseq.nf | 21 ++++++++------- 3 files changed, 19 insertions(+), 51 deletions(-) delete mode 100644 subworkflows/nf-core/align_bwa_mem.nf diff --git a/conf/modules.config b/conf/modules.config index f4c55854..01316ea6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -214,11 +214,13 @@ process { if (params.aligner == 'bwa') { process { withName: 'BWA_MEM' { - ext.args = { [ - '-M', - params.bwa_min_score ? " -T ${params.bwa_min_score}" : '', - meta.read_group ? "-R ${meta.read_group}": '' - ].join(' ').trim() } + ext.args = { + [ + '-M', + params.bwa_min_score ? " -T ${params.bwa_min_score}" : '', + meta.read_group ? "-R ${meta.read_group}": '' + ].join(' ').trim() + } ext.args2 = '-bhS -F 0x0100 -O BAM' ext.prefix = { "${meta.id}.Lb" } publishDir = [ diff --git a/subworkflows/nf-core/align_bwa_mem.nf b/subworkflows/nf-core/align_bwa_mem.nf deleted file mode 100644 index eac98188..00000000 --- a/subworkflows/nf-core/align_bwa_mem.nf +++ /dev/null @@ -1,37 +0,0 @@ -/* - * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats - */ - -include { BWA_MEM } from '../../modules/nf-core/bwa/mem/main' -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' - -workflow ALIGN_BWA_MEM { - take: - reads // channel: [ val(meta), [ reads ] ] - index // path: /path/to/index - - main: - - ch_versions = Channel.empty() - - // - // Map reads with BWA - // - BWA_MEM(reads, index, false) - ch_versions = ch_versions.mix(BWA_MEM.out.versions.first()) - - // - // Sort, index BAM file and run samtools stats, flagstat and idxstats - // - BAM_SORT_SAMTOOLS(BWA_MEM.out.bam) - ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions.first()) - - emit: - bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] - bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] - stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // path: versions.yml -} diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index d2279680..dfd2df76 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -116,7 +116,8 @@ include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../module // include { FASTQ_FASTQC_UMITOOLS_TRIMGALORE } from '../subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main' -include { ALIGN_BWA_MEM } from '../subworkflows/nf-core/align_bwa_mem' +include { FASTQ_ALIGN_BWA } from '../subworkflows/nf-core/fastq_align_bwa/main' + include { ALIGN_BOWTIE2 } from '../subworkflows/nf-core/align_bowtie2' include { ALIGN_CHROMAP } from '../subworkflows/nf-core/align_chromap' include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' @@ -175,16 +176,18 @@ workflow CHIPSEQ { ch_samtools_flagstat = Channel.empty() ch_samtools_idxstats = Channel.empty() if (params.aligner == 'bwa') { - ALIGN_BWA_MEM ( + FASTQ_ALIGN_BWA ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, - PREPARE_GENOME.out.bwa_index + PREPARE_GENOME.out.bwa_index, + false, + PREPARE_GENOME.out.fasta ) - ch_genome_bam = ALIGN_BWA_MEM.out.bam - ch_genome_bam_index = ALIGN_BWA_MEM.out.bai - ch_samtools_stats = ALIGN_BWA_MEM.out.stats - ch_samtools_flagstat = ALIGN_BWA_MEM.out.flagstat - ch_samtools_idxstats = ALIGN_BWA_MEM.out.idxstats - ch_versions = ch_versions.mix(ALIGN_BWA_MEM.out.versions.first()) + ch_genome_bam = FASTQ_ALIGN_BWA.out.bam + ch_genome_bam_index = FASTQ_ALIGN_BWA.out.bai + ch_samtools_stats = FASTQ_ALIGN_BWA.out.stats + ch_samtools_flagstat = FASTQ_ALIGN_BWA.out.flagstat + ch_samtools_idxstats = FASTQ_ALIGN_BWA.out.idxstats + ch_versions = ch_versions.mix(FASTQ_ALIGN_BWA.out.versions) } // From d7f074a63793bcb3ad9b51af0ee2bc7511a3159c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 10:25:46 +0200 Subject: [PATCH 032/201] Use FASTQ_ALIGN_BOWTIE2 subworkflow --- subworkflows/nf-core/align_bowtie2.nf | 38 --------------------------- workflows/chipseq.nf | 20 +++++++------- 2 files changed, 11 insertions(+), 47 deletions(-) delete mode 100644 subworkflows/nf-core/align_bowtie2.nf diff --git a/subworkflows/nf-core/align_bowtie2.nf b/subworkflows/nf-core/align_bowtie2.nf deleted file mode 100644 index 4661c720..00000000 --- a/subworkflows/nf-core/align_bowtie2.nf +++ /dev/null @@ -1,38 +0,0 @@ -/* - * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats - */ - -include { BOWTIE2_ALIGN } from '../../modules/nf-core/bowtie2/align/main' -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' - -workflow ALIGN_BOWTIE2 { - take: - reads // channel: [ val(meta), [ reads ] ] - index // path: /path/to/index - save_unaligned // boolean: true/false - - main: - - ch_versions = Channel.empty() - - // - // Map reads with BWA - // - BOWTIE2_ALIGN(reads, index, save_unaligned, false) - ch_versions = ch_versions.mix(BOWTIE2_ALIGN.out.versions.first()) - - // - // Sort, index BAM file and run samtools stats, flagstat and idxstats - // - BAM_SORT_SAMTOOLS(BOWTIE2_ALIGN.out.bam) - ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions.first()) - - emit: - bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] - bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] - stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // path: versions.yml -} diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index dfd2df76..45de3811 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -117,8 +117,8 @@ include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../module include { FASTQ_FASTQC_UMITOOLS_TRIMGALORE } from '../subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main' include { FASTQ_ALIGN_BWA } from '../subworkflows/nf-core/fastq_align_bwa/main' +include { FASTQ_ALIGN_BOWTIE2 } from '../subworkflows/nf-core/fastq_align_bowtie2/main' -include { ALIGN_BOWTIE2 } from '../subworkflows/nf-core/align_bowtie2' include { ALIGN_CHROMAP } from '../subworkflows/nf-core/align_chromap' include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' @@ -194,17 +194,19 @@ workflow CHIPSEQ { // SUBWORKFLOW: Alignment with Bowtie2 & BAM QC // if (params.aligner == 'bowtie2') { - ALIGN_BOWTIE2 ( + FASTQ_ALIGN_BOWTIE2 ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.bowtie2_index, - params.save_unaligned + params.save_unaligned, + false, + PREPARE_GENOME.out.fasta ) - ch_genome_bam = ALIGN_BOWTIE2.out.bam - ch_genome_bam_index = ALIGN_BOWTIE2.out.bai - ch_samtools_stats = ALIGN_BOWTIE2.out.stats - ch_samtools_flagstat = ALIGN_BOWTIE2.out.flagstat - ch_samtools_idxstats = ALIGN_BOWTIE2.out.idxstats - ch_versions = ch_versions.mix(ALIGN_BOWTIE2.out.versions.first()) + ch_genome_bam = FASTQ_ALIGN_BOWTIE2.out.bam + ch_genome_bam_index = FASTQ_ALIGN_BOWTIE2.out.bai + ch_samtools_stats = FASTQ_ALIGN_BOWTIE2.out.stats + ch_samtools_flagstat = FASTQ_ALIGN_BOWTIE2.out.flagstat + ch_samtools_idxstats = FASTQ_ALIGN_BOWTIE2.out.idxstats + ch_versions = ch_versions.mix(FASTQ_ALIGN_BOWTIE2.out.versions.first()) } // From 28de291ad88fc1c6c8fac99932f9558ae22174b9 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 16:01:06 +0200 Subject: [PATCH 033/201] Install custom/getchromsizes and picard/collectmultiplemetrics --- modules.json | 7 +++--- .../getchromsizes/custom-getchromsizes.diff | 23 ------------------- modules/nf-core/custom/getchromsizes/main.nf | 15 ++++++------ .../picard/collectmultiplemetrics/main.nf | 20 ++++++++-------- .../picard/collectmultiplemetrics/meta.yml | 18 +++++++++++++-- 5 files changed, 37 insertions(+), 46 deletions(-) delete mode 100644 modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff diff --git a/modules.json b/modules.json index d7abe7b4..22fd3128 100644 --- a/modules.json +++ b/modules.json @@ -42,9 +42,8 @@ }, "custom/getchromsizes": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["modules"], - "patch": "modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff" + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "installed_by": ["modules"] }, "deeptools/computematrix": { "branch": "master", @@ -108,7 +107,7 @@ }, "picard/collectmultiplemetrics": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "75027bf77472b1f4fd2cdd7e46f83119dfb0f2c6", "installed_by": ["modules"] }, "picard/markduplicates": { diff --git a/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff b/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff deleted file mode 100644 index 18fda554..00000000 --- a/modules/nf-core/custom/getchromsizes/custom-getchromsizes.diff +++ /dev/null @@ -1,23 +0,0 @@ -Changes in module 'nf-core/custom/getchromsizes' ---- modules/nf-core/custom/getchromsizes/main.nf -+++ modules/nf-core/custom/getchromsizes/main.nf -@@ -8,13 +8,12 @@ - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" - - input: -- tuple val(meta), path(fasta) -+ path(fasta) - - output: -- tuple val(meta), path ("*.sizes"), emit: sizes -- tuple val(meta), path ("*.fai") , emit: fai -- tuple val(meta), path ("*.gzi") , emit: gzi, optional: true -- path "versions.yml" , emit: versions -+ path '*.sizes' , emit: sizes -+ path '*.fai' , emit: fai -+ path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - -************************************************************ diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf index 562c693e..580f87fe 100644 --- a/modules/nf-core/custom/getchromsizes/main.nf +++ b/modules/nf-core/custom/getchromsizes/main.nf @@ -2,18 +2,19 @@ process CUSTOM_GETCHROMSIZES { tag "$fasta" label 'process_single' - conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : + 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: - path(fasta) + tuple val(meta), path(fasta) output: - path '*.sizes' , emit: sizes - path '*.fai' , emit: fai - path "versions.yml", emit: versions + tuple val(meta), path ("*.sizes"), emit: sizes + tuple val(meta), path ("*.fai") , emit: fai + tuple val(meta), path ("*.gzi") , emit: gzi, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/picard/collectmultiplemetrics/main.nf b/modules/nf-core/picard/collectmultiplemetrics/main.nf index 63f4e872..ed88dbe7 100644 --- a/modules/nf-core/picard/collectmultiplemetrics/main.nf +++ b/modules/nf-core/picard/collectmultiplemetrics/main.nf @@ -1,16 +1,16 @@ process PICARD_COLLECTMULTIPLEMETRICS { tag "$meta.id" - label 'process_medium' + label 'process_single' - conda (params.enable_conda ? "bioconda::picard=2.27.4" : null) + conda "bioconda::picard=3.0.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:2.27.4--hdfd78af_0' : - 'quay.io/biocontainers/picard:2.27.4--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : + 'quay.io/biocontainers/picard:3.0.0--hdfd78af_1' }" input: - tuple val(meta), path(bam) - path fasta - path fai + tuple val(meta) , path(bam), path(bai) + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) output: tuple val(meta), path("*_metrics"), emit: metrics @@ -24,15 +24,15 @@ process PICARD_COLLECTMULTIPLEMETRICS { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def reference = fasta ? "--REFERENCE_SEQUENCE ${fasta}" : "" - def avail_mem = 3 + def avail_mem = 3072 if (!task.memory) { log.info '[Picard CollectMultipleMetrics] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { - avail_mem = task.memory.giga + avail_mem = (task.memory.mega*0.8).intValue() } """ picard \\ - -Xmx${avail_mem}g \\ + -Xmx${avail_mem}M \\ CollectMultipleMetrics \\ $args \\ --INPUT $bam \\ diff --git a/modules/nf-core/picard/collectmultiplemetrics/meta.yml b/modules/nf-core/picard/collectmultiplemetrics/meta.yml index c11b02cf..22656080 100644 --- a/modules/nf-core/picard/collectmultiplemetrics/meta.yml +++ b/modules/nf-core/picard/collectmultiplemetrics/meta.yml @@ -23,11 +23,25 @@ input: e.g. [ id:'test', single_end:false ] - bam: type: file - description: BAM file - pattern: "*.{bam}" + description: SAM/BAM/CRAM file + pattern: "*.{sam,bam,cram}" + - bai: + type: file + description: Optional SAM/BAM/CRAM file index + pattern: "*.{sai,bai,crai}" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome'] - fasta: type: file description: Genome fasta file + - meta3: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome'] - fai: type: file description: Index of FASTA file. Only needed when fasta is supplied. From 4c462b3134dfb423e983be69a94180218edea8c7 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 16:07:24 +0200 Subject: [PATCH 034/201] Remove substituted modules and subworkflows --- modules/local/bam_filter.nf | 47 ----------------------- subworkflows/local/filter_bam_bamtools.nf | 36 ----------------- subworkflows/nf-core/align_chromap.nf | 38 ------------------ subworkflows/nf-core/align_star.nf | 46 ---------------------- subworkflows/nf-core/bam_sort_samtools.nf | 34 ---------------- 5 files changed, 201 deletions(-) delete mode 100644 modules/local/bam_filter.nf delete mode 100644 subworkflows/local/filter_bam_bamtools.nf delete mode 100644 subworkflows/nf-core/align_chromap.nf delete mode 100644 subworkflows/nf-core/align_star.nf delete mode 100644 subworkflows/nf-core/bam_sort_samtools.nf diff --git a/modules/local/bam_filter.nf b/modules/local/bam_filter.nf deleted file mode 100644 index ff07c084..00000000 --- a/modules/local/bam_filter.nf +++ /dev/null @@ -1,47 +0,0 @@ -/* - * Filter BAM file - */ -process BAM_FILTER { - tag "$meta.id" - label 'process_medium' - - conda (params.enable_conda ? "bioconda::bamtools=2.5.2 bioconda::samtools=1.15.1" : null) - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' : - 'quay.io/biocontainers/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' }" - - input: - tuple val(meta), path(bam), path(bai) - path bed - path bamtools_filter_se_config - path bamtools_filter_pe_config - - output: - tuple val(meta), path("*.bam"), emit: bam - path "versions.yml" , emit: versions - - script: - def prefix = task.ext.prefix ?: "${meta.id}" - def filter_params = meta.single_end ? '-F 0x004' : '-F 0x004 -F 0x0008 -f 0x001' - def dup_params = params.keep_dups ? '' : '-F 0x0400' - def multimap_params = params.keep_multi_map ? '' : '-q 1' - def blacklist_params = params.blacklist ? "-L $bed" : '' - def config = meta.single_end ? bamtools_filter_se_config : bamtools_filter_pe_config - """ - samtools view \\ - $filter_params \\ - $dup_params \\ - $multimap_params \\ - $blacklist_params \\ - -b $bam \\ - | bamtools filter \\ - -out ${prefix}.bam \\ - -script $config - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') - bamtools: \$(echo \$(bamtools --version 2>&1) | sed 's/^.*bamtools //; s/Part .*\$//') - END_VERSIONS - """ -} diff --git a/subworkflows/local/filter_bam_bamtools.nf b/subworkflows/local/filter_bam_bamtools.nf deleted file mode 100644 index 40e9b1be..00000000 --- a/subworkflows/local/filter_bam_bamtools.nf +++ /dev/null @@ -1,36 +0,0 @@ -/* - * Filter BAM file - */ - -include { BAM_FILTER } from '../../modules/local/bam_filter' -include { BAM_REMOVE_ORPHANS } from '../../modules/local/bam_remove_orphans' -include { BAM_SORT_SAMTOOLS } from '../nf-core/bam_sort_samtools' - -workflow FILTER_BAM_BAMTOOLS { - take: - ch_bam_bai // channel: [ val(meta), [ bam ], [bai] ] - ch_bed // channel: [ bed ] - bamtools_filter_se_config // file: BAMtools filter JSON config file for SE data - bamtools_filter_pe_config // file: BAMtools filter JSON config file for PE data - - main: - ch_versions = Channel.empty() - - BAM_FILTER(ch_bam_bai, ch_bed, bamtools_filter_se_config, bamtools_filter_pe_config) - BAM_REMOVE_ORPHANS(BAM_FILTER.out.bam) - BAM_SORT_SAMTOOLS(BAM_REMOVE_ORPHANS.out.bam) - - ch_versions = ch_versions.mix(BAM_FILTER.out.versions, - BAM_REMOVE_ORPHANS.out.versions, - BAM_SORT_SAMTOOLS.out.versions) - - emit: - name_bam = BAM_REMOVE_ORPHANS.out.bam // channel: [ val(meta), [ bam ] ] - bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] - bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] - stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/nf-core/align_chromap.nf b/subworkflows/nf-core/align_chromap.nf deleted file mode 100644 index 29b77442..00000000 --- a/subworkflows/nf-core/align_chromap.nf +++ /dev/null @@ -1,38 +0,0 @@ -/* - * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats - */ - -include { CHROMAP_CHROMAP } from '../../modules/nf-core/chromap/chromap/main' -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' - -workflow ALIGN_CHROMAP { - take: - reads // channel: [ val(meta), [ reads ] ] - index // path: /path/to/index - fasta // path: /path/to/fasta - - main: - - ch_versions = Channel.empty() - - // - // Map reads with CHROMAP - // - CHROMAP_CHROMAP(reads, fasta, index, [], [], [], []) - ch_versions = ch_versions.mix(CHROMAP_CHROMAP.out.versions.first()) - - // - // Sort, index BAM file and run samtools stats, flagstat and idxstats - // - BAM_SORT_SAMTOOLS(CHROMAP_CHROMAP.out.bam) - ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions.first()) - - emit: - bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] - bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] - stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // path: versions.yml -} diff --git a/subworkflows/nf-core/align_star.nf b/subworkflows/nf-core/align_star.nf deleted file mode 100644 index 47462182..00000000 --- a/subworkflows/nf-core/align_star.nf +++ /dev/null @@ -1,46 +0,0 @@ -/* - * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats - */ - -include { STAR_ALIGN } from '../../modules/local/star_align' -include { BAM_SORT_SAMTOOLS } from './bam_sort_samtools' - -workflow ALIGN_STAR { - take: - reads // channel: [ val(meta), [ reads ] ] - index // channel: /path/to/star/index/ - - main: - - ch_versions = Channel.empty() - - // - // Map reads with STAR - // - STAR_ALIGN ( reads, index ) - ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first()) - - // - // Sort, index BAM file and run samtools stats, flagstat and idxstats - // - BAM_SORT_SAMTOOLS ( STAR_ALIGN.out.bam ) - ch_versions = ch_versions.mix(BAM_SORT_SAMTOOLS.out.versions) - - emit: - orig_bam = STAR_ALIGN.out.bam // channel: [ val(meta), bam ] - log_final = STAR_ALIGN.out.log_final // channel: [ val(meta), log_final ] - log_out = STAR_ALIGN.out.log_out // channel: [ val(meta), log_out ] - log_progress = STAR_ALIGN.out.log_progress // channel: [ val(meta), log_progress ] - bam_sorted = STAR_ALIGN.out.bam_sorted // channel: [ val(meta), bam_sorted ] - bam_transcript = STAR_ALIGN.out.bam_transcript // channel: [ val(meta), bam_transcript ] - fastq = STAR_ALIGN.out.fastq // channel: [ val(meta), fastq ] - tab = STAR_ALIGN.out.tab // channel: [ val(meta), tab ] - - bam = BAM_SORT_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] - bai = BAM_SORT_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] - stats = BAM_SORT_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_SORT_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_SORT_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/subworkflows/nf-core/bam_sort_samtools.nf b/subworkflows/nf-core/bam_sort_samtools.nf deleted file mode 100644 index deb06c4d..00000000 --- a/subworkflows/nf-core/bam_sort_samtools.nf +++ /dev/null @@ -1,34 +0,0 @@ -/* - * Sort, index BAM file and run samtools stats, flagstat and idxstats - */ - -include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' -include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' - -workflow BAM_SORT_SAMTOOLS { - take: - ch_bam // channel: [ val(meta), [ bam ] ] - - main: - - ch_versions = Channel.empty() - - SAMTOOLS_SORT(ch_bam) - ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) - - SAMTOOLS_INDEX(SAMTOOLS_SORT.out.bam) - ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) - - BAM_STATS_SAMTOOLS(SAMTOOLS_SORT.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])) - ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions) - - emit: - bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] - bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] - stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // channel: [ versions.yml ] -} From 3a33ed9119b89cfa4eceb0020413656eeb8e5f31 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 16:08:31 +0200 Subject: [PATCH 035/201] Rename bamtools/filter --- modules/local/bamtools_filter.nf | 43 ++++++++++++++++++++++++++++++++ 1 file changed, 43 insertions(+) create mode 100644 modules/local/bamtools_filter.nf diff --git a/modules/local/bamtools_filter.nf b/modules/local/bamtools_filter.nf new file mode 100644 index 00000000..ee469ffd --- /dev/null +++ b/modules/local/bamtools_filter.nf @@ -0,0 +1,43 @@ +process BAMTOOLS_FILTER { + tag "$meta.id" + label 'process_medium' + + conda (params.enable_conda ? "bioconda::bamtools=2.5.2 bioconda::samtools=1.15.1" : null) + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' : + 'quay.io/biocontainers/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' }" + + input: + tuple val(meta), path(bam), path(bai) + path bed + path bamtools_filter_se_config + path bamtools_filter_pe_config + + output: + tuple val(meta), path("*.bam"), emit: bam + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def blacklist = bed ? "-L $bed" : '' + def config = meta.single_end ? bamtools_filter_se_config : bamtools_filter_pe_config + """ + samtools view \\ + $args \\ + $blacklist \\ + -b $bam \\ + | bamtools filter \\ + -out ${prefix}.bam \\ + -script $config + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + bamtools: \$(echo \$(bamtools --version 2>&1) | sed 's/^.*bamtools //; s/Part .*\$//') + END_VERSIONS + """ +} From 0f70b6d380d9801eeae999c9f0d2bbc03f6b6dec Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 16:09:37 +0200 Subject: [PATCH 036/201] add align_star local subworkflow --- subworkflows/local/align_star.nf | 48 ++++++++++++++++++++++++++++++++ 1 file changed, 48 insertions(+) create mode 100644 subworkflows/local/align_star.nf diff --git a/subworkflows/local/align_star.nf b/subworkflows/local/align_star.nf new file mode 100644 index 00000000..843f303e --- /dev/null +++ b/subworkflows/local/align_star.nf @@ -0,0 +1,48 @@ +/* + * Map reads, sort, index BAM file and run samtools stats, flagstat and idxstats + */ + +include { STAR_ALIGN } from '../../modules/local/star_align' +include { BAM_SORT_STATS_SAMTOOLS } from '../nf-core/bam_sort_stats_samtools/main' + +workflow ALIGN_STAR { + take: + ch_reads // channel: [ val(meta), [ reads ] ] + ch_index // channel: /path/to/star/index/ + ch_fasta // channel: /path/to/fasta + seq_center // string: sequencing center + + main: + + ch_versions = Channel.empty() + + // + // Map reads with STAR + // + STAR_ALIGN ( ch_reads, ch_index, seq_center ) + ch_versions = ch_versions.mix(STAR_ALIGN.out.versions.first()) + + // + // Sort, index BAM file and run samtools stats, flagstat and idxstats + // + BAM_SORT_STATS_SAMTOOLS ( STAR_ALIGN.out.bam, ch_fasta ) + ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) + + emit: + orig_bam = STAR_ALIGN.out.bam // channel: [ val(meta), bam ] + log_final = STAR_ALIGN.out.log_final // channel: [ val(meta), log_final ] + log_out = STAR_ALIGN.out.log_out // channel: [ val(meta), log_out ] + log_progress = STAR_ALIGN.out.log_progress // channel: [ val(meta), log_progress ] + bam_sorted = STAR_ALIGN.out.bam_sorted // channel: [ val(meta), bam_sorted ] + bam_transcript = STAR_ALIGN.out.bam_transcript // channel: [ val(meta), bam_transcript ] + fastq = STAR_ALIGN.out.fastq // channel: [ val(meta), fastq ] + tab = STAR_ALIGN.out.tab // channel: [ val(meta), tab ] + + bam = BAM_SORT_STATS_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] + bai = BAM_SORT_STATS_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] + stats = BAM_SORT_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] + flagstat = BAM_SORT_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_SORT_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] + + versions = ch_versions // channel: [ versions.yml ] +} From ccbc71ccd399ab477a16b6171356091d18debbf7 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 16:10:16 +0200 Subject: [PATCH 037/201] Refactor and rename bam_filter_bamtools --- subworkflows/local/bam_filter_bamtools.nf | 94 +++++++++++++++++++++++ 1 file changed, 94 insertions(+) create mode 100644 subworkflows/local/bam_filter_bamtools.nf diff --git a/subworkflows/local/bam_filter_bamtools.nf b/subworkflows/local/bam_filter_bamtools.nf new file mode 100644 index 00000000..a09876b2 --- /dev/null +++ b/subworkflows/local/bam_filter_bamtools.nf @@ -0,0 +1,94 @@ +include { SAMTOOLS_SORT } from '../../modules/nf-core/samtools/sort/main' +include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' +include { BAM_SORT_STATS_SAMTOOLS } from '../nf-core/bam_sort_stats_samtools/main' +include { BAM_STATS_SAMTOOLS } from '../nf-core/bam_stats_samtools/main' + +include { BAMTOOLS_FILTER } from '../../modules/local/bamtools_filter' +include { BAM_REMOVE_ORPHANS } from '../../modules/local/bam_remove_orphans' + +workflow BAM_FILTER_BAMTOOLS { + take: + ch_bam_bai // channel: [ val(meta), [ bam ], [bai] ] + ch_bed // channel: [ bed ] + ch_fasta // channel: [ fasta ] + ch_bamtools_filter_se_config // channel: [ config_file ] + ch_bamtools_filter_pe_config // channel: [ config_file ] + + main: + + ch_versions = Channel.empty() + + // + // Filter BAM file with BAMTools + // + BAMTOOLS_FILTER ( + ch_bam_bai, + ch_bed, + ch_bamtools_filter_se_config, + ch_bamtools_filter_pe_config + ) + ch_versions = ch_versions.mix(BAMTOOLS_FILTER.out.versions.first()) + + BAMTOOLS_FILTER + .out + .bam + .branch { + meta, bam -> + single_end: meta.single_end + return [ meta, bam ] + paired_end: !meta.single_end + return [ meta, bam ] + } + .set { ch_bam } + + // + // Index SE BAM file + // + SAMTOOLS_INDEX { + ch_bam.single_end + } + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) + + // + // Run samtools stats, flagstat and idxstats on SE BAM + // + BAM_STATS_SAMTOOLS ( + ch_bam.single_end.join(SAMTOOLS_INDEX.out.bai), + ch_fasta + ) + ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions.first()) + + // + // Name sort PE BAM before filtering with pysam + // + SAMTOOLS_SORT ( + ch_bam.paired_end + ) + ch_versions = ch_versions.mix(SAMTOOLS_SORT.out.versions.first()) + + // + // Remove orphan reads from PE BAM file + // + BAM_REMOVE_ORPHANS ( + SAMTOOLS_SORT.out.bam + ) + ch_versions = ch_versions.mix(BAM_REMOVE_ORPHANS.out.versions.first()) + + // + // Sort, index PE BAM file and run samtools stats, flagstat and idxstats + // + BAM_SORT_STATS_SAMTOOLS ( + BAM_REMOVE_ORPHANS.out.bam, + ch_fasta + ) + ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions.first()) + + emit: + name_bam = SAMTOOLS_SORT.out.bam // channel: [ val(meta), [ bam ] ] + bam = BAM_SORT_STATS_SAMTOOLS.out.bam.mix(ch_bam.single_end) // channel: [ val(meta), [ bam ] ] + bai = BAM_SORT_STATS_SAMTOOLS.out.bai.mix(SAMTOOLS_INDEX.out.bai) // channel: [ val(meta), [ bai ] ] + stats = BAM_SORT_STATS_SAMTOOLS.out.stats.mix(BAM_STATS_SAMTOOLS.out.stats) // channel: [ val(meta), [ stats ] ] + flagstat = BAM_SORT_STATS_SAMTOOLS.out.flagstat.mix(BAM_STATS_SAMTOOLS.out.flagstat) // channel: [ val(meta), [ flagstat ] ] + idxstats = BAM_SORT_STATS_SAMTOOLS.out.idxstats.mix(BAM_STATS_SAMTOOLS.out.idxstats) // channel: [ val(meta), [ idxstats ] ] + versions = ch_versions // channel: [ versions.yml ] +} From 0230090cc51443955e07ac2e21602cdb3bfe2e16 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 16:11:27 +0200 Subject: [PATCH 038/201] Refactor code to use nf-core official subworkflows --- conf/modules.config | 14 +++--- subworkflows/local/prepare_genome.nf | 6 ++- workflows/chipseq.nf | 75 ++++++++++++++++++---------- 3 files changed, 61 insertions(+), 34 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 01316ea6..809e6ec1 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -181,7 +181,7 @@ if (!params.skip_trimming) { } process { - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:ALIGN_.*:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' { + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:.*:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_SORT' { ext.prefix = { "${meta.id}.Lb.sorted" } publishDir = [ path: { "${params.outdir}/${params.aligner}/library" }, @@ -191,7 +191,7 @@ process { ] } - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:ALIGN_.*:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' { + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:.*:BAM_SORT_STATS_SAMTOOLS:SAMTOOLS_INDEX' { publishDir = [ path: { "${params.outdir}/${params.aligner}/library" }, mode: params.publish_dir_mode, @@ -200,7 +200,7 @@ process { ] } - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:ALIGN_.*:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_.*' { + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:.*:BAM_SORT_STATS_SAMTOOLS:BAM_STATS_SAMTOOLS:SAMTOOLS_.*' { ext.prefix = { "${meta.id}.Lb.sorted.bam" } publishDir = [ path: { "${params.outdir}/${params.aligner}/library/samtools_stats/" }, @@ -359,7 +359,7 @@ process { } // Should only be published when paired end data is used and save_align_intermeds is true - withName: 'BAM_FILTER' { + withName: 'BAMTOOLS_FILTER' { ext.prefix = { meta.single_end ? "${meta.id}.mLb.noPublish" : "${meta.id}.mLb.flT.sorted" } publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, @@ -375,7 +375,7 @@ process { publishDir = [ enabled: false ] } - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:FILTER_BAM_BAMTOOLS:BAM_SORT_SAMTOOLS:SAMTOOLS_SORT' { + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:SAMTOOLS_SORT' { ext.prefix = { "${meta.id}.mLb.clN.sorted" } publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, @@ -384,7 +384,7 @@ process { ] } - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:FILTER_BAM_BAMTOOLS:BAM_SORT_SAMTOOLS:SAMTOOLS_INDEX' { + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:SAMTOOLS_INDEX' { ext.prefix = { "${meta.id}.mLb.clN.sorted" } publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, @@ -393,7 +393,7 @@ process { ] } - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:FILTER_BAM_BAMTOOLS:BAM_SORT_SAMTOOLS:BAM_STATS_SAMTOOLS:.*' { + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:BAM_STATS_SAMTOOLS:.*' { ext.prefix = { "${meta.id}.mLb.clN.sorted.bam" } publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary/samtools_stats" }, diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index ed5377bf..456c20d8 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -114,7 +114,8 @@ workflow PREPARE_GENOME { // // Create chromosome sizes file // - ch_chrom_sizes = CUSTOM_GETCHROMSIZES ( ch_fasta ).sizes + ch_chrom_sizes = CUSTOM_GETCHROMSIZES ( [ [:], ch_fasta ] ).sizes.map{ it[1] } + ch_fai = CUSTOM_GETCHROMSIZES.out.fai.map{ it[1] } ch_versions = ch_versions.mix(CUSTOM_GETCHROMSIZES.out.versions) // @@ -123,7 +124,7 @@ workflow PREPARE_GENOME { ch_genome_filtered_bed = Channel.empty() GENOME_BLACKLIST_REGIONS ( - CUSTOM_GETCHROMSIZES.out.sizes, + CUSTOM_GETCHROMSIZES.out.sizes.map{ it[1] }, ch_blacklist.ifEmpty([]) ) ch_genome_filtered_bed = GENOME_BLACKLIST_REGIONS.out.bed @@ -204,6 +205,7 @@ workflow PREPARE_GENOME { emit: fasta = ch_fasta // path: genome.fasta + fai = ch_fai // path: genome.fai gtf = ch_gtf // path: genome.gtf gene_bed = ch_gene_bed // path: gene.bed chrom_sizes = ch_chrom_sizes // path: genome.sizes diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 45de3811..f60c8ab3 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -82,7 +82,8 @@ include { MULTIQC_CUSTOM_PEAKS } from '../modules/local/multiqc_c // include { INPUT_CHECK } from '../subworkflows/local/input_check' include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' -include { FILTER_BAM_BAMTOOLS } from '../subworkflows/local/filter_bam_bamtools' +include { ALIGN_STAR } from '../subworkflows/local/align_star' +include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -118,10 +119,8 @@ include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../module include { FASTQ_FASTQC_UMITOOLS_TRIMGALORE } from '../subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/main' include { FASTQ_ALIGN_BWA } from '../subworkflows/nf-core/fastq_align_bwa/main' include { FASTQ_ALIGN_BOWTIE2 } from '../subworkflows/nf-core/fastq_align_bowtie2/main' - -include { ALIGN_CHROMAP } from '../subworkflows/nf-core/align_chromap' -include { ALIGN_STAR } from '../subworkflows/nf-core/align_star' -include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' +include { FASTQ_ALIGN_CHROMAP } from '../subworkflows/nf-core/fastq_align_chromap/main' +include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -213,20 +212,24 @@ workflow CHIPSEQ { // SUBWORKFLOW: Alignment with Chromap & BAM QC // if (params.aligner == 'chromap') { - ALIGN_CHROMAP ( + FASTQ_ALIGN_CHROMAP ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.chromap_index, PREPARE_GENOME.out.fasta .map { [ [:], it ] - } + }, + [], + [], + [], + [] ) - ch_genome_bam = ALIGN_CHROMAP.out.bam - ch_genome_bam_index = ALIGN_CHROMAP.out.bai - ch_samtools_stats = ALIGN_CHROMAP.out.stats - ch_samtools_flagstat = ALIGN_CHROMAP.out.flagstat - ch_samtools_idxstats = ALIGN_CHROMAP.out.idxstats - ch_versions = ch_versions.mix(ALIGN_CHROMAP.out.versions.first()) + ch_genome_bam = FASTQ_ALIGN_CHROMAP.out.bam + ch_genome_bam_index = FASTQ_ALIGN_CHROMAP.out.bai + ch_samtools_stats = FASTQ_ALIGN_CHROMAP.out.stats + ch_samtools_flagstat = FASTQ_ALIGN_CHROMAP.out.flagstat + ch_samtools_idxstats = FASTQ_ALIGN_CHROMAP.out.idxstats + ch_versions = ch_versions.mix(FASTQ_ALIGN_CHROMAP.out.versions.first()) } // @@ -282,13 +285,14 @@ workflow CHIPSEQ { // // SUBWORKFLOW: Filter BAM file with BamTools // - FILTER_BAM_BAMTOOLS ( + BAM_FILTER_BAMTOOLS ( MARK_DUPLICATES_PICARD.out.bam.join(MARK_DUPLICATES_PICARD.out.bai, by: [0]), PREPARE_GENOME.out.filtered_bed.first(), + PREPARE_GENOME.out.fasta, ch_bamtools_filter_se_config, ch_bamtools_filter_pe_config ) - ch_versions = ch_versions.mix(FILTER_BAM_BAMTOOLS.out.versions.first().ifEmpty(null)) + ch_versions = ch_versions.mix(BAM_FILTER_BAMTOOLS.out.versions.first().ifEmpty(null)) // // MODULE: Preseq coverage analysis @@ -305,12 +309,33 @@ workflow CHIPSEQ { // // MODULE: Picard post alignment QC // + BAM_FILTER_BAMTOOLS + .out + .bam + .map { + [ it[0], it[1], [] ] + } ch_picardcollectmultiplemetrics_multiqc = Channel.empty() if (!params.skip_picard_metrics) { PICARD_COLLECTMULTIPLEMETRICS ( - FILTER_BAM_BAMTOOLS.out.bam, - PREPARE_GENOME.out.fasta, - [] + BAM_FILTER_BAMTOOLS + .out + .bam + .map { + [ it[0], it[1], [] ] + }, + PREPARE_GENOME + .out + .fasta + .map { + [ [:], it ] + }, + PREPARE_GENOME + .out + .fai + .map { + [ [:], it ] + } ) ch_picardcollectmultiplemetrics_multiqc = PICARD_COLLECTMULTIPLEMETRICS.out.metrics ch_versions = ch_versions.mix(PICARD_COLLECTMULTIPLEMETRICS.out.versions.first()) @@ -325,7 +350,7 @@ workflow CHIPSEQ { ch_multiqc_phantompeakqualtools_correlation_multiqc = Channel.empty() if (!params.skip_spp) { PHANTOMPEAKQUALTOOLS ( - FILTER_BAM_BAMTOOLS.out.bam + BAM_FILTER_BAMTOOLS.out.bam ) ch_phantompeakqualtools_spp_multiqc = PHANTOMPEAKQUALTOOLS.out.spp ch_versions = ch_versions.mix(PHANTOMPEAKQUALTOOLS.out.versions.first()) @@ -348,7 +373,7 @@ workflow CHIPSEQ { // MODULE: BedGraph coverage tracks // BEDTOOLS_GENOMECOV ( - FILTER_BAM_BAMTOOLS.out.bam.join(FILTER_BAM_BAMTOOLS.out.flagstat, by: [0]) + BAM_FILTER_BAMTOOLS.out.bam.join(BAM_FILTER_BAMTOOLS.out.flagstat, by: [0]) ) ch_versions = ch_versions.mix(BEDTOOLS_GENOMECOV.out.versions.first()) @@ -393,10 +418,10 @@ workflow CHIPSEQ { // // Create channels: [ meta, [ ip_bam, control_bam ] [ ip_bai, control_bai ] ] // - FILTER_BAM_BAMTOOLS + BAM_FILTER_BAMTOOLS .out .bam - .join(FILTER_BAM_BAMTOOLS.out.bai, by: [0]) + .join(BAM_FILTER_BAMTOOLS.out.bai, by: [0]) .set { ch_genome_bam_bai } ch_genome_bam_bai @@ -697,9 +722,9 @@ workflow CHIPSEQ { MARK_DUPLICATES_PICARD.out.idxstats.collect{it[1]}.ifEmpty([]), MARK_DUPLICATES_PICARD.out.metrics.collect{it[1]}.ifEmpty([]), - FILTER_BAM_BAMTOOLS.out.stats.collect{it[1]}.ifEmpty([]), - FILTER_BAM_BAMTOOLS.out.flagstat.collect{it[1]}.ifEmpty([]), - FILTER_BAM_BAMTOOLS.out.idxstats.collect{it[1]}.ifEmpty([]), + BAM_FILTER_BAMTOOLS.out.stats.collect{it[1]}.ifEmpty([]), + BAM_FILTER_BAMTOOLS.out.flagstat.collect{it[1]}.ifEmpty([]), + BAM_FILTER_BAMTOOLS.out.idxstats.collect{it[1]}.ifEmpty([]), ch_picardcollectmultiplemetrics_multiqc.collect{it[1]}.ifEmpty([]), ch_preseq_multiqc.collect{it[1]}.ifEmpty([]), From 3165a73b7eaacdbb78ea676bb2a1de53a9b65089 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 17:17:02 +0200 Subject: [PATCH 039/201] Simplify code --- workflows/chipseq.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index f60c8ab3..7a01c748 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -292,7 +292,7 @@ workflow CHIPSEQ { ch_bamtools_filter_se_config, ch_bamtools_filter_pe_config ) - ch_versions = ch_versions.mix(BAM_FILTER_BAMTOOLS.out.versions.first().ifEmpty(null)) + ch_versions = ch_versions.mix(BAM_FILTER_BAMTOOLS.out.versions) // // MODULE: Preseq coverage analysis From 30312c3c5962c72757fc7ecfd3e27e84e6ea0c3a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 17:18:45 +0200 Subject: [PATCH 040/201] changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 173b68d1..531befa7 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -11,6 +11,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#317](https://github.com/nf-core/chipseq/issues/317)] Added metro map - [[#288](https://github.com/nf-core/chipseq/issues/291)] Bump `chromap` version 2 and enable all the steps below chromap again when paired-end data is processed. - [[#311](https://github.com/nf-core/chipseq/issues/311)] Add back `--skip_spp` parameter which was unintentionally removed from the code. +- Install available nf-core subworkflows and refactor code accordingly ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 From 603db6303f178d655da36c9bf3986cf710353b76 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 18:57:40 +0200 Subject: [PATCH 041/201] Resolved arguments for bamtools_filteer --- conf/modules.config | 9 ++++++++- 1 file changed, 8 insertions(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 809e6ec1..dfc0c5ec 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -360,7 +360,14 @@ process { // Should only be published when paired end data is used and save_align_intermeds is true withName: 'BAMTOOLS_FILTER' { - ext.prefix = { meta.single_end ? "${meta.id}.mLb.noPublish" : "${meta.id}.mLb.flT.sorted" } + ext.args = { + [ + meta.single_end ? '-F 0x004' : '-F 0x004 -F 0x0008 -f 0x001', + params.keep_dups ? '' : '-F 0x0400', + params.keep_multi_map ? '' : '-q 1' + ].join(' ').trim() + } + ext.prefix = { meta.single_end ? "${meta.id}.mLb.noPublish" : "${meta.id}.mLb.flT.sorted" } //TODO check with atacseq publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, mode: params.publish_dir_mode, From 2637d88d331199b10a5c102ab5c2fc3f70c18eca Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 19:01:08 +0200 Subject: [PATCH 042/201] Update bowtie2/align to fix the container download --- modules.json | 2 +- modules/nf-core/bowtie2/align/main.nf | 16 ++++++++-------- modules/nf-core/bowtie2/align/meta.yml | 5 +++++ 3 files changed, 14 insertions(+), 9 deletions(-) diff --git a/modules.json b/modules.json index 22fd3128..630219e6 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "bowtie2/align": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["fastq_align_bowtie2", "modules"] }, "bowtie2/build": { diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf index c74e376f..3d851866 100644 --- a/modules/nf-core/bowtie2/align/main.nf +++ b/modules/nf-core/bowtie2/align/main.nf @@ -2,14 +2,14 @@ process BOWTIE2_ALIGN { tag "$meta.id" label "process_high" - conda (params.enable_conda ? "bioconda::bowtie2=2.4.4 bioconda::samtools=1.15.1 conda-forge::pigz=2.6" : null) - container "${ workflow.containerEngine == "singularity" && !task.ext.singularity_pull_docker_container ? - "https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0" : - "quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:1744f68fe955578c63054b55309e05b41c37a80d-0" }" + conda "bioconda::bowtie2=2.4.4 bioconda::samtools=1.16.1 conda-forge::pigz=2.6" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' : + 'quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" input: - tuple val(meta), path(reads) - path index + tuple val(meta) , path(reads) + tuple val(meta2), path(index) val save_unaligned val sort_bam @@ -40,8 +40,8 @@ process BOWTIE2_ALIGN { def samtools_command = sort_bam ? 'sort' : 'view' """ - INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/.rev.1.bt2//"` - [ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed "s/.rev.1.bt2l//"` + INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/\\.rev.1.bt2\$//"` + [ -z "\$INDEX" ] && INDEX=`find -L ./ -name "*.rev.1.bt2l" | sed "s/\\.rev.1.bt2l\$//"` [ -z "\$INDEX" ] && echo "Bowtie2 index files not found" 1>&2 && exit 1 bowtie2 \\ diff --git a/modules/nf-core/bowtie2/align/meta.yml b/modules/nf-core/bowtie2/align/meta.yml index 42ba0f96..c8e9a001 100644 --- a/modules/nf-core/bowtie2/align/meta.yml +++ b/modules/nf-core/bowtie2/align/meta.yml @@ -27,6 +27,11 @@ input: description: | List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test', single_end:false ] - index: type: file description: Bowtie2 genome index files From 6a75945dc4bacacb93f11941a09eeec8539f2d39 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 19:08:38 +0200 Subject: [PATCH 043/201] Remove code used for view --- workflows/chipseq.nf | 6 ------ 1 file changed, 6 deletions(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 7a01c748..e448bda1 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -309,12 +309,6 @@ workflow CHIPSEQ { // // MODULE: Picard post alignment QC // - BAM_FILTER_BAMTOOLS - .out - .bam - .map { - [ it[0], it[1], [] ] - } ch_picardcollectmultiplemetrics_multiqc = Channel.empty() if (!params.skip_picard_metrics) { PICARD_COLLECTMULTIPLEMETRICS ( From b00897d31317c96cfb797c23c8e0e452f8f47e02 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 22:04:04 +0200 Subject: [PATCH 044/201] Feed missing arguments to star subworkflow --- workflows/chipseq.nf | 4 +++- 1 file changed, 3 insertions(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index e448bda1..71d7dfe8 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -238,7 +238,9 @@ workflow CHIPSEQ { if (params.aligner == 'star') { ALIGN_STAR ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, - PREPARE_GENOME.out.star_index + PREPARE_GENOME.out.star_index, + PREPARE_GENOME.out.fasta, + params.seq_center ?: '' ) ch_genome_bam = ALIGN_STAR.out.bam ch_genome_bam_index = ALIGN_STAR.out.bai From be423bf39a58d36e1a68cadea59f62ae9ec362a4 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 22:12:05 +0200 Subject: [PATCH 045/201] Update chromap/chromap --- modules.json | 2 +- modules/nf-core/chromap/chromap/main.nf | 2 +- modules/nf-core/chromap/chromap/meta.yml | 7 ++++++- 3 files changed, 8 insertions(+), 3 deletions(-) diff --git a/modules.json b/modules.json index 630219e6..55237ed3 100644 --- a/modules.json +++ b/modules.json @@ -27,7 +27,7 @@ }, "chromap/chromap": { "branch": "master", - "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", + "git_sha": "b7f0dbc07af00c0ccdc7c2df06efee8786e976ac", "installed_by": ["fastq_align_chromap", "modules"] }, "chromap/index": { diff --git a/modules/nf-core/chromap/chromap/main.nf b/modules/nf-core/chromap/chromap/main.nf index 5b7631cb..69a4bc1b 100644 --- a/modules/nf-core/chromap/chromap/main.nf +++ b/modules/nf-core/chromap/chromap/main.nf @@ -10,7 +10,7 @@ process CHROMAP_CHROMAP { input: tuple val(meta), path(reads) tuple val(meta2), path(fasta) - tuple val(meta2), path(index) + tuple val(meta3), path(index) path barcodes path whitelist path chr_order diff --git a/modules/nf-core/chromap/chromap/meta.yml b/modules/nf-core/chromap/chromap/meta.yml index 10ce8f58..05f70cf0 100644 --- a/modules/nf-core/chromap/chromap/meta.yml +++ b/modules/nf-core/chromap/chromap/meta.yml @@ -36,12 +36,17 @@ input: - meta2: type: map description: | - Groovy Map containing sample information + Groovy Map containing information for the fasta e.g. [ id:'test' ] - fasta: type: file description: | The fasta reference file. + - meta3: + type: map + description: | + Groovy Map containing information for the index + e.g. [ id:'test' ] - index: type: file description: | From 7cc3041ad21667f326ae9e7703b9dd810f305186 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 22:18:58 +0200 Subject: [PATCH 046/201] Update star_align --- modules/local/star_align.nf | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/modules/local/star_align.nf b/modules/local/star_align.nf index f960045f..f1988bea 100644 --- a/modules/local/star_align.nf +++ b/modules/local/star_align.nf @@ -11,6 +11,7 @@ process STAR_ALIGN { input: tuple val(meta), path(reads) path index + val seq_center output: tuple val(meta), path('*d.out.bam') , emit: bam @@ -25,10 +26,13 @@ process STAR_ALIGN { tuple val(meta), path('*fastq.gz') , optional:true, emit: fastq tuple val(meta), path('*.tab') , optional:true, emit: tab + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def seq_center = params.seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$params.seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'" + def seq_center_tag = seq_center ? "--outSAMattrRGline ID:$prefix 'CN:$seq_center' 'SM:$prefix'" : "--outSAMattrRGline ID:$prefix 'SM:$prefix'" def out_sam_type = (args.contains('--outSAMtype')) ? '' : '--outSAMtype BAM Unsorted' def mv_unsorted_bam = (args.contains('--outSAMtype BAM Unsorted SortedByCoordinate')) ? "mv ${prefix}.Aligned.out.bam ${prefix}.Aligned.unsort.out.bam" : '' """ @@ -38,7 +42,7 @@ process STAR_ALIGN { --runThreadN $task.cpus \\ --outFileNamePrefix $prefix. \\ $out_sam_type \\ - $seq_center \\ + $seq_center_tag \\ $args $mv_unsorted_bam if [ -f ${prefix}.Unmapped.out.mate1 ]; then From 403a8e59cfe66b785833fd4d4b6e40a40e3ab112 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 22:21:32 +0200 Subject: [PATCH 047/201] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 531befa7..b6d20d60 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -12,6 +12,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#288](https://github.com/nf-core/chipseq/issues/291)] Bump `chromap` version 2 and enable all the steps below chromap again when paired-end data is processed. - [[#311](https://github.com/nf-core/chipseq/issues/311)] Add back `--skip_spp` parameter which was unintentionally removed from the code. - Install available nf-core subworkflows and refactor code accordingly +- [[#318](https://github.com/nf-core/chipseq/issues/318)] Update `bowtie2/align` module to fix issue when downloading its singularity image. ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 From aece0fb6c96c259e83b357100c66348e9f2aad1a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 18 Apr 2023 22:48:01 +0200 Subject: [PATCH 048/201] Update bowtie2/build --- modules.json | 2 +- modules/nf-core/bowtie2/build/main.nf | 8 ++++---- modules/nf-core/bowtie2/build/meta.yml | 10 ++++++++++ subworkflows/local/prepare_genome.nf | 6 +++--- 4 files changed, 18 insertions(+), 8 deletions(-) diff --git a/modules.json b/modules.json index 55237ed3..28fb02ca 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "bowtie2/build": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] }, "bwa/index": { diff --git a/modules/nf-core/bowtie2/build/main.nf b/modules/nf-core/bowtie2/build/main.nf index a4da62d0..551893af 100644 --- a/modules/nf-core/bowtie2/build/main.nf +++ b/modules/nf-core/bowtie2/build/main.nf @@ -2,17 +2,17 @@ process BOWTIE2_BUILD { tag "$fasta" label 'process_high' - conda (params.enable_conda ? 'bioconda::bowtie2=2.4.4' : null) + conda "bioconda::bowtie2=2.4.4" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bowtie2:2.4.4--py39hbb4e92a_0' : 'quay.io/biocontainers/bowtie2:2.4.4--py39hbb4e92a_0' }" input: - path fasta + tuple val(meta), path(fasta) output: - path 'bowtie2' , emit: index - path "versions.yml" , emit: versions + tuple val(meta), path('bowtie2') , emit: index + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/bowtie2/build/meta.yml b/modules/nf-core/bowtie2/build/meta.yml index 2da9a217..0240224d 100644 --- a/modules/nf-core/bowtie2/build/meta.yml +++ b/modules/nf-core/bowtie2/build/meta.yml @@ -16,10 +16,20 @@ tools: doi: 10.1038/nmeth.1923 licence: ["GPL-3.0-or-later"] input: + - meta: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test', single_end:false ] - fasta: type: file description: Input genome fasta file output: + - meta: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test', single_end:false ] - index: type: file description: Bowtie2 genome index files diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 456c20d8..4cb2d164 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -156,13 +156,13 @@ workflow PREPARE_GENOME { if (prepare_tool_index == 'bowtie2') { if (params.bowtie2_index) { if (params.bowtie2_index.endsWith('.tar.gz')) { - ch_bowtie2_index = UNTAR_BOWTIE2_INDEX ( [ [:], params.bowtie2_index ] ).untar.map{ it[1] } + ch_bowtie2_index = UNTAR_BOWTIE2_INDEX ( [ [:], params.bowtie2_index ] ).untar ch_versions = ch_versions.mix(UNTAR_BOWTIE2_INDEX.out.versions) } else { - ch_bowtie2_index = file(params.bowtie2_index) + ch_bowtie2_index = [ [:], file(params.bowtie2_index) ] } } else { - ch_bowtie2_index = BOWTIE2_BUILD ( ch_fasta ).index + ch_bowtie2_index = BOWTIE2_BUILD ( [ [:], ch_fasta ] ).index ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions) } } From 0f4af2c50c533413178a9f77668fd288d44fa44e Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 19 Apr 2023 12:07:02 +0200 Subject: [PATCH 049/201] Fix samplesheet control column in docs examples --- CHANGELOG.md | 1 + docs/usage.md | 38 +++++++++++++++++++------------------- 2 files changed, 20 insertions(+), 19 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index b6d20d60..88152e18 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -13,6 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#311](https://github.com/nf-core/chipseq/issues/311)] Add back `--skip_spp` parameter which was unintentionally removed from the code. - Install available nf-core subworkflows and refactor code accordingly - [[#318](https://github.com/nf-core/chipseq/issues/318)] Update `bowtie2/align` module to fix issue when downloading its singularity image. +- [[#320](https://github.com/nf-core/chipseq/issues/320)] Fix samplesheet control column in documentation examples. ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 diff --git a/docs/usage.md b/docs/usage.md index 0bdce0c3..74143398 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -18,11 +18,11 @@ The `sample` identifiers have to be the same when you have re-sequenced the same ```console sample,fastq_1,fastq_2,antibody,control -WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L003_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT +WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP1 +WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 +WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 +WT_BCATENIN_IP_REP2,BLA203A25_S16_L003_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 +WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP3 WT_INPUT_REP1,BLA203A6_S32_L006_R1_001.fastq.gz,,, WT_INPUT_REP2,BLA203A30_S21_L001_R1_001.fastq.gz,,, WT_INPUT_REP2,BLA203A30_S21_L002_R1_001.fastq.gz,,, @@ -41,20 +41,20 @@ A final design file may look something like the one below. This is for two antib ```console sample,fastq_1,fastq_2,antibody,control -WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT -NAIVE_BCATENIN_IP_REP1,BLA203A7_S60_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L002_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP3,BLA203A64_S55_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -WT_TCF4_IP_REP1,BLA203A3_S29_L006_R1_001.fastq.gz,,TCF4,WT_INPUT -WT_TCF4_IP_REP2,BLA203A27_S18_L001_R1_001.fastq.gz,,TCF4,WT_INPUT -WT_TCF4_IP_REP3,BLA203A51_S42_L001_R1_001.fastq.gz,,TCF4,WT_INPUT -NAIVE_TCF4_IP_REP1,BLA203A9_S62_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT -NAIVE_TCF4_IP_REP2,BLA203A45_S36_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT -NAIVE_TCF4_IP_REP3,BLA203A66_S57_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT +WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP1 +WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 +WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 +WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP3 +NAIVE_BCATENIN_IP_REP1,BLA203A7_S60_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP1 +NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP2 +NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L002_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP2 +NAIVE_BCATENIN_IP_REP3,BLA203A64_S55_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP3 +WT_TCF4_IP_REP1,BLA203A3_S29_L006_R1_001.fastq.gz,,TCF4,WT_INPUT_REP1 +WT_TCF4_IP_REP2,BLA203A27_S18_L001_R1_001.fastq.gz,,TCF4,WT_INPUT_REP2 +WT_TCF4_IP_REP3,BLA203A51_S42_L001_R1_001.fastq.gz,,TCF4,WT_INPUT_REP3 +NAIVE_TCF4_IP_REP1,BLA203A9_S62_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT_REP1 +NAIVE_TCF4_IP_REP2,BLA203A45_S36_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT_REP2 +NAIVE_TCF4_IP_REP3,BLA203A66_S57_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT_REP3 WT_INPUT_REP1,BLA203A6_S32_L006_R1_001.fastq.gz,,, WT_INPUT_REP2,BLA203A30_S21_L001_R1_001.fastq.gz,,, WT_INPUT_REP3,BLA203A31_S21_L003_R1_001.fastq.gz,,, From 683428a6760b192cd46edd35879fb0cbb6260125 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 19 Apr 2023 12:07:57 +0200 Subject: [PATCH 050/201] Try to clarify read_length docs --- CHANGELOG.md | 1 + nextflow_schema.json | 3 ++- 2 files changed, 3 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 88152e18..b0266c0d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -14,6 +14,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Install available nf-core subworkflows and refactor code accordingly - [[#318](https://github.com/nf-core/chipseq/issues/318)] Update `bowtie2/align` module to fix issue when downloading its singularity image. - [[#320](https://github.com/nf-core/chipseq/issues/320)] Fix samplesheet control column in documentation examples. +- [[#328](https://github.com/nf-core/chipseq/issues/328)] Modify documentation to clarify that is necessary to provide the `--read_length` when `--genome` is set and `--macs_gsize` has not provided. ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 diff --git a/nextflow_schema.json b/nextflow_schema.json index 77a094e3..7cadb7c4 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -37,6 +37,7 @@ "type": "integer", "description": "Read length used to calculate MACS2 genome size for peak calling if `--macs_gsize` isn't provided.", "fa_icon": "fas fa-chart-area", + "help_text": "Read length is used to calculate MACS2 genome size using the following [approach](https://deeptools.readthedocs.io/en/develop/content/feature/effectiveGenomeSize.html#effective-genome-size). For all the genomes present in the `igenomes.config` the genome size has been precomputed and the read length is then used to retrieve the corresponding value", "enum": [50, 75, 100, 150, 200] }, "outdir": { @@ -132,7 +133,7 @@ "macs_gsize": { "type": "number", "description": "Effective genome size parameter required by MACS2.", - "help_text": "[Effective genome size](https://github.com/taoliu/MACS#-g--gsize) parameter required by MACS2. If using an iGenomes reference these have been provided when `--genome` is set as *GRCh37*, *GRCh38*, *GRCm38*, *WBcel235*, *BDGP6*, *R64-1-1*, *EF2*, *hg38*, *hg19* and *mm10*. For other genomes, if this parameter is not specified then the MACS2 peak-calling and differential analysis will be skipped.", + "help_text": "[Effective genome size](https://github.com/taoliu/MACS#-g--gsize) parameter required by MACS2. If using an iGenomes reference these have been provided for any of the genomes available in the igenomes.config, and for the following read lengths (50,75,100,150,200) that should be set using the `--read_length` parameter. For other genomes, if this parameter is not specified then the MACS2 peak-calling and differential analysis will be skipped.", "fa_icon": "fas fa-arrows-alt-h" }, "blacklist": { From 58bd1f6bc3dd4f1440abfaacc419d4d30a0e8dbd Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 19 Apr 2023 22:20:33 +0200 Subject: [PATCH 051/201] Fix macs_gsize help text --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 7cadb7c4..3e876f04 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -133,7 +133,7 @@ "macs_gsize": { "type": "number", "description": "Effective genome size parameter required by MACS2.", - "help_text": "[Effective genome size](https://github.com/taoliu/MACS#-g--gsize) parameter required by MACS2. If using an iGenomes reference these have been provided for any of the genomes available in the igenomes.config, and for the following read lengths (50,75,100,150,200) that should be set using the `--read_length` parameter. For other genomes, if this parameter is not specified then the MACS2 peak-calling and differential analysis will be skipped.", + "help_text": "[Effective genome size](https://github.com/taoliu/MACS#-g--gsize) parameter required by MACS2. If using an iGenomes reference these have been provided for any of the genomes available in the igenomes.config, and for the following read lengths (50,75,100,150,200) that should be set using the `--read_length` parameter. For other genomes, if this parameter is not specified it will be inferred using the provided `--read_length` or otherwise the pipeline execution will stop with an error.", "fa_icon": "fas fa-arrows-alt-h" }, "blacklist": { From f4cbf2a53ebff87395ec41d9d01b63e5d15b7b64 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 19 Apr 2023 22:25:17 +0200 Subject: [PATCH 052/201] Refine read_length help text --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 3e876f04..ee2b1ba8 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -37,7 +37,7 @@ "type": "integer", "description": "Read length used to calculate MACS2 genome size for peak calling if `--macs_gsize` isn't provided.", "fa_icon": "fas fa-chart-area", - "help_text": "Read length is used to calculate MACS2 genome size using the following [approach](https://deeptools.readthedocs.io/en/develop/content/feature/effectiveGenomeSize.html#effective-genome-size). For all the genomes present in the `igenomes.config` the genome size has been precomputed and the read length is then used to retrieve the corresponding value", + "help_text": "Read length together with the genome fasta are used to calculate MACS2 genome size using the `khmer` program as explained [here](https://deeptools.readthedocs.io/en/develop/content/feature/effectiveGenomeSize.html#effective-genome-size). For all the genomes present in the `igenomes.config` the genome size has been already precomputed and the read length is then used to retrieve the corresponding value", "enum": [50, 75, 100, 150, 200] }, "outdir": { From 23efb256ff8145c1fc22f2fe819bbbd15df98d0c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 19 Apr 2023 22:27:44 +0200 Subject: [PATCH 053/201] Update readme since the steps below mapping can be performed when chromap is used with PE reads --- README.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/README.md b/README.md index 232ef8b8..b6c474ab 100644 --- a/README.md +++ b/README.md @@ -32,7 +32,7 @@ You can find numerous talks on the [nf-core events page](https://nf-co.re/events 2. Adapter trimming ([`Trim Galore!`](https://www.bioinformatics.babraham.ac.uk/projects/trim_galore/)) 3. Choice of multiple aligners 1.([`BWA`](https://sourceforge.net/projects/bio-bwa/files/)) - 2.([`Chromap`](https://github.com/haowenz/chromap)). **For paired-end reads only working until mapping steps, see [here](https://github.com/nf-core/chipseq/issues/291)** + 2.([`Chromap`](https://github.com/haowenz/chromap)) 3.([`Bowtie2`](http://bowtie-bio.sourceforge.net/bowtie2/index.shtml)) 4.([`STAR`](https://github.com/alexdobin/STAR)) 4. Mark duplicates ([`picard`](https://broadinstitute.github.io/picard/)) From eeb019891b3aab473285fec2dd500444e94396e2 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 21 Apr 2023 12:47:53 +0200 Subject: [PATCH 054/201] Update modules --- modules.json | 56 +++++++++---------- modules/nf-core/bwa/index/main.nf | 24 ++++++-- modules/nf-core/bwa/index/meta.yml | 10 ++++ modules/nf-core/bwa/mem/main.nf | 10 ++-- modules/nf-core/bwa/mem/meta.yml | 5 ++ .../custom/dumpsoftwareversions/main.nf | 6 +- .../templates/dumpsoftwareversions.py | 0 .../nf-core/deeptools/computematrix/main.nf | 2 +- .../nf-core/deeptools/computematrix/meta.yml | 1 - .../nf-core/deeptools/plotfingerprint/main.nf | 2 +- .../deeptools/plotfingerprint/meta.yml | 1 - modules/nf-core/deeptools/plotheatmap/main.nf | 2 +- .../nf-core/deeptools/plotheatmap/meta.yml | 1 - modules/nf-core/deeptools/plotprofile/main.nf | 2 +- .../nf-core/deeptools/plotprofile/meta.yml | 1 - modules/nf-core/gffread/main.nf | 2 +- modules/nf-core/gunzip/main.nf | 2 +- modules/nf-core/gunzip/meta.yml | 34 +++++------ modules/nf-core/homer/annotatepeaks/main.nf | 2 +- modules/nf-core/khmer/uniquekmers/main.nf | 2 +- modules/nf-core/khmer/uniquekmers/meta.yml | 2 +- modules/nf-core/macs2/callpeak/main.nf | 2 +- modules/nf-core/macs2/callpeak/meta.yml | 4 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/phantompeakqualtools/main.nf | 2 +- modules/nf-core/phantompeakqualtools/meta.yml | 10 +++- modules/nf-core/picard/markduplicates/main.nf | 15 +++-- .../nf-core/picard/markduplicates/meta.yml | 10 +++- modules/nf-core/picard/mergesamfiles/main.nf | 12 ++-- modules/nf-core/preseq/lcextrap/main.nf | 2 +- modules/nf-core/preseq/lcextrap/meta.yml | 2 +- modules/nf-core/samtools/flagstat/main.nf | 6 +- modules/nf-core/samtools/flagstat/meta.yml | 2 +- modules/nf-core/samtools/idxstats/main.nf | 8 +-- modules/nf-core/samtools/idxstats/meta.yml | 2 +- modules/nf-core/samtools/index/main.nf | 6 +- modules/nf-core/samtools/index/meta.yml | 2 +- modules/nf-core/samtools/sort/main.nf | 6 +- modules/nf-core/samtools/sort/meta.yml | 2 +- modules/nf-core/samtools/stats/main.nf | 6 +- modules/nf-core/samtools/stats/meta.yml | 14 ++--- modules/nf-core/subread/featurecounts/main.nf | 2 +- modules/nf-core/trimgalore/main.nf | 31 ++++------ modules/nf-core/trimgalore/meta.yml | 2 +- modules/nf-core/ucsc/bedgraphtobigwig/main.nf | 2 +- .../nf-core/ucsc/bedgraphtobigwig/meta.yml | 6 +- 46 files changed, 179 insertions(+), 150 deletions(-) mode change 100755 => 100644 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules.json b/modules.json index 28fb02ca..d6769afc 100644 --- a/modules.json +++ b/modules.json @@ -17,12 +17,12 @@ }, "bwa/index": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["fastq_align_bwa", "modules"] }, "chromap/chromap": { @@ -37,7 +37,7 @@ }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "7101db4432d3268b7fcb5b8f75fa0a022dc5561b", "installed_by": ["modules"] }, "custom/getchromsizes": { @@ -47,22 +47,22 @@ }, "deeptools/computematrix": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "deeptools/plotfingerprint": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "deeptools/plotheatmap": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "deeptools/plotprofile": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "fastqc": { @@ -72,37 +72,37 @@ }, "gffread": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] }, "gunzip": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "homer/annotatepeaks": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] }, "khmer/uniquekmers": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "2f398a6342db61e7ab0f4cb4d395eed74b65db7c", "installed_by": ["modules"] }, "macs2/callpeak": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "ee80d14721e76e2e079103b8dcd5d57129e584ba", "installed_by": ["modules"] }, "phantompeakqualtools": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "2f398a6342db61e7ab0f4cb4d395eed74b65db7c", "installed_by": ["modules"] }, "picard/collectmultiplemetrics": { @@ -112,57 +112,57 @@ }, "picard/markduplicates": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "75027bf77472b1f4fd2cdd7e46f83119dfb0f2c6", "installed_by": ["modules"] }, "picard/mergesamfiles": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "75027bf77472b1f4fd2cdd7e46f83119dfb0f2c6", "installed_by": ["modules"] }, "preseq/lcextrap": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["bam_stats_samtools", "modules"] + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", + "installed_by": ["modules", "bam_stats_samtools"] }, "samtools/idxstats": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["bam_stats_samtools", "modules"] + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", + "installed_by": ["modules", "bam_stats_samtools"] }, "samtools/index": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["bam_sort_stats_samtools", "modules"] }, "samtools/sort": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["bam_sort_stats_samtools", "modules"] }, "samtools/stats": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", - "installed_by": ["bam_stats_samtools", "modules"] + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", + "installed_by": ["modules", "bam_stats_samtools"] }, "subread/featurecounts": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", "installed_by": ["modules"] }, "trimgalore": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "64a3dbc0a30a94cdaed7869d8e34fbb85e886614", "installed_by": ["modules", "fastq_fastqc_umitools_trimgalore"] }, "ucsc/bedgraphtobigwig": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", "installed_by": ["modules"] }, "umitools/extract": { diff --git a/modules/nf-core/bwa/index/main.nf b/modules/nf-core/bwa/index/main.nf index aa75ae5d..7ccf3110 100644 --- a/modules/nf-core/bwa/index/main.nf +++ b/modules/nf-core/bwa/index/main.nf @@ -2,17 +2,17 @@ process BWA_INDEX { tag "$fasta" label 'process_single' - conda (params.enable_conda ? "bioconda::bwa=0.7.17" : null) + conda "bioconda::bwa=0.7.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bwa:0.7.17--hed695b0_7' : 'quay.io/biocontainers/bwa:0.7.17--hed695b0_7' }" input: - path fasta + tuple val(meta), path(fasta) output: - path "bwa" , emit: index - path "versions.yml", emit: versions + tuple val(meta), path(bwa) , emit: index + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -32,4 +32,20 @@ process BWA_INDEX { bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') END_VERSIONS """ + + stub: + """ + mkdir bwa + + touch bwa/genome.amb + touch bwa/genome.ann + touch bwa/genome.bwt + touch bwa/genome.pac + touch bwa/genome.sa + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/bwa/index/meta.yml b/modules/nf-core/bwa/index/meta.yml index 2bbd81d9..2c6cfcd7 100644 --- a/modules/nf-core/bwa/index/meta.yml +++ b/modules/nf-core/bwa/index/meta.yml @@ -15,10 +15,20 @@ tools: arxiv: arXiv:1303.3997 licence: ["GPL-3.0-or-later"] input: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] - fasta: type: file description: Input genome fasta file output: + - meta: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] - index: type: file description: BWA genome index files diff --git a/modules/nf-core/bwa/mem/main.nf b/modules/nf-core/bwa/mem/main.nf index f55af944..cbf34c25 100644 --- a/modules/nf-core/bwa/mem/main.nf +++ b/modules/nf-core/bwa/mem/main.nf @@ -2,14 +2,14 @@ process BWA_MEM { tag "$meta.id" label 'process_high' - conda (params.enable_conda ? "bioconda::bwa=0.7.17 bioconda::samtools=1.15.1" : null) + conda "bioconda::bwa=0.7.17 bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:8110a70be2bfe7f75a2ea7f2a89cda4cc7732095-0' : - 'quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:8110a70be2bfe7f75a2ea7f2a89cda4cc7732095-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:219b6c272b25e7e642ae3ff0bf0c5c81a5135ab4-0' : + 'quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:219b6c272b25e7e642ae3ff0bf0c5c81a5135ab4-0' }" input: tuple val(meta), path(reads) - path index + tuple val(meta2), path(index) val sort_bam output: @@ -25,7 +25,7 @@ process BWA_MEM { def prefix = task.ext.prefix ?: "${meta.id}" def samtools_command = sort_bam ? 'sort' : 'view' """ - INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'` + INDEX=`find -L ./ -name "*.amb" | sed 's/\\.amb\$//'` bwa mem \\ $args \\ diff --git a/modules/nf-core/bwa/mem/meta.yml b/modules/nf-core/bwa/mem/meta.yml index f84c5227..62357bf8 100644 --- a/modules/nf-core/bwa/mem/meta.yml +++ b/modules/nf-core/bwa/mem/meta.yml @@ -28,6 +28,11 @@ input: description: | List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information. + e.g. [ id:'test', single_end:false ] - index: type: file description: BWA genome index files diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 3df21765..800a6099 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.13" + conda "bioconda::multiqc=1.14" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py old mode 100755 new mode 100644 diff --git a/modules/nf-core/deeptools/computematrix/main.nf b/modules/nf-core/deeptools/computematrix/main.nf index 96dfef3c..024b8797 100644 --- a/modules/nf-core/deeptools/computematrix/main.nf +++ b/modules/nf-core/deeptools/computematrix/main.nf @@ -2,7 +2,7 @@ process DEEPTOOLS_COMPUTEMATRIX { tag "$meta.id" label 'process_high' - conda (params.enable_conda ? 'bioconda::deeptools=3.5.1' : null) + conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" diff --git a/modules/nf-core/deeptools/computematrix/meta.yml b/modules/nf-core/deeptools/computematrix/meta.yml index eaa990dd..8a148f5d 100644 --- a/modules/nf-core/deeptools/computematrix/meta.yml +++ b/modules/nf-core/deeptools/computematrix/meta.yml @@ -8,7 +8,6 @@ keywords: tools: - deeptools: description: A set of user-friendly tools for normalization and visualization of deep-sequencing data - homepage: documentation: https://deeptools.readthedocs.io/en/develop/index.html tool_dev_url: https://github.com/deeptools/deepTools doi: "10.1093/nar/gku365" diff --git a/modules/nf-core/deeptools/plotfingerprint/main.nf b/modules/nf-core/deeptools/plotfingerprint/main.nf index 83613be7..233b3bae 100644 --- a/modules/nf-core/deeptools/plotfingerprint/main.nf +++ b/modules/nf-core/deeptools/plotfingerprint/main.nf @@ -2,7 +2,7 @@ process DEEPTOOLS_PLOTFINGERPRINT { tag "$meta.id" label 'process_high' - conda (params.enable_conda ? 'bioconda::deeptools=3.5.1' : null) + conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" diff --git a/modules/nf-core/deeptools/plotfingerprint/meta.yml b/modules/nf-core/deeptools/plotfingerprint/meta.yml index 07c25748..29ddf893 100644 --- a/modules/nf-core/deeptools/plotfingerprint/meta.yml +++ b/modules/nf-core/deeptools/plotfingerprint/meta.yml @@ -8,7 +8,6 @@ keywords: tools: - deeptools: description: A set of user-friendly tools for normalization and visualization of deep-sequencing data - homepage: documentation: https://deeptools.readthedocs.io/en/develop/index.html tool_dev_url: https://github.com/deeptools/deepTools doi: "10.1093/nar/gku365" diff --git a/modules/nf-core/deeptools/plotheatmap/main.nf b/modules/nf-core/deeptools/plotheatmap/main.nf index 1e402e39..3b171b73 100644 --- a/modules/nf-core/deeptools/plotheatmap/main.nf +++ b/modules/nf-core/deeptools/plotheatmap/main.nf @@ -2,7 +2,7 @@ process DEEPTOOLS_PLOTHEATMAP { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? 'bioconda::deeptools=3.5.1' : null) + conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" diff --git a/modules/nf-core/deeptools/plotheatmap/meta.yml b/modules/nf-core/deeptools/plotheatmap/meta.yml index ea206fb6..9296f503 100644 --- a/modules/nf-core/deeptools/plotheatmap/meta.yml +++ b/modules/nf-core/deeptools/plotheatmap/meta.yml @@ -8,7 +8,6 @@ keywords: tools: - deeptools: description: A set of user-friendly tools for normalization and visualization of deep-sequencing data - homepage: documentation: https://deeptools.readthedocs.io/en/develop/index.html tool_dev_url: https://github.com/deeptools/deepTools doi: "10.1093/nar/gku365" diff --git a/modules/nf-core/deeptools/plotprofile/main.nf b/modules/nf-core/deeptools/plotprofile/main.nf index d83a9493..b5fa57df 100644 --- a/modules/nf-core/deeptools/plotprofile/main.nf +++ b/modules/nf-core/deeptools/plotprofile/main.nf @@ -2,7 +2,7 @@ process DEEPTOOLS_PLOTPROFILE { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? 'bioconda::deeptools=3.5.1' : null) + conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" diff --git a/modules/nf-core/deeptools/plotprofile/meta.yml b/modules/nf-core/deeptools/plotprofile/meta.yml index 795fda44..44612ea9 100644 --- a/modules/nf-core/deeptools/plotprofile/meta.yml +++ b/modules/nf-core/deeptools/plotprofile/meta.yml @@ -8,7 +8,6 @@ keywords: tools: - deeptools: description: A set of user-friendly tools for normalization and visualization of deep-sequencing data - homepage: documentation: https://deeptools.readthedocs.io/en/develop/index.html tool_dev_url: https://github.com/deeptools/deepTools doi: "10.1093/nar/gku365" diff --git a/modules/nf-core/gffread/main.nf b/modules/nf-core/gffread/main.nf index 7c575c97..b1a8996f 100644 --- a/modules/nf-core/gffread/main.nf +++ b/modules/nf-core/gffread/main.nf @@ -2,7 +2,7 @@ process GFFREAD { tag "$gff" label 'process_low' - conda (params.enable_conda ? "bioconda::gffread=0.12.1" : null) + conda "bioconda::gffread=0.12.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0' : 'quay.io/biocontainers/gffread:0.12.1--h8b12597_0' }" diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf index fa6ba26a..d906034c 100644 --- a/modules/nf-core/gunzip/main.nf +++ b/modules/nf-core/gunzip/main.nf @@ -2,7 +2,7 @@ process GUNZIP { tag "$archive" label 'process_single' - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'ubuntu:20.04' }" diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml index 4d2ebc84..2e0e4054 100644 --- a/modules/nf-core/gunzip/meta.yml +++ b/modules/nf-core/gunzip/meta.yml @@ -5,29 +5,29 @@ keywords: - compression tools: - gunzip: - description: | - gzip is a file format and a software application used for file compression and decompression. - documentation: https://www.gnu.org/software/gzip/manual/gzip.html - licence: ["GPL-3.0-or-later"] + description: | + gzip is a file format and a software application used for file compression and decompression. + documentation: https://www.gnu.org/software/gzip/manual/gzip.html + licence: ["GPL-3.0-or-later"] input: - meta: - type: map - description: | - Optional groovy Map containing meta information - e.g. [ id:'test', single_end:false ] + type: map + description: | + Optional groovy Map containing meta information + e.g. [ id:'test', single_end:false ] - archive: - type: file - description: File to be compressed/uncompressed - pattern: "*.*" + type: file + description: File to be compressed/uncompressed + pattern: "*.*" output: - gunzip: - type: file - description: Compressed/uncompressed file - pattern: "*.*" + type: file + description: Compressed/uncompressed file + pattern: "*.*" - versions: - type: file - description: File containing software versions - pattern: "versions.yml" + type: file + description: File containing software versions + pattern: "versions.yml" authors: - "@joseespinosa" - "@drpatelh" diff --git a/modules/nf-core/homer/annotatepeaks/main.nf b/modules/nf-core/homer/annotatepeaks/main.nf index 9056a5ab..865ad81c 100644 --- a/modules/nf-core/homer/annotatepeaks/main.nf +++ b/modules/nf-core/homer/annotatepeaks/main.nf @@ -3,7 +3,7 @@ process HOMER_ANNOTATEPEAKS { label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda (params.enable_conda ? "bioconda::homer=4.11" : null) + conda "bioconda::homer=4.11" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/homer:4.11--pl526hc9558a2_3' : 'quay.io/biocontainers/homer:4.11--pl526hc9558a2_3' }" diff --git a/modules/nf-core/khmer/uniquekmers/main.nf b/modules/nf-core/khmer/uniquekmers/main.nf index 8ad89a62..02746ae1 100644 --- a/modules/nf-core/khmer/uniquekmers/main.nf +++ b/modules/nf-core/khmer/uniquekmers/main.nf @@ -2,7 +2,7 @@ process KHMER_UNIQUEKMERS { tag "$fasta" label 'process_low' - conda (params.enable_conda ? "bioconda::khmer=3.0.0a3" : null) + conda "bioconda::khmer=3.0.0a3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/khmer:3.0.0a3--py37haa7609a_2' : 'quay.io/biocontainers/khmer:3.0.0a3--py37haa7609a_2' }" diff --git a/modules/nf-core/khmer/uniquekmers/meta.yml b/modules/nf-core/khmer/uniquekmers/meta.yml index 31405cc1..f9f63972 100644 --- a/modules/nf-core/khmer/uniquekmers/meta.yml +++ b/modules/nf-core/khmer/uniquekmers/meta.yml @@ -1,5 +1,5 @@ name: "khmer_uniquekmers" -description: +description: In-memory nucleotide sequence k-mer counting, filtering, graph traversal and more keywords: - khmer - k-mer diff --git a/modules/nf-core/macs2/callpeak/main.nf b/modules/nf-core/macs2/callpeak/main.nf index 9aaf97a9..feca0425 100644 --- a/modules/nf-core/macs2/callpeak/main.nf +++ b/modules/nf-core/macs2/callpeak/main.nf @@ -2,7 +2,7 @@ process MACS2_CALLPEAK { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::macs2=2.2.7.1" : null) + conda "bioconda::macs2=2.2.7.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/macs2:2.2.7.1--py38h4a8c8d9_3' : 'quay.io/biocontainers/macs2:2.2.7.1--py38h4a8c8d9_3' }" diff --git a/modules/nf-core/macs2/callpeak/meta.yml b/modules/nf-core/macs2/callpeak/meta.yml index 982bc5b2..6e2bc1db 100644 --- a/modules/nf-core/macs2/callpeak/meta.yml +++ b/modules/nf-core/macs2/callpeak/meta.yml @@ -8,10 +8,10 @@ keywords: tools: - macs2: description: Model Based Analysis for ChIP-Seq data - homepage: None + documentation: https://docs.csc.fi/apps/macs2/ tool_dev_url: https://github.com/macs3-project/MACS - doi: "https://doi.org/10.1101/496521" + doi: "10.1101/496521" licence: ["BSD"] input: diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 68f66bea..4b604749 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.13" + conda "bioconda::multiqc=1.14" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/phantompeakqualtools/main.nf b/modules/nf-core/phantompeakqualtools/main.nf index 43fca683..27daac4c 100644 --- a/modules/nf-core/phantompeakqualtools/main.nf +++ b/modules/nf-core/phantompeakqualtools/main.nf @@ -3,7 +3,7 @@ process PHANTOMPEAKQUALTOOLS { label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda (params.enable_conda ? "bioconda::phantompeakqualtools=1.2.2" : null) + conda "bioconda::phantompeakqualtools=1.2.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/phantompeakqualtools:1.2.2--0' : 'quay.io/biocontainers/phantompeakqualtools:1.2.2--0' }" diff --git a/modules/nf-core/phantompeakqualtools/meta.yml b/modules/nf-core/phantompeakqualtools/meta.yml index 6488500d..c8290fbd 100644 --- a/modules/nf-core/phantompeakqualtools/meta.yml +++ b/modules/nf-core/phantompeakqualtools/meta.yml @@ -1,6 +1,10 @@ name: "phantompeakqualtools" -description: +description: | + "This package computes informative enrichment and quality measures + for ChIP-seq/DNase-seq/FAIRE-seq/MNase-seq data. It can also be used + to obtain robust estimates of the predominant fragment length or + characteristic tag shift values in these assays." keywords: - "ChIP-Seq" - "QC" @@ -12,10 +16,10 @@ tools: for ChIP-seq/DNase-seq/FAIRE-seq/MNase-seq data. It can also be used to obtain robust estimates of the predominant fragment length or characteristic tag shift values in these assays." - homepage: "None" + documentation: "https://github.com/kundajelab/phantompeakqualtools" tool_dev_url: "https://github.com/kundajelab/phantompeakqualtools" - doi: "https://doi.org/10.1101/gr.136184.111" + doi: "10.1101/gr.136184.111" licence: "['BSD-3-clause']" input: diff --git a/modules/nf-core/picard/markduplicates/main.nf b/modules/nf-core/picard/markduplicates/main.nf index 4e559fea..1fe6ee2d 100644 --- a/modules/nf-core/picard/markduplicates/main.nf +++ b/modules/nf-core/picard/markduplicates/main.nf @@ -2,13 +2,15 @@ process PICARD_MARKDUPLICATES { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::picard=2.27.4" : null) + conda "bioconda::picard=3.0.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:2.27.4--hdfd78af_0' : - 'quay.io/biocontainers/picard:2.27.4--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : + 'quay.io/biocontainers/picard:3.0.0--hdfd78af_1' }" input: tuple val(meta), path(bam) + path fasta + path fai output: tuple val(meta), path("*.bam") , emit: bam @@ -22,19 +24,20 @@ process PICARD_MARKDUPLICATES { script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def avail_mem = 3 + def avail_mem = 3072 if (!task.memory) { log.info '[Picard MarkDuplicates] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { - avail_mem = task.memory.giga + avail_mem = (task.memory.mega*0.8).intValue() } """ picard \\ - -Xmx${avail_mem}g \\ + -Xmx${avail_mem}M \\ MarkDuplicates \\ $args \\ --INPUT $bam \\ --OUTPUT ${prefix}.bam \\ + --REFERENCE_SEQUENCE $fasta \\ --METRICS_FILE ${prefix}.MarkDuplicates.metrics.txt cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/picard/markduplicates/meta.yml b/modules/nf-core/picard/markduplicates/meta.yml index 842817bc..3f2357bb 100644 --- a/modules/nf-core/picard/markduplicates/meta.yml +++ b/modules/nf-core/picard/markduplicates/meta.yml @@ -24,7 +24,15 @@ input: - bam: type: file description: BAM file - pattern: "*.{bam}" + pattern: "*.{bam,cram,sam}" + - fasta: + type: file + description: Reference genome fasta file + pattern: "*.{fasta,fa}" + - fai: + type: file + description: Reference genome fasta index + pattern: "*.{fai}" output: - meta: type: map diff --git a/modules/nf-core/picard/mergesamfiles/main.nf b/modules/nf-core/picard/mergesamfiles/main.nf index cccf4d3d..ff8d2f17 100644 --- a/modules/nf-core/picard/mergesamfiles/main.nf +++ b/modules/nf-core/picard/mergesamfiles/main.nf @@ -2,10 +2,10 @@ process PICARD_MERGESAMFILES { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::picard=2.27.4" : null) + conda "bioconda::picard=3.0.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:2.27.4--hdfd78af_0' : - 'quay.io/biocontainers/picard:2.27.4--hdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : + 'quay.io/biocontainers/picard:3.0.0--hdfd78af_1' }" input: tuple val(meta), path(bams) @@ -21,16 +21,16 @@ process PICARD_MERGESAMFILES { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def bam_files = bams.sort() - def avail_mem = 3 + def avail_mem = 3072 if (!task.memory) { log.info '[Picard MergeSamFiles] Available memory not known - defaulting to 3GB. Specify process memory requirements to change this.' } else { - avail_mem = task.memory.giga + avail_mem = (task.memory.mega*0.8).intValue() } if (bam_files.size() > 1) { """ picard \\ - -Xmx${avail_mem}g \\ + -Xmx${avail_mem}M \\ MergeSamFiles \\ $args \\ ${'--INPUT '+bam_files.join(' --INPUT ')} \\ diff --git a/modules/nf-core/preseq/lcextrap/main.nf b/modules/nf-core/preseq/lcextrap/main.nf index a98a922c..38275910 100644 --- a/modules/nf-core/preseq/lcextrap/main.nf +++ b/modules/nf-core/preseq/lcextrap/main.nf @@ -3,7 +3,7 @@ process PRESEQ_LCEXTRAP { label 'process_single' label 'error_ignore' - conda (params.enable_conda ? "bioconda::preseq=3.1.2" : null) + conda "bioconda::preseq=3.1.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/preseq:3.1.2--h445547b_2': 'quay.io/biocontainers/preseq:3.1.2--h445547b_2' }" diff --git a/modules/nf-core/preseq/lcextrap/meta.yml b/modules/nf-core/preseq/lcextrap/meta.yml index f1be05a2..1391961c 100755 --- a/modules/nf-core/preseq/lcextrap/meta.yml +++ b/modules/nf-core/preseq/lcextrap/meta.yml @@ -10,7 +10,7 @@ tools: homepage: http://smithlabresearch.org/software/preseq/ documentation: http://smithlabresearch.org/wp-content/uploads/manual.pdf tool_dev_url: https://github.com/smithlabcode/preseq - doi: "" + licence: ["GPL"] input: diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf index c3152aca..2120cd7d 100644 --- a/modules/nf-core/samtools/flagstat/main.nf +++ b/modules/nf-core/samtools/flagstat/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT { tag "$meta.id" label 'process_single' - conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : + 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/samtools/flagstat/meta.yml b/modules/nf-core/samtools/flagstat/meta.yml index 95269063..954225df 100644 --- a/modules/nf-core/samtools/flagstat/meta.yml +++ b/modules/nf-core/samtools/flagstat/meta.yml @@ -14,7 +14,7 @@ tools: short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA. homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html + documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] input: diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf index 87618e5f..a7b87d8b 100644 --- a/modules/nf-core/samtools/idxstats/main.nf +++ b/modules/nf-core/samtools/idxstats/main.nf @@ -1,11 +1,11 @@ process SAMTOOLS_IDXSTATS { tag "$meta.id" - label 'process_low' + label 'process_single' - conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : + 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/samtools/idxstats/meta.yml b/modules/nf-core/samtools/idxstats/meta.yml index 3710ab88..dda87e1e 100644 --- a/modules/nf-core/samtools/idxstats/meta.yml +++ b/modules/nf-core/samtools/idxstats/meta.yml @@ -15,7 +15,7 @@ tools: short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA. homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html + documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] input: diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index e04e63e8..8b95687a 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : + 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml index e5cadbc2..8bd2fa6f 100644 --- a/modules/nf-core/samtools/index/meta.yml +++ b/modules/nf-core/samtools/index/meta.yml @@ -12,7 +12,7 @@ tools: short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA. homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html + documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] input: diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf index ab7f1cca..84c167cd 100644 --- a/modules/nf-core/samtools/sort/main.nf +++ b/modules/nf-core/samtools/sort/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_SORT { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : + 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml index 09289751..07328431 100644 --- a/modules/nf-core/samtools/sort/meta.yml +++ b/modules/nf-core/samtools/sort/meta.yml @@ -12,7 +12,7 @@ tools: short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA. homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html + documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] input: diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf index 9b0c3867..0a2a3640 100644 --- a/modules/nf-core/samtools/stats/main.nf +++ b/modules/nf-core/samtools/stats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_STATS { tag "$meta.id" label 'process_single' - conda (params.enable_conda ? "bioconda::samtools=1.15.1" : null) + conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.15.1--h1170115_0' : - 'quay.io/biocontainers/samtools:1.15.1--h1170115_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : + 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" input: tuple val(meta), path(input), path(input_index) diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml index cac50b1c..1d68a5d8 100644 --- a/modules/nf-core/samtools/stats/meta.yml +++ b/modules/nf-core/samtools/stats/meta.yml @@ -13,7 +13,7 @@ tools: short DNA sequence read alignments in the SAM, BAM and CRAM formats, written by Heng Li. These files are generated as output by short read aligners like BWA. homepage: http://www.htslib.org/ - documentation: hhttp://www.htslib.org/doc/samtools.html + documentation: http://www.htslib.org/doc/samtools.html doi: 10.1093/bioinformatics/btp352 licence: ["MIT"] input: @@ -23,13 +23,13 @@ input: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - input: - type: file - description: BAM/CRAM file from alignment - pattern: "*.{bam,cram}" + type: file + description: BAM/CRAM file from alignment + pattern: "*.{bam,cram}" - input_index: - type: file - description: BAI/CRAI file from alignment - pattern: "*.{bai,crai}" + type: file + description: BAI/CRAI file from alignment + pattern: "*.{bai,crai}" - fasta: type: optional file description: Reference file the CRAM was created with diff --git a/modules/nf-core/subread/featurecounts/main.nf b/modules/nf-core/subread/featurecounts/main.nf index 18e2a92b..cbc833fe 100644 --- a/modules/nf-core/subread/featurecounts/main.nf +++ b/modules/nf-core/subread/featurecounts/main.nf @@ -2,7 +2,7 @@ process SUBREAD_FEATURECOUNTS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::subread=2.0.1" : null) + conda "bioconda::subread=2.0.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' : 'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }" diff --git a/modules/nf-core/trimgalore/main.nf b/modules/nf-core/trimgalore/main.nf index a69e3de6..dc295784 100644 --- a/modules/nf-core/trimgalore/main.nf +++ b/modules/nf-core/trimgalore/main.nf @@ -2,7 +2,7 @@ process TRIMGALORE { tag "$meta.id" label 'process_high' - conda (params.enable_conda ? 'bioconda::trim-galore=0.6.7' : null) + conda "bioconda::trim-galore=0.6.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/trim-galore:0.6.7--hdfd78af_0' : 'quay.io/biocontainers/trim-galore:0.6.7--hdfd78af_0' }" @@ -11,13 +11,12 @@ process TRIMGALORE { tuple val(meta), path(reads) output: - tuple val(meta), path("*{trimmed,val}*.fq.gz"), emit: reads - tuple val(meta), path("*report.txt") , emit: log - path "versions.yml" , emit: versions - - tuple val(meta), path("*unpaired*.fq.gz") , emit: unpaired, optional: true - tuple val(meta), path("*.html") , emit: html , optional: true - tuple val(meta), path("*.zip") , emit: zip , optional: true + tuple val(meta), path("*{3prime,5prime,trimmed,val}*.fq.gz"), emit: reads + tuple val(meta), path("*report.txt") , emit: log , optional: true + tuple val(meta), path("*unpaired*.fq.gz") , emit: unpaired, optional: true + tuple val(meta), path("*.html") , emit: html , optional: true + tuple val(meta), path("*.zip") , emit: zip , optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -35,23 +34,17 @@ process TRIMGALORE { if (cores > 8) cores = 8 } - // Clipping presets have to be evaluated in the context of SE/PE - def c_r1 = params.clip_r1 > 0 ? "--clip_r1 ${params.clip_r1}" : '' - def c_r2 = params.clip_r2 > 0 ? "--clip_r2 ${params.clip_r2}" : '' - def tpc_r1 = params.three_prime_clip_r1 > 0 ? "--three_prime_clip_r1 ${params.three_prime_clip_r1}" : '' - def tpc_r2 = params.three_prime_clip_r2 > 0 ? "--three_prime_clip_r2 ${params.three_prime_clip_r2}" : '' - // Added soft-links to original fastqs for consistent naming in MultiQC def prefix = task.ext.prefix ?: "${meta.id}" if (meta.single_end) { + def args_list = args.split("\\s(?=--)").toList() + args_list.removeAll { it.toLowerCase().contains('_r2 ') } """ [ ! -f ${prefix}.fastq.gz ] && ln -s $reads ${prefix}.fastq.gz trim_galore \\ - $args \\ + ${args_list.join(' ')} \\ --cores $cores \\ --gzip \\ - $c_r1 \\ - $tpc_r1 \\ ${prefix}.fastq.gz cat <<-END_VERSIONS > versions.yml @@ -69,10 +62,6 @@ process TRIMGALORE { --cores $cores \\ --paired \\ --gzip \\ - $c_r1 \\ - $c_r2 \\ - $tpc_r1 \\ - $tpc_r2 \\ ${prefix}_1.fastq.gz \\ ${prefix}_2.fastq.gz diff --git a/modules/nf-core/trimgalore/meta.yml b/modules/nf-core/trimgalore/meta.yml index 439f566d..f84c4d77 100644 --- a/modules/nf-core/trimgalore/meta.yml +++ b/modules/nf-core/trimgalore/meta.yml @@ -36,7 +36,7 @@ output: description: | List of input adapter trimmed FastQ files of size 1 and 2 for single-end and paired-end data, respectively. - pattern: "*.{fq.gz}" + pattern: "*{3prime,5prime,trimmed,val}*.fq.gz" - unpaired: type: file description: | diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf index a2979e04..defda3ef 100644 --- a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf +++ b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf @@ -3,7 +3,7 @@ process UCSC_BEDGRAPHTOBIGWIG { label 'process_single' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda (params.enable_conda ? "bioconda::ucsc-bedgraphtobigwig=377" : null) + conda "bioconda::ucsc-bedgraphtobigwig=377" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' : 'quay.io/biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }" diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml index 1be1a3b7..ba8915be 100755 --- a/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml +++ b/modules/nf-core/ucsc/bedgraphtobigwig/meta.yml @@ -6,10 +6,8 @@ keywords: tools: - ucsc: description: Convert a bedGraph file to bigWig format. - homepage: None - documentation: None - tool_dev_url: None - doi: "" + homepage: http://hgdownload.cse.ucsc.edu/admin/exe/ + documentation: https://genome.ucsc.edu/goldenPath/help/bigWig.html licence: ["varies; see http://genome.ucsc.edu/license"] input: From 8daf9569dce8d2e2e7858d77ee4c2b392beb005d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 21 Apr 2023 13:55:13 +0200 Subject: [PATCH 055/201] Install bam_markduplicates_picard from nf-core repo --- modules.json | 11 +++- .../nf-core/bam_markduplicates_picard/main.nf | 52 ++++++++++++++++ .../bam_markduplicates_picard/meta.yml | 60 +++++++++++++++++++ .../nf-core/mark_duplicates_picard.nf | 42 ------------- workflows/chipseq.nf | 22 +++---- 5 files changed, 132 insertions(+), 55 deletions(-) create mode 100644 subworkflows/nf-core/bam_markduplicates_picard/main.nf create mode 100644 subworkflows/nf-core/bam_markduplicates_picard/meta.yml delete mode 100644 subworkflows/nf-core/mark_duplicates_picard.nf diff --git a/modules.json b/modules.json index d6769afc..48c56fc0 100644 --- a/modules.json +++ b/modules.json @@ -113,7 +113,7 @@ "picard/markduplicates": { "branch": "master", "git_sha": "75027bf77472b1f4fd2cdd7e46f83119dfb0f2c6", - "installed_by": ["modules"] + "installed_by": ["modules", "bam_markduplicates_picard"] }, "picard/mergesamfiles": { "branch": "master", @@ -138,7 +138,7 @@ "samtools/index": { "branch": "master", "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["bam_sort_stats_samtools", "modules"] + "installed_by": ["modules", "bam_markduplicates_picard", "bam_sort_stats_samtools"] }, "samtools/sort": { "branch": "master", @@ -179,6 +179,11 @@ }, "subworkflows": { "nf-core": { + "bam_markduplicates_picard": { + "branch": "master", + "git_sha": "6f1697c121719dedde9e0537b6ed6a9cb8c13583", + "installed_by": ["subworkflows"] + }, "bam_sort_stats_samtools": { "branch": "master", "git_sha": "3911652a6b24249358f79e8b8466338d63efb2a2", @@ -187,7 +192,7 @@ "bam_stats_samtools": { "branch": "master", "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e", - "installed_by": ["bam_sort_stats_samtools"] + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] }, "fastq_align_bowtie2": { "branch": "master", diff --git a/subworkflows/nf-core/bam_markduplicates_picard/main.nf b/subworkflows/nf-core/bam_markduplicates_picard/main.nf new file mode 100644 index 00000000..6e3df332 --- /dev/null +++ b/subworkflows/nf-core/bam_markduplicates_picard/main.nf @@ -0,0 +1,52 @@ +// +// Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +// + +include { PICARD_MARKDUPLICATES } from '../../../modules/nf-core/picard/markduplicates/main' +include { SAMTOOLS_INDEX } from '../../../modules/nf-core/samtools/index/main' +include { BAM_STATS_SAMTOOLS } from '../bam_stats_samtools/main' + +workflow BAM_MARKDUPLICATES_PICARD { + + take: + ch_bam // channel: [ val(meta), path(bam) ] + ch_fasta // channel: [ path(fasta) ] + ch_fai // channel: [ path(fai) ] + + main: + + ch_versions = Channel.empty() + + PICARD_MARKDUPLICATES ( ch_bam, ch_fasta, ch_fai ) + ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions.first()) + + SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) + ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) + + ch_bam_bai = PICARD_MARKDUPLICATES.out.bam + .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) + .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) + .map { + meta, bam, bai, csi -> + if (bai) { + [ meta, bam, bai ] + } else { + [ meta, bam, csi ] + } + } + + BAM_STATS_SAMTOOLS ( ch_bam_bai, ch_fasta ) + ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions) + + emit: + bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), path(bam) ] + metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), path(bam) ] + bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), path(bai) ] + csi = SAMTOOLS_INDEX.out.csi // channel: [ val(meta), path(csi) ] + + stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), path(stats) ] + flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), path(flagstat) ] + idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), path(idxstats) ] + + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/subworkflows/nf-core/bam_markduplicates_picard/meta.yml b/subworkflows/nf-core/bam_markduplicates_picard/meta.yml new file mode 100644 index 00000000..822c6132 --- /dev/null +++ b/subworkflows/nf-core/bam_markduplicates_picard/meta.yml @@ -0,0 +1,60 @@ +name: "bam_markduplicates_picard" +description: Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats +keywords: + - markduplicates + - bam + - sam + - cram + +modules: + - picard/markduplicates + - samtools/index + - samtools/stats + - samtools/idxstats + - samtools/flagstat + +input: + - ch_bam: + description: | + BAM/CRAM/SAM file + Structure: [ val(meta), path(bam) ] + - ch_fasta: + description: | + Reference genome fasta file + Structure: [ path(fasta) ] + - ch_fasta: + description: | + Index of the reference genome fasta file + Structure: [ path(fai) ] +output: + - bam: + description: | + processed BAM/CRAM/SAM file + Structure: [ val(meta), path(bam) ] + - bai: + description: | + BAM/CRAM/SAM samtools index + Structure: [ val(meta), path(bai) ] + - csi: + description: | + CSI samtools index + Structure: [ val(meta), path(csi) ] + - stats: + description: | + File containing samtools stats output + Structure: [ val(meta), path(stats) ] + - flagstat: + description: | + File containing samtools flagstat output + Structure: [ val(meta), path(flagstat) ] + - idxstats: + description: | + File containing samtools idxstats output + Structure: [ val(meta), path(idxstats) ] + - versions: + description: | + Files containing software versions + Structure: [ path(versions.yml) ] +authors: + - "@dmarron" + - "@drpatelh" diff --git a/subworkflows/nf-core/mark_duplicates_picard.nf b/subworkflows/nf-core/mark_duplicates_picard.nf deleted file mode 100644 index 72a0eb8e..00000000 --- a/subworkflows/nf-core/mark_duplicates_picard.nf +++ /dev/null @@ -1,42 +0,0 @@ -/* - * Picard MarkDuplicates, sort, index BAM file and run samtools stats, flagstat and idxstats - */ - -include { PICARD_MARKDUPLICATES } from '../../modules/nf-core/picard/markduplicates/main' -include { SAMTOOLS_INDEX } from '../../modules/nf-core/samtools/index/main' -include { BAM_STATS_SAMTOOLS } from './bam_stats_samtools' - -workflow MARK_DUPLICATES_PICARD { - take: - bam // channel: [ val(meta), [ bam ] ] - - main: - - ch_versions = Channel.empty() - - // - // Picard MarkDuplicates - // - PICARD_MARKDUPLICATES(bam) - ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions.first()) - - // - // Index BAM file and run samtools stats, flagstat and idxstats - // - SAMTOOLS_INDEX(PICARD_MARKDUPLICATES.out.bam) - ch_versions = ch_versions.mix(SAMTOOLS_INDEX.out.versions.first()) - - BAM_STATS_SAMTOOLS(PICARD_MARKDUPLICATES.out.bam.join(SAMTOOLS_INDEX.out.bai, by: [0])) - ch_versions = ch_versions.mix(BAM_STATS_SAMTOOLS.out.versions) - - emit: - bam = PICARD_MARKDUPLICATES.out.bam // channel: [ val(meta), [ bam ] ] - metrics = PICARD_MARKDUPLICATES.out.metrics // channel: [ val(meta), [ metrics ] ] - - bai = SAMTOOLS_INDEX.out.bai // channel: [ val(meta), [ bai ] ] - stats = BAM_STATS_SAMTOOLS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = BAM_STATS_SAMTOOLS.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = BAM_STATS_SAMTOOLS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // channel: [ versions.yml ] -} diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 71d7dfe8..bdf5783f 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -120,7 +120,7 @@ include { FASTQ_FASTQC_UMITOOLS_TRIMGALORE } from '../subworkflows/nf-core/fastq include { FASTQ_ALIGN_BWA } from '../subworkflows/nf-core/fastq_align_bwa/main' include { FASTQ_ALIGN_BOWTIE2 } from '../subworkflows/nf-core/fastq_align_bowtie2/main' include { FASTQ_ALIGN_CHROMAP } from '../subworkflows/nf-core/fastq_align_chromap/main' -include { MARK_DUPLICATES_PICARD } from '../subworkflows/nf-core/mark_duplicates_picard' +include { BAM_MARKDUPLICATES_PICARD } from '../subworkflows/nf-core/bam_markduplicates_picard/main' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -279,16 +279,18 @@ workflow CHIPSEQ { // // SUBWORKFLOW: Mark duplicates & filter BAM files after merging // - MARK_DUPLICATES_PICARD ( - PICARD_MERGESAMFILES.out.bam + BAM_MARKDUPLICATES_PICARD ( + PICARD_MERGESAMFILES.out.bam, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai ) - ch_versions = ch_versions.mix(MARK_DUPLICATES_PICARD.out.versions) + ch_versions = ch_versions.mix(BAM_MARKDUPLICATES_PICARD.out.versions) // // SUBWORKFLOW: Filter BAM file with BamTools // BAM_FILTER_BAMTOOLS ( - MARK_DUPLICATES_PICARD.out.bam.join(MARK_DUPLICATES_PICARD.out.bai, by: [0]), + BAM_MARKDUPLICATES_PICARD.out.bam.join(BAM_MARKDUPLICATES_PICARD.out.bai, by: [0]), PREPARE_GENOME.out.filtered_bed.first(), PREPARE_GENOME.out.fasta, ch_bamtools_filter_se_config, @@ -302,7 +304,7 @@ workflow CHIPSEQ { ch_preseq_multiqc = Channel.empty() if (!params.skip_preseq) { PRESEQ_LCEXTRAP ( - MARK_DUPLICATES_PICARD.out.bam + BAM_MARKDUPLICATES_PICARD.out.bam ) ch_preseq_multiqc = PRESEQ_LCEXTRAP.out.lc_extrap ch_versions = ch_versions.mix(PRESEQ_LCEXTRAP.out.versions.first()) @@ -713,10 +715,10 @@ workflow CHIPSEQ { ch_samtools_flagstat.collect{it[1]}.ifEmpty([]), ch_samtools_idxstats.collect{it[1]}.ifEmpty([]), - MARK_DUPLICATES_PICARD.out.stats.collect{it[1]}.ifEmpty([]), - MARK_DUPLICATES_PICARD.out.flagstat.collect{it[1]}.ifEmpty([]), - MARK_DUPLICATES_PICARD.out.idxstats.collect{it[1]}.ifEmpty([]), - MARK_DUPLICATES_PICARD.out.metrics.collect{it[1]}.ifEmpty([]), + BAM_MARKDUPLICATES_PICARD.out.stats.collect{it[1]}.ifEmpty([]), + BAM_MARKDUPLICATES_PICARD.out.flagstat.collect{it[1]}.ifEmpty([]), + BAM_MARKDUPLICATES_PICARD.out.idxstats.collect{it[1]}.ifEmpty([]), + BAM_MARKDUPLICATES_PICARD.out.metrics.collect{it[1]}.ifEmpty([]), BAM_FILTER_BAMTOOLS.out.stats.collect{it[1]}.ifEmpty([]), BAM_FILTER_BAMTOOLS.out.flagstat.collect{it[1]}.ifEmpty([]), From 73919ee93e119e7b5685a3dd4d24a04a42ed6ca7 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 21 Apr 2023 14:04:31 +0200 Subject: [PATCH 056/201] Also update instances of bam_markduplicates_picard in modules --- conf/modules.config | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index dfc0c5ec..0381c658 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -322,7 +322,7 @@ process { publishDir = [ enabled: false ] } - withName: '.*:MARK_DUPLICATES_PICARD:PICARD_MARKDUPLICATES' { + withName: '.*:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES' { ext.args = '--ASSUME_SORTED true --REMOVE_DUPLICATES false --VALIDATION_STRINGENCY LENIENT --TMP_DIR tmp' ext.prefix = { "${meta.id}.mLb.mkD.sorted" } publishDir = [ @@ -340,7 +340,7 @@ process { ] } - withName: '.*:MARK_DUPLICATES_PICARD:SAMTOOLS_INDEX' { + withName: '.*:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX' { publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, mode: params.publish_dir_mode, @@ -349,7 +349,7 @@ process { ] } - withName: '.*:MARK_DUPLICATES_PICARD:BAM_STATS_SAMTOOLS:.*' { + withName: '.*:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:.*' { ext.prefix = { "${meta.id}.mLb.mkD.sorted.bam" } publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary/samtools_stats" }, From 477491d9376969a4db549d2a049533a653251d5a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 21 Apr 2023 14:08:10 +0200 Subject: [PATCH 057/201] Remove obsolet subworkflow --- subworkflows/nf-core/bam_stats_samtools.nf | 32 ---------------------- 1 file changed, 32 deletions(-) delete mode 100644 subworkflows/nf-core/bam_stats_samtools.nf diff --git a/subworkflows/nf-core/bam_stats_samtools.nf b/subworkflows/nf-core/bam_stats_samtools.nf deleted file mode 100644 index 207c5ece..00000000 --- a/subworkflows/nf-core/bam_stats_samtools.nf +++ /dev/null @@ -1,32 +0,0 @@ -/* - * Run SAMtools stats, flagstat and idxstats - */ - -include { SAMTOOLS_STATS } from '../../modules/nf-core/samtools/stats/main' -include { SAMTOOLS_IDXSTATS } from '../../modules/nf-core/samtools/idxstats/main' -include { SAMTOOLS_FLAGSTAT } from '../../modules/nf-core/samtools/flagstat/main' - -workflow BAM_STATS_SAMTOOLS { - take: - ch_bam_bai // channel: [ val(meta), [ bam ], [bai] ] - - main: - - ch_versions = Channel.empty() - - SAMTOOLS_STATS ( ch_bam_bai, [] ) - ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions.first()) - - SAMTOOLS_FLAGSTAT ( ch_bam_bai ) - ch_versions = ch_versions.mix(SAMTOOLS_FLAGSTAT.out.versions.first()) - - SAMTOOLS_IDXSTATS ( ch_bam_bai ) - ch_versions = ch_versions.mix(SAMTOOLS_IDXSTATS.out.versions.first()) - - emit: - stats = SAMTOOLS_STATS.out.stats // channel: [ val(meta), [ stats ] ] - flagstat = SAMTOOLS_FLAGSTAT.out.flagstat // channel: [ val(meta), [ flagstat ] ] - idxstats = SAMTOOLS_IDXSTATS.out.idxstats // channel: [ val(meta), [ idxstats ] ] - - versions = ch_versions // channel: [ versions.yml ] -} From 6c32d4e62cad615f9fa98be7a0ce4d310285d4e7 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 21 Apr 2023 14:08:24 +0200 Subject: [PATCH 058/201] Fix bwa_index --- subworkflows/local/prepare_genome.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 4cb2d164..3a42267e 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -144,7 +144,7 @@ workflow PREPARE_GENOME { ch_bwa_index = file(params.bwa_index) } } else { - ch_bwa_index = BWA_INDEX ( ch_fasta ).index + ch_bwa_index = BWA_INDEX ( [ [:], ch_fasta ] ).index ch_versions = ch_versions.mix(BWA_INDEX.out.versions) } } From 59c02c5817c6b8d99070c46b81cbc0b0abf3ae74 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 24 Apr 2023 12:14:02 +0200 Subject: [PATCH 059/201] Update changelog --- CHANGELOG.md | 15 +++++++++++++++ 1 file changed, 15 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index b0266c0d..86bffe83 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -16,6 +16,21 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#320](https://github.com/nf-core/chipseq/issues/320)] Fix samplesheet control column in documentation examples. - [[#328](https://github.com/nf-core/chipseq/issues/328)] Modify documentation to clarify that is necessary to provide the `--read_length` when `--genome` is set and `--macs_gsize` has not provided. +### Software dependencies + +Note, since the pipeline is now using Nextflow DSL2, each process will be run with its own [Biocontainer](https://biocontainers.pro/#/registry). This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference. + +| Dependency | Old version | New version | +| ----------------------- | ----------- | ----------- | +| `chromap` | 0.2.1 | 0.2.4 | +| `multiqc` | 1.13 | 1.14 | +| `picard` | 2.27.4 | 3.0.0 | +| `samtools` | 1.15.1 | 1.16.1 | + +> **NB:** Dependency has been **updated** if both old and new version information is present. +> **NB:** Dependency has been **added** if just the new version information is present. +> **NB:** Dependency has been **removed** if version information isn't present. + ## [[2.0.0](https://github.com/nf-core/chipseq/releases/tag/2.0.0)] - 2022-10-03 ### Enhancements & fixes From fa049414417ba1965f26b50e99d69a9b26a72751 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 24 Apr 2023 15:53:01 +0200 Subject: [PATCH 060/201] Make prettier happy --- CHANGELOG.md | 12 ++++++------ 1 file changed, 6 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 86bffe83..a2ed9aaa 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -20,12 +20,12 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 Note, since the pipeline is now using Nextflow DSL2, each process will be run with its own [Biocontainer](https://biocontainers.pro/#/registry). This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference. -| Dependency | Old version | New version | -| ----------------------- | ----------- | ----------- | -| `chromap` | 0.2.1 | 0.2.4 | -| `multiqc` | 1.13 | 1.14 | -| `picard` | 2.27.4 | 3.0.0 | -| `samtools` | 1.15.1 | 1.16.1 | +| Dependency | Old version | New version | +| ---------- | ----------- | ----------- | +| `chromap` | 0.2.1 | 0.2.4 | +| `multiqc` | 1.13 | 1.14 | +| `picard` | 2.27.4 | 3.0.0 | +| `samtools` | 1.15.1 | 1.16.1 | > **NB:** Dependency has been **updated** if both old and new version information is present. > **NB:** Dependency has been **added** if just the new version information is present. From 02d021b0253e4a5bafcf24c434bc3b52ea7cbc6d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 25 Apr 2023 20:02:09 +0200 Subject: [PATCH 061/201] Remove enable_conda from local modules --- modules/local/annotate_boolean_peaks.nf | 2 +- modules/local/bam_remove_orphans.nf | 2 +- modules/local/bamtools_filter.nf | 2 +- modules/local/bedtools_genomecov.nf | 2 +- modules/local/deseq2_qc.nf | 2 +- modules/local/frip_score.nf | 2 +- modules/local/genome_blacklist_regions.nf | 2 +- modules/local/gtf2bed.nf | 2 +- modules/local/igv.nf | 2 +- modules/local/macs2_consensus.nf | 2 +- modules/local/multiqc.nf | 2 +- modules/local/multiqc_custom_peaks.nf | 2 +- modules/local/multiqc_custom_phantompeakqualtools.nf | 2 +- modules/local/plot_homer_annotatepeaks.nf | 2 +- modules/local/plot_macs2_qc.nf | 2 +- modules/local/star_align.nf | 2 +- modules/local/star_genomegenerate.nf | 2 +- 17 files changed, 17 insertions(+), 17 deletions(-) diff --git a/modules/local/annotate_boolean_peaks.nf b/modules/local/annotate_boolean_peaks.nf index ab2dfbed..a68aa333 100644 --- a/modules/local/annotate_boolean_peaks.nf +++ b/modules/local/annotate_boolean_peaks.nf @@ -2,7 +2,7 @@ process ANNOTATE_BOOLEAN_PEAKS { tag "$meta.id" label 'process_low' - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'ubuntu:20.04' }" diff --git a/modules/local/bam_remove_orphans.nf b/modules/local/bam_remove_orphans.nf index 21ffc73a..95a56c60 100644 --- a/modules/local/bam_remove_orphans.nf +++ b/modules/local/bam_remove_orphans.nf @@ -5,7 +5,7 @@ process BAM_REMOVE_ORPHANS { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::pysam=0.19.0 bioconda::samtools=1.15.1" : null) + conda "bioconda::pysam=0.19.0 bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-57736af1eb98c01010848572c9fec9fff6ffaafd:402e865b8f6af2f3e58c6fc8d57127ff0144b2c7-0' : 'quay.io/biocontainers/mulled-v2-57736af1eb98c01010848572c9fec9fff6ffaafd:402e865b8f6af2f3e58c6fc8d57127ff0144b2c7-0' }" diff --git a/modules/local/bamtools_filter.nf b/modules/local/bamtools_filter.nf index ee469ffd..c1e750d7 100644 --- a/modules/local/bamtools_filter.nf +++ b/modules/local/bamtools_filter.nf @@ -2,7 +2,7 @@ process BAMTOOLS_FILTER { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::bamtools=2.5.2 bioconda::samtools=1.15.1" : null) + conda "bioconda::bamtools=2.5.2 bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' : 'quay.io/biocontainers/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' }" diff --git a/modules/local/bedtools_genomecov.nf b/modules/local/bedtools_genomecov.nf index e8cbb3f7..2f38c06c 100644 --- a/modules/local/bedtools_genomecov.nf +++ b/modules/local/bedtools_genomecov.nf @@ -2,7 +2,7 @@ process BEDTOOLS_GENOMECOV { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::bedtools=2.30.0" : null) + conda "bioconda::bedtools=2.30.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0': 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" diff --git a/modules/local/deseq2_qc.nf b/modules/local/deseq2_qc.nf index 84ff0d5f..fe389300 100644 --- a/modules/local/deseq2_qc.nf +++ b/modules/local/deseq2_qc.nf @@ -4,7 +4,7 @@ process DESEQ2_QC { // (Bio)conda packages have intentionally not been pinned to a specific version // This was to avoid the pipeline failing due to package conflicts whilst creating the environment when using -profile conda - conda (params.enable_conda ? "conda-forge::r-base bioconda::bioconductor-deseq2 bioconda::bioconductor-biocparallel bioconda::bioconductor-tximport bioconda::bioconductor-complexheatmap conda-forge::r-optparse conda-forge::r-ggplot2 conda-forge::r-rcolorbrewer conda-forge::r-pheatmap" : null) + conda "conda-forge::r-base bioconda::bioconductor-deseq2 bioconda::bioconductor-biocparallel bioconda::bioconductor-tximport bioconda::bioconductor-complexheatmap conda-forge::r-optparse conda-forge::r-ggplot2 conda-forge::r-rcolorbrewer conda-forge::r-pheatmap" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' : 'quay.io/biocontainers/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' }" diff --git a/modules/local/frip_score.nf b/modules/local/frip_score.nf index 337b18c5..ffcc6e2d 100644 --- a/modules/local/frip_score.nf +++ b/modules/local/frip_score.nf @@ -2,7 +2,7 @@ process FRIP_SCORE { tag "$meta.id" label 'process_medium' - conda (params.enable_conda ? "bioconda::bedtools=2.30.0 bioconda::samtools=1.15.1" : null) + conda "bioconda::bedtools=2.30.0 bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-8186960447c5cb2faa697666dc1e6d919ad23f3e:3127fcae6b6bdaf8181e21a26ae61231030a9fcb-0': 'quay.io/biocontainers/mulled-v2-8186960447c5cb2faa697666dc1e6d919ad23f3e:3127fcae6b6bdaf8181e21a26ae61231030a9fcb-0' }" diff --git a/modules/local/genome_blacklist_regions.nf b/modules/local/genome_blacklist_regions.nf index 1a28af0f..4421fb6c 100644 --- a/modules/local/genome_blacklist_regions.nf +++ b/modules/local/genome_blacklist_regions.nf @@ -4,7 +4,7 @@ process GENOME_BLACKLIST_REGIONS { tag "$sizes" - conda (params.enable_conda ? "bioconda::bedtools=2.30.0" : null) + conda "bioconda::bedtools=2.30.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0': 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf index 1d306cee..91e29062 100644 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -2,7 +2,7 @@ process GTF2BED { tag "$gtf" label 'process_low' - conda (params.enable_conda ? "conda-forge::perl=5.26.2" : null) + conda "conda-forge::perl=5.26.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/perl:5.26.2': 'quay.io/biocontainers/perl:5.26.2' }" diff --git a/modules/local/igv.nf b/modules/local/igv.nf index 213904b3..59a2d2e8 100644 --- a/modules/local/igv.nf +++ b/modules/local/igv.nf @@ -3,7 +3,7 @@ */ process IGV { - conda (params.enable_conda ? "conda-forge::python=3.8.3" : null) + conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3': 'quay.io/biocontainers/python:3.8.3' }" diff --git a/modules/local/macs2_consensus.nf b/modules/local/macs2_consensus.nf index ab9ef93a..b8a5abd7 100644 --- a/modules/local/macs2_consensus.nf +++ b/modules/local/macs2_consensus.nf @@ -5,7 +5,7 @@ process MACS2_CONSENSUS { tag "$meta.id" label 'process_long' - conda (params.enable_conda ? "conda-forge::biopython conda-forge::r-optparse=1.7.1 conda-forge::r-upsetr=1.4.0 bioconda::bedtools=2.30.0" : null) + conda "conda-forge::biopython conda-forge::r-optparse=1.7.1 conda-forge::r-upsetr=1.4.0 bioconda::bedtools=2.30.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-2f48cc59b03027e31ead6d383fe1b8057785dd24:5d182f583f4696f4c4d9f3be93052811b383341f-0': 'quay.io/biocontainers/mulled-v2-2f48cc59b03027e31ead6d383fe1b8057785dd24:5d182f583f4696f4c4d9f3be93052811b383341f-0' }" diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index 27dd8f29..31552551 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -1,7 +1,7 @@ process MULTIQC { label 'process_medium' - conda (params.enable_conda ? "bioconda::multiqc=1.13a" : null) + conda "bioconda::multiqc=1.13a" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.13a--pyhdfd78af_1': 'quay.io/biocontainers/multiqc:1.13a--pyhdfd78af_1' }" diff --git a/modules/local/multiqc_custom_peaks.nf b/modules/local/multiqc_custom_peaks.nf index ebef7b13..71dc5abb 100644 --- a/modules/local/multiqc_custom_peaks.nf +++ b/modules/local/multiqc_custom_peaks.nf @@ -1,6 +1,6 @@ process MULTIQC_CUSTOM_PEAKS { tag "$meta.id" - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'ubuntu:20.04' }" diff --git a/modules/local/multiqc_custom_phantompeakqualtools.nf b/modules/local/multiqc_custom_phantompeakqualtools.nf index 4878e2c2..c7f4127f 100644 --- a/modules/local/multiqc_custom_phantompeakqualtools.nf +++ b/modules/local/multiqc_custom_phantompeakqualtools.nf @@ -1,6 +1,6 @@ process MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS { tag "$meta.id" - conda (params.enable_conda ? "conda-forge::r-base=3.5.1" : null) + conda "conda-forge::r-base=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/r-base:3.5.1': 'quay.io/biocontainers/r-base:3.5.1' }" diff --git a/modules/local/plot_homer_annotatepeaks.nf b/modules/local/plot_homer_annotatepeaks.nf index 3375349a..eed17949 100644 --- a/modules/local/plot_homer_annotatepeaks.nf +++ b/modules/local/plot_homer_annotatepeaks.nf @@ -1,7 +1,7 @@ process PLOT_HOMER_ANNOTATEPEAKS { label 'process_medium' - conda (params.enable_conda ? "conda-forge::r-base=4.0.3 conda-forge::r-reshape2=1.4.4 conda-forge::r-optparse=1.6.6 conda-forge::r-ggplot2=3.3.3 conda-forge::r-scales=1.1.1 conda-forge::r-viridis=0.5.1 conda-forge::r-tidyverse=1.3.0 bioconda::bioconductor-biostrings=2.58.0 bioconda::bioconductor-complexheatmap=2.6.2" : null) + conda "conda-forge::r-base=4.0.3 conda-forge::r-reshape2=1.4.4 conda-forge::r-optparse=1.6.6 conda-forge::r-ggplot2=3.3.3 conda-forge::r-scales=1.1.1 conda-forge::r-viridis=0.5.1 conda-forge::r-tidyverse=1.3.0 bioconda::bioconductor-biostrings=2.58.0 bioconda::bioconductor-complexheatmap=2.6.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0': 'quay.io/biocontainers/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0' }" diff --git a/modules/local/plot_macs2_qc.nf b/modules/local/plot_macs2_qc.nf index bbbf1ce8..92f400d5 100644 --- a/modules/local/plot_macs2_qc.nf +++ b/modules/local/plot_macs2_qc.nf @@ -1,7 +1,7 @@ process PLOT_MACS2_QC { label 'process_medium' - conda (params.enable_conda ? "conda-forge::r-base=4.0.3 conda-forge::r-reshape2=1.4.4 conda-forge::r-optparse=1.6.6 conda-forge::r-ggplot2=3.3.3 conda-forge::r-scales=1.1.1 conda-forge::r-viridis=0.5.1 conda-forge::r-tidyverse=1.3.0 bioconda::bioconductor-biostrings=2.58.0 bioconda::bioconductor-complexheatmap=2.6.2" : null) + conda "conda-forge::r-base=4.0.3 conda-forge::r-reshape2=1.4.4 conda-forge::r-optparse=1.6.6 conda-forge::r-ggplot2=3.3.3 conda-forge::r-scales=1.1.1 conda-forge::r-viridis=0.5.1 conda-forge::r-tidyverse=1.3.0 bioconda::bioconductor-biostrings=2.58.0 bioconda::bioconductor-complexheatmap=2.6.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0': 'quay.io/biocontainers/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0' }" diff --git a/modules/local/star_align.nf b/modules/local/star_align.nf index f1988bea..0f2589ad 100644 --- a/modules/local/star_align.nf +++ b/modules/local/star_align.nf @@ -3,7 +3,7 @@ process STAR_ALIGN { label 'process_high' // Note: 2.7X indices incompatible with AWS iGenomes. - conda (params.enable_conda ? "bioconda::star=2.6.1d" : null) + conda "bioconda::star=2.6.1d" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/star:2.6.1d--0' : 'quay.io/biocontainers/star:2.6.1d--0' }" diff --git a/modules/local/star_genomegenerate.nf b/modules/local/star_genomegenerate.nf index 3cd4ff20..904b0714 100644 --- a/modules/local/star_genomegenerate.nf +++ b/modules/local/star_genomegenerate.nf @@ -3,7 +3,7 @@ process STAR_GENOMEGENERATE { label 'process_high' // Note: 2.7X indices incompatible with AWS iGenomes. - conda (params.enable_conda ? "bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0" : null) + conda "bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0' : 'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0' }" From ddddd16b938eec6aacd6051ce27ecbd3aa8322fb Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 25 Apr 2023 22:16:51 +0200 Subject: [PATCH 062/201] Update untar, install untarfiles and update prepare_genomes --- modules.json | 7 +++- modules/nf-core/untar/main.nf | 29 ++++++++-------- modules/nf-core/untarfiles/main.nf | 52 ++++++++++++++++++++++++++++ modules/nf-core/untarfiles/meta.yml | 41 ++++++++++++++++++++++ subworkflows/local/prepare_genome.nf | 8 ++--- 5 files changed, 117 insertions(+), 20 deletions(-) create mode 100644 modules/nf-core/untarfiles/main.nf create mode 100644 modules/nf-core/untarfiles/meta.yml diff --git a/modules.json b/modules.json index 48c56fc0..3f822ac4 100644 --- a/modules.json +++ b/modules.json @@ -172,7 +172,12 @@ }, "untar": { "branch": "master", - "git_sha": "5e34754d42cd2d5d248ca8673c0a53cdf5624905", + "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7", + "installed_by": ["modules"] + }, + "untarfiles": { + "branch": "master", + "git_sha": "2498e243c8c30c7125661e62fc777917d8e343b9", "installed_by": ["modules"] } } diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf index 71eea7b2..3384847a 100644 --- a/modules/nf-core/untar/main.nf +++ b/modules/nf-core/untar/main.nf @@ -2,7 +2,7 @@ process UNTAR { tag "$archive" label 'process_single' - conda (params.enable_conda ? "conda-forge::sed=4.7" : null) + conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : 'ubuntu:20.04' }" @@ -11,8 +11,8 @@ process UNTAR { tuple val(meta), path(archive) output: - tuple val(meta), path("$untar"), emit: untar - path "versions.yml" , emit: versions + tuple val(meta), path("$prefix"), emit: untar + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -20,31 +20,29 @@ process UNTAR { script: def args = task.ext.args ?: '' def args2 = task.ext.args2 ?: '' - untar = archive.toString() - '.tar.gz' + prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, "")) """ - mkdir output + mkdir $prefix ## Ensures --strip-components only applied when top level of tar contents is a directory - ## If just files or multiple directories, place all in output - if [[ \$(tar -tzf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then + ## If just files or multiple directories, place all in prefix + if [[ \$(tar -taf ${archive} | grep -o -P "^.*?\\/" | uniq | wc -l) -eq 1 ]]; then tar \\ - -C output --strip-components 1 \\ - -xzvf \\ + -C $prefix --strip-components 1 \\ + -xavf \\ $args \\ $archive \\ $args2 else tar \\ - -C output \\ - -xzvf \\ + -C $prefix \\ + -xavf \\ $args \\ $archive \\ $args2 fi - mv output ${untar} - cat <<-END_VERSIONS > versions.yml "${task.process}": untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') @@ -52,9 +50,10 @@ process UNTAR { """ stub: - untar = archive.toString() - '.tar.gz' + prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.toString().replaceFirst(/\.[^\.]+(.gz)?$/, "")) """ - touch $untar + mkdir $prefix + touch ${prefix}/file.txt cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/untarfiles/main.nf b/modules/nf-core/untarfiles/main.nf new file mode 100644 index 00000000..8bca09d0 --- /dev/null +++ b/modules/nf-core/untarfiles/main.nf @@ -0,0 +1,52 @@ +process UNTARFILES { + tag "$archive" + label 'process_single' + + conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : + 'ubuntu:20.04' }" + + input: + tuple val(meta), path(archive) + + output: + tuple val(meta), path("${prefix}/**") , emit: files + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def args2 = task.ext.args2 ?: '' + prefix = task.ext.prefix ?: ( meta.id ? "${meta.id}" : archive.baseName.toString().replaceFirst(/\.tar$/, "")) + + """ + mkdir $prefix + + tar \\ + -C $prefix \\ + -xavf \\ + $args \\ + $archive \\ + $args2 + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ + + stub: + prefix = task.ext.prefix ?: "${meta.id}" + """ + mkdir $prefix + touch ${prefix}/file.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + untar: \$(echo \$(tar --version 2>&1) | sed 's/^.*(GNU tar) //; s/ Copyright.*\$//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/untarfiles/meta.yml b/modules/nf-core/untarfiles/meta.yml new file mode 100644 index 00000000..e89aa3d6 --- /dev/null +++ b/modules/nf-core/untarfiles/meta.yml @@ -0,0 +1,41 @@ +name: untarfiles +description: Extract files. +keywords: + - untar + - uncompress +tools: + - untar: + description: | + Extract tar.gz files. + documentation: https://www.gnu.org/software/tar/manual/ + licence: ["GPL-3.0-or-later"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - archive: + type: file + description: File to be untar + pattern: "*.{tar}.{gz}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - files: + type: list + description: A list containing references to individual archive files + pattern: "*/**" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@joseespinosa" + - "@drpatelh" + - "@matthdsm" + - "@jfy133" + - "@pinin4fjords" diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 3a42267e..dd16e1f5 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -12,9 +12,9 @@ include { include { UNTAR as UNTAR_BWA_INDEX UNTAR as UNTAR_BOWTIE2_INDEX - UNTAR as UNTAR_CHROMAP_INDEX UNTAR as UNTAR_STAR_INDEX } from '../../modules/nf-core/untar/main' +include { UNTARFILES } from '../../modules/nf-core/untarfiles/main' include { GFFREAD } from '../../modules/nf-core/gffread/main' include { CUSTOM_GETCHROMSIZES } from '../../modules/nf-core/custom/getchromsizes/main' include { BWA_INDEX } from '../../modules/nf-core/bwa/index/main' @@ -138,7 +138,7 @@ workflow PREPARE_GENOME { if (prepare_tool_index == 'bwa') { if (params.bwa_index) { if (params.bwa_index.endsWith('.tar.gz')) { - ch_bwa_index = UNTAR_BWA_INDEX ( [ [:], params.bwa_index ] ).untar.map{ it[1] } + ch_bwa_index = UNTAR_BWA_INDEX ( [ [:], params.bwa_index ] ).untar ch_versions = ch_versions.mix(UNTAR_BWA_INDEX.out.versions) } else { ch_bwa_index = file(params.bwa_index) @@ -174,8 +174,8 @@ workflow PREPARE_GENOME { if (prepare_tool_index == 'chromap') { if (params.chromap_index) { if (params.chromap_index.endsWith('.tar.gz')) { - ch_chromap_index = UNTAR_CHROMAP_INDEX ( [ [:], params.chromap_index ] ).untar.map{ it[1] } - ch_versions = ch_versions.mix(UNTAR.out.versions) + ch_chromap_index = UNTARFILES ( [ [:], params.chromap_index ] ).files + ch_versions = ch_versions.mix(UNTARFILES.out.versions) } else { ch_chromap_index = [ [:], file(params.chromap_index) ] } From 2c7b166ba49d29a02cd016f4fa074c82df1b03bd Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 25 Apr 2023 22:17:22 +0200 Subject: [PATCH 063/201] Delete duplicate instance of chromap index --- conf/modules.config | 8 -------- 1 file changed, 8 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 0381c658..06a1e5a6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -258,14 +258,6 @@ if (params.aligner == 'bowtie2') { if (params.aligner == 'chromap') { process { - withName: CHROMAP_INDEX { - ext.args = '' - publishDir = [ - path: { "${params.outdir}/genome/${params.aligner}/index" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } withName: CHROMAP_CHROMAP { ext.args = '-l 2000 --low-mem --SAM' ext.prefix = { "${meta.id}.Lb" } From 9a7ab508bf65fb1607f63bf3dfb62f38f5c0ca45 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 25 Apr 2023 22:17:30 +0200 Subject: [PATCH 064/201] Fix typo in igenomes --- conf/igenomes.config | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/conf/igenomes.config b/conf/igenomes.config index 7f282cee..2908cad1 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -655,11 +655,11 @@ params { readme = "${params.igenomes_base}/Saccharomyces_cerevisiae/UCSC/sacCer3/Annotation/README.txt" mito_name = "chrM" macs_gsize = [ - "50" : "11624332", - "75" : "11693438", - "100" : "11777680", - "150" : "11783749", - "200" : "11825681" + "50" : 11624332, + "75" : 11693438, + "100" : 11777680, + "150" : 11783749, + "200" : 11825681 ] } 'susScr3' { From fce1f5422c6fddbe341cb7ebdba9778ffbe19770 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 25 Apr 2023 22:40:14 +0200 Subject: [PATCH 065/201] Update changelog --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index a2ed9aaa..9c19fed9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -15,6 +15,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#318](https://github.com/nf-core/chipseq/issues/318)] Update `bowtie2/align` module to fix issue when downloading its singularity image. - [[#320](https://github.com/nf-core/chipseq/issues/320)] Fix samplesheet control column in documentation examples. - [[#328](https://github.com/nf-core/chipseq/issues/328)] Modify documentation to clarify that is necessary to provide the `--read_length` when `--genome` is set and `--macs_gsize` has not provided. +- Remove `enable_conda` param from local modules. +- Fix the path where `chromap` index is stored when `--save_reference` is set. +- Fix untar of `chromap` index when using `--chromap_index` param. ### Software dependencies From a7374e81bc1a4740b184a855d0aa6be14b7d7591 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Apr 2023 12:14:44 +0200 Subject: [PATCH 066/201] Add meta2 to idx in star_align local module to unify prepare_genome uptake of indexes --- modules/local/star_align.nf | 4 ++-- subworkflows/local/prepare_genome.nf | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/local/star_align.nf b/modules/local/star_align.nf index 0f2589ad..be244042 100644 --- a/modules/local/star_align.nf +++ b/modules/local/star_align.nf @@ -9,8 +9,8 @@ process STAR_ALIGN { 'quay.io/biocontainers/star:2.6.1d--0' }" input: - tuple val(meta), path(reads) - path index + tuple val(meta) , path(reads) + tuple val(meta2), path(index) val seq_center output: diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index dd16e1f5..becb9b85 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -192,7 +192,7 @@ workflow PREPARE_GENOME { if (prepare_tool_index == 'star') { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { - ch_star_index = UNTAR_STAR_INDEX ( [ [:], params.star_index ] ).untar.map{ it[1] } + ch_star_index = UNTAR_STAR_INDEX ( [ [:], params.star_index ] ).untar ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { ch_star_index = file(params.star_index) From b6a551718bf80535684efa3b130e282544c99eb2 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Apr 2023 13:26:32 +0200 Subject: [PATCH 067/201] Revert previous commit --- modules/local/star_align.nf | 2 +- subworkflows/local/prepare_genome.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/star_align.nf b/modules/local/star_align.nf index be244042..ec7fc2af 100644 --- a/modules/local/star_align.nf +++ b/modules/local/star_align.nf @@ -10,7 +10,7 @@ process STAR_ALIGN { input: tuple val(meta) , path(reads) - tuple val(meta2), path(index) + path index val seq_center output: diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index becb9b85..dd16e1f5 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -192,7 +192,7 @@ workflow PREPARE_GENOME { if (prepare_tool_index == 'star') { if (params.star_index) { if (params.star_index.endsWith('.tar.gz')) { - ch_star_index = UNTAR_STAR_INDEX ( [ [:], params.star_index ] ).untar + ch_star_index = UNTAR_STAR_INDEX ( [ [:], params.star_index ] ).untar.map{ it[1] } ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { ch_star_index = file(params.star_index) From 9b4a92a885010e56778a1b52eaf2e4fbc69cc53f Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 28 Apr 2023 14:19:16 +0000 Subject: [PATCH 068/201] Template update for nf-core/tools version 2.8 --- .editorconfig | 2 +- .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- .github/PULL_REQUEST_TEMPLATE.md | 3 +- .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 24 ++++ .github/workflows/linting.yml | 2 +- .pre-commit-config.yaml | 5 + README.md | 74 ++++++---- bin/check_samplesheet.py | 3 - conf/base.config | 2 +- conf/igenomes.config | 8 ++ conf/test_full.config | 2 + docs/usage.md | 130 +++++------------- lib/NfcoreSchema.groovy | 4 +- lib/WorkflowChipseq.groovy | 12 +- lib/WorkflowMain.groovy | 13 +- main.nf | 1 - modules.json | 4 +- modules/local/samplesheet_check.nf | 2 +- .../custom/dumpsoftwareversions/main.nf | 6 +- .../custom/dumpsoftwareversions/meta.yml | 2 + modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 3 +- nextflow.config | 29 +++- tower.yml | 5 + 27 files changed, 193 insertions(+), 157 deletions(-) create mode 100644 .github/workflows/clean-up.yml create mode 100644 .pre-commit-config.yaml create mode 100644 tower.yml diff --git a/.editorconfig b/.editorconfig index b78de6e6..b6b31907 100644 --- a/.editorconfig +++ b/.editorconfig @@ -8,7 +8,7 @@ trim_trailing_whitespace = true indent_size = 4 indent_style = space -[*.{md,yml,yaml,html,css,scss,js,cff}] +[*.{md,yml,yaml,html,css,scss,js}] indent_size = 2 # These files are edited and tested upstream in nf-core/modules diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index 99d3b9bf..d71ef79d 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -45,6 +45,6 @@ body: * Nextflow version _(eg. 22.10.1)_ * Hardware _(eg. HPC, Desktop, Cloud)_ * Executor _(eg. slurm, local, awsbatch)_ - * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter or Charliecloud)_ + * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_ * OS _(eg. CentOS Linux, macOS, Linux Mint)_ * Version of nf-core/chipseq _(eg. 1.1, 1.5, 1.8.2)_ diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 8da27af0..f47ffb89 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -15,7 +15,8 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/chip - [ ] This comment contains a description of changes (with reason). - [ ] If you've fixed a bug or added code that should be tested, add tests! -- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/chipseq/tree/master/.github/CONTRIBUTING.md)- [ ] If necessary, also make a PR on the nf-core/chipseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. +- [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/chipseq/tree/master/.github/CONTRIBUTING.md) +- [ ] If necessary, also make a PR on the nf-core/chipseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index d54a33a5..31ac32b9 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: nf-core/tower-action@v3 + uses: seqeralabs/action-tower-launch@v1 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index ecd16998..153490d0 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: nf-core/tower-action@v3 + uses: seqeralabs/action-tower-launch@v1 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 1e03d409..cc0e5a11 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -13,7 +13,7 @@ jobs: - name: Check PRs if: github.repository == 'nf-core/chipseq' run: | - { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/chipseq ]] && [[ $GITHUB_HEAD_REF = "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] + { [[ ${{github.event.pull_request.head.repo.full_name }} == nf-core/chipseq ]] && [[ $GITHUB_HEAD_REF == "dev" ]]; } || [[ $GITHUB_HEAD_REF == "patch" ]] # If the above check failed, post a comment on the PR explaining the failure # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml new file mode 100644 index 00000000..694e90ec --- /dev/null +++ b/.github/workflows/clean-up.yml @@ -0,0 +1,24 @@ +name: "Close user-tagged issues and PRs" +on: + schedule: + - cron: "0 0 * * 0" # Once a week + +jobs: + clean-up: + runs-on: ubuntu-latest + permissions: + issues: write + pull-requests: write + steps: + - uses: actions/stale@v7 + with: + stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." + stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." + close-issue-message: "This issue was closed because it has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor and then staled for 20 days with no activity." + days-before-stale: 30 + days-before-close: 20 + days-before-pr-close: -1 + any-of-labels: "awaiting-changes,awaiting-feedback" + exempt-issue-labels: "WIP" + exempt-pr-labels: "WIP" + repo-token: "${{ secrets.GITHUB_TOKEN }}" diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 858d622e..888cb4bc 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -78,7 +78,7 @@ jobs: - uses: actions/setup-python@v4 with: - python-version: "3.7" + python-version: "3.8" architecture: "x64" - name: Install dependencies diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml new file mode 100644 index 00000000..0c31cdb9 --- /dev/null +++ b/.pre-commit-config.yaml @@ -0,0 +1,5 @@ +repos: + - repo: https://github.com/pre-commit/mirrors-prettier + rev: "v2.7.1" + hooks: + - id: prettier diff --git a/README.md b/README.md index eae41b5f..9a7611f6 100644 --- a/README.md +++ b/README.md @@ -8,57 +8,71 @@ [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) [![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/chipseq) -[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23chipseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/chipseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) +[![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23chipseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/chipseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) ## Introduction - +**nf-core/chipseq** is a bioinformatics pipeline that ... -**nf-core/chipseq** is a bioinformatics best-practice analysis pipeline for ChIP-seq peak-calling and differential analysis pipeline.. - -The pipeline is built using [Nextflow](https://www.nextflow.io), a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from [nf-core/modules](https://github.com/nf-core/modules) in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! - - - -On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources.The results obtained from the full-sized test can be viewed on the [nf-core website](https://nf-co.re/chipseq/results). - -## Pipeline summary + + 1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)) 2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/)) -## Quick Start +## Usage + +> **Note** +> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how +> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) +> with `-profile test` before running the workflow on actual data. + + - Note that some form of configuration will be needed so that Nextflow knows how to fetch the required software. This is usually done in the form of a config profile (`YOURPROFILE` in the example command above). You can chain multiple config profiles in a comma-separated string. +Now, you can run the pipeline using: - > - The pipeline comes with config profiles called `docker`, `singularity`, `podman`, `shifter`, `charliecloud` and `conda` which instruct the pipeline to use the named tool for software management. For example, `-profile test,docker`. - > - Please check [nf-core/configs](https://github.com/nf-core/configs#documentation) to see if a custom config file to run nf-core pipelines already exists for your Institute. If so, you can simply use `-profile ` in your command. This will enable either `docker` or `singularity` and set the appropriate execution settings for your local compute environment. - > - If you are using `singularity`, please use the [`nf-core download`](https://nf-co.re/tools/#downloading-pipelines-for-offline-use) command to download images first, before running the pipeline. Setting the [`NXF_SINGULARITY_CACHEDIR` or `singularity.cacheDir`](https://www.nextflow.io/docs/latest/singularity.html?#singularity-docker-hub) Nextflow options enables you to store and re-use the images from a central location for future pipeline runs. - > - If you are using `conda`, it is highly recommended to use the [`NXF_CONDA_CACHEDIR` or `conda.cacheDir`](https://www.nextflow.io/docs/latest/conda.html) settings to store the environments in a central location for future pipeline runs. + -4. Start running your own analysis! +```bash +nextflow run nf-core/chipseq \ + -profile \ + --input samplesheet.csv \ + --outdir +``` - +> **Warning:** +> Please provide pipeline parameters via the CLI or Nextflow `-params-file` option. Custom config files including those +> provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; +> see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). - ```bash - nextflow run nf-core/chipseq --input samplesheet.csv --outdir --genome GRCh37 -profile - ``` +For more details, please refer to the [usage documentation](https://nf-co.re/chipseq/usage) and the [parameter documentation](https://nf-co.re/chipseq/parameters). -## Documentation +## Pipeline output -The nf-core/chipseq pipeline comes with documentation about the pipeline [usage](https://nf-co.re/chipseq/usage), [parameters](https://nf-co.re/chipseq/parameters) and [output](https://nf-co.re/chipseq/output). +To see the the results of a test run with a full size dataset refer to the [results](https://nf-co.re/chipseq/results) tab on the nf-core website pipeline page. +For more details about the output files and reports, please refer to the +[output documentation](https://nf-co.re/chipseq/output). ## Credits diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 11b15572..4a758fe0 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -158,9 +158,6 @@ def sniff_format(handle): peek = read_head(handle) handle.seek(0) sniffer = csv.Sniffer() - if not sniffer.has_header(peek): - logger.critical("The given sample sheet does not appear to contain a header.") - sys.exit(1) dialect = sniffer.sniff(peek) return dialect diff --git a/conf/base.config b/conf/base.config index daf12c7c..6ff52bc0 100644 --- a/conf/base.config +++ b/conf/base.config @@ -15,7 +15,7 @@ process { memory = { check_max( 6.GB * task.attempt, 'memory' ) } time = { check_max( 4.h * task.attempt, 'time' ) } - errorStrategy = { task.exitStatus in [143,137,104,134,139] ? 'retry' : 'finish' } + errorStrategy = { task.exitStatus in ((130..145) + 104) ? 'retry' : 'finish' } maxRetries = 1 maxErrors = '-1' diff --git a/conf/igenomes.config b/conf/igenomes.config index 7a1b3ac6..3f114377 100644 --- a/conf/igenomes.config +++ b/conf/igenomes.config @@ -36,6 +36,14 @@ params { macs_gsize = "2.7e9" blacklist = "${projectDir}/assets/blacklists/hg38-blacklist.bed" } + 'CHM13' { + fasta = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/WholeGenomeFasta/genome.fa" + bwa = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAIndex/" + bwamem2 = "${params.igenomes_base}/Homo_sapiens/UCSC/CHM13/Sequence/BWAmem2Index/" + gtf = "${params.igenomes_base}/Homo_sapiens/NCBI/CHM13/Annotation/Genes/genes.gtf" + gff = "ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF/009/914/755/GCF_009914755.1_T2T-CHM13v2.0/GCF_009914755.1_T2T-CHM13v2.0_genomic.gff.gz" + mito_name = "chrM" + } 'GRCm38' { fasta = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/WholeGenomeFasta/genome.fa" bwa = "${params.igenomes_base}/Mus_musculus/Ensembl/GRCm38/Sequence/BWAIndex/version0.6.0/" diff --git a/conf/test_full.config b/conf/test_full.config index 5c3bc080..1505bc42 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -10,6 +10,8 @@ ---------------------------------------------------------------------------------------- */ +cleanup = true + params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' diff --git a/docs/usage.md b/docs/usage.md index 6ed6fb45..9f415ef9 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -71,6 +71,29 @@ work # Directory containing the nextflow working files # Other nextflow hidden files, eg. history of pipeline runs and old logs. ``` +If you wish to repeatedly use the same parameters for multiple runs, rather than specifying each flag in the command, you can specify these in a params file. + +Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. + +> ⚠️ Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). +> The above pipeline run specified with a params file in yaml format: + +```bash +nextflow run nf-core/chipseq -profile docker -params-file params.yaml +``` + +with `params.yaml` containing: + +```yaml +input: './samplesheet.csv' +outdir: './results/' +genome: 'GRCh37' +input: 'data' +<...> +``` + +You can also generate such `YAML`/`JSON` files via [nf-core/launch](https://nf-co.re/launch). + ### Updating the pipeline When you run the above command, Nextflow automatically pulls the pipeline code from GitHub and stores it as a cached version. When running the pipeline after this, it will always use the cached version if available - even if the pipeline has been updated since. To make sure that you're running the latest version of the pipeline, make sure that you regularly update the cached version of the pipeline: @@ -87,6 +110,10 @@ First, go to the [nf-core/chipseq releases page](https://github.com/nf-core/chip This version number will be logged in reports when you run the pipeline, so that you'll know what you used when you look back in the future. For example, at the bottom of the MultiQC reports. +To further assist in reproducbility, you can use share and re-use [parameter files](#running-the-pipeline) to repeat pipeline runs with the same settings without having to write out a command with every single parameter. + +> 💡 If you wish to share such profile (such as upload as supplementary material for academic publications), make sure to NOT include cluster specific paths to files, nor institutional specific profiles. + ## Core Nextflow arguments > **NB:** These options are part of Nextflow and use a _single_ hyphen (pipeline parameters use a double-hyphen). @@ -95,7 +122,7 @@ This version number will be logged in reports when you run the pipeline, so that Use this parameter to choose a configuration profile. Profiles can give configuration presets for different compute environments. -Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Conda) - see below. +Several generic profiles are bundled with the pipeline which instruct the pipeline to use software packaged using different methods (Docker, Singularity, Podman, Shifter, Charliecloud, Apptainer, Conda) - see below. > We highly recommend the use of Docker or Singularity containers for full pipeline reproducibility, however when this is not possible, Conda is also supported. @@ -119,8 +146,10 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Shifter](https://nersc.gitlab.io/development/shifter/how-to-use/) - `charliecloud` - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) +- `apptainer` + - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) - `conda` - - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter or Charliecloud. + - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. ### `-resume` @@ -138,102 +167,19 @@ Specify the path to a specific config file (this is a core Nextflow command). Se Whilst the default requirements set within the pipeline will hopefully work for most people and with most input data, you may find that you want to customise the compute resources that the pipeline requests. Each step in the pipeline has a default set of requirements for number of CPUs, memory and time. For most of the steps in the pipeline, if the job exits with any of the error codes specified [here](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L18) it will automatically be resubmitted with higher requests (2 x original, then 3 x original). If it still fails after the third attempt then the pipeline execution is stopped. -For example, if the nf-core/rnaseq pipeline is failing after multiple re-submissions of the `STAR_ALIGN` process due to an exit code of `137` this would indicate that there is an out of memory issue: - -```console -[62/149eb0] NOTE: Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) -- Execution is retried (1) -Error executing process > 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)' - -Caused by: - Process `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN (WT_REP1)` terminated with an error exit status (137) - -Command executed: - STAR \ - --genomeDir star \ - --readFilesIn WT_REP1_trimmed.fq.gz \ - --runThreadN 2 \ - --outFileNamePrefix WT_REP1. \ - - -Command exit status: - 137 - -Command output: - (empty) - -Command error: - .command.sh: line 9: 30 Killed STAR --genomeDir star --readFilesIn WT_REP1_trimmed.fq.gz --runThreadN 2 --outFileNamePrefix WT_REP1. -Work dir: - /home/pipelinetest/work/9d/172ca5881234073e8d76f2a19c88fb - -Tip: you can replicate the issue by changing to the process work dir and entering the command `bash .command.run` -``` - -#### For beginners - -A first step to bypass this error, you could try to increase the amount of CPUs, memory, and time for the whole pipeline. Therefor you can try to increase the resource for the parameters `--max_cpus`, `--max_memory`, and `--max_time`. Based on the error above, you have to increase the amount of memory. Therefore you can go to the [parameter documentation of rnaseq](https://nf-co.re/rnaseq/3.9/parameters) and scroll down to the `show hidden parameter` button to get the default value for `--max_memory`. In this case 128GB, you than can try to run your pipeline again with `--max_memory 200GB -resume` to skip all process, that were already calculated. If you can not increase the resource of the complete pipeline, you can try to adapt the resource for a single process as mentioned below. - -#### Advanced option on process level - -To bypass this error you would need to find exactly which resources are set by the `STAR_ALIGN` process. The quickest way is to search for `process STAR_ALIGN` in the [nf-core/rnaseq Github repo](https://github.com/nf-core/rnaseq/search?q=process+STAR_ALIGN). -We have standardised the structure of Nextflow DSL2 pipelines such that all module files will be present in the `modules/` directory and so, based on the search results, the file we want is `modules/nf-core/star/align/main.nf`. -If you click on the link to that file you will notice that there is a `label` directive at the top of the module that is set to [`label process_high`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/modules/nf-core/software/star/align/main.nf#L9). -The [Nextflow `label`](https://www.nextflow.io/docs/latest/process.html#label) directive allows us to organise workflow processes in separate groups which can be referenced in a configuration file to select and configure subset of processes having similar computing requirements. -The default values for the `process_high` label are set in the pipeline's [`base.config`](https://github.com/nf-core/rnaseq/blob/4c27ef5610c87db00c3c5a3eed10b1d161abf575/conf/base.config#L33-L37) which in this case is defined as 72GB. -Providing you haven't set any other standard nf-core parameters to **cap** the [maximum resources](https://nf-co.re/usage/configuration#max-resources) used by the pipeline then we can try and bypass the `STAR_ALIGN` process failure by creating a custom config file that sets at least 72GB of memory, in this case increased to 100GB. -The custom config below can then be provided to the pipeline via the [`-c`](#-c) parameter as highlighted in previous sections. - -```nextflow -process { - withName: 'NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN' { - memory = 100.GB - } -} -``` - -> **NB:** We specify the full process name i.e. `NFCORE_RNASEQ:RNASEQ:ALIGN_STAR:STAR_ALIGN` in the config file because this takes priority over the short name (`STAR_ALIGN`) and allows existing configuration using the full process name to be correctly overridden. -> -> If you get a warning suggesting that the process selector isn't recognised check that the process name has been specified correctly. - -### Updating containers (advanced users) - -The [Nextflow DSL2](https://www.nextflow.io/docs/latest/dsl2.html) implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. If for some reason you need to use a different version of a particular tool with the pipeline then you just need to identify the `process` name and override the Nextflow `container` definition for that process using the `withName` declaration. For example, in the [nf-core/viralrecon](https://nf-co.re/viralrecon) pipeline a tool called [Pangolin](https://github.com/cov-lineages/pangolin) has been used during the COVID-19 pandemic to assign lineages to SARS-CoV-2 genome sequenced samples. Given that the lineage assignments change quite frequently it doesn't make sense to re-release the nf-core/viralrecon everytime a new version of Pangolin has been released. However, you can override the default container used by the pipeline by creating a custom config file and passing it as a command-line argument via `-c custom.config`. - -1. Check the default version used by the pipeline in the module file for [Pangolin](https://github.com/nf-core/viralrecon/blob/a85d5969f9025409e3618d6c280ef15ce417df65/modules/nf-core/software/pangolin/main.nf#L14-L19) -2. Find the latest version of the Biocontainer available on [Quay.io](https://quay.io/repository/biocontainers/pangolin?tag=latest&tab=tags) -3. Create the custom config accordingly: - - - For Docker: +To change the resource requests, please see the [max resources](https://nf-co.re/docs/usage/configuration#max-resources) and [tuning workflow resources](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources) section of the nf-core website. - ```nextflow - process { - withName: PANGOLIN { - container = 'quay.io/biocontainers/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` +### Custom Containers - - For Singularity: +In some cases you may wish to change which container or conda environment a step of the pipeline uses for a particular tool. By default nf-core pipelines use containers and software from the [biocontainers](https://biocontainers.pro/) or [bioconda](https://bioconda.github.io/) projects. However in some cases the pipeline specified version maybe out of date. - ```nextflow - process { - withName: PANGOLIN { - container = 'https://depot.galaxyproject.org/singularity/pangolin:3.0.5--pyhdfd78af_0' - } - } - ``` +To use a different container from the default container or conda environment specified in a pipeline, please see the [updating tool versions](https://nf-co.re/docs/usage/configuration#updating-tool-versions) section of the nf-core website. - - For Conda: +### Custom Tool Arguments - ```nextflow - process { - withName: PANGOLIN { - conda = 'bioconda::pangolin=3.0.5' - } - } - ``` +A pipeline might not always support every possible argument or option of a particular tool used in pipeline. Fortunately, nf-core pipelines provide some freedom to users to insert additional parameters that the pipeline does not include by default. -> **NB:** If you wish to periodically update individual tool-specific results (e.g. Pangolin) generated by the pipeline then you must ensure to keep the `work/` directory otherwise the `-resume` ability of the pipeline will be compromised and it will restart from scratch. +To learn how to provide additional arguments to a particular tool of the pipeline, please see the [customising tool arguments](https://nf-co.re/docs/usage/configuration#customising-tool-arguments) section of the nf-core website. ### nf-core/configs diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy index 33cd4f6e..9b34804d 100755 --- a/lib/NfcoreSchema.groovy +++ b/lib/NfcoreSchema.groovy @@ -2,6 +2,7 @@ // This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. // +import nextflow.Nextflow import org.everit.json.schema.Schema import org.everit.json.schema.loader.SchemaLoader import org.everit.json.schema.ValidationException @@ -83,6 +84,7 @@ class NfcoreSchema { 'stub-run', 'test', 'w', + 'with-apptainer', 'with-charliecloud', 'with-conda', 'with-dag', @@ -177,7 +179,7 @@ class NfcoreSchema { } if (has_error) { - System.exit(1) + Nextflow.error('Exiting!') } } diff --git a/lib/WorkflowChipseq.groovy b/lib/WorkflowChipseq.groovy index b85fcd58..25175f72 100755 --- a/lib/WorkflowChipseq.groovy +++ b/lib/WorkflowChipseq.groovy @@ -2,6 +2,7 @@ // This file holds several functions specific to the workflow/chipseq.nf in the nf-core/chipseq pipeline // +import nextflow.Nextflow import groovy.text.SimpleTemplateEngine class WorkflowChipseq { @@ -14,8 +15,7 @@ class WorkflowChipseq { if (!params.fasta) { - log.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - System.exit(1) + Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." } } @@ -61,17 +61,19 @@ class WorkflowChipseq { def description_html = engine.createTemplate(methods_text).make(meta) return description_html - }// + } + + // // Exit pipeline if incorrect --genome key provided // private static void genomeExistsError(params, log) { if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - log.error "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + " Currently, the available genome keys are:\n" + " ${params.genomes.keySet().join(", ")}\n" + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - System.exit(1) + Nextflow.error(error_string) } } } diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index bfe9c5ea..6e8af09f 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -2,6 +2,8 @@ // This file holds several functions specific to the main.nf workflow in the nf-core/chipseq pipeline // +import nextflow.Nextflow + class WorkflowMain { // @@ -21,7 +23,7 @@ class WorkflowMain { // // Generate help string // - public static String help(workflow, params, log) { + public static String help(workflow, params) { def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" def help_string = '' help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) @@ -34,7 +36,7 @@ class WorkflowMain { // // Generate parameter summary log string // - public static String paramsSummaryLog(workflow, params, log) { + public static String paramsSummaryLog(workflow, params) { def summary_log = '' summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) @@ -49,7 +51,7 @@ class WorkflowMain { public static void initialise(workflow, params, log) { // Print help to screen if required if (params.help) { - log.info help(workflow, params, log) + log.info help(workflow, params) System.exit(0) } @@ -61,7 +63,7 @@ class WorkflowMain { } // Print parameter summary log to screen - log.info paramsSummaryLog(workflow, params, log) + log.info paramsSummaryLog(workflow, params) // Validate workflow parameters via the JSON schema if (params.validate_params) { @@ -81,8 +83,7 @@ class WorkflowMain { // Check input has been provided if (!params.input) { - log.error "Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'" - System.exit(1) + Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") } } // diff --git a/main.nf b/main.nf index 06d6e411..cf50b118 100644 --- a/main.nf +++ b/main.nf @@ -4,7 +4,6 @@ nf-core/chipseq ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Github : https://github.com/nf-core/chipseq - Website: https://nf-co.re/chipseq Slack : https://nfcore.slack.com/channels/chipseq ---------------------------------------------------------------------------------------- diff --git a/modules.json b/modules.json index 6e4968b6..db078d7b 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "76cc4938c1f6ea5c7d83fed1eeffc146787f9543", "installed_by": ["modules"] }, "fastqc": { @@ -17,7 +17,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", + "git_sha": "f2d63bd5b68925f98f572eed70993d205cc694b7", "installed_by": ["modules"] } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 5d5d6afb..c28658f5 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -5,7 +5,7 @@ process SAMPLESHEET_CHECK { conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'quay.io/biocontainers/python:3.8.3' }" + 'biocontainers/python:3.8.3' }" input: path samplesheet diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 3df21765..800a6099 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.13" + conda "bioconda::multiqc=1.14" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index 60b546a0..c32657de 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,7 +1,9 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: - custom + - dump - version tools: - custom: diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 68f66bea..4b604749 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.13" + conda "bioconda::multiqc=1.14" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.13--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.13--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : + 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index ebc29b27..f93b5ee5 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json name: MultiQC description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: @@ -37,7 +38,7 @@ output: description: MultiQC report file pattern: "multiqc_report.html" - data: - type: dir + type: directory description: MultiQC data dir pattern: "multiqc_data" - plots: diff --git a/nextflow.config b/nextflow.config index 8f2539d1..700f3a20 100644 --- a/nextflow.config +++ b/nextflow.config @@ -78,7 +78,11 @@ try { profiles { - debug { process.beforeScript = 'echo $HOSTNAME' } + debug { + dumpHashes = true + process.beforeScript = 'echo $HOSTNAME' + cleanup = false + } conda { conda.enabled = true docker.enabled = false @@ -86,6 +90,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + apptainer.enabled = false } mamba { conda.enabled = true @@ -95,14 +100,18 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + apptainer.enabled = false } docker { docker.enabled = true + docker.registry = 'quay.io' docker.userEmulation = true + conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false + apptainer.enabled = false } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -110,31 +119,49 @@ profiles { singularity { singularity.enabled = true singularity.autoMounts = true + conda.enabled = false docker.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false + apptainer.enabled = false } podman { podman.enabled = true + podman.registry = 'quay.io' + conda.enabled = false docker.enabled = false singularity.enabled = false shifter.enabled = false charliecloud.enabled = false + apptainer.enabled = false } shifter { shifter.enabled = true + conda.enabled = false docker.enabled = false singularity.enabled = false podman.enabled = false charliecloud.enabled = false + apptainer.enabled = false } charliecloud { charliecloud.enabled = true + conda.enabled = false docker.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false + apptainer.enabled = false + } + apptainer { + apptainer.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false } gitpod { executor.name = 'local' diff --git a/tower.yml b/tower.yml new file mode 100644 index 00000000..787aedfe --- /dev/null +++ b/tower.yml @@ -0,0 +1,5 @@ +reports: + multiqc_report.html: + display: "MultiQC HTML report" + samplesheet.csv: + display: "Auto-created samplesheet with collated metadata and FASTQ paths" From 4f271e1a6b6ed910451d8017c61fd9440617b41a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 4 May 2023 11:35:39 +0200 Subject: [PATCH 069/201] Fix unmerged change --- bin/check_samplesheet.py | 12 ------------ 1 file changed, 12 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 4aee34d3..3fd6ff8a 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -25,7 +25,6 @@ def make_dir(path): raise exception -<<<<<<< HEAD def print_error(error, context="Line", context_str=""): error_str = "ERROR: Please check samplesheet -> {}".format(error) if context != "" and context_str != "": @@ -34,17 +33,6 @@ def print_error(error, context="Line", context_str=""): ) print(error_str) sys.exit(1) -======= - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect ->>>>>>> 9b4a92a885010e56778a1b52eaf2e4fbc69cc53f def check_samplesheet(file_in, file_out): From da50a0c043c4ca17891c4ea62eb09b8ed17f73c8 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 4 May 2023 11:38:18 +0200 Subject: [PATCH 070/201] Make prettier happy --- README.md | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/README.md b/README.md index 858bf926..6b82aad6 100644 --- a/README.md +++ b/README.md @@ -86,9 +86,9 @@ WT_INPUT_REP3,BLA203A31_S21_L003_R1_001.fastq.gz,,, Now, you can run the pipeline using: - ```bash - nextflow run nf-core/chipseq --input samplesheet.csv --outdir --genome GRCh37 -profile - ``` +```bash +nextflow run nf-core/chipseq --input samplesheet.csv --outdir --genome GRCh37 -profile +``` See [usage docs](https://nf-co.re/chipseq/usage) for all of the available options when running the pipeline. From c21ba1d0b5e8a52b6ce5217bc8fe0b3e18cfa8fc Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 4 May 2023 11:47:07 +0200 Subject: [PATCH 071/201] Fix local merge changes deleted by mistake --- tower.yml | 16 ++++++++++++++++ 1 file changed, 16 insertions(+) diff --git a/tower.yml b/tower.yml index 787aedfe..096be2bb 100644 --- a/tower.yml +++ b/tower.yml @@ -1,5 +1,21 @@ reports: multiqc_report.html: display: "MultiQC HTML report" + macs2_peak.plots.pdf: + display: "All samples MACS2 peak QC PDF plots" + macs2_annotatePeaks.plots.pdf: + display: "All samples HOMER annotatePeaks.pl QC PDF plots" + "*.consensus_peaks.plots.pdf": + display: "Consensus peaks DESeq2 QC PDF plots" + "*.consensus_peaks.boolean.intersect.plot.pdf": + display: "Consensus peaks UpSetR intersection PDF plots" + "*.consensus_peaks.boolean.annotatePeaks.txt": + display: "Consensus peaks annotated by HOMER" + "*.plotHeatmap.pdf": + display: "Per-sample deepTools plotHeatmap PDF plots" + "*_peaks.broadPeak": + display: "Per-sample MACS2 broadPeak file" + "*_peaks.narrowPeak": + display: "Per-sample MACS2 narrowPeak file" samplesheet.csv: display: "Auto-created samplesheet with collated metadata and FASTQ paths" From eaee70bfc42e39134b437d8ba2d196a83e727b04 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 4 May 2023 12:05:22 +0200 Subject: [PATCH 072/201] reorder tower.yml --- tower.yml | 2 -- 1 file changed, 2 deletions(-) diff --git a/tower.yml b/tower.yml index 096be2bb..5b1f5f90 100644 --- a/tower.yml +++ b/tower.yml @@ -17,5 +17,3 @@ reports: display: "Per-sample MACS2 broadPeak file" "*_peaks.narrowPeak": display: "Per-sample MACS2 narrowPeak file" - samplesheet.csv: - display: "Auto-created samplesheet with collated metadata and FASTQ paths" From e4588b4b7abcbef72e4f02afc917c48bdde12146 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 5 May 2023 10:56:47 +0200 Subject: [PATCH 073/201] Apply review suggestions --- modules/local/annotate_boolean_peaks.nf | 2 +- modules/local/multiqc_custom_peaks.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/annotate_boolean_peaks.nf b/modules/local/annotate_boolean_peaks.nf index e7399f32..77baf5c4 100644 --- a/modules/local/annotate_boolean_peaks.nf +++ b/modules/local/annotate_boolean_peaks.nf @@ -5,7 +5,7 @@ process ANNOTATE_BOOLEAN_PEAKS { conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'docker.io/ubuntu:20.04' }" + 'docker.io/library/ubuntu:20.04' }" input: tuple val(meta), path(boolean_txt), path(homer_peaks) diff --git a/modules/local/multiqc_custom_peaks.nf b/modules/local/multiqc_custom_peaks.nf index 53f765c5..8fabe949 100644 --- a/modules/local/multiqc_custom_peaks.nf +++ b/modules/local/multiqc_custom_peaks.nf @@ -3,7 +3,7 @@ process MULTIQC_CUSTOM_PEAKS { conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'docker.io/ubuntu:20.04' }" + 'docker.io/library/ubuntu:20.04' }" input: tuple val(meta), path(peak), path(frip) From 133ff475d0ac51fc3fde9e371d661d17c426d142 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 12 May 2023 10:26:17 +0200 Subject: [PATCH 074/201] Set a default registry outside of profile scope --- nextflow.config | 8 ++++++-- 1 file changed, 6 insertions(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 6a8e9edd..330eb353 100644 --- a/nextflow.config +++ b/nextflow.config @@ -151,7 +151,6 @@ profiles { } docker { docker.enabled = true - docker.registry = 'quay.io' docker.userEmulation = true conda.enabled = false singularity.enabled = false @@ -175,7 +174,6 @@ profiles { } podman { podman.enabled = true - podman.registry = 'quay.io' conda.enabled = false docker.enabled = false singularity.enabled = false @@ -240,6 +238,12 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Set default registry for Docker and Podman independent of -profile +// Will not be used unless Docker / Podman are enabled +// Set to your registry if you have a mirror of containers +docker.registry = 'quay.io' +podman.registry = 'quay.io' + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true From 647a3e3c07c0e8cbe75b85ba3761ba92bf435f59 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 12 May 2023 10:33:48 +0200 Subject: [PATCH 075/201] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9c19fed9..ec2d167b 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -18,6 +18,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Remove `enable_conda` param from local modules. - Fix the path where `chromap` index is stored when `--save_reference` is set. - Fix untar of `chromap` index when using `--chromap_index` param. +- [nf-core/tools#2286](https://github.com/nf-core/tools/issues/2286) Set default container registry outside profile scope. ### Software dependencies From 667b17f43d5cad15fccafff86cdb549b54a89832 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 20 Jun 2023 23:22:36 +0200 Subject: [PATCH 076/201] Add explicit replicates and control_replicate column --- bin/check_samplesheet.py | 104 +++++++++++++++++++++++++++++---------- 1 file changed, 78 insertions(+), 26 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 3fd6ff8a..f61cc626 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -38,20 +38,20 @@ def print_error(error, context="Line", context_str=""): def check_samplesheet(file_in, file_out): """ This function checks that the samplesheet follows the following structure: - sample,fastq_1,fastq_2,antibody,control - SPT5_T0_REP1,SRR1822153_1.fastq.gz,SRR1822153_2.fastq.gz,SPT5,SPT5_INPUT_REP1 - SPT5_T0_REP2,SRR1822154_1.fastq.gz,SRR1822154_2.fastq.gz,SPT5,SPT5_INPUT_REP2 - SPT5_INPUT_REP1,SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,, - SPT5_INPUT_REP2,SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,, + sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate + SPT5_T0,SRR1822153_1.fastq.gz,SRR1822153_2.fastq.gz,SPT5,1,SPT5_INPUT,1 + SPT5_T0,SRR1822154_1.fastq.gz,SRR1822154_2.fastq.gz,SPT5,2,SPT5_INPUT,2 + SPT5_INPUT,SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,1,,, + SPT5_INPUT,SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,2,,, For an example see: - https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/samplesheet/v2.0/samplesheet_test.csv + https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/samplesheet/v2.1/samplesheet_test.csv """ sample_mapping_dict = {} with open(file_in, "r", encoding="utf-8-sig") as fin: ## Check header - MIN_COLS = 2 - HEADER = ["sample", "fastq_1", "fastq_2", "antibody", "control"] + MIN_COLS = 3 + HEADER = ["sample", "fastq_1", "fastq_2", "replicate", "antibody", "control","control_replicate"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print(f"ERROR: Please check samplesheet header -> {','.join(header)} != {','.join(HEADER)}") @@ -77,7 +77,7 @@ def check_samplesheet(file_in, file_out): ) ## Check sample name entries - sample, fastq_1, fastq_2, antibody, control = lspl[: len(HEADER)] + sample, fastq_1, fastq_2, replicate, antibody, control, control_replicate = lspl[: len(HEADER)] if sample.find(" ") != -1: print(f"WARNING: Spaces have been replaced by underscores for sample: {sample}") sample = sample.replace(" ", "_") @@ -96,6 +96,11 @@ def check_samplesheet(file_in, file_out): line, ) + ## Check replicate column is integer + if not replicate.isdecimal(): + print_error("Replicate id not an integer!", "Line", line) + sys.exit(1) + ## Check antibody and control columns have valid values if antibody: if antibody.find(" ") != -1: @@ -107,10 +112,15 @@ def check_samplesheet(file_in, file_out): "Line", line, ) + if control: if control.find(" ") != -1: print(f"WARNING: Spaces have been replaced by underscores for control: {control}") control = control.replace(" ", "_") + if not control_replicate.isdecimal(): + print_error("Control replicate id not an integer!", "Line", line) + sys.exit(1) + control = "{}_REP{}".format(control, control_replicate) if not antibody: print_error( "Both antibody and control columns must be specified!", @@ -119,22 +129,27 @@ def check_samplesheet(file_in, file_out): ) ## Auto-detect paired-end/single-end - sample_info = [] ## [single_end, fastq_1, fastq_2, antibody, control] + sample_info = [] ## [single_end, fastq_1, fastq_2, replicate, antibody, control] if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ["0", fastq_1, fastq_2, antibody, control] + sample_info = ["0", fastq_1, fastq_2, replicate, antibody,control] elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ["1", fastq_1, fastq_2, antibody, control] + sample_info = ["1", fastq_1, fastq_2, replicate, antibody, control] else: print_error("Invalid combination of columns provided!", "Line", line) - ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, antibody, control ]]} + ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, replicate, antibody, control ]]} + replicate = int(replicate) + sample_info = sample_info + lspl[len(HEADER) :] if sample not in sample_mapping_dict: - sample_mapping_dict[sample] = [sample_info] + sample_mapping_dict[sample] = {} + if replicate not in sample_mapping_dict[sample]: + sample_mapping_dict[sample][replicate] = [sample_info] else: - if sample_info in sample_mapping_dict[sample]: + if sample_info in sample_mapping_dict[sample][replicate]: print_error("Samplesheet contains duplicate rows!", "Line", line) else: - sample_mapping_dict[sample].append(sample_info) + sample_mapping_dict[sample][replicate].append(sample_info) + ## Write validated samplesheet with appropriate columns if len(sample_mapping_dict) > 0: @@ -148,6 +163,7 @@ def check_samplesheet(file_in, file_out): "single_end", "fastq_1", "fastq_2", + "replicate", "antibody", "control", ] @@ -155,24 +171,60 @@ def check_samplesheet(file_in, file_out): + "\n" ) for sample in sorted(sample_mapping_dict.keys()): - ## Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end - if not all(x[0] == sample_mapping_dict[sample][0][0] for x in sample_mapping_dict[sample]): + ## Check that replicate ids are in format 1.. + uniq_rep_ids = sorted(list(set(sample_mapping_dict[sample].keys()))) + if len(uniq_rep_ids) != max(uniq_rep_ids) or 1 != min(uniq_rep_ids): + print_error( + "Replicate ids must start with 1..!", + "Sample", + "{}, replicate ids: {}".format(sample, ",".join([str(x) for x in uniq_rep_ids])), + ) + sys.exit(1) + + ## Check that multiple replicates are of the same datatype i.e. single-end / paired-end + if not all( + x[0][0] == sample_mapping_dict[sample][1][0][0] for x in sample_mapping_dict[sample].values() + ): print_error( - f"Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end!", + f"Multiple replicates of a sample must be of the same datatype i.e. single-end or paired-end!", "Sample", sample, ) - for idx, val in enumerate(sample_mapping_dict[sample]): - control = val[-1] - if control and control not in sample_mapping_dict.keys(): + for replicate in sorted(sample_mapping_dict[sample].keys()): + ## Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + if not all( + x[0] == sample_mapping_dict[sample][replicate][0][0] + for x in sample_mapping_dict[sample][replicate] + ): print_error( - f"Control identifier has to match does a provided sample identifier!", - "Control", - control, + f"Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end!", + "Sample", + sample, ) - fout.write(",".join([f"{sample}_T{idx+1}"] + val) + "\n") + for idx, val in enumerate(sample_mapping_dict[sample][replicate]): + control = "_REP".join(val[-1].split("_REP")[:-1]) + control_replicate = val[-1].split("_REP")[-1] + if control and ( + control not in sample_mapping_dict.keys() + or int(control_replicate) not in sample_mapping_dict[control].keys() + ): + print_error( + f"Control identifier and replicate has to match a provided sample identifier and replicate!", + "Control", + val[4], + ) + + ## Write to file + for idx in range(len(sample_mapping_dict[sample][replicate])): + fastq_files = sample_mapping_dict[sample][replicate][idx] + sample_id = "{}_REP{}_T{}".format(sample, replicate, idx + 1) + if len(fastq_files) == 1: + fout.write(",".join([sample_id] + fastq_files) + ",\n") + else: + fout.write(",".join([sample_id] + fastq_files) + "\n") + else: print_error(f"No entries to process!", "Samplesheet: {file_in}") From 8f1f57e05ab65064c02ae69a43ebd10d4ce45de1 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 20 Jun 2023 23:22:59 +0200 Subject: [PATCH 077/201] Update samplesheet in assets --- assets/samplesheet_pe.csv | 42 +++++++++++++++++++-------------------- assets/samplesheet_se.csv | 42 +++++++++++++++++++-------------------- 2 files changed, 42 insertions(+), 42 deletions(-) diff --git a/assets/samplesheet_pe.csv b/assets/samplesheet_pe.csv index 3a304f12..17ea2e86 100644 --- a/assets/samplesheet_pe.csv +++ b/assets/samplesheet_pe.csv @@ -1,21 +1,21 @@ -sample,fastq_1,fastq_2,antibody,control -WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,BLA203A1_S27_L006_R2_001.fastq.gz,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,BLA203A25_S16_L001_R2_001.fastq.gz,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,BLA203A25_S16_L002_R2_001.fastq.gz,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,BLA203A49_S40_L001_R2_001.fastq.gz,BCATENIN,WT_INPUT -NAIVE_BCATENIN_IP_REP1,BLA203A7_S60_L001_R1_001.fastq.gz,BLA203A7_S60_L001_R2_001.fastq.gz,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L001_R1_001.fastq.gz,BLA203A43_S34_L001_R2_001.fastq.gz,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L002_R1_001.fastq.gz,BLA203A43_S34_L002_R2_001.fastq.gz,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP3,BLA203A64_S55_L001_R1_001.fastq.gz,BLA203A64_S55_L001_R2_001.fastq.gz,BCATENIN,NAIVE_INPUT -WT_TCF4_IP_REP1,BLA203A3_S29_L006_R1_001.fastq.gz,BLA203A3_S29_L006_R2_001.fastq.gz,TCF4,WT_INPUT -WT_TCF4_IP_REP2,BLA203A27_S18_L001_R1_001.fastq.gz,BLA203A27_S18_L001_R2_001.fastq.gz,TCF4,WT_INPUT -WT_TCF4_IP_REP2,BLA203A51_S42_L001_R1_001.fastq.gz,BLA203A51_S42_L001_R2_001.fastq.gz,TCF4,WT_INPUT -NAIVE_TCF4_IP_REP1,BLA203A9_S62_L001_R1_001.fastq.gz,BLA203A9_S62_L001_R2_001.fastq.gz,TCF4,NAIVE_INPUT -NAIVE_TCF4_IP_REP2,BLA203A45_S36_L001_R1_001.fastq.gz,BLA203A45_S36_L001_R2_001.fastq.gz,TCF4,NAIVE_INPUT -NAIVE_TCF4_IP_REP3,BLA203A66_S57_L001_R1_001.fastq.gz,BLA203A66_S57_L001_R2_001.fastq.gz,TCF4,NAIVE_INPUT -WT_INPUT_REP1,BLA203A6_S32_L006_R1_001.fastq.gz,BLA203A6_S32_L006_R2_001.fastq.gz,, -WT_INPUT_REP2,BLA203A30_S21_L001_R1_001.fastq.gz,BLA203A30_S21_L001_R2_001.fastq.gz,, -WT_INPUT_REP3,BLA203A31_S21_L003_R1_001.fastq.gz,BLA203A31_S21_L003_R2_001.fastq.gz,, -NAIVE_INPUT_REP1,BLA203A12_S3_L001_R1_001.fastq.gz,BLA203A12_S3_L001_R2_001.fastq.gz,, -NAIVE_INPUT_REP2,BLA203A48_S39_L001_R1_001.fastq.gz,BLA203A48_S39_L001_R2_001.fastq.gz,, -NAIVE_INPUT_REP3,BLA203A49_S1_L006_R1_001.fastq.gz,BLA203A49_S1_L006_R2_001.fastq.gz,, +sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate +WT_BCATENIN_IP,BLA203A1_S27_L006_R1_001.fastq.gz,BLA203A1_S27_L006_R2_001.fastq.gz,1,BCATENIN,WT_INPUT,1 +WT_BCATENIN_IP,BLA203A25_S16_L001_R1_001.fastq.gz,BLA203A25_S16_L001_R2_001.fastq.gz,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A25_S16_L002_R1_001.fastq.gz,BLA203A25_S16_L002_R2_001.fastq.gz,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A49_S40_L001_R1_001.fastq.gz,BLA203A49_S40_L001_R2_001.fastq.gz,3,BCATENIN,WT_INPUT,3 +NAIVE_BCATENIN_IP,BLA203A7_S60_L001_R1_001.fastq.gz,BLA203A7_S60_L001_R2_001.fastq.gz,1,BCATENIN,NAIVE_INPUT,1 +NAIVE_BCATENIN_IP,BLA203A43_S34_L001_R1_001.fastq.gz,BLA203A43_S34_L001_R2_001.fastq.gz,2,BCATENIN,NAIVE_INPUT,2 +NAIVE_BCATENIN_IP,BLA203A43_S34_L002_R1_001.fastq.gz,BLA203A43_S34_L002_R2_001.fastq.gz,2,BCATENIN,NAIVE_INPUT,2 +NAIVE_BCATENIN_IP,BLA203A64_S55_L001_R1_001.fastq.gz,BLA203A64_S55_L001_R2_001.fastq.gz,3,BCATENIN,NAIVE_INPUT,3 +WT_TCF4_IP,BLA203A3_S29_L006_R1_001.fastq.gz,BLA203A3_S29_L006_R2_001.fastq.gz,1,TCF4,WT_INPUT,1 +WT_TCF4_IP,BLA203A27_S18_L001_R1_001.fastq.gz,BLA203A27_S18_L001_R2_001.fastq.gz,2,TCF4,WT_INPUT,2 +WT_TCF4_IP,BLA203A51_S42_L001_R1_001.fastq.gz,BLA203A51_S42_L001_R2_001.fastq.gz,2,TCF4,WT_INPUT,2 +NAIVE_TCF4_IP,BLA203A9_S62_L001_R1_001.fastq.gz,BLA203A9_S62_L001_R2_001.fastq.gz,1,TCF4,NAIVE_INPUT,1 +NAIVE_TCF4_IP,BLA203A45_S36_L001_R1_001.fastq.gz,BLA203A45_S36_L001_R2_001.fastq.gz,2,TCF4,NAIVE_INPUT,2 +NAIVE_TCF4_IP,BLA203A66_S57_L001_R1_001.fastq.gz,BLA203A66_S57_L001_R2_001.fastq.gz,3,TCF4,NAIVE_INPUT,3 +WT_INPUT,BLA203A6_S32_L006_R1_001.fastq.gz,BLA203A6_S32_L006_R2_001.fastq.gz,1,,, +WT_INPUT,BLA203A30_S21_L001_R1_001.fastq.gz,BLA203A30_S21_L001_R2_001.fastq.gz,2,,, +WT_INPUT,BLA203A31_S21_L003_R1_001.fastq.gz,BLA203A31_S21_L003_R2_001.fastq.gz,3,,, +NAIVE_INPUT,BLA203A12_S3_L001_R1_001.fastq.gz,BLA203A12_S3_L001_R2_001.fastq.gz,1,,, +NAIVE_INPUT,BLA203A48_S39_L001_R1_001.fastq.gz,BLA203A48_S39_L001_R2_001.fastq.gz,2,,, +NAIVE_INPUT,BLA203A49_S1_L006_R1_001.fastq.gz,BLA203A49_S1_L006_R2_001.fastq.gz,3,,, diff --git a/assets/samplesheet_se.csv b/assets/samplesheet_se.csv index a9581d6e..8a0297d7 100644 --- a/assets/samplesheet_se.csv +++ b/assets/samplesheet_se.csv @@ -1,21 +1,21 @@ -sample,fastq_1,fastq_2,antibody,control -WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,,BCATENIN,WT_INPUT -WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT -NAIVE_BCATENIN_IP_REP1,BLA203A7_S60_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L002_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -NAIVE_BCATENIN_IP_REP3,BLA203A64_S55_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT -WT_TCF4_IP_REP1,BLA203A3_S29_L006_R1_001.fastq.gz,,TCF4,WT_INPUT -WT_TCF4_IP_REP2,BLA203A27_S18_L001_R1_001.fastq.gz,,TCF4,WT_INPUT -WT_TCF4_IP_REP3,BLA203A51_S42_L001_R1_001.fastq.gz,,TCF4,WT_INPUT -NAIVE_TCF4_IP_REP1,BLA203A9_S62_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT -NAIVE_TCF4_IP_REP2,BLA203A45_S36_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT -NAIVE_TCF4_IP_REP3,BLA203A66_S57_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT -WT_INPUT_REP1,BLA203A6_S32_L006_R1_001.fastq.gz,,, -WT_INPUT_REP2,BLA203A30_S21_L001_R1_001.fastq.gz,,, -WT_INPUT_REP3,BLA203A31_S21_L003_R1_001.fastq.gz,,, -NAIVE_INPUT_REP1,BLA203A12_S3_L001_R1_001.fastq.gz,,, -NAIVE_INPUT_REP2,BLA203A48_S39_L001_R1_001.fastq.gz,,, -NAIVE_INPUT_REP3,BLA203A49_S1_L006_R1_001.fastq.gz,,, +sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate +WT_BCATENIN_IP,BLA203A1_S27_L006_R1_001.fastq.gz,,1,BCATENIN,WT_INPUT,1 +WT_BCATENIN_IP,BLA203A25_S16_L001_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A25_S16_L002_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A49_S40_L001_R1_001.fastq.gz,,3,BCATENIN,WT_INPUT,3 +NAIVE_BCATENIN_IP,BLA203A7_S60_L001_R1_001.fastq.gz,,1,BCATENIN,NAIVE_INPUT,1 +NAIVE_BCATENIN_IP,BLA203A43_S34_L001_R1_001.fastq.gz,,2,BCATENIN,NAIVE_INPUT,2 +NAIVE_BCATENIN_IP,BLA203A43_S34_L002_R1_001.fastq.gz,,2,BCATENIN,NAIVE_INPUT,2 +NAIVE_BCATENIN_IP,BLA203A64_S55_L001_R1_001.fastq.gz,,3,BCATENIN,NAIVE_INPUT,3 +WT_TCF4_IP,BLA203A3_S29_L006_R1_001.fastq.gz,,1,TCF4,WT_INPUT,1 +WT_TCF4_IP,BLA203A27_S18_L001_R1_001.fastq.gz,,2,TCF4,WT_INPUT,2 +WT_TCF4_IP,BLA203A51_S42_L001_R1_001.fastq.gz,,3,TCF4,WT_INPUT,3 +NAIVE_TCF4_IP,BLA203A9_S62_L001_R1_001.fastq.gz,,1,TCF4,NAIVE_INPUT,1 +NAIVE_TCF4_IP,BLA203A45_S36_L001_R1_001.fastq.gz,,2,TCF4,NAIVE_INPUT,2 +NAIVE_TCF4_IP,BLA203A66_S57_L001_R1_001.fastq.gz,,3,TCF4,NAIVE_INPUT,3 +WT_INPUT,BLA203A6_S32_L006_R1_001.fastq.gz,,1,,, +WT_INPUT,BLA203A30_S21_L001_R1_001.fastq.gz,,2,,, +WT_INPUT,BLA203A31_S21_L003_R1_001.fastq.gz,,3,,, +NAIVE_INPUT,BLA203A12_S3_L001_R1_001.fastq.gz,,1,,, +NAIVE_INPUT,BLA203A48_S39_L001_R1_001.fastq.gz,,2,,, +NAIVE_INPUT,BLA203A49_S1_L006_R1_001.fastq.gz,,3,,, From 14e21f7447ed3752981f053e1075a3a10c9cde4a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 20 Jun 2023 23:23:45 +0200 Subject: [PATCH 078/201] Update code to deal with explicit replicates and control replicates --- conf/test.config | 2 +- workflows/chipseq.nf | 23 +++++++++++++++++------ 2 files changed, 18 insertions(+), 7 deletions(-) diff --git a/conf/test.config b/conf/test.config index 9b24bc9a..f38e12c5 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,7 +20,7 @@ params { max_time = '6.h' // Input data - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/samplesheet/v2.0/samplesheet_test.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/samplesheet/v2.1/samplesheet_test.csv' read_length = 50 // Genome references diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index bdf5783f..1390e114 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -423,11 +423,19 @@ workflow CHIPSEQ { .set { ch_genome_bam_bai } ch_genome_bam_bai - .combine(ch_genome_bam_bai) .map { - meta1, bam1, bai1, meta2, bam2, bai2 -> - meta1.control == meta2.id ? [ meta1, [ bam1, bam2 ], [ bai1, bai2 ] ] : null + meta, bam, bai -> + meta.control ? null : [ meta.id, [ bam ] , [ bai ] ] } + .set { ch_control_bam_bai } + + ch_genome_bam_bai + .map { + meta, bam, bai -> + meta.control ? [ meta.control, meta, [ bam ], [ bai ] ] : null + } + .combine(ch_control_bam_bai, by: 0) + .map { it -> [ it[1] , it[2] + it[4], it[3] + it[5] ] } .set { ch_ip_control_bam_bai } // @@ -482,7 +490,10 @@ workflow CHIPSEQ { MACS2_CALLPEAK .out .peak - .filter { meta, peaks -> peaks.size() > 0 } + .filter { + meta, peaks -> + peaks.size() > 0 + } .set { ch_macs2_peaks } // Create channels: [ meta, ip_bam, peaks ] @@ -564,7 +575,7 @@ workflow CHIPSEQ { ch_deseq2_clustering_multiqc = Channel.empty() if (!params.skip_consensus_peaks) { // Create channels: [ meta , [ peaks ] ] - // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] + // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] ch_macs2_peaks .map { meta, peak -> @@ -588,7 +599,7 @@ workflow CHIPSEQ { [ meta_new, peaks ] } .set { ch_antibody_peaks } - + ch_antibody_peaks.dump() // // MODULE: Generate consensus peaks across samples // From 07ce1527bf5f5c7937828b0033a10b652111477d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 20 Jun 2023 23:37:16 +0200 Subject: [PATCH 079/201] Make black happy --- bin/check_samplesheet.py | 21 ++++++++++----------- 1 file changed, 10 insertions(+), 11 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index f61cc626..b7b285ab 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -51,7 +51,7 @@ def check_samplesheet(file_in, file_out): with open(file_in, "r", encoding="utf-8-sig") as fin: ## Check header MIN_COLS = 3 - HEADER = ["sample", "fastq_1", "fastq_2", "replicate", "antibody", "control","control_replicate"] + HEADER = ["sample", "fastq_1", "fastq_2", "replicate", "antibody", "control", "control_replicate"] header = [x.strip('"') for x in fin.readline().strip().split(",")] if header[: len(HEADER)] != HEADER: print(f"ERROR: Please check samplesheet header -> {','.join(header)} != {','.join(HEADER)}") @@ -131,7 +131,7 @@ def check_samplesheet(file_in, file_out): ## Auto-detect paired-end/single-end sample_info = [] ## [single_end, fastq_1, fastq_2, replicate, antibody, control] if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ["0", fastq_1, fastq_2, replicate, antibody,control] + sample_info = ["0", fastq_1, fastq_2, replicate, antibody, control] elif sample and fastq_1 and not fastq_2: ## Single-end short reads sample_info = ["1", fastq_1, fastq_2, replicate, antibody, control] else: @@ -141,7 +141,7 @@ def check_samplesheet(file_in, file_out): replicate = int(replicate) sample_info = sample_info + lspl[len(HEADER) :] if sample not in sample_mapping_dict: - sample_mapping_dict[sample] = {} + sample_mapping_dict[sample] = {} if replicate not in sample_mapping_dict[sample]: sample_mapping_dict[sample][replicate] = [sample_info] else: @@ -150,7 +150,6 @@ def check_samplesheet(file_in, file_out): else: sample_mapping_dict[sample][replicate].append(sample_info) - ## Write validated samplesheet with appropriate columns if len(sample_mapping_dict) > 0: out_dir = os.path.dirname(file_out) @@ -208,13 +207,13 @@ def check_samplesheet(file_in, file_out): control_replicate = val[-1].split("_REP")[-1] if control and ( control not in sample_mapping_dict.keys() - or int(control_replicate) not in sample_mapping_dict[control].keys() - ): - print_error( - f"Control identifier and replicate has to match a provided sample identifier and replicate!", - "Control", - val[4], - ) + or int(control_replicate) not in sample_mapping_dict[control].keys() + ): + print_error( + f"Control identifier and replicate has to match a provided sample identifier and replicate!", + "Control", + val[4], + ) ## Write to file for idx in range(len(sample_mapping_dict[sample][replicate])): From bb9515de161735a6011440dc820a6313a51e4197 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 21 Jun 2023 09:48:55 +0200 Subject: [PATCH 080/201] Update changelog --- CHANGELOG.md | 15 ++++++++------- 1 file changed, 8 insertions(+), 7 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index ec2d167b..2c9b7821 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,17 +8,18 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ### Enhancements & fixes - Updated pipeline template to [nf-core/tools 2.7.2](https://github.com/nf-core/tools/releases/tag/2.7.2) -- [[#317](https://github.com/nf-core/chipseq/issues/317)] Added metro map -- [[#288](https://github.com/nf-core/chipseq/issues/291)] Bump `chromap` version 2 and enable all the steps below chromap again when paired-end data is processed. -- [[#311](https://github.com/nf-core/chipseq/issues/311)] Add back `--skip_spp` parameter which was unintentionally removed from the code. +- [[#317](https://github.com/nf-core/chipseq/issues/317)] - Added metro map +- [[#288](https://github.com/nf-core/chipseq/issues/291)] - Bump `chromap` version 2 and enable all the steps below chromap again when paired-end data is processed. +- [[#311](https://github.com/nf-core/chipseq/issues/311)] - Add back `--skip_spp` parameter which was unintentionally removed from the code. - Install available nf-core subworkflows and refactor code accordingly -- [[#318](https://github.com/nf-core/chipseq/issues/318)] Update `bowtie2/align` module to fix issue when downloading its singularity image. -- [[#320](https://github.com/nf-core/chipseq/issues/320)] Fix samplesheet control column in documentation examples. -- [[#328](https://github.com/nf-core/chipseq/issues/328)] Modify documentation to clarify that is necessary to provide the `--read_length` when `--genome` is set and `--macs_gsize` has not provided. +- [[#318](https://github.com/nf-core/chipseq/issues/318)] - Update `bowtie2/align` module to fix issue when downloading its singularity image. +- [[#320](https://github.com/nf-core/chipseq/issues/320)] - Fix samplesheet control column in documentation examples. +- [[#328](https://github.com/nf-core/chipseq/issues/328)] - Modify documentation to clarify that is necessary to provide the `--read_length` when `--genome` is set and `--macs_gsize` has not provided. - Remove `enable_conda` param from local modules. - Fix the path where `chromap` index is stored when `--save_reference` is set. - Fix untar of `chromap` index when using `--chromap_index` param. -- [nf-core/tools#2286](https://github.com/nf-core/tools/issues/2286) Set default container registry outside profile scope. +- [nf-core/tools#2286](https://github.com/nf-core/tools/issues/2286) - Set default container registry outside profile scope. +- [[#343](https://github.com/nf-core/chipseq/issues/343)] - Provide replicate information explicitly in samplesheet. ### Software dependencies From a06cf7cbd0597bc382d70bed97a39a5db7300885 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 21 Jun 2023 09:50:20 +0200 Subject: [PATCH 081/201] Add narrow_peak input channel value to macs2_consensus --- modules/local/macs2_consensus.nf | 13 ++++++++----- workflows/chipseq.nf | 3 ++- 2 files changed, 10 insertions(+), 6 deletions(-) diff --git a/modules/local/macs2_consensus.nf b/modules/local/macs2_consensus.nf index b8a5abd7..eb373d2c 100644 --- a/modules/local/macs2_consensus.nf +++ b/modules/local/macs2_consensus.nf @@ -12,6 +12,7 @@ process MACS2_CONSENSUS { input: tuple val(meta), path(peaks) + val is_narrow_peak output: tuple val(meta), path("*.bed") , emit: bed @@ -26,11 +27,12 @@ process MACS2_CONSENSUS { task.ext.when == null || task.ext.when script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ - def prefix = task.ext.prefix ?: "${meta.id}" - def peak_type = params.narrow_peak ? 'narrowPeak' : 'broadPeak' - def mergecols = params.narrow_peak ? (2..10).join(',') : (2..9).join(',') - def collapsecols = params.narrow_peak ? (['collapse']*9).join(',') : (['collapse']*8).join(',') - def expandparam = params.narrow_peak ? '--is_narrow_peak' : '' + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def peak_type = is_narrow_peak ? 'narrowPeak' : 'broadPeak' + def mergecols = is_narrow_peak ? (2..10).join(',') : (2..9).join(',') + def collapsecols = is_narrow_peak ? (['collapse']*9).join(',') : (['collapse']*8).join(',') + def expandparam = is_narrow_peak ? '--is_narrow_peak' : '' """ sort -T '.' -k1,1 -k2,2n ${peaks.collect{it.toString()}.sort().join(' ')} \\ | mergeBed -c $mergecols -o $collapsecols > ${prefix}.txt @@ -40,6 +42,7 @@ process MACS2_CONSENSUS { ${peaks.collect{it.toString()}.sort().join(',').replaceAll("_peaks.${peak_type}","")} \\ ${prefix}.boolean.txt \\ --min_replicates $params.min_reps_consensus \\ + $args \\ $expandparam awk -v FS='\t' -v OFS='\t' 'FNR > 1 { print \$1, \$2, \$3, \$4, "0", "+" }' ${prefix}.boolean.txt > ${prefix}.bed diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 1390e114..d2faa5b2 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -604,7 +604,8 @@ workflow CHIPSEQ { // MODULE: Generate consensus peaks across samples // MACS2_CONSENSUS ( - ch_antibody_peaks + ch_antibody_peaks, + params.narrow_peak ) ch_macs2_consensus_bed_lib = MACS2_CONSENSUS.out.bed ch_macs2_consensus_txt_lib = MACS2_CONSENSUS.out.txt From 809312394d600e92e8a0e27468367f324f5bb3d6 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 21 Jun 2023 09:54:38 +0200 Subject: [PATCH 082/201] Update test_full with new version of samplesheet --- conf/test_full.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/test_full.config b/conf/test_full.config index 28cb9c98..a9478183 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -17,7 +17,7 @@ params { config_profile_description = 'Full test dataset to check pipeline function' // Input data for full size test - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/samplesheet/v2.0/samplesheet_full.csv' + input = 'https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/samplesheet/v2.1/samplesheet_full.csv' // Used to calculate --macs_gsize read_length = 50 From 2630bc7bb5e920c0e65c7db44b127c37b071ad20 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 22 Jun 2023 11:34:14 +0200 Subject: [PATCH 083/201] Update usage to cover replicate and control_replicate columns --- docs/usage.md | 108 +++++++++++++++++++++++++++++--------------------- 1 file changed, 62 insertions(+), 46 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index db940f51..252145b5 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -12,66 +12,82 @@ You will need to create a samplesheet with information about the samples you wou --input '[path to samplesheet file]' ``` -### Multiple runs of the same library +### Multiple replicates + +The `sample` identifier should be identical when you have multiple replicates from the same experimental group, just increment the `replicate` identifier appropriately. The first replicate value for any given experimental group must be 1. + +The `antibody` column is required to separate the downstream consensus peak merging for different antibodies. Its not advisable to generate a consensus peak set across different antibodies especially if their binding patterns are inherently different e.g. narrow transcription factors and broad histone marks. -The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will perform the alignments in parallel, and subsequently merge them before further analysis. Below is an example where the samples called `WT_BCATENIN_IP_REP2` and `WT_INPUT_REP2` have been re-sequenced multiple times: +The `control` column should be the `sample` identifier for the controls for any given IP. This column together with the `control_replicate` column will set the corresponding control for each of the samples in the table. ```console -sample,fastq_1,fastq_2,antibody,control -WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP1 -WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 -WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 -WT_BCATENIN_IP_REP2,BLA203A25_S16_L003_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 -WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP3 -WT_INPUT_REP1,BLA203A6_S32_L006_R1_001.fastq.gz,,, -WT_INPUT_REP2,BLA203A30_S21_L001_R1_001.fastq.gz,,, -WT_INPUT_REP2,BLA203A30_S21_L002_R1_001.fastq.gz,,, -WT_INPUT_REP3,BLA203A31_S21_L003_R1_001.fastq.gz,,, +group,fastq_1,fastq_2,replicate,antibody,control,control_replicate +WT_BCATENIN_IP,BLA203A1_S27_L006_R1_001.fastq.gz,,1,BCATENIN,WT_INPUT,1 +WT_BCATENIN_IP,BLA203A25_S16_L002_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A49_S40_L001_R1_001.fastq.gz,,3,BCATENIN,WT_INPUT,3 +WT_INPUT,BLA203A6_S32_L006_R1_001.fastq.gz,,1,,, +WT_INPUT,BLA203A30_S21_L002_R1_001.fastq.gz,,2,,, +WT_INPUT,BLA203A31_S21_L003_R1_001.fastq.gz,,3,,, ``` -### Full design +### Multiple runs of the same library -The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 5 columns to match those defined in the table below. +Both the `sample` and `replicate` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will perform the alignments in parallel, and subsequently merge them before further analysis. Below is an example where the samples called `WT_BCATENIN_IP` and `WT_INPUT` have been re-sequenced multiple times: -The `antibody` column is required to separate the downstream consensus peak merging for different antibodies. Its not advisable to generate a consensus peak set across different antibodies especially if their binding patterns are inherently different e.g. narrow transcription factors and broad histone marks. +```console +sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate +WT_BCATENIN_IP,BLA203A1_S27_L006_R1_001.fastq.gz,,1,BCATENIN,WT_INPUT,1 +WT_BCATENIN_IP,BLA203A25_S16_L001_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A25_S16_L002_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A25_S16_L003_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A49_S40_L001_R1_001.fastq.gz,,3,BCATENIN,WT_INPUT,3 +WT_INPUT,BLA203A6_S32_L006_R1_001.fastq.gz,,1,,, +WT_INPUT,BLA203A30_S21_L001_R1_001.fastq.gz,,2,,, +WT_INPUT,BLA203A30_S21_L002_R1_001.fastq.gz,,2,,, +WT_INPUT,BLA203A31_S21_L003_R1_001.fastq.gz,,3,,, +``` + +### Full design -The `control` column should be the `sample` identifier for the controls for any given IP. +The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 7 columns to match those defined in the table below. -A final design file may look something like the one below. This is for two antibodies and associated controls, where the `WT_BCATENIN_IP_REP2` and `NAIVE_BCATENIN_IP_REP2` samples have been sequenced twice: +A final design file may look something like the one below. This is for two antibodies and associated controls, where the second replicate of the `WT_BCATENIN_IP` and `NAIVE_BCATENIN_IP` samples have been sequenced twice: ```console -sample,fastq_1,fastq_2,antibody,control -WT_BCATENIN_IP_REP1,BLA203A1_S27_L006_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP1 -WT_BCATENIN_IP_REP2,BLA203A25_S16_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 -WT_BCATENIN_IP_REP2,BLA203A25_S16_L002_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP2 -WT_BCATENIN_IP_REP3,BLA203A49_S40_L001_R1_001.fastq.gz,,BCATENIN,WT_INPUT_REP3 -NAIVE_BCATENIN_IP_REP1,BLA203A7_S60_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP1 -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP2 -NAIVE_BCATENIN_IP_REP2,BLA203A43_S34_L002_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP2 -NAIVE_BCATENIN_IP_REP3,BLA203A64_S55_L001_R1_001.fastq.gz,,BCATENIN,NAIVE_INPUT_REP3 -WT_TCF4_IP_REP1,BLA203A3_S29_L006_R1_001.fastq.gz,,TCF4,WT_INPUT_REP1 -WT_TCF4_IP_REP2,BLA203A27_S18_L001_R1_001.fastq.gz,,TCF4,WT_INPUT_REP2 -WT_TCF4_IP_REP3,BLA203A51_S42_L001_R1_001.fastq.gz,,TCF4,WT_INPUT_REP3 -NAIVE_TCF4_IP_REP1,BLA203A9_S62_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT_REP1 -NAIVE_TCF4_IP_REP2,BLA203A45_S36_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT_REP2 -NAIVE_TCF4_IP_REP3,BLA203A66_S57_L001_R1_001.fastq.gz,,TCF4,NAIVE_INPUT_REP3 -WT_INPUT_REP1,BLA203A6_S32_L006_R1_001.fastq.gz,,, -WT_INPUT_REP2,BLA203A30_S21_L001_R1_001.fastq.gz,,, -WT_INPUT_REP3,BLA203A31_S21_L003_R1_001.fastq.gz,,, -NAIVE_INPUT_REP1,BLA203A12_S3_L001_R1_001.fastq.gz,,, -NAIVE_INPUT_REP2,BLA203A48_S39_L001_R1_001.fastq.gz,,, -NAIVE_INPUT_REP3,BLA203A49_S1_L006_R1_001.fastq.gz,,, +sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate +WT_BCATENIN_IP,BLA203A1_S27_L006_R1_001.fastq.gz,,1,BCATENIN,WT_INPUT,1 +WT_BCATENIN_IP,BLA203A25_S16_L001_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A25_S16_L002_R1_001.fastq.gz,,2,BCATENIN,WT_INPUT,2 +WT_BCATENIN_IP,BLA203A49_S40_L001_R1_001.fastq.gz,,3,BCATENIN,WT_INPUT,3 +NAIVE_BCATENIN_IP,BLA203A7_S60_L001_R1_001.fastq.gz,,1,BCATENIN,NAIVE_INPUT,1 +NAIVE_BCATENIN_IP,BLA203A43_S34_L001_R1_001.fastq.gz,,2,BCATENIN,NAIVE_INPUT,2 +NAIVE_BCATENIN_IP,BLA203A43_S34_L002_R1_001.fastq.gz,,2,BCATENIN,NAIVE_INPUT,2 +NAIVE_BCATENIN_IP,BLA203A64_S55_L001_R1_001.fastq.gz,,3,BCATENIN,NAIVE_INPUT,3 +WT_TCF4_IP,BLA203A3_S29_L006_R1_001.fastq.gz,,1,TCF4,WT_INPUT,1 +WT_TCF4_IP,BLA203A27_S18_L001_R1_001.fastq.gz,,2,TCF4,WT_INPUT,2 +WT_TCF4_IP,BLA203A51_S42_L001_R1_001.fastq.gz,,3,TCF4,WT_INPUT,3 +NAIVE_TCF4_IP,BLA203A9_S62_L001_R1_001.fastq.gz,,1,TCF4,NAIVE_INPUT,1 +NAIVE_TCF4_IP,BLA203A45_S36_L001_R1_001.fastq.gz,,2,TCF4,NAIVE_INPUT,2 +NAIVE_TCF4_IP,BLA203A66_S57_L001_R1_001.fastq.gz,,3,TCF4,NAIVE_INPUT,3 +WT_INPUT,BLA203A6_S32_L006_R1_001.fastq.gz,,1,,, +WT_INPUT,BLA203A30_S21_L001_R1_001.fastq.gz,,2,,, +WT_INPUT,BLA203A31_S21_L003_R1_001.fastq.gz,,3,,, +NAIVE_INPUT,BLA203A12_S3_L001_R1_001.fastq.gz,,1,,, +NAIVE_INPUT,BLA203A48_S39_L001_R1_001.fastq.gz,,2,,, +NAIVE_INPUT,BLA203A49_S1_L006_R1_001.fastq.gz,,3,,, ``` | Column | Description | -| ---------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `antibody` | Antibody name. This is required to segregate downstream analysis for different antibodies. Required when `control` is specified. | -| `control` | Sample name for control sample. | - -Example design files have been provided with the pipeline for [paired-end](../assets/samplesheet_pe.csv) and [single-end](../assets/samplesheet_se.csv) data. +| ----------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `replicate` | Integer representing replicate number. This will be identical for re-sequenced libraries. Must start from `1..`. | +| `antibody` | Antibody name. This is required to segregate downstream analysis for different antibodies. Required when `control` is specified. | +| `control` | Sample name for control sample. | +| `control_replicate` | Integer representing replicate number for control sample. | + +Example design files have bee_n provided with the pipeline for [paired-end](../assets/samplesheet_pe.csv) and [single-end](../assets/samplesheet_se.csv) data. > **NB:** The `group` and `replicate` columns were replaced with a single `sample` column as of v2.0 of the pipeline. The `sample` column is essentially a concatenation of the `group` and `replicate` columns. If all values of `sample` have the same number of underscores, fields defined by these underscore-separated names may be used in the PCA plots produced by the pipeline, to regain the ability to represent different groupings. From da7ecafa87f9c67f33255ceb072ea521c2341457 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 22 Jun 2023 11:36:42 +0200 Subject: [PATCH 084/201] Make prettier happy --- docs/usage.md | 20 ++++++++++---------- 1 file changed, 10 insertions(+), 10 deletions(-) diff --git a/docs/usage.md b/docs/usage.md index 252145b5..10524491 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -51,7 +51,7 @@ WT_INPUT,BLA203A31_S21_L003_R1_001.fastq.gz,,3,,, The pipeline will auto-detect whether a sample is single- or paired-end using the information provided in the samplesheet. The samplesheet can have as many columns as you desire, however, there is a strict requirement for the first 7 columns to match those defined in the table below. -A final design file may look something like the one below. This is for two antibodies and associated controls, where the second replicate of the `WT_BCATENIN_IP` and `NAIVE_BCATENIN_IP` samples have been sequenced twice: +A final design file may look something like the one below. This is for two antibodies and associated controls, where the second replicate of the `WT_BCATENIN_IP` and `NAIVE_BCATENIN_IP` samples have been sequenced twice: ```console sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate @@ -77,15 +77,15 @@ NAIVE_INPUT,BLA203A48_S39_L001_R1_001.fastq.gz,,2,,, NAIVE_INPUT,BLA203A49_S1_L006_R1_001.fastq.gz,,3,,, ``` -| Column | Description | -| ----------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | -| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | -| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | -| `replicate` | Integer representing replicate number. This will be identical for re-sequenced libraries. Must start from `1..`. | -| `antibody` | Antibody name. This is required to segregate downstream analysis for different antibodies. Required when `control` is specified. | -| `control` | Sample name for control sample. | -| `control_replicate` | Integer representing replicate number for control sample. | +| Column | Description | +| ------------------- | -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | +| `sample` | Custom sample name. This entry will be identical for multiple sequencing libraries/runs from the same sample. Spaces in sample names are automatically converted to underscores (`_`). | +| `fastq_1` | Full path to FastQ file for Illumina short reads 1. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `fastq_2` | Full path to FastQ file for Illumina short reads 2. File has to be gzipped and have the extension ".fastq.gz" or ".fq.gz". | +| `replicate` | Integer representing replicate number. This will be identical for re-sequenced libraries. Must start from `1..`. | +| `antibody` | Antibody name. This is required to segregate downstream analysis for different antibodies. Required when `control` is specified. | +| `control` | Sample name for control sample. | +| `control_replicate` | Integer representing replicate number for control sample. | Example design files have bee_n provided with the pipeline for [paired-end](../assets/samplesheet_pe.csv) and [single-end](../assets/samplesheet_se.csv) data. From d99f2fe60330010d33a9ac606886184ca126f77d Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Bj=C3=B6rn=20Langer?= <61791748+bjlang@users.noreply.github.com> Date: Thu, 22 Jun 2023 12:58:01 +0200 Subject: [PATCH 085/201] Update usage.md --- docs/usage.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/usage.md b/docs/usage.md index 10524491..6cab8bfe 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -16,7 +16,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifier should be identical when you have multiple replicates from the same experimental group, just increment the `replicate` identifier appropriately. The first replicate value for any given experimental group must be 1. -The `antibody` column is required to separate the downstream consensus peak merging for different antibodies. Its not advisable to generate a consensus peak set across different antibodies especially if their binding patterns are inherently different e.g. narrow transcription factors and broad histone marks. +The `antibody` column is required to separate the downstream consensus peak merging for different antibodies. It is not advisable to generate a consensus peak set across different antibodies especially if their binding patterns are inherently different e.g. narrow transcription factors and broad histone marks. The `control` column should be the `sample` identifier for the controls for any given IP. This column together with the `control_replicate` column will set the corresponding control for each of the samples in the table. From 18bdef84e8d6f69e86660dd1d13917fded8634b8 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 22 Jun 2023 14:01:00 +0200 Subject: [PATCH 086/201] Remove dump --- workflows/chipseq.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index d2faa5b2..fc914a51 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -599,7 +599,7 @@ workflow CHIPSEQ { [ meta_new, peaks ] } .set { ch_antibody_peaks } - ch_antibody_peaks.dump() + // // MODULE: Generate consensus peaks across samples // From 36f189621b80c9e29ca12856cabd3038891c247c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 22 Jun 2023 23:55:54 +0200 Subject: [PATCH 087/201] Update modules --- modules.json | 267 ++++++++++++------ modules/nf-core/bowtie2/align/main.nf | 35 ++- modules/nf-core/bowtie2/align/meta.yml | 6 +- modules/nf-core/bowtie2/build/main.nf | 14 +- modules/nf-core/bwa/index/main.nf | 2 +- modules/nf-core/bwa/mem/main.nf | 2 +- modules/nf-core/chromap/chromap/main.nf | 2 +- modules/nf-core/chromap/index/main.nf | 2 +- .../custom/dumpsoftwareversions/main.nf | 2 +- .../custom/dumpsoftwareversions/meta.yml | 2 + .../templates/dumpsoftwareversions.py | 0 modules/nf-core/custom/getchromsizes/main.nf | 2 +- .../nf-core/deeptools/computematrix/main.nf | 2 +- .../nf-core/deeptools/plotfingerprint/main.nf | 2 +- modules/nf-core/deeptools/plotheatmap/main.nf | 2 +- modules/nf-core/deeptools/plotprofile/main.nf | 2 +- modules/nf-core/fastqc/main.nf | 2 +- modules/nf-core/gffread/main.nf | 2 +- modules/nf-core/gunzip/main.nf | 2 +- modules/nf-core/gunzip/meta.yml | 1 + modules/nf-core/homer/annotatepeaks/main.nf | 2 +- modules/nf-core/khmer/uniquekmers/main.nf | 2 +- modules/nf-core/macs2/callpeak/main.nf | 2 +- modules/nf-core/multiqc/main.nf | 2 +- modules/nf-core/multiqc/meta.yml | 3 +- modules/nf-core/phantompeakqualtools/main.nf | 2 +- .../picard/collectmultiplemetrics/main.nf | 2 +- modules/nf-core/picard/markduplicates/main.nf | 6 +- .../nf-core/picard/markduplicates/meta.yml | 11 + modules/nf-core/picard/mergesamfiles/main.nf | 2 +- modules/nf-core/preseq/lcextrap/main.nf | 2 +- modules/nf-core/samtools/flagstat/main.nf | 6 +- modules/nf-core/samtools/idxstats/main.nf | 6 +- modules/nf-core/samtools/index/main.nf | 6 +- modules/nf-core/samtools/sort/main.nf | 14 +- modules/nf-core/samtools/stats/main.nf | 8 +- modules/nf-core/samtools/stats/meta.yml | 10 +- modules/nf-core/subread/featurecounts/main.nf | 2 +- modules/nf-core/trimgalore/main.nf | 2 +- modules/nf-core/ucsc/bedgraphtobigwig/main.nf | 2 +- modules/nf-core/umitools/extract/main.nf | 2 +- modules/nf-core/untar/main.nf | 2 +- modules/nf-core/untar/meta.yml | 1 + modules/nf-core/untarfiles/main.nf | 2 +- modules/nf-core/untarfiles/meta.yml | 3 +- .../nf-core/bam_markduplicates_picard/main.nf | 2 +- .../bam_markduplicates_picard/meta.yml | 1 + .../nf-core/bam_sort_stats_samtools/main.nf | 2 +- .../nf-core/bam_sort_stats_samtools/meta.yml | 1 + .../nf-core/bam_stats_samtools/main.nf | 4 +- .../nf-core/bam_stats_samtools/meta.yml | 1 + .../nf-core/fastq_align_bowtie2/main.nf | 4 +- subworkflows/nf-core/fastq_align_bwa/meta.yml | 1 + .../nf-core/fastq_align_chromap/main.nf | 2 +- .../nf-core/fastq_align_chromap/meta.yml | 1 + .../fastq_fastqc_umitools_trimgalore/meta.yml | 13 +- 56 files changed, 327 insertions(+), 158 deletions(-) mode change 100644 => 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py diff --git a/modules.json b/modules.json index 3f822ac4..f781edf2 100644 --- a/modules.json +++ b/modules.json @@ -7,178 +7,260 @@ "nf-core": { "bowtie2/align": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["fastq_align_bowtie2", "modules"] + "git_sha": "fe54581f8bed20e4c4a51c616c93fd3379d89820", + "installed_by": [ + "modules", + "fastq_align_bowtie2" + ] }, "bowtie2/build": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["fastq_align_bwa", "modules"] + "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", + "installed_by": [ + "modules", + "fastq_align_bwa" + ] }, "chromap/chromap": { "branch": "master", - "git_sha": "b7f0dbc07af00c0ccdc7c2df06efee8786e976ac", - "installed_by": ["fastq_align_chromap", "modules"] + "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", + "installed_by": [ + "modules", + "fastq_align_chromap" + ] }, "chromap/index": { "branch": "master", - "git_sha": "d6b7b1f108dab88b0269a4331767c36a1a8da960", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "7101db4432d3268b7fcb5b8f75fa0a022dc5561b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "custom/getchromsizes": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "deeptools/computematrix": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "deeptools/plotfingerprint": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "deeptools/plotheatmap": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "deeptools/plotprofile": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "fastqc": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules", "fastq_fastqc_umitools_trimgalore"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "gffread": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", + "installed_by": [ + "modules" + ] }, "homer/annotatepeaks": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "khmer/uniquekmers": { "branch": "master", - "git_sha": "2f398a6342db61e7ab0f4cb4d395eed74b65db7c", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "macs2/callpeak": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", - "git_sha": "ee80d14721e76e2e079103b8dcd5d57129e584ba", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "phantompeakqualtools": { "branch": "master", - "git_sha": "2f398a6342db61e7ab0f4cb4d395eed74b65db7c", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "picard/collectmultiplemetrics": { "branch": "master", - "git_sha": "75027bf77472b1f4fd2cdd7e46f83119dfb0f2c6", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "picard/markduplicates": { "branch": "master", - "git_sha": "75027bf77472b1f4fd2cdd7e46f83119dfb0f2c6", - "installed_by": ["modules", "bam_markduplicates_picard"] + "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "installed_by": [ + "modules", + "bam_markduplicates_picard" + ] }, "picard/mergesamfiles": { "branch": "master", - "git_sha": "75027bf77472b1f4fd2cdd7e46f83119dfb0f2c6", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "preseq/lcextrap": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "samtools/flagstat": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules", "bam_stats_samtools"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules", + "bam_stats_samtools" + ] }, "samtools/idxstats": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules", "bam_stats_samtools"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules", + "bam_stats_samtools" + ] }, "samtools/index": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules", "bam_markduplicates_picard", "bam_sort_stats_samtools"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules", + "bam_markduplicates_picard", + "bam_sort_stats_samtools" + ] }, "samtools/sort": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["bam_sort_stats_samtools", "modules"] + "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9", + "installed_by": [ + "modules", + "bam_sort_stats_samtools" + ] }, "samtools/stats": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules", "bam_stats_samtools"] + "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "installed_by": [ + "modules", + "bam_stats_samtools" + ] }, "subread/featurecounts": { "branch": "master", - "git_sha": "c8e35eb2055c099720a75538d1b8adb3fb5a464c", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "trimgalore": { "branch": "master", - "git_sha": "64a3dbc0a30a94cdaed7869d8e34fbb85e886614", - "installed_by": ["modules", "fastq_fastqc_umitools_trimgalore"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "ucsc/bedgraphtobigwig": { "branch": "master", - "git_sha": "0f8a77ff00e65eaeebc509b8156eaa983192474b", - "installed_by": ["modules"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "modules" + ] }, "umitools/extract": { "branch": "master", - "git_sha": "ffe2f5865f608848e440a52b73c304ea79aaf818", - "installed_by": ["fastq_fastqc_umitools_trimgalore"] + "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "installed_by": [ + "fastq_fastqc_umitools_trimgalore" + ] }, "untar": { "branch": "master", - "git_sha": "cc1f997fab6d8fde5dc0e6e2a310814df5b53ce7", - "installed_by": ["modules"] + "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", + "installed_by": [ + "modules" + ] }, "untarfiles": { "branch": "master", - "git_sha": "2498e243c8c30c7125661e62fc777917d8e343b9", - "installed_by": ["modules"] + "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", + "installed_by": [ + "modules" + ] } } }, @@ -186,41 +268,58 @@ "nf-core": { "bam_markduplicates_picard": { "branch": "master", - "git_sha": "6f1697c121719dedde9e0537b6ed6a9cb8c13583", - "installed_by": ["subworkflows"] + "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", + "installed_by": [ + "subworkflows" + ] }, "bam_sort_stats_samtools": { "branch": "master", - "git_sha": "3911652a6b24249358f79e8b8466338d63efb2a2", - "installed_by": ["fastq_align_bowtie2", "fastq_align_chromap", "fastq_align_bwa"] + "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "installed_by": [ + "fastq_align_bowtie2", + "fastq_align_chromap", + "fastq_align_bwa" + ] }, "bam_stats_samtools": { "branch": "master", - "git_sha": "b4b7f89e7fd6d2293f0c176213f710e0bcdaf19e", - "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] + "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "installed_by": [ + "bam_sort_stats_samtools", + "bam_markduplicates_picard" + ] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "ac75f79157ecc64283a2b3a559f1ba90bc0f2259", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "fastq_align_bwa": { "branch": "master", - "git_sha": "901fab507683647b43e7032f3ae9e4c234eb68eb", - "installed_by": ["subworkflows"] + "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", + "installed_by": [ + "subworkflows" + ] }, "fastq_align_chromap": { "branch": "master", - "git_sha": "ac75f79157ecc64283a2b3a559f1ba90bc0f2259", - "installed_by": ["subworkflows"] + "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "installed_by": [ + "subworkflows" + ] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", - "git_sha": "100caf3506850ffcc04953f724a97f422940c377", - "installed_by": ["subworkflows"] + "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", + "installed_by": [ + "subworkflows" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf index 3d851866..a77114d2 100644 --- a/modules/nf-core/bowtie2/align/main.nf +++ b/modules/nf-core/bowtie2/align/main.nf @@ -5,7 +5,7 @@ process BOWTIE2_ALIGN { conda "bioconda::bowtie2=2.4.4 bioconda::samtools=1.16.1 conda-forge::pigz=2.6" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' : - 'quay.io/biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" + 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" input: tuple val(meta) , path(reads) @@ -14,10 +14,10 @@ process BOWTIE2_ALIGN { val sort_bam output: - tuple val(meta), path("*.bam") , emit: bam - tuple val(meta), path("*.log") , emit: log - tuple val(meta), path("*fastq.gz"), emit: fastq, optional:true - path "versions.yml" , emit: versions + tuple val(meta), path("*.{bam,sam}"), emit: aligned + tuple val(meta), path("*.log") , emit: log + tuple val(meta), path("*fastq.gz") , emit: fastq, optional:true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -38,6 +38,8 @@ process BOWTIE2_ALIGN { } def samtools_command = sort_bam ? 'sort' : 'view' + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension = (args2 ==~ extension_pattern) ? (args2 =~ extension_pattern)[0][2].toLowerCase() : "bam" """ INDEX=`find -L ./ -name "*.rev.1.bt2" | sed "s/\\.rev.1.bt2\$//"` @@ -51,7 +53,7 @@ process BOWTIE2_ALIGN { $unaligned \\ $args \\ 2> ${prefix}.bowtie2.log \\ - | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.bam - + | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.${extension} - if [ -f ${prefix}.unmapped.fastq.1.gz ]; then mv ${prefix}.unmapped.fastq.1.gz ${prefix}.unmapped_1.fastq.gz @@ -68,4 +70,25 @@ process BOWTIE2_ALIGN { pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) END_VERSIONS """ + + stub: + def args2 = task.ext.args2 ?: "" + def prefix = task.ext.prefix ?: "${meta.id}" + def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ + def extension = (args2 ==~ extension_pattern) ? (args2 =~ extension_pattern)[0][2].toLowerCase() : "bam" + + """ + touch ${prefix}.${extension} + touch ${prefix}.bowtie2.log + touch ${prefix}.unmapped_1.fastq.gz + touch ${prefix}.unmapped_2.fastq.gz + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + pigz: \$( pigz --version 2>&1 | sed 's/pigz //g' ) + END_VERSIONS + """ + } diff --git a/modules/nf-core/bowtie2/align/meta.yml b/modules/nf-core/bowtie2/align/meta.yml index c8e9a001..60d04c12 100644 --- a/modules/nf-core/bowtie2/align/meta.yml +++ b/modules/nf-core/bowtie2/align/meta.yml @@ -46,10 +46,10 @@ input: description: use samtools sort (true) or samtools view (false) pattern: "true or false" output: - - bam: + - aligned: type: file - description: Output BAM file containing read alignments - pattern: "*.{bam}" + description: Output BAM/SAM file containing read alignments + pattern: "*.{bam,sam}" - versions: type: file description: File containing software versions diff --git a/modules/nf-core/bowtie2/build/main.nf b/modules/nf-core/bowtie2/build/main.nf index 551893af..069d9c12 100644 --- a/modules/nf-core/bowtie2/build/main.nf +++ b/modules/nf-core/bowtie2/build/main.nf @@ -5,7 +5,7 @@ process BOWTIE2_BUILD { conda "bioconda::bowtie2=2.4.4" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bowtie2:2.4.4--py39hbb4e92a_0' : - 'quay.io/biocontainers/bowtie2:2.4.4--py39hbb4e92a_0' }" + 'biocontainers/bowtie2:2.4.4--py39hbb4e92a_0' }" input: tuple val(meta), path(fasta) @@ -27,4 +27,16 @@ process BOWTIE2_BUILD { bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//') END_VERSIONS """ + + stub: + """ + mkdir bowtie2 + touch bowtie2/${fasta.baseName}.{1..4}.bt2 + touch bowtie2/${fasta.baseName}.rev.{1,2}.bt2 + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bowtie2: \$(echo \$(bowtie2 --version 2>&1) | sed 's/^.*bowtie2-align-s version //; s/ .*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/bwa/index/main.nf b/modules/nf-core/bwa/index/main.nf index 7ccf3110..8d2e56d9 100644 --- a/modules/nf-core/bwa/index/main.nf +++ b/modules/nf-core/bwa/index/main.nf @@ -5,7 +5,7 @@ process BWA_INDEX { conda "bioconda::bwa=0.7.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bwa:0.7.17--hed695b0_7' : - 'quay.io/biocontainers/bwa:0.7.17--hed695b0_7' }" + 'biocontainers/bwa:0.7.17--hed695b0_7' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/bwa/mem/main.nf b/modules/nf-core/bwa/mem/main.nf index cbf34c25..d2f85daf 100644 --- a/modules/nf-core/bwa/mem/main.nf +++ b/modules/nf-core/bwa/mem/main.nf @@ -5,7 +5,7 @@ process BWA_MEM { conda "bioconda::bwa=0.7.17 bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:219b6c272b25e7e642ae3ff0bf0c5c81a5135ab4-0' : - 'quay.io/biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:219b6c272b25e7e642ae3ff0bf0c5c81a5135ab4-0' }" + 'biocontainers/mulled-v2-fe8faa35dbf6dc65a0f7f5d4ea12e31a79f73e40:219b6c272b25e7e642ae3ff0bf0c5c81a5135ab4-0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/chromap/chromap/main.nf b/modules/nf-core/chromap/chromap/main.nf index 69a4bc1b..a39302e3 100644 --- a/modules/nf-core/chromap/chromap/main.nf +++ b/modules/nf-core/chromap/chromap/main.nf @@ -5,7 +5,7 @@ process CHROMAP_CHROMAP { conda "bioconda::chromap=0.2.4 bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:5b2e433ab8b3d1ef098fc944b567fd98caa23f56-0' : - 'quay.io/biocontainers/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:5b2e433ab8b3d1ef098fc944b567fd98caa23f56-0' }" + 'biocontainers/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:5b2e433ab8b3d1ef098fc944b567fd98caa23f56-0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/chromap/index/main.nf b/modules/nf-core/chromap/index/main.nf index 25ee6a7e..fb3773f8 100644 --- a/modules/nf-core/chromap/index/main.nf +++ b/modules/nf-core/chromap/index/main.nf @@ -5,7 +5,7 @@ process CHROMAP_INDEX { conda "bioconda::chromap=0.2.4" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/chromap:0.2.4--hd03093a_0' : - 'quay.io/biocontainers/chromap:0.2.4--hd03093a_0' }" + 'biocontainers/chromap:0.2.4--hd03093a_0' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 800a6099..ebc87273 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -5,7 +5,7 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { conda "bioconda::multiqc=1.14" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index 60b546a0..c32657de 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,7 +1,9 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: - custom + - dump - version tools: - custom: diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py old mode 100644 new mode 100755 diff --git a/modules/nf-core/custom/getchromsizes/main.nf b/modules/nf-core/custom/getchromsizes/main.nf index 580f87fe..060a2e88 100644 --- a/modules/nf-core/custom/getchromsizes/main.nf +++ b/modules/nf-core/custom/getchromsizes/main.nf @@ -5,7 +5,7 @@ process CUSTOM_GETCHROMSIZES { conda "bioconda::samtools=1.16.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'biocontainers/samtools:1.16.1--h6899075_1' }" input: tuple val(meta), path(fasta) diff --git a/modules/nf-core/deeptools/computematrix/main.nf b/modules/nf-core/deeptools/computematrix/main.nf index 024b8797..e77e2839 100644 --- a/modules/nf-core/deeptools/computematrix/main.nf +++ b/modules/nf-core/deeptools/computematrix/main.nf @@ -5,7 +5,7 @@ process DEEPTOOLS_COMPUTEMATRIX { conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : - 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" + 'biocontainers/deeptools:3.5.1--py_0' }" input: tuple val(meta), path(bigwig) diff --git a/modules/nf-core/deeptools/plotfingerprint/main.nf b/modules/nf-core/deeptools/plotfingerprint/main.nf index 233b3bae..3159e941 100644 --- a/modules/nf-core/deeptools/plotfingerprint/main.nf +++ b/modules/nf-core/deeptools/plotfingerprint/main.nf @@ -5,7 +5,7 @@ process DEEPTOOLS_PLOTFINGERPRINT { conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : - 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" + 'biocontainers/deeptools:3.5.1--py_0' }" input: tuple val(meta), path(bams), path(bais) diff --git a/modules/nf-core/deeptools/plotheatmap/main.nf b/modules/nf-core/deeptools/plotheatmap/main.nf index 3b171b73..58309eea 100644 --- a/modules/nf-core/deeptools/plotheatmap/main.nf +++ b/modules/nf-core/deeptools/plotheatmap/main.nf @@ -5,7 +5,7 @@ process DEEPTOOLS_PLOTHEATMAP { conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : - 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" + 'biocontainers/deeptools:3.5.1--py_0' }" input: tuple val(meta), path(matrix) diff --git a/modules/nf-core/deeptools/plotprofile/main.nf b/modules/nf-core/deeptools/plotprofile/main.nf index b5fa57df..b1ab454d 100644 --- a/modules/nf-core/deeptools/plotprofile/main.nf +++ b/modules/nf-core/deeptools/plotprofile/main.nf @@ -5,7 +5,7 @@ process DEEPTOOLS_PLOTPROFILE { conda "bioconda::deeptools=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/deeptools:3.5.1--py_0' : - 'quay.io/biocontainers/deeptools:3.5.1--py_0' }" + 'biocontainers/deeptools:3.5.1--py_0' }" input: tuple val(meta), path(matrix) diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9ae58381..07d5e433 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -5,7 +5,7 @@ process FASTQC { conda "bioconda::fastqc=0.11.9" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'quay.io/biocontainers/fastqc:0.11.9--0' }" + 'biocontainers/fastqc:0.11.9--0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/gffread/main.nf b/modules/nf-core/gffread/main.nf index b1a8996f..f4472b0e 100644 --- a/modules/nf-core/gffread/main.nf +++ b/modules/nf-core/gffread/main.nf @@ -5,7 +5,7 @@ process GFFREAD { conda "bioconda::gffread=0.12.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0' : - 'quay.io/biocontainers/gffread:0.12.1--h8b12597_0' }" + 'biocontainers/gffread:0.12.1--h8b12597_0' }" input: path gff diff --git a/modules/nf-core/gunzip/main.nf b/modules/nf-core/gunzip/main.nf index d906034c..e7189d2f 100644 --- a/modules/nf-core/gunzip/main.nf +++ b/modules/nf-core/gunzip/main.nf @@ -5,7 +5,7 @@ process GUNZIP { conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'ubuntu:20.04' }" + 'nf-core/ubuntu:20.04' }" input: tuple val(meta), path(archive) diff --git a/modules/nf-core/gunzip/meta.yml b/modules/nf-core/gunzip/meta.yml index 2e0e4054..4cdcdf4c 100644 --- a/modules/nf-core/gunzip/meta.yml +++ b/modules/nf-core/gunzip/meta.yml @@ -3,6 +3,7 @@ description: Compresses and decompresses files. keywords: - gunzip - compression + - decompression tools: - gunzip: description: | diff --git a/modules/nf-core/homer/annotatepeaks/main.nf b/modules/nf-core/homer/annotatepeaks/main.nf index 865ad81c..cfc140f5 100644 --- a/modules/nf-core/homer/annotatepeaks/main.nf +++ b/modules/nf-core/homer/annotatepeaks/main.nf @@ -6,7 +6,7 @@ process HOMER_ANNOTATEPEAKS { conda "bioconda::homer=4.11" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/homer:4.11--pl526hc9558a2_3' : - 'quay.io/biocontainers/homer:4.11--pl526hc9558a2_3' }" + 'biocontainers/homer:4.11--pl526hc9558a2_3' }" input: tuple val(meta), path(peak) diff --git a/modules/nf-core/khmer/uniquekmers/main.nf b/modules/nf-core/khmer/uniquekmers/main.nf index 02746ae1..9576034f 100644 --- a/modules/nf-core/khmer/uniquekmers/main.nf +++ b/modules/nf-core/khmer/uniquekmers/main.nf @@ -5,7 +5,7 @@ process KHMER_UNIQUEKMERS { conda "bioconda::khmer=3.0.0a3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/khmer:3.0.0a3--py37haa7609a_2' : - 'quay.io/biocontainers/khmer:3.0.0a3--py37haa7609a_2' }" + 'biocontainers/khmer:3.0.0a3--py37haa7609a_2' }" input: path fasta diff --git a/modules/nf-core/macs2/callpeak/main.nf b/modules/nf-core/macs2/callpeak/main.nf index feca0425..cbef7838 100644 --- a/modules/nf-core/macs2/callpeak/main.nf +++ b/modules/nf-core/macs2/callpeak/main.nf @@ -5,7 +5,7 @@ process MACS2_CALLPEAK { conda "bioconda::macs2=2.2.7.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/macs2:2.2.7.1--py38h4a8c8d9_3' : - 'quay.io/biocontainers/macs2:2.2.7.1--py38h4a8c8d9_3' }" + 'biocontainers/macs2:2.2.7.1--py38h4a8c8d9_3' }" input: tuple val(meta), path(ipbam), path(controlbam) diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 4b604749..1fc387be 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -4,7 +4,7 @@ process MULTIQC { conda "bioconda::multiqc=1.14" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'quay.io/biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index ebc29b27..f93b5ee5 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json name: MultiQC description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: @@ -37,7 +38,7 @@ output: description: MultiQC report file pattern: "multiqc_report.html" - data: - type: dir + type: directory description: MultiQC data dir pattern: "multiqc_data" - plots: diff --git a/modules/nf-core/phantompeakqualtools/main.nf b/modules/nf-core/phantompeakqualtools/main.nf index 27daac4c..edcdf2ce 100644 --- a/modules/nf-core/phantompeakqualtools/main.nf +++ b/modules/nf-core/phantompeakqualtools/main.nf @@ -6,7 +6,7 @@ process PHANTOMPEAKQUALTOOLS { conda "bioconda::phantompeakqualtools=1.2.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/phantompeakqualtools:1.2.2--0' : - 'quay.io/biocontainers/phantompeakqualtools:1.2.2--0' }" + 'biocontainers/phantompeakqualtools:1.2.2--0' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/picard/collectmultiplemetrics/main.nf b/modules/nf-core/picard/collectmultiplemetrics/main.nf index ed88dbe7..91fe9170 100644 --- a/modules/nf-core/picard/collectmultiplemetrics/main.nf +++ b/modules/nf-core/picard/collectmultiplemetrics/main.nf @@ -5,7 +5,7 @@ process PICARD_COLLECTMULTIPLEMETRICS { conda "bioconda::picard=3.0.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'quay.io/biocontainers/picard:3.0.0--hdfd78af_1' }" + 'biocontainers/picard:3.0.0--hdfd78af_1' }" input: tuple val(meta) , path(bam), path(bai) diff --git a/modules/nf-core/picard/markduplicates/main.nf b/modules/nf-core/picard/markduplicates/main.nf index 1fe6ee2d..facd7efb 100644 --- a/modules/nf-core/picard/markduplicates/main.nf +++ b/modules/nf-core/picard/markduplicates/main.nf @@ -5,12 +5,12 @@ process PICARD_MARKDUPLICATES { conda "bioconda::picard=3.0.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'quay.io/biocontainers/picard:3.0.0--hdfd78af_1' }" + 'biocontainers/picard:3.0.0--hdfd78af_1' }" input: tuple val(meta), path(bam) - path fasta - path fai + tuple val(meta2), path(fasta) + tuple val(meta3), path(fai) output: tuple val(meta), path("*.bam") , emit: bam diff --git a/modules/nf-core/picard/markduplicates/meta.yml b/modules/nf-core/picard/markduplicates/meta.yml index 3f2357bb..f7693d2f 100644 --- a/modules/nf-core/picard/markduplicates/meta.yml +++ b/modules/nf-core/picard/markduplicates/meta.yml @@ -25,10 +25,20 @@ input: type: file description: BAM file pattern: "*.{bam,cram,sam}" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] - fasta: type: file description: Reference genome fasta file pattern: "*.{fasta,fa}" + - meta3: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] - fai: type: file description: Reference genome fasta index @@ -58,3 +68,4 @@ output: authors: - "@drpatelh" - "@projectoriented" + - "@ramprasadn" diff --git a/modules/nf-core/picard/mergesamfiles/main.nf b/modules/nf-core/picard/mergesamfiles/main.nf index ff8d2f17..fc23ea01 100644 --- a/modules/nf-core/picard/mergesamfiles/main.nf +++ b/modules/nf-core/picard/mergesamfiles/main.nf @@ -5,7 +5,7 @@ process PICARD_MERGESAMFILES { conda "bioconda::picard=3.0.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'quay.io/biocontainers/picard:3.0.0--hdfd78af_1' }" + 'biocontainers/picard:3.0.0--hdfd78af_1' }" input: tuple val(meta), path(bams) diff --git a/modules/nf-core/preseq/lcextrap/main.nf b/modules/nf-core/preseq/lcextrap/main.nf index 38275910..12546f0a 100644 --- a/modules/nf-core/preseq/lcextrap/main.nf +++ b/modules/nf-core/preseq/lcextrap/main.nf @@ -6,7 +6,7 @@ process PRESEQ_LCEXTRAP { conda "bioconda::preseq=3.1.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/preseq:3.1.2--h445547b_2': - 'quay.io/biocontainers/preseq:3.1.2--h445547b_2' }" + 'biocontainers/preseq:3.1.2--h445547b_2' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf index 2120cd7d..eb7e72fc 100644 --- a/modules/nf-core/samtools/flagstat/main.nf +++ b/modules/nf-core/samtools/flagstat/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.16.1" + conda "bioconda::samtools=1.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf index a7b87d8b..a257d700 100644 --- a/modules/nf-core/samtools/idxstats/main.nf +++ b/modules/nf-core/samtools/idxstats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.16.1" + conda "bioconda::samtools=1.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index 8b95687a..0b20aa4b 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.16.1" + conda "bioconda::samtools=1.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf index 84c167cd..2b7753fd 100644 --- a/modules/nf-core/samtools/sort/main.nf +++ b/modules/nf-core/samtools/sort/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_SORT { tag "$meta.id" label 'process_medium' - conda "bioconda::samtools=1.16.1" + conda "bioconda::samtools=1.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: tuple val(meta), path(bam) @@ -23,7 +23,13 @@ process SAMTOOLS_SORT { def prefix = task.ext.prefix ?: "${meta.id}" if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ - samtools sort $args -@ $task.cpus -o ${prefix}.bam -T $prefix $bam + samtools sort \\ + $args \\ + -@ $task.cpus \\ + -o ${prefix}.bam \\ + -T $prefix \\ + $bam + cat <<-END_VERSIONS > versions.yml "${task.process}": samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf index 0a2a3640..4a2607de 100644 --- a/modules/nf-core/samtools/stats/main.nf +++ b/modules/nf-core/samtools/stats/main.nf @@ -2,14 +2,14 @@ process SAMTOOLS_STATS { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.16.1" + conda "bioconda::samtools=1.17" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.16.1--h6899075_1' : - 'quay.io/biocontainers/samtools:1.16.1--h6899075_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : + 'biocontainers/samtools:1.17--h00cdaf9_0' }" input: tuple val(meta), path(input), path(input_index) - path fasta + tuple val(meta2), path(fasta) output: tuple val(meta), path("*.stats"), emit: stats diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml index 1d68a5d8..90e6345f 100644 --- a/modules/nf-core/samtools/stats/meta.yml +++ b/modules/nf-core/samtools/stats/meta.yml @@ -30,9 +30,14 @@ input: type: file description: BAI/CRAI file from alignment pattern: "*.{bai,crai}" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'genome' ] - fasta: - type: optional file - description: Reference file the CRAM was created with + type: file + description: Reference file the CRAM was created with (optional) pattern: "*.{fasta,fa}" output: - meta: @@ -51,3 +56,4 @@ output: authors: - "@drpatelh" - "@FriederikeHanssen" + - "@ramprasadn" diff --git a/modules/nf-core/subread/featurecounts/main.nf b/modules/nf-core/subread/featurecounts/main.nf index cbc833fe..a524b92f 100644 --- a/modules/nf-core/subread/featurecounts/main.nf +++ b/modules/nf-core/subread/featurecounts/main.nf @@ -5,7 +5,7 @@ process SUBREAD_FEATURECOUNTS { conda "bioconda::subread=2.0.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/subread:2.0.1--hed695b0_0' : - 'quay.io/biocontainers/subread:2.0.1--hed695b0_0' }" + 'biocontainers/subread:2.0.1--hed695b0_0' }" input: tuple val(meta), path(bams), path(annotation) diff --git a/modules/nf-core/trimgalore/main.nf b/modules/nf-core/trimgalore/main.nf index dc295784..dcb77ae7 100644 --- a/modules/nf-core/trimgalore/main.nf +++ b/modules/nf-core/trimgalore/main.nf @@ -5,7 +5,7 @@ process TRIMGALORE { conda "bioconda::trim-galore=0.6.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/trim-galore:0.6.7--hdfd78af_0' : - 'quay.io/biocontainers/trim-galore:0.6.7--hdfd78af_0' }" + 'biocontainers/trim-galore:0.6.7--hdfd78af_0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf index defda3ef..054924e7 100644 --- a/modules/nf-core/ucsc/bedgraphtobigwig/main.nf +++ b/modules/nf-core/ucsc/bedgraphtobigwig/main.nf @@ -6,7 +6,7 @@ process UCSC_BEDGRAPHTOBIGWIG { conda "bioconda::ucsc-bedgraphtobigwig=377" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ucsc-bedgraphtobigwig:377--h446ed27_1' : - 'quay.io/biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }" + 'biocontainers/ucsc-bedgraphtobigwig:377--h446ed27_1' }" input: tuple val(meta), path(bedgraph) diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf index 64fdd367..2f94fa93 100644 --- a/modules/nf-core/umitools/extract/main.nf +++ b/modules/nf-core/umitools/extract/main.nf @@ -6,7 +6,7 @@ process UMITOOLS_EXTRACT { conda "bioconda::umi_tools=1.1.4" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' : - 'quay.io/biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }" + 'biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/untar/main.nf b/modules/nf-core/untar/main.nf index 3384847a..8cd1856c 100644 --- a/modules/nf-core/untar/main.nf +++ b/modules/nf-core/untar/main.nf @@ -5,7 +5,7 @@ process UNTAR { conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'ubuntu:20.04' }" + 'nf-core/ubuntu:20.04' }" input: tuple val(meta), path(archive) diff --git a/modules/nf-core/untar/meta.yml b/modules/nf-core/untar/meta.yml index ea7a3f38..db241a6e 100644 --- a/modules/nf-core/untar/meta.yml +++ b/modules/nf-core/untar/meta.yml @@ -3,6 +3,7 @@ description: Extract files. keywords: - untar - uncompress + - extract tools: - untar: description: | diff --git a/modules/nf-core/untarfiles/main.nf b/modules/nf-core/untarfiles/main.nf index 8bca09d0..59111b5f 100644 --- a/modules/nf-core/untarfiles/main.nf +++ b/modules/nf-core/untarfiles/main.nf @@ -5,7 +5,7 @@ process UNTARFILES { conda "conda-forge::sed=4.7 bioconda::grep=3.4 conda-forge::tar=1.34" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'ubuntu:20.04' }" + 'nf-core/ubuntu:20.04' }" input: tuple val(meta), path(archive) diff --git a/modules/nf-core/untarfiles/meta.yml b/modules/nf-core/untarfiles/meta.yml index e89aa3d6..098490fa 100644 --- a/modules/nf-core/untarfiles/meta.yml +++ b/modules/nf-core/untarfiles/meta.yml @@ -3,6 +3,7 @@ description: Extract files. keywords: - untar - uncompress + - files tools: - untar: description: | @@ -26,7 +27,7 @@ output: Groovy Map containing sample information e.g. [ id:'test', single_end:false ] - files: - type: list + type: string description: A list containing references to individual archive files pattern: "*/**" - versions: diff --git a/subworkflows/nf-core/bam_markduplicates_picard/main.nf b/subworkflows/nf-core/bam_markduplicates_picard/main.nf index 6e3df332..3725ea95 100644 --- a/subworkflows/nf-core/bam_markduplicates_picard/main.nf +++ b/subworkflows/nf-core/bam_markduplicates_picard/main.nf @@ -17,7 +17,7 @@ workflow BAM_MARKDUPLICATES_PICARD { ch_versions = Channel.empty() - PICARD_MARKDUPLICATES ( ch_bam, ch_fasta, ch_fai ) + PICARD_MARKDUPLICATES ( ch_bam, ch_fasta.map { [ [:], it ] }, ch_fai.map { [ [:], it ] } ) ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions.first()) SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) diff --git a/subworkflows/nf-core/bam_markduplicates_picard/meta.yml b/subworkflows/nf-core/bam_markduplicates_picard/meta.yml index 822c6132..d5e71609 100644 --- a/subworkflows/nf-core/bam_markduplicates_picard/meta.yml +++ b/subworkflows/nf-core/bam_markduplicates_picard/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json name: "bam_markduplicates_picard" description: Picard MarkDuplicates, index BAM file and run samtools stats, flagstat and idxstats keywords: diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf index 617871fe..fc1c652b 100644 --- a/subworkflows/nf-core/bam_sort_stats_samtools/main.nf +++ b/subworkflows/nf-core/bam_sort_stats_samtools/main.nf @@ -9,7 +9,7 @@ include { BAM_STATS_SAMTOOLS } from '../bam_stats_samtools/main' workflow BAM_SORT_STATS_SAMTOOLS { take: ch_bam // channel: [ val(meta), [ bam ] ] - ch_fasta // channel: [ fasta ] + ch_fasta // channel: [ val(meta), path(fasta) ] main: diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml index 131065be..8dfbd58d 100644 --- a/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml +++ b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json name: bam_sort_stats_samtools description: Sort SAM/BAM/CRAM file keywords: diff --git a/subworkflows/nf-core/bam_stats_samtools/main.nf b/subworkflows/nf-core/bam_stats_samtools/main.nf index c9d7c8b7..e80ce43a 100644 --- a/subworkflows/nf-core/bam_stats_samtools/main.nf +++ b/subworkflows/nf-core/bam_stats_samtools/main.nf @@ -9,12 +9,12 @@ include { SAMTOOLS_FLAGSTAT } from '../../../modules/nf-core/samtools/flagstat/m workflow BAM_STATS_SAMTOOLS { take: ch_bam_bai // channel: [ val(meta), path(bam), path(bai) ] - ch_fasta // channel: [ path(fasta) ] + ch_fasta // channel: [ val(meta), path(fasta) ] main: ch_versions = Channel.empty() - SAMTOOLS_STATS ( ch_bam_bai, ch_fasta ) + SAMTOOLS_STATS ( ch_bam_bai, ch_fasta.map { [ [:], it ] } ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions) SAMTOOLS_FLAGSTAT ( ch_bam_bai ) diff --git a/subworkflows/nf-core/bam_stats_samtools/meta.yml b/subworkflows/nf-core/bam_stats_samtools/meta.yml index b6072686..b05086bc 100644 --- a/subworkflows/nf-core/bam_stats_samtools/meta.yml +++ b/subworkflows/nf-core/bam_stats_samtools/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json name: bam_stats_samtools description: Produces comprehensive statistics from SAM/BAM/CRAM file keywords: diff --git a/subworkflows/nf-core/fastq_align_bowtie2/main.nf b/subworkflows/nf-core/fastq_align_bowtie2/main.nf index eaf4ac5e..e77cb2c4 100644 --- a/subworkflows/nf-core/fastq_align_bowtie2/main.nf +++ b/subworkflows/nf-core/fastq_align_bowtie2/main.nf @@ -26,11 +26,11 @@ workflow FASTQ_ALIGN_BOWTIE2 { // // Sort, index BAM file and run samtools stats, flagstat and idxstats // - BAM_SORT_STATS_SAMTOOLS ( BOWTIE2_ALIGN.out.bam, ch_fasta ) + BAM_SORT_STATS_SAMTOOLS ( BOWTIE2_ALIGN.out.aligned, ch_fasta ) ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) emit: - bam_orig = BOWTIE2_ALIGN.out.bam // channel: [ val(meta), bam ] + bam_orig = BOWTIE2_ALIGN.out.aligned // channel: [ val(meta), bam ] log_out = BOWTIE2_ALIGN.out.log // channel: [ val(meta), log ] fastq = BOWTIE2_ALIGN.out.fastq // channel: [ val(meta), fastq ] diff --git a/subworkflows/nf-core/fastq_align_bwa/meta.yml b/subworkflows/nf-core/fastq_align_bwa/meta.yml index 03f84860..548fec3f 100644 --- a/subworkflows/nf-core/fastq_align_bwa/meta.yml +++ b/subworkflows/nf-core/fastq_align_bwa/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json name: fastq_align_bwa description: Align reads to a reference genome using bwa then sort with samtools keywords: diff --git a/subworkflows/nf-core/fastq_align_chromap/main.nf b/subworkflows/nf-core/fastq_align_chromap/main.nf index 9d706c98..26b6a4be 100644 --- a/subworkflows/nf-core/fastq_align_chromap/main.nf +++ b/subworkflows/nf-core/fastq_align_chromap/main.nf @@ -27,7 +27,7 @@ workflow FASTQ_ALIGN_CHROMAP { // // Sort, index BAM file and run samtools stats, flagstat and idxstats // - BAM_SORT_STATS_SAMTOOLS(CHROMAP_CHROMAP.out.bam, ch_fasta.map { it[1] }) + BAM_SORT_STATS_SAMTOOLS(CHROMAP_CHROMAP.out.bam, ch_fasta) ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) emit: diff --git a/subworkflows/nf-core/fastq_align_chromap/meta.yml b/subworkflows/nf-core/fastq_align_chromap/meta.yml index 6d701bce..d5f59cac 100644 --- a/subworkflows/nf-core/fastq_align_chromap/meta.yml +++ b/subworkflows/nf-core/fastq_align_chromap/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json name: "fastq_align_chromap" description: Align high throughput chromatin profiles using Chromap then sort with samtools keywords: diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml index b05004d4..3b1a675c 100644 --- a/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml +++ b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json name: "fastq_fastqc_umitools_trimgalore" description: Read QC, UMI extraction and trimming keywords: @@ -49,12 +50,12 @@ input: output: - reads: - type: file - description: > - Extracted FASTQ files. | - For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | - For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. - pattern: "*.{fastq.gz}" + type: file + description: > + Extracted FASTQ files. | + For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | + For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. + pattern: "*.{fastq.gz}" - fastqc_html: type: file description: FastQC report From ea4517b6ab146f1749f1422a843e70bad85b6cb9 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 08:17:33 +0200 Subject: [PATCH 088/201] Run prettier for modules --- modules.json | 185 ++++++++++++--------------------------------------- 1 file changed, 43 insertions(+), 142 deletions(-) diff --git a/modules.json b/modules.json index f781edf2..68895b20 100644 --- a/modules.json +++ b/modules.json @@ -8,259 +8,177 @@ "bowtie2/align": { "branch": "master", "git_sha": "fe54581f8bed20e4c4a51c616c93fd3379d89820", - "installed_by": [ - "modules", - "fastq_align_bowtie2" - ] + "installed_by": ["modules", "fastq_align_bowtie2"] }, "bowtie2/build": { "branch": "master", "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", - "installed_by": [ - "modules", - "fastq_align_bwa" - ] + "installed_by": ["modules", "fastq_align_bwa"] }, "chromap/chromap": { "branch": "master", "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", - "installed_by": [ - "modules", - "fastq_align_chromap" - ] + "installed_by": ["modules", "fastq_align_chromap"] }, "chromap/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/getchromsizes": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/computematrix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "gffread": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "macs2/callpeak": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "phantompeakqualtools": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/markduplicates": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": [ - "modules", - "bam_markduplicates_picard" - ] + "installed_by": ["modules", "bam_markduplicates_picard"] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "preseq/lcextrap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules", - "bam_stats_samtools" - ] + "installed_by": ["modules", "bam_stats_samtools"] }, "samtools/idxstats": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules", - "bam_stats_samtools" - ] + "installed_by": ["modules", "bam_stats_samtools"] }, "samtools/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules", - "bam_markduplicates_picard", - "bam_sort_stats_samtools" - ] + "installed_by": ["modules", "bam_markduplicates_picard", "bam_sort_stats_samtools"] }, "samtools/sort": { "branch": "master", "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9", - "installed_by": [ - "modules", - "bam_sort_stats_samtools" - ] + "installed_by": ["modules", "bam_sort_stats_samtools"] }, "samtools/stats": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": [ - "modules", - "bam_stats_samtools" - ] + "installed_by": ["modules", "bam_stats_samtools"] }, "subread/featurecounts": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "trimgalore": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "umitools/extract": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore"] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "untarfiles": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -269,57 +187,40 @@ "bam_markduplicates_picard": { "branch": "master", "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "bam_sort_stats_samtools": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": [ - "fastq_align_bowtie2", - "fastq_align_chromap", - "fastq_align_bwa" - ] + "installed_by": ["fastq_align_bowtie2", "fastq_align_chromap", "fastq_align_bwa"] }, "bam_stats_samtools": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": [ - "bam_sort_stats_samtools", - "bam_markduplicates_picard" - ] + "installed_by": ["bam_sort_stats_samtools", "bam_markduplicates_picard"] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "ac75f79157ecc64283a2b3a559f1ba90bc0f2259", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_bwa": { "branch": "master", "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_chromap": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } } } -} \ No newline at end of file +} From 6bff0a93ef720e3ea32a66f76d8a287db1d6eefa Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 08:37:17 +0200 Subject: [PATCH 089/201] Update changelog --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 2c9b7821..5b6c2fb9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -30,7 +30,7 @@ Note, since the pipeline is now using Nextflow DSL2, each process will be run wi | `chromap` | 0.2.1 | 0.2.4 | | `multiqc` | 1.13 | 1.14 | | `picard` | 2.27.4 | 3.0.0 | -| `samtools` | 1.15.1 | 1.16.1 | +| `samtools` | 1.15.1 | 1.17 | > **NB:** Dependency has been **updated** if both old and new version information is present. > **NB:** Dependency has been **added** if just the new version information is present. From 0e90e764c567b566a5991774f53b145c58570170 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 09:14:59 +0200 Subject: [PATCH 090/201] Fix fasta input for chromap --- workflows/chipseq.nf | 5 +---- 1 file changed, 1 insertion(+), 4 deletions(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index fc914a51..33303160 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -215,10 +215,7 @@ workflow CHIPSEQ { FASTQ_ALIGN_CHROMAP ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.chromap_index, - PREPARE_GENOME.out.fasta - .map { - [ [:], it ] - }, + PREPARE_GENOME.out.fasta, [], [], [], From 4c2fcf36e9685e234d0557176f77881e9257b406 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 09:22:55 +0200 Subject: [PATCH 091/201] Fix issue with fasta (needs map in some instances and not in some others) --- .../nf-core/fastq_align_chromap/main.nf | 2 +- workflows/chipseq.nf | 29 +++++++------------ 2 files changed, 11 insertions(+), 20 deletions(-) diff --git a/subworkflows/nf-core/fastq_align_chromap/main.nf b/subworkflows/nf-core/fastq_align_chromap/main.nf index 26b6a4be..9d706c98 100644 --- a/subworkflows/nf-core/fastq_align_chromap/main.nf +++ b/subworkflows/nf-core/fastq_align_chromap/main.nf @@ -27,7 +27,7 @@ workflow FASTQ_ALIGN_CHROMAP { // // Sort, index BAM file and run samtools stats, flagstat and idxstats // - BAM_SORT_STATS_SAMTOOLS(CHROMAP_CHROMAP.out.bam, ch_fasta) + BAM_SORT_STATS_SAMTOOLS(CHROMAP_CHROMAP.out.bam, ch_fasta.map { it[1] }) ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) emit: diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 33303160..bdf5783f 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -215,7 +215,10 @@ workflow CHIPSEQ { FASTQ_ALIGN_CHROMAP ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.chromap_index, - PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fasta + .map { + [ [:], it ] + }, [], [], [], @@ -420,19 +423,11 @@ workflow CHIPSEQ { .set { ch_genome_bam_bai } ch_genome_bam_bai + .combine(ch_genome_bam_bai) .map { - meta, bam, bai -> - meta.control ? null : [ meta.id, [ bam ] , [ bai ] ] + meta1, bam1, bai1, meta2, bam2, bai2 -> + meta1.control == meta2.id ? [ meta1, [ bam1, bam2 ], [ bai1, bai2 ] ] : null } - .set { ch_control_bam_bai } - - ch_genome_bam_bai - .map { - meta, bam, bai -> - meta.control ? [ meta.control, meta, [ bam ], [ bai ] ] : null - } - .combine(ch_control_bam_bai, by: 0) - .map { it -> [ it[1] , it[2] + it[4], it[3] + it[5] ] } .set { ch_ip_control_bam_bai } // @@ -487,10 +482,7 @@ workflow CHIPSEQ { MACS2_CALLPEAK .out .peak - .filter { - meta, peaks -> - peaks.size() > 0 - } + .filter { meta, peaks -> peaks.size() > 0 } .set { ch_macs2_peaks } // Create channels: [ meta, ip_bam, peaks ] @@ -572,7 +564,7 @@ workflow CHIPSEQ { ch_deseq2_clustering_multiqc = Channel.empty() if (!params.skip_consensus_peaks) { // Create channels: [ meta , [ peaks ] ] - // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] + // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] ch_macs2_peaks .map { meta, peak -> @@ -601,8 +593,7 @@ workflow CHIPSEQ { // MODULE: Generate consensus peaks across samples // MACS2_CONSENSUS ( - ch_antibody_peaks, - params.narrow_peak + ch_antibody_peaks ) ch_macs2_consensus_bed_lib = MACS2_CONSENSUS.out.bed ch_macs2_consensus_txt_lib = MACS2_CONSENSUS.out.txt From 6331a76450e2ea196d0efad73629413ab016e822 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 09:59:17 +0200 Subject: [PATCH 092/201] Pass narrow peak parameter to macs2_consensus --- workflows/chipseq.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index bdf5783f..363f9de0 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -593,7 +593,8 @@ workflow CHIPSEQ { // MODULE: Generate consensus peaks across samples // MACS2_CONSENSUS ( - ch_antibody_peaks + ch_antibody_peaks, + params.narrow_peak ) ch_macs2_consensus_bed_lib = MACS2_CONSENSUS.out.bed ch_macs2_consensus_txt_lib = MACS2_CONSENSUS.out.txt From 81c26f11f76fd589511772c84f25011856cbec52 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 10:46:16 +0200 Subject: [PATCH 093/201] Revert changes in chipseq pushed by mistake --- workflows/chipseq.nf | 21 ++++++++++++++++----- 1 file changed, 16 insertions(+), 5 deletions(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 363f9de0..fc914a51 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -423,11 +423,19 @@ workflow CHIPSEQ { .set { ch_genome_bam_bai } ch_genome_bam_bai - .combine(ch_genome_bam_bai) .map { - meta1, bam1, bai1, meta2, bam2, bai2 -> - meta1.control == meta2.id ? [ meta1, [ bam1, bam2 ], [ bai1, bai2 ] ] : null + meta, bam, bai -> + meta.control ? null : [ meta.id, [ bam ] , [ bai ] ] } + .set { ch_control_bam_bai } + + ch_genome_bam_bai + .map { + meta, bam, bai -> + meta.control ? [ meta.control, meta, [ bam ], [ bai ] ] : null + } + .combine(ch_control_bam_bai, by: 0) + .map { it -> [ it[1] , it[2] + it[4], it[3] + it[5] ] } .set { ch_ip_control_bam_bai } // @@ -482,7 +490,10 @@ workflow CHIPSEQ { MACS2_CALLPEAK .out .peak - .filter { meta, peaks -> peaks.size() > 0 } + .filter { + meta, peaks -> + peaks.size() > 0 + } .set { ch_macs2_peaks } // Create channels: [ meta, ip_bam, peaks ] @@ -564,7 +575,7 @@ workflow CHIPSEQ { ch_deseq2_clustering_multiqc = Channel.empty() if (!params.skip_consensus_peaks) { // Create channels: [ meta , [ peaks ] ] - // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] + // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] ch_macs2_peaks .map { meta, peak -> From 1d995677435274aff97d43b2ef77191344e5bfd2 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 11:51:17 +0200 Subject: [PATCH 094/201] Make fasta available through IGV run --- conf/modules.config | 16 ++++++++++------ modules/local/igv.nf | 1 + subworkflows/local/prepare_genome.nf | 6 ------ 3 files changed, 11 insertions(+), 12 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 06a1e5a6..2d57aa3a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -718,12 +718,16 @@ if (!params.skip_igv) { process { withName: 'IGV' { publishDir = [ - path: { [ - "${params.outdir}/igv", - params.narrow_peak? '/narrowPeak' : '/broadPeak' - ].join('') }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + [ + path: { "${params.outdir}/igv/${params.narrow_peak? '/narrowPeak' : '/broadPeak' }" }, + mode: params.publish_dir_mode, + pattern: '*.{txt,xml}' + ], + [ + path: { "${params.outdir}/genome" }, + mode: params.publish_dir_mode, + pattern: '*.{fa,fasta}' + ] ] } } diff --git a/modules/local/igv.nf b/modules/local/igv.nf index 59a2d2e8..f1f24325 100644 --- a/modules/local/igv.nf +++ b/modules/local/igv.nf @@ -20,6 +20,7 @@ process IGV { output: path "*files.txt" , emit: txt path "*.xml" , emit: xml + path fasta , emit: fasta path "versions.yml", emit: versions script: // scripts are bundled with the pipeline in nf-core/chipseq/bin/ diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index dd16e1f5..b67b12c5 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -44,12 +44,6 @@ workflow PREPARE_GENOME { ch_fasta = file(params.fasta) } - // Make fasta file available if reference saved or IGV is run - if (params.save_reference || !params.skip_igv) { - file("${params.outdir}/genome/").mkdirs() - ch_fasta.copyTo("${params.outdir}/genome/") - } - // // Uncompress GTF annotation file or create from GFF3 if required // From c21f05e16170b217a59df737a3ba63a7d84e04b7 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 12:47:14 +0200 Subject: [PATCH 095/201] Fix blacklist and other instances where a parameter is used to create a channel --- subworkflows/local/prepare_genome.nf | 16 +++++++++------- 1 file changed, 9 insertions(+), 7 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index b67b12c5..4520b61e 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -41,7 +41,8 @@ workflow PREPARE_GENOME { ch_fasta = GUNZIP_FASTA ( [ [:], params.fasta ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { - ch_fasta = file(params.fasta) + // ch_fasta = file(params.fasta) + ch_fasta = Channel.value(file(params.fasta)) } // @@ -101,14 +102,15 @@ workflow PREPARE_GENOME { ch_gene_bed = GUNZIP_GENE_BED ( [ [:], params.gene_bed ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_GENE_BED.out.versions) } else { - ch_gene_bed = file(params.gene_bed) + ch_gene_bed = Channel.value(file(params.gene_bed)) } } // // Create chromosome sizes file // - ch_chrom_sizes = CUSTOM_GETCHROMSIZES ( [ [:], ch_fasta ] ).sizes.map{ it[1] } + CUSTOM_GETCHROMSIZES ( ch_fasta.map { [ [:], it ] } ) + ch_chrom_sizes = CUSTOM_GETCHROMSIZES.out.sizes.map { it[1] } ch_fai = CUSTOM_GETCHROMSIZES.out.fai.map{ it[1] } ch_versions = ch_versions.mix(CUSTOM_GETCHROMSIZES.out.versions) @@ -138,7 +140,7 @@ workflow PREPARE_GENOME { ch_bwa_index = file(params.bwa_index) } } else { - ch_bwa_index = BWA_INDEX ( [ [:], ch_fasta ] ).index + ch_bwa_index = BWA_INDEX ( ch_fasta.map { [ [:], it ] } ).index ch_versions = ch_versions.mix(BWA_INDEX.out.versions) } } @@ -156,7 +158,7 @@ workflow PREPARE_GENOME { ch_bowtie2_index = [ [:], file(params.bowtie2_index) ] } } else { - ch_bowtie2_index = BOWTIE2_BUILD ( [ [:], ch_fasta ] ).index + ch_bowtie2_index = BOWTIE2_BUILD ( ch_fasta.map { [ [:], it ] } ).index ch_versions = ch_versions.mix(BOWTIE2_BUILD.out.versions) } } @@ -174,7 +176,7 @@ workflow PREPARE_GENOME { ch_chromap_index = [ [:], file(params.chromap_index) ] } } else { - ch_chromap_index = CHROMAP_INDEX ( [ [:], ch_fasta ] ).index + ch_chromap_index = CHROMAP_INDEX ( ch_fasta.map { [ [:], it ] } ).index ch_versions = ch_versions.mix(CHROMAP_INDEX.out.versions) } } @@ -189,7 +191,7 @@ workflow PREPARE_GENOME { ch_star_index = UNTAR_STAR_INDEX ( [ [:], params.star_index ] ).untar.map{ it[1] } ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { - ch_star_index = file(params.star_index) + ch_star_index = Channel.value(file(params.star_index)) } } else { ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index From 77aac1df868210c379f59b9ce21aeaac6ff19516 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 14:08:35 +0200 Subject: [PATCH 096/201] Remove commented line --- subworkflows/local/prepare_genome.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 4520b61e..bd7033cb 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -41,7 +41,6 @@ workflow PREPARE_GENOME { ch_fasta = GUNZIP_FASTA ( [ [:], params.fasta ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { - // ch_fasta = file(params.fasta) ch_fasta = Channel.value(file(params.fasta)) } From 05ab0543a450455c0080f5b8c00a461374b3be8c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 14:20:24 +0200 Subject: [PATCH 097/201] Remove parameter narrow_peak from plot_macs2_qc --- modules/local/plot_macs2_qc.nf | 2 +- workflows/chipseq.nf | 3 ++- 2 files changed, 3 insertions(+), 2 deletions(-) diff --git a/modules/local/plot_macs2_qc.nf b/modules/local/plot_macs2_qc.nf index 92f400d5..6a06e355 100644 --- a/modules/local/plot_macs2_qc.nf +++ b/modules/local/plot_macs2_qc.nf @@ -16,7 +16,7 @@ process PLOT_MACS2_QC { script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ def args = task.ext.args ?: '' - def peak_type = params.narrow_peak ? 'narrowPeak' : 'broadPeak' + def peak_type = is_narrow_peak ? 'narrowPeak' : 'broadPeak' """ plot_macs2_qc.r \\ -i ${peaks.join(',')} \\ diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index fc914a51..ec966a8d 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -549,7 +549,8 @@ workflow CHIPSEQ { // MODULE: MACS2 QC plots with R // PLOT_MACS2_QC ( - ch_macs2_peaks.collect{it[1]} + ch_macs2_peaks.collect{it[1]}, + is_narrow_peak ) ch_versions = ch_versions.mix(PLOT_MACS2_QC.out.versions) From 4c484fba4ef502830e680d0760fd127bb82fb946 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 23 Jun 2023 17:39:40 +0200 Subject: [PATCH 098/201] Remove first not necessary --- workflows/chipseq.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index ec966a8d..43694c60 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -205,7 +205,7 @@ workflow CHIPSEQ { ch_samtools_stats = FASTQ_ALIGN_BOWTIE2.out.stats ch_samtools_flagstat = FASTQ_ALIGN_BOWTIE2.out.flagstat ch_samtools_idxstats = FASTQ_ALIGN_BOWTIE2.out.idxstats - ch_versions = ch_versions.mix(FASTQ_ALIGN_BOWTIE2.out.versions.first()) + ch_versions = ch_versions.mix(FASTQ_ALIGN_BOWTIE2.out.versions) } // @@ -229,7 +229,7 @@ workflow CHIPSEQ { ch_samtools_stats = FASTQ_ALIGN_CHROMAP.out.stats ch_samtools_flagstat = FASTQ_ALIGN_CHROMAP.out.flagstat ch_samtools_idxstats = FASTQ_ALIGN_CHROMAP.out.idxstats - ch_versions = ch_versions.mix(FASTQ_ALIGN_CHROMAP.out.versions.first()) + ch_versions = ch_versions.mix(FASTQ_ALIGN_CHROMAP.out.versions) } // @@ -274,7 +274,7 @@ workflow CHIPSEQ { PICARD_MERGESAMFILES ( ch_sort_bam ) - ch_versions = ch_versions.mix(PICARD_MERGESAMFILES.out.versions.first().ifEmpty(null)) + ch_versions = ch_versions.mix(PICARD_MERGESAMFILES.out.versions.first()) // // SUBWORKFLOW: Mark duplicates & filter BAM files after merging From e88fdcdcf2748b56d0b2fbd7b43e9c696937f579 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 26 Jun 2023 08:26:53 +0200 Subject: [PATCH 099/201] Fix typo --- workflows/chipseq.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 43694c60..01ff8545 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -550,7 +550,7 @@ workflow CHIPSEQ { // PLOT_MACS2_QC ( ch_macs2_peaks.collect{it[1]}, - is_narrow_peak + params.narrow_peak ) ch_versions = ch_versions.mix(PLOT_MACS2_QC.out.versions) From 5bb757de74fbc0f7f1ad5182f1c6a01e4180623e Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 26 Jun 2023 08:49:22 +0200 Subject: [PATCH 100/201] Add is_narrow_peak to plot_macs2_qc --- modules/local/plot_macs2_qc.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/modules/local/plot_macs2_qc.nf b/modules/local/plot_macs2_qc.nf index 6a06e355..2915b739 100644 --- a/modules/local/plot_macs2_qc.nf +++ b/modules/local/plot_macs2_qc.nf @@ -8,6 +8,7 @@ process PLOT_MACS2_QC { input: path peaks + val is_narrow_peak output: path '*.txt' , emit: txt From cc578b6452b64d360d4ad28353c0eba0dcce0ecc Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 26 Jun 2023 18:53:48 +0200 Subject: [PATCH 101/201] Add ext.when, remove params to local modules and rearrange modules config --- conf/modules.config | 188 ++++++++---------- modules/local/bam_remove_orphans.nf | 3 + modules/local/bedtools_genomecov.nf | 6 +- modules/local/deseq2_qc.nf | 8 +- modules/local/frip_score.nf | 3 + modules/local/genome_blacklist_regions.nf | 3 + modules/local/gtf2bed.nf | 3 + modules/local/igv.nf | 3 + modules/local/multiqc.nf | 3 + modules/local/multiqc_custom_peaks.nf | 3 + .../multiqc_custom_phantompeakqualtools.nf | 3 + modules/local/plot_homer_annotatepeaks.nf | 3 + modules/local/plot_macs2_qc.nf | 3 + modules/local/samplesheet_check.nf | 2 +- modules/local/star_genomegenerate.nf | 3 + 15 files changed, 124 insertions(+), 113 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 2d57aa3a..a112e27d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -21,7 +21,8 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:INPUT_CHECK:SAMPLESHEET_CHECK' { + withName: 'SAMPLESHEET_CHECK' { + ext.args = 'samplesheet.valid.csv' publishDir = [ path: { "${params.outdir}/pipeline_info" }, mode: params.publish_dir_mode, @@ -36,6 +37,10 @@ process { pattern: '*_versions.yml' ] } + + withName: 'KHMER_UNIQUEKMERS' { + publishDir = [ enabled: false ] + } } // @@ -62,7 +67,7 @@ process { ] } - withName: 'BWA_INDEX|BOWTIE2_BUILD|STAR_GENOMEGENERATE' { + withName: 'BWA_INDEX|BOWTIE2_BUILD|STAR_GENOMEGENERATE|CHROMAP_INDEX' { publishDir = [ path: { "${params.outdir}/genome/index" }, mode: params.publish_dir_mode, @@ -71,15 +76,6 @@ process { ] } - withName: 'UNTAR_CHROMAP_INDEX|CHROMAP_INDEX' { - publishDir = [ - path: { "${params.outdir}/genome/index/chromap" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - enabled: params.save_reference - ] - } - withName: 'GFFREAD' { ext.args = '--keep-exon-attrs -F -T' publishDir = [ @@ -111,7 +107,8 @@ process { publishDir = [ path: { "${params.outdir}/genome" }, mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + enabled: params.save_reference ] } } @@ -258,6 +255,14 @@ if (params.aligner == 'bowtie2') { if (params.aligner == 'chromap') { process { + withName: 'CHROMAP_INDEX' { + publishDir = [ + path: { "${params.outdir}/genome/${params.aligner}/index" }, + mode: params.publish_dir_mode, + saveAs: { filename -> filename.equals('versions.yml') ? null : filename } + ] + } + withName: CHROMAP_CHROMAP { ext.args = '-l 2000 --low-mem --SAM' ext.prefix = { "${meta.id}.Lb" } @@ -311,7 +316,12 @@ process { withName: 'PICARD_MERGESAMFILES' { ext.args = '--SORT_ORDER coordinate --VALIDATION_STRINGENCY LENIENT --TMP_DIR tmp' ext.prefix = { "${meta.id}.mLb.sorted" } - publishDir = [ enabled: false ] + publishDir = [ + path: { "${params.outdir}/${params.aligner}/merged_library" }, + mode: params.publish_dir_mode, + pattern: '*.bam', + enabled: params.save_align_intermeds + ] } withName: '.*:BAM_MARKDUPLICATES_PICARD:PICARD_MARKDUPLICATES' { @@ -350,7 +360,6 @@ process { ] } - // Should only be published when paired end data is used and save_align_intermeds is true withName: 'BAMTOOLS_FILTER' { ext.args = { [ @@ -359,34 +368,41 @@ process { params.keep_multi_map ? '' : '-q 1' ].join(' ').trim() } - ext.prefix = { meta.single_end ? "${meta.id}.mLb.noPublish" : "${meta.id}.mLb.flT.sorted" } //TODO check with atacseq + ext.prefix = { meta.single_end ? "${meta.id}.mLb.clN.sorted" : "${meta.id}.mLb.flT.sorted" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, + path: { "${params.outdir}/${params.aligner}/merged_library" }, mode: params.publish_dir_mode, - pattern: '*.mLb.flT.sorted.bam', - enabled: params.save_align_intermeds + pattern: '*.bam', + saveAs: { (meta.single_end || params.save_align_intermeds) ? "${it}" : null } ] } withName: 'BAM_REMOVE_ORPHANS' { ext.args = '--only_fr_pairs' ext.prefix = { "${meta.id}.mLb.clN" } - publishDir = [ enabled: false ] + publishDir = [ + path: { "${params.outdir}/${params.aligner}/merged_library" }, + mode: params.publish_dir_mode, + pattern: '*.bam', + enabled: params.save_align_intermeds + ] } withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:SAMTOOLS_SORT' { - ext.prefix = { "${meta.id}.mLb.clN.sorted" } + ext.args = '-n' + ext.prefix = { "${meta.id}.mLb.flT.name_sorted" } publishDir = [ path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, mode: params.publish_dir_mode, - pattern: '*.bam' + pattern: '*.bam', + enabled: params.save_align_intermeds ] } withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:SAMTOOLS_INDEX' { ext.prefix = { "${meta.id}.mLb.clN.sorted" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, + path: { "${params.outdir}/${params.aligner}/merged_library" }, mode: params.publish_dir_mode, pattern: '*.{bai,csi}' ] @@ -395,7 +411,7 @@ process { withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:BAM_STATS_SAMTOOLS:.*' { ext.prefix = { "${meta.id}.mLb.clN.sorted.bam" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/samtools_stats" }, + path: { "${params.outdir}/${params.aligner}/merged_library/samtools_stats" }, mode: params.publish_dir_mode, pattern: "*.{stats,flagstat,idxstats}" ] @@ -404,7 +420,7 @@ process { withName: 'PHANTOMPEAKQUALTOOLS' { ext.args2 = { "-p=$task.cpus" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/phantompeakqualtools" }, + path: { "${params.outdir}/${params.aligner}/merged_library/phantompeakqualtools" }, mode: params.publish_dir_mode, pattern: "*.{out,pdf}" ] @@ -412,25 +428,37 @@ process { withName: 'MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS' { publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/phantompeakqualtools" }, + path: { "${params.outdir}/${params.aligner}/merged_library/phantompeakqualtools" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } withName: 'BEDTOOLS_GENOMECOV' { - ext.prefix = { "${meta.id}" } + ext.args = { + [ + (meta.single_end && params.fragment_size > 0) ? "-fs ${params.fragment_size}" : '' + ].join(' ').trim() + } + ext.prefix = { "${meta.id}.mLB.clN" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/bigwig/scale" }, - mode: params.publish_dir_mode, - pattern: "*.txt" + [ + path: { "${params.outdir}/${params.aligner}/merged_library/bigwig" }, + mode: params.publish_dir_mode, + pattern: "*.bigWig" + ], + [ + path: { "${params.outdir}/${params.aligner}/merged_library/bigwig/scale" }, + mode: params.publish_dir_mode, + pattern: "*.txt" + ] ] } withName: 'UCSC_BEDGRAPHTOBIGWIG' { - ext.prefix = { "${meta.id}" } + ext.prefix = { "${meta.id}..mLb.clN" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/bigwig" }, + path: { "${params.outdir}/${params.aligner}/merged_library/bigwig" }, mode: params.publish_dir_mode, pattern: "*.bigWig" ] @@ -444,12 +472,12 @@ if (!params.skip_picard_metrics) { ext.prefix = { "${meta.id}.mLb.clN.sorted" } publishDir = [ [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/picard_metrics" }, + path: { "${params.outdir}/${params.aligner}/merged_library/picard_metrics" }, mode: params.publish_dir_mode, pattern: "*_metrics" ], [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/picard_metrics/pdf" }, + path: { "${params.outdir}/${params.aligner}/merged_library/picard_metrics/pdf" }, mode: params.publish_dir_mode, pattern: "*.pdf" ] @@ -462,9 +490,9 @@ if (!params.skip_preseq) { process { withName: 'PRESEQ_LCEXTRAP' { ext.args = '-verbose -bam -seed 1' - ext.prefix = { "${meta.id}.mLb.clN" } + ext.prefix = { "${meta.id}.mLb.mkD" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/preseq" }, + path: { "${params.outdir}/${params.aligner}/merged_library/preseq" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -475,10 +503,10 @@ if (!params.skip_preseq) { if (!params.skip_plot_profile) { process { withName: 'DEEPTOOLS_COMPUTEMATRIX' { - ext.args = 'scale-regions --regionBodyLength 1000 --beforeRegionStartLength 3000 --afterRegionStartLength 3000 --skipZeros --smartLabels' + ext.args = 'scale-regions --regionBodyLength 1000 --beforeRegionStartLength 3000 --afterRegionStartLength 3000 --missingDataAsZero --skipZeros --smartLabels' ext.prefix = { "${meta.id}.mLb.clN" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/deepTools/plotProfile" }, + path: { "${params.outdir}/${params.aligner}/merged_library/deepTools/plotProfile" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -487,7 +515,7 @@ if (!params.skip_plot_profile) { withName: 'DEEPTOOLS_PLOTPROFILE' { ext.prefix = { "${meta.id}.mLb.clN" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/deepTools/plotProfile" }, + path: { "${params.outdir}/${params.aligner}/merged_library/deepTools/plotProfile" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -496,7 +524,7 @@ if (!params.skip_plot_profile) { withName: 'DEEPTOOLS_PLOTHEATMAP' { ext.prefix = { "${meta.id}.mLb.clN" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/deepTools/plotProfile" }, + path: { "${params.outdir}/${params.aligner}/merged_library/deepTools/plotProfile" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -504,12 +532,6 @@ if (!params.skip_plot_profile) { } } -process { - withName: 'KHMER_UNIQUEKMERS' { - publishDir = [ enabled: false ] - } -} - if (!params.skip_plot_fingerprint) { process { withName: 'DEEPTOOLS_PLOTFINGERPRINT' { @@ -520,7 +542,7 @@ if (!params.skip_plot_fingerprint) { ].join(' ').trim() } ext.prefix = { "${meta.id}.mLb.clN" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/deepTools/plotFingerprint" }, + path: { "${params.outdir}/${params.aligner}/merged_library/deepTools/plotFingerprint" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -536,11 +558,11 @@ process { params.save_macs_pileup ? '--bdg --SPMR' : '', params.macs_fdr ? "--qvalue ${params.macs_fdr}" : '', params.macs_pvalue ? "--pvalue ${params.macs_pvalue}" : '', - params.aligner == "chromap" ? "--format BAM" : '' + params.aligner == "chromap" ? "--format BAM" : '' //TODO check if not needed anymore with new chromap versions ].join(' ').trim() publishDir = [ path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", + "${params.outdir}/${params.aligner}/merged_library/macs2", params.narrow_peak? '/narrowPeak' : '/broadPeak' ].join('') }, mode: params.publish_dir_mode, @@ -551,22 +573,14 @@ process { withName: 'FRIP_SCORE' { ext.args = '-bed -c -f 0.20' publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/qc' - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/qc" }, enabled: false ] } withName: 'MULTIQC_CUSTOM_PEAKS' { publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/qc' - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak} ? '/narrowPeak' : '/broadPeak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -579,10 +593,7 @@ if (!params.skip_peak_annotation) { ext.args = '-gid' ext.prefix = { "${meta.id}_peaks" } publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak' - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak' }" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -594,11 +605,7 @@ if (!params.skip_peak_annotation) { withName: 'PLOT_MACS2_QC' { ext.args = '-o ./ -p macs2_peak' publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/qc' - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -608,11 +615,7 @@ if (!params.skip_peak_annotation) { ext.args = '-o ./' ext.prefix = 'macs2_annotatePeaks' publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/qc' - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -627,12 +630,7 @@ if (!params.skip_consensus_peaks) { ext.when = { meta.multiple_groups || meta.replicates_exist } ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/consensus', - "/${meta.id}" - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -642,12 +640,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-F SAF -O --fracOverlap 0.2' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/consensus', - "/${meta.id}" - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -660,12 +653,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-gid' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/consensus', - "/${meta.id}" - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -674,12 +662,7 @@ if (!params.skip_consensus_peaks) { withName: 'ANNOTATE_BOOLEAN_PEAKS' { ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/consensus', - "/${meta.id}" - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -699,13 +682,7 @@ if (!params.skip_consensus_peaks) { ].join(' ').trim() ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/mergedLibrary/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak', - '/consensus', - "/${meta.id}", - '/deseq2' - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}/deseq2" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -738,10 +715,7 @@ if (!params.skip_multiqc) { withName: 'MULTIQC' { ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' publishDir = [ - path: { [ - "${params.outdir}/multiqc", - params.narrow_peak? '/narrowPeak' : '/broadPeak' - ].join('') }, + path: { "${params.outdir}/multiqc/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/modules/local/bam_remove_orphans.nf b/modules/local/bam_remove_orphans.nf index 95a56c60..d6e221aa 100644 --- a/modules/local/bam_remove_orphans.nf +++ b/modules/local/bam_remove_orphans.nf @@ -17,6 +17,9 @@ process BAM_REMOVE_ORPHANS { tuple val(meta), path("${prefix}.bam"), emit: bam path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/local/bedtools_genomecov.nf b/modules/local/bedtools_genomecov.nf index 2f38c06c..eb3d0dcc 100644 --- a/modules/local/bedtools_genomecov.nf +++ b/modules/local/bedtools_genomecov.nf @@ -15,11 +15,13 @@ process BEDTOOLS_GENOMECOV { tuple val(meta), path("*.txt") , emit: scale_factor path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: + def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" - def pe = meta.single_end ? '' : '-pc' - def extend = (meta.single_end && params.fragment_size > 0) ? "-fs ${params.fragment_size}" : '' """ SCALE_FACTOR=\$(grep '[0-9] mapped (' $flagstat | awk '{print 1000000/\$1}') echo \$SCALE_FACTOR > ${prefix}.scale_factor.txt diff --git a/modules/local/deseq2_qc.nf b/modules/local/deseq2_qc.nf index fe389300..d9b96e11 100644 --- a/modules/local/deseq2_qc.nf +++ b/modules/local/deseq2_qc.nf @@ -26,10 +26,12 @@ process DESEQ2_QC { path "size_factors" , optional:true, emit: size_factors path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: - def args = task.ext.args ?: '' - def peak_type = params.narrow_peak ? 'narrowPeak' : 'broadPeak' - def prefix = task.ext.prefix ?: "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" """ deseq2_qc.r \\ --count_file $counts \\ diff --git a/modules/local/frip_score.nf b/modules/local/frip_score.nf index ffcc6e2d..28133e0c 100644 --- a/modules/local/frip_score.nf +++ b/modules/local/frip_score.nf @@ -14,6 +14,9 @@ process FRIP_SCORE { tuple val(meta), path("*.txt"), emit: txt path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" diff --git a/modules/local/genome_blacklist_regions.nf b/modules/local/genome_blacklist_regions.nf index 4421fb6c..fcb8451c 100644 --- a/modules/local/genome_blacklist_regions.nf +++ b/modules/local/genome_blacklist_regions.nf @@ -17,6 +17,9 @@ process GENOME_BLACKLIST_REGIONS { path '*.bed' , emit: bed path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: def file_out = "${sizes.simpleName}.include_regions.bed" if (blacklist) { diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf index 91e29062..8a8e7bf9 100644 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -14,6 +14,9 @@ process GTF2BED { path '*.bed' , emit: bed path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ """ gtf2bed \\ diff --git a/modules/local/igv.nf b/modules/local/igv.nf index f1f24325..cf02fd9e 100644 --- a/modules/local/igv.nf +++ b/modules/local/igv.nf @@ -23,6 +23,9 @@ process IGV { path fasta , emit: fasta path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: // scripts are bundled with the pipeline in nf-core/chipseq/bin/ def consensus_dir = "${aligner_dir}/mergedLibrary/macs2/${peak_dir}/consensus/*" """ diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index 31552551..d23d2cbe 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -56,6 +56,9 @@ process MULTIQC { path "*_plots" , optional:true, emit: plots path "versions.yml" , emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = task.ext.args ?: '' def custom_config = params.multiqc_config ? "--config $mqc_custom_config" : '' diff --git a/modules/local/multiqc_custom_peaks.nf b/modules/local/multiqc_custom_peaks.nf index 8fabe949..c19d81a5 100644 --- a/modules/local/multiqc_custom_peaks.nf +++ b/modules/local/multiqc_custom_peaks.nf @@ -14,6 +14,9 @@ process MULTIQC_CUSTOM_PEAKS { tuple val(meta), path("*.peak_count_mqc.tsv"), emit: count tuple val(meta), path("*.FRiP_mqc.tsv") , emit: frip + when: + task.ext.when == null || task.ext.when + script: def prefix = task.ext.prefix ?: "${meta.id}" """ diff --git a/modules/local/multiqc_custom_phantompeakqualtools.nf b/modules/local/multiqc_custom_phantompeakqualtools.nf index c7f4127f..9e59d057 100644 --- a/modules/local/multiqc_custom_phantompeakqualtools.nf +++ b/modules/local/multiqc_custom_phantompeakqualtools.nf @@ -16,6 +16,9 @@ process MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS { tuple val(meta), path("*.spp_rsc_mqc.tsv") , emit: rsc tuple val(meta), path("*.spp_correlation_mqc.tsv"), emit: correlation + when: + task.ext.when == null || task.ext.when + script: def prefix = task.ext.prefix ?: "${meta.id}" """ diff --git a/modules/local/plot_homer_annotatepeaks.nf b/modules/local/plot_homer_annotatepeaks.nf index eed17949..36c4730e 100644 --- a/modules/local/plot_homer_annotatepeaks.nf +++ b/modules/local/plot_homer_annotatepeaks.nf @@ -17,6 +17,9 @@ process PLOT_HOMER_ANNOTATEPEAKS { path '*.tsv' , emit: tsv path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "annotatepeaks" diff --git a/modules/local/plot_macs2_qc.nf b/modules/local/plot_macs2_qc.nf index 2915b739..7599c9f8 100644 --- a/modules/local/plot_macs2_qc.nf +++ b/modules/local/plot_macs2_qc.nf @@ -15,6 +15,9 @@ process PLOT_MACS2_QC { path '*.pdf' , emit: pdf path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ def args = task.ext.args ?: '' def peak_type = is_narrow_peak ? 'narrowPeak' : 'broadPeak' diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index c28658f5..583c436f 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -21,7 +21,7 @@ process SAMPLESHEET_CHECK { """ check_samplesheet.py \\ $samplesheet \\ - samplesheet.valid.csv + $args cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/local/star_genomegenerate.nf b/modules/local/star_genomegenerate.nf index 904b0714..285df97c 100644 --- a/modules/local/star_genomegenerate.nf +++ b/modules/local/star_genomegenerate.nf @@ -16,6 +16,9 @@ process STAR_GENOMEGENERATE { path "star" , emit: index path "versions.yml", emit: versions + when: + task.ext.when == null || task.ext.when + script: def args = (task.ext.args ?: '').tokenize() def memory = task.memory ? "--limitGenomeGenerateRAM ${task.memory.toBytes() - 100000000}" : '' From eca2e06718df27dd1f7a74db5bf04fa9172f5c0c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 26 Jun 2023 18:58:30 +0200 Subject: [PATCH 102/201] Fix typo --- modules/local/bedtools_genomecov.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/bedtools_genomecov.nf b/modules/local/bedtools_genomecov.nf index eb3d0dcc..28dcc5dd 100644 --- a/modules/local/bedtools_genomecov.nf +++ b/modules/local/bedtools_genomecov.nf @@ -32,7 +32,7 @@ process BEDTOOLS_GENOMECOV { -bg \\ -scale \$SCALE_FACTOR \\ $pe \\ - $extend \\ + $args \\ | sort -T '.' -k1,1 -k2,2n > ${prefix}.bedGraph cat <<-END_VERSIONS > versions.yml From 4aa413dec02fdbf3509ba90c38b7982bd3eb1e86 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 26 Jun 2023 19:36:31 +0200 Subject: [PATCH 103/201] Read args from samplesheet_check --- modules/local/samplesheet_check.nf | 1 + 1 file changed, 1 insertion(+) diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf index 583c436f..216e9fb7 100644 --- a/modules/local/samplesheet_check.nf +++ b/modules/local/samplesheet_check.nf @@ -18,6 +18,7 @@ process SAMPLESHEET_CHECK { task.ext.when == null || task.ext.when script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ + def args = task.ext.args ?: '' """ check_samplesheet.py \\ $samplesheet \\ From 4ab68b3201245fdf0f0df11bad556aa794cb9852 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 26 Jun 2023 23:15:09 +0200 Subject: [PATCH 104/201] Update output --- conf/modules.config | 4 ++-- docs/output.md | 30 +++++++++++++++--------------- 2 files changed, 17 insertions(+), 17 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index a112e27d..adb500b3 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -67,7 +67,7 @@ process { ] } - withName: 'BWA_INDEX|BOWTIE2_BUILD|STAR_GENOMEGENERATE|CHROMAP_INDEX' { + withName: 'BWA_INDEX|BOWTIE2_BUILD|STAR_GENOMEGENERATE' { publishDir = [ path: { "${params.outdir}/genome/index" }, mode: params.publish_dir_mode, @@ -257,7 +257,7 @@ if (params.aligner == 'chromap') { process { withName: 'CHROMAP_INDEX' { publishDir = [ - path: { "${params.outdir}/genome/${params.aligner}/index" }, + path: { "${params.outdir}/genome/index/${params.aligner}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/docs/output.md b/docs/output.md index b3198554..065dcfd3 100644 --- a/docs/output.md +++ b/docs/output.md @@ -108,19 +108,19 @@ The library-level alignments associated with the same sample are merged and subs
    Output files -- `/mergedLibrary/` +- `/merged_library/` - `*.bam`: Merged library-level, coordinate sorted `*.bam` files after the marking of duplicates, and filtering based on various criteria. The file suffix for the final filtered files will be `*.mLb.clN.*`. If you specify the `--save_align_intermeds` parameter then two additional sets of files will be present. These represent the unfiltered alignments with duplicates marked (`*.mLb.mkD.*`), and in the case of paired-end datasets the filtered alignments before the removal of orphan read pairs (`*.mLb.flT.*`). -- `/mergedLibrary/samtools_stats/` +- `/merged_library/samtools_stats/` - SAMtools `*.flagstat`, `*.idxstats` and `*.stats` files generated from the alignment files. -- `/mergedLibrary/picard_metrics/` +- `/merged_library/picard_metrics/` - `*_metrics`: Alignment QC files from picard CollectMultipleMetrics. - `*.metrics.txt`: Metrics file from MarkDuplicates. -- `/mergedLibrary/picard_metrics/pdf/` +- `/merged_library/picard_metrics/pdf/` - `*.pdf`: Alignment QC plot files from picard CollectMultipleMetrics. -- `/mergedLibrary/preseq/` +- `/merged_library/preseq/` - `*.lc_extrap.txt`: Preseq expected future yield file. -> **NB:** File names in the resulting directory (i.e. `/mergedLibrary/`) will have the '`.mLb.`' suffix. +> **NB:** File names in the resulting directory (i.e. `/merged_library/`) will have the '`.mLb.`' suffix.
    @@ -141,7 +141,7 @@ The [Preseq](http://smithlabresearch.org/software/preseq/) package is aimed at p
    Output files -- `/mergedLibrary/bigwig/` +- `/merged_library/bigwig/` - `*.bigWig`: Normalised bigWig files scaled to 1 million mapped reads.
    @@ -153,12 +153,12 @@ The [bigWig](https://genome.ucsc.edu/goldenpath/help/bigWig.html) format is in a
    Output files -- `/mergedLibrary/phantompeakqualtools/` +- `/merged_library/phantompeakqualtools/` - `*.spp.out`, `*.spp.pdf`: phantompeakqualtools output files. - `*_mqc.tsv`: MultiQC custom content files. -- `/mergedLibrary/deepTools/plotFingerprint/` +- `/merged_library/deepTools/plotFingerprint/` - `*.plotFingerprint.pdf`, `*.plotFingerprint.qcmetrics.txt`, `*.plotFingerprint.raw.txt`: plotFingerprint output files. -- `/mergedLibrary/deepTools/plotProfile/` +- `/merged_library/deepTools/plotProfile/` - `*.computeMatrix.mat.gz`, `*.computeMatrix.vals.mat.tab`, `*.plotProfile.pdf`, `*.plotProfile.tab`, `*.plotHeatmap.pdf`, `*.plotHeatmap.mat.tab`: plotProfile output files.
    @@ -188,10 +188,10 @@ The results from deepTools plotProfile gives you a quick visualisation for the g
    Output files -- `/mergedLibrary/macs2//` +- `/merged_library/macs2//` - `*.xls`, `*.broadPeak` or `*.narrowPeak`, `*.gappedPeak`, `*summits.bed`: MACS2 output files - the files generated will depend on whether MACS2 has been run in _narrowPeak_ or _broadPeak_ mode. - `*.annotatePeaks.txt`: HOMER peak-to-gene annotation file. -- `/mergedLibrary/macs2//qc/` +- `/merged_library/macs2//qc/` - `macs2_peak.plots.pdf`: QC plots for MACS2 peaks. - `macs2_annotatePeaks.plots.pdf`: QC plots for peak-to-gene feature annotation. - `*.FRiP_mqc.tsv`, `*.peak_count_mqc.tsv`, `annotatepeaks.summary_mqc.tsv`: MultiQC custom-content files for FRiP score, peak count and peak-to-gene ratios. @@ -217,7 +217,7 @@ Various QC plots per sample including number of peaks, fold-change distribution,
    Output files -- `/mergedLibrary/macs2//consensus//` +- `/merged_library/macs2//consensus//` - `*.bed`: Consensus peak-set across all samples in BED format. - `*.saf`: Consensus peak-set across all samples in SAF format. Required by featureCounts for read quantification. - `*.featureCounts.txt`: Read counts across all samples relative to consensus peak-set. @@ -245,7 +245,7 @@ The [featureCounts](http://bioinf.wehi.edu.au/featureCounts/) tool is used to co
    Output files -- `/mergedLibrary/macs2//consensus//deseq2/` +- `/merged_library/macs2//consensus//deseq2/` - `*.sample.dists.txt`: Spreadsheet containing sample-to-sample distance across each consensus peak. - `*.plots.pdf`: File containing PCA and hierarchical clustering plots. - `*.dds.RData`: File containing R `DESeqDataSet` object generated by DESeq2, with either @@ -254,7 +254,7 @@ The [featureCounts](http://bioinf.wehi.edu.au/featureCounts/) tool is used to co `readRDS` to give user control of the eventual object name. - `*pca.vals.txt`: Matrix of values for the first 2 principal components. - `R_sessionInfo.log`: File containing information about R, the OS and attached or loaded packages. - - `/mergedLibrary/macs2//consensus//sizeFactors/` + - `/merged_library/macs2//consensus//sizeFactors/` - `*.txt`, `*.RData`: Files containing DESeq2 sizeFactors per sample.
    From 5d8b57061a572c54bce9e1ca8da4006cab21598a Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 27 Jun 2023 19:41:59 +0200 Subject: [PATCH 105/201] Apply review changes --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index adb500b3..454f6dfa 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -456,7 +456,7 @@ process { } withName: 'UCSC_BEDGRAPHTOBIGWIG' { - ext.prefix = { "${meta.id}..mLb.clN" } + ext.prefix = { "${meta.id}.mLb.clN" } publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/bigwig" }, mode: params.publish_dir_mode, From eb7c34941880971aad7ce9c5e994afa1a1577d56 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 27 Jun 2023 22:41:49 +0200 Subject: [PATCH 106/201] Apply review changes --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 454f6dfa..ffcbb614 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -438,7 +438,7 @@ process { ext.args = { [ (meta.single_end && params.fragment_size > 0) ? "-fs ${params.fragment_size}" : '' - ].join(' ').trim() + ].trim() } ext.prefix = { "${meta.id}.mLB.clN" } publishDir = [ From 0ae5d55703cb931a2d02cb539683c2e31b49b943 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 27 Jun 2023 23:19:57 +0200 Subject: [PATCH 107/201] Revert change --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index ffcbb614..454f6dfa 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -438,7 +438,7 @@ process { ext.args = { [ (meta.single_end && params.fragment_size > 0) ? "-fs ${params.fragment_size}" : '' - ].trim() + ].join(' ').trim() } ext.prefix = { "${meta.id}.mLB.clN" } publishDir = [ From 2a5f3f4e5aa4129712dc1a5fa1979f1eddfb0977 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 28 Jun 2023 12:57:48 +0200 Subject: [PATCH 108/201] Get rid of unused list for ext.args --- conf/modules.config | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 454f6dfa..6b67b9aa 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -435,11 +435,7 @@ process { } withName: 'BEDTOOLS_GENOMECOV' { - ext.args = { - [ - (meta.single_end && params.fragment_size > 0) ? "-fs ${params.fragment_size}" : '' - ].join(' ').trim() - } + ext.args = { (meta.single_end && params.fragment_size > 0) ? "-fs ${params.fragment_size}" : '' } ext.prefix = { "${meta.id}.mLB.clN" } publishDir = [ [ From e437a7c8b33a03dd0629038a51912740254059da Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Fri, 30 Jun 2023 16:13:31 +0000 Subject: [PATCH 109/201] Template update for nf-core/tools version 2.9 --- .github/CONTRIBUTING.md | 1 - .github/ISSUE_TEMPLATE/bug_report.yml | 2 +- .github/workflows/awsfulltest.yml | 11 +- .github/workflows/awstest.yml | 10 +- .github/workflows/ci.yml | 2 +- .gitpod.yml | 5 + CITATIONS.md | 6 + README.md | 6 +- assets/methods_description_template.yml | 12 +- assets/multiqc_config.yml | 4 +- assets/nf-core-chipseq_logo_light.png | Bin 10959 -> 73788 bytes assets/slackreport.json | 2 +- conf/test_full.config | 2 - docs/usage.md | 6 +- lib/NfcoreSchema.groovy | 530 ------------------------ lib/NfcoreTemplate.groovy | 2 +- lib/WorkflowChipseq.groovy | 45 +- lib/WorkflowMain.groovy | 37 -- main.nf | 16 + nextflow.config | 52 ++- nextflow_schema.json | 36 +- workflows/chipseq.nf | 25 +- 22 files changed, 175 insertions(+), 637 deletions(-) delete mode 100755 lib/NfcoreSchema.groovy diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 5d23c1d9..8c24b7f1 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -116,4 +116,3 @@ To get started: Devcontainer specs: - [DevContainer config](.devcontainer/devcontainer.json) -- [Dockerfile](.devcontainer/Dockerfile) diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml index d71ef79d..e2bb24d5 100644 --- a/.github/ISSUE_TEMPLATE/bug_report.yml +++ b/.github/ISSUE_TEMPLATE/bug_report.yml @@ -42,7 +42,7 @@ body: attributes: label: System information description: | - * Nextflow version _(eg. 22.10.1)_ + * Nextflow version _(eg. 23.04.0)_ * Hardware _(eg. HPC, Desktop, Cloud)_ * Executor _(eg. slurm, local, awsbatch)_ * Container engine: _(e.g. Docker, Singularity, Conda, Podman, Shifter, Charliecloud, or Apptainer)_ diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 31ac32b9..500a2167 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v1 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -22,13 +22,18 @@ jobs: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} + revision: ${{ github.sha }} workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/chipseq/work-${{ github.sha }} parameters: | { + "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}", "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/chipseq/results-${{ github.sha }}" } - profiles: test_full,aws_tower + profiles: test_full + - uses: actions/upload-artifact@v3 with: name: Tower debug log file - path: tower_action_*.log + path: | + tower_action_*.log + tower_action_*.json diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 153490d0..6b16318d 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,18 +12,22 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v1 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} compute_env: ${{ secrets.TOWER_COMPUTE_ENV }} + revision: ${{ github.sha }} workdir: s3://${{ secrets.AWS_S3_BUCKET }}/work/chipseq/work-${{ github.sha }} parameters: | { "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/chipseq/results-test-${{ github.sha }}" } - profiles: test,aws_tower + profiles: test + - uses: actions/upload-artifact@v3 with: name: Tower debug log file - path: tower_action_*.log + path: | + tower_action_*.log + tower_action_*.json diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index fb742746..092835bc 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ jobs: strategy: matrix: NXF_VER: - - "22.10.1" + - "23.04.0" - "latest-everything" steps: - name: Check out pipeline code diff --git a/.gitpod.yml b/.gitpod.yml index 85d95ecc..25488dcc 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -1,4 +1,9 @@ image: nfcore/gitpod:latest +tasks: + - name: Update Nextflow and setup pre-commit + command: | + pre-commit install --install-hooks + nextflow self-update vscode: extensions: # based on nf-core.nf-core-extensionpack diff --git a/CITATIONS.md b/CITATIONS.md index b4b3c896..d47e0566 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,7 +12,10 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) + > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. Available online https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. + - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) + > Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924. ## Software packaging/containerisation tools @@ -31,5 +34,8 @@ - [Docker](https://dl.acm.org/doi/10.5555/2600239.2600241) + > Merkel, D. (2014). Docker: lightweight linux containers for consistent development and deployment. Linux Journal, 2014(239), 2. doi: 10.5555/2600239.2600241. + - [Singularity](https://pubmed.ncbi.nlm.nih.gov/28494014/) + > Kurtzer GM, Sochat V, Bauer MW. Singularity: Scientific containers for mobility of compute. PLoS One. 2017 May 11;12(5):e0177459. doi: 10.1371/journal.pone.0177459. eCollection 2017. PubMed PMID: 28494014; PubMed Central PMCID: PMC5426675. diff --git a/README.md b/README.md index 9a7611f6..e5953300 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ [![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) -[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A522.10.1-23aa62.svg)](https://www.nextflow.io/) +[![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) @@ -66,11 +66,11 @@ nextflow run nf-core/chipseq \ > provided by the `-c` Nextflow option can be used to provide any configuration _**except for parameters**_; > see [docs](https://nf-co.re/usage/configuration#custom-configuration-files). -For more details, please refer to the [usage documentation](https://nf-co.re/chipseq/usage) and the [parameter documentation](https://nf-co.re/chipseq/parameters). +For more details and further functionality, please refer to the [usage documentation](https://nf-co.re/chipseq/usage) and the [parameter documentation](https://nf-co.re/chipseq/parameters). ## Pipeline output -To see the the results of a test run with a full size dataset refer to the [results](https://nf-co.re/chipseq/results) tab on the nf-core website pipeline page. +To see the results of an example test run with a full size dataset refer to the [results](https://nf-co.re/chipseq/results) tab on the nf-core website pipeline page. For more details about the output files and reports, please refer to the [output documentation](https://nf-co.re/chipseq/output). diff --git a/assets/methods_description_template.yml b/assets/methods_description_template.yml index a30b1eb6..4ff6034f 100644 --- a/assets/methods_description_template.yml +++ b/assets/methods_description_template.yml @@ -3,17 +3,21 @@ description: "Suggested text and references to use when describing pipeline usag section_name: "nf-core/chipseq Methods Description" section_href: "https://github.com/nf-core/chipseq" plot_type: "html" -## TODO nf-core: Update the HTML below to your prefered methods description, e.g. add publication citation for this pipeline +## TODO nf-core: Update the HTML below to your preferred methods description, e.g. add publication citation for this pipeline ## You inject any metadata in the Nextflow '${workflow}' object data: |

    Methods

    -

    Data was processed using nf-core/chipseq v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020).

    +

    Data was processed using nf-core/chipseq v${workflow.manifest.version} ${doi_text} of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

    The pipeline was executed with Nextflow v${workflow.nextflow.version} (Di Tommaso et al., 2017) with the following command:

    ${workflow.commandLine}
    +

    ${tool_citations}

    References

      -
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. https://doi.org/10.1038/nbt.3820
      • -
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. https://doi.org/10.1038/s41587-020-0439-x
      • +
    • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
      • +
    • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
      • +
    • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
      • +
    • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
      • + ${tool_bibliography}
    Notes:
    diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 46d7ec2d..548b7c12 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/chipseq + This report has been generated by the nf-core/chipseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-chipseq-methods-description": order: -1000 diff --git a/assets/nf-core-chipseq_logo_light.png b/assets/nf-core-chipseq_logo_light.png index 21300d1d37f585ca022911966f6413c2895c66b2..51a83b3d789d5852acc2bb044650b13785c6bf41 100644 GIT binary patch literal 73788 zcmeEt`9IX_`~RRQS?ZKSWhn*~p=96c5GGq9OZHNfecuPCQz(&w!1jG0qM))u18{N;szxKLnntC7*Z0~7*=;B1!jv^4p5Gb_^hQ29NgTYTSd@O|5 zS3HI44fR<@BwC_WweNAg^K`t?ay|Ua^`zuS;o*5X;p5j0nLR_3TdTw-*C$<<{Vk$; z9`%au>-b1%=CCl=x~!Jp!Br{RFpzjKp!3X+Tb;*QRKss@Kb){h^c+@seV?p-3zMBT zv9)Zlu({<`v3Pc z_~QTk@G~L)&kz6ShyTBGp!b^mFYH1%8g&}PE+NMRdy{Rgwkaa9QvrRQY2HJz)6`6H z9;J$!8p?T$p0J;N*Ye!J#ykH8M)iUCxVX5E!@pK|Rzc1t45Gxe-2E^GvsRWhY(8G+ zqQw!LH!;zIl^)J$8$X^IcCItbD!;xEnF(K*M&+X@JSfW~(%%?AjAD}I{FvT)!b;+< zT`3RVvHyDV#tr{F?pFSzX|tN{P8k1QHN6RI-9sVD@-lUEm%l0Eg`Uqb{CpIznVgoC zqUmmd=@Irb{U+;BnnF@S4JpEd=f8=bxA|}L4A?vsm9JMY?xEj%PSrz{(B9T6zCrD{ 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} else { %>danger<%} %>", - "author_name": "sanger-tol/readmapping v${version} - ${runName}", + "author_name": "nf-core/chipseq v${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/test_full.config b/conf/test_full.config index 1505bc42..5c3bc080 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -10,8 +10,6 @@ ---------------------------------------------------------------------------------------- */ -cleanup = true - params { config_profile_name = 'Full test profile' config_profile_description = 'Full test dataset to check pipeline function' diff --git a/docs/usage.md b/docs/usage.md index 9f415ef9..13aaf242 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -57,7 +57,7 @@ An [example samplesheet](../assets/samplesheet.csv) has been provided with the p The typical command for running the pipeline is as follows: ```bash -nextflow run nf-core/chipseq --input samplesheet.csv --outdir --genome GRCh37 -profile docker +nextflow run nf-core/chipseq --input ./samplesheet.csv --outdir ./results --genome GRCh37 -profile docker ``` This will launch the pipeline with the `docker` configuration profile. See below for more information about profiles. @@ -76,7 +76,8 @@ If you wish to repeatedly use the same parameters for multiple runs, rather than Pipeline settings can be provided in a `yaml` or `json` file via `-params-file `. > ⚠️ Do not use `-c ` to specify parameters as this will result in errors. Custom config files specified with `-c` must only be used for [tuning process resource specifications](https://nf-co.re/docs/usage/configuration#tuning-workflow-resources), other infrastructural tweaks (such as output directories), or module arguments (args). -> The above pipeline run specified with a params file in yaml format: + +The above pipeline run specified with a params file in yaml format: ```bash nextflow run nf-core/chipseq -profile docker -params-file params.yaml @@ -88,7 +89,6 @@ with `params.yaml` containing: input: './samplesheet.csv' outdir: './results/' genome: 'GRCh37' -input: 'data' <...> ``` diff --git a/lib/NfcoreSchema.groovy b/lib/NfcoreSchema.groovy deleted file mode 100755 index 9b34804d..00000000 --- a/lib/NfcoreSchema.groovy +++ /dev/null @@ -1,530 +0,0 @@ -// -// This file holds several functions used to perform JSON parameter validation, help and summary rendering for the nf-core pipeline template. -// - -import nextflow.Nextflow -import org.everit.json.schema.Schema -import org.everit.json.schema.loader.SchemaLoader -import org.everit.json.schema.ValidationException -import org.json.JSONObject -import org.json.JSONTokener -import org.json.JSONArray -import groovy.json.JsonSlurper -import groovy.json.JsonBuilder - -class NfcoreSchema { - - // - // Resolve Schema path relative to main workflow directory - // - public static String getSchemaPath(workflow, schema_filename='nextflow_schema.json') { - return "${workflow.projectDir}/${schema_filename}" - } - - // - // Function to loop over all parameters defined in schema and check - // whether the given parameters adhere to the specifications - // - /* groovylint-disable-next-line UnusedPrivateMethodParameter */ - public static void validateParameters(workflow, params, log, schema_filename='nextflow_schema.json') { - def has_error = false - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Check for nextflow core params and unexpected params - def json = new File(getSchemaPath(workflow, schema_filename=schema_filename)).text - def Map schemaParams = (Map) new JsonSlurper().parseText(json).get('definitions') - def nf_params = [ - // Options for base `nextflow` command - 'bg', - 'c', - 'C', - 'config', - 'd', - 'D', - 'dockerize', - 'h', - 'log', - 'q', - 'quiet', - 'syslog', - 'v', - - // Options for `nextflow run` command - 'ansi', - 'ansi-log', - 'bg', - 'bucket-dir', - 'c', - 'cache', - 'config', - 'dsl2', - 'dump-channels', - 'dump-hashes', - 'E', - 'entry', - 'latest', - 'lib', - 'main-script', - 'N', - 'name', - 'offline', - 'params-file', - 'pi', - 'plugins', - 'poll-interval', - 'pool-size', - 'profile', - 'ps', - 'qs', - 'queue-size', - 'r', - 'resume', - 'revision', - 'stdin', - 'stub', - 'stub-run', - 'test', - 'w', - 'with-apptainer', - 'with-charliecloud', - 'with-conda', - 'with-dag', - 'with-docker', - 'with-mpi', - 'with-notification', - 'with-podman', - 'with-report', - 'with-singularity', - 'with-timeline', - 'with-tower', - 'with-trace', - 'with-weblog', - 'without-docker', - 'without-podman', - 'work-dir' - ] - def unexpectedParams = [] - - // Collect expected parameters from the schema - def expectedParams = [] - def enums = [:] - for (group in schemaParams) { - for (p in group.value['properties']) { - expectedParams.push(p.key) - if (group.value['properties'][p.key].containsKey('enum')) { - enums[p.key] = group.value['properties'][p.key]['enum'] - } - } - } - - for (specifiedParam in params.keySet()) { - // nextflow params - if (nf_params.contains(specifiedParam)) { - log.error "ERROR: You used a core Nextflow option with two hyphens: '--${specifiedParam}'. Please resubmit with '-${specifiedParam}'" - has_error = true - } - // unexpected params - def params_ignore = params.schema_ignore_params.split(',') + 'schema_ignore_params' - def expectedParamsLowerCase = expectedParams.collect{ it.replace("-", "").toLowerCase() } - def specifiedParamLowerCase = specifiedParam.replace("-", "").toLowerCase() - def isCamelCaseBug = (specifiedParam.contains("-") && !expectedParams.contains(specifiedParam) && expectedParamsLowerCase.contains(specifiedParamLowerCase)) - if (!expectedParams.contains(specifiedParam) && !params_ignore.contains(specifiedParam) && !isCamelCaseBug) { - // Temporarily remove camelCase/camel-case params #1035 - def unexpectedParamsLowerCase = unexpectedParams.collect{ it.replace("-", "").toLowerCase()} - if (!unexpectedParamsLowerCase.contains(specifiedParamLowerCase)){ - unexpectedParams.push(specifiedParam) - } - } - } - - //~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~// - // Validate parameters against the schema - InputStream input_stream = new File(getSchemaPath(workflow, schema_filename=schema_filename)).newInputStream() - JSONObject raw_schema = new JSONObject(new JSONTokener(input_stream)) - - // Remove anything that's in params.schema_ignore_params - raw_schema = removeIgnoredParams(raw_schema, params) - - Schema schema = SchemaLoader.load(raw_schema) - - // Clean the parameters - def cleanedParams = cleanParameters(params) - - // Convert to JSONObject - def jsonParams = new JsonBuilder(cleanedParams) - JSONObject params_json = new JSONObject(jsonParams.toString()) - - // Validate - try { - schema.validate(params_json) - } catch (ValidationException e) { - println '' - log.error 'ERROR: Validation of pipeline parameters failed!' - JSONObject exceptionJSON = e.toJSON() - printExceptions(exceptionJSON, params_json, log, enums) - println '' - has_error = true - } - - // Check for unexpected parameters - if (unexpectedParams.size() > 0) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - println '' - def warn_msg = 'Found unexpected parameters:' - for (unexpectedParam in unexpectedParams) { - warn_msg = warn_msg + "\n* --${unexpectedParam}: ${params[unexpectedParam].toString()}" - } - log.warn warn_msg - log.info "- ${colors.dim}Ignore this warning: params.schema_ignore_params = \"${unexpectedParams.join(',')}\" ${colors.reset}" - println '' - } - - if (has_error) { - Nextflow.error('Exiting!') - } - } - - // - // Beautify parameters for --help - // - public static String paramsHelp(workflow, params, command, schema_filename='nextflow_schema.json') { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - Integer num_hidden = 0 - String output = '' - output += 'Typical pipeline command:\n\n' - output += " ${colors.cyan}${command}${colors.reset}\n\n" - Map params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - Integer max_chars = paramsMaxChars(params_map) + 1 - Integer desc_indent = max_chars + 14 - Integer dec_linewidth = 160 - desc_indent - for (group in params_map.keySet()) { - Integer num_params = 0 - String group_output = colors.underlined + colors.bold + group + colors.reset + '\n' - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (group_params.get(param).hidden && !params.show_hidden_params) { - num_hidden += 1 - continue; - } - def type = '[' + group_params.get(param).type + ']' - def description = group_params.get(param).description - def defaultValue = group_params.get(param).default != null ? " [default: " + group_params.get(param).default.toString() + "]" : '' - def description_default = description + colors.dim + defaultValue + colors.reset - // Wrap long description texts - // Loosely based on https://dzone.com/articles/groovy-plain-text-word-wrap - if (description_default.length() > dec_linewidth){ - List olines = [] - String oline = "" // " " * indent - description_default.split(" ").each() { wrd -> - if ((oline.size() + wrd.size()) <= dec_linewidth) { - oline += wrd + " " - } else { - olines += oline - oline = wrd + " " - } - } - olines += oline - description_default = olines.join("\n" + " " * desc_indent) - } - group_output += " --" + param.padRight(max_chars) + colors.dim + type.padRight(10) + colors.reset + description_default + '\n' - num_params += 1 - } - group_output += '\n' - if (num_params > 0){ - output += group_output - } - } - if (num_hidden > 0){ - output += colors.dim + "!! Hiding $num_hidden params, use --show_hidden_params to show them !!\n" + colors.reset - } - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Groovy Map summarising parameters/workflow options used by the pipeline - // - public static LinkedHashMap paramsSummaryMap(workflow, params, schema_filename='nextflow_schema.json') { - // Get a selection of core Nextflow workflow options - def Map workflow_summary = [:] - if (workflow.revision) { - workflow_summary['revision'] = workflow.revision - } - workflow_summary['runName'] = workflow.runName - if (workflow.containerEngine) { - workflow_summary['containerEngine'] = workflow.containerEngine - } - if (workflow.container) { - workflow_summary['container'] = workflow.container - } - workflow_summary['launchDir'] = workflow.launchDir - workflow_summary['workDir'] = workflow.workDir - workflow_summary['projectDir'] = workflow.projectDir - workflow_summary['userName'] = workflow.userName - workflow_summary['profile'] = workflow.profile - workflow_summary['configFiles'] = workflow.configFiles.join(', ') - - // Get pipeline parameters defined in JSON Schema - def Map params_summary = [:] - def params_map = paramsLoad(getSchemaPath(workflow, schema_filename=schema_filename)) - for (group in params_map.keySet()) { - def sub_params = new LinkedHashMap() - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (params.containsKey(param)) { - def params_value = params.get(param) - def schema_value = group_params.get(param).default - def param_type = group_params.get(param).type - if (schema_value != null) { - if (param_type == 'string') { - if (schema_value.contains('$projectDir') || schema_value.contains('${projectDir}')) { - def sub_string = schema_value.replace('\$projectDir', '') - sub_string = sub_string.replace('\${projectDir}', '') - if (params_value.contains(sub_string)) { - schema_value = params_value - } - } - if (schema_value.contains('$params.outdir') || schema_value.contains('${params.outdir}')) { - def sub_string = schema_value.replace('\$params.outdir', '') - sub_string = sub_string.replace('\${params.outdir}', '') - if ("${params.outdir}${sub_string}" == params_value) { - schema_value = params_value - } - } - } - } - - // We have a default in the schema, and this isn't it - if (schema_value != null && params_value != schema_value) { - sub_params.put(param, params_value) - } - // No default in the schema, and this isn't empty - else if (schema_value == null && params_value != "" && params_value != null && params_value != false) { - sub_params.put(param, params_value) - } - } - } - params_summary.put(group, sub_params) - } - return [ 'Core Nextflow options' : workflow_summary ] << params_summary - } - - // - // Beautify parameters for summary and return as string - // - public static String paramsSummaryLog(workflow, params) { - Map colors = NfcoreTemplate.logColours(params.monochrome_logs) - String output = '' - def params_map = paramsSummaryMap(workflow, params) - def max_chars = paramsMaxChars(params_map) - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - if (group_params) { - output += colors.bold + group + colors.reset + '\n' - for (param in group_params.keySet()) { - output += " " + colors.blue + param.padRight(max_chars) + ": " + colors.green + group_params.get(param) + colors.reset + '\n' - } - output += '\n' - } - } - output += "!! Only displaying parameters that differ from the pipeline defaults !!\n" - output += NfcoreTemplate.dashedLine(params.monochrome_logs) - return output - } - - // - // Loop over nested exceptions and print the causingException - // - private static void printExceptions(ex_json, params_json, log, enums, limit=5) { - def causingExceptions = ex_json['causingExceptions'] - if (causingExceptions.length() == 0) { - def m = ex_json['message'] =~ /required key \[([^\]]+)\] not found/ - // Missing required param - if (m.matches()) { - log.error "* Missing required parameter: --${m[0][1]}" - } - // Other base-level error - else if (ex_json['pointerToViolation'] == '#') { - log.error "* ${ex_json['message']}" - } - // Error with specific param - else { - def param = ex_json['pointerToViolation'] - ~/^#\// - def param_val = params_json[param].toString() - if (enums.containsKey(param)) { - def error_msg = "* --${param}: '${param_val}' is not a valid choice (Available choices" - if (enums[param].size() > limit) { - log.error "${error_msg} (${limit} of ${enums[param].size()}): ${enums[param][0..limit-1].join(', ')}, ... )" - } else { - log.error "${error_msg}: ${enums[param].join(', ')})" - } - } else { - log.error "* --${param}: ${ex_json['message']} (${param_val})" - } - } - } - for (ex in causingExceptions) { - printExceptions(ex, params_json, log, enums) - } - } - - // - // Remove an element from a JSONArray - // - private static JSONArray removeElement(json_array, element) { - def list = [] - int len = json_array.length() - for (int i=0;i - if(raw_schema.keySet().contains('definitions')){ - raw_schema.definitions.each { definition -> - for (key in definition.keySet()){ - if (definition[key].get("properties").keySet().contains(ignore_param)){ - // Remove the param to ignore - definition[key].get("properties").remove(ignore_param) - // If the param was required, change this - if (definition[key].has("required")) { - def cleaned_required = removeElement(definition[key].required, ignore_param) - definition[key].put("required", cleaned_required) - } - } - } - } - } - if(raw_schema.keySet().contains('properties') && raw_schema.get('properties').keySet().contains(ignore_param)) { - raw_schema.get("properties").remove(ignore_param) - } - if(raw_schema.keySet().contains('required') && raw_schema.required.contains(ignore_param)) { - def cleaned_required = removeElement(raw_schema.required, ignore_param) - raw_schema.put("required", cleaned_required) - } - } - return raw_schema - } - - // - // Clean and check parameters relative to Nextflow native classes - // - private static Map cleanParameters(params) { - def new_params = params.getClass().newInstance(params) - for (p in params) { - // remove anything evaluating to false - if (!p['value']) { - new_params.remove(p.key) - } - // Cast MemoryUnit to String - if (p['value'].getClass() == nextflow.util.MemoryUnit) { - new_params.replace(p.key, p['value'].toString()) - } - // Cast Duration to String - if (p['value'].getClass() == nextflow.util.Duration) { - new_params.replace(p.key, p['value'].toString().replaceFirst(/d(?!\S)/, "day")) - } - // Cast LinkedHashMap to String - if (p['value'].getClass() == LinkedHashMap) { - new_params.replace(p.key, p['value'].toString()) - } - } - return new_params - } - - // - // This function tries to read a JSON params file - // - private static LinkedHashMap paramsLoad(String json_schema) { - def params_map = new LinkedHashMap() - try { - params_map = paramsRead(json_schema) - } catch (Exception e) { - println "Could not read parameters settings from JSON. $e" - params_map = new LinkedHashMap() - } - return params_map - } - - // - // Method to actually read in JSON file using Groovy. - // Group (as Key), values are all parameters - // - Parameter1 as Key, Description as Value - // - Parameter2 as Key, Description as Value - // .... - // Group - // - - private static LinkedHashMap paramsRead(String json_schema) throws Exception { - def json = new File(json_schema).text - def Map schema_definitions = (Map) new JsonSlurper().parseText(json).get('definitions') - def Map schema_properties = (Map) new JsonSlurper().parseText(json).get('properties') - /* Tree looks like this in nf-core schema - * definitions <- this is what the first get('definitions') gets us - group 1 - title - description - properties - parameter 1 - type - description - parameter 2 - type - description - group 2 - title - description - properties - parameter 1 - type - description - * properties <- parameters can also be ungrouped, outside of definitions - parameter 1 - type - description - */ - - // Grouped params - def params_map = new LinkedHashMap() - schema_definitions.each { key, val -> - def Map group = schema_definitions."$key".properties // Gets the property object of the group - def title = schema_definitions."$key".title - def sub_params = new LinkedHashMap() - group.each { innerkey, value -> - sub_params.put(innerkey, value) - } - params_map.put(title, sub_params) - } - - // Ungrouped params - def ungrouped_params = new LinkedHashMap() - schema_properties.each { innerkey, value -> - ungrouped_params.put(innerkey, value) - } - params_map.put("Other parameters", ungrouped_params) - - return params_map - } - - // - // Get maximum number of characters across all parameter names - // - private static Integer paramsMaxChars(params_map) { - Integer max_chars = 0 - for (group in params_map.keySet()) { - def group_params = params_map.get(group) // This gets the parameters of that particular group - for (param in group_params.keySet()) { - if (param.size() > max_chars) { - max_chars = param.size() - } - } - } - return max_chars - } -} diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 25a0a74a..408951ae 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -128,7 +128,7 @@ class NfcoreTemplate { def email_html = html_template.toString() // Render the sendmail template - def max_multiqc_email_size = params.max_multiqc_email_size as nextflow.util.MemoryUnit + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] def sf = new File("$projectDir/assets/sendmail_template.txt") def sendmail_template = engine.createTemplate(sf).make(smail_fields) diff --git a/lib/WorkflowChipseq.groovy b/lib/WorkflowChipseq.groovy index 25175f72..78e2752d 100755 --- a/lib/WorkflowChipseq.groovy +++ b/lib/WorkflowChipseq.groovy @@ -11,6 +11,7 @@ class WorkflowChipseq { // Check and validate parameters // public static void initialise(params, log) { + genomeExistsError(params, log) @@ -46,15 +47,57 @@ class WorkflowChipseq { return yaml_file_text } - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml) { + // + // Generate methods description for MultiQC + // + + public static String toolCitationText(params) { + + // TODO Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text + } + + public static String toolBibliographyText(params) { + + // TODO Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "

  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text + } + + public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file def meta = [:] meta.workflow = run_workflow.toMap() meta["manifest_map"] = run_workflow.manifest.toMap() + // Pipeline DOI meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + //meta["tool_bibliography"] = toolBibliographyText(params) + + def methods_text = mqc_methods_yaml.text def engine = new SimpleTemplateEngine() diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 6e8af09f..2894a678 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -20,40 +20,11 @@ class WorkflowMain { " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" } - // - // Generate help string - // - public static String help(workflow, params) { - def command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - def help_string = '' - help_string += NfcoreTemplate.logo(workflow, params.monochrome_logs) - help_string += NfcoreSchema.paramsHelp(workflow, params, command) - help_string += '\n' + citation(workflow) + '\n' - help_string += NfcoreTemplate.dashedLine(params.monochrome_logs) - return help_string - } - - // - // Generate parameter summary log string - // - public static String paramsSummaryLog(workflow, params) { - def summary_log = '' - summary_log += NfcoreTemplate.logo(workflow, params.monochrome_logs) - summary_log += NfcoreSchema.paramsSummaryLog(workflow, params) - summary_log += '\n' + citation(workflow) + '\n' - summary_log += NfcoreTemplate.dashedLine(params.monochrome_logs) - return summary_log - } // // Validate parameters and print summary to screen // public static void initialise(workflow, params, log) { - // Print help to screen if required - if (params.help) { - log.info help(workflow, params) - System.exit(0) - } // Print workflow version and exit on --version if (params.version) { @@ -62,14 +33,6 @@ class WorkflowMain { System.exit(0) } - // Print parameter summary log to screen - log.info paramsSummaryLog(workflow, params) - - // Validate workflow parameters via the JSON schema - if (params.validate_params) { - NfcoreSchema.validateParameters(workflow, params, log) - } - // Check that a -profile or Nextflow config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) diff --git a/main.nf b/main.nf index cf50b118..527da0e2 100644 --- a/main.nf +++ b/main.nf @@ -25,6 +25,22 @@ params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +include { validateParameters; paramsHelp } from 'plugin/nf-validation' + +// Print help message if needed +if (params.help) { + def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) + def citation = '\n' + WorkflowMain.citation(workflow) + '\n' + def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" + log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) + System.exit(0) +} + +// Validate input parameters +if (params.validate_params) { + validateParameters() +} + WorkflowMain.initialise(workflow, params, log) /* diff --git a/nextflow.config b/nextflow.config index 700f3a20..2ffd145a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -12,12 +12,12 @@ params { // TODO nf-core: Specify your pipeline's command line flags // Input options input = null - - // References genome = null igenomes_base = 's3://ngi-igenomes/igenomes' igenomes_ignore = false + + // MultiQC options multiqc_config = null multiqc_title = null @@ -27,7 +27,6 @@ params { // Boilerplate options outdir = null - tracedir = "${params.outdir}/pipeline_info" publish_dir_mode = 'copy' email = null email_on_fail = null @@ -36,19 +35,15 @@ params { hook_url = null help = false version = false - validate_params = true - show_hidden_params = false - schema_ignore_params = 'genomes' - // Config options + config_profile_name = null + config_profile_description = null custom_config_version = 'master' custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" - config_profile_description = null config_profile_contact = null config_profile_url = null - config_profile_name = null - + // Max resource options // Defaults only, expecting to be overwritten @@ -56,6 +51,13 @@ params { max_cpus = 16 max_time = '240.h' + // Schema validation default options + validationFailUnrecognisedParams = false + validationLenientMode = false + validationSchemaIgnoreParams = 'genomes' + validationShowHiddenParams = false + validate_params = true + } // Load base.config by default for all pipelines @@ -75,13 +77,11 @@ try { // } catch (Exception e) { // System.err.println("WARNING: Could not load nf-core/config/chipseq profiles: ${params.custom_config_base}/pipeline/chipseq.config") // } - - profiles { debug { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' - cleanup = false + cleanup = false } conda { conda.enabled = true @@ -104,7 +104,6 @@ profiles { } docker { docker.enabled = true - docker.registry = 'quay.io' docker.userEmulation = true conda.enabled = false singularity.enabled = false @@ -128,7 +127,6 @@ profiles { } podman { podman.enabled = true - podman.registry = 'quay.io' conda.enabled = false docker.enabled = false singularity.enabled = false @@ -172,6 +170,18 @@ profiles { test_full { includeConfig 'conf/test_full.config' } } +// Set default registry for Apptainer, Docker, Podman and Singularity independent of -profile +// Will not be used unless Apptainer / Docker / Podman / Singularity are enabled +// Set to your registry if you have a mirror of containers +apptainer.registry = 'quay.io' +docker.registry = 'quay.io' +podman.registry = 'quay.io' +singularity.registry = 'quay.io' + +// Nextflow plugins +plugins { + id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet +} // Load igenomes.config if required if (!params.igenomes_ignore) { @@ -179,8 +189,6 @@ if (!params.igenomes_ignore) { } else { params.genomes = [:] } - - // Export these variables to prevent local Python/R libraries from conflicting with those in the container // The JULIA depot path has been adjusted to a fixed path `/usr/local/share/julia` that needs to be used for packages in the container. // See https://apeltzer.github.io/post/03-julia-lang-nextflow/ for details on that. Once we have a common agreement on where to keep Julia packages, this is adjustable. @@ -198,19 +206,19 @@ process.shell = ['/bin/bash', '-euo', 'pipefail'] def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true - file = "${params.tracedir}/execution_timeline_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html" } report { enabled = true - file = "${params.tracedir}/execution_report_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html" } trace { enabled = true - file = "${params.tracedir}/execution_trace_${trace_timestamp}.txt" + file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt" } dag { enabled = true - file = "${params.tracedir}/pipeline_dag_${trace_timestamp}.html" + file = "${params.outdir}/pipeline_info/pipeline_dag_${trace_timestamp}.html" } manifest { @@ -219,7 +227,7 @@ manifest { homePage = 'https://github.com/nf-core/chipseq' description = """ChIP-seq peak-calling and differential analysis pipeline.""" mainScript = 'main.nf' - nextflowVersion = '!>=22.10.1' + nextflowVersion = '!>=23.04.0' version = '2.1.0dev' doi = '' } diff --git a/nextflow_schema.json b/nextflow_schema.json index 29c14519..0294f6f7 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -15,9 +15,9 @@ "input": { "type": "string", "format": "file-path", + "exists": true, "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", - "schema": "assets/schema_input.json", "description": "Path to comma-separated file containing information about the samples in the experiment.", "help_text": "You will need to create a design file with information about the samples in your experiment before running the pipeline. Use this parameter to specify its location. It has to be a comma-separated file with 3 columns, and a header row. See [usage docs](https://nf-co.re/chipseq/usage#samplesheet-input).", "fa_icon": "fas fa-file-csv" @@ -57,6 +57,7 @@ "fasta": { "type": "string", "format": "file-path", + "exists": true, "mimetype": "text/plain", "pattern": "^\\S+\\.fn?a(sta)?(\\.gz)?$", "description": "Path to FASTA genome file.", @@ -157,7 +158,7 @@ "description": "Maximum amount of time that can be requested for any single job.", "default": "240.h", "fa_icon": "far fa-clock", - "pattern": "^(\\d+\\.?\\s*(s|m|h|day)\\s*)+$", + "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", "hidden": true, "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" } @@ -174,12 +175,14 @@ "type": "boolean", "description": "Display help text.", "fa_icon": "fas fa-question-circle", + "default": false, "hidden": true }, "version": { "type": "boolean", "description": "Display version and exit.", "fa_icon": "fas fa-question-circle", + "default": false, "hidden": true }, "publish_dir_mode": { @@ -203,6 +206,7 @@ "type": "boolean", "description": "Send plain-text email instead of HTML.", "fa_icon": "fas fa-remove-format", + "default": false, "hidden": true }, "max_multiqc_email_size": { @@ -217,6 +221,7 @@ "type": "boolean", "description": "Do not use coloured log outputs.", "fa_icon": "fas fa-palette", + "default": false, "hidden": true }, "hook_url": { @@ -228,6 +233,7 @@ }, "multiqc_config": { "type": "string", + "format": "file-path", "description": "Custom config file to supply to MultiQC.", "fa_icon": "fas fa-cog", "hidden": true @@ -243,13 +249,6 @@ "description": "Custom MultiQC yaml file containing HTML including a methods description.", "fa_icon": "fas fa-cog" }, - "tracedir": { - "type": "string", - "description": "Directory to keep pipeline Nextflow logs and reports.", - "default": "${params.outdir}/pipeline_info", - "fa_icon": "fas fa-cogs", - "hidden": true - }, "validate_params": { "type": "boolean", "description": "Boolean whether to validate parameters against the schema at runtime", @@ -257,12 +256,29 @@ "fa_icon": "fas fa-check-square", "hidden": true }, - "show_hidden_params": { + "validationShowHiddenParams": { "type": "boolean", "fa_icon": "far fa-eye-slash", "description": "Show all params when using `--help`", + "default": false, "hidden": true, "help_text": "By default, parameters set as _hidden_ in the schema are not shown on the command line when a user runs with `--help`. Specifying this option will tell the pipeline to show all parameters." + }, + "validationFailUnrecognisedParams": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters fails when an unrecognised parameter is found.", + "default": false, + "hidden": true, + "help_text": "By default, when an unrecognised parameter is found, it returns a warinig." + }, + "validationLenientMode": { + "type": "boolean", + "fa_icon": "far fa-check-circle", + "description": "Validation of parameters in lenient more.", + "default": false, + "hidden": true, + "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." } } } diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index f43f0a6f..fe5b023e 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -1,21 +1,19 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE INPUTS + PRINT PARAMS SUMMARY ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -def summary_params = NfcoreSchema.paramsSummaryMap(workflow, params) +include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' -// Validate input parameters -WorkflowChipseq.initialise(params, log) +def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) +def citation = '\n' + WorkflowMain.citation(workflow) + '\n' +def summary_params = paramsSummaryMap(workflow) -// TODO nf-core: Add all file path parameters for the pipeline to the list below -// Check input path parameters to see if they exist -def checkPathParamList = [ params.input, params.multiqc_config, params.fasta ] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } +// Print parameter summary log to screen +log.info logo + paramsSummaryLog(workflow) + citation -// Check mandatory parameters -if (params.input) { ch_input = file(params.input) } else { exit 1, 'Input samplesheet not specified!' } +WorkflowChipseq.initialise(params, log) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -69,9 +67,12 @@ workflow CHIPSEQ { // SUBWORKFLOW: Read in samplesheet, validate and stage input files // INPUT_CHECK ( - ch_input + file(params.input) ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) + // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") + // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ + // ! There is currently no tooling to help you write a sample sheet schema // // MODULE: Run FastQC @@ -91,7 +92,7 @@ workflow CHIPSEQ { workflow_summary = WorkflowChipseq.paramsSummaryMultiqc(workflow, summary_params) ch_workflow_summary = Channel.value(workflow_summary) - methods_description = WorkflowChipseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description) + methods_description = WorkflowChipseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) ch_methods_description = Channel.value(methods_description) ch_multiqc_files = Channel.empty() From b8338993d403940ab669d6aa56587ad4240fbe74 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 3 Jul 2023 10:27:37 +0200 Subject: [PATCH 110/201] Fix multiqc_config --- assets/multiqc_config.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 8365cfde..1a6a1dbf 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > This report has been generated by the nf-core/chipseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. data_format: "yaml" From 7aeb56d179719a7760d19477ddd2a1a4f8264c5d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 3 Jul 2023 12:13:48 +0200 Subject: [PATCH 111/201] Reformat --- workflows/chipseq.nf | 7 ++++--- 1 file changed, 4 insertions(+), 3 deletions(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 4aacce3c..93f7911e 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -3,19 +3,20 @@ PRINT PARAMS SUMMARY ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ + include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) def citation = '\n' + WorkflowMain.citation(workflow) + '\n' def summary_params = paramsSummaryMap(workflow) +// Print parameter summary log to screen +log.info logo + paramsSummaryLog(workflow) + citation + def valid_params = [ aligners : [ 'bwa', 'bowtie2', 'chromap', 'star' ] ] -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - // Validate input parameters WorkflowChipseq.initialise(params, log, valid_params) From 92bc1be0da868d6bfa6c5ad8f950bbe1f8002ca7 Mon Sep 17 00:00:00 2001 From: bjlang <> Date: Mon, 17 Jul 2023 18:50:11 +0200 Subject: [PATCH 112/201] Allow empty lines in input samplesheet --- bin/check_samplesheet.py | 161 ++++++++++++++++++++------------------- 1 file changed, 82 insertions(+), 79 deletions(-) diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index b7b285ab..7005bb9e 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -59,96 +59,99 @@ def check_samplesheet(file_in, file_out): ## Check sample entries for line in fin: - lspl = [x.strip().strip('"') for x in line.strip().split(",")] - - # Check valid number of columns per row - if len(lspl) < len(HEADER): - print_error( - "Invalid number of columns (minimum = {})!".format(len(HEADER)), - "Line", - line, - ) - num_cols = len([x for x in lspl if x]) - if num_cols < MIN_COLS: - print_error( - "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), - "Line", - line, - ) - - ## Check sample name entries - sample, fastq_1, fastq_2, replicate, antibody, control, control_replicate = lspl[: len(HEADER)] - if sample.find(" ") != -1: - print(f"WARNING: Spaces have been replaced by underscores for sample: {sample}") - sample = sample.replace(" ", "_") - if not sample: - print_error("Sample entry has not been specified!", "Line", line) - - ## Check FastQ file extension - for fastq in [fastq_1, fastq_2]: - if fastq: - if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", "Line", line) - if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): - print_error( - "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", - "Line", - line, - ) + if line.strip(): + lspl = [x.strip().strip('"') for x in line.strip().split(",")] - ## Check replicate column is integer - if not replicate.isdecimal(): - print_error("Replicate id not an integer!", "Line", line) - sys.exit(1) - - ## Check antibody and control columns have valid values - if antibody: - if antibody.find(" ") != -1: - print(f"WARNING: Spaces have been replaced by underscores for antibody: {antibody}") - antibody = antibody.replace(" ", "_") - if not control: + # Check valid number of columns per row + if len(lspl) < len(HEADER): print_error( - "Both antibody and control columns must be specified!", + "Invalid number of columns (minimum = {})!".format(len(HEADER)), "Line", line, ) - - if control: - if control.find(" ") != -1: - print(f"WARNING: Spaces have been replaced by underscores for control: {control}") - control = control.replace(" ", "_") - if not control_replicate.isdecimal(): - print_error("Control replicate id not an integer!", "Line", line) - sys.exit(1) - control = "{}_REP{}".format(control, control_replicate) - if not antibody: + num_cols = len([x for x in lspl[: len(HEADER)] if x]) + if num_cols < MIN_COLS: print_error( - "Both antibody and control columns must be specified!", + "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), "Line", line, ) - ## Auto-detect paired-end/single-end - sample_info = [] ## [single_end, fastq_1, fastq_2, replicate, antibody, control] - if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ["0", fastq_1, fastq_2, replicate, antibody, control] - elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ["1", fastq_1, fastq_2, replicate, antibody, control] - else: - print_error("Invalid combination of columns provided!", "Line", line) - - ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, replicate, antibody, control ]]} - replicate = int(replicate) - sample_info = sample_info + lspl[len(HEADER) :] - if sample not in sample_mapping_dict: - sample_mapping_dict[sample] = {} - if replicate not in sample_mapping_dict[sample]: - sample_mapping_dict[sample][replicate] = [sample_info] - else: - if sample_info in sample_mapping_dict[sample][replicate]: - print_error("Samplesheet contains duplicate rows!", "Line", line) + ## Check sample name entries + sample, fastq_1, fastq_2, replicate, antibody, control, control_replicate = lspl[: len(HEADER)] + if sample.find(" ") != -1: + print(f"WARNING: Spaces have been replaced by underscores for sample: {sample}") + sample = sample.replace(" ", "_") + if not sample: + print_error("Sample entry has not been specified!", "Line", line) + + ## Check FastQ file extension + for fastq in [fastq_1, fastq_2]: + if fastq: + if fastq.find(" ") != -1: + print_error("FastQ file contains spaces!", "Line", line) + if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): + print_error( + "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", + "Line", + line, + ) + + ## Check replicate column is integer + if not replicate.isdecimal(): + print_error("Replicate id not an integer!", "Line", line) + sys.exit(1) + + ## Check antibody and control columns have valid values + if antibody: + if antibody.find(" ") != -1: + print(f"WARNING: Spaces have been replaced by underscores for antibody: {antibody}") + antibody = antibody.replace(" ", "_") + if not control: + print_error( + "Both antibody and control columns must be specified!", + "Line", + line, + ) + + if control: + if control.find(" ") != -1: + print(f"WARNING: Spaces have been replaced by underscores for control: {control}") + control = control.replace(" ", "_") + if not control_replicate.isdecimal(): + print_error("Control replicate id not an integer!", "Line", line) + sys.exit(1) + control = "{}_REP{}".format(control, control_replicate) + if not antibody: + print_error( + "Both antibody and control columns must be specified!", + "Line", + line, + ) + + ## Auto-detect paired-end/single-end + sample_info = [] ## [single_end, fastq_1, fastq_2, replicate, antibody, control] + ## Paired-end short reads + if sample and fastq_1 and fastq_2: + sample_info = ["0", fastq_1, fastq_2, replicate, antibody, control] + ## Single-end short reads + elif sample and fastq_1 and not fastq_2: + sample_info = ["1", fastq_1, fastq_2, replicate, antibody, control] + else: + print_error("Invalid combination of columns provided!", "Line", line) + + ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, replicate, antibody, control ]]} + replicate = int(replicate) + sample_info = sample_info + lspl[len(HEADER) :] + if sample not in sample_mapping_dict: + sample_mapping_dict[sample] = {} + if replicate not in sample_mapping_dict[sample]: + sample_mapping_dict[sample][replicate] = [sample_info] else: - sample_mapping_dict[sample][replicate].append(sample_info) + if sample_info in sample_mapping_dict[sample][replicate]: + print_error("Samplesheet contains duplicate rows!", "Line", line) + else: + sample_mapping_dict[sample][replicate].append(sample_info) ## Write validated samplesheet with appropriate columns if len(sample_mapping_dict) > 0: From fa3221eb05e03e1e9a230642f239a35abfa4983a Mon Sep 17 00:00:00 2001 From: bjlang <> Date: Wed, 19 Jul 2023 12:01:46 +0200 Subject: [PATCH 113/201] Remove quay.io from biocontainer address --- modules/local/bam_remove_orphans.nf | 2 +- modules/local/bamtools_filter.nf | 2 +- modules/local/bedtools_genomecov.nf | 2 +- modules/local/deseq2_qc.nf | 2 +- modules/local/frip_score.nf | 2 +- modules/local/genome_blacklist_regions.nf | 2 +- modules/local/gtf2bed.nf | 2 +- modules/local/igv.nf | 2 +- modules/local/macs2_consensus.nf | 2 +- modules/local/multiqc.nf | 2 +- modules/local/multiqc_custom_peaks.nf | 4 +++- modules/local/multiqc_custom_phantompeakqualtools.nf | 2 +- modules/local/plot_homer_annotatepeaks.nf | 2 +- modules/local/plot_macs2_qc.nf | 2 +- modules/local/star_align.nf | 2 +- modules/local/star_genomegenerate.nf | 2 +- 16 files changed, 18 insertions(+), 16 deletions(-) diff --git a/modules/local/bam_remove_orphans.nf b/modules/local/bam_remove_orphans.nf index d6e221aa..2bfb7dfd 100644 --- a/modules/local/bam_remove_orphans.nf +++ b/modules/local/bam_remove_orphans.nf @@ -8,7 +8,7 @@ process BAM_REMOVE_ORPHANS { conda "bioconda::pysam=0.19.0 bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-57736af1eb98c01010848572c9fec9fff6ffaafd:402e865b8f6af2f3e58c6fc8d57127ff0144b2c7-0' : - 'quay.io/biocontainers/mulled-v2-57736af1eb98c01010848572c9fec9fff6ffaafd:402e865b8f6af2f3e58c6fc8d57127ff0144b2c7-0' }" + 'biocontainers/mulled-v2-57736af1eb98c01010848572c9fec9fff6ffaafd:402e865b8f6af2f3e58c6fc8d57127ff0144b2c7-0' }" input: tuple val(meta), path(bam) diff --git a/modules/local/bamtools_filter.nf b/modules/local/bamtools_filter.nf index c1e750d7..a1e8feed 100644 --- a/modules/local/bamtools_filter.nf +++ b/modules/local/bamtools_filter.nf @@ -5,7 +5,7 @@ process BAMTOOLS_FILTER { conda "bioconda::bamtools=2.5.2 bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' : - 'quay.io/biocontainers/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' }" + 'biocontainers/mulled-v2-0560a8046fc82aa4338588eca29ff18edab2c5aa:5687a7da26983502d0a8a9a6b05ed727c740ddc4-0' }" input: tuple val(meta), path(bam), path(bai) diff --git a/modules/local/bedtools_genomecov.nf b/modules/local/bedtools_genomecov.nf index 28dcc5dd..fb274631 100644 --- a/modules/local/bedtools_genomecov.nf +++ b/modules/local/bedtools_genomecov.nf @@ -5,7 +5,7 @@ process BEDTOOLS_GENOMECOV { conda "bioconda::bedtools=2.30.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0': - 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" + 'biocontainers/bedtools:2.30.0--hc088bd4_0' }" input: tuple val(meta), path(bam), path(flagstat) diff --git a/modules/local/deseq2_qc.nf b/modules/local/deseq2_qc.nf index d9b96e11..14b57f69 100644 --- a/modules/local/deseq2_qc.nf +++ b/modules/local/deseq2_qc.nf @@ -7,7 +7,7 @@ process DESEQ2_QC { conda "conda-forge::r-base bioconda::bioconductor-deseq2 bioconda::bioconductor-biocparallel bioconda::bioconductor-tximport bioconda::bioconductor-complexheatmap conda-forge::r-optparse conda-forge::r-ggplot2 conda-forge::r-rcolorbrewer conda-forge::r-pheatmap" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' : - 'quay.io/biocontainers/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' }" + 'biocontainers/mulled-v2-8849acf39a43cdd6c839a369a74c0adc823e2f91:ab110436faf952a33575c64dd74615a84011450b-0' }" input: tuple val(meta), path(counts) diff --git a/modules/local/frip_score.nf b/modules/local/frip_score.nf index 28133e0c..77f856bc 100644 --- a/modules/local/frip_score.nf +++ b/modules/local/frip_score.nf @@ -5,7 +5,7 @@ process FRIP_SCORE { conda "bioconda::bedtools=2.30.0 bioconda::samtools=1.15.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-8186960447c5cb2faa697666dc1e6d919ad23f3e:3127fcae6b6bdaf8181e21a26ae61231030a9fcb-0': - 'quay.io/biocontainers/mulled-v2-8186960447c5cb2faa697666dc1e6d919ad23f3e:3127fcae6b6bdaf8181e21a26ae61231030a9fcb-0' }" + 'biocontainers/mulled-v2-8186960447c5cb2faa697666dc1e6d919ad23f3e:3127fcae6b6bdaf8181e21a26ae61231030a9fcb-0' }" input: tuple val(meta), path(bam), path(peak) diff --git a/modules/local/genome_blacklist_regions.nf b/modules/local/genome_blacklist_regions.nf index fcb8451c..c365c8a7 100644 --- a/modules/local/genome_blacklist_regions.nf +++ b/modules/local/genome_blacklist_regions.nf @@ -7,7 +7,7 @@ process GENOME_BLACKLIST_REGIONS { conda "bioconda::bedtools=2.30.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/bedtools:2.30.0--hc088bd4_0': - 'quay.io/biocontainers/bedtools:2.30.0--hc088bd4_0' }" + 'biocontainers/bedtools:2.30.0--hc088bd4_0' }" input: path sizes diff --git a/modules/local/gtf2bed.nf b/modules/local/gtf2bed.nf index 8a8e7bf9..bc0eef54 100644 --- a/modules/local/gtf2bed.nf +++ b/modules/local/gtf2bed.nf @@ -5,7 +5,7 @@ process GTF2BED { conda "conda-forge::perl=5.26.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/perl:5.26.2': - 'quay.io/biocontainers/perl:5.26.2' }" + 'biocontainers/perl:5.26.2' }" input: path gtf diff --git a/modules/local/igv.nf b/modules/local/igv.nf index cf02fd9e..863af845 100644 --- a/modules/local/igv.nf +++ b/modules/local/igv.nf @@ -6,7 +6,7 @@ process IGV { conda "conda-forge::python=3.8.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/python:3.8.3': - 'quay.io/biocontainers/python:3.8.3' }" + 'biocontainers/python:3.8.3' }" input: val aligner_dir diff --git a/modules/local/macs2_consensus.nf b/modules/local/macs2_consensus.nf index eb373d2c..6b2006e4 100644 --- a/modules/local/macs2_consensus.nf +++ b/modules/local/macs2_consensus.nf @@ -8,7 +8,7 @@ process MACS2_CONSENSUS { conda "conda-forge::biopython conda-forge::r-optparse=1.7.1 conda-forge::r-upsetr=1.4.0 bioconda::bedtools=2.30.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-2f48cc59b03027e31ead6d383fe1b8057785dd24:5d182f583f4696f4c4d9f3be93052811b383341f-0': - 'quay.io/biocontainers/mulled-v2-2f48cc59b03027e31ead6d383fe1b8057785dd24:5d182f583f4696f4c4d9f3be93052811b383341f-0' }" + 'biocontainers/mulled-v2-2f48cc59b03027e31ead6d383fe1b8057785dd24:5d182f583f4696f4c4d9f3be93052811b383341f-0' }" input: tuple val(meta), path(peaks) diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index d23d2cbe..32c35df7 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -4,7 +4,7 @@ process MULTIQC { conda "bioconda::multiqc=1.13a" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/multiqc:1.13a--pyhdfd78af_1': - 'quay.io/biocontainers/multiqc:1.13a--pyhdfd78af_1' }" + 'biocontainers/multiqc:1.13a--pyhdfd78af_1' }" input: path multiqc_config diff --git a/modules/local/multiqc_custom_peaks.nf b/modules/local/multiqc_custom_peaks.nf index c19d81a5..eb3e5cbb 100644 --- a/modules/local/multiqc_custom_peaks.nf +++ b/modules/local/multiqc_custom_peaks.nf @@ -1,9 +1,10 @@ process MULTIQC_CUSTOM_PEAKS { tag "$meta.id" + conda "conda-forge::sed=4.7" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/ubuntu:20.04' : - 'docker.io/library/ubuntu:20.04' }" + 'nf-core/ubuntu:20.04' }" input: tuple val(meta), path(peak), path(frip) @@ -13,6 +14,7 @@ process MULTIQC_CUSTOM_PEAKS { output: tuple val(meta), path("*.peak_count_mqc.tsv"), emit: count tuple val(meta), path("*.FRiP_mqc.tsv") , emit: frip + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/local/multiqc_custom_phantompeakqualtools.nf b/modules/local/multiqc_custom_phantompeakqualtools.nf index 9e59d057..c9239bde 100644 --- a/modules/local/multiqc_custom_phantompeakqualtools.nf +++ b/modules/local/multiqc_custom_phantompeakqualtools.nf @@ -3,7 +3,7 @@ process MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS { conda "conda-forge::r-base=3.5.1" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/r-base:3.5.1': - 'quay.io/biocontainers/r-base:3.5.1' }" + 'biocontainers/r-base:3.5.1' }" input: tuple val(meta), path(spp), path(rdata) diff --git a/modules/local/plot_homer_annotatepeaks.nf b/modules/local/plot_homer_annotatepeaks.nf index 36c4730e..ef4b1ce6 100644 --- a/modules/local/plot_homer_annotatepeaks.nf +++ b/modules/local/plot_homer_annotatepeaks.nf @@ -4,7 +4,7 @@ process PLOT_HOMER_ANNOTATEPEAKS { conda "conda-forge::r-base=4.0.3 conda-forge::r-reshape2=1.4.4 conda-forge::r-optparse=1.6.6 conda-forge::r-ggplot2=3.3.3 conda-forge::r-scales=1.1.1 conda-forge::r-viridis=0.5.1 conda-forge::r-tidyverse=1.3.0 bioconda::bioconductor-biostrings=2.58.0 bioconda::bioconductor-complexheatmap=2.6.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0': - 'quay.io/biocontainers/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0' }" + 'biocontainers/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0' }" input: path annos diff --git a/modules/local/plot_macs2_qc.nf b/modules/local/plot_macs2_qc.nf index 7599c9f8..d514db46 100644 --- a/modules/local/plot_macs2_qc.nf +++ b/modules/local/plot_macs2_qc.nf @@ -4,7 +4,7 @@ process PLOT_MACS2_QC { conda "conda-forge::r-base=4.0.3 conda-forge::r-reshape2=1.4.4 conda-forge::r-optparse=1.6.6 conda-forge::r-ggplot2=3.3.3 conda-forge::r-scales=1.1.1 conda-forge::r-viridis=0.5.1 conda-forge::r-tidyverse=1.3.0 bioconda::bioconductor-biostrings=2.58.0 bioconda::bioconductor-complexheatmap=2.6.2" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0': - 'quay.io/biocontainers/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0' }" + 'biocontainers/mulled-v2-ad9dd5f398966bf899ae05f8e7c54d0fb10cdfa7:05678da05b8e5a7a5130e90a9f9a6c585b965afa-0' }" input: path peaks diff --git a/modules/local/star_align.nf b/modules/local/star_align.nf index ec7fc2af..6a45b731 100644 --- a/modules/local/star_align.nf +++ b/modules/local/star_align.nf @@ -6,7 +6,7 @@ process STAR_ALIGN { conda "bioconda::star=2.6.1d" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/star:2.6.1d--0' : - 'quay.io/biocontainers/star:2.6.1d--0' }" + 'biocontainers/star:2.6.1d--0' }" input: tuple val(meta) , path(reads) diff --git a/modules/local/star_genomegenerate.nf b/modules/local/star_genomegenerate.nf index 285df97c..a36af0a8 100644 --- a/modules/local/star_genomegenerate.nf +++ b/modules/local/star_genomegenerate.nf @@ -6,7 +6,7 @@ process STAR_GENOMEGENERATE { conda "bioconda::star=2.6.1d bioconda::samtools=1.10 conda-forge::gawk=5.1.0" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0' : - 'quay.io/biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0' }" + 'biocontainers/mulled-v2-1fa26d1ce03c295fe2fdcf85831a92fbcbd7e8c2:59cdd445419f14abac76b31dd0d71217994cbcc9-0' }" input: path fasta From 48c10a7802bb6368571dd0a704f36cb8f85eead3 Mon Sep 17 00:00:00 2001 From: Adam Talbot <12817534+adamrtalbot@users.noreply.github.com> Date: Fri, 21 Jul 2023 17:01:50 +0100 Subject: [PATCH 114/201] Add missing comma to awsfulltest --- .github/workflows/awsfulltest.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 4833b93a..4a029854 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -27,7 +27,7 @@ jobs: parameters: | { "hook_url": "${{ secrets.MEGATESTS_ALERTS_SLACK_HOOK_URL }}", - "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/chipseq/results-${{ github.sha }}" + "outdir": "s3://${{ secrets.AWS_S3_BUCKET }}/chipseq/results-${{ github.sha }}", "aligner": "${{ matrix.aligner }}" } profiles: test_full From e5db23920da2e196a2a54a8df9c9f681863d9c55 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Samuel=20Ruiz=20P=C3=A9rez?= Date: Sun, 10 Sep 2023 21:57:42 +0200 Subject: [PATCH 115/201] Fix publishDir paths in modules.config --- assets/multiqc_config.yml | 8 ++++---- conf/modules.config | 34 +++++++++++++++++----------------- modules/local/igv.nf | 8 ++++---- modules/local/multiqc.nf | 16 ++++++++-------- workflows/chipseq.nf | 2 +- 5 files changed, 34 insertions(+), 34 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 1a6a1dbf..6f192e4f 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -44,12 +44,12 @@ module_order: name: "MERGED LIB: SAMTools (unfiltered)" info: "This section of the report shows SAMTools results after merging libraries and before filtering." path_filters: - - "./alignment/mergedLibrary/unfiltered/*.mLb.mkD.sorted.bam*" + - "./alignment/merged_library/unfiltered/*.mLb.mkD.sorted.bam*" - picard: name: "MERGED LIB: Picard (unfiltered)" info: "This section of the report shows picard results after merging libraries and before filtering." path_filters: - - "./alignment/mergedLibrary/unfiltered/picard_metrics/*" + - "./alignment/merged_library/unfiltered/picard_metrics/*" - preseq: name: "MERGED LIB: Preseq (unfiltered)" info: "This section of the report shows Preseq results after merging libraries and before filtering." @@ -57,12 +57,12 @@ module_order: name: "MERGED LIB: SAMTools (filtered)" info: "This section of the report shows SAMTools results after merging libraries and after filtering." path_filters: - - "./alignment/mergedLibrary/filtered/*.mLb.clN.sorted.bam*" + - "./alignment/merged_library/filtered/*.mLb.clN.sorted.bam*" - picard: name: "MERGED LIB: Picard (filtered)" info: "This section of the report shows picard results after merging libraries and after filtering." path_filters: - - "./alignment/mergedLibrary/filtered/picard_metrics/*" + - "./alignment/merged_library/filtered/picard_metrics/*" - deeptools: name: "MERGED LIB: deepTools" anchor: "mlib_deeptools" diff --git a/conf/modules.config b/conf/modules.config index 6b67b9aa..995f95e1 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -329,12 +329,12 @@ process { ext.prefix = { "${meta.id}.mLb.mkD.sorted" } publishDir = [ [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/picard_metrics" }, + path: { "${params.outdir}/${params.aligner}/merged_library/picard_metrics" }, mode: params.publish_dir_mode, pattern: '*.metrics.txt' ], [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, + path: { "${params.outdir}/${params.aligner}/merged_library" }, mode: params.publish_dir_mode, pattern: '*.bam', enabled: params.save_align_intermeds @@ -344,7 +344,7 @@ process { withName: '.*:BAM_MARKDUPLICATES_PICARD:SAMTOOLS_INDEX' { publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, + path: { "${params.outdir}/${params.aligner}/merged_library" }, mode: params.publish_dir_mode, pattern: '*.{bai,csi}', enabled: params.save_align_intermeds @@ -354,7 +354,7 @@ process { withName: '.*:BAM_MARKDUPLICATES_PICARD:BAM_STATS_SAMTOOLS:.*' { ext.prefix = { "${meta.id}.mLb.mkD.sorted.bam" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary/samtools_stats" }, + path: { "${params.outdir}/${params.aligner}/merged_library/samtools_stats" }, mode: params.publish_dir_mode, pattern: '*.{stats,flagstat,idxstats}' ] @@ -392,7 +392,7 @@ process { ext.args = '-n' ext.prefix = { "${meta.id}.mLb.flT.name_sorted" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/mergedLibrary" }, + path: { "${params.outdir}/${params.aligner}/merged_library" }, mode: params.publish_dir_mode, pattern: '*.bam', enabled: params.save_align_intermeds @@ -559,7 +559,7 @@ process { publishDir = [ path: { [ "${params.outdir}/${params.aligner}/merged_library/macs2", - params.narrow_peak? '/narrowPeak' : '/broadPeak' + params.narrow_peak? '/narrow_peak' : '/broad_peak' ].join('') }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } @@ -569,14 +569,14 @@ process { withName: 'FRIP_SCORE' { ext.args = '-bed -c -f 0.20' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, enabled: false ] } withName: 'MULTIQC_CUSTOM_PEAKS' { publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak} ? '/narrowPeak' : '/broadPeak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -589,7 +589,7 @@ if (!params.skip_peak_annotation) { ext.args = '-gid' ext.prefix = { "${meta.id}_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak' }" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -611,7 +611,7 @@ if (!params.skip_peak_annotation) { ext.args = '-o ./' ext.prefix = 'macs2_annotatePeaks' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -626,7 +626,7 @@ if (!params.skip_consensus_peaks) { ext.when = { meta.multiple_groups || meta.replicates_exist } ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -636,7 +636,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-F SAF -O --fracOverlap 0.2' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -649,7 +649,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-gid' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -658,7 +658,7 @@ if (!params.skip_consensus_peaks) { withName: 'ANNOTATE_BOOLEAN_PEAKS' { ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -678,7 +678,7 @@ if (!params.skip_consensus_peaks) { ].join(' ').trim() ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}/consensus/${meta.id}/deseq2" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}/deseq2" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -692,7 +692,7 @@ if (!params.skip_igv) { withName: 'IGV' { publishDir = [ [ - path: { "${params.outdir}/igv/${params.narrow_peak? '/narrowPeak' : '/broadPeak' }" }, + path: { "${params.outdir}/igv/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, mode: params.publish_dir_mode, pattern: '*.{txt,xml}' ], @@ -711,7 +711,7 @@ if (!params.skip_multiqc) { withName: 'MULTIQC' { ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' publishDir = [ - path: { "${params.outdir}/multiqc/${params.narrow_peak ? '/narrowPeak' : '/broadPeak'}" }, + path: { "${params.outdir}/multiqc/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/modules/local/igv.nf b/modules/local/igv.nf index cf02fd9e..0d08b203 100644 --- a/modules/local/igv.nf +++ b/modules/local/igv.nf @@ -12,9 +12,9 @@ process IGV { val aligner_dir val peak_dir path fasta - path ("${aligner_dir}/mergedLibrary/bigwig/*") - path ("${aligner_dir}/mergedLibrary/macs2/${peak_dir}/*") - path ("${aligner_dir}/mergedLibrary/macs2/${peak_dir}/consensus/*") + path ("${aligner_dir}/merged_library/bigwig/*") + path ("${aligner_dir}/merged_library/macs2/${peak_dir}/*") + path ("${aligner_dir}/merged_library/macs2/${peak_dir}/consensus/*") path ("mappings/*") output: @@ -27,7 +27,7 @@ process IGV { task.ext.when == null || task.ext.when script: // scripts are bundled with the pipeline in nf-core/chipseq/bin/ - def consensus_dir = "${aligner_dir}/mergedLibrary/macs2/${peak_dir}/consensus/*" + def consensus_dir = "${aligner_dir}/merged_library/macs2/${peak_dir}/consensus/*" """ find * -type l -name "*.bigWig" -exec echo -e ""{}"\\t0,0,178" \\; > bigwig.igv.txt find * -type l -name "*Peak" -exec echo -e ""{}"\\t0,0,178" \\; > peaks.igv.txt diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index d23d2cbe..9dab5448 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -22,15 +22,15 @@ process MULTIQC { path ('alignment/library/*') path ('alignment/library/*') - path ('alignment/mergedLibrary/unfiltered/*') - path ('alignment/mergedLibrary/unfiltered/*') - path ('alignment/mergedLibrary/unfiltered/*') - path ('alignment/mergedLibrary/unfiltered/picard_metrics/*') + path ('alignment/merged_library/unfiltered/*') + path ('alignment/merged_library/unfiltered/*') + path ('alignment/merged_library/unfiltered/*') + path ('alignment/merged_library/unfiltered/picard_metrics/*') - path ('alignment/mergedLibrary/filtered/*') - path ('alignment/mergedLibrary/filtered/*') - path ('alignment/mergedLibrary/filtered/*') - path ('alignment/mergedLibrary/filtered/picard_metrics/*') + path ('alignment/merged_library/filtered/*') + path ('alignment/merged_library/filtered/*') + path ('alignment/merged_library/filtered/*') + path ('alignment/merged_library/filtered/picard_metrics/*') path ('preseq/*') diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 93f7911e..054ac3da 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -692,7 +692,7 @@ workflow CHIPSEQ { if (!params.skip_igv) { IGV ( params.aligner, - params.narrow_peak ? 'narrowPeak' : 'broadPeak', + params.narrow_peak ? 'narrow_peak' : 'broad_peak', PREPARE_GENOME.out.fasta, UCSC_BEDGRAPHTOBIGWIG.out.bigwig.collect{it[1]}.ifEmpty([]), ch_macs2_peaks.collect{it[1]}.ifEmpty([]), From 1ae46c80e157fd04f78d1746f4eb8b93eded15ca Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 25 Sep 2023 15:16:18 +0000 Subject: [PATCH 116/201] Template update for nf-core/tools version 2.10 --- .devcontainer/devcontainer.json | 1 + .github/CONTRIBUTING.md | 4 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcments.yml | 68 +++++++++ CITATIONS.md | 2 +- CODE_OF_CONDUCT.md | 133 ++++++++++++++---- README.md | 21 +-- assets/multiqc_config.yml | 4 +- conf/modules.config | 9 ++ docs/output.md | 5 +- docs/usage.md | 16 ++- lib/NfcoreTemplate.groovy | 16 +++ lib/WorkflowChipseq.groovy | 2 +- main.nf | 3 + modules.json | 6 +- .../custom/dumpsoftwareversions/main.nf | 2 +- modules/nf-core/fastqc/main.nf | 8 +- modules/nf-core/multiqc/main.nf | 2 +- nextflow.config | 7 +- nextflow_schema.json | 15 -- workflows/chipseq.nf | 1 + 21 files changed, 251 insertions(+), 76 deletions(-) create mode 100644 .github/workflows/release-announcments.yml diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index ea27a584..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -2,6 +2,7 @@ "name": "nfcore", "image": "nfcore/gitpod:latest", "remoteUser": "gitpod", + "runArgs": ["--privileged"], // Configure tool-specific properties. "customizations": { diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 8c24b7f1..75fde7dd 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,7 +9,9 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -> If you need help using or modifying nf-core/chipseq then the best place to ask is on the nf-core Slack [#chipseq](https://nfcore.slack.com/channels/chipseq) channel ([join our Slack here](https://nf-co.re/join/slack)). +:::info +If you need help using or modifying nf-core/chipseq then the best place to ask is on the nf-core Slack [#chipseq](https://nfcore.slack.com/channels/chipseq) channel ([join our Slack here](https://nf-co.re/join/slack)). +::: ## Contribution workflow diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 888cb4bc..b8bdd214 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -78,7 +78,7 @@ jobs: - uses: actions/setup-python@v4 with: - python-version: "3.8" + python-version: "3.11" architecture: "x64" - name: Install dependencies diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcments.yml new file mode 100644 index 00000000..6ad33927 --- /dev/null +++ b/.github/workflows/release-announcments.yml @@ -0,0 +1,68 @@ +name: release-announcements +# Automatic release toot and tweet anouncements +on: + release: + types: [published] + workflow_dispatch: + +jobs: + toot: + runs-on: ubuntu-latest + steps: + - uses: rzr/fediverse-action@master + with: + access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} + host: "mstdn.science" # custom host if not "mastodon.social" (default) + # GitHub event payload + # https://docs.github.com/en/developers/webhooks-and-events/webhooks/webhook-events-and-payloads#release + message: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + + send-tweet: + runs-on: ubuntu-latest + + steps: + - uses: actions/setup-python@v4 + with: + python-version: "3.10" + - name: Install dependencies + run: pip install tweepy==4.14.0 + - name: Send tweet + shell: python + run: | + import os + import tweepy + + client = tweepy.Client( + access_token=os.getenv("TWITTER_ACCESS_TOKEN"), + access_token_secret=os.getenv("TWITTER_ACCESS_TOKEN_SECRET"), + consumer_key=os.getenv("TWITTER_CONSUMER_KEY"), + consumer_secret=os.getenv("TWITTER_CONSUMER_SECRET"), + ) + tweet = os.getenv("TWEET") + client.create_tweet(text=tweet) + env: + TWEET: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + TWITTER_CONSUMER_KEY: ${{ secrets.TWITTER_CONSUMER_KEY }} + TWITTER_CONSUMER_SECRET: ${{ secrets.TWITTER_CONSUMER_SECRET }} + TWITTER_ACCESS_TOKEN: ${{ secrets.TWITTER_ACCESS_TOKEN }} + TWITTER_ACCESS_TOKEN_SECRET: ${{ secrets.TWITTER_ACCESS_TOKEN_SECRET }} + + bsky-post: + runs-on: ubuntu-latest + steps: + - uses: zentered/bluesky-post-action@v0.0.2 + with: + post: | + Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! + + Please see the changelog: ${{ github.event.release.html_url }} + env: + BSKY_IDENTIFIER: ${{ secrets.BSKY_IDENTIFIER }} + BSKY_PASSWORD: ${{ secrets.BSKY_PASSWORD }} + # diff --git a/CITATIONS.md b/CITATIONS.md index d47e0566..8c3593ec 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -12,7 +12,7 @@ - [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/) - > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. Available online https://www.bioinformatics.babraham.ac.uk/projects/fastqc/. + > Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online]. - [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/) diff --git a/CODE_OF_CONDUCT.md b/CODE_OF_CONDUCT.md index f4fd052f..c089ec78 100644 --- a/CODE_OF_CONDUCT.md +++ b/CODE_OF_CONDUCT.md @@ -1,18 +1,20 @@ -# Code of Conduct at nf-core (v1.0) +# Code of Conduct at nf-core (v1.4) ## Our Pledge -In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core, pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: +In the interest of fostering an open, collaborative, and welcoming environment, we as contributors and maintainers of nf-core pledge to making participation in our projects and community a harassment-free experience for everyone, regardless of: - Age +- Ability - Body size +- Caste - Familial status - Gender identity and expression - Geographical location - Level of experience - Nationality and national origins - Native language -- Physical and neurological ability +- Neurodiversity - Race or ethnicity - Religion - Sexual identity and orientation @@ -22,80 +24,133 @@ Please note that the list above is alphabetised and is therefore not ranked in a ## Preamble -> Note: This Code of Conduct (CoC) has been drafted by the nf-core Safety Officer and been edited after input from members of the nf-core team and others. "We", in this document, refers to the Safety Officer and members of the nf-core core team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will amended periodically to keep it up-to-date, and in case of any dispute, the most current version will apply. +:::note +This Code of Conduct (CoC) has been drafted by Renuka Kudva, Cris Tuñí, and Michael Heuer, with input from the nf-core Core Team and Susanna Marquez from the nf-core community. "We", in this document, refers to the Safety Officers and members of the nf-core Core Team, both of whom are deemed to be members of the nf-core community and are therefore required to abide by this Code of Conduct. This document will be amended periodically to keep it up-to-date. In case of any dispute, the most current version will apply. +::: -An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). Our current safety officer is Renuka Kudva. +An up-to-date list of members of the nf-core core team can be found [here](https://nf-co.re/about). + +Our Safety Officers are Saba Nafees, Cris Tuñí, and Michael Heuer. nf-core is a young and growing community that welcomes contributions from anyone with a shared vision for [Open Science Policies](https://www.fosteropenscience.eu/taxonomy/term/8). Open science policies encompass inclusive behaviours and we strive to build and maintain a safe and inclusive environment for all individuals. -We have therefore adopted this code of conduct (CoC), which we require all members of our community and attendees in nf-core events to adhere to in all our workspaces at all times. Workspaces include but are not limited to Slack, meetings on Zoom, Jitsi, YouTube live etc. +We have therefore adopted this CoC, which we require all members of our community and attendees of nf-core events to adhere to in all our workspaces at all times. Workspaces include, but are not limited to, Slack, meetings on Zoom, gather.town, YouTube live etc. -Our CoC will be strictly enforced and the nf-core team reserve the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. +Our CoC will be strictly enforced and the nf-core team reserves the right to exclude participants who do not comply with our guidelines from our workspaces and future nf-core activities. -We ask all members of our community to help maintain a supportive and productive workspace and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. +We ask all members of our community to help maintain supportive and productive workspaces and to avoid behaviours that can make individuals feel unsafe or unwelcome. Please help us maintain and uphold this CoC. -Questions, concerns or ideas on what we can include? Contact safety [at] nf-co [dot] re +Questions, concerns, or ideas on what we can include? Contact members of the Safety Team on Slack or email safety [at] nf-co [dot] re. ## Our Responsibilities -The safety officer is responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. +Members of the Safety Team (the Safety Officers) are responsible for clarifying the standards of acceptable behavior and are expected to take appropriate and fair corrective action in response to any instances of unacceptable behaviour. -The safety officer in consultation with the nf-core core team have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this Code of Conduct, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. +The Safety Team, in consultation with the nf-core core team, have the right and responsibility to remove, edit, or reject comments, commits, code, wiki edits, issues, and other contributions that are not aligned to this CoC, or to ban temporarily or permanently any contributor for other behaviors that they deem inappropriate, threatening, offensive, or harmful. -Members of the core team or the safety officer who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and be subject to the same actions as others in violation of the CoC. +Members of the core team or the Safety Team who violate the CoC will be required to recuse themselves pending investigation. They will not have access to any reports of the violations and will be subject to the same actions as others in violation of the CoC. -## When are where does this Code of Conduct apply? +## When and where does this Code of Conduct apply? -Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events. This includes but is not limited to the following listed alphabetically and therefore in no order of preference: +Participation in the nf-core community is contingent on following these guidelines in all our workspaces and events, such as hackathons, workshops, bytesize, and collaborative workspaces on gather.town. These guidelines include, but are not limited to, the following (listed alphabetically and therefore in no order of preference): - Communicating with an official project email address. - Communicating with community members within the nf-core Slack channel. - Participating in hackathons organised by nf-core (both online and in-person events). -- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence. -- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, Jitsi, YouTube live etc. +- Participating in collaborative work on GitHub, Google Suite, community calls, mentorship meetings, email correspondence, and on the nf-core gather.town workspace. +- Participating in workshops, training, and seminar series organised by nf-core (both online and in-person events). This applies to events hosted on web-based platforms such as Zoom, gather.town, Jitsi, YouTube live etc. - Representing nf-core on social media. This includes both official and personal accounts. ## nf-core cares 😊 -nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include but are not limited to the following (listed in alphabetical order): +nf-core's CoC and expectations of respectful behaviours for all participants (including organisers and the nf-core team) include, but are not limited to, the following (listed in alphabetical order): - Ask for consent before sharing another community member’s personal information (including photographs) on social media. - Be respectful of differing viewpoints and experiences. We are all here to learn from one another and a difference in opinion can present a good learning opportunity. -- Celebrate your accomplishments at events! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) +- Celebrate your accomplishments! (Get creative with your use of emojis 🎉 🥳 💯 🙌 !) - Demonstrate empathy towards other community members. (We don’t all have the same amount of time to dedicate to nf-core. If tasks are pending, don’t hesitate to gently remind members of your team. If you are leading a task, ask for help if you feel overwhelmed.) - Engage with and enquire after others. (This is especially important given the geographically remote nature of the nf-core community, so let’s do this the best we can) - Focus on what is best for the team and the community. (When in doubt, ask) -- Graciously accept constructive criticism, yet be unafraid to question, deliberate, and learn. +- Accept feedback, yet be unafraid to question, deliberate, and learn. - Introduce yourself to members of the community. (We’ve all been outsiders and we know that talking to strangers can be hard for some, but remember we’re interested in getting to know you and your visions for open science!) -- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communications to be kind.**) +- Show appreciation and **provide clear feedback**. (This is especially important because we don’t see each other in person and it can be harder to interpret subtleties. Also remember that not everyone understands a certain language to the same extent as you do, so **be clear in your communication to be kind.**) - Take breaks when you feel like you need them. -- Using welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack.) +- Use welcoming and inclusive language. (Participants are encouraged to display their chosen pronouns on Zoom or in communication on Slack) ## nf-core frowns on 😕 -The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this code of conduct. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces. +The following behaviours from any participants within the nf-core community (including the organisers) will be considered unacceptable under this CoC. Engaging or advocating for any of the following could result in expulsion from nf-core workspaces: - Deliberate intimidation, stalking or following and sustained disruption of communication among participants of the community. This includes hijacking shared screens through actions such as using the annotate tool in conferencing software such as Zoom. - “Doxing” i.e. posting (or threatening to post) another person’s personal identifying information online. - Spamming or trolling of individuals on social media. -- Use of sexual or discriminatory imagery, comments, or jokes and unwelcome sexual attention. -- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion or work experience. +- Use of sexual or discriminatory imagery, comments, jokes, or unwelcome sexual attention. +- Verbal and text comments that reinforce social structures of domination related to gender, gender identity and expression, sexual orientation, ability, physical appearance, body size, race, age, religion, or work experience. ### Online Trolling -The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the added issue of online trolling. This is unacceptable, reports of such behaviour will be taken very seriously, and perpetrators will be excluded from activities immediately. +The majority of nf-core interactions and events are held online. Unfortunately, holding events online comes with the risk of online trolling. This is unacceptable — reports of such behaviour will be taken very seriously and perpetrators will be excluded from activities immediately. -All community members are required to ask members of the group they are working within for explicit consent prior to taking screenshots of individuals during video calls. +All community members are **required** to ask members of the group they are working with for explicit consent prior to taking screenshots of individuals during video calls. -## Procedures for Reporting CoC violations +## Procedures for reporting CoC violations If someone makes you feel uncomfortable through their behaviours or actions, report it as soon as possible. -You can reach out to members of the [nf-core core team](https://nf-co.re/about) and they will forward your concerns to the safety officer(s). +You can reach out to members of the Safety Team (Saba Nafees, Cris Tuñí, and Michael Heuer) on Slack. Alternatively, contact a member of the nf-core core team [nf-core core team](https://nf-co.re/about), and they will forward your concerns to the Safety Team. + +Issues directly concerning members of the Core Team or the Safety Team will be dealt with by other members of the core team and the safety manager — possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson and details will be shared in due course. + +All reports will be handled with the utmost discretion and confidentiality. + +You can also report any CoC violations to safety [at] nf-co [dot] re. In your email report, please do your best to include: + +- Your contact information. +- Identifying information (e.g. names, nicknames, pseudonyms) of the participant who has violated the Code of Conduct. +- The behaviour that was in violation and the circumstances surrounding the incident. +- The approximate time of the behaviour (if different than the time the report was made). +- Other people involved in the incident, if applicable. +- If you believe the incident is ongoing. +- If there is a publicly available record (e.g. mailing list record, a screenshot). +- Any additional information. + +After you file a report, one or more members of our Safety Team will contact you to follow up on your report. + +## Who will read and handle reports + +All reports will be read and handled by the members of the Safety Team at nf-core. + +If members of the Safety Team are deemed to have a conflict of interest with a report, they will be required to recuse themselves as per our Code of Conduct and will not have access to any follow-ups. + +To keep this first report confidential from any of the Safety Team members, please submit your first report by direct messaging on Slack/direct email to any of the nf-core members you are comfortable disclosing the information to, and be explicit about which member(s) you do not consent to sharing the information with. + +## Reviewing reports + +After receiving the report, members of the Safety Team will review the incident report to determine whether immediate action is required, for example, whether there is immediate threat to participants’ safety. + +The Safety Team, in consultation with members of the nf-core core team, will assess the information to determine whether the report constitutes a Code of Conduct violation, for them to decide on a course of action. + +In the case of insufficient information, one or more members of the Safety Team may contact the reporter, the reportee, or any other attendees to obtain more information. -Issues directly concerning members of the core team will be dealt with by other members of the core team and the safety manager, and possible conflicts of interest will be taken into account. nf-core is also in discussions about having an ombudsperson, and details will be shared in due course. +Once additional information is gathered, the Safety Team will collectively review and decide on the best course of action to take, if any. The Safety Team reserves the right to not act on a report. -All reports will be handled with utmost discretion and confidentially. +## Confidentiality + +All reports, and any additional information included, are only shared with the team of safety officers (and possibly members of the core team, in case the safety officer is in violation of the CoC). We will respect confidentiality requests for the purpose of protecting victims of abuse. + +We will not name harassment victims, beyond discussions between the safety officer and members of the nf-core team, without the explicit consent of the individuals involved. + +## Enforcement + +Actions taken by the nf-core’s Safety Team may include, but are not limited to: + +- Asking anyone to stop a behaviour. +- Asking anyone to leave the event and online spaces either temporarily, for the remainder of the event, or permanently. +- Removing access to the gather.town and Slack, either temporarily or permanently. +- Communicating to all participants to reinforce our expectations for conduct and remind what is unacceptable behaviour; this may be public for practical reasons. +- Communicating to all participants that an incident has taken place and how we will act or have acted — this may be for the purpose of letting event participants know we are aware of and dealing with the incident. +- Banning anyone from participating in nf-core-managed spaces, future events, and activities, either temporarily or permanently. +- No action. ## Attribution and Acknowledgements @@ -106,6 +161,22 @@ All reports will be handled with utmost discretion and confidentially. ## Changelog -### v1.0 - March 12th, 2021 +### v1.4 - February 8th, 2022 + +- Included a new member of the Safety Team. Corrected a typographical error in the text. + +### v1.3 - December 10th, 2021 + +- Added a statement that the CoC applies to nf-core gather.town workspaces. Corrected typographical errors in the text. + +### v1.2 - November 12th, 2021 + +- Removed information specific to reporting CoC violations at the Hackathon in October 2021. + +### v1.1 - October 14th, 2021 + +- Updated with names of new Safety Officers and specific information for the hackathon in October 2021. + +### v1.0 - March 15th, 2021 - Complete rewrite from original [Contributor Covenant](http://contributor-covenant.org/) CoC. diff --git a/README.md b/README.md index e5953300..fcc23e20 100644 --- a/README.md +++ b/README.md @@ -1,6 +1,7 @@ # ![nf-core/chipseq](docs/images/nf-core-chipseq_logo_light.png#gh-light-mode-only) ![nf-core/chipseq](docs/images/nf-core-chipseq_logo_dark.png#gh-dark-mode-only) -[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![GitHub Actions CI Status](https://github.com/nf-core/chipseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/chipseq/actions?query=workflow%3A%22nf-core+CI%22) +[![GitHub Actions Linting Status](https://github.com/nf-core/chipseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/chipseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) @@ -29,10 +30,11 @@ ## Usage -> **Note** -> If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how -> to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) -> with `-profile test` before running the workflow on actual data. +:::note +If you are new to Nextflow and nf-core, please refer to [this page](https://nf-co.re/docs/usage/installation) on how +to set-up Nextflow. Make sure to [test your setup](https://nf-co.re/docs/usage/introduction#how-to-run-a-pipeline) +with `-profile test` before running the workflow on actual data. +::: - + diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index bd44a3a2..f2146258 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,5 +1,5 @@ report_comment: > - This report has been generated by the nf-core/chipseq + This report has been generated by the nf-core/chipseq analysis pipeline. For information about how to interpret these results, please see the documentation. report_section_order: diff --git a/assets/slackreport.json b/assets/slackreport.json index 5538e37c..8058c9bf 100644 --- a/assets/slackreport.json +++ b/assets/slackreport.json @@ -3,7 +3,7 @@ { "fallback": "Plain-text summary of the attachment.", "color": "<% if (success) { %>good<% } else { %>danger<%} %>", - "author_name": "nf-core/chipseq v${version} - ${runName}", + "author_name": "nf-core/chipseq ${version} - ${runName}", "author_icon": "https://www.nextflow.io/docs/latest/_static/favicon.ico", "text": "<% if (success) { %>Pipeline completed successfully!<% } else { %>Pipeline completed with errors<% } %>", "fields": [ diff --git a/conf/modules.config b/conf/modules.config index 39e81386..d91c6aba 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -39,7 +39,7 @@ process { } withName: 'MULTIQC' { - ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' + ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ path: { "${params.outdir}/multiqc" }, mode: params.publish_dir_mode, diff --git a/docs/usage.md b/docs/usage.md index 81181cbb..09080185 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -20,7 +20,7 @@ You will need to create a samplesheet with information about the samples you wou The `sample` identifiers have to be the same when you have re-sequenced the same sample more than once e.g. to increase sequencing depth. The pipeline will concatenate the raw reads before performing any downstream analysis. Below is an example for the same sample sequenced across 3 lanes: -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP1,AEG588A1_S1_L003_R1_001.fastq.gz,AEG588A1_S1_L003_R2_001.fastq.gz @@ -33,7 +33,7 @@ The pipeline will auto-detect whether a sample is single- or paired-end using th A final samplesheet file consisting of both single- and paired-end data may look something like the one below. This is for 6 samples, where `TREATMENT_REP3` has been sequenced twice. -```console +```csv title="samplesheet.csv" sample,fastq_1,fastq_2 CONTROL_REP1,AEG588A1_S1_L002_R1_001.fastq.gz,AEG588A1_S1_L002_R2_001.fastq.gz CONTROL_REP2,AEG588A2_S2_L002_R1_001.fastq.gz,AEG588A2_S2_L002_R2_001.fastq.gz diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy index 01b8653d..e248e4c3 100755 --- a/lib/NfcoreTemplate.groovy +++ b/lib/NfcoreTemplate.groovy @@ -4,6 +4,7 @@ import org.yaml.snakeyaml.Yaml import groovy.json.JsonOutput +import nextflow.extension.FilesEx class NfcoreTemplate { @@ -141,12 +142,14 @@ class NfcoreTemplate { try { if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } [ 'sendmail', '-t' ].execute() << sendmail_html log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" } catch (all) { // Catch failures and try with plaintext def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report.size() <= max_multiqc_email_size.toBytes() ) { + if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { mail_cmd += [ '-A', mqc_report ] } mail_cmd.execute() << email_html @@ -155,14 +158,16 @@ class NfcoreTemplate { } // Write summary e-mail HTML to a file - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def output_hf = new File(output_d, "pipeline_report.html") + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") output_hf.withWriter { w -> w << email_html } - def output_tf = new File(output_d, "pipeline_report.txt") + FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() } // @@ -227,15 +232,14 @@ class NfcoreTemplate { // Dump pipeline parameters in a json file // public static void dump_parameters(workflow, params) { - def output_d = new File("${params.outdir}/pipeline_info/") - if (!output_d.exists()) { - output_d.mkdirs() - } - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def output_pf = new File(output_d, "params_${timestamp}.json") + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") def jsonStr = JsonOutput.toJson(params) - output_pf.text = JsonOutput.prettyPrint(jsonStr) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() } // diff --git a/modules.json b/modules.json index d0cd2499..893d6c2c 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "bd8092b67b5103bdd52e300f75889442275c3117", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "4ab13872435962dadc239979554d13709e20bf29", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml new file mode 100644 index 00000000..f0c63f69 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -0,0 +1,7 @@ +name: custom_dumpsoftwareversions +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.17 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index ebc87273..7685b33c 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -2,10 +2,10 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { label 'process_single' // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : + 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml index c32657de..5f15a5fd 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/meta.yml @@ -1,4 +1,4 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: custom_dumpsoftwareversions description: Custom module used to dump software versions within the nf-core pipeline template keywords: @@ -16,7 +16,6 @@ input: type: file description: YML file containing software versions pattern: "*.yml" - output: - yml: type: file @@ -30,7 +29,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test new file mode 100644 index 00000000..eec1db10 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -0,0 +1,38 @@ +nextflow_process { + + name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" + script "../main.nf" + process "CUSTOM_DUMPSOFTWAREVERSIONS" + tag "modules" + tag "modules_nfcore" + tag "custom" + tag "dumpsoftwareversions" + tag "custom/dumpsoftwareversions" + + test("Should run without failures") { + when { + process { + """ + def tool1_version = ''' + TOOL1: + tool1: 0.11.9 + '''.stripIndent() + + def tool2_version = ''' + TOOL2: + tool2: 1.9 + '''.stripIndent() + + input[0] = Channel.of(tool1_version, tool2_version).collectFile() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap new file mode 100644 index 00000000..4274ed57 --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -0,0 +1,27 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ], + "1": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "2": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "mqc_yml": [ + "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" + ], + "versions": [ + "versions.yml:md5,3843ac526e762117eedf8825b40683df" + ], + "yml": [ + "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" + ] + } + ], + "timestamp": "2023-11-03T14:43:22.157011" + } +} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml new file mode 100644 index 00000000..405aa24a --- /dev/null +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml @@ -0,0 +1,2 @@ +custom/dumpsoftwareversions: + - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/environment.yml b/modules/nf-core/fastqc/environment.yml new file mode 100644 index 00000000..1787b38a --- /dev/null +++ b/modules/nf-core/fastqc/environment.yml @@ -0,0 +1,7 @@ +name: fastqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::fastqc=0.12.1 diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 249f9064..9e19a74c 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -2,10 +2,10 @@ process FASTQC { tag "$meta.id" label 'process_medium' - conda "bioconda::fastqc=0.11.9" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/fastqc:0.11.9--0' : - 'biocontainers/fastqc:0.11.9--0' }" + 'https://depot.galaxyproject.org/singularity/fastqc:0.12.1--hdfd78af_0' : + 'biocontainers/fastqc:0.12.1--hdfd78af_0' }" input: tuple val(meta), path(reads) @@ -37,7 +37,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ @@ -49,7 +49,7 @@ process FASTQC { cat <<-END_VERSIONS > versions.yml "${task.process}": - fastqc: \$( fastqc --version | sed -e "s/FastQC v//g" ) + fastqc: \$( fastqc --version | sed '/FastQC v/!d; s/.*v//' ) END_VERSIONS """ } diff --git a/modules/nf-core/fastqc/meta.yml b/modules/nf-core/fastqc/meta.yml index 4da5bb5a..ee5507e0 100644 --- a/modules/nf-core/fastqc/meta.yml +++ b/modules/nf-core/fastqc/meta.yml @@ -50,3 +50,8 @@ authors: - "@grst" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@grst" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test new file mode 100644 index 00000000..b9e8f926 --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -0,0 +1,109 @@ +nextflow_process { + + name "Test Process FASTQC" + script "../main.nf" + process "FASTQC" + tag "modules" + tag "modules_nfcore" + tag "fastqc" + + test("Single-Read") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id: 'test', single_end:true ], + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. + // looks like this:
    Mon 2 Oct 2023
    test.gz
    + // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 + { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, + { assert path(process.out.html.get(0).get(1)).getText().contains("File typeConventional base calls") }, + { assert snapshot(process.out.versions).match("versions") }, + { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + ) + } + } +// TODO +// // +// // Test with paired-end data +// // +// workflow test_fastqc_paired_end { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with interleaved data +// // +// workflow test_fastqc_interleaved { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with bam data +// // +// workflow test_fastqc_bam { +// input = [ +// [id: 'test', single_end: false], // meta map +// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with multiple samples +// // +// workflow test_fastqc_multiple { +// input = [ +// [id: 'test', single_end: false], // meta map +// [ +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), +// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) +// ] +// ] + +// FASTQC ( input ) +// } + +// // +// // Test with custom prefix +// // +// workflow test_fastqc_custom_prefix { +// input = [ +// [ id:'mysample', single_end:true ], // meta map +// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) +// ] + +// FASTQC ( input ) +// } +} diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap new file mode 100644 index 00000000..636a32ce --- /dev/null +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2023-10-09T23:40:54+0000" + } +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/tags.yml b/modules/nf-core/fastqc/tests/tags.yml new file mode 100644 index 00000000..7834294b --- /dev/null +++ b/modules/nf-core/fastqc/tests/tags.yml @@ -0,0 +1,2 @@ +fastqc: + - modules/nf-core/fastqc/** diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml new file mode 100644 index 00000000..bc0bdb5b --- /dev/null +++ b/modules/nf-core/multiqc/environment.yml @@ -0,0 +1,7 @@ +name: multiqc +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::multiqc=1.18 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1fc387be..00cc48d2 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -1,10 +1,10 @@ process MULTIQC { label 'process_single' - conda "bioconda::multiqc=1.14" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.14--pyhdfd78af_0' : - 'biocontainers/multiqc:1.14--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : + 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -25,12 +25,14 @@ process MULTIQC { def args = task.ext.args ?: '' def config = multiqc_config ? "--config $multiqc_config" : '' def extra_config = extra_multiqc_config ? "--config $extra_multiqc_config" : '' + def logo = multiqc_logo ? /--cl-config 'custom_logo: "${multiqc_logo}"'/ : '' """ multiqc \\ --force \\ $args \\ $config \\ $extra_config \\ + $logo \\ . cat <<-END_VERSIONS > versions.yml diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f93b5ee5..f1aa660e 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,5 +1,5 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json -name: MultiQC +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: - QC @@ -13,7 +13,6 @@ tools: homepage: https://multiqc.info/ documentation: https://multiqc.info/docs/ licence: ["GPL-3.0-or-later"] - input: - multiqc_files: type: file @@ -31,7 +30,6 @@ input: type: file description: Optional logo file for MultiQC pattern: "*.{png}" - output: - report: type: file @@ -54,3 +52,8 @@ authors: - "@bunop" - "@drpatelh" - "@jfy133" +maintainers: + - "@abhi18av" + - "@bunop" + - "@drpatelh" + - "@jfy133" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test new file mode 100644 index 00000000..c2dad217 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -0,0 +1,63 @@ +nextflow_process { + + name "Test Process MULTIQC" + script "../main.nf" + process "MULTIQC" + tag "modules" + tag "modules_nfcore" + tag "multiqc" + + test("MULTIQC: FASTQC") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } + + test("MULTIQC: FASTQC and a config file") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.report.get(0)).exists() }, + { assert path(process.out.data.get(0)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + ) + } + + } +} diff --git a/modules/nf-core/multiqc/tests/tags.yml b/modules/nf-core/multiqc/tests/tags.yml new file mode 100644 index 00000000..bea6c0d3 --- /dev/null +++ b/modules/nf-core/multiqc/tests/tags.yml @@ -0,0 +1,2 @@ +multiqc: + - modules/nf-core/multiqc/** diff --git a/nextflow.config b/nextflow.config index f73726af..dd2e16bb 100644 --- a/nextflow.config +++ b/nextflow.config @@ -14,7 +14,7 @@ params { input = null // References genome = null - igenomes_base = 's3://ngi-igenomes/igenomes' + igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false @@ -82,6 +82,7 @@ profiles { dumpHashes = true process.beforeScript = 'echo $HOSTNAME' cleanup = false + nextflow.enable.configProcessNamesValidation = true } conda { conda.enabled = true @@ -104,13 +105,13 @@ profiles { } docker { docker.enabled = true - docker.userEmulation = true conda.enabled = false singularity.enabled = false podman.enabled = false shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false + runOptions = '-u $(id -u):$(id -g)' } arm { docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' @@ -181,7 +182,7 @@ singularity.registry = 'quay.io' // Nextflow plugins plugins { - id 'nf-validation' // Validation of pipeline parameters and creation of an input channel from a sample sheet + id 'nf-validation@1.1.3' // Validation of pipeline parameters and creation of an input channel from a sample sheet } // Load igenomes.config if required @@ -204,6 +205,9 @@ env { // Capture exit codes from upstream processes when piping process.shell = ['/bin/bash', '-euo', 'pipefail'] +// Disable process selector warnings by default. Use debug profile to enable warnings. +nextflow.enable.configProcessNamesValidation = false + def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') timeline { enabled = true From 3d374433876f193fa289306608790141baff9686 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 20 Dec 2023 15:56:29 +0000 Subject: [PATCH 129/201] Template update for nf-core/tools version 2.11.1 --- .../{release-announcments.yml => release-announcements.yml} | 0 nextflow.config | 4 ++-- 2 files changed, 2 insertions(+), 2 deletions(-) rename .github/workflows/{release-announcments.yml => release-announcements.yml} (100%) diff --git a/.github/workflows/release-announcments.yml b/.github/workflows/release-announcements.yml similarity index 100% rename from .github/workflows/release-announcments.yml rename to .github/workflows/release-announcements.yml diff --git a/nextflow.config b/nextflow.config index dd2e16bb..e4367873 100644 --- a/nextflow.config +++ b/nextflow.config @@ -111,10 +111,10 @@ profiles { shifter.enabled = false charliecloud.enabled = false apptainer.enabled = false - runOptions = '-u $(id -u):$(id -g)' + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { singularity.enabled = true From 220d815cb365f1c8d62bde2ef9897fe4626e4007 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 14:49:47 +0000 Subject: [PATCH 130/201] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-chipseq_logo_light.png | Bin 73788 -> 77165 bytes docs/images/nf-core-chipseq_logo_dark.png | Bin 73955 -> 27741 bytes docs/images/nf-core-chipseq_logo_light.png | Bin 73788 -> 23897 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 3 +- pyproject.toml | 17 +- workflows/chipseq.nf | 7 + 36 files changed, 490 insertions(+), 221 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b31907..9b990088 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 500a2167..1aacf8b4 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 6b16318d..13edafbc 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index cc0e5a11..d98ab5f1 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90ec..e37cfda5 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 00000000..8611458a --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index c1499ef1..d7e08c32 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/chipseq/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e4..81cd098e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f..147bcd10 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad33927..21ac3f06 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf72695..363d5b1d 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb9..af57081f 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/README.md b/README.md index 87f23f75..bd964cef 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/chipseq](docs/images/nf-core-chipseq_logo_light.png#gh-light-mode-only) ![nf-core/chipseq](docs/images/nf-core-chipseq_logo_dark.png#gh-dark-mode-only) - +

    + + + nf-core/chipseq + +

    [![GitHub Actions CI Status](https://github.com/nf-core/chipseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/chipseq/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/chipseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/chipseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index 5a4548bb..4398d399 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

    nf-core/chipseq v${version}

    +

    nf-core/chipseq ${version}

    Run Name: $runName

    <% if (!success){ diff --git a/assets/email_template.txt b/assets/email_template.txt index e1b78526..5d158118 100644 --- a/assets/email_template.txt +++ b/assets/email_template.txt @@ -4,7 +4,7 @@ |\\ | |__ __ / ` / \\ |__) |__ } { | \\| | \\__, \\__/ | \\ |___ \\`-._,-`-, `._,._,' - nf-core/chipseq v${version} + nf-core/chipseq ${version} ---------------------------------------------------- Run Name: $runName diff --git a/assets/nf-core-chipseq_logo_light.png b/assets/nf-core-chipseq_logo_light.png index 51a83b3d789d5852acc2bb044650b13785c6bf41..ef93395ecd5bbb73aa3eeca0359b65461ce3389e 100644 GIT binary patch literal 77165 zcmeEui93|*|NktQ%2p{POG2_GTh>fbF$vKkWvL`7S+eglWvLVyq^!vx5wgoR)sd*| zWoJr4wy}@>_kPs*ocb4j*Y~ijuVhOY*KysvQ|q$y*J-S zk72oMpYe0q?VAbaq#*9EMu$IdcoXp^so>_-$xz2=@*S~&`he~jv*V1qmOg7gB~|ku z4iN>q9{kdyZ|YpwtFN7@xuSZ(Q#H%X?NoYKwa=aXj}v2~+N64J89ds7jyopzTE&lL zTX=gIP6(U()S#j$694J$cS-q-%;cBHV%V6wo@O1Lq5Km$NZ4lj<2c zwqe?icigw3j2(aYEkjNIUZPcw=4bC$%q+7b?p1P^!$01``3%CuY^87 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- public static void initialise(workflow, params, log) { + public static void initialise(workflow, params, log, args) { // Print workflow version and exit on --version if (params.version) { @@ -35,6 +35,8 @@ class WorkflowMain { // Check that a -profile or Nextflow config has been provided to run the pipeline NfcoreTemplate.checkConfigProvided(workflow, log) + // Check that the profile doesn't contain spaces and doesn't end with a trailing comma + checkProfile(workflow.profile, args, log) // Check that conda channels are set-up correctly if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { @@ -60,4 +62,16 @@ class WorkflowMain { } return null } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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b/modules.json index 893d6c2c..d4a463ff 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..9b3272bc 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..f2187611 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f926..1f21c664 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
    Mon 2 Oct 2023
    test.gz
    // https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32ce..5d624bb8 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5b..7625b752 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d2..1b9f7c43 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660e..45a9bc35 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad217..d0438eda 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 00000000..d37e7304 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index e4367873..59e48f09 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,7 @@ try { } // Load nf-core/chipseq custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/chipseq.config" // } catch (Exception e) { @@ -91,6 +91,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6..7d08e1c8 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 1f747dc3..3bac800f 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From a5f02598f501cf21d2dc0dd1c2eb051beb355d93 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 1 Feb 2024 15:07:04 +0100 Subject: [PATCH 131/201] Fix lint --- .nf-core.yml | 6 ++++++ nextflow_schema.json | 2 +- 2 files changed, 7 insertions(+), 1 deletion(-) diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc81..e8247adb 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,7 @@ repository_type: pipeline + +lint: + nextflow_config: + - config_defaults: + - params.bamtools_filter_se_config + - params.bamtools_filter_pe_config diff --git a/nextflow_schema.json b/nextflow_schema.json index 1cc86686..c05ca671 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -163,7 +163,7 @@ "type": "string", "format": "directory-path", "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", + "default": "s3://ngi-igenomes/igenomes/", "fa_icon": "fas fa-cloud-download-alt", "hidden": true }, From 2efa8faf5cd5f6a93e65006abe2db01bd1d39446 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 1 Feb 2024 15:53:41 +0100 Subject: [PATCH 132/201] fix linting --- modules.json | 201 +++++++++++++----- .../nf-core/bam_markduplicates_picard/main.nf | 12 +- .../bam_markduplicates_picard/meta.yml | 8 +- .../tests/main.nf.test | 93 ++++++++ .../tests/main.nf.test.snap | 22 ++ .../bam_markduplicates_picard/tests/tags.yml | 2 + .../nf-core/bam_sort_stats_samtools/meta.yml | 6 +- .../tests/main.nf.test | 82 +++++++ .../tests/main.nf.test.snap | 86 ++++++++ .../bam_sort_stats_samtools/tests/tags.yml | 2 + .../nf-core/bam_stats_samtools/main.nf | 2 +- .../nf-core/bam_stats_samtools/meta.yml | 4 +- .../bam_stats_samtools/tests/main.nf.test | 108 ++++++++++ .../tests/main.nf.test.snap | 128 +++++++++++ .../nf-core/bam_stats_samtools/tests/tags.yml | 2 + .../nf-core/fastq_align_bowtie2/main.nf | 8 +- .../nf-core/fastq_align_bowtie2/meta.yml | 10 +- subworkflows/nf-core/fastq_align_bwa/main.nf | 2 +- subworkflows/nf-core/fastq_align_bwa/meta.yml | 13 +- .../nf-core/fastq_align_chromap/main.nf | 2 +- .../nf-core/fastq_align_chromap/meta.yml | 5 +- .../fastq_fastqc_umitools_trimgalore/meta.yml | 12 +- 22 files changed, 726 insertions(+), 84 deletions(-) create mode 100644 subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test create mode 100644 subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test.snap create mode 100644 subworkflows/nf-core/bam_markduplicates_picard/tests/tags.yml create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap create mode 100644 subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap create mode 100644 subworkflows/nf-core/bam_stats_samtools/tests/tags.yml diff --git a/modules.json b/modules.json index 758b63a5..99214bc7 100644 --- a/modules.json +++ b/modules.json @@ -8,177 +8,259 @@ "bowtie2/align": { "branch": "master", "git_sha": "fe54581f8bed20e4c4a51c616c93fd3379d89820", - "installed_by": ["modules", "fastq_align_bowtie2"] + "installed_by": [ + "modules", + "fastq_align_bowtie2" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", - "installed_by": ["modules", "fastq_align_bwa"] + "installed_by": [ + "modules", + "fastq_align_bwa" + ] }, "chromap/chromap": { "branch": "master", "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", - "installed_by": ["modules", "fastq_align_chromap"] + "installed_by": [ + "modules", + "fastq_align_chromap" + ] }, "chromap/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/getchromsizes": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/computematrix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "fastqc": { "branch": "master", - "git_sha": "9a4517e720bc812e95b56d23d15a1653b6db4f53", - "installed_by": ["modules", "fastq_fastqc_umitools_trimgalore"] + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "gffread": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "macs2/callpeak": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "phantompeakqualtools": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/markduplicates": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": ["modules", "bam_markduplicates_picard"] + "installed_by": [ + "modules", + "bam_markduplicates_picard" + ] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "preseq/lcextrap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/flagstat": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules", "bam_stats_samtools"] + "installed_by": [ + "modules", + "bam_stats_samtools" + ] }, "samtools/idxstats": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules", "bam_stats_samtools"] + "installed_by": [ + "modules", + "bam_stats_samtools" + ] }, "samtools/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules", "bam_markduplicates_picard", "bam_sort_stats_samtools"] + "installed_by": [ + "modules", + "bam_markduplicates_picard", + "bam_sort_stats_samtools" + ] }, "samtools/sort": { "branch": "master", "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9", - "installed_by": ["modules", "bam_sort_stats_samtools"] + "installed_by": [ + "modules", + "bam_sort_stats_samtools" + ] }, "samtools/stats": { "branch": "master", "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": ["modules", "bam_stats_samtools"] + "installed_by": [ + "modules", + "bam_stats_samtools" + ] }, "subread/featurecounts": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "trimgalore": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "umitools/extract": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["fastq_fastqc_umitools_trimgalore"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore" + ] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "untarfiles": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -186,41 +268,58 @@ "nf-core": { "bam_markduplicates_picard": { "branch": "master", - "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", - "installed_by": ["subworkflows"] + "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", + "installed_by": [ + "subworkflows" + ] }, "bam_sort_stats_samtools": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": ["fastq_align_bowtie2", "fastq_align_chromap", "fastq_align_bwa"] + "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", + "installed_by": [ + "fastq_align_bowtie2", + "fastq_align_bwa", + "fastq_align_chromap" + ] }, "bam_stats_samtools": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": ["bam_sort_stats_samtools", "bam_markduplicates_picard"] + "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", + "installed_by": [ + "bam_markduplicates_picard", + "bam_sort_stats_samtools" + ] }, "fastq_align_bowtie2": { "branch": "master", - "git_sha": "ac75f79157ecc64283a2b3a559f1ba90bc0f2259", - "installed_by": ["subworkflows"] + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": [ + "subworkflows" + ] }, "fastq_align_bwa": { "branch": "master", - "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", - "installed_by": ["subworkflows"] + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": [ + "subworkflows" + ] }, "fastq_align_chromap": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", - "installed_by": ["subworkflows"] + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": [ + "subworkflows" + ] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", - "git_sha": "a9784afdd5dcda23b84e64db75dc591065d64653", - "installed_by": ["subworkflows"] + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": [ + "subworkflows" + ] } } } } } -} +} \ No newline at end of file diff --git a/subworkflows/nf-core/bam_markduplicates_picard/main.nf b/subworkflows/nf-core/bam_markduplicates_picard/main.nf index 3725ea95..de8130fb 100644 --- a/subworkflows/nf-core/bam_markduplicates_picard/main.nf +++ b/subworkflows/nf-core/bam_markduplicates_picard/main.nf @@ -17,7 +17,7 @@ workflow BAM_MARKDUPLICATES_PICARD { ch_versions = Channel.empty() - PICARD_MARKDUPLICATES ( ch_bam, ch_fasta.map { [ [:], it ] }, ch_fai.map { [ [:], it ] } ) + PICARD_MARKDUPLICATES ( ch_bam, ch_fasta, ch_fai ) ch_versions = ch_versions.mix(PICARD_MARKDUPLICATES.out.versions.first()) SAMTOOLS_INDEX ( PICARD_MARKDUPLICATES.out.bam ) @@ -26,13 +26,9 @@ workflow BAM_MARKDUPLICATES_PICARD { ch_bam_bai = PICARD_MARKDUPLICATES.out.bam .join(SAMTOOLS_INDEX.out.bai, by: [0], remainder: true) .join(SAMTOOLS_INDEX.out.csi, by: [0], remainder: true) - .map { - meta, bam, bai, csi -> - if (bai) { - [ meta, bam, bai ] - } else { - [ meta, bam, csi ] - } + .map{meta, bam, bai, csi -> + if (bai) [ meta, bam, bai ] + else [ meta, bam, csi ] } BAM_STATS_SAMTOOLS ( ch_bam_bai, ch_fasta ) diff --git a/subworkflows/nf-core/bam_markduplicates_picard/meta.yml b/subworkflows/nf-core/bam_markduplicates_picard/meta.yml index d5e71609..fe63068e 100644 --- a/subworkflows/nf-core/bam_markduplicates_picard/meta.yml +++ b/subworkflows/nf-core/bam_markduplicates_picard/meta.yml @@ -6,14 +6,13 @@ keywords: - bam - sam - cram - -modules: +components: - picard/markduplicates - samtools/index - samtools/stats - samtools/idxstats - samtools/flagstat - + - bam_stats_samtools input: - ch_bam: description: | @@ -59,3 +58,6 @@ output: authors: - "@dmarron" - "@drpatelh" +maintainers: + - "@dmarron" + - "@drpatelh" diff --git a/subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test b/subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test new file mode 100644 index 00000000..d8d24290 --- /dev/null +++ b/subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test @@ -0,0 +1,93 @@ +nextflow_workflow { + + name "Test Workflow BAM_MARKDUPLICATES_PICARD" + script "../main.nf" + workflow "BAM_MARKDUPLICATES_PICARD" + + tag "picard" + tag "picard/markduplicates" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "bam_markduplicates_picard" + tag "subworkflows/bam_markduplicates_picard" + tag "subworkflows/bam_stats_samtools" + tag "bam_stats_samtools" + tag "samtools" + tag "samtools/flagstat" + tag "samtools/idxstats" + tag "samtools/index" + tag "samtools/stats" + + test("sarscov2 - bam") { + + when { + workflow { + """ + input[0] = Channel.of([ + [ id:'test', single_end: false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot( + path(workflow.out.bam[0][1]), + path(workflow.out.bai[0][1]), + path(workflow.out.flagstat[0][1]), + path(workflow.out.idxstats[0][1]), + path(workflow.out.stats[0][1]), + ).match("sarscov2 - bam") }, + { assert path(workflow.out.metrics.get(0).get(1)).getText().contains("97") } + ) + } + } + + test("homo_sapiens - cram") { + + when { + workflow { + """ + input[0] = Channel.of([ + [ id:'test' ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot( + path(workflow.out.bam[0][1]), + path(workflow.out.bai[0][1]), + path(workflow.out.flagstat[0][1]), + path(workflow.out.idxstats[0][1]), + path(workflow.out.stats[0][1]), + ).match("homo_sapiens - cram") }, + { assert path(workflow.out.metrics.get(0).get(1)).getText().contains("0.999986") } + ) + } + } + +} diff --git a/subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test.snap b/subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test.snap new file mode 100644 index 00000000..a208d101 --- /dev/null +++ b/subworkflows/nf-core/bam_markduplicates_picard/tests/main.nf.test.snap @@ -0,0 +1,22 @@ +{ + "homo_sapiens - cram": { + "content": [ + "test.bam:md5,6641dc05efa8384a061f378d86d922cd", + "test.bam.bai:md5,c41c60d8a94adebe53b6df80b6e90d38", + "test.flagstat:md5,93b0ef463df947ede1f42ff60396c34d", + "test.idxstats:md5,e179601fa7b8ebce81ac3765206f6c15", + "test.stats:md5,0035ac8900d85e9a790f4c1f48b76947" + ], + "timestamp": "2023-12-05T17:45:12.484869" + }, + "sarscov2 - bam": { + "content": [ + "test.bam:md5,3091fe6ba1b7530f382fe40b9fd8f45b", + "test.bam.bai:md5,4d3ae8d013444b55e17aa0149a2ab404", + "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783", + "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2", + "test.stats:md5,e32e7e49dce1fbe327a89e0fb7bc01b1" + ], + "timestamp": "2023-12-05T17:43:58.582652" + } +} diff --git a/subworkflows/nf-core/bam_markduplicates_picard/tests/tags.yml b/subworkflows/nf-core/bam_markduplicates_picard/tests/tags.yml new file mode 100644 index 00000000..10b85270 --- /dev/null +++ b/subworkflows/nf-core/bam_markduplicates_picard/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/bam_markduplicates_picard: + - subworkflows/nf-core/bam_markduplicates_picard/** diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml index 8dfbd58d..e01f9ccf 100644 --- a/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml +++ b/subworkflows/nf-core/bam_sort_stats_samtools/meta.yml @@ -6,12 +6,13 @@ keywords: - bam - sam - cram -modules: +components: - samtools/sort - samtools/index - samtools/stats - samtools/idxstats - samtools/flagstat + - bam_stats_samtools input: - meta: type: map @@ -64,3 +65,6 @@ output: authors: - "@drpatelh" - "@ewels" +maintainers: + - "@drpatelh" + - "@ewels" diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test new file mode 100644 index 00000000..75b5b934 --- /dev/null +++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test @@ -0,0 +1,82 @@ +nextflow_workflow { + + name "Test Workflow BAM_SORT_STATS_SAMTOOLS" + script "../main.nf" + workflow "BAM_SORT_STATS_SAMTOOLS" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "subworkflows/bam_sort_stats_samtools" + tag "bam_sort_stats_samtools" + tag "subworkflows/bam_stats_samtools" + tag "bam_stats_samtools" + tag "samtools" + tag "samtools/index" + tag "samtools/sort" + tag "samtools/stats" + tag "samtools/idxstats" + tag "samtools/flagstat" + + test("test_bam_sort_stats_samtools_single_end") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.bam', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert workflow.out.bam.get(0).get(1) ==~ ".*.bam"}, + { assert workflow.out.bai.get(0).get(1) ==~ ".*.bai"}, + { assert snapshot(workflow.out.stats).match("test_bam_sort_stats_samtools_single_end_stats") }, + { assert snapshot(workflow.out.flagstat).match("test_bam_sort_stats_samtools_single_end_flagstats") }, + { assert snapshot(workflow.out.idxstats).match("test_bam_sort_stats_samtools_single_end_idxstats") } + ) + } + } + + test("test_bam_sort_stats_samtools_paired_end") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert workflow.out.bam.get(0).get(1) ==~ ".*.bam"}, + { assert workflow.out.bai.get(0).get(1) ==~ ".*.bai"}, + { assert snapshot(workflow.out.stats).match("test_bam_sort_stats_samtools_paired_end_stats") }, + { assert snapshot(workflow.out.flagstat).match("test_bam_sort_stats_samtools_paired_end_flagstats") }, + { assert snapshot(workflow.out.idxstats).match("test_bam_sort_stats_samtools_paired_end_idxstats") } + ) + } + } +} diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap new file mode 100644 index 00000000..c159eef3 --- /dev/null +++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/main.nf.test.snap @@ -0,0 +1,86 @@ +{ + "test_bam_sort_stats_samtools_paired_end_flagstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783" + ] + ] + ], + "timestamp": "2023-10-22T20:25:03.687121177" + }, + "test_bam_sort_stats_samtools_paired_end_idxstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2" + ] + ] + ], + "timestamp": "2023-10-22T20:25:03.709648916" + }, + "test_bam_sort_stats_samtools_single_end_stats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,f281507081517414eb1a04b2d9c855b2" + ] + ] + ], + "timestamp": "2024-01-18T17:10:02.818694" + }, + "test_bam_sort_stats_samtools_paired_end_stats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,e32e7e49dce1fbe327a89e0fb7bc01b1" + ] + ] + ], + "timestamp": "2023-12-04T11:06:59.253905951" + }, + "test_bam_sort_stats_samtools_single_end_idxstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20" + ] + ] + ], + "timestamp": "2024-01-18T17:10:02.84631" + }, + "test_bam_sort_stats_samtools_single_end_flagstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53" + ] + ] + ], + "timestamp": "2024-01-18T17:10:02.829756" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml b/subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml new file mode 100644 index 00000000..30b69d6a --- /dev/null +++ b/subworkflows/nf-core/bam_sort_stats_samtools/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/bam_sort_stats_samtools: + - subworkflows/nf-core/bam_sort_stats_samtools/** diff --git a/subworkflows/nf-core/bam_stats_samtools/main.nf b/subworkflows/nf-core/bam_stats_samtools/main.nf index e80ce43a..44d4c010 100644 --- a/subworkflows/nf-core/bam_stats_samtools/main.nf +++ b/subworkflows/nf-core/bam_stats_samtools/main.nf @@ -14,7 +14,7 @@ workflow BAM_STATS_SAMTOOLS { main: ch_versions = Channel.empty() - SAMTOOLS_STATS ( ch_bam_bai, ch_fasta.map { [ [:], it ] } ) + SAMTOOLS_STATS ( ch_bam_bai, ch_fasta ) ch_versions = ch_versions.mix(SAMTOOLS_STATS.out.versions) SAMTOOLS_FLAGSTAT ( ch_bam_bai ) diff --git a/subworkflows/nf-core/bam_stats_samtools/meta.yml b/subworkflows/nf-core/bam_stats_samtools/meta.yml index b05086bc..809bf736 100644 --- a/subworkflows/nf-core/bam_stats_samtools/meta.yml +++ b/subworkflows/nf-core/bam_stats_samtools/meta.yml @@ -7,7 +7,7 @@ keywords: - bam - sam - cram -modules: +components: - samtools/stats - samtools/idxstats - samtools/flagstat @@ -39,3 +39,5 @@ output: Structure: [ path(versions.yml) ] authors: - "@drpatelh" +maintainers: + - "@drpatelh" diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test new file mode 100644 index 00000000..c8b21f28 --- /dev/null +++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test @@ -0,0 +1,108 @@ +nextflow_workflow { + + name "Test Workflow BAM_STATS_SAMTOOLS" + script "../main.nf" + workflow "BAM_STATS_SAMTOOLS" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "bam_stats_samtools" + tag "subworkflows/bam_stats_samtools" + tag "samtools" + tag "samtools/flagstat" + tag "samtools/idxstats" + tag "samtools/stats" + + test("test_bam_stats_samtools_single_end") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.sorted.bam.bai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out.stats).match("test_bam_stats_samtools_single_end_stats") }, + { assert snapshot(workflow.out.flagstat).match("test_bam_stats_samtools_single_end_flagstats") }, + { assert snapshot(workflow.out.idxstats).match("test_bam_stats_samtools_single_end_idxstats") } + ) + } + } + + test("test_bam_stats_samtools_paired_end") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out.stats).match("test_bam_stats_samtools_paired_end_stats") }, + { assert snapshot(workflow.out.flagstat).match("test_bam_stats_samtools_paired_end_flagstats") }, + { assert snapshot(workflow.out.idxstats).match("test_bam_stats_samtools_paired_end_idxstats") } + ) + } + } + + test("test_bam_stats_samtools_paired_end_cram") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram.crai', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert workflow.success}, + { assert snapshot(workflow.out.stats).match("test_bam_stats_samtools_paired_end_cram_stats") }, + { assert snapshot(workflow.out.flagstat).match("test_bam_stats_samtools_paired_end_cram_flagstats") }, + { assert snapshot(workflow.out.idxstats).match("test_bam_stats_samtools_paired_end_cram_idxstats") } + ) + } + } + +} diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap new file mode 100644 index 00000000..8bf0d379 --- /dev/null +++ b/subworkflows/nf-core/bam_stats_samtools/tests/main.nf.test.snap @@ -0,0 +1,128 @@ +{ + "test_bam_stats_samtools_paired_end_cram_flagstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,a53f3d26e2e9851f7d528442bbfe9781" + ] + ] + ], + "timestamp": "2023-11-06T09:31:26.194017574" + }, + "test_bam_stats_samtools_paired_end_stats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.stats:md5,49e2b43344ff92bc4c02463a58f7ba4a" + ] + ] + ], + "timestamp": "2024-01-18T17:17:27.704335" + }, + "test_bam_stats_samtools_paired_end_flagstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783" + ] + ] + ], + "timestamp": "2024-01-18T17:17:27.717482" + }, + "test_bam_stats_samtools_single_end_flagstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.flagstat:md5,2191911d72575a2358b08b1df64ccb53" + ] + ] + ], + "timestamp": "2023-11-06T09:26:10.340046381" + }, + "test_bam_stats_samtools_paired_end_cram_idxstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,e179601fa7b8ebce81ac3765206f6c15" + ] + ] + ], + "timestamp": "2023-11-06T09:31:26.207052003" + }, + "test_bam_stats_samtools_single_end_stats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.stats:md5,5a6667d97806e5002731e9cf23674fad" + ] + ] + ], + "timestamp": "2023-12-04T11:07:06.676820877" + }, + "test_bam_stats_samtools_paired_end_idxstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2" + ] + ] + ], + "timestamp": "2024-01-18T17:17:27.726719" + }, + "test_bam_stats_samtools_single_end_idxstats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.idxstats:md5,613e048487662c694aa4a2f73ca96a20" + ] + ] + ], + "timestamp": "2023-11-06T09:26:10.349439801" + }, + "test_bam_stats_samtools_paired_end_cram_stats": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,2cf2fe93596ee3d74f946097b204a629" + ] + ] + ], + "timestamp": "2023-12-04T11:07:22.30295557" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/bam_stats_samtools/tests/tags.yml b/subworkflows/nf-core/bam_stats_samtools/tests/tags.yml new file mode 100644 index 00000000..ec2f2d68 --- /dev/null +++ b/subworkflows/nf-core/bam_stats_samtools/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/bam_stats_samtools: + - subworkflows/nf-core/bam_stats_samtools/** diff --git a/subworkflows/nf-core/fastq_align_bowtie2/main.nf b/subworkflows/nf-core/fastq_align_bowtie2/main.nf index e77cb2c4..ba4420f7 100644 --- a/subworkflows/nf-core/fastq_align_bowtie2/main.nf +++ b/subworkflows/nf-core/fastq_align_bowtie2/main.nf @@ -21,7 +21,7 @@ workflow FASTQ_ALIGN_BOWTIE2 { // Map reads with Bowtie2 // BOWTIE2_ALIGN ( ch_reads, ch_index, save_unaligned, sort_bam ) - ch_versions = ch_versions.mix(BOWTIE2_ALIGN.out.versions.first()) + ch_versions = ch_versions.mix(BOWTIE2_ALIGN.out.versions) // // Sort, index BAM file and run samtools stats, flagstat and idxstats @@ -30,9 +30,9 @@ workflow FASTQ_ALIGN_BOWTIE2 { ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) emit: - bam_orig = BOWTIE2_ALIGN.out.aligned // channel: [ val(meta), bam ] - log_out = BOWTIE2_ALIGN.out.log // channel: [ val(meta), log ] - fastq = BOWTIE2_ALIGN.out.fastq // channel: [ val(meta), fastq ] + bam_orig = BOWTIE2_ALIGN.out.aligned // channel: [ val(meta), aligned ] + log_out = BOWTIE2_ALIGN.out.log // channel: [ val(meta), log ] + fastq = BOWTIE2_ALIGN.out.fastq // channel: [ val(meta), fastq ] bam = BAM_SORT_STATS_SAMTOOLS.out.bam // channel: [ val(meta), [ bam ] ] bai = BAM_SORT_STATS_SAMTOOLS.out.bai // channel: [ val(meta), [ bai ] ] diff --git a/subworkflows/nf-core/fastq_align_bowtie2/meta.yml b/subworkflows/nf-core/fastq_align_bowtie2/meta.yml index ad378077..58023a89 100644 --- a/subworkflows/nf-core/fastq_align_bowtie2/meta.yml +++ b/subworkflows/nf-core/fastq_align_bowtie2/meta.yml @@ -1,3 +1,4 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json name: fastq_align_bowtie2 description: Align reads to a reference genome using bowtie2 then sort with samtools keywords: @@ -5,13 +6,14 @@ keywords: - fasta - genome - reference -modules: +components: - bowtie2/align - samtools/sort - samtools/index - samtools/stats - samtools/idxstats - samtools/flagstat + - bam_sort_stats_samtools input: - meta: type: map @@ -33,9 +35,10 @@ input: Save reads that do not map to the reference (true) or discard them (false) (default: false) - sort_bam: + type: boolean description: | Save reads that do not map to the reference (true) or discard them (false) - (default: false) + default: false - ch_fasta: type: file description: Reference fasta file @@ -58,6 +61,7 @@ output: type: file description: Alignment log pattern: "*.log" -# TODO Add samtools outputs authors: - "@drpatelh" +maintainers: + - "@drpatelh" diff --git a/subworkflows/nf-core/fastq_align_bwa/main.nf b/subworkflows/nf-core/fastq_align_bwa/main.nf index 4ce4f886..9192550d 100644 --- a/subworkflows/nf-core/fastq_align_bwa/main.nf +++ b/subworkflows/nf-core/fastq_align_bwa/main.nf @@ -10,7 +10,7 @@ workflow FASTQ_ALIGN_BWA { ch_reads // channel (mandatory): [ val(meta), [ path(reads) ] ] ch_index // channel (mandatory): [ val(meta2), path(index) ] val_sort_bam // boolean (mandatory): true or false - ch_fasta // channel (optional) : [ path(fasta) ] + ch_fasta // channel (optional) : [ val(meta3), path(fasta) ] main: ch_versions = Channel.empty() diff --git a/subworkflows/nf-core/fastq_align_bwa/meta.yml b/subworkflows/nf-core/fastq_align_bwa/meta.yml index 548fec3f..fa218408 100644 --- a/subworkflows/nf-core/fastq_align_bwa/meta.yml +++ b/subworkflows/nf-core/fastq_align_bwa/meta.yml @@ -6,13 +6,15 @@ keywords: - fasta - genome - reference -modules: +components: + - bwa/mem - bwa/align - samtools/sort - samtools/index - samtools/stats - samtools/idxstats - samtools/flagstat + - bam_sort_stats_samtools input: - ch_reads: description: | @@ -22,7 +24,7 @@ input: - ch_index: description: | BWA genome index files - Structure: [ val(meta2), path(index) ] + Structure: [ val(meta), path(index) ] - val_sort_bam: type: boolean description: If true bwa modules sort resulting bam files @@ -30,9 +32,8 @@ input: - ch_fasta: type: file description: | - Optional reference fasta file. This only needs to be given if val_sort_bam = true - Structure: [ path(fasta) ] - + Optional reference fasta file. This only needs to be given if val_sort_bam = true. + Structure: [ val(meta), path(fasta) ] output: - bam_orig: description: | @@ -68,3 +69,5 @@ output: Structure: [ path(versions.yml) ] authors: - "@JoseEspinosa" +maintainers: + - "@JoseEspinosa" diff --git a/subworkflows/nf-core/fastq_align_chromap/main.nf b/subworkflows/nf-core/fastq_align_chromap/main.nf index 9d706c98..26b6a4be 100644 --- a/subworkflows/nf-core/fastq_align_chromap/main.nf +++ b/subworkflows/nf-core/fastq_align_chromap/main.nf @@ -27,7 +27,7 @@ workflow FASTQ_ALIGN_CHROMAP { // // Sort, index BAM file and run samtools stats, flagstat and idxstats // - BAM_SORT_STATS_SAMTOOLS(CHROMAP_CHROMAP.out.bam, ch_fasta.map { it[1] }) + BAM_SORT_STATS_SAMTOOLS(CHROMAP_CHROMAP.out.bam, ch_fasta) ch_versions = ch_versions.mix(BAM_SORT_STATS_SAMTOOLS.out.versions) emit: diff --git a/subworkflows/nf-core/fastq_align_chromap/meta.yml b/subworkflows/nf-core/fastq_align_chromap/meta.yml index d5f59cac..1db3eff1 100644 --- a/subworkflows/nf-core/fastq_align_chromap/meta.yml +++ b/subworkflows/nf-core/fastq_align_chromap/meta.yml @@ -10,13 +10,14 @@ keywords: - chip-seq - atac-seq - hic -modules: +components: - chromap/chromap - samtools/sort - samtools/index - samtools/stats - samtools/idxstats - samtools/flagstat + - bam_sort_stats_samtools input: - meta: type: map @@ -98,3 +99,5 @@ output: pattern: "versions.yml" authors: - "@JoseEspinosa" +maintainers: + - "@JoseEspinosa" diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml index 3b1a675c..a7df97f7 100644 --- a/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml +++ b/subworkflows/nf-core/fastq_fastqc_umitools_trimgalore/meta.yml @@ -8,7 +8,7 @@ keywords: - UMI - trimming - trimgalore -modules: +components: - fastqc - umitools/extract - trimgalore @@ -47,13 +47,14 @@ input: type: integer description: | Inputs with fewer than this reads will be filtered out of the "reads" output channel - output: - reads: type: file description: > - Extracted FASTQ files. | - For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | + Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | + + + For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. pattern: "*.{fastq.gz}" - fastqc_html: @@ -95,3 +96,6 @@ output: authors: - "@drpatelh" - "@KamilMaliszArdigen" +maintainers: + - "@drpatelh" + - "@KamilMaliszArdigen" From 26e03a5a2f102be6da132fcf019f0621eae15ecf Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 1 Feb 2024 22:14:37 +0100 Subject: [PATCH 133/201] Update modules --- modules/nf-core/bowtie2/align/environment.yml | 9 + modules/nf-core/bowtie2/align/main.nf | 17 +- modules/nf-core/bowtie2/align/meta.yml | 3 + .../bowtie2/align/tests/large_index.config | 5 + .../nf-core/bowtie2/align/tests/main.nf.test | 561 ++++++++++++++++++ .../bowtie2/align/tests/main.nf.test.snap | 263 ++++++++ .../nf-core/bowtie2/align/tests/sam.config | 5 + .../nf-core/bowtie2/align/tests/sam2.config | 5 + modules/nf-core/bowtie2/align/tests/tags.yml | 2 + modules/nf-core/bwa/mem/environment.yml | 9 + modules/nf-core/bwa/mem/main.nf | 18 +- modules/nf-core/bwa/mem/meta.yml | 3 + modules/nf-core/bwa/mem/tests/main.nf.test | 172 ++++++ .../nf-core/bwa/mem/tests/main.nf.test.snap | 126 ++++ modules/nf-core/bwa/mem/tests/tags.yml | 3 + .../nf-core/chromap/chromap/environment.yml | 8 + modules/nf-core/chromap/chromap/main.nf | 2 +- modules/nf-core/chromap/chromap/meta.yml | 5 +- modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- .../picard/markduplicates/environment.yml | 7 + modules/nf-core/picard/markduplicates/main.nf | 10 +- .../nf-core/picard/markduplicates/meta.yml | 4 + .../picard/markduplicates/tests/main.nf.test | 104 ++++ .../markduplicates/tests/main.nf.test.snap | 74 +++ .../markduplicates/tests/nextflow.config | 6 + .../picard/markduplicates/tests/tags.yml | 2 + .../nf-core/samtools/flagstat/environment.yml | 8 + modules/nf-core/samtools/flagstat/main.nf | 17 +- modules/nf-core/samtools/flagstat/meta.yml | 2 + .../samtools/flagstat/tests/main.nf.test | 36 ++ .../samtools/flagstat/tests/main.nf.test.snap | 16 + .../nf-core/samtools/flagstat/tests/tags.yml | 2 + .../nf-core/samtools/idxstats/environment.yml | 8 + modules/nf-core/samtools/idxstats/main.nf | 18 +- modules/nf-core/samtools/idxstats/meta.yml | 2 + .../samtools/idxstats/tests/main.nf.test | 36 ++ .../samtools/idxstats/tests/main.nf.test.snap | 16 + .../nf-core/samtools/idxstats/tests/tags.yml | 2 + .../nf-core/samtools/index/environment.yml | 8 + modules/nf-core/samtools/index/main.nf | 6 +- modules/nf-core/samtools/index/meta.yml | 4 + .../samtools/index/tests/csi.nextflow.config | 7 + .../nf-core/samtools/index/tests/main.nf.test | 87 +++ .../samtools/index/tests/main.nf.test.snap | 28 + modules/nf-core/samtools/index/tests/tags.yml | 2 + modules/nf-core/samtools/sort/environment.yml | 8 + modules/nf-core/samtools/sort/main.nf | 6 +- modules/nf-core/samtools/sort/meta.yml | 3 + .../nf-core/samtools/sort/tests/main.nf.test | 73 +++ .../samtools/sort/tests/main.nf.test.snap | 48 ++ .../samtools/sort/tests/nextflow.config | 7 + modules/nf-core/samtools/sort/tests/tags.yml | 3 + .../nf-core/samtools/stats/environment.yml | 8 + modules/nf-core/samtools/stats/main.nf | 6 +- modules/nf-core/samtools/stats/meta.yml | 4 + .../nf-core/samtools/stats/tests/main.nf.test | 78 +++ .../samtools/stats/tests/main.nf.test.snap | 64 ++ modules/nf-core/samtools/stats/tests/tags.yml | 2 + modules/nf-core/trimgalore/environment.yml | 7 + modules/nf-core/trimgalore/main.nf | 2 +- modules/nf-core/trimgalore/meta.yml | 4 + modules/nf-core/trimgalore/tests/main.nf.test | 105 ++++ .../trimgalore/tests/main.nf.test.snap | 148 +++++ modules/nf-core/trimgalore/tests/tags.yml | 2 + .../nf-core/umitools/extract/environment.yml | 7 + modules/nf-core/umitools/extract/main.nf | 6 +- modules/nf-core/umitools/extract/meta.yml | 17 +- .../umitools/extract/tests/main.nf.test | 35 ++ .../umitools/extract/tests/main.nf.test.snap | 10 + .../umitools/extract/tests/nextflow.config | 9 + .../nf-core/umitools/extract/tests/tags.yml | 2 + 72 files changed, 2429 insertions(+), 53 deletions(-) create mode 100644 modules/nf-core/bowtie2/align/environment.yml create mode 100644 modules/nf-core/bowtie2/align/tests/large_index.config create mode 100644 modules/nf-core/bowtie2/align/tests/main.nf.test create mode 100644 modules/nf-core/bowtie2/align/tests/main.nf.test.snap create mode 100644 modules/nf-core/bowtie2/align/tests/sam.config create mode 100644 modules/nf-core/bowtie2/align/tests/sam2.config create mode 100644 modules/nf-core/bowtie2/align/tests/tags.yml create mode 100644 modules/nf-core/bwa/mem/environment.yml create mode 100644 modules/nf-core/bwa/mem/tests/main.nf.test create mode 100644 modules/nf-core/bwa/mem/tests/main.nf.test.snap create mode 100644 modules/nf-core/bwa/mem/tests/tags.yml create mode 100644 modules/nf-core/chromap/chromap/environment.yml create mode 100644 modules/nf-core/picard/markduplicates/environment.yml create mode 100644 modules/nf-core/picard/markduplicates/tests/main.nf.test create mode 100644 modules/nf-core/picard/markduplicates/tests/main.nf.test.snap create mode 100644 modules/nf-core/picard/markduplicates/tests/nextflow.config create mode 100644 modules/nf-core/picard/markduplicates/tests/tags.yml create mode 100644 modules/nf-core/samtools/flagstat/environment.yml create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test create mode 100644 modules/nf-core/samtools/flagstat/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/flagstat/tests/tags.yml create mode 100644 modules/nf-core/samtools/idxstats/environment.yml create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test create mode 100644 modules/nf-core/samtools/idxstats/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/idxstats/tests/tags.yml create mode 100644 modules/nf-core/samtools/index/environment.yml create mode 100644 modules/nf-core/samtools/index/tests/csi.nextflow.config create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test create mode 100644 modules/nf-core/samtools/index/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/index/tests/tags.yml create mode 100644 modules/nf-core/samtools/sort/environment.yml create mode 100644 modules/nf-core/samtools/sort/tests/main.nf.test create mode 100644 modules/nf-core/samtools/sort/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/sort/tests/nextflow.config create mode 100644 modules/nf-core/samtools/sort/tests/tags.yml create mode 100644 modules/nf-core/samtools/stats/environment.yml create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test create mode 100644 modules/nf-core/samtools/stats/tests/main.nf.test.snap create mode 100644 modules/nf-core/samtools/stats/tests/tags.yml create mode 100644 modules/nf-core/trimgalore/environment.yml create mode 100644 modules/nf-core/trimgalore/tests/main.nf.test create mode 100644 modules/nf-core/trimgalore/tests/main.nf.test.snap create mode 100644 modules/nf-core/trimgalore/tests/tags.yml create mode 100644 modules/nf-core/umitools/extract/environment.yml create mode 100644 modules/nf-core/umitools/extract/tests/main.nf.test create mode 100644 modules/nf-core/umitools/extract/tests/main.nf.test.snap create mode 100644 modules/nf-core/umitools/extract/tests/nextflow.config create mode 100644 modules/nf-core/umitools/extract/tests/tags.yml diff --git a/modules/nf-core/bowtie2/align/environment.yml b/modules/nf-core/bowtie2/align/environment.yml new file mode 100644 index 00000000..d2796359 --- /dev/null +++ b/modules/nf-core/bowtie2/align/environment.yml @@ -0,0 +1,9 @@ +name: bowtie2_align +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::bowtie2=2.5.2 + - bioconda::samtools=1.18 + - conda-forge::pigz=2.6 diff --git a/modules/nf-core/bowtie2/align/main.nf b/modules/nf-core/bowtie2/align/main.nf index a77114d2..8c405ee3 100644 --- a/modules/nf-core/bowtie2/align/main.nf +++ b/modules/nf-core/bowtie2/align/main.nf @@ -2,10 +2,10 @@ process BOWTIE2_ALIGN { tag "$meta.id" label "process_high" - conda "bioconda::bowtie2=2.4.4 bioconda::samtools=1.16.1 conda-forge::pigz=2.6" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' : - 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:a0ffedb52808e102887f6ce600d092675bf3528a-0' }" + 'https://depot.galaxyproject.org/singularity/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:f70b31a2db15c023d641c32f433fb02cd04df5a6-0' : + 'biocontainers/mulled-v2-ac74a7f02cebcfcc07d8e8d1d750af9c83b4d45a:f70b31a2db15c023d641c32f433fb02cd04df5a6-0' }" input: tuple val(meta) , path(reads) @@ -52,7 +52,7 @@ process BOWTIE2_ALIGN { --threads $task.cpus \\ $unaligned \\ $args \\ - 2> ${prefix}.bowtie2.log \\ + 2> >(tee ${prefix}.bowtie2.log >&2) \\ | samtools $samtools_command $args2 --threads $task.cpus -o ${prefix}.${extension} - if [ -f ${prefix}.unmapped.fastq.1.gz ]; then @@ -76,12 +76,17 @@ process BOWTIE2_ALIGN { def prefix = task.ext.prefix ?: "${meta.id}" def extension_pattern = /(--output-fmt|-O)+\s+(\S+)/ def extension = (args2 ==~ extension_pattern) ? (args2 =~ extension_pattern)[0][2].toLowerCase() : "bam" + def create_unmapped = "" + if (meta.single_end) { + create_unmapped = save_unaligned ? "touch ${prefix}.unmapped.fastq.gz" : "" + } else { + create_unmapped = save_unaligned ? "touch ${prefix}.unmapped_1.fastq.gz && touch ${prefix}.unmapped_2.fastq.gz" : "" + } """ touch ${prefix}.${extension} touch ${prefix}.bowtie2.log - touch ${prefix}.unmapped_1.fastq.gz - touch ${prefix}.unmapped_2.fastq.gz + ${create_unmapped} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/bowtie2/align/meta.yml b/modules/nf-core/bowtie2/align/meta.yml index 60d04c12..e66811d0 100644 --- a/modules/nf-core/bowtie2/align/meta.yml +++ b/modules/nf-core/bowtie2/align/meta.yml @@ -65,3 +65,6 @@ output: authors: - "@joseespinosa" - "@drpatelh" +maintainers: + - "@joseespinosa" + - "@drpatelh" diff --git a/modules/nf-core/bowtie2/align/tests/large_index.config b/modules/nf-core/bowtie2/align/tests/large_index.config new file mode 100644 index 00000000..fdc1c59d --- /dev/null +++ b/modules/nf-core/bowtie2/align/tests/large_index.config @@ -0,0 +1,5 @@ +process { + withName: BOWTIE2_BUILD { + ext.args = '--large-index' + } +} \ No newline at end of file diff --git a/modules/nf-core/bowtie2/align/tests/main.nf.test b/modules/nf-core/bowtie2/align/tests/main.nf.test new file mode 100644 index 00000000..a478d17b --- /dev/null +++ b/modules/nf-core/bowtie2/align/tests/main.nf.test @@ -0,0 +1,561 @@ +nextflow_process { + + name "Test Process BOWTIE2_ALIGN" + script "../main.nf" + process "BOWTIE2_ALIGN" + tag "modules" + tag "modules_nfcore" + tag "bowtie2" + tag "bowtie2/align" + + test("sarscov2 - fastq, index, false, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, false, false - sam") { + + config "./sam.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).readLines()[0..4], + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, false, false - sam2") { + + config "./sam2.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).readLines()[0..4], + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, false, true - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = true //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, false, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, false, true - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = true //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, large_index, false, false - bam") { + + config "./large_index.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], large_index, false, false - bam") { + + config "./large_index.config" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, true, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = true //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, true, false - bam") { + + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = true //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + process.out.log, + process.out.fastq, + process.out.versions + ).match() } + + ) + } + + } + + test("sarscov2 - [fastq1, fastq2], index, false, false - stub") { + + options "-stub" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = false //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + file(process.out.log[0][1]).name, + process.out.fastq, + process.out.versions + ).match() } + ) + } + + } + + test("sarscov2 - fastq, index, true, false - stub") { + + options "-stub" + setup { + run("BOWTIE2_BUILD") { + script "../../build/main.nf" + process { + """ + input[0] = [ + [ id:'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + input[1] = BOWTIE2_BUILD.out.index + input[2] = true //save_unaligned + input[3] = false //sort + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.aligned[0][1]).name, + file(process.out.log[0][1]).name, + file(process.out.fastq[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/bowtie2/align/tests/main.nf.test.snap b/modules/nf-core/bowtie2/align/tests/main.nf.test.snap new file mode 100644 index 00000000..883dc7ec --- /dev/null +++ b/modules/nf-core/bowtie2/align/tests/main.nf.test.snap @@ -0,0 +1,263 @@ +{ + "sarscov2 - fastq, index, false, false - sam2": { + "content": [ + [ + "ERR5069949.2151832\t16\tMT192765.1\t17453\t42\t150M\t*\t0\t0\tACGCACATTGCTAACTAAGGGCACACTAGAACCAGAATATTTCAATTCAGTGTGTAGACTTATGAAAACTATAGGTCCAGACATGTTCCTCGGAACTTGTCGGCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTGGTTTATGA\tAAAA&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.bam + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + bwa: \$(echo \$(bwa 2>&1) | sed 's/^.*Version: //; s/Contact:.*\$//') + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/bwa/mem/meta.yml b/modules/nf-core/bwa/mem/meta.yml index 62357bf8..440fb1f9 100644 --- a/modules/nf-core/bwa/mem/meta.yml +++ b/modules/nf-core/bwa/mem/meta.yml @@ -53,3 +53,6 @@ output: authors: - "@drpatelh" - "@jeremy1805" +maintainers: + - "@drpatelh" + - "@jeremy1805" diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test b/modules/nf-core/bwa/mem/tests/main.nf.test new file mode 100644 index 00000000..b199bb70 --- /dev/null +++ b/modules/nf-core/bwa/mem/tests/main.nf.test @@ -0,0 +1,172 @@ +nextflow_process { + + name "Test Process BWA_MEM" + tag "modules_nfcore" + tag "modules" + tag "bwa" + tag "bwa/mem" + script "../main.nf" + process "BWA_MEM" + + test("Single-End") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Single-End Sort") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Paired-End") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = false + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("Paired-End Sort") { + + setup { + run("BWA_INDEX") { + script "../../index/main.nf" + process { + """ + input[0] = [ + [id: 'test'], + file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ + file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) + ] + ] + input[1] = BWA_INDEX.out.index + input[2] = true + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } +} diff --git a/modules/nf-core/bwa/mem/tests/main.nf.test.snap b/modules/nf-core/bwa/mem/tests/main.nf.test.snap new file mode 100644 index 00000000..bfb55fc7 --- /dev/null +++ b/modules/nf-core/bwa/mem/tests/main.nf.test.snap @@ -0,0 +1,126 @@ +{ + "Single-End": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,df203d7c7e8fef351408a909570c7952" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,df203d7c7e8fef351408a909570c7952" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:22.483594641" + }, + "Single-End Sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": true + }, + "test.bam:md5,8a52bd78fdcecb994c1f63897d5b431c" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:30.180783483" + }, + "Paired-End": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,9815aef9ec763a60c53c3879be2d73ae" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:38.761983007" + }, + "Paired-End Sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" + ] + ], + "1": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bam:md5,0f0cda73704c4f7ba08af482edcbbe88" + ] + ], + "versions": [ + "versions.yml:md5,a18ac8ef8cfcc7b2cc262c49d4c064f9" + ] + } + ], + "timestamp": "2023-12-04T11:01:46.284587802" + } +} \ No newline at end of file diff --git a/modules/nf-core/bwa/mem/tests/tags.yml b/modules/nf-core/bwa/mem/tests/tags.yml new file mode 100644 index 00000000..82992d1f --- /dev/null +++ b/modules/nf-core/bwa/mem/tests/tags.yml @@ -0,0 +1,3 @@ +bwa/mem: + - modules/nf-core/bwa/index/** + - modules/nf-core/bwa/mem/** diff --git a/modules/nf-core/chromap/chromap/environment.yml b/modules/nf-core/chromap/chromap/environment.yml new file mode 100644 index 00000000..a630f9c5 --- /dev/null +++ b/modules/nf-core/chromap/chromap/environment.yml @@ -0,0 +1,8 @@ +name: chromap_chromap +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::chromap=0.2.4 + - bioconda::samtools=1.16.1 diff --git a/modules/nf-core/chromap/chromap/main.nf b/modules/nf-core/chromap/chromap/main.nf index a39302e3..2cc09a43 100644 --- a/modules/nf-core/chromap/chromap/main.nf +++ b/modules/nf-core/chromap/chromap/main.nf @@ -2,7 +2,7 @@ process CHROMAP_CHROMAP { tag "$meta.id" label 'process_medium' - conda "bioconda::chromap=0.2.4 bioconda::samtools=1.16.1" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:5b2e433ab8b3d1ef098fc944b567fd98caa23f56-0' : 'biocontainers/mulled-v2-1f09f39f20b1c4ee36581dc81cc323c70e661633:5b2e433ab8b3d1ef098fc944b567fd98caa23f56-0' }" diff --git a/modules/nf-core/chromap/chromap/meta.yml b/modules/nf-core/chromap/chromap/meta.yml index 05f70cf0..140299ed 100644 --- a/modules/nf-core/chromap/chromap/meta.yml +++ b/modules/nf-core/chromap/chromap/meta.yml @@ -20,7 +20,6 @@ tools: homepage: https://github.com/haowenz/chromap documentation: https://github.com/haowenz/chromap tool_dev_url: https://github.com/haowenz/chromap - licence: ["GPL v3"] input: - meta: @@ -93,7 +92,9 @@ output: type: file description: pairs file pattern: "*.pairs.gz" - authors: - "@mahesh-panchal" - "@joseespinosa" +maintainers: + - "@mahesh-panchal" + - "@joseespinosa" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/picard/markduplicates/environment.yml b/modules/nf-core/picard/markduplicates/environment.yml new file mode 100644 index 00000000..58b795f5 --- /dev/null +++ b/modules/nf-core/picard/markduplicates/environment.yml @@ -0,0 +1,7 @@ +name: picard_markduplicates +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::picard=3.1.1 diff --git a/modules/nf-core/picard/markduplicates/main.nf b/modules/nf-core/picard/markduplicates/main.nf index facd7efb..80930cc4 100644 --- a/modules/nf-core/picard/markduplicates/main.nf +++ b/modules/nf-core/picard/markduplicates/main.nf @@ -2,10 +2,10 @@ process PICARD_MARKDUPLICATES { tag "$meta.id" label 'process_medium' - conda "bioconda::picard=3.0.0" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/picard:3.0.0--hdfd78af_1' : - 'biocontainers/picard:3.0.0--hdfd78af_1' }" + 'https://depot.galaxyproject.org/singularity/picard:3.1.1--hdfd78af_0' : + 'biocontainers/picard:3.1.1--hdfd78af_0' }" input: tuple val(meta), path(bam) @@ -30,6 +30,9 @@ process PICARD_MARKDUPLICATES { } else { avail_mem = (task.memory.mega*0.8).intValue() } + + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ picard \\ -Xmx${avail_mem}M \\ @@ -48,6 +51,7 @@ process PICARD_MARKDUPLICATES { stub: def prefix = task.ext.prefix ?: "${meta.id}" + if ("$bam" == "${prefix}.bam") error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ touch ${prefix}.bam touch ${prefix}.bam.bai diff --git a/modules/nf-core/picard/markduplicates/meta.yml b/modules/nf-core/picard/markduplicates/meta.yml index f7693d2f..1ab90c07 100644 --- a/modules/nf-core/picard/markduplicates/meta.yml +++ b/modules/nf-core/picard/markduplicates/meta.yml @@ -69,3 +69,7 @@ authors: - "@drpatelh" - "@projectoriented" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@projectoriented" + - "@ramprasadn" diff --git a/modules/nf-core/picard/markduplicates/tests/main.nf.test b/modules/nf-core/picard/markduplicates/tests/main.nf.test new file mode 100644 index 00000000..c5a29b4b --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/main.nf.test @@ -0,0 +1,104 @@ +nextflow_process { + + name "Test Process PICARD_MARKDUPLICATES" + script "../main.nf" + process "PICARD_MARKDUPLICATES" + config "./nextflow.config" + tag "modules" + tag "modules_nfcore" + tag "picard" + tag "picard/markduplicates" + + test("sarscov2 [unsorted bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("unsorted_bam_name") }, + { assert snapshot(path(process.out.metrics.get(0).get(1)).readLines()[0..2]).match("unsorted_bam_metrics") }, + { assert snapshot(process.out.versions).match("unsorted_bam_versions") } + ) + } + } + + test("sarscov2 [sorted bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta.fai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("sorted_bam_name") }, + { assert snapshot(path(process.out.metrics.get(0).get(1)).readLines()[0..2]).match("sorted_bam_metrics") }, + { assert snapshot(process.out.versions).match("sorted_bam_versions") } + ) + } + } + + test("homo_sapiens [cram]") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/cram/test.paired_end.sorted.cram', checkIfExists: true) + ]) + input[1] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + ]) + input[2] = Channel.of([ + [ id:'genome' ], + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta.fai', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(file(process.out.bam[0][1]).name).match("cram_name") }, + { assert snapshot(path(process.out.metrics.get(0).get(1)).readLines()[0..2]).match("cram_metrics") }, + { assert snapshot(process.out.versions).match("cram_versions") } + ) + } + } +} diff --git a/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap b/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap new file mode 100644 index 00000000..31c9130d --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/main.nf.test.snap @@ -0,0 +1,74 @@ +{ + "sorted_bam_versions": { + "content": [ + [ + "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" + ] + ], + "timestamp": "2024-01-19T10:26:45.092349" + }, + "unsorted_bam_name": { + "content": [ + "test.marked.bam" + ], + "timestamp": "2024-01-19T10:26:28.100755" + }, + "cram_metrics": { + "content": [ + [ + "## htsjdk.samtools.metrics.StringHeader", + "# MarkDuplicates --INPUT test.paired_end.sorted.cram --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", + "## htsjdk.samtools.metrics.StringHeader" + ] + ], + "timestamp": "2024-01-19T10:27:03.253071" + }, + "sorted_bam_metrics": { + "content": [ + [ + "## htsjdk.samtools.metrics.StringHeader", + "# MarkDuplicates --INPUT test.paired_end.sorted.bam --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", + "## htsjdk.samtools.metrics.StringHeader" + ] + ], + "timestamp": "2024-01-19T10:26:45.086503" + }, + "cram_name": { + "content": [ + "test.marked.bam" + ], + "timestamp": "2024-01-19T10:27:03.241617" + }, + "cram_versions": { + "content": [ + [ + "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" + ] + ], + "timestamp": "2024-01-19T10:27:03.26989" + }, + "unsorted_bam_versions": { + "content": [ + [ + "versions.yml:md5,b699af51b1956f3810f8a7c066e0ab17" + ] + ], + "timestamp": "2024-01-19T10:26:28.159071" + }, + "unsorted_bam_metrics": { + "content": [ + [ + "## htsjdk.samtools.metrics.StringHeader", + "# MarkDuplicates --INPUT test.paired_end.bam --OUTPUT test.marked.bam --METRICS_FILE test.marked.MarkDuplicates.metrics.txt --ASSUME_SORT_ORDER queryname --REFERENCE_SEQUENCE genome.fasta --MAX_SEQUENCES_FOR_DISK_READ_ENDS_MAP 50000 --MAX_FILE_HANDLES_FOR_READ_ENDS_MAP 8000 --SORTING_COLLECTION_SIZE_RATIO 0.25 --TAG_DUPLICATE_SET_MEMBERS false --REMOVE_SEQUENCING_DUPLICATES false --TAGGING_POLICY DontTag --CLEAR_DT true --DUPLEX_UMI false --FLOW_MODE false --FLOW_QUALITY_SUM_STRATEGY false --USE_END_IN_UNPAIRED_READS false --USE_UNPAIRED_CLIPPED_END false --UNPAIRED_END_UNCERTAINTY 0 --FLOW_SKIP_FIRST_N_FLOWS 0 --FLOW_Q_IS_KNOWN_END false --FLOW_EFFECTIVE_QUALITY_THRESHOLD 15 --ADD_PG_TAG_TO_READS true --REMOVE_DUPLICATES false --ASSUME_SORTED false --DUPLICATE_SCORING_STRATEGY SUM_OF_BASE_QUALITIES --PROGRAM_RECORD_ID MarkDuplicates --PROGRAM_GROUP_NAME MarkDuplicates --READ_NAME_REGEX --OPTICAL_DUPLICATE_PIXEL_DISTANCE 100 --MAX_OPTICAL_DUPLICATE_SET_SIZE 300000 --VERBOSITY INFO --QUIET false --VALIDATION_STRINGENCY STRICT --COMPRESSION_LEVEL 5 --MAX_RECORDS_IN_RAM 500000 --CREATE_INDEX false --CREATE_MD5_FILE false --help false --version false --showHidden false --USE_JDK_DEFLATER false --USE_JDK_INFLATER false", + "## htsjdk.samtools.metrics.StringHeader" + ] + ], + "timestamp": "2024-01-19T10:26:28.143979" + }, + "sorted_bam_name": { + "content": [ + "test.marked.bam" + ], + "timestamp": "2024-01-19T10:26:45.080116" + } +} \ No newline at end of file diff --git a/modules/nf-core/picard/markduplicates/tests/nextflow.config b/modules/nf-core/picard/markduplicates/tests/nextflow.config new file mode 100644 index 00000000..02818dd6 --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + withName: PICARD_MARKDUPLICATES { + ext.prefix = { "${meta.id}.marked" } + ext.args = '--ASSUME_SORT_ORDER queryname' + } +} diff --git a/modules/nf-core/picard/markduplicates/tests/tags.yml b/modules/nf-core/picard/markduplicates/tests/tags.yml new file mode 100644 index 00000000..4f213d62 --- /dev/null +++ b/modules/nf-core/picard/markduplicates/tests/tags.yml @@ -0,0 +1,2 @@ +picard/markduplicates: + - modules/nf-core/picard/markduplicates/** diff --git a/modules/nf-core/samtools/flagstat/environment.yml b/modules/nf-core/samtools/flagstat/environment.yml new file mode 100644 index 00000000..dd0b5c19 --- /dev/null +++ b/modules/nf-core/samtools/flagstat/environment.yml @@ -0,0 +1,8 @@ +name: samtools_flagstat +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 + - bioconda::htslib=1.18 diff --git a/modules/nf-core/samtools/flagstat/main.nf b/modules/nf-core/samtools/flagstat/main.nf index eb7e72fc..f1893d7c 100644 --- a/modules/nf-core/samtools/flagstat/main.nf +++ b/modules/nf-core/samtools/flagstat/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_FLAGSTAT { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(bam), path(bai) @@ -32,4 +32,15 @@ process SAMTOOLS_FLAGSTAT { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.flagstat + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/samtools/flagstat/meta.yml b/modules/nf-core/samtools/flagstat/meta.yml index 954225df..97991358 100644 --- a/modules/nf-core/samtools/flagstat/meta.yml +++ b/modules/nf-core/samtools/flagstat/meta.yml @@ -47,3 +47,5 @@ output: pattern: "versions.yml" authors: - "@drpatelh" +maintainers: + - "@drpatelh" diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test b/modules/nf-core/samtools/flagstat/tests/main.nf.test new file mode 100644 index 00000000..c8dd8dc9 --- /dev/null +++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process SAMTOOLS_FLAGSTAT" + script "../main.nf" + process "SAMTOOLS_FLAGSTAT" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/flagstat" + + test("BAM") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.flagstat).match() }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } +} diff --git a/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap new file mode 100644 index 00000000..880019f2 --- /dev/null +++ b/modules/nf-core/samtools/flagstat/tests/main.nf.test.snap @@ -0,0 +1,16 @@ +{ + "BAM": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.flagstat:md5,4f7ffd1e6a5e85524d443209ac97d783" + ] + ] + ], + "timestamp": "2023-11-14T15:49:22.577133" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/flagstat/tests/tags.yml b/modules/nf-core/samtools/flagstat/tests/tags.yml new file mode 100644 index 00000000..2d2b7255 --- /dev/null +++ b/modules/nf-core/samtools/flagstat/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/flagstat: + - modules/nf-core/samtools/flagstat/** diff --git a/modules/nf-core/samtools/idxstats/environment.yml b/modules/nf-core/samtools/idxstats/environment.yml new file mode 100644 index 00000000..de3ed47e --- /dev/null +++ b/modules/nf-core/samtools/idxstats/environment.yml @@ -0,0 +1,8 @@ +name: samtools_idxstats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 + - bioconda::htslib=1.18 diff --git a/modules/nf-core/samtools/idxstats/main.nf b/modules/nf-core/samtools/idxstats/main.nf index a257d700..00d916bb 100644 --- a/modules/nf-core/samtools/idxstats/main.nf +++ b/modules/nf-core/samtools/idxstats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_IDXSTATS { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(bam), path(bai) @@ -33,4 +33,16 @@ process SAMTOOLS_IDXSTATS { samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + + """ + touch ${prefix}.idxstats + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + samtools: \$(echo \$(samtools --version 2>&1) | sed 's/^.*samtools //; s/Using.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/samtools/idxstats/meta.yml b/modules/nf-core/samtools/idxstats/meta.yml index dda87e1e..344e92a3 100644 --- a/modules/nf-core/samtools/idxstats/meta.yml +++ b/modules/nf-core/samtools/idxstats/meta.yml @@ -48,3 +48,5 @@ output: pattern: "versions.yml" authors: - "@drpatelh" +maintainers: + - "@drpatelh" diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test b/modules/nf-core/samtools/idxstats/tests/main.nf.test new file mode 100644 index 00000000..f6c92150 --- /dev/null +++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test @@ -0,0 +1,36 @@ +nextflow_process { + + name "Test Process SAMTOOLS_IDXSTATS" + script "../main.nf" + process "SAMTOOLS_IDXSTATS" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/idxstats" + + test("BAM") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.idxstats).match() }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } +} diff --git a/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap new file mode 100644 index 00000000..4c6c12bd --- /dev/null +++ b/modules/nf-core/samtools/idxstats/tests/main.nf.test.snap @@ -0,0 +1,16 @@ +{ + "BAM": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + "test.idxstats:md5,df60a8c8d6621100d05178c93fb053a2" + ] + ] + ], + "timestamp": "2023-11-14T15:52:19.875194" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/idxstats/tests/tags.yml b/modules/nf-core/samtools/idxstats/tests/tags.yml new file mode 100644 index 00000000..d3057c61 --- /dev/null +++ b/modules/nf-core/samtools/idxstats/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/idxstats: + - modules/nf-core/samtools/idxstats/** diff --git a/modules/nf-core/samtools/index/environment.yml b/modules/nf-core/samtools/index/environment.yml new file mode 100644 index 00000000..81f09391 --- /dev/null +++ b/modules/nf-core/samtools/index/environment.yml @@ -0,0 +1,8 @@ +name: samtools_index +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 + - bioconda::htslib=1.18 diff --git a/modules/nf-core/samtools/index/main.nf b/modules/nf-core/samtools/index/main.nf index 0b20aa4b..8ad18fdc 100644 --- a/modules/nf-core/samtools/index/main.nf +++ b/modules/nf-core/samtools/index/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_INDEX { tag "$meta.id" label 'process_low' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(input) diff --git a/modules/nf-core/samtools/index/meta.yml b/modules/nf-core/samtools/index/meta.yml index 8bd2fa6f..01a4ee03 100644 --- a/modules/nf-core/samtools/index/meta.yml +++ b/modules/nf-core/samtools/index/meta.yml @@ -51,3 +51,7 @@ authors: - "@drpatelh" - "@ewels" - "@maxulysse" +maintainers: + - "@drpatelh" + - "@ewels" + - "@maxulysse" diff --git a/modules/nf-core/samtools/index/tests/csi.nextflow.config b/modules/nf-core/samtools/index/tests/csi.nextflow.config new file mode 100644 index 00000000..0ed260ef --- /dev/null +++ b/modules/nf-core/samtools/index/tests/csi.nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: SAMTOOLS_INDEX { + ext.args = '-c' + } + +} diff --git a/modules/nf-core/samtools/index/tests/main.nf.test b/modules/nf-core/samtools/index/tests/main.nf.test new file mode 100644 index 00000000..c76a9169 --- /dev/null +++ b/modules/nf-core/samtools/index/tests/main.nf.test @@ -0,0 +1,87 @@ +nextflow_process { + + name "Test Process SAMTOOLS_INDEX" + script "../main.nf" + process "SAMTOOLS_INDEX" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/index" + + test("sarscov2 [BAI]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.bai).match("bai") }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } + + test("homo_sapiens [CRAI]") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.crai).match("crai") }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } + + test("homo_sapiens [CSI]") { + + config "./csi.nextflow.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert path(process.out.csi.get(0).get(1)).exists() }, + { assert path(process.out.versions.get(0)).getText().contains("samtools") } + ) + } + } +} diff --git a/modules/nf-core/samtools/index/tests/main.nf.test.snap b/modules/nf-core/samtools/index/tests/main.nf.test.snap new file mode 100644 index 00000000..b3baee7f --- /dev/null +++ b/modules/nf-core/samtools/index/tests/main.nf.test.snap @@ -0,0 +1,28 @@ +{ + "crai": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.paired_end.recalibrated.sorted.cram.crai:md5,14bc3bd5c89cacc8f4541f9062429029" + ] + ] + ], + "timestamp": "2023-11-15T15:17:37.30801" + }, + "bai": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.paired_end.sorted.bam.bai:md5,704c10dd1326482448ca3073fdebc2f4" + ] + ] + ], + "timestamp": "2023-11-15T15:17:30.869234" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/index/tests/tags.yml b/modules/nf-core/samtools/index/tests/tags.yml new file mode 100644 index 00000000..e0f58a7a --- /dev/null +++ b/modules/nf-core/samtools/index/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/index: + - modules/nf-core/samtools/index/** diff --git a/modules/nf-core/samtools/sort/environment.yml b/modules/nf-core/samtools/sort/environment.yml new file mode 100644 index 00000000..f4064b72 --- /dev/null +++ b/modules/nf-core/samtools/sort/environment.yml @@ -0,0 +1,8 @@ +name: samtools_sort +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 + - bioconda::htslib=1.18 diff --git a/modules/nf-core/samtools/sort/main.nf b/modules/nf-core/samtools/sort/main.nf index 2b7753fd..4a666d42 100644 --- a/modules/nf-core/samtools/sort/main.nf +++ b/modules/nf-core/samtools/sort/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_SORT { tag "$meta.id" label 'process_medium' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(bam) diff --git a/modules/nf-core/samtools/sort/meta.yml b/modules/nf-core/samtools/sort/meta.yml index 07328431..2200de72 100644 --- a/modules/nf-core/samtools/sort/meta.yml +++ b/modules/nf-core/samtools/sort/meta.yml @@ -46,3 +46,6 @@ output: authors: - "@drpatelh" - "@ewels" +maintainers: + - "@drpatelh" + - "@ewels" diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test b/modules/nf-core/samtools/sort/tests/main.nf.test new file mode 100644 index 00000000..abb80978 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/main.nf.test @@ -0,0 +1,73 @@ +nextflow_process { + + name "Test Process SAMTOOLS_SORT" + script "../main.nf" + process "SAMTOOLS_SORT" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/sort" + + test("test_samtools_sort") { + + config "./nextflow.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + [ + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("test_samtools_sort_stub") { + + config "./nextflow.config" + options "-stub-run" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [ id:'test', single_end:false ], + [ + file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true) + ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot( + file(process.out.bam[0][1]).name, + process.out.versions + ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/samtools/sort/tests/main.nf.test.snap b/modules/nf-core/samtools/sort/tests/main.nf.test.snap new file mode 100644 index 00000000..ff722259 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/main.nf.test.snap @@ -0,0 +1,48 @@ +{ + "test_samtools_sort": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + ], + "bam": [ + [ + { + "id": "test", + "single_end": false + }, + "test.sorted.bam:md5,ea6a0fef94eb534e901f107a05a33a06" + ] + ], + "csi": [ + + ], + "versions": [ + "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + ] + } + ], + "timestamp": "2023-12-04T11:11:22.005628301" + }, + "test_samtools_sort_stub": { + "content": [ + "test.sorted.bam", + [ + "versions.yml:md5,33b6a403dc19a0d28e4219ccab0a1d80" + ] + ], + "timestamp": "2023-12-04T17:47:22.314445935" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/sort/tests/nextflow.config b/modules/nf-core/samtools/sort/tests/nextflow.config new file mode 100644 index 00000000..d0f35086 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/nextflow.config @@ -0,0 +1,7 @@ +process { + + withName: SAMTOOLS_SORT { + ext.prefix = { "${meta.id}.sorted" } + } + +} diff --git a/modules/nf-core/samtools/sort/tests/tags.yml b/modules/nf-core/samtools/sort/tests/tags.yml new file mode 100644 index 00000000..cd63ea20 --- /dev/null +++ b/modules/nf-core/samtools/sort/tests/tags.yml @@ -0,0 +1,3 @@ +samtools/sort: + - modules/nf-core/samtools/sort/** + - tests/modules/nf-core/samtools/sort/** diff --git a/modules/nf-core/samtools/stats/environment.yml b/modules/nf-core/samtools/stats/environment.yml new file mode 100644 index 00000000..b45ba90c --- /dev/null +++ b/modules/nf-core/samtools/stats/environment.yml @@ -0,0 +1,8 @@ +name: samtools_stats +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::samtools=1.18 + - bioconda::htslib=1.18 diff --git a/modules/nf-core/samtools/stats/main.nf b/modules/nf-core/samtools/stats/main.nf index 4a2607de..7539140a 100644 --- a/modules/nf-core/samtools/stats/main.nf +++ b/modules/nf-core/samtools/stats/main.nf @@ -2,10 +2,10 @@ process SAMTOOLS_STATS { tag "$meta.id" label 'process_single' - conda "bioconda::samtools=1.17" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.17--h00cdaf9_0' : - 'biocontainers/samtools:1.17--h00cdaf9_0' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : + 'biocontainers/samtools:1.18--h50ea8bc_1' }" input: tuple val(meta), path(input), path(input_index) diff --git a/modules/nf-core/samtools/stats/meta.yml b/modules/nf-core/samtools/stats/meta.yml index 90e6345f..735ff812 100644 --- a/modules/nf-core/samtools/stats/meta.yml +++ b/modules/nf-core/samtools/stats/meta.yml @@ -57,3 +57,7 @@ authors: - "@drpatelh" - "@FriederikeHanssen" - "@ramprasadn" +maintainers: + - "@drpatelh" + - "@FriederikeHanssen" + - "@ramprasadn" diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test b/modules/nf-core/samtools/stats/tests/main.nf.test new file mode 100644 index 00000000..20c3efe1 --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/main.nf.test @@ -0,0 +1,78 @@ +nextflow_process { + + name "Test Process SAMTOOLS_STATS" + script "../main.nf" + process "SAMTOOLS_STATS" + tag "modules" + tag "modules_nfcore" + tag "samtools" + tag "samtools/stats" + + test("SAMTOOLS STATS Should run without failures") { + + when { + params { + + outdir = "$outputDir" + } + process { + """ + // define inputs of the process here. + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam_bai'], checkIfExists: true) + + ] + input[1] = [[],[]] + """ + + } + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + + } + + test("SAMTOOLS CRAM Should run without failures") { + + when { + params { + + outdir = "$outputDir" + } + process { + """ + // define inputs of the process here + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram'], checkIfExists: true), + file(params.test_data['homo_sapiens']['illumina']['test_paired_end_recalibrated_sorted_cram_crai'], checkIfExists: true) + + ] + input[1] = [ + [ id:'genome' ], + file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + ] + """ + } + + + } + + then { + assertAll( + {assert process.success}, + {assert snapshot(process.out).match()} + ) + } + + } + + +} diff --git a/modules/nf-core/samtools/stats/tests/main.nf.test.snap b/modules/nf-core/samtools/stats/tests/main.nf.test.snap new file mode 100644 index 00000000..025c83a5 --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/main.nf.test.snap @@ -0,0 +1,64 @@ +{ + "SAMTOOLS STATS Should run without failures": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def" + ] + ], + "1": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,045a48208b1c6f5b8af4347fe31f4def" + ] + ], + "versions": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ] + } + ], + "timestamp": "2023-12-04T11:07:28.26821485" + }, + "SAMTOOLS CRAM Should run without failures": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43" + ] + ], + "1": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ], + "stats": [ + [ + { + "id": "test", + "single_end": false + }, + "test.stats:md5,dfbfa130d4a6925ddd1931dcd8354a43" + ] + ], + "versions": [ + "versions.yml:md5,650a365c6635001436008350ae83337c" + ] + } + ], + "timestamp": "2023-12-04T11:07:50.356233402" + } +} \ No newline at end of file diff --git a/modules/nf-core/samtools/stats/tests/tags.yml b/modules/nf-core/samtools/stats/tests/tags.yml new file mode 100644 index 00000000..7c28e30f --- /dev/null +++ b/modules/nf-core/samtools/stats/tests/tags.yml @@ -0,0 +1,2 @@ +samtools/stats: + - modules/nf-core/samtools/stats/** diff --git a/modules/nf-core/trimgalore/environment.yml b/modules/nf-core/trimgalore/environment.yml new file mode 100644 index 00000000..6cd0f51b --- /dev/null +++ b/modules/nf-core/trimgalore/environment.yml @@ -0,0 +1,7 @@ +name: trimgalore +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::trim-galore=0.6.7 diff --git a/modules/nf-core/trimgalore/main.nf b/modules/nf-core/trimgalore/main.nf index dcb77ae7..24ead871 100644 --- a/modules/nf-core/trimgalore/main.nf +++ b/modules/nf-core/trimgalore/main.nf @@ -2,7 +2,7 @@ process TRIMGALORE { tag "$meta.id" label 'process_high' - conda "bioconda::trim-galore=0.6.7" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/trim-galore:0.6.7--hdfd78af_0' : 'biocontainers/trim-galore:0.6.7--hdfd78af_0' }" diff --git a/modules/nf-core/trimgalore/meta.yml b/modules/nf-core/trimgalore/meta.yml index f84c4d77..e649088c 100644 --- a/modules/nf-core/trimgalore/meta.yml +++ b/modules/nf-core/trimgalore/meta.yml @@ -62,3 +62,7 @@ authors: - "@drpatelh" - "@ewels" - "@FelixKrueger" +maintainers: + - "@drpatelh" + - "@ewels" + - "@FelixKrueger" diff --git a/modules/nf-core/trimgalore/tests/main.nf.test b/modules/nf-core/trimgalore/tests/main.nf.test new file mode 100644 index 00000000..bc6812cc --- /dev/null +++ b/modules/nf-core/trimgalore/tests/main.nf.test @@ -0,0 +1,105 @@ +nextflow_process { + + name "Test Process TRIMGALORE" + script "../main.nf" + process "TRIMGALORE" + tag "modules" + tag "modules_nfcore" + tag "trimgalore" + + test("test_trimgalore_single_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [ id:'test', single_end:true ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + def read_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1)).linesGzip.contains(read_line) } + } + }, + { report1_lines.each { report1_line -> + { assert path(process.out.log.get(0).get(1)).getText().contains(report1_line) } + } + } + ) + } + } + + test("test_trimgalore_paired_end") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [ id:'test', single_end:false ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), + file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + def read1_lines = ["@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", + "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", + "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE + { assert path(process.out.reads.get(0).get(1).get(0)).linesGzip.contains(read1_line) } + } + }, + { read2_lines.each { read2_line -> + { assert path(process.out.reads.get(0).get(1).get(1)).linesGzip.contains(read2_line) } + } + }, + { report1_lines.each { report1_line -> + { assert path(process.out.log.get(0).get(1).get(0)).getText().contains(report1_line) } + } + }, + { report2_lines.each { report2_line -> + { assert path(process.out.log.get(0).get(1).get(1)).getText().contains(report2_line) } + } + } + ) + } + } +} diff --git a/modules/nf-core/trimgalore/tests/main.nf.test.snap b/modules/nf-core/trimgalore/tests/main.nf.test.snap new file mode 100644 index 00000000..84feacca --- /dev/null +++ b/modules/nf-core/trimgalore/tests/main.nf.test.snap @@ -0,0 +1,148 @@ +{ + "test_trimgalore_single_end": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test_trimmed.fq.gz:md5,e0a7516b8ea8d6467d6306acb2cd13c4" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastq.gz_trimming_report.txt:md5,a1ab3958205f1ddf48af623242b5b429" + ] + ], + "2": [ + + ], + "3": [ + + ], + "4": [ + + ], + "5": [ + "versions.yml:md5,47d966cbb31c80eb8f7fe860d55659b7" + ], + "html": [ + + ], + "log": [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastq.gz_trimming_report.txt:md5,a1ab3958205f1ddf48af623242b5b429" + ] + ], + "reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test_trimmed.fq.gz:md5,e0a7516b8ea8d6467d6306acb2cd13c4" + ] + ], + "unpaired": [ + + ], + "versions": [ + "versions.yml:md5,47d966cbb31c80eb8f7fe860d55659b7" + ], + "zip": [ + + ] + } + ], + "timestamp": "2023-10-17T15:24:57.782141441" + }, + "test_trimgalore_paired_end": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1_val_1.fq.gz:md5,e0a7516b8ea8d6467d6306acb2cd13c4", + "test_2_val_2.fq.gz:md5,f3d61189e6d10202da7b8686f1dbb71b" + ] + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastq.gz_trimming_report.txt:md5,315d40465412f9909bbaabf52269274d", + "test_2.fastq.gz_trimming_report.txt:md5,34436303da1c78811103427a2fb57f7b" + ] + ] + ], + "2": [ + + ], + "3": [ + + ], + "4": [ + + ], + "5": [ + "versions.yml:md5,47d966cbb31c80eb8f7fe860d55659b7" + ], + "html": [ + + ], + "log": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastq.gz_trimming_report.txt:md5,315d40465412f9909bbaabf52269274d", + "test_2.fastq.gz_trimming_report.txt:md5,34436303da1c78811103427a2fb57f7b" + ] + ] + ], + "reads": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1_val_1.fq.gz:md5,e0a7516b8ea8d6467d6306acb2cd13c4", + "test_2_val_2.fq.gz:md5,f3d61189e6d10202da7b8686f1dbb71b" + ] + ] + ], + "unpaired": [ + + ], + "versions": [ + "versions.yml:md5,47d966cbb31c80eb8f7fe860d55659b7" + ], + "zip": [ + + ] + } + ], + "timestamp": "2023-10-17T15:25:08.513589909" + } +} \ No newline at end of file diff --git a/modules/nf-core/trimgalore/tests/tags.yml b/modules/nf-core/trimgalore/tests/tags.yml new file mode 100644 index 00000000..e9937691 --- /dev/null +++ b/modules/nf-core/trimgalore/tests/tags.yml @@ -0,0 +1,2 @@ +trimgalore: + - modules/nf-core/trimgalore/** diff --git a/modules/nf-core/umitools/extract/environment.yml b/modules/nf-core/umitools/extract/environment.yml new file mode 100644 index 00000000..7d08ac0e --- /dev/null +++ b/modules/nf-core/umitools/extract/environment.yml @@ -0,0 +1,7 @@ +name: umitools_extract +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::umi_tools=1.1.4 diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf index 2f94fa93..4bd79e79 100644 --- a/modules/nf-core/umitools/extract/main.nf +++ b/modules/nf-core/umitools/extract/main.nf @@ -3,7 +3,7 @@ process UMITOOLS_EXTRACT { label "process_single" label "process_long" - conda "bioconda::umi_tools=1.1.4" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? 'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' : 'biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }" @@ -33,7 +33,7 @@ process UMITOOLS_EXTRACT { cat <<-END_VERSIONS > versions.yml "${task.process}": - umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//') + umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' ) END_VERSIONS """ } else { @@ -49,7 +49,7 @@ process UMITOOLS_EXTRACT { cat <<-END_VERSIONS > versions.yml "${task.process}": - umitools: \$(umi_tools --version 2>&1 | sed 's/^.*UMI-tools version://; s/ *\$//') + umitools: \$( umi_tools --version | sed '/version:/!d; s/.*: //' ) END_VERSIONS """ } diff --git a/modules/nf-core/umitools/extract/meta.yml b/modules/nf-core/umitools/extract/meta.yml index db64a0f8..7695b271 100644 --- a/modules/nf-core/umitools/extract/meta.yml +++ b/modules/nf-core/umitools/extract/meta.yml @@ -1,15 +1,16 @@ name: umitools_extract description: Extracts UMI barcode from a read and add it to the read name, leaving any sample barcode in place keywords: - - umitools + - UMI + - barcode - extract + - umitools tools: - umi_tools: description: > - UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) - and single cell RNA-Seq cell barcodes + UMI-tools contains tools for dealing with Unique Molecular Identifiers (UMIs)/Random Molecular Tags (RMTs) and single cell RNA-Seq cell barcodes documentation: https://umi-tools.readthedocs.io/en/latest/ - license: ["MIT"] + license: "MIT" input: - meta: type: map @@ -29,9 +30,7 @@ output: - reads: type: file description: > - Extracted FASTQ files. | - For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | - For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. + Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. pattern: "*.{fastq.gz}" - log: type: file @@ -41,7 +40,9 @@ output: type: file description: File containing software versions pattern: "versions.yml" - authors: - "@drpatelh" - "@grst" +maintainers: + - "@drpatelh" + - "@grst" diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test b/modules/nf-core/umitools/extract/tests/main.nf.test new file mode 100644 index 00000000..22242d1d --- /dev/null +++ b/modules/nf-core/umitools/extract/tests/main.nf.test @@ -0,0 +1,35 @@ +nextflow_process { + + name "Test Process UMITOOLS_EXTRACT" + script "../main.nf" + process "UMITOOLS_EXTRACT" + config "./nextflow.config" + tag "modules_nfcore" + tag "modules" + tag "umitools" + tag "umitools/extract" + + test("Should run without failures") { + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [ id:'test', single_end:true ], // meta map + [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] + ] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } +} \ No newline at end of file diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test.snap b/modules/nf-core/umitools/extract/tests/main.nf.test.snap new file mode 100644 index 00000000..6d5944f1 --- /dev/null +++ b/modules/nf-core/umitools/extract/tests/main.nf.test.snap @@ -0,0 +1,10 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,5a18da2d3a5a4de15e7aaae9082d7abb" + ] + ], + "timestamp": "2023-12-08T09:41:43.540658352" + } +} \ No newline at end of file diff --git a/modules/nf-core/umitools/extract/tests/nextflow.config b/modules/nf-core/umitools/extract/tests/nextflow.config new file mode 100644 index 00000000..c866f5a0 --- /dev/null +++ b/modules/nf-core/umitools/extract/tests/nextflow.config @@ -0,0 +1,9 @@ +process { + + publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } + + withName: UMITOOLS_EXTRACT { + ext.args = '--bc-pattern="NNNN"' + } + +} diff --git a/modules/nf-core/umitools/extract/tests/tags.yml b/modules/nf-core/umitools/extract/tests/tags.yml new file mode 100644 index 00000000..c3fb23de --- /dev/null +++ b/modules/nf-core/umitools/extract/tests/tags.yml @@ -0,0 +1,2 @@ +umitools/extract: + - modules/nf-core/umitools/extract/** From 12b6e43cdda3c3fca16aae039cddf13e8fb45014 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 1 Feb 2024 22:15:08 +0100 Subject: [PATCH 134/201] Update modules json --- modules.json | 58 ++++++++++++++++++++++++++-------------------------- 1 file changed, 29 insertions(+), 29 deletions(-) diff --git a/modules.json b/modules.json index 99214bc7..3c55d338 100644 --- a/modules.json +++ b/modules.json @@ -7,10 +7,10 @@ "nf-core": { "bowtie2/align": { "branch": "master", - "git_sha": "fe54581f8bed20e4c4a51c616c93fd3379d89820", + "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", "installed_by": [ - "modules", - "fastq_align_bowtie2" + "fastq_align_bowtie2", + "modules" ] }, "bowtie2/build": { @@ -29,18 +29,18 @@ }, "bwa/mem": { "branch": "master", - "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", + "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", "installed_by": [ - "modules", - "fastq_align_bwa" + "fastq_align_bwa", + "modules" ] }, "chromap/chromap": { "branch": "master", - "git_sha": "603ecbd9f45300c9788f197d2a15a005685b4220", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": [ - "modules", - "fastq_align_chromap" + "fastq_align_chromap", + "modules" ] }, "chromap/index": { @@ -158,10 +158,10 @@ }, "picard/markduplicates": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", "installed_by": [ - "modules", - "bam_markduplicates_picard" + "bam_markduplicates_picard", + "modules" ] }, "picard/mergesamfiles": { @@ -180,43 +180,43 @@ }, "samtools/flagstat": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", "installed_by": [ - "modules", - "bam_stats_samtools" + "bam_stats_samtools", + "modules" ] }, "samtools/idxstats": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", "installed_by": [ - "modules", - "bam_stats_samtools" + "bam_stats_samtools", + "modules" ] }, "samtools/index": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", "installed_by": [ - "modules", "bam_markduplicates_picard", - "bam_sort_stats_samtools" + "bam_sort_stats_samtools", + "modules" ] }, "samtools/sort": { "branch": "master", - "git_sha": "a0f7be95788366c1923171e358da7d049eb440f9", + "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", "installed_by": [ - "modules", - "bam_sort_stats_samtools" + "bam_sort_stats_samtools", + "modules" ] }, "samtools/stats": { "branch": "master", - "git_sha": "735e1e04e7e01751d2d6e97055bbdb6f70683cc1", + "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", "installed_by": [ - "modules", - "bam_stats_samtools" + "bam_stats_samtools", + "modules" ] }, "subread/featurecounts": { @@ -228,7 +228,7 @@ }, "trimgalore": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", "installed_by": [ "fastq_fastqc_umitools_trimgalore", "modules" @@ -243,7 +243,7 @@ }, "umitools/extract": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", + "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", "installed_by": [ "fastq_fastqc_umitools_trimgalore" ] From da58939ee5ed8e976436b36f2c35b708b4cf5f38 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 1 Feb 2024 22:15:34 +0100 Subject: [PATCH 135/201] Pass empty meta for modules needing it --- workflows/chipseq.nf | 38 +++++++++++++++++++++++++++++++++----- 1 file changed, 33 insertions(+), 5 deletions(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index b2d5831a..f3f8e517 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -173,7 +173,12 @@ workflow CHIPSEQ { FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.bwa_index, false, - PREPARE_GENOME.out.fasta + PREPARE_GENOME + .out + .fasta + .map { + [ [:], it ] + } ) ch_genome_bam = FASTQ_ALIGN_BWA.out.bam ch_genome_bam_index = FASTQ_ALIGN_BWA.out.bai @@ -193,6 +198,9 @@ workflow CHIPSEQ { params.save_unaligned, false, PREPARE_GENOME.out.fasta + .map { + [ [:], it ] + } ) ch_genome_bam = FASTQ_ALIGN_BOWTIE2.out.bam ch_genome_bam_index = FASTQ_ALIGN_BOWTIE2.out.bai @@ -209,7 +217,9 @@ workflow CHIPSEQ { FASTQ_ALIGN_CHROMAP ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.chromap_index, - PREPARE_GENOME.out.fasta + PREPARE_GENOME + .out + .fasta .map { [ [:], it ] }, @@ -233,7 +243,12 @@ workflow CHIPSEQ { ALIGN_STAR ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, PREPARE_GENOME.out.star_index, - PREPARE_GENOME.out.fasta, + PREPARE_GENOME + .out + .fasta + .map { + [ [:], it ] + }, params.seq_center ?: '' ) ch_genome_bam = ALIGN_STAR.out.bam @@ -275,8 +290,16 @@ workflow CHIPSEQ { // BAM_MARKDUPLICATES_PICARD ( PICARD_MERGESAMFILES.out.bam, - PREPARE_GENOME.out.fasta, + PREPARE_GENOME + .out + .fasta + .map { + [ [:], it ] + }, PREPARE_GENOME.out.fai + .map { + [ [:], it ] + } ) ch_versions = ch_versions.mix(BAM_MARKDUPLICATES_PICARD.out.versions) @@ -286,7 +309,12 @@ workflow CHIPSEQ { BAM_FILTER_BAMTOOLS ( BAM_MARKDUPLICATES_PICARD.out.bam.join(BAM_MARKDUPLICATES_PICARD.out.bai, by: [0]), PREPARE_GENOME.out.filtered_bed.first(), - PREPARE_GENOME.out.fasta, + PREPARE_GENOME + .out + .fasta + .map { + [ [:], it ] + }, ch_bamtools_filter_se_config, ch_bamtools_filter_pe_config ) From 1b42a379c896c47d5557b376daa83fbd0c985161 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 1 Feb 2024 22:21:41 +0100 Subject: [PATCH 136/201] Make prettier happy --- lib/WorkflowMain.groovy | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy index 70b9b3ff..56cacad8 100755 --- a/lib/WorkflowMain.groovy +++ b/lib/WorkflowMain.groovy @@ -70,7 +70,7 @@ class WorkflowMain { } return val } - + // Exit pipeline if --profile contains spaces // private static void checkProfile(profile, args, log) { From 334c21acf82048e3a91a4f0a232add59c908a6e9 Mon Sep 17 00:00:00 2001 From: Jose Espinosa-Carrasco Date: Mon, 5 Feb 2024 11:19:41 +0000 Subject: [PATCH 137/201] Fix lint --- .github/workflows/download_pipeline.yml | 2 +- .nf-core.yml | 4 +- modules.json | 185 ++++++------------------ 3 files changed, 46 insertions(+), 145 deletions(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..8a330045 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -64,4 +64,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.nf-core.yml b/.nf-core.yml index e8247adb..2e90e7d0 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -3,5 +3,5 @@ repository_type: pipeline lint: nextflow_config: - config_defaults: - - params.bamtools_filter_se_config - - params.bamtools_filter_pe_config + - params.bamtools_filter_se_config + - params.bamtools_filter_pe_config diff --git a/modules.json b/modules.json index 3c55d338..0ae8b30f 100644 --- a/modules.json +++ b/modules.json @@ -8,259 +8,177 @@ "bowtie2/align": { "branch": "master", "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", - "installed_by": [ - "fastq_align_bowtie2", - "modules" - ] + "installed_by": ["fastq_align_bowtie2", "modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", - "installed_by": [ - "fastq_align_bwa", - "modules" - ] + "installed_by": ["fastq_align_bwa", "modules"] }, "chromap/chromap": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "fastq_align_chromap", - "modules" - ] + "installed_by": ["fastq_align_chromap", "modules"] }, "chromap/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/getchromsizes": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/computematrix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "gffread": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "macs2/callpeak": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "phantompeakqualtools": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/markduplicates": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": [ - "bam_markduplicates_picard", - "modules" - ] + "installed_by": ["bam_markduplicates_picard", "modules"] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "preseq/lcextrap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/idxstats": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/index": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_markduplicates_picard", - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools", "modules"] }, "samtools/sort": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_sort_stats_samtools", "modules"] }, "samtools/stats": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "subread/featurecounts": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "trimgalore": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "umitools/extract": { "branch": "master", "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore"] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "untarfiles": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -269,57 +187,40 @@ "bam_markduplicates_picard": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "bam_sort_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "fastq_align_bowtie2", - "fastq_align_bwa", - "fastq_align_chromap" - ] + "installed_by": ["fastq_align_bowtie2", "fastq_align_bwa", "fastq_align_chromap"] }, "bam_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "bam_markduplicates_picard", - "bam_sort_stats_samtools" - ] + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_bwa": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_chromap": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } } } -} \ No newline at end of file +} From 6b1e458e1dcc96e96ea57995311eafe26d91d897 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:28:16 +0000 Subject: [PATCH 138/201] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- README.md | 5 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowChipseq.groovy | 122 ----- lib/WorkflowMain.groovy | 77 --- main.nf | 100 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 5 +- nextflow_schema.json | 1 + pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_chipseq_pipeline/main.nf | 247 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/chipseq.nf | 133 ++---- 61 files changed, 2108 insertions(+), 1406 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowChipseq.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b990088..dd9ffa53 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 1aacf8b4..1ad5c4e1 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 13edafbc..a854fd23 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index d98ab5f1..68c8deab 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index c608a466..65c68a76 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda5..0b6b1f27 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..f823210d 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e..748b4311 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd10..b706875f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f06..c3674af2 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/README.md b/README.md index bd964cef..e2d34527 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/chipseq -[![GitHub Actions CI Status](https://github.com/nf-core/chipseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/chipseq/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/chipseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/chipseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/chipseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/chipseq/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/chipseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/chipseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index f2146258..a7dc9ec4 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 20b4e844..5e7c151d 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe0..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6aba..e3ea8fa6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowChipseq.groovy b/lib/WorkflowChipseq.groovy deleted file mode 100755 index 7054c707..00000000 --- a/lib/WorkflowChipseq.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/chipseq.nf in the nf-core/chipseq pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowChipseq { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" - } - summary_section += "
    \n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 31bc8cc5..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,77 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/chipseq pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/main.nf b/main.nf index ac268f06..ef78c9e4 100644 --- a/main.nf +++ b/main.nf @@ -13,66 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +include { CHIPSEQ } from './workflows/chipseq' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_chipseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { CHIPSEQ } from './workflows/chipseq' - // -// WORKFLOW: Run main nf-core/chipseq analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_CHIPSEQ { - CHIPSEQ () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + CHIPSEQ ( + samplesheet + ) + + emit: + multiqc_report = CHIPSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_CHIPSEQ () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_CHIPSEQ ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_CHIPSEQ.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index d4a463ff..a7082c3e 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index c28658f5..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/chipseq/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fd..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d43..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
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    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630b..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a936..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24a..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b752..2212096a 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c43..354f4430 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..c204b488 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 59e48f09..f9c1e571 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index af1a8041..8d405bb0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c8..56110621 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87f..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf new file mode 100644 index 00000000..dc90ad90 --- /dev/null +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -0,0 +1,247 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +}// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..8ed4310c --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..db2030f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..f7c54bc6 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..53574ffe --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..10f948e6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..d07ce54c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..517ee54e --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 3bac800f..701d5f7d 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowChipseq.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_chipseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow CHIPSEQ { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowChipseq.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowChipseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow CHIPSEQ { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From e0e87f4a8203e81e2c763866d511d75c737892b4 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 29 Feb 2024 16:09:49 +0000 Subject: [PATCH 139/201] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +---- .github/CONTRIBUTING.md | 14 ++++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .gitpod.yml | 6 +-- README.md | 3 +- modules.json | 8 ++-- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +++--- .../utils_nfcore_chipseq_pipeline/main.nf | 10 +++-- .../tests/main.function.nf.test | 2 +- .../tests/main.function.nf.test.snap | 12 +++++- .../tests/main.workflow.nf.test | 20 ++------- .../tests/nextflow.config | 2 +- .../tests/main.function.nf.test.snap | 42 +++++++++++++++---- .../tests/main.workflow.nf.test.snap | 6 ++- .../tests/main.nf.test | 2 +- 23 files changed, 99 insertions(+), 74 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..b290e090 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 9eb44ff7..3b9272be 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/chipseq then the best place to ask is on the nf-core Slack [#chipseq](https://nfcore.slack.com/channels/chipseq) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/chipseq then the best place to ask is on the nf-core Slack [#chipseq](https://nfcore.slack.com/channels/chipseq) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 4cd6ca4f..be71c340 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/chip - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/chipseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/chipseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 1ad5c4e1..1aacf8b4 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index a854fd23..13edafbc 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 65c68a76..f1a02e20 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index f823210d..08622fd5 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b4311..073e1876 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c3674af2..d468aeaa 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.gitpod.yml b/.gitpod.yml index 363d5b1d..105a1821 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -10,13 +10,11 @@ tasks: vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/README.md b/README.md index e2d34527..939480bd 100644 --- a/README.md +++ b/README.md @@ -7,12 +7,13 @@ [![GitHub Actions CI Status](https://github.com/nf-core/chipseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/chipseq/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/chipseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/chipseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/chipseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/chipseq) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/chipseq) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23chipseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/chipseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/modules.json b/modules.json index a7082c3e..f36b13b2 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 2212096a..ca39fb67 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 354f4430..47ac352f 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b488..bfebd802 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf index dc90ad90..b5620604 100644 --- a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// Subworkflow with functionality specific to the nf-core/chipseq pipeline // /* @@ -152,7 +152,9 @@ workflow PIPELINE_COMPLETION { // def validateInputParameters() { genomeExistsError() -}// +} + +// // Validate channels from input samplesheet // def validateInputSamplesheet(input) { @@ -190,7 +192,9 @@ def genomeExistsError() { "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" error(error_string) } -}// +} + +// // Generate methods description for MultiQC // def toolCitationText() { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test index 8ed4310c..68718e4f 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -51,4 +51,4 @@ nextflow_function { ) } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap index db2030f8..e3f0baf4 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -3,10 +3,18 @@ "content": [ "v9.9.9" ], - "timestamp": "2024-01-19T11:32:36.031083" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" }, "Test Function checkCondaChannels": { "content": null, - "timestamp": "2024-01-19T11:32:50.456" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index f7c54bc6..ca964ce8 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -11,9 +11,6 @@ nextflow_workflow { test("Should run no inputs") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = false @@ -39,9 +36,6 @@ nextflow_workflow { test("Should print version") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = true @@ -68,19 +62,16 @@ nextflow_workflow { test("Should dump params") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = 'results' check_conda_channels = false input[0] = false input[1] = true - input[2] = params.outdir + input[2] = outdir input[3] = false """ } @@ -96,19 +87,16 @@ nextflow_workflow { test("Should not create params JSON if no output directory") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = null check_conda_channels = false input[0] = false input[1] = true - input[2] = null + input[2] = outdir input[3] = false """ } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 53574ffe..d0a926bf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -6,4 +6,4 @@ manifest { nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 10f948e6..1037232c 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -1,25 +1,41 @@ { "Test Function checkProfileProvided": { "content": null, - "timestamp": "2024-02-09T15:43:55.145717" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" }, "Test Function checkConfigProvided": { "content": [ true ], - "timestamp": "2024-01-19T11:34:13.548431224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" }, "Test Function nfCoreLogo": { "content": [ "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" ], - "timestamp": "2024-01-19T11:34:38.840454873" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" }, "Test Function workflowCitation": { "content": [ "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" ], - "timestamp": "2024-01-19T11:34:22.24352016" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" }, "Test Function without logColours": { "content": [ @@ -73,13 +89,21 @@ "biwhite": "" } ], - "timestamp": "2024-01-19T11:35:04.418416984" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" }, "Test Function dashedLine": { "content": [ "-\u001b[2m----------------------------------------------------\u001b[0m-" ], - "timestamp": "2024-01-19T11:34:55.420000755" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" }, "Test Function with logColours": { "content": [ @@ -133,6 +157,10 @@ "biwhite": "\u001b[1;97m" } ], - "timestamp": "2024-01-19T11:35:13.436366565" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap index d07ce54c..859d1030 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -10,6 +10,10 @@ ] } ], - "timestamp": "2024-01-19T11:35:22.538940073" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test index 517ee54e..5784a33f 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -197,4 +197,4 @@ nextflow_workflow { ) } } -} \ No newline at end of file +} From a81ee331e528280294f581d594c5c80bdb48d53e Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Wed, 8 May 2024 13:58:04 +0000 Subject: [PATCH 140/201] Template update for nf-core/tools version 2.14.0 --- .editorconfig | 6 +- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 10 +- .github/workflows/awstest.yml | 12 +- .github/workflows/ci.yml | 4 +- .github/workflows/download_pipeline.yml | 22 ++- .github/workflows/fix-linting.yml | 6 +- .github/workflows/linting.yml | 18 +- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 6 +- .nf-core.yml | 1 + .pre-commit-config.yaml | 3 + README.md | 2 +- conf/base.config | 3 - conf/modules.config | 8 - conf/test.config | 2 +- conf/test_full.config | 2 +- docs/usage.md | 2 + modules.json | 4 +- modules/nf-core/fastqc/main.nf | 6 + nextflow.config | 176 +++++++++--------- nextflow_schema.json | 7 + pyproject.toml | 15 -- .../utils_nfcore_chipseq_pipeline/main.nf | 16 +- .../nf-core/utils_nfcore_pipeline/main.nf | 8 +- workflows/chipseq.nf | 46 +++-- 26 files changed, 220 insertions(+), 169 deletions(-) delete mode 100644 pyproject.toml diff --git a/.editorconfig b/.editorconfig index dd9ffa53..72dda289 100644 --- a/.editorconfig +++ b/.editorconfig @@ -28,10 +28,6 @@ indent_style = unset [/assets/email*] indent_size = unset -# ignore Readme -[README.md] -indent_style = unset - -# ignore python +# ignore python and markdown [*.{py,md}] indent_style = unset diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index be71c340..4cd6ca4f 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/chip - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/chipseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/chipseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). +- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 1aacf8b4..077f31a6 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -8,12 +8,12 @@ on: types: [published] workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS full tests if: github.repository == 'nf-core/chipseq' runs-on: ubuntu-latest steps: - - name: Launch workflow via tower + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) @@ -33,7 +33,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 13edafbc..bf518006 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -5,13 +5,13 @@ name: nf-core AWS test on: workflow_dispatch: jobs: - run-tower: + run-platform: name: Run AWS tests if: github.repository == 'nf-core/chipseq' runs-on: ubuntu-latest steps: - # Launch workflow using Tower CLI tool action - - name: Launch workflow via tower + # Launch workflow using Seqera Platform CLI tool action + - name: Launch workflow via Seqera Platform uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} @@ -27,7 +27,7 @@ jobs: - uses: actions/upload-artifact@v4 with: - name: Tower debug log file + name: Seqera Platform debug log file path: | - tower_action_*.log - tower_action_*.json + seqera_platform_action_*.log + seqera_platform_action_*.json diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index f1a02e20..ec824da3 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,10 +28,10 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 08622fd5..2d20d644 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -14,6 +14,8 @@ on: pull_request: types: - opened + - edited + - synchronize branches: - master pull_request_target: @@ -28,11 +30,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: @@ -65,8 +70,17 @@ jobs: - name: Inspect download run: tree ./${{ env.REPOTITLE_LOWERCASE }} - - name: Run the downloaded pipeline + - name: Run the downloaded pipeline (stub) + id: stub_run_pipeline + continue-on-error: true env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + - name: Run the downloaded pipeline (stub run not supported) + id: run_pipeline + if: ${{ job.steps.stub_run_pipeline.status == failure() }} + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index d7e08c32..7b84c0be 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -13,7 +13,7 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} @@ -32,9 +32,9 @@ jobs: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} # Install and run pre-commit - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" - name: Install pre-commit run: pip install pre-commit diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 073e1876..a3fb2541 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,12 +14,12 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + - uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - - name: Set up Python 3.11 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - name: Set up Python 3.12 + uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: 3.11 + python-version: "3.12" cache: "pip" - name: Install pre-commit @@ -32,14 +32,14 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 + uses: actions/checkout@0ad4b8fadaa221de15dcec353f45205ec38ea70b # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@v2 - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: - python-version: "3.11" + python-version: "3.12" architecture: "x64" - name: Install dependencies @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + uses: actions/upload-artifact@65462800fd760344b1a7b4382951275a0abb4808 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index b706875f..40acc23f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 + uses: dawidd6/action-download-artifact@09f2f74827fd3a8607589e5ad7f9398816f540fe # v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index d468aeaa..03ecfcf7 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT + echo "topics=$(curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ')" >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: @@ -25,13 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} - ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + ${{ steps.get_topics.outputs.topics }} #nfcore #openscience #nextflow #bioinformatics send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + - uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.10" - name: Install dependencies diff --git a/.nf-core.yml b/.nf-core.yml index 3805dc81..d6daa403 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1 +1,2 @@ repository_type: pipeline +nf_core_version: "2.14.0" diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index af57081f..4dc0f1dc 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -3,6 +3,9 @@ repos: rev: "v3.1.0" hooks: - id: prettier + additional_dependencies: + - prettier@3.2.5 + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python rev: "2.7.3" hooks: diff --git a/README.md b/README.md index 939480bd..b4d05fd0 100644 --- a/README.md +++ b/README.md @@ -13,7 +13,7 @@ [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/chipseq) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://cloud.seqera.io/launch?pipeline=https://github.com/nf-core/chipseq) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23chipseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/chipseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/conf/base.config b/conf/base.config index 6ff52bc0..dfa8a0da 100644 --- a/conf/base.config +++ b/conf/base.config @@ -59,7 +59,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/conf/modules.config b/conf/modules.config index e3ea8fa6..d203d2b6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -22,14 +22,6 @@ process { ext.args = '--quiet' } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'MULTIQC' { ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' } publishDir = [ diff --git a/conf/test.config b/conf/test.config index 2bf8a1fa..0ca49358 100644 --- a/conf/test.config +++ b/conf/test.config @@ -22,7 +22,7 @@ params { // Input data // TODO nf-core: Specify the paths to your test data on nf-core/test-datasets // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv' // Genome references genome = 'R64-1-1' diff --git a/conf/test_full.config b/conf/test_full.config index 5c3bc080..b9d9918e 100644 --- a/conf/test_full.config +++ b/conf/test_full.config @@ -17,7 +17,7 @@ params { // Input data for full size test // TODO nf-core: Specify the paths to your full test data ( on nf-core/test-datasets or directly in repositories, e.g. SRA) // TODO nf-core: Give any required params for the test so that command line flags are not needed - input = 'https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' + input = params.pipelines_testdata_base_path + 'viralrecon/samplesheet/samplesheet_full_illumina_amplicon.csv' // Genome references genome = 'R64-1-1' diff --git a/docs/usage.md b/docs/usage.md index 09080185..580dc4a2 100644 --- a/docs/usage.md +++ b/docs/usage.md @@ -156,6 +156,8 @@ If `-profile` is not specified, the pipeline will run locally and expect all sof - A generic configuration profile to be used with [Charliecloud](https://hpc.github.io/charliecloud/) - `apptainer` - A generic configuration profile to be used with [Apptainer](https://apptainer.org/) +- `wave` + - A generic configuration profile to enable [Wave](https://seqera.io/wave/) containers. Use together with one of the above (requires Nextflow ` 24.03.0-edge` or later). - `conda` - A generic configuration profile to be used with [Conda](https://conda.io/docs/). Please only use Conda as a last resort i.e. when it's not possible to run the pipeline with Docker, Singularity, Podman, Shifter, Charliecloud, or Apptainer. diff --git a/modules.json b/modules.json index f36b13b2..afe95daf 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["modules"] }, "multiqc": { @@ -26,7 +26,7 @@ }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9e19a74c..d79f1c86 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -25,6 +25,11 @@ process FASTQC { def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // FastQC memory value allowed range (100 - 10000) + def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) + """ printf "%s %s\\n" $rename_to | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name @@ -33,6 +38,7 @@ process FASTQC { fastqc \\ $args \\ --threads $task.cpus \\ + --memory $fastqc_memory \\ $renamed_files cat <<-END_VERSIONS > versions.yml diff --git a/nextflow.config b/nextflow.config index f9c1e571..563221d5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,7 +16,8 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - fasta = null// MultiQC options + + // MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -24,15 +25,16 @@ params { multiqc_methods_description = null // Boilerplate options - outdir = null - publish_dir_mode = 'copy' - email = null - email_on_fail = null - plaintext_email = false - monochrome_logs = false - hook_url = null - help = false - version = false + outdir = null + publish_dir_mode = 'copy' + email = null + email_on_fail = null + plaintext_email = false + monochrome_logs = false + hook_url = null + help = false + version = false + pipelines_testdata_base_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/' // Config options config_profile_name = null @@ -68,103 +70,109 @@ try { } // Load nf-core/chipseq custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! -// try { -// includeConfig "${params.custom_config_base}/pipeline/chipseq.config" -// } catch (Exception e) { -// System.err.println("WARNING: Could not load nf-core/config/chipseq profiles: ${params.custom_config_base}/pipeline/chipseq.config") -// } +try { + includeConfig "${params.custom_config_base}/pipeline/chipseq.config" +} catch (Exception e) { + System.err.println("WARNING: Could not load nf-core/config/chipseq profiles: ${params.custom_config_base}/pipeline/chipseq.config") +} profiles { debug { - dumpHashes = true - process.beforeScript = 'echo $HOSTNAME' - cleanup = false + dumpHashes = true + process.beforeScript = 'echo $HOSTNAME' + cleanup = false nextflow.enable.configProcessNamesValidation = true } conda { - conda.enabled = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - channels = ['conda-forge', 'bioconda', 'defaults'] - apptainer.enabled = false + conda.enabled = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + conda.channels = ['conda-forge', 'bioconda', 'defaults'] + apptainer.enabled = false } mamba { - conda.enabled = true - conda.useMamba = true - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + conda.enabled = true + conda.useMamba = true + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } docker { - docker.enabled = true - conda.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false - docker.runOptions = '-u $(id -u):$(id -g)' + docker.enabled = true + conda.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false + docker.runOptions = '-u $(id -u):$(id -g)' } arm { - docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' + docker.runOptions = '-u $(id -u):$(id -g) --platform=linux/amd64' } singularity { - singularity.enabled = true - singularity.autoMounts = true - conda.enabled = false - docker.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + singularity.enabled = true + singularity.autoMounts = true + conda.enabled = false + docker.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } podman { - podman.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - shifter.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + podman.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + shifter.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } shifter { - shifter.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - charliecloud.enabled = false - apptainer.enabled = false + shifter.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + charliecloud.enabled = false + apptainer.enabled = false } charliecloud { - charliecloud.enabled = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - apptainer.enabled = false + charliecloud.enabled = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + apptainer.enabled = false } apptainer { - apptainer.enabled = true - apptainer.autoMounts = true - conda.enabled = false - docker.enabled = false - singularity.enabled = false - podman.enabled = false - shifter.enabled = false - charliecloud.enabled = false + apptainer.enabled = true + apptainer.autoMounts = true + conda.enabled = false + docker.enabled = false + singularity.enabled = false + podman.enabled = false + shifter.enabled = false + charliecloud.enabled = false + } + wave { + apptainer.ociAutoPull = true + singularity.ociAutoPull = true + wave.enabled = true + wave.freeze = true + wave.strategy = 'conda,container' } gitpod { - executor.name = 'local' - executor.cpus = 4 - executor.memory = 8.GB + executor.name = 'local' + executor.cpus = 4 + executor.memory = 8.GB } test { includeConfig 'conf/test.config' } test_full { includeConfig 'conf/test_full.config' } diff --git a/nextflow_schema.json b/nextflow_schema.json index 8d405bb0..9f702a2f 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -265,6 +265,13 @@ "description": "Validation of parameters in lenient more.", "hidden": true, "help_text": "Allows string values that are parseable as numbers or booleans. For further information see [JSONSchema docs](https://github.com/everit-org/json-schema#lenient-mode)." + }, + "pipelines_testdata_base_path": { + "type": "string", + "fa_icon": "far fa-check-circle", + "description": "Base URL or local path to location of pipeline test dataset files", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/", + "hidden": true } } } diff --git a/pyproject.toml b/pyproject.toml deleted file mode 100644 index 56110621..00000000 --- a/pyproject.toml +++ /dev/null @@ -1,15 +0,0 @@ -# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. -# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.ruff] -line-length = 120 -target-version = "py38" -cache-dir = "~/.cache/ruff" - -[tool.ruff.lint] -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] - -[tool.ruff.lint.isort] -known-first-party = ["nf_core"] - -[tool.ruff.lint.per-file-ignores] -"__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf index b5620604..ea75b693 100644 --- a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -140,6 +140,10 @@ workflow PIPELINE_COMPLETION { imNotification(summary_params, hook_url) } } + + workflow.onError { + log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting" + } } /* @@ -230,8 +234,16 @@ def methodsDescriptionText(mqc_methods_yaml) { meta["manifest_map"] = workflow.manifest.toMap() // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + if (meta.manifest_map.doi) { + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + def temp_doi_ref = "" + String[] manifest_doi = meta.manifest_map.doi.tokenize(",") + for (String doi_ref: manifest_doi) temp_doi_ref += "(doi: ${doi_ref.replace("https://doi.org/", "").replace(" ", "")}), " + meta["doi_text"] = temp_doi_ref.substring(0, temp_doi_ref.length() - 2) + } else meta["doi_text"] = "" + meta["nodoi_text"] = meta.manifest_map.doi ? "" : "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " // Tool references meta["tool_citations"] = "" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf index a8b55d6f..14558c39 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -65,9 +65,15 @@ def checkProfileProvided(nextflow_cli_args) { // Citation string for pipeline // def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + "* The pipeline\n" + - " ${workflow.manifest.doi}\n\n" + + temp_doi_ref + "\n" + "* The nf-core framework\n" + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + "* Software dependencies\n" + diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 701d5f7d..277a9dc0 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -40,22 +40,44 @@ workflow CHIPSEQ { // Collate and save software versions // softwareVersionsToYAML(ch_versions) - .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) - .set { ch_collated_versions } + .collectFile( + storeDir: "${params.outdir}/pipeline_info", + name: 'nf_core_pipeline_software_mqc_versions.yml', + sort: true, + newLine: true + ).set { ch_collated_versions } // // MODULE: MultiQC // - ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) - ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() - ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() - summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") - ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) - ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) + ch_multiqc_config = Channel.fromPath( + "$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? + Channel.fromPath(params.multiqc_config, checkIfExists: true) : + Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? + Channel.fromPath(params.multiqc_logo, checkIfExists: true) : + Channel.empty() + + summary_params = paramsSummaryMap( + workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? + file(params.multiqc_methods_description, checkIfExists: true) : + file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value( + methodsDescriptionText(ch_multiqc_custom_methods_description)) + + ch_multiqc_files = ch_multiqc_files.mix( + ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix( + ch_methods_description.collectFile( + name: 'methods_description_mqc.yaml', + sort: true + ) + ) MULTIQC ( ch_multiqc_files.collect(), From d4b1a539a92d0bb6a6156455e7a80a4a668559d1 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 9 May 2024 11:39:05 +0000 Subject: [PATCH 141/201] Template update for nf-core/tools version 2.14.1 --- .github/workflows/linting.yml | 1 - .nf-core.yml | 2 +- 2 files changed, 1 insertion(+), 2 deletions(-) diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index a3fb2541..1fcafe88 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -20,7 +20,6 @@ jobs: uses: actions/setup-python@82c7e631bb3cdc910f68e0081d67478d79c6982d # v5 with: python-version: "3.12" - cache: "pip" - name: Install pre-commit run: pip install pre-commit diff --git a/.nf-core.yml b/.nf-core.yml index d6daa403..e0b85a77 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -1,2 +1,2 @@ repository_type: pipeline -nf_core_version: "2.14.0" +nf_core_version: "2.14.1" From 5712425cce51bd9923ac4e3c373f36e9a18435e4 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:47:15 +0200 Subject: [PATCH 142/201] Get rid of lib folder --- lib/NfcoreTemplate.groovy | 356 ------------------------------------- lib/Utils.groovy | 47 ----- lib/WorkflowChipseq.groovy | 156 ---------------- lib/WorkflowMain.groovy | 84 --------- 4 files changed, 643 deletions(-) delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowChipseq.groovy delete mode 100755 lib/WorkflowMain.groovy diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowChipseq.groovy b/lib/WorkflowChipseq.groovy deleted file mode 100755 index 1dbd7ffc..00000000 --- a/lib/WorkflowChipseq.groovy +++ /dev/null @@ -1,156 +0,0 @@ -// -// This file holds several functions specific to the workflow/chipseq.nf in the nf-core/chipseq pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowChipseq { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - genomeExistsError(params, log) - - if (!params.gtf && !params.gff) { - def error_string = "No GTF or GFF3 annotation specified! The pipeline requires at least one of these files." - Nextflow.error(error_string) - } - - if (params.gtf && params.gff) { - gtfGffWarn(log) - } - - if (!params.macs_gsize) { - macsGsizeWarn(log) - } - - if (!params.read_length && !params.macs_gsize) { - def error_string = "Both '--read_length' and '--macs_gsize' not specified! Please specify either to infer MACS2 genome size for peak calling." - Nextflow.error(error_string) - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

    $group

    \n" - summary_section += "
    \n" - for (param in group_params.keySet()) { - summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" - } - summary_section += "
    \n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } - - // - // Print a warning if both GTF and GFF have been provided - // - private static void gtfGffWarn(log) { - log.warn "=============================================================================\n" + - " Both '--gtf' and '--gff' parameters have been provided.\n" + - " Using GTF file as priority.\n" + - "===================================================================================" - } - - // - // Print a warning if macs_gsize parameter has not been provided - // - private static void macsGsizeWarn(log) { - log.warn "=============================================================================\n" + - " --macs_gsize parameter has not been provided.\n" + - " It will be auto-calculated by 'khmer unique-kmers.py' using the '--read_length' parameter.\n" + - " Explicitly provide '--macs_gsize' to change this behaviour.\n" + - "===================================================================================" - } - -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index 56cacad8..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,84 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/chipseq pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - "* The pipeline\n" + - " https://doi.org/10.5281/zenodo.3240506\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Get macs genome size (macs_gsize) - // - public static Long getMacsGsize(params) { - def val = null - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey('macs_gsize')) { - if (params.genomes[ params.genome ][ 'macs_gsize' ].containsKey(params.read_length.toString())) { - val = params.genomes[ params.genome ][ 'macs_gsize' ][ params.read_length.toString() ] - } - } - } - return val - } - - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} From 198d114f77d1f1867e553b87a86177f96cfeb9d5 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:48:47 +0200 Subject: [PATCH 143/201] Get rid of deprecated modules --- .../dumpsoftwareversions/environment.yml | 7 -- .../custom/dumpsoftwareversions/main.nf | 24 ----- .../custom/dumpsoftwareversions/meta.yml | 37 ------- .../templates/dumpsoftwareversions.py | 101 ------------------ .../dumpsoftwareversions/tests/main.nf.test | 43 -------- .../tests/main.nf.test.snap | 33 ------ .../dumpsoftwareversions/tests/tags.yml | 2 - 7 files changed, 247 deletions(-) delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fd..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index da033408..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,101 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import yaml -import platform -from textwrap import dedent - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    File typeConventional base calls
    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630b..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a936..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24a..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** From 39e10831960909f2f77caac367dd15189feaa33c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:50:13 +0200 Subject: [PATCH 144/201] Add pipeline functions subworkflow --- .../utils_nfcore_chipseq_pipeline/main.nf | 268 ++++++++++++++++++ 1 file changed, 268 insertions(+) create mode 100644 subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf new file mode 100644 index 00000000..92daace0 --- /dev/null +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -0,0 +1,268 @@ +// +// Subworkflow with functionality specific to the nf-core/chipseq pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + + main: + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --genome GRCh37 --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + + // + // Custom validation for pipeline parameters + // + validateInputParameters() + +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } + + workflow.onError { + log.error "Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting" + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + + genomeExistsError() + + if (!params.fasta) { + error("Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file.") + } + + if (!params.gtf && !params.gff) { + error("No GTF or GFF3 annotation specified! The pipeline requires at least one of these files.") + } + + if (params.gtf && params.gff) { + gtfGffWarn(log) + } + + if (!params.macs_gsize) { + macsGsizeWarn(log) + } + + if (!params.read_length && !params.macs_gsize) { + error ("Both '--read_length' and '--macs_gsize' not specified! Please specify either to infer MACS2 genome size for peak calling.") + } +} + +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +} + +// +// Get macs genome size (macs_gsize) +// +def getMacsGsize(params) { + def val = null + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey('macs_gsize')) { + if (params.genomes[ params.genome ][ 'macs_gsize' ].containsKey(params.read_length.toString())) { + val = params.genomes[ params.genome ][ 'macs_gsize' ][ params.read_length.toString() ] + } + } + } + return val +} + +// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} + +// +// Print a warning if both GTF and GFF have been provided +// +def gtfGffWarn(log) { + log.warn "=============================================================================\n" + + " Both '--gtf' and '--gff' parameters have been provided.\n" + + " Using GTF file as priority.\n" + + "===================================================================================" +} + +// +// Print a warning if macs_gsize parameter has not been provided +// +def macsGsizeWarn(log) { + log.warn "=============================================================================\n" + + " --macs_gsize parameter has not been provided.\n" + + " It will be auto-calculated by 'khmer unique-kmers.py' using the '--read_length' parameter.\n" + + " Explicitly provide '--macs_gsize macs2_genome_size' to change this behaviour.\n" + + "===================================================================================" +} \ No newline at end of file From bda31b26bc469e012622d241f2d35886bf94574f Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:51:36 +0200 Subject: [PATCH 145/201] Install utils subworkflows --- modules.json | 207 ++++++-- .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 20 + .../tests/main.workflow.nf.test | 111 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 446 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 166 +++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 19 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + 21 files changed, 1753 insertions(+), 47 deletions(-) create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/modules.json b/modules.json index 0ae8b30f..285d38b9 100644 --- a/modules.json +++ b/modules.json @@ -8,177 +8,252 @@ "bowtie2/align": { "branch": "master", "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", - "installed_by": ["fastq_align_bowtie2", "modules"] + "installed_by": [ + "fastq_align_bowtie2", + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", - "installed_by": ["fastq_align_bwa", "modules"] + "installed_by": [ + "fastq_align_bwa", + "modules" + ] }, "chromap/chromap": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["fastq_align_chromap", "modules"] + "installed_by": [ + "fastq_align_chromap", + "modules" + ] }, "chromap/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] - }, - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/getchromsizes": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/computematrix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "gffread": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "macs2/callpeak": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "phantompeakqualtools": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/markduplicates": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": ["bam_markduplicates_picard", "modules"] + "installed_by": [ + "bam_markduplicates_picard", + "modules" + ] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "preseq/lcextrap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/flagstat": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_stats_samtools", "modules"] + "installed_by": [ + "bam_stats_samtools", + "modules" + ] }, "samtools/idxstats": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_stats_samtools", "modules"] + "installed_by": [ + "bam_stats_samtools", + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools", "modules"] + "installed_by": [ + "bam_markduplicates_picard", + "bam_sort_stats_samtools", + "modules" + ] }, "samtools/sort": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_sort_stats_samtools", "modules"] + "installed_by": [ + "bam_sort_stats_samtools", + "modules" + ] }, "samtools/stats": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": ["bam_stats_samtools", "modules"] + "installed_by": [ + "bam_stats_samtools", + "modules" + ] }, "subread/featurecounts": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "trimgalore": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "umitools/extract": { "branch": "master", "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", - "installed_by": ["fastq_fastqc_umitools_trimgalore"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore" + ] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "untarfiles": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -187,40 +262,78 @@ "bam_markduplicates_picard": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "bam_sort_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["fastq_align_bowtie2", "fastq_align_bwa", "fastq_align_chromap"] + "installed_by": [ + "fastq_align_bowtie2", + "fastq_align_bwa", + "fastq_align_chromap" + ] }, "bam_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] + "installed_by": [ + "bam_markduplicates_picard", + "bam_sort_stats_samtools" + ] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "fastq_align_bwa": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "fastq_align_chromap": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] + }, + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": [ + "subworkflows" + ] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", + "installed_by": [ + "subworkflows" + ] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", + "installed_by": [ + "subworkflows" + ] } } } } } -} +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..68718e4f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..e3f0baf4 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,20 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" + }, + "Test Function checkCondaChannels": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..ca964ce8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,111 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = 'results' + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + workflow { + """ + print_version = false + dump_parameters = true + outdir = null + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..14558c39 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,446 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + def temp_doi_ref = "" + String[] manifest_doi = workflow.manifest.doi.tokenize(",") + // Using a loop to handle multiple DOIs + // Removing `https://doi.org/` to handle pipelines using DOIs vs DOI resolvers + // Removing ` ` since the manifest.doi is a string and not a proper list + for (String doi_ref: manifest_doi) temp_doi_ref += " https://doi.org/${doi_ref.replace('https://doi.org/', '').replace(' ', '')}\n" + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + temp_doi_ref + "\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..1037232c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,166 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..859d1030 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,19 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..5784a33f --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** From 1b3dd1b39dcfb4e7677f0a4e9ba8318c016614c9 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:52:20 +0200 Subject: [PATCH 146/201] Update modules config --- conf/modules.config | 8 -------- 1 file changed, 8 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 7f923394..59af9e66 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -30,14 +30,6 @@ process { ] } - withName: CUSTOM_DUMPSOFTWAREVERSIONS { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - pattern: '*_versions.yml' - ] - } - withName: 'KHMER_UNIQUEKMERS' { publishDir = [ enabled: false ] } From 4cf206c1f55ff2d35182d9da982dc27bdacc0864 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:52:55 +0200 Subject: [PATCH 147/201] Update multiqc --- modules/local/multiqc.nf | 4 +--- 1 file changed, 1 insertion(+), 3 deletions(-) diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index f5b6092e..31da0b07 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -7,11 +7,9 @@ process MULTIQC { 'biocontainers/multiqc:1.13a--pyhdfd78af_1' }" input: + path workflow_summary path multiqc_config path mqc_custom_config - path software_versions - path workflow_summary - path methods_description path logo path ('fastqc/*') From 933dede70004a452d09e5bf3e1f8b56e6a9b2fd0 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:53:09 +0200 Subject: [PATCH 148/201] Update prepare_genome --- subworkflows/local/prepare_genome.nf | 95 +++++++++++++++------------- 1 file changed, 52 insertions(+), 43 deletions(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index bd7033cb..f3bc02eb 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -27,39 +27,50 @@ include { STAR_GENOMEGENERATE } from '../../modules/local/star_genomegenera workflow PREPARE_GENOME { take: + genome // string: genome name + genomes // map: genome attributes prepare_tool_index // string : tool to prepare index for - + fasta // path: path to genome fasta file + gtf // file: /path/to/genome.gtf + gff // file: /path/to/genome.gff + blacklist // file: /path/to/blacklist.bed + gene_bed // file: /path/to/gene.bed + bwa_index // file: /path/to/bwa/index/ + bowtie2_index // file: /path/to/bowtie2/index/ + chromap_index // file: /path/to/chromap/index/ + star_index // file: /path/to/star/index/ + main: ch_versions = Channel.empty() - + // // Uncompress genome fasta file if required // ch_fasta = Channel.empty() - if (params.fasta.endsWith('.gz')) { - ch_fasta = GUNZIP_FASTA ( [ [:], params.fasta ] ).gunzip.map{ it[1] } + if (fasta.endsWith('.gz')) { + ch_fasta = GUNZIP_FASTA ( [ [:], fasta ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_FASTA.out.versions) } else { - ch_fasta = Channel.value(file(params.fasta)) + ch_fasta = Channel.value(file(fasta)) } // // Uncompress GTF annotation file or create from GFF3 if required // - if (params.gtf) { - if (params.gtf.endsWith('.gz')) { - ch_gtf = GUNZIP_GTF ( [ [:], params.gtf ] ).gunzip.map{ it[1] } + if (gtf) { + if (gtf.endsWith('.gz')) { + ch_gtf = GUNZIP_GTF ( [ [:], gtf ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_GTF.out.versions) } else { - ch_gtf = file(params.gtf) + ch_gtf = Channel.value(file(gtf)) } - } else if (params.gff) { - if (params.gff.endsWith('.gz')) { - ch_gff = GUNZIP_GFF ( [ [:], params.gff ] ).gunzip.map{ it[1] } + } else if (gff) { + if (gff.endsWith('.gz')) { + ch_gff = GUNZIP_GFF ( [ [:], gff ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_GFF.out.versions) } else { - ch_gff = file(params.gff) + ch_gff = Channel.value(file(gff)) } ch_gtf = GFFREAD ( ch_gff ).gtf ch_versions = ch_versions.mix(GFFREAD.out.versions) @@ -69,12 +80,12 @@ workflow PREPARE_GENOME { // Uncompress blacklist file if required // ch_blacklist = Channel.empty() - if (params.blacklist) { - if (params.blacklist.endsWith('.gz')) { - ch_blacklist = GUNZIP_BLACKLIST ( [ [:], params.blacklist ] ).gunzip.map{ it[1] } + if (blacklist) { + if (blacklist.endsWith('.gz')) { + ch_blacklist = GUNZIP_BLACKLIST ( [ [:], blacklist ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_BLACKLIST.out.versions) } else { - ch_blacklist = Channel.fromPath(file(params.blacklist)) + ch_blacklist = Channel.value(file(blacklist)) } } @@ -85,10 +96,10 @@ workflow PREPARE_GENOME { // If --gtf is supplied along with --genome // Make gene bed from supplied --gtf instead of using iGenomes one automatically def make_bed = false - if (!params.gene_bed) { + if (!gene_bed) { make_bed = true - } else if (params.genome && params.gtf) { - if (params.genomes[ params.genome ].gtf != params.gtf) { + } else if (genome && gtf) { + if (genomes[ genome ].gtf != gtf) { make_bed = true } } @@ -97,11 +108,11 @@ workflow PREPARE_GENOME { ch_gene_bed = GTF2BED ( ch_gtf ).bed ch_versions = ch_versions.mix(GTF2BED.out.versions) } else { - if (params.gene_bed.endsWith('.gz')) { - ch_gene_bed = GUNZIP_GENE_BED ( [ [:], params.gene_bed ] ).gunzip.map{ it[1] } + if (gene_bed.endsWith('.gz')) { + ch_gene_bed = GUNZIP_GENE_BED ( [ [:], gene_bed ] ).gunzip.map{ it[1] } ch_versions = ch_versions.mix(GUNZIP_GENE_BED.out.versions) } else { - ch_gene_bed = Channel.value(file(params.gene_bed)) + ch_gene_bed = Channel.value(file(gene_bed)) } } @@ -119,24 +130,23 @@ workflow PREPARE_GENOME { ch_genome_filtered_bed = Channel.empty() GENOME_BLACKLIST_REGIONS ( - CUSTOM_GETCHROMSIZES.out.sizes.map{ it[1] }, + ch_chrom_sizes, ch_blacklist.ifEmpty([]) ) ch_genome_filtered_bed = GENOME_BLACKLIST_REGIONS.out.bed ch_versions = ch_versions.mix(GENOME_BLACKLIST_REGIONS.out.versions) - // // Uncompress BWA index or generate from scratch if required // ch_bwa_index = Channel.empty() if (prepare_tool_index == 'bwa') { - if (params.bwa_index) { - if (params.bwa_index.endsWith('.tar.gz')) { - ch_bwa_index = UNTAR_BWA_INDEX ( [ [:], params.bwa_index ] ).untar + if (bwa_index) { + if (bwa_index.endsWith('.tar.gz')) { + ch_bwa_index = UNTAR_BWA_INDEX ( [ [:], bwa_index ] ).untar ch_versions = ch_versions.mix(UNTAR_BWA_INDEX.out.versions) } else { - ch_bwa_index = file(params.bwa_index) + ch_bwa_index = file(bwa_index) } } else { ch_bwa_index = BWA_INDEX ( ch_fasta.map { [ [:], it ] } ).index @@ -149,12 +159,12 @@ workflow PREPARE_GENOME { // ch_bowtie2_index = Channel.empty() if (prepare_tool_index == 'bowtie2') { - if (params.bowtie2_index) { - if (params.bowtie2_index.endsWith('.tar.gz')) { - ch_bowtie2_index = UNTAR_BOWTIE2_INDEX ( [ [:], params.bowtie2_index ] ).untar + if (bowtie2_index) { + if (bowtie2_index.endsWith('.tar.gz')) { + ch_bowtie2_index = UNTAR_BOWTIE2_INDEX ( [ [:], bowtie2_index ] ).untar ch_versions = ch_versions.mix(UNTAR_BOWTIE2_INDEX.out.versions) } else { - ch_bowtie2_index = [ [:], file(params.bowtie2_index) ] + ch_bowtie2_index = [ [:], file(bowtie2_index) ] } } else { ch_bowtie2_index = BOWTIE2_BUILD ( ch_fasta.map { [ [:], it ] } ).index @@ -167,12 +177,12 @@ workflow PREPARE_GENOME { // ch_chromap_index = Channel.empty() if (prepare_tool_index == 'chromap') { - if (params.chromap_index) { - if (params.chromap_index.endsWith('.tar.gz')) { - ch_chromap_index = UNTARFILES ( [ [:], params.chromap_index ] ).files + if (chromap_index) { + if (chromap_index.endsWith('.tar.gz')) { + ch_chromap_index = UNTARFILES ( [ [:], chromap_index ] ).files ch_versions = ch_versions.mix(UNTARFILES.out.versions) } else { - ch_chromap_index = [ [:], file(params.chromap_index) ] + ch_chromap_index = [ [:], file(chromap_index) ] } } else { ch_chromap_index = CHROMAP_INDEX ( ch_fasta.map { [ [:], it ] } ).index @@ -185,12 +195,12 @@ workflow PREPARE_GENOME { // ch_star_index = Channel.empty() if (prepare_tool_index == 'star') { - if (params.star_index) { - if (params.star_index.endsWith('.tar.gz')) { - ch_star_index = UNTAR_STAR_INDEX ( [ [:], params.star_index ] ).untar.map{ it[1] } + if (star_index) { + if (star_index.endsWith('.tar.gz')) { + ch_star_index = UNTAR_STAR_INDEX ( [ [:], star_index ] ).untar.map{ it[1] } ch_versions = ch_versions.mix(UNTAR_STAR_INDEX.out.versions) } else { - ch_star_index = Channel.value(file(params.star_index)) + ch_star_index = Channel.value(file(star_index)) } } else { ch_star_index = STAR_GENOMEGENERATE ( ch_fasta, ch_gtf ).index @@ -209,6 +219,5 @@ workflow PREPARE_GENOME { bowtie2_index = ch_bowtie2_index // path: bowtie2/index/ chromap_index = ch_chromap_index // path: genome.index star_index = ch_star_index // path: star/index/ - - versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] + versions = ch_versions.ifEmpty(null) // channel: [ versions.yml ] } From 2397708deb0082671378ae433119907028900090 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 14:53:32 +0200 Subject: [PATCH 149/201] Update main workflows --- main.nf | 134 ++++++++++++++++------- workflows/chipseq.nf | 248 ++++++++++++++++--------------------------- 2 files changed, 189 insertions(+), 193 deletions(-) diff --git a/main.nf b/main.nf index e9c7a38e..8ed7934d 100755 --- a/main.nf +++ b/main.nf @@ -13,72 +13,128 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') -params.bwa_index = WorkflowMain.getGenomeAttribute(params, 'bwa') -params.bowtie2_index = WorkflowMain.getGenomeAttribute(params, 'bowtie2') -params.chromap_index = WorkflowMain.getGenomeAttribute(params, 'chromap') -params.star_index = WorkflowMain.getGenomeAttribute(params, 'star') -params.gtf = WorkflowMain.getGenomeAttribute(params, 'gtf') -params.gff = WorkflowMain.getGenomeAttribute(params, 'gff') -params.gene_bed = WorkflowMain.getGenomeAttribute(params, 'gene_bed') -params.blacklist = WorkflowMain.getGenomeAttribute(params, 'blacklist') -params.macs_gsize = WorkflowMain.getMacsGsize(params) +include { CHIPSEQ } from './workflows/chipseq' +include { PREPARE_GENOME } from './subworkflows/local/prepare_genome' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +include { getMacsGsize } from './subworkflows/local/utils_nfcore_chipseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +params.fasta = getGenomeAttribute('fasta') +params.bwa_index = getGenomeAttribute('bwa') +params.bowtie2_index = getGenomeAttribute('bowtie2') +params.chromap_index = getGenomeAttribute('chromap') +params.star_index = getGenomeAttribute('star') +params.gtf = getGenomeAttribute('gtf') +params.gff = getGenomeAttribute('gff') +params.gene_bed = getGenomeAttribute('gene_bed') +params.blacklist = getGenomeAttribute('blacklist') +params.macs_gsize = getMacsGsize(params) /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { CHIPSEQ } from './workflows/chipseq' - // // WORKFLOW: Run main nf-core/chipseq analysis pipeline // workflow NFCORE_CHIPSEQ { - CHIPSEQ () + + main: + ch_versions = Channel.empty() + + // SUBWORKFLOW: Prepare genome files + PREPARE_GENOME ( + params.genome, + params.genomes, + params.aligner, + params.fasta, + params.gtf, + params.gff, + params.blacklist, + params.gene_bed, + params.bwa_index, + params.bowtie2_index, + params.chromap_index, + params.star_index, + ) + ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) + + // + // WORKFLOW: Run nf-core/chipseq workflow + // + ch_samplesheet = Channel.value(file(params.input, checkIfExists: true)) + + CHIPSEQ( + params.input, + ch_versions, + PREPARE_GENOME.out.fasta, + PREPARE_GENOME.out.fai, + PREPARE_GENOME.out.gtf, + PREPARE_GENOME.out.gene_bed, + PREPARE_GENOME.out.chrom_sizes, + PREPARE_GENOME.out.filtered_bed, + PREPARE_GENOME.out.bwa_index, + PREPARE_GENOME.out.bowtie2_index, + PREPARE_GENOME.out.chromap_index, + PREPARE_GENOME.out.star_index + ) + + emit: + multiqc_report = CHIPSEQSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [version1, version2, ...] } /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir + ) + + // + // WORKFLOW: Run main workflow + // NFCORE_CHIPSEQ () + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_CHIPSEQ.out.multiqc_report + ) } /* diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index f3f8e517..75a47387 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -1,61 +1,12 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -// Validate input parameters -WorkflowChipseq.initialise(params, log) - -ch_input = file(params.input) - -// Save AWS IGenomes file containing annotation version -def anno_readme = params.genomes[ params.genome ]?.readme -if (anno_readme && file(anno_readme).exists()) { - file("${params.outdir}/genome/").mkdirs() - file(anno_readme).copyTo("${params.outdir}/genome/") -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config ): Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -// JSON files required by BAMTools for alignment filtering -ch_bamtools_filter_se_config = file(params.bamtools_filter_se_config) -ch_bamtools_filter_pe_config = file(params.bamtools_filter_pe_config) - -// Header files for MultiQC -ch_spp_nsc_header = file("$projectDir/assets/multiqc/spp_nsc_header.txt", checkIfExists: true) -ch_spp_rsc_header = file("$projectDir/assets/multiqc/spp_rsc_header.txt", checkIfExists: true) -ch_spp_correlation_header = file("$projectDir/assets/multiqc/spp_correlation_header.txt", checkIfExists: true) -ch_peak_count_header = file("$projectDir/assets/multiqc/peak_count_header.txt", checkIfExists: true) -ch_frip_score_header = file("$projectDir/assets/multiqc/frip_score_header.txt", checkIfExists: true) -ch_peak_annotation_header = file("$projectDir/assets/multiqc/peak_annotation_header.txt", checkIfExists: true) -ch_deseq2_pca_header = file("$projectDir/assets/multiqc/deseq2_pca_header.txt", checkIfExists: true) -ch_deseq2_clustering_header = file("$projectDir/assets/multiqc/deseq2_clustering_header.txt", checkIfExists: true) - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ IMPORT LOCAL MODULES/SUBWORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ +// +// MODULE: Loaded from modules/local/ +// include { BEDTOOLS_GENOMECOV } from '../modules/local/bedtools_genomecov' include { FRIP_SCORE } from '../modules/local/frip_score' include { PLOT_MACS2_QC } from '../modules/local/plot_macs2_qc' @@ -71,10 +22,13 @@ include { MULTIQC_CUSTOM_PEAKS } from '../modules/local/multiqc_c // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' -include { PREPARE_GENOME } from '../subworkflows/local/prepare_genome' -include { ALIGN_STAR } from '../subworkflows/local/align_star' -include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_chipseq_pipeline' +include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { ALIGN_STAR } from '../subworkflows/local/align_star' +include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -98,7 +52,6 @@ include { DEEPTOOLS_PLOTFINGERPRINT } from '../modules/nf-core/deeptools/plo include { KHMER_UNIQUEKMERS } from '../modules/nf-core/khmer/uniquekmers/main' include { MACS2_CALLPEAK } from '../modules/nf-core/macs2/callpeak/main' include { SUBREAD_FEATURECOUNTS } from '../modules/nf-core/subread/featurecounts/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_MACS2 } from '../modules/nf-core/homer/annotatepeaks/main' include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../modules/nf-core/homer/annotatepeaks/main' @@ -119,26 +72,56 @@ include { BAM_MARKDUPLICATES_PICARD } from '../subworkflows/nf-core/bam_m ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] +// JSON files required by BAMTools for alignment filtering +ch_bamtools_filter_se_config = file(params.bamtools_filter_se_config) +ch_bamtools_filter_pe_config = file(params.bamtools_filter_pe_config) -workflow CHIPSEQ { +// Header files for MultiQC +ch_spp_nsc_header = file("$projectDir/assets/multiqc/spp_nsc_header.txt", checkIfExists: true) +ch_spp_rsc_header = file("$projectDir/assets/multiqc/spp_rsc_header.txt", checkIfExists: true) +ch_spp_correlation_header = file("$projectDir/assets/multiqc/spp_correlation_header.txt", checkIfExists: true) +ch_peak_count_header = file("$projectDir/assets/multiqc/peak_count_header.txt", checkIfExists: true) +ch_frip_score_header = file("$projectDir/assets/multiqc/frip_score_header.txt", checkIfExists: true) +ch_peak_annotation_header = file("$projectDir/assets/multiqc/peak_annotation_header.txt", checkIfExists: true) +ch_deseq2_pca_header = file("$projectDir/assets/multiqc/deseq2_pca_header.txt", checkIfExists: true) +ch_deseq2_clustering_header = file("$projectDir/assets/multiqc/deseq2_clustering_header.txt", checkIfExists: true) - ch_versions = Channel.empty() +// Save AWS IGenomes file containing annotation version +def anno_readme = params.genomes[ params.genome ]?.readme +if (anno_readme && file(anno_readme).exists()) { + file("${params.outdir}/genome/").mkdirs() + file(anno_readme).copyTo("${params.outdir}/genome/") +} - // - // SUBWORKFLOW: Uncompress and prepare reference genome files - // - PREPARE_GENOME ( - params.aligner - ) - ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) + +// // Info required for completion email and summary +// def multiqc_report = [] + +workflow CHIPSEQ { + + take: + ch_samplesheet // channel: path(sample_sheet.csv) + ch_versions // channel: [ path(versions.yml) ] + ch_fasta // channel: path(genome.fa) + ch_fai // channel: path(genome.fai) + ch_gtf // channel: path(genome.gtf) + ch_gene_bed // channel: path(gene.beds) + ch_chrom_sizes // channel: path(chrom.sizes) + ch_filtered_bed // channel: path(filtered.bed) + ch_bwa_index // channel: path(bwa/index/) + ch_bowtie2_index // channel: path(bowtie2/index) + ch_chromap_index // channel: path(chromap.index) + ch_star_index // channel: path(star/index/) + + main: + ch_multiqc_files = Channel.empty() // // SUBWORKFLOW: Read in samplesheet, validate and stage input files // + ch_input = file(ch_samplesheet) INPUT_CHECK ( - file(params.input), + ch_input, params.seq_center ) ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) @@ -171,13 +154,11 @@ workflow CHIPSEQ { if (params.aligner == 'bwa') { FASTQ_ALIGN_BWA ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, - PREPARE_GENOME.out.bwa_index, + ch_bwa_index, false, - PREPARE_GENOME - .out - .fasta + ch_fasta .map { - [ [:], it ] + [ [:], it ] } ) ch_genome_bam = FASTQ_ALIGN_BWA.out.bam @@ -194,12 +175,12 @@ workflow CHIPSEQ { if (params.aligner == 'bowtie2') { FASTQ_ALIGN_BOWTIE2 ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, - PREPARE_GENOME.out.bowtie2_index, + ch_bowtie2_index, params.save_unaligned, false, - PREPARE_GENOME.out.fasta + ch_fasta .map { - [ [:], it ] + [ [:], it ] } ) ch_genome_bam = FASTQ_ALIGN_BOWTIE2.out.bam @@ -216,10 +197,8 @@ workflow CHIPSEQ { if (params.aligner == 'chromap') { FASTQ_ALIGN_CHROMAP ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, - PREPARE_GENOME.out.chromap_index, - PREPARE_GENOME - .out - .fasta + ch_chromap_index, + ch_fasta .map { [ [:], it ] }, @@ -242,10 +221,8 @@ workflow CHIPSEQ { if (params.aligner == 'star') { ALIGN_STAR ( FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.reads, - PREPARE_GENOME.out.star_index, - PREPARE_GENOME - .out - .fasta + ch_star_index, + ch_fasta .map { [ [:], it ] }, @@ -290,13 +267,11 @@ workflow CHIPSEQ { // BAM_MARKDUPLICATES_PICARD ( PICARD_MERGESAMFILES.out.bam, - PREPARE_GENOME - .out - .fasta + ch_fasta .map { - [ [:], it ] + [ [:], it ] }, - PREPARE_GENOME.out.fai + ch_fai .map { [ [:], it ] } @@ -308,10 +283,8 @@ workflow CHIPSEQ { // BAM_FILTER_BAMTOOLS ( BAM_MARKDUPLICATES_PICARD.out.bam.join(BAM_MARKDUPLICATES_PICARD.out.bai, by: [0]), - PREPARE_GENOME.out.filtered_bed.first(), - PREPARE_GENOME - .out - .fasta + ch_filtered_bed.first(), + ch_fasta .map { [ [:], it ] }, @@ -344,15 +317,11 @@ workflow CHIPSEQ { .map { [ it[0], it[1], [] ] }, - PREPARE_GENOME - .out - .fasta + ch_fasta .map { [ [:], it ] }, - PREPARE_GENOME - .out - .fai + ch_fai .map { [ [:], it ] } @@ -402,7 +371,7 @@ workflow CHIPSEQ { // UCSC_BEDGRAPHTOBIGWIG ( BEDTOOLS_GENOMECOV.out.bedgraph, - PREPARE_GENOME.out.chrom_sizes + ch_chrom_sizes ) ch_versions = ch_versions.mix(UCSC_BEDGRAPHTOBIGWIG.out.versions.first()) @@ -413,7 +382,7 @@ workflow CHIPSEQ { // DEEPTOOLS_COMPUTEMATRIX ( UCSC_BEDGRAPHTOBIGWIG.out.bigwig, - PREPARE_GENOME.out.gene_bed + ch_gene_bed ) ch_versions = ch_versions.mix(DEEPTOOLS_COMPUTEMATRIX.out.versions.first()) @@ -475,6 +444,7 @@ workflow CHIPSEQ { // // MODULE: Calculute genome size with khmer // + // TODO move to prepare genome ch_macs_gsize = Channel.empty() ch_custompeaks_frip_multiqc = Channel.empty() ch_custompeaks_count_multiqc = Channel.empty() @@ -483,7 +453,7 @@ workflow CHIPSEQ { ch_macs_gsize = params.macs_gsize if (!params.macs_gsize) { KHMER_UNIQUEKMERS ( - PREPARE_GENOME.out.fasta, + ch_fasta, params.read_length ) ch_macs_gsize = KHMER_UNIQUEKMERS.out.kmers.map { it.text.trim() } @@ -561,8 +531,8 @@ workflow CHIPSEQ { // HOMER_ANNOTATEPEAKS_MACS2 ( ch_macs2_peaks, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.gtf + ch_fasta, + ch_gtf ) ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS_MACS2.out.versions.first()) @@ -640,8 +610,8 @@ workflow CHIPSEQ { // HOMER_ANNOTATEPEAKS_CONSENSUS ( MACS2_CONSENSUS.out.bed, - PREPARE_GENOME.out.fasta, - PREPARE_GENOME.out.gtf + ch_fasta, + ch_gtf ) ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS_CONSENSUS.out.versions) @@ -708,7 +678,7 @@ workflow CHIPSEQ { IGV ( params.aligner, params.narrow_peak ? 'narrow_peak' : 'broad_peak', - PREPARE_GENOME.out.fasta, + ch_fasta, UCSC_BEDGRAPHTOBIGWIG.out.bigwig.collect{it[1]}.ifEmpty([]), ch_macs2_peaks.collect{it[1]}.ifEmpty([]), ch_macs2_consensus_bed_lib.collect{it[1]}.ifEmpty([]), @@ -718,29 +688,29 @@ workflow CHIPSEQ { } // - // MODULE: Pipeline reporting + // Collate and save software versions // - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_atacseq_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // if (!params.skip_multiqc) { - workflow_summary = WorkflowChipseq.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowChipseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config ): Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo ) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) MULTIQC ( - ch_multiqc_config, - ch_multiqc_custom_config.collect().ifEmpty([]), - CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect(), - ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml'), - ch_methods_description.collectFile(name: 'methods_description_mqc.yaml'), - ch_multiqc_logo.collect().ifEmpty([]), + ch_multiqc_files.collect(), + ch_multiqc_config.toList(), + ch_multiqc_custom_config.toList(), + ch_multiqc_logo.toList(), FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.fastqc_zip.collect{it[1]}.ifEmpty([]), FASTQ_FASTQC_UMITOOLS_TRIMGALORE.out.trim_zip.collect{it[1]}.ifEmpty([]), @@ -778,36 +748,6 @@ workflow CHIPSEQ { ch_deseq2_pca_multiqc.collect().ifEmpty([]), ch_deseq2_clustering_multiqc.collect().ifEmpty([]) ) - multiqc_report = MULTIQC.out.report.toList() - } -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} - -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + ch_multiqc_report = MULTIQC.out.report } } - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - THE END -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ From 9d926f74375335af3e7a29bb87991bb6cdc20d96 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 15:27:18 +0200 Subject: [PATCH 150/201] Add emit --- main.nf | 2 +- workflows/chipseq.nf | 10 ++++++++++ 2 files changed, 11 insertions(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 8ed7934d..1eaaca40 100755 --- a/main.nf +++ b/main.nf @@ -92,7 +92,7 @@ workflow NFCORE_CHIPSEQ { ) emit: - multiqc_report = CHIPSEQSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html + multiqc_report = CHIPSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html versions = ch_versions // channel: [version1, version2, ...] } diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 75a47387..91295716 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -750,4 +750,14 @@ workflow CHIPSEQ { ) ch_multiqc_report = MULTIQC.out.report } + + emit: + multiqc_report = ch_multiqc_report // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + THE END +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ \ No newline at end of file From 4c2cce86af085deebb41c131d666d2218da6e2a6 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 15:31:21 +0200 Subject: [PATCH 151/201] Align comment --- main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/main.nf b/main.nf index 1eaaca40..cb61186e 100755 --- a/main.nf +++ b/main.nf @@ -93,7 +93,7 @@ workflow NFCORE_CHIPSEQ { emit: multiqc_report = CHIPSEQ.out.multiqc_report // channel: /path/to/multiqc_report.html - versions = ch_versions // channel: [version1, version2, ...] + versions = ch_versions // channel: [version1, version2, ...] } /* From 69ed785cbfa04be9ead80151dff334f7bd68c511 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 15:36:50 +0200 Subject: [PATCH 152/201] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index b936b306..1d0102de 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -23,6 +23,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated pipeline template to [nf-core/tools 2.10](https://github.com/nf-core/tools/releases/tag/2.10). - [[#367](https://github.com/nf-core/chipseq/issues/367)] - Get rid of `CheckIfExists` for params paths. - [[#370](https://github.com/nf-core/chipseq/issues/370)] - Fix stack overflow exceptions in phantompeakqualtools ([see here](https://github.com/kundajelab/phantompeakqualtools/issues/3)). +- [[PR #391](https://github.com/nf-core/chipseq/pull/391)] - Get rid of the `lib` folder and rearrange the pipeline accordingly. ### Software dependencies From a1b8e00f775d9d550302d5fbb8abd7b803afc8fe Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 26 Jun 2024 15:49:22 +0200 Subject: [PATCH 153/201] Make lint happy --- conf/base.config | 3 --- main.nf | 2 +- subworkflows/local/prepare_genome.nf | 4 ++-- subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf | 2 +- workflows/chipseq.nf | 2 +- 5 files changed, 5 insertions(+), 8 deletions(-) diff --git a/conf/base.config b/conf/base.config index b5fcc4af..42ba5a89 100644 --- a/conf/base.config +++ b/conf/base.config @@ -57,7 +57,4 @@ process { errorStrategy = 'retry' maxRetries = 2 } - withName:CUSTOM_DUMPSOFTWAREVERSIONS { - cache = false - } } diff --git a/main.nf b/main.nf index cb61186e..c0623ffd 100755 --- a/main.nf +++ b/main.nf @@ -70,7 +70,7 @@ workflow NFCORE_CHIPSEQ { params.star_index, ) ch_versions = ch_versions.mix(PREPARE_GENOME.out.versions) - + // // WORKFLOW: Run nf-core/chipseq workflow // diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index f3bc02eb..b7878aaf 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -39,11 +39,11 @@ workflow PREPARE_GENOME { bowtie2_index // file: /path/to/bowtie2/index/ chromap_index // file: /path/to/chromap/index/ star_index // file: /path/to/star/index/ - + main: ch_versions = Channel.empty() - + // // Uncompress genome fasta file if required // diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf index 92daace0..a1433174 100644 --- a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -265,4 +265,4 @@ def macsGsizeWarn(log) { " It will be auto-calculated by 'khmer unique-kmers.py' using the '--read_length' parameter.\n" + " Explicitly provide '--macs_gsize macs2_genome_size' to change this behaviour.\n" + "===================================================================================" -} \ No newline at end of file +} diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 91295716..1677ad3a 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -112,7 +112,7 @@ workflow CHIPSEQ { ch_bowtie2_index // channel: path(bowtie2/index) ch_chromap_index // channel: path(chromap.index) ch_star_index // channel: path(star/index/) - + main: ch_multiqc_files = Channel.empty() From 5bf333dd4553962bf2b641f400681182b4a8708c Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 27 Jun 2024 22:22:26 +0200 Subject: [PATCH 154/201] Make lint happy --- workflows/chipseq.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 1677ad3a..cae508d6 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -760,4 +760,4 @@ workflow CHIPSEQ { ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ \ No newline at end of file +*/ From 688c319dde63dfd9d26acb0ed40b31ce88021391 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 27 Jun 2024 22:22:49 +0200 Subject: [PATCH 155/201] Update phantompeakqualtools --- modules.json | 195 +++++------------- .../phantompeakqualtools/environment.yml | 9 + modules/nf-core/phantompeakqualtools/main.nf | 20 +- modules/nf-core/phantompeakqualtools/meta.yml | 13 +- .../phantompeakqualtools/tests/main.nf.test | 90 ++++++++ .../tests/main.nf.test.snap | 119 +++++++++++ .../phantompeakqualtools/tests/tags.yml | 2 + 7 files changed, 289 insertions(+), 159 deletions(-) create mode 100644 modules/nf-core/phantompeakqualtools/environment.yml create mode 100644 modules/nf-core/phantompeakqualtools/tests/main.nf.test create mode 100644 modules/nf-core/phantompeakqualtools/tests/main.nf.test.snap create mode 100644 modules/nf-core/phantompeakqualtools/tests/tags.yml diff --git a/modules.json b/modules.json index 285d38b9..14d352e9 100644 --- a/modules.json +++ b/modules.json @@ -8,252 +8,172 @@ "bowtie2/align": { "branch": "master", "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", - "installed_by": [ - "fastq_align_bowtie2", - "modules" - ] + "installed_by": ["fastq_align_bowtie2", "modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", - "installed_by": [ - "fastq_align_bwa", - "modules" - ] + "installed_by": ["fastq_align_bwa", "modules"] }, "chromap/chromap": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "fastq_align_chromap", - "modules" - ] + "installed_by": ["fastq_align_chromap", "modules"] }, "chromap/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/getchromsizes": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/computematrix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "gffread": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "macs2/callpeak": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "phantompeakqualtools": { "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "git_sha": "2dfe9afa90fefc70e320140e5f41287f01f324b0", + "installed_by": ["modules"] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/markduplicates": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": [ - "bam_markduplicates_picard", - "modules" - ] + "installed_by": ["bam_markduplicates_picard", "modules"] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "preseq/lcextrap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/idxstats": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/index": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_markduplicates_picard", - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools", "modules"] }, "samtools/sort": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_sort_stats_samtools", "modules"] }, "samtools/stats": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "subread/featurecounts": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "trimgalore": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "umitools/extract": { "branch": "master", "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore"] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "untarfiles": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -262,78 +182,55 @@ "bam_markduplicates_picard": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "bam_sort_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "fastq_align_bowtie2", - "fastq_align_bwa", - "fastq_align_chromap" - ] + "installed_by": ["fastq_align_bowtie2", "fastq_align_bwa", "fastq_align_chromap"] }, "bam_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "bam_markduplicates_picard", - "bam_sort_stats_samtools" - ] + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_bwa": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_chromap": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } } } -} \ No newline at end of file +} diff --git a/modules/nf-core/phantompeakqualtools/environment.yml b/modules/nf-core/phantompeakqualtools/environment.yml new file mode 100644 index 00000000..095b7b4b --- /dev/null +++ b/modules/nf-core/phantompeakqualtools/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "phantompeakqualtools" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::phantompeakqualtools=1.2.2" diff --git a/modules/nf-core/phantompeakqualtools/main.nf b/modules/nf-core/phantompeakqualtools/main.nf index edcdf2ce..f0f7dc63 100644 --- a/modules/nf-core/phantompeakqualtools/main.nf +++ b/modules/nf-core/phantompeakqualtools/main.nf @@ -3,10 +3,10 @@ process PHANTOMPEAKQUALTOOLS { label 'process_medium' // WARN: Version information not provided by tool on CLI. Please update version string below when bumping container versions. - conda "bioconda::phantompeakqualtools=1.2.2" + conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/phantompeakqualtools:1.2.2--0' : - 'biocontainers/phantompeakqualtools:1.2.2--0' }" + 'oras://community.wave.seqera.io/library/phantompeakqualtools:1.2.2--50be7727b2a72700' : + 'community.wave.seqera.io/library/phantompeakqualtools:1.2.2--f8026fe2526a5e18' }" input: tuple val(meta), path(bam) @@ -34,4 +34,18 @@ process PHANTOMPEAKQUALTOOLS { phantompeakqualtools: $VERSION END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '1.2.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. + """ + touch ${prefix}.spp.pdf + touch ${prefix}.spp.Rdata + touch ${prefix}.spp.out + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + phantompeakqualtools: $VERSION + END_VERSIONS + """ } diff --git a/modules/nf-core/phantompeakqualtools/meta.yml b/modules/nf-core/phantompeakqualtools/meta.yml index c8290fbd..276f6fdd 100644 --- a/modules/nf-core/phantompeakqualtools/meta.yml +++ b/modules/nf-core/phantompeakqualtools/meta.yml @@ -1,5 +1,4 @@ name: "phantompeakqualtools" - description: | "This package computes informative enrichment and quality measures for ChIP-seq/DNase-seq/FAIRE-seq/MNase-seq data. It can also be used @@ -16,12 +15,10 @@ tools: for ChIP-seq/DNase-seq/FAIRE-seq/MNase-seq data. It can also be used to obtain robust estimates of the predominant fragment length or characteristic tag shift values in these assays." - documentation: "https://github.com/kundajelab/phantompeakqualtools" tool_dev_url: "https://github.com/kundajelab/phantompeakqualtools" doi: "10.1101/gr.136184.111" - licence: "['BSD-3-clause']" - + licence: ["BSD-3-clause"] input: - meta: type: map @@ -32,7 +29,6 @@ input: type: file description: BAM/CRAM/SAM file pattern: "*.{bam,cram,sam}" - output: - meta: type: map @@ -57,8 +53,11 @@ output: type: file description: Rdata file containing the R session pattern: "*.{Rdata}" - authors: - "@drpatelh" - - "@Emiller88" + - "@edmundmiller" + - "@JoseEspinosa" +maintainers: + - "@drpatelh" + - "@edmundmiller" - "@JoseEspinosa" diff --git a/modules/nf-core/phantompeakqualtools/tests/main.nf.test b/modules/nf-core/phantompeakqualtools/tests/main.nf.test new file mode 100644 index 00000000..ea096bcf --- /dev/null +++ b/modules/nf-core/phantompeakqualtools/tests/main.nf.test @@ -0,0 +1,90 @@ +// nf-core modules test phantompeakqualtools +nextflow_process { + + name "Test Process PHANTOMPEAKQUALTOOLS" + script "../main.nf" + process "PHANTOMPEAKQUALTOOLS" + + tag "modules" + tag "modules_nfcore" + tag "phantompeakqualtools" + + test("sarscov2 - bam - single_end") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.single_end.bam', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.spp, + file(process.out.pdf.get(0).get(1)).name, + file(process.out.rdata.get(0).get(1)).name, + process.out.versions) + .match() + } + ) + } + + } + + test("sarscov2 - bam - paired_end") { + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.spp, + file(process.out.pdf.get(0).get(1)).name, + file(process.out.rdata.get(0).get(1)).name, + process.out.versions) + .match() + } + ) + } + + } + + test("sarscov2 - bam - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} \ No newline at end of file diff --git a/modules/nf-core/phantompeakqualtools/tests/main.nf.test.snap b/modules/nf-core/phantompeakqualtools/tests/main.nf.test.snap new file mode 100644 index 00000000..1021aeb8 --- /dev/null +++ b/modules/nf-core/phantompeakqualtools/tests/main.nf.test.snap @@ -0,0 +1,119 @@ +{ + "sarscov2 - bam - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.spp.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.spp.pdf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test", + "single_end": false + }, + "test.spp.Rdata:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,e0a48a40af2cf7d5de72c4c3cb47a4fc" + ], + "pdf": [ + [ + { + "id": "test", + "single_end": false + }, + "test.spp.pdf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "rdata": [ + [ + { + "id": "test", + "single_end": false + }, + "test.spp.Rdata:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "spp": [ + [ + { + "id": "test", + "single_end": false + }, + "test.spp.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e0a48a40af2cf7d5de72c4c3cb47a4fc" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-27T10:46:22.786363" + }, + "sarscov2 - bam - single_end": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.spp.out:md5,b01d976506b6fe45b66c821b1e8a1d15" + ] + ], + "test.spp.pdf", + "test.spp.Rdata", + [ + "versions.yml:md5,e0a48a40af2cf7d5de72c4c3cb47a4fc" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-27T16:21:12.000709154" + }, + "sarscov2 - bam - paired_end": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.spp.out:md5,eed46e75eab119224f397a7a8b5924e6" + ] + ], + "test.spp.pdf", + "test.spp.Rdata", + [ + "versions.yml:md5,e0a48a40af2cf7d5de72c4c3cb47a4fc" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-06-27T16:21:22.432426907" + } +} \ No newline at end of file diff --git a/modules/nf-core/phantompeakqualtools/tests/tags.yml b/modules/nf-core/phantompeakqualtools/tests/tags.yml new file mode 100644 index 00000000..9031749e --- /dev/null +++ b/modules/nf-core/phantompeakqualtools/tests/tags.yml @@ -0,0 +1,2 @@ +phantompeakqualtools: + - "modules/nf-core/phantompeakqualtools/**" From f85bcd7e5a05060c85e6617392c8a4a3ee2a03ad Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 27 Jun 2024 23:00:31 +0200 Subject: [PATCH 156/201] Use wave container to avoid docker format version error --- modules/local/multiqc_custom_phantompeakqualtools.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/local/multiqc_custom_phantompeakqualtools.nf b/modules/local/multiqc_custom_phantompeakqualtools.nf index c9239bde..7fc74a2e 100644 --- a/modules/local/multiqc_custom_phantompeakqualtools.nf +++ b/modules/local/multiqc_custom_phantompeakqualtools.nf @@ -1,9 +1,9 @@ process MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS { tag "$meta.id" - conda "conda-forge::r-base=3.5.1" + conda "conda-forge::r-base=4.3.3" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/r-base:3.5.1': - 'biocontainers/r-base:3.5.1' }" + 'oras://community.wave.seqera.io/library/r-base:4.3.3--452dec8277637366': + 'community.wave.seqera.io/library/r-base:4.3.3--14bb33ac537aea22' }" input: tuple val(meta), path(spp), path(rdata) From 386217063fe4cd348d80dfd1ff1a00e83778e9e1 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Fri, 28 Jun 2024 10:21:14 +0200 Subject: [PATCH 157/201] fix nf-core lint --- .nf-core.yml | 5 +++++ 1 file changed, 5 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 2e90e7d0..3f08fd2d 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -5,3 +5,8 @@ lint: - config_defaults: - params.bamtools_filter_se_config - params.bamtools_filter_pe_config + files_unchanged: + - .github/CONTRIBUTING.md + - .github/workflows/branch.yml + - .github/workflows/linting_comment.yml + - .github/workflows/linting.yml From 259485dec273c39ade0b56e61879b00fcdca5e66 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 2 Jul 2024 09:38:55 +0200 Subject: [PATCH 158/201] Fix lint --- modules.json | 56 +--------------------------------------------------- 1 file changed, 1 insertion(+), 55 deletions(-) diff --git a/modules.json b/modules.json index 9aef55a4..812c7ed8 100644 --- a/modules.json +++ b/modules.json @@ -62,7 +62,7 @@ }, "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "gffread": { @@ -177,60 +177,6 @@ } } }, - "subworkflows": { - "nf-core": { - "bam_markduplicates_picard": { - "branch": "master", - "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["subworkflows"] - }, - "bam_sort_stats_samtools": { - "branch": "master", - "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["fastq_align_bowtie2", "fastq_align_bwa", "fastq_align_chromap"] - }, - "bam_stats_samtools": { - "branch": "master", - "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] - }, - "fastq_align_bowtie2": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - }, - "fastq_align_bwa": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - }, - "fastq_align_chromap": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - }, - "fastq_fastqc_umitools_trimgalore": { - "branch": "master", - "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] - }, - "utils_nextflow_pipeline": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - }, - "utils_nfcore_pipeline": { - "branch": "master", - "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", - "installed_by": ["subworkflows"] - }, - "utils_nfvalidation_plugin": { - "branch": "master", - "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] - } - } - }, "subworkflows": { "nf-core": { "utils_nextflow_pipeline": { From 9b646fecff06c6cb0e1ea9d61d1ea5ab98bfce15 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 2 Jul 2024 10:30:53 +0200 Subject: [PATCH 159/201] Remove files_unchanged after template update --- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .nf-core.yml | 5 ----- 2 files changed, 1 insertion(+), 6 deletions(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 4cd6ca4f..2f34f87d 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/chip - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/chipseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/chipseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. -- [ ] Make sure your code lints (`nf-core lint`). +- [ ] Make sure your code lints (`nf-core pipelines lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. diff --git a/.nf-core.yml b/.nf-core.yml index e2500bf9..add014ed 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -5,8 +5,3 @@ lint: - config_defaults: - params.bamtools_filter_se_config - params.bamtools_filter_pe_config - files_unchanged: - - .github/CONTRIBUTING.md - - .github/workflows/branch.yml - - .github/workflows/linting_comment.yml - - .github/workflows/linting.yml From 8ebded6006640e6d7de82aacfdfbad1d3918ed69 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 2 Jul 2024 10:39:21 +0200 Subject: [PATCH 160/201] Roll back file to template version v2.14.1 and not dev --- .github/PULL_REQUEST_TEMPLATE.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 2f34f87d..4cd6ca4f 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -17,7 +17,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/chip - [ ] If you've fixed a bug or added code that should be tested, add tests! - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/chipseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/chipseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. -- [ ] Make sure your code lints (`nf-core pipelines lint`). +- [ ] Make sure your code lints (`nf-core lint`). - [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. From db32e8bceecbccf30fa0c127d02849dfc9c46011 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 2 Jul 2024 10:59:46 +0200 Subject: [PATCH 161/201] Bring back subworkflows in modules.json deleted while resolving merge conflicts --- modules.json | 35 +++++++++++++++++++++++++++++++++++ 1 file changed, 35 insertions(+) diff --git a/modules.json b/modules.json index 812c7ed8..f4d4950b 100644 --- a/modules.json +++ b/modules.json @@ -179,6 +179,41 @@ }, "subworkflows": { "nf-core": { + "bam_markduplicates_picard": { + "branch": "master", + "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", + "installed_by": ["subworkflows"] + }, + "bam_sort_stats_samtools": { + "branch": "master", + "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", + "installed_by": ["fastq_align_bowtie2", "fastq_align_bwa", "fastq_align_chromap"] + }, + "bam_stats_samtools": { + "branch": "master", + "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] + }, + "fastq_align_bowtie2": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + }, + "fastq_align_bwa": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + }, + "fastq_align_chromap": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + }, + "fastq_fastqc_umitools_trimgalore": { + "branch": "master", + "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", + "installed_by": ["subworkflows"] + }, "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", From c0c63478ef4628f6abfb85c294dcd533044eca6b Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 3 Jul 2024 10:43:03 +0200 Subject: [PATCH 162/201] Fix changelog --- CHANGELOG.md | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 1d0102de..b5630f67 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -23,7 +23,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Updated pipeline template to [nf-core/tools 2.10](https://github.com/nf-core/tools/releases/tag/2.10). - [[#367](https://github.com/nf-core/chipseq/issues/367)] - Get rid of `CheckIfExists` for params paths. - [[#370](https://github.com/nf-core/chipseq/issues/370)] - Fix stack overflow exceptions in phantompeakqualtools ([see here](https://github.com/kundajelab/phantompeakqualtools/issues/3)). -- [[PR #391](https://github.com/nf-core/chipseq/pull/391)] - Get rid of the `lib` folder and rearrange the pipeline accordingly. +- [[#387](https://github.com/nf-core/chipseq/issues/387)] - Get rid of the `lib` folder and rearrange the pipeline accordingly. ### Software dependencies From a628cc467441419aeabb56514c2505b5e8d723a3 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 3 Jul 2024 10:43:40 +0200 Subject: [PATCH 163/201] Fix save_unaligned parameter description in schema --- CHANGELOG.md | 1 + nextflow_schema.json | 12 +++++++++++- 2 files changed, 12 insertions(+), 1 deletion(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index b5630f67..5677e847 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -24,6 +24,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#367](https://github.com/nf-core/chipseq/issues/367)] - Get rid of `CheckIfExists` for params paths. - [[#370](https://github.com/nf-core/chipseq/issues/370)] - Fix stack overflow exceptions in phantompeakqualtools ([see here](https://github.com/kundajelab/phantompeakqualtools/issues/3)). - [[#387](https://github.com/nf-core/chipseq/issues/387)] - Get rid of the `lib` folder and rearrange the pipeline accordingly. +- [[#385](https://github.com/nf-core/chipseq/issues/385)] - Fix `--save_unaligned` description in schema. ### Software dependencies diff --git a/nextflow_schema.json b/nextflow_schema.json index 668e4c4d..b0e6317c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -261,7 +261,7 @@ "save_unaligned": { "type": "boolean", "fa_icon": "fas fa-save", - "description": "Where possible, save unaligned reads from either STAR, HISAT2 or Salmon to the results directory.", + "description": "Save unaligned sequences to the output directory (only available for Bowtie 2 And STAR.", "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." }, "bamtools_filter_pe_config": { @@ -399,6 +399,16 @@ "description": "Skip MultiQC.", "fa_icon": "fas fa-fast-forward" }, + "skip_peak_calling": { + "type": "boolean", + "description": "Skip MACS2 peak calling.", + "fa_icon": "fas fa-fast-forward" + }, + "skip_reporting": { + "type": "boolean", + "description": "Skip reporting.", + "fa_icon": "fas fa-fast-forward" + }, "skip_qc": { "type": "boolean", "fa_icon": "fas fa-fast-forward", From f6b9c2707ba4b4ac677034e862c078fa20674d9e Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 3 Jul 2024 11:16:17 +0200 Subject: [PATCH 164/201] Remove code leak --- nextflow_schema.json | 10 ---------- 1 file changed, 10 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index b0e6317c..b7c4e106 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -399,16 +399,6 @@ "description": "Skip MultiQC.", "fa_icon": "fas fa-fast-forward" }, - "skip_peak_calling": { - "type": "boolean", - "description": "Skip MACS2 peak calling.", - "fa_icon": "fas fa-fast-forward" - }, - "skip_reporting": { - "type": "boolean", - "description": "Skip reporting.", - "fa_icon": "fas fa-fast-forward" - }, "skip_qc": { "type": "boolean", "fa_icon": "fas fa-fast-forward", From ac7feeec2c4fd02fc3c830019808486c1cbe2da8 Mon Sep 17 00:00:00 2001 From: Jose Espinosa-Carrasco Date: Wed, 3 Jul 2024 11:28:38 +0200 Subject: [PATCH 165/201] Update nextflow_schema.json MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit Co-authored-by: Matthias Hörtenhuber --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index b7c4e106..f51e0940 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -261,7 +261,7 @@ "save_unaligned": { "type": "boolean", "fa_icon": "fas fa-save", - "description": "Save unaligned sequences to the output directory (only available for Bowtie 2 And STAR.", + "description": "Save unaligned sequences to the output directory (only available for Bowtie 2 and STAR.", "help_text": "This may either be in the form of FastQ or BAM files depending on the options available for that particular tool." }, "bamtools_filter_pe_config": { From 80a12ace5266de5529f0e84d633991a9550e7389 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Steffen=20M=C3=B6ller?= Date: Mon, 1 Jul 2024 14:35:49 +0200 Subject: [PATCH 166/201] check: Adding line number to error messages. Co-authored-by: Jose Espinosa-Carrasco --- CHANGELOG.md | 1 + bin/check_samplesheet.py | 28 ++++++++++++++-------------- 2 files changed, 15 insertions(+), 14 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 5677e847..777d00b5 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#370](https://github.com/nf-core/chipseq/issues/370)] - Fix stack overflow exceptions in phantompeakqualtools ([see here](https://github.com/kundajelab/phantompeakqualtools/issues/3)). - [[#387](https://github.com/nf-core/chipseq/issues/387)] - Get rid of the `lib` folder and rearrange the pipeline accordingly. - [[#385](https://github.com/nf-core/chipseq/issues/385)] - Fix `--save_unaligned` description in schema. +- [[PR #392](https://github.com/nf-core/chipseq/pull/392)] - Adding line numbers to warnings/errors messages in `bin/check_samplesheet.py` ### Software dependencies diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 7005bb9e..36351a71 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -58,22 +58,22 @@ def check_samplesheet(file_in, file_out): sys.exit(1) ## Check sample entries - for line in fin: + for line_number, line in enumerate(fin, start=1): if line.strip(): lspl = [x.strip().strip('"') for x in line.strip().split(",")] # Check valid number of columns per row if len(lspl) < len(HEADER): print_error( - "Invalid number of columns (minimum = {})!".format(len(HEADER)), - "Line", + "Invalid number of columns (found = {}, minimum = {})!".format(len(lspl),len(HEADER)), + "Line {}".format(line_number), line, ) num_cols = len([x for x in lspl[: len(HEADER)] if x]) if num_cols < MIN_COLS: print_error( - "Invalid number of populated columns (minimum = {})!".format(MIN_COLS), - "Line", + "Invalid number of populated columns (found = {}, minimum = {})!".format(num_cols,MIN_COLS), + "Line {}".format(line_number), line, ) @@ -83,23 +83,23 @@ def check_samplesheet(file_in, file_out): print(f"WARNING: Spaces have been replaced by underscores for sample: {sample}") sample = sample.replace(" ", "_") if not sample: - print_error("Sample entry has not been specified!", "Line", line) + print_error("Sample entry has not been specified!", "Line {}".format(line_number), line) ## Check FastQ file extension for fastq in [fastq_1, fastq_2]: if fastq: if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", "Line", line) + print_error("FastQ file contains spaces!", "Line {}".format(lineNo), line) if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): print_error( "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", - "Line", + "Line {}".format(lineNo), line, ) ## Check replicate column is integer if not replicate.isdecimal(): - print_error("Replicate id not an integer!", "Line", line) + print_error("Replicate id not an integer!", "Line {}".format(lineNo), line) sys.exit(1) ## Check antibody and control columns have valid values @@ -110,7 +110,7 @@ def check_samplesheet(file_in, file_out): if not control: print_error( "Both antibody and control columns must be specified!", - "Line", + "Line {}".format(line_number), line, ) @@ -119,13 +119,13 @@ def check_samplesheet(file_in, file_out): print(f"WARNING: Spaces have been replaced by underscores for control: {control}") control = control.replace(" ", "_") if not control_replicate.isdecimal(): - print_error("Control replicate id not an integer!", "Line", line) + print_error("Control replicate id not an integer!", "Line {}".format(line_number), line) sys.exit(1) control = "{}_REP{}".format(control, control_replicate) if not antibody: print_error( "Both antibody and control columns must be specified!", - "Line", + "Line {}".format(line_number), line, ) @@ -138,7 +138,7 @@ def check_samplesheet(file_in, file_out): elif sample and fastq_1 and not fastq_2: sample_info = ["1", fastq_1, fastq_2, replicate, antibody, control] else: - print_error("Invalid combination of columns provided!", "Line", line) + print_error("Invalid combination of columns provided!", "Line {}".format(line_number), line) ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, replicate, antibody, control ]]} replicate = int(replicate) @@ -149,7 +149,7 @@ def check_samplesheet(file_in, file_out): sample_mapping_dict[sample][replicate] = [sample_info] else: if sample_info in sample_mapping_dict[sample][replicate]: - print_error("Samplesheet contains duplicate rows!", "Line", line) + print_error("Samplesheet contains duplicate rows!", "Line {}".format(line_number), line) else: sample_mapping_dict[sample][replicate].append(sample_info) From e7fba4870cde9b676a6f4446fdb5a411dade8185 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 4 Jul 2024 14:12:20 +0200 Subject: [PATCH 167/201] Check for sample IDs allowed characters --- CHANGELOG.md | 3 ++- bin/check_samplesheet.py | 13 ++++++++++--- 2 files changed, 12 insertions(+), 4 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 777d00b5..be4590e9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -25,7 +25,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#370](https://github.com/nf-core/chipseq/issues/370)] - Fix stack overflow exceptions in phantompeakqualtools ([see here](https://github.com/kundajelab/phantompeakqualtools/issues/3)). - [[#387](https://github.com/nf-core/chipseq/issues/387)] - Get rid of the `lib` folder and rearrange the pipeline accordingly. - [[#385](https://github.com/nf-core/chipseq/issues/385)] - Fix `--save_unaligned` description in schema. -- [[PR #392](https://github.com/nf-core/chipseq/pull/392)] - Adding line numbers to warnings/errors messages in `bin/check_samplesheet.py` +- [[PR #392](https://github.com/nf-core/chipseq/pull/392)] - Adding line numbers to warnings/errors messages in `bin/check_samplesheet.py`. +- [[#396](https://github.com/nf-core/chipseq/issues/396)] - Check that samplesheet samples IDs do only have alphanumeric characters, dots, dashes or underscores. ### Software dependencies diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py index 36351a71..c7d8add7 100755 --- a/bin/check_samplesheet.py +++ b/bin/check_samplesheet.py @@ -4,6 +4,7 @@ import sys import errno import argparse +import re def parse_args(args=None): @@ -84,22 +85,28 @@ def check_samplesheet(file_in, file_out): sample = sample.replace(" ", "_") if not sample: print_error("Sample entry has not been specified!", "Line {}".format(line_number), line) + if not re.match(r"^[a-zA-Z0-9_.-]+$", sample): + print_error( + "Sample name contains invalid characters! Only alphanumeric characters, underscores, dots and dashes are allowed.", + "Line {}".format(line_number), + line, + ) ## Check FastQ file extension for fastq in [fastq_1, fastq_2]: if fastq: if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", "Line {}".format(lineNo), line) + print_error("FastQ file contains spaces!", "Line {}".format(line_number), line) if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"): print_error( "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", - "Line {}".format(lineNo), + "Line {}".format(line_number), line, ) ## Check replicate column is integer if not replicate.isdecimal(): - print_error("Replicate id not an integer!", "Line {}".format(lineNo), line) + print_error("Replicate id not an integer!", "Line {}".format(line_number), line) sys.exit(1) ## Check antibody and control columns have valid values From f170418ff3d3ce11cb446cc405eb26e526357014 Mon Sep 17 00:00:00 2001 From: Bjorn Langer Date: Thu, 4 Jul 2024 18:44:54 +0200 Subject: [PATCH 168/201] use subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc from nf-core/atacseq --- .../bam_bedgraph_bigwig_bedtools_ucsc.nf | 42 +++++++++++++++++++ 1 file changed, 42 insertions(+) create mode 100644 subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf diff --git a/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf b/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf new file mode 100644 index 00000000..0947571c --- /dev/null +++ b/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf @@ -0,0 +1,42 @@ + +// +// Convert BAM to normalised bigWig via bedGraph using BEDTools and UCSC +// + +include { BEDTOOLS_GENOMECOV } from '../../modules/local/bedtools_genomecov' +include { UCSC_BEDGRAPHTOBIGWIG } from '../../modules/nf-core/ucsc/bedgraphtobigwig/main' + +workflow BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC { + take: + ch_bam_flagstat // channel: [ val(meta), [bam], [flagstat] ] + ch_chrom_sizes // channel: [ bed ] + + main: + + ch_versions = Channel.empty() + + // + // Create bedGraph coverage track + // + BEDTOOLS_GENOMECOV ( + ch_bam_flagstat + ) + ch_versions = ch_versions.mix(BEDTOOLS_GENOMECOV.out.versions.first()) + + // + // Create bigWig coverage tracks + // + UCSC_BEDGRAPHTOBIGWIG ( + BEDTOOLS_GENOMECOV.out.bedgraph, + ch_chrom_sizes + ) + ch_versions = ch_versions.mix(UCSC_BEDGRAPHTOBIGWIG.out.versions.first()) + + emit: + bedgraph = BEDTOOLS_GENOMECOV.out.bedgraph // channel: [ val(meta), [ bedgraph ] ] + scale_factor = BEDTOOLS_GENOMECOV.out.scale_factor // channel: [ val(meta), [ txt ] ] + + bigwig = UCSC_BEDGRAPHTOBIGWIG.out.bigwig // channel: [ val(meta), [ bigwig ] ] + + versions = ch_versions // channel: [ versions.yml ] +} From dbbfc239e82129ea13dead9ca5267eda3ecab9eb Mon Sep 17 00:00:00 2001 From: Bjorn Langer Date: Fri, 5 Jul 2024 10:19:54 +0200 Subject: [PATCH 169/201] actually use subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc from nf-core/atacseq --- .../bam_bedgraph_bigwig_bedtools_ucsc.nf | 2 +- workflows/chipseq.nf | 38 ++++++++----------- 2 files changed, 17 insertions(+), 23 deletions(-) diff --git a/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf b/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf index 0947571c..5796f407 100644 --- a/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf +++ b/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf @@ -10,7 +10,7 @@ workflow BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC { take: ch_bam_flagstat // channel: [ val(meta), [bam], [flagstat] ] ch_chrom_sizes // channel: [ bed ] - + main: ch_versions = Channel.empty() diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 8f4a9e63..abdb57d7 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -7,7 +7,6 @@ // // MODULE: Loaded from modules/local/ // -include { BEDTOOLS_GENOMECOV } from '../modules/local/bedtools_genomecov' include { FRIP_SCORE } from '../modules/local/frip_score' include { PLOT_MACS2_QC } from '../modules/local/plot_macs2_qc' include { PLOT_HOMER_ANNOTATEPEAKS } from '../modules/local/plot_homer_annotatepeaks' @@ -22,13 +21,15 @@ include { MULTIQC_CUSTOM_PEAKS } from '../modules/local/multiqc_c // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { paramsSummaryMap } from 'plugin/nf-validation' -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_chipseq_pipeline' -include { INPUT_CHECK } from '../subworkflows/local/input_check' -include { ALIGN_STAR } from '../subworkflows/local/align_star' -include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_chipseq_pipeline' +include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { ALIGN_STAR } from '../subworkflows/local/align_star' +include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' +include { BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC } from '../subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc' +include { BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER } from '../subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -358,21 +359,14 @@ workflow CHIPSEQ { } // - // MODULE: BedGraph coverage tracks + // SUBWORKFLOW: Normalised bigWig coverage tracks // - BEDTOOLS_GENOMECOV ( - BAM_FILTER_BAMTOOLS.out.bam.join(BAM_FILTER_BAMTOOLS.out.flagstat, by: [0]) + BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC ( + BAM_FILTER_BAMTOOLS.out.bam.join(BAM_FILTER_BAMTOOLS.out.flagstat, by: [0]), + PREPARE_GENOME.out.chrom_sizes ) - ch_versions = ch_versions.mix(BEDTOOLS_GENOMECOV.out.versions.first()) + ch_versions = ch_versions.mix(BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC.out.versions) - // - // MODULE: BigWig coverage tracks - // - UCSC_BEDGRAPHTOBIGWIG ( - BEDTOOLS_GENOMECOV.out.bedgraph, - ch_chrom_sizes - ) - ch_versions = ch_versions.mix(UCSC_BEDGRAPHTOBIGWIG.out.versions.first()) ch_deeptoolsplotprofile_multiqc = Channel.empty() if (!params.skip_plot_profile) { @@ -380,7 +374,7 @@ workflow CHIPSEQ { // MODULE: deepTools matrix generation for plotting // DEEPTOOLS_COMPUTEMATRIX ( - UCSC_BEDGRAPHTOBIGWIG.out.bigwig, + BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC.out.bigwig, ch_gene_bed ) ch_versions = ch_versions.mix(DEEPTOOLS_COMPUTEMATRIX.out.versions.first()) @@ -678,7 +672,7 @@ workflow CHIPSEQ { params.aligner, params.narrow_peak ? 'narrow_peak' : 'broad_peak', ch_fasta, - UCSC_BEDGRAPHTOBIGWIG.out.bigwig.collect{it[1]}.ifEmpty([]), + BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC.out.bigwig.collect{it[1]}.ifEmpty([]), ch_macs2_peaks.collect{it[1]}.ifEmpty([]), ch_macs2_consensus_bed_lib.collect{it[1]}.ifEmpty([]), ch_macs2_consensus_txt_lib.collect{it[1]}.ifEmpty([]) From d314ec33aa3b7a97650568461a2ca546367cb622 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Bj=C3=B6rn=20Langer?= <61791748+bjlang@users.noreply.github.com> Date: Fri, 5 Jul 2024 10:26:17 +0200 Subject: [PATCH 170/201] clean imports --- workflows/chipseq.nf | 2 -- 1 file changed, 2 deletions(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index abdb57d7..d1cb3b66 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -29,7 +29,6 @@ include { INPUT_CHECK } from '../subworkflows/local/i include { ALIGN_STAR } from '../subworkflows/local/align_star' include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' include { BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC } from '../subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc' -include { BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER } from '../subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -45,7 +44,6 @@ include { PICARD_MERGESAMFILES } from '../modules/nf-core/picard/merges include { PICARD_COLLECTMULTIPLEMETRICS } from '../modules/nf-core/picard/collectmultiplemetrics/main' include { PRESEQ_LCEXTRAP } from '../modules/nf-core/preseq/lcextrap/main' include { PHANTOMPEAKQUALTOOLS } from '../modules/nf-core/phantompeakqualtools/main' -include { UCSC_BEDGRAPHTOBIGWIG } from '../modules/nf-core/ucsc/bedgraphtobigwig/main' include { DEEPTOOLS_COMPUTEMATRIX } from '../modules/nf-core/deeptools/computematrix/main' include { DEEPTOOLS_PLOTPROFILE } from '../modules/nf-core/deeptools/plotprofile/main' include { DEEPTOOLS_PLOTHEATMAP } from '../modules/nf-core/deeptools/plotheatmap/main' From 8aaccc2a9102bdec64771c1fbf1a48446c12df5a Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Bj=C3=B6rn=20Langer?= <61791748+bjlang@users.noreply.github.com> Date: Fri, 5 Jul 2024 10:30:22 +0200 Subject: [PATCH 171/201] Update chipseq.nf --- workflows/chipseq.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index d1cb3b66..c855693b 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -361,7 +361,7 @@ workflow CHIPSEQ { // BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC ( BAM_FILTER_BAMTOOLS.out.bam.join(BAM_FILTER_BAMTOOLS.out.flagstat, by: [0]), - PREPARE_GENOME.out.chrom_sizes + ch_chrom_sizes ) ch_versions = ch_versions.mix(BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC.out.versions) From 53c152c76341dab3cc9e9d64ec00d6827e4a1ab4 Mon Sep 17 00:00:00 2001 From: =?UTF-8?q?Bj=C3=B6rn=20Langer?= <61791748+bjlang@users.noreply.github.com> Date: Fri, 5 Jul 2024 10:40:56 +0200 Subject: [PATCH 172/201] Update subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf Co-authored-by: Jose Espinosa-Carrasco --- subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf | 1 - 1 file changed, 1 deletion(-) diff --git a/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf b/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf index 5796f407..271c3aa3 100644 --- a/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf +++ b/subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc.nf @@ -1,4 +1,3 @@ - // // Convert BAM to normalised bigWig via bedGraph using BEDTools and UCSC // From a7a9f83514695336a1230c2d18e1709099766673 Mon Sep 17 00:00:00 2001 From: Bjorn Langer Date: Fri, 5 Jul 2024 11:36:06 +0200 Subject: [PATCH 173/201] use subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer from nf-core/atacsec --- .../bam_peaks_call_qc_annotate_macs2_homer.nf | 155 ++++++++++++++++++ workflows/chipseq.nf | 118 ++----------- 2 files changed, 173 insertions(+), 100 deletions(-) create mode 100644 subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf diff --git a/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf b/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf new file mode 100644 index 00000000..4c2a8710 --- /dev/null +++ b/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf @@ -0,0 +1,155 @@ +// +// Call peaks with MACS2, annotate with HOMER and perform downstream QC +// + +include { MACS2_CALLPEAK } from '../../modules/nf-core/macs2/callpeak/main' +include { HOMER_ANNOTATEPEAKS } from '../../modules/nf-core/homer/annotatepeaks/main' + +include { FRIP_SCORE } from '../../modules/local/frip_score' +include { MULTIQC_CUSTOM_PEAKS } from '../../modules/local/multiqc_custom_peaks' +include { PLOT_MACS2_QC } from '../../modules/local/plot_macs2_qc' +include { PLOT_HOMER_ANNOTATEPEAKS } from '../../modules/local/plot_homer_annotatepeaks' + +workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { + take: + ch_bam // channel: [ val(meta), [ ip_bam ], [ control_bam ] ] + ch_fasta // channel: [ fasta ] + ch_gtf // channel: [ gtf ] + macs_gsize // integer: value for --macs_gsize parameter + annotate_peaks_suffix // string: suffix for input HOMER annotate peaks files to be trimmed off + ch_peak_count_header_multiqc // channel: [ header_file ] + ch_frip_score_multiqc // channel: [ header_file ] + ch_peak_annotation_header_multiqc // channel: [ header_file ] + is_narrow_peak // boolean: true/false + skip_peak_annotation // boolean: true/false + skip_peak_qc // boolean: true/false + + main: + + ch_versions = Channel.empty() + + // + // Call peaks with MACS2 + // + MACS2_CALLPEAK ( + ch_bam, + macs_gsize + ) + ch_versions = ch_versions.mix(MACS2_CALLPEAK.out.versions.first()) + + // + // Filter out samples with 0 MACS2 peaks called + // + MACS2_CALLPEAK + .out + .peak + .filter { + meta, peaks -> + peaks.size() > 0 + } + .set { ch_macs2_peaks } + + // Create channels: [ meta, ip_bam, peaks ] + ch_bam + .join(ch_macs2_peaks, by: [0]) + .map { + meta, ip_bam, control_bam, peaks -> + [ meta, ip_bam, peaks ] + } + .set { ch_bam_peaks } + + // + // Calculate FRiP score + // + FRIP_SCORE ( + ch_bam_peaks + ) + ch_versions = ch_versions.mix(FRIP_SCORE.out.versions.first()) + + // Create channels: [ meta, peaks, frip ] + ch_bam_peaks + .join(FRIP_SCORE.out.txt, by: [0]) + .map { + meta, ip_bam, peaks, frip -> + [ meta, peaks, frip ] + } + .set { ch_bam_peak_frip } + + // + // FRiP score custom content for MultiQC + // + MULTIQC_CUSTOM_PEAKS ( + ch_bam_peak_frip, + ch_peak_count_header_multiqc, + ch_frip_score_multiqc + ) + ch_versions = ch_versions.mix(MULTIQC_CUSTOM_PEAKS.out.versions.first()) + + ch_homer_annotatepeaks = Channel.empty() + ch_plot_macs2_qc_txt = Channel.empty() + ch_plot_macs2_qc_pdf = Channel.empty() + ch_plot_homer_annotatepeaks_txt = Channel.empty() + ch_plot_homer_annotatepeaks_pdf = Channel.empty() + ch_plot_homer_annotatepeaks_tsv = Channel.empty() + if (!skip_peak_annotation) { + // + // Annotate peaks with HOMER + // + HOMER_ANNOTATEPEAKS ( + ch_macs2_peaks, + ch_fasta, + ch_gtf + ) + ch_homer_annotatepeaks = HOMER_ANNOTATEPEAKS.out.txt + ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS.out.versions.first()) + + if (!skip_peak_qc) { + // + // MACS2 QC plots with R + // + PLOT_MACS2_QC ( + ch_macs2_peaks.collect{it[1]}, + is_narrow_peak + ) + ch_plot_macs2_qc_txt = PLOT_MACS2_QC.out.txt + ch_plot_macs2_qc_pdf = PLOT_MACS2_QC.out.pdf + ch_versions = ch_versions.mix(PLOT_MACS2_QC.out.versions) + + // + // Peak annotation QC plots with R + // + PLOT_HOMER_ANNOTATEPEAKS ( + HOMER_ANNOTATEPEAKS.out.txt.collect{it[1]}, + ch_peak_annotation_header_multiqc, + annotate_peaks_suffix + ) + ch_plot_homer_annotatepeaks_txt = PLOT_HOMER_ANNOTATEPEAKS.out.txt + ch_plot_homer_annotatepeaks_pdf = PLOT_HOMER_ANNOTATEPEAKS.out.pdf + ch_plot_homer_annotatepeaks_tsv = PLOT_HOMER_ANNOTATEPEAKS.out.tsv + ch_versions = ch_versions.mix(PLOT_HOMER_ANNOTATEPEAKS.out.versions) + } + } + + emit: + peaks = ch_macs2_peaks // channel: [ val(meta), [ peaks ] ] + xls = MACS2_CALLPEAK.out.xls // channel: [ val(meta), [ xls ] ] + gapped_peaks = MACS2_CALLPEAK.out.gapped // channel: [ val(meta), [ gapped_peak ] ] + bed = MACS2_CALLPEAK.out.bed // channel: [ val(meta), [ bed ] ] + bedgraph = MACS2_CALLPEAK.out.bdg // channel: [ val(meta), [ bedgraph ] ] + + frip_txt = FRIP_SCORE.out.txt // channel: [ val(meta), [ txt ] ] + + frip_multiqc = MULTIQC_CUSTOM_PEAKS.out.frip // channel: [ val(meta), [ frip ] ] + peak_count_multiqc = MULTIQC_CUSTOM_PEAKS.out.count // channel: [ val(meta), [ counts ] ] + + homer_annotatepeaks = ch_homer_annotatepeaks // channel: [ val(meta), [ txt ] ] + + plot_macs2_qc_txt = ch_plot_macs2_qc_txt // channel: [ txt ] + plot_macs2_qc_pdf = ch_plot_macs2_qc_pdf // channel: [ pdf ] + + plot_homer_annotatepeaks_txt = ch_plot_homer_annotatepeaks_txt // channel: [ txt ] + plot_homer_annotatepeaks_pdf = ch_plot_homer_annotatepeaks_pdf // channel: [ pdf ] + plot_homer_annotatepeaks_tsv = ch_plot_homer_annotatepeaks_tsv // channel: [ tsv ] + + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index c855693b..f7a19072 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -7,16 +7,12 @@ // // MODULE: Loaded from modules/local/ // -include { FRIP_SCORE } from '../modules/local/frip_score' -include { PLOT_MACS2_QC } from '../modules/local/plot_macs2_qc' -include { PLOT_HOMER_ANNOTATEPEAKS } from '../modules/local/plot_homer_annotatepeaks' include { MACS2_CONSENSUS } from '../modules/local/macs2_consensus' include { ANNOTATE_BOOLEAN_PEAKS } from '../modules/local/annotate_boolean_peaks' include { DESEQ2_QC } from '../modules/local/deseq2_qc' include { IGV } from '../modules/local/igv' include { MULTIQC } from '../modules/local/multiqc' include { MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS } from '../modules/local/multiqc_custom_phantompeakqualtools' -include { MULTIQC_CUSTOM_PEAKS } from '../modules/local/multiqc_custom_peaks' // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules @@ -29,6 +25,7 @@ include { INPUT_CHECK } from '../subworkflows/local/i include { ALIGN_STAR } from '../subworkflows/local/align_star' include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' include { BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC } from '../subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc' +include { BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER } from '../subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -49,10 +46,8 @@ include { DEEPTOOLS_PLOTPROFILE } from '../modules/nf-core/deeptools/plo include { DEEPTOOLS_PLOTHEATMAP } from '../modules/nf-core/deeptools/plotheatmap/main' include { DEEPTOOLS_PLOTFINGERPRINT } from '../modules/nf-core/deeptools/plotfingerprint/main' include { KHMER_UNIQUEKMERS } from '../modules/nf-core/khmer/uniquekmers/main' -include { MACS2_CALLPEAK } from '../modules/nf-core/macs2/callpeak/main' include { SUBREAD_FEATURECOUNTS } from '../modules/nf-core/subread/featurecounts/main' -include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_MACS2 } from '../modules/nf-core/homer/annotatepeaks/main' include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../modules/nf-core/homer/annotatepeaks/main' // @@ -437,9 +432,6 @@ workflow CHIPSEQ { // // TODO move to prepare genome ch_macs_gsize = Channel.empty() - ch_custompeaks_frip_multiqc = Channel.empty() - ch_custompeaks_count_multiqc = Channel.empty() - ch_plothomerannotatepeaks_multiqc = Channel.empty() ch_subreadfeaturecounts_multiqc = Channel.empty() ch_macs_gsize = params.macs_gsize if (!params.macs_gsize) { @@ -459,96 +451,22 @@ workflow CHIPSEQ { .set { ch_ip_control_bam } // - // MODULE: Call peaks with MACS2 + // SUBWORKFLOW: Call peaks with MACS2, annotate with HOMER and perform downstream QC // - MACS2_CALLPEAK ( + BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER ( ch_ip_control_bam, - ch_macs_gsize - ) - ch_versions = ch_versions.mix(MACS2_CALLPEAK.out.versions.first()) - - // - // Filter out samples with 0 MACS2 peaks called - // - MACS2_CALLPEAK - .out - .peak - .filter { - meta, peaks -> - peaks.size() > 0 - } - .set { ch_macs2_peaks } - - // Create channels: [ meta, ip_bam, peaks ] - ch_ip_control_bam - .join(ch_macs2_peaks, by: [0]) - .map { - it -> - [ it[0], it[1], it[3] ] - } - .set { ch_ip_bam_peaks } - - // - // MODULE: Calculate FRiP score - // - FRIP_SCORE ( - ch_ip_bam_peaks - ) - ch_versions = ch_versions.mix(FRIP_SCORE.out.versions.first()) - - // Create channels: [ meta, peaks, frip ] - ch_ip_bam_peaks - .join(FRIP_SCORE.out.txt, by: [0]) - .map { - it -> - [ it[0], it[2], it[3] ] - } - .set { ch_ip_peaks_frip } - - // - // MODULE: FRiP score custom content for MultiQC - // - MULTIQC_CUSTOM_PEAKS ( - ch_ip_peaks_frip, + ch_fasta, + ch_gtf, + ch_macs_gsize, + "_peaks.annotatePeaks.txt", ch_peak_count_header, - ch_frip_score_header + ch_frip_score_header, + ch_peak_annotation_header, + params.narrow_peak, + params.skip_peak_annotation, + params.skip_peak_qc ) - ch_custompeaks_frip_multiqc = MULTIQC_CUSTOM_PEAKS.out.frip - ch_custompeaks_count_multiqc = MULTIQC_CUSTOM_PEAKS.out.count - - if (!params.skip_peak_annotation) { - // - // MODULE: Annotate peaks with MACS2 - // - HOMER_ANNOTATEPEAKS_MACS2 ( - ch_macs2_peaks, - ch_fasta, - ch_gtf - ) - ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS_MACS2.out.versions.first()) - - if (!params.skip_peak_qc) { - // - // MODULE: MACS2 QC plots with R - // - PLOT_MACS2_QC ( - ch_macs2_peaks.collect{it[1]}, - params.narrow_peak - ) - ch_versions = ch_versions.mix(PLOT_MACS2_QC.out.versions) - - // - // MODULE: Peak annotation QC plots with R - // - PLOT_HOMER_ANNOTATEPEAKS ( - HOMER_ANNOTATEPEAKS_MACS2.out.txt.collect{it[1]}, - ch_peak_annotation_header, - "_peaks.annotatePeaks.txt" - ) - ch_plothomerannotatepeaks_multiqc = PLOT_HOMER_ANNOTATEPEAKS.out.tsv - ch_versions = ch_versions.mix(PLOT_HOMER_ANNOTATEPEAKS.out.versions) - } - } + ch_versions = ch_versions.mix(MERGED_LIBRARY_CALL_ANNOTATE_PEAKS.out.versions) // // Consensus peaks analysis @@ -560,7 +478,7 @@ workflow CHIPSEQ { if (!params.skip_consensus_peaks) { // Create channels: [ meta , [ peaks ] ] // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] - ch_macs2_peaks + BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peaks .map { meta, peak -> [ meta.antibody, meta.id.split('_')[0..-2].join('_'), peak ] @@ -671,7 +589,7 @@ workflow CHIPSEQ { params.narrow_peak ? 'narrow_peak' : 'broad_peak', ch_fasta, BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC.out.bigwig.collect{it[1]}.ifEmpty([]), - ch_macs2_peaks.collect{it[1]}.ifEmpty([]), + BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peaks.collect{it[1]}.ifEmpty([]), ch_macs2_consensus_bed_lib.collect{it[1]}.ifEmpty([]), ch_macs2_consensus_txt_lib.collect{it[1]}.ifEmpty([]) ) @@ -731,9 +649,9 @@ workflow CHIPSEQ { ch_multiqc_phantompeakqualtools_rsc_multiqc.collect{it[1]}.ifEmpty([]), ch_multiqc_phantompeakqualtools_correlation_multiqc.collect{it[1]}.ifEmpty([]), - ch_custompeaks_frip_multiqc.collect{it[1]}.ifEmpty([]), - ch_custompeaks_count_multiqc.collect{it[1]}.ifEmpty([]), - ch_plothomerannotatepeaks_multiqc.collect().ifEmpty([]), + BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.frip_multiqc.collect{it[1]}.ifEmpty([]), + BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peak_count_multiqc.collect{it[1]}.ifEmpty([]), + BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.plot_homer_annotatepeaks_tsv.collect().ifEmpty([]), ch_subreadfeaturecounts_multiqc.collect{it[1]}.ifEmpty([]), ch_deseq2_pca_multiqc.collect().ifEmpty([]), From 561b73b8eee95b2d8e872e66a5b2aad1883de63b Mon Sep 17 00:00:00 2001 From: bjlang <> Date: Fri, 5 Jul 2024 13:22:51 +0200 Subject: [PATCH 174/201] Update modules.config and other minor corrections --- conf/modules.config | 50 +++++++++---------- .../bam_peaks_call_qc_annotate_macs2_homer.nf | 8 +-- workflows/chipseq.nf | 2 +- 3 files changed, 30 insertions(+), 30 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index 59af9e66..1fc095e3 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -369,14 +369,21 @@ process { ] } - withName: 'BAM_REMOVE_ORPHANS' { - ext.args = '--only_fr_pairs' - ext.prefix = { "${meta.id}.mLb.clN" } + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:SAMTOOLS_INDEX' { + ext.prefix = { "${meta.id}.mLb.clN.sorted" } publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library" }, mode: params.publish_dir_mode, - pattern: '*.bam', - enabled: params.save_align_intermeds + pattern: '*.{bai,csi}' + ] + } + + withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:BAM_STATS_SAMTOOLS:.*' { + ext.prefix = { "${meta.id}.mLb.clN.sorted.bam" } + publishDir = [ + path: { "${params.outdir}/${params.aligner}/merged_library/samtools_stats" }, + mode: params.publish_dir_mode, + pattern: "*.{stats,flagstat,idxstats}" ] } @@ -391,21 +398,14 @@ process { ] } - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:SAMTOOLS_INDEX' { - ext.prefix = { "${meta.id}.mLb.clN.sorted" } + withName: 'BAM_REMOVE_ORPHANS' { + ext.args = '--only_fr_pairs' + ext.prefix = { "${meta.id}.mLb.clN" } publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library" }, mode: params.publish_dir_mode, - pattern: '*.{bai,csi}' - ] - } - - withName: 'NFCORE_CHIPSEQ:CHIPSEQ:BAM_FILTER_BAMTOOLS:BAM_STATS_SAMTOOLS:.*' { - ext.prefix = { "${meta.id}.mLb.clN.sorted.bam" } - publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/samtools_stats" }, - mode: params.publish_dir_mode, - pattern: "*.{stats,flagstat,idxstats}" + pattern: '*.bam', + enabled: params.save_align_intermeds ] } @@ -427,7 +427,7 @@ process { ] } - withName: 'BEDTOOLS_GENOMECOV' { + withName: '.*:BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC:BEDTOOLS_GENOMECOV' { ext.args = { (meta.single_end && params.fragment_size > 0) ? "-fs ${params.fragment_size}" : '' } ext.prefix = { "${meta.id}.mLB.clN" } publishDir = [ @@ -444,7 +444,7 @@ process { ] } - withName: 'UCSC_BEDGRAPHTOBIGWIG' { + withName: '.*:BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC:UCSC_BEDGRAPHTOBIGWIG' { ext.prefix = { "${meta.id}.mLb.clN" } publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/bigwig" }, @@ -540,7 +540,7 @@ if (!params.skip_plot_fingerprint) { } process { - withName: 'MACS2_CALLPEAK' { + withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:MACS2_CALLPEAK' { ext.args = [ '--keep-dup all', params.narrow_peak ? '' : "--broad --broad-cutoff ${params.broad_cutoff}", @@ -559,7 +559,7 @@ process { ] } - withName: 'FRIP_SCORE' { + withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:FRIP_SCORE' { ext.args = '-bed -c -f 0.20' publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, @@ -567,7 +567,7 @@ process { ] } - withName: 'MULTIQC_CUSTOM_PEAKS' { + withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:MULTIQC_CUSTOM_PEAKS' { publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, @@ -578,7 +578,7 @@ process { if (!params.skip_peak_annotation) { process { - withName: 'HOMER_ANNOTATEPEAKS_MACS2' { + withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:HOMER_ANNOTATEPEAKS' { ext.args = '-gid' ext.prefix = { "${meta.id}_peaks" } publishDir = [ @@ -591,7 +591,7 @@ if (!params.skip_peak_annotation) { if (!params.skip_peak_qc) { process { - withName: 'PLOT_MACS2_QC' { + withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:PLOT_MACS2_QC' { ext.args = '-o ./ -p macs2_peak' publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, @@ -600,7 +600,7 @@ if (!params.skip_peak_annotation) { ] } - withName: 'PLOT_HOMER_ANNOTATEPEAKS' { + withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:PLOT_HOMER_ANNOTATEPEAKS' { ext.args = '-o ./' ext.prefix = 'macs2_annotatePeaks' publishDir = [ diff --git a/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf b/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf index 4c2a8710..5b7b9b85 100644 --- a/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf +++ b/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf @@ -23,7 +23,7 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { is_narrow_peak // boolean: true/false skip_peak_annotation // boolean: true/false skip_peak_qc // boolean: true/false - + main: ch_versions = Channel.empty() @@ -43,7 +43,7 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { MACS2_CALLPEAK .out .peak - .filter { + .filter { meta, peaks -> peaks.size() > 0 } @@ -102,7 +102,7 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { ) ch_homer_annotatepeaks = HOMER_ANNOTATEPEAKS.out.txt ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS.out.versions.first()) - + if (!skip_peak_qc) { // // MACS2 QC plots with R @@ -138,7 +138,7 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { bedgraph = MACS2_CALLPEAK.out.bdg // channel: [ val(meta), [ bedgraph ] ] frip_txt = FRIP_SCORE.out.txt // channel: [ val(meta), [ txt ] ] - + frip_multiqc = MULTIQC_CUSTOM_PEAKS.out.frip // channel: [ val(meta), [ frip ] ] peak_count_multiqc = MULTIQC_CUSTOM_PEAKS.out.count // channel: [ val(meta), [ counts ] ] diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index f7a19072..c7ba425d 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -466,7 +466,7 @@ workflow CHIPSEQ { params.skip_peak_annotation, params.skip_peak_qc ) - ch_versions = ch_versions.mix(MERGED_LIBRARY_CALL_ANNOTATE_PEAKS.out.versions) + ch_versions = ch_versions.mix(BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.versions) // // Consensus peaks analysis From bb76db77da3c7c676741e28c7e9a30ee64461144 Mon Sep 17 00:00:00 2001 From: bjlang <> Date: Mon, 8 Jul 2024 14:11:12 +0200 Subject: [PATCH 175/201] create subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer similar to nf-core/atacseq --- conf/modules.config | 10 +- ...antify_qc_bedtools_featurecounts_deseq2.nf | 156 ++++++++++++++++++ workflows/chipseq.nf | 127 +++----------- 3 files changed, 187 insertions(+), 106 deletions(-) create mode 100644 subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf diff --git a/conf/modules.config b/conf/modules.config index 1fc095e3..a18fa85c 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -615,7 +615,7 @@ if (!params.skip_peak_annotation) { if (!params.skip_consensus_peaks) { process { - withName: 'MACS2_CONSENSUS' { + withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:MACS2_CONSENSUS' { ext.when = { meta.multiple_groups || meta.replicates_exist } ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ @@ -625,7 +625,7 @@ if (!params.skip_consensus_peaks) { ] } - withName: 'SUBREAD_FEATURECOUNTS' { + withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:SUBREAD_FEATURECOUNTS' { ext.args = '-F SAF -O --fracOverlap 0.2' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ @@ -638,7 +638,7 @@ if (!params.skip_consensus_peaks) { if (!params.skip_peak_annotation) { process { - withName: 'HOMER_ANNOTATEPEAKS_CONSENSUS' { + withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:HOMER_ANNOTATEPEAKS' { ext.args = '-gid' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ @@ -648,7 +648,7 @@ if (!params.skip_consensus_peaks) { ] } - withName: 'ANNOTATE_BOOLEAN_PEAKS' { + withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:ANNOTATE_BOOLEAN_PEAKS' { ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, @@ -661,7 +661,7 @@ if (!params.skip_consensus_peaks) { if (!params.skip_deseq2_qc) { process { - withName: DESEQ2_QC { + withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:DESEQ2_QC' { ext.when = { meta.multiple_groups && meta.replicates_exist } ext.args = [ '--id_col 1', diff --git a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf new file mode 100644 index 00000000..98e4bc80 --- /dev/null +++ b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf @@ -0,0 +1,156 @@ +// +// Call consensus peaks with BEDTools and custom scripts, annotate with HOMER, quantify with featureCounts and QC with DESeq2 +// + +include { HOMER_ANNOTATEPEAKS } from '../../modules/nf-core/homer/annotatepeaks/main' +include { SUBREAD_FEATURECOUNTS } from '../../modules/nf-core/subread/featurecounts/main' + +include { MACS2_CONSENSUS } from '../../modules/local/macs2_consensus' +include { DESEQ2_QC } from '../../modules/local/deseq2_qc' + +workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { + take: + ch_peaks // channel: [ val(meta), [ peaks ] ] + ch_bams // channel: [ val(meta), [ ip_bams ] ] + ch_fasta // channel: [ fasta ] + ch_gtf // channel: [ gtf ] + ch_deseq2_pca_header_multiqc // channel: [ header_file ] + ch_deseq2_clustering_header_multiqc // channel: [ header_file ] + is_narrow_peak // boolean: true/false + skip_peak_annotation // boolean: true/false + skip_deseq2_qc // boolean: true/false + + main: + + ch_versions = Channel.empty() + + // Create channels: [ meta , [ peaks ] ] + // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] + ch_peaks + .map { + meta, peak -> + [ meta.antibody, meta.id.split('_')[0..-2].join('_'), peak ] + } + .groupTuple() + .map { + antibody, groups, peaks -> + [ + antibody, + groups.groupBy().collectEntries { [(it.key) : it.value.size()] }, + peaks + ] + } + .map { + antibody, groups, peaks -> + def meta_new = [:] + meta_new.id = antibody + meta_new.multiple_groups = groups.size() > 1 + meta_new.replicates_exist = groups.max { groups.value }.value > 1 + [ meta_new, peaks ] + } + .set { ch_antibody_peaks } + + // + // Generate consensus peaks across samples + // + MACS2_CONSENSUS ( + ch_antibody_peaks, + is_narrow_peak + ) + ch_versions = ch_versions.mix(MACS2_CONSENSUS.out.versions) + + // + // Annotate consensus peaks + // + if (!skip_peak_annotation) { + HOMER_ANNOTATEPEAKS ( + MACS2_CONSENSUS.out.bed, + ch_fasta, + ch_gtf + ) + + // + // MODULE: Add boolean fields to annotated consensus peaks to aid filtering + // + ANNOTATE_BOOLEAN_PEAKS ( + MACS2_CONSENSUS.out.boolean_txt.join(HOMER_ANNOTATEPEAKS.out.txt, by: [0]), + ) + ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS.out.versions) + } + + // Create channels: [ meta, [ ip_bams ], saf ] + MACS2_CONSENSUS + .out + .saf + .map { + meta, saf -> + [ meta.id, meta, saf ] + } + .join(ch_bams) + .map { + antibody, meta, saf, bams -> + [ meta, bams.flatten().sort(), saf ] + } + .set { ch_bam_saf } + + // + // Quantify peaks across samples with featureCounts + // + SUBREAD_FEATURECOUNTS ( + ch_bam_saf + ) + ch_versions = ch_versions.mix(SUBREAD_FEATURECOUNTS.out.versions) + + // + // Generate QC plots with DESeq2 + // + ch_deseq2_qc_pdf = Channel.empty() + ch_deseq2_qc_rdata = Channel.empty() + ch_deseq2_qc_rds = Channel.empty() + ch_deseq2_qc_pca_txt = Channel.empty() + ch_deseq2_qc_pca_multiqc = Channel.empty() + ch_deseq2_qc_dists_txt = Channel.empty() + ch_deseq2_qc_dists_multiqc = Channel.empty() + ch_deseq2_qc_log = Channel.empty() + ch_deseq2_qc_size_factors = Channel.empty() + if (!skip_deseq2_qc) { + DESEQ2_QC ( + SUBREAD_FEATURECOUNTS.out.counts, + ch_deseq2_pca_header_multiqc, + ch_deseq2_clustering_header_multiqc + ) + ch_deseq2_qc_pdf = DESEQ2_QC.out.pdf + ch_deseq2_qc_rdata = DESEQ2_QC.out.rdata + ch_deseq2_qc_rds = DESEQ2_QC.out.rds + ch_deseq2_qc_pca_txt = DESEQ2_QC.out.pca_txt + ch_deseq2_qc_pca_multiqc = DESEQ2_QC.out.pca_multiqc + ch_deseq2_qc_dists_txt = DESEQ2_QC.out.dists_txt + ch_deseq2_qc_dists_multiqc = DESEQ2_QC.out.dists_multiqc + ch_deseq2_qc_log = DESEQ2_QC.out.log + ch_deseq2_qc_size_factors = DESEQ2_QC.out.size_factors + ch_versions = ch_versions.mix(DESEQ2_QC.out.versions) + } + + emit: + consensus_bed = MACS2_CONSENSUS.out.bed // channel: [ bed ] + consensus_saf = MACS2_CONSENSUS.out.saf // channel: [ saf ] + consensus_pdf = MACS2_CONSENSUS.out.pdf // channel: [ pdf ] + consensus_txt = MACS2_CONSENSUS.out.txt // channel: [ pdf ] + consensus_boolean_txt = MACS2_CONSENSUS.out.boolean_txt // channel: [ txt ] + consensus_intersect_txt = MACS2_CONSENSUS.out.intersect_txt // channel: [ txt ] + + featurecounts_txt = SUBREAD_FEATURECOUNTS.out.counts // channel: [ txt ] + featurecounts_summary = SUBREAD_FEATURECOUNTS.out.summary // channel: [ txt ] + + deseq2_qc_pdf = ch_deseq2_qc_pdf // channel: [ pdf ] + deseq2_qc_rdata = ch_deseq2_qc_rdata // channel: [ rdata ] + deseq2_qc_rds = ch_deseq2_qc_rds // channel: [ rds ] + deseq2_qc_pca_txt = ch_deseq2_qc_pca_txt // channel: [ txt ] + deseq2_qc_pca_multiqc = ch_deseq2_qc_pca_multiqc // channel: [ txt ] + deseq2_qc_dists_txt = ch_deseq2_qc_dists_txt // channel: [ txt ] + deseq2_qc_dists_multiqc = ch_deseq2_qc_dists_multiqc // channel: [ txt ] + deseq2_qc_log = ch_deseq2_qc_log // channel: [ txt ] + deseq2_qc_size_factors = ch_deseq2_qc_size_factors // channel: [ txt ] + + versions = ch_versions // channel: [ versions.yml ] +} diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index c7ba425d..3b8ab88a 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -17,15 +17,16 @@ include { MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS } from '../modules/local/multiqc_c // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { paramsSummaryMap } from 'plugin/nf-validation' -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_chipseq_pipeline' -include { INPUT_CHECK } from '../subworkflows/local/input_check' -include { ALIGN_STAR } from '../subworkflows/local/align_star' -include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' -include { BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC } from '../subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc' -include { BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER } from '../subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_chipseq_pipeline' +include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { ALIGN_STAR } from '../subworkflows/local/align_star' +include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' +include { BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC } from '../subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc' +include { BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER } from '../subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf' +include { BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 } from '../subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -476,63 +477,6 @@ workflow CHIPSEQ { ch_deseq2_pca_multiqc = Channel.empty() ch_deseq2_clustering_multiqc = Channel.empty() if (!params.skip_consensus_peaks) { - // Create channels: [ meta , [ peaks ] ] - // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] - BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peaks - .map { - meta, peak -> - [ meta.antibody, meta.id.split('_')[0..-2].join('_'), peak ] - } - .groupTuple() - .map { - antibody, groups, peaks -> - [ - antibody, - groups.groupBy().collectEntries { [(it.key) : it.value.size()] }, - peaks - ] - } - .map { - antibody, groups, peaks -> - def meta_new = [:] - meta_new.id = antibody - meta_new.multiple_groups = groups.size() > 1 - meta_new.replicates_exist = groups.max { groups.value }.value > 1 - [ meta_new, peaks ] - } - .set { ch_antibody_peaks } - - // - // MODULE: Generate consensus peaks across samples - // - MACS2_CONSENSUS ( - ch_antibody_peaks, - params.narrow_peak - ) - ch_macs2_consensus_bed_lib = MACS2_CONSENSUS.out.bed - ch_macs2_consensus_txt_lib = MACS2_CONSENSUS.out.txt - ch_versions = ch_versions.mix(MACS2_CONSENSUS.out.versions) - - if (!params.skip_peak_annotation) { - // - // MODULE: Annotate consensus peaks - // - HOMER_ANNOTATEPEAKS_CONSENSUS ( - MACS2_CONSENSUS.out.bed, - ch_fasta, - ch_gtf - ) - ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS_CONSENSUS.out.versions) - - // - // MODULE: Add boolean fields to annotated consensus peaks to aid filtering - // - ANNOTATE_BOOLEAN_PEAKS ( - MACS2_CONSENSUS.out.boolean_txt.join(HOMER_ANNOTATEPEAKS_CONSENSUS.out.txt, by: [0]), - ) - ch_versions = ch_versions.mix(ANNOTATE_BOOLEAN_PEAKS.out.versions) - } - // Create channels: [ antibody, [ ip_bams ] ] ch_ip_control_bam .map { @@ -542,42 +486,23 @@ workflow CHIPSEQ { .groupTuple() .set { ch_antibody_bams } - // Create channels: [ meta, [ ip_bams ], saf ] - MACS2_CONSENSUS - .out - .saf - .map { - meta, saf -> - [ meta.id, meta, saf ] - } - .join(ch_antibody_bams) - .map { - antibody, meta, saf, bams -> - [ meta, bams.flatten().sort(), saf ] - } - .set { ch_saf_bams } - - // - // MODULE: Quantify peaks across samples with featureCounts - // - SUBREAD_FEATURECOUNTS ( - ch_saf_bams + BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 ( + BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peaks, + ch_antibody_bams, + ch_fasta, + ch_gtf, + ch_deseq2_pca_header, + ch_deseq2_clustering_header, + params.narrow_peak, + params.skip_peak_annotation, + params.skip_deseq2_qc ) - ch_subreadfeaturecounts_multiqc = SUBREAD_FEATURECOUNTS.out.summary - ch_versions = ch_versions.mix(SUBREAD_FEATURECOUNTS.out.versions.first()) - - if (!params.skip_deseq2_qc) { - // - // MODULE: Generate QC plots with DESeq2 - // - DESEQ2_QC ( - SUBREAD_FEATURECOUNTS.out.counts, - ch_deseq2_pca_header, - ch_deseq2_clustering_header - ) - ch_deseq2_pca_multiqc = DESEQ2_QC.out.pca_multiqc - ch_deseq2_clustering_multiqc = DESEQ2_QC.out.dists_multiqc - } + ch_macs2_consensus_bed_lib = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.consensus_bed + ch_macs2_consensus_txt_lib = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.consensus_txt + ch_subreadfeaturecounts_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.featurecounts_summary + ch_deseq2_pca_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.deseq2_qc_pca_multiqc + ch_deseq2_clustering_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.deseq2_qc_dists_multiqc + ch_versions = ch_versions.mix(BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.versions) } // From 132892e796b6de36949fd1e19692d632ee57c7a7 Mon Sep 17 00:00:00 2001 From: bjlang <> Date: Mon, 8 Jul 2024 14:17:49 +0200 Subject: [PATCH 176/201] fix includes --- .../bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf | 1 + workflows/chipseq.nf | 1 - 2 files changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf index 98e4bc80..d4d3860d 100644 --- a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf +++ b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf @@ -6,6 +6,7 @@ include { HOMER_ANNOTATEPEAKS } from '../../modules/nf-core/homer/annotatepea include { SUBREAD_FEATURECOUNTS } from '../../modules/nf-core/subread/featurecounts/main' include { MACS2_CONSENSUS } from '../../modules/local/macs2_consensus' +include { ANNOTATE_BOOLEAN_PEAKS } from '../../modules/local/annotate_boolean_peaks' include { DESEQ2_QC } from '../../modules/local/deseq2_qc' workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 3b8ab88a..6f722b6f 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -8,7 +8,6 @@ // MODULE: Loaded from modules/local/ // include { MACS2_CONSENSUS } from '../modules/local/macs2_consensus' -include { ANNOTATE_BOOLEAN_PEAKS } from '../modules/local/annotate_boolean_peaks' include { DESEQ2_QC } from '../modules/local/deseq2_qc' include { IGV } from '../modules/local/igv' include { MULTIQC } from '../modules/local/multiqc' From f4f8b689d29a947d8498fac389388b288f2375c5 Mon Sep 17 00:00:00 2001 From: bjlang <> Date: Tue, 9 Jul 2024 09:49:35 +0200 Subject: [PATCH 177/201] suggestions from code review --- conf/modules.config | 18 +++++++++--------- ...uantify_qc_bedtools_featurecounts_deseq2.nf | 3 ++- 2 files changed, 11 insertions(+), 10 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index a18fa85c..e374f867 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -540,7 +540,7 @@ if (!params.skip_plot_fingerprint) { } process { - withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:MACS2_CALLPEAK' { + withName: 'MACS2_CALLPEAK' { ext.args = [ '--keep-dup all', params.narrow_peak ? '' : "--broad --broad-cutoff ${params.broad_cutoff}", @@ -559,7 +559,7 @@ process { ] } - withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:FRIP_SCORE' { + withName: 'FRIP_SCORE' { ext.args = '-bed -c -f 0.20' publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, @@ -567,7 +567,7 @@ process { ] } - withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:MULTIQC_CUSTOM_PEAKS' { + withName: 'MULTIQC_CUSTOM_PEAKS' { publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, @@ -591,7 +591,7 @@ if (!params.skip_peak_annotation) { if (!params.skip_peak_qc) { process { - withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:PLOT_MACS2_QC' { + withName: 'PLOT_MACS2_QC' { ext.args = '-o ./ -p macs2_peak' publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, @@ -600,7 +600,7 @@ if (!params.skip_peak_annotation) { ] } - withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:PLOT_HOMER_ANNOTATEPEAKS' { + withName: 'PLOT_HOMER_ANNOTATEPEAKS' { ext.args = '-o ./' ext.prefix = 'macs2_annotatePeaks' publishDir = [ @@ -615,7 +615,7 @@ if (!params.skip_peak_annotation) { if (!params.skip_consensus_peaks) { process { - withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:MACS2_CONSENSUS' { + withName: 'MACS2_CONSENSUS' { ext.when = { meta.multiple_groups || meta.replicates_exist } ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ @@ -625,7 +625,7 @@ if (!params.skip_consensus_peaks) { ] } - withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:SUBREAD_FEATURECOUNTS' { + withName: 'SUBREAD_FEATURECOUNTS' { ext.args = '-F SAF -O --fracOverlap 0.2' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ @@ -648,7 +648,7 @@ if (!params.skip_consensus_peaks) { ] } - withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:ANNOTATE_BOOLEAN_PEAKS' { + withName: 'ANNOTATE_BOOLEAN_PEAKS' { ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, @@ -661,7 +661,7 @@ if (!params.skip_consensus_peaks) { if (!params.skip_deseq2_qc) { process { - withName: '.*:BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2:DESEQ2_QC' { + withName: 'DESEQ2_QC' { ext.when = { meta.multiple_groups && meta.replicates_exist } ext.args = [ '--id_col 1', diff --git a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf index d4d3860d..e3a38432 100644 --- a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf +++ b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf @@ -69,6 +69,7 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { ch_fasta, ch_gtf ) + ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS.out.versions) // // MODULE: Add boolean fields to annotated consensus peaks to aid filtering @@ -76,7 +77,7 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { ANNOTATE_BOOLEAN_PEAKS ( MACS2_CONSENSUS.out.boolean_txt.join(HOMER_ANNOTATEPEAKS.out.txt, by: [0]), ) - ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS.out.versions) + ch_versions = ch_versions.mix(ANNOTATE_BOOLEAN_PEAKS.out.versions) } // Create channels: [ meta, [ ip_bams ], saf ] From 1bd1b1583a00b0bcc34d3959d02d2a03a0282ea6 Mon Sep 17 00:00:00 2001 From: bjlang <> Date: Tue, 9 Jul 2024 17:41:27 +0200 Subject: [PATCH 178/201] Clean up and removal of double slashes --- README.md | 2 +- conf/modules.config | 29 +++++++++---------- ...antify_qc_bedtools_featurecounts_deseq2.nf | 2 +- workflows/chipseq.nf | 11 ++----- 4 files changed, 18 insertions(+), 26 deletions(-) diff --git a/README.md b/README.md index 2dcb70e1..ad2e4ea9 100644 --- a/README.md +++ b/README.md @@ -17,7 +17,7 @@ ## Introduction -**nfcore/chipseq** is a bioinformatics analysis pipeline used for Chromatin ImmunopreciPitation sequencing (ChIP-seq) data. +**nfcore/chipseq** is a bioinformatics analysis pipeline used for Chromatin ImmunoPrecipitation sequencing (ChIP-seq) data. On release, automated continuous integration tests run the pipeline on a [full-sized dataset](https://github.com/nf-core/test-datasets/tree/chipseq#full-test-dataset-origin) on the AWS cloud infrastructure. The dataset consists of FoxA1 (transcription factor) and EZH2 (histone,mark) IP experiments from _Franco et al. 2015_ ([GEO: GSE59530](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE59530), [PMID: 25752574](https://pubmed.ncbi.nlm.nih.gov/25752574/)) and _Popovic et al. 2014_ ([GEO: GSE57632](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE57632), [PMID: 25188243](https://pubmed.ncbi.nlm.nih.gov/25188243/)), respectively. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from running the full-sized tests can be viewed on the [nf-core website](https://nf-co.re/chipseq/results). diff --git a/conf/modules.config b/conf/modules.config index e374f867..f5f8394c 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -550,10 +550,7 @@ process { params.aligner == "chromap" ? "--format BAM" : '' //TODO check if not needed anymore with new chromap versions ].join(' ').trim() publishDir = [ - path: { [ - "${params.outdir}/${params.aligner}/merged_library/macs2", - params.narrow_peak? '/narrow_peak' : '/broad_peak' - ].join('') }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -562,14 +559,14 @@ process { withName: 'FRIP_SCORE' { ext.args = '-bed -c -f 0.20' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/qc" }, enabled: false ] } withName: 'MULTIQC_CUSTOM_PEAKS' { publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -582,7 +579,7 @@ if (!params.skip_peak_annotation) { ext.args = '-gid' ext.prefix = { "${meta.id}_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -594,7 +591,7 @@ if (!params.skip_peak_annotation) { withName: 'PLOT_MACS2_QC' { ext.args = '-o ./ -p macs2_peak' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -604,7 +601,7 @@ if (!params.skip_peak_annotation) { ext.args = '-o ./' ext.prefix = 'macs2_annotatePeaks' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -619,7 +616,7 @@ if (!params.skip_consensus_peaks) { ext.when = { meta.multiple_groups || meta.replicates_exist } ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -629,7 +626,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-F SAF -O --fracOverlap 0.2' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -642,7 +639,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-gid' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -651,7 +648,7 @@ if (!params.skip_consensus_peaks) { withName: 'ANNOTATE_BOOLEAN_PEAKS' { ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -671,7 +668,7 @@ if (!params.skip_consensus_peaks) { ].join(' ').trim() ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}/deseq2" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}/consensus/${meta.id}/deseq2" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -685,7 +682,7 @@ if (!params.skip_igv) { withName: 'IGV' { publishDir = [ [ - path: { "${params.outdir}/igv/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, + path: { "${params.outdir}/igv/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}" }, mode: params.publish_dir_mode, pattern: '*.{txt,xml}' ], @@ -704,7 +701,7 @@ if (!params.skip_multiqc) { withName: 'MULTIQC' { ext.args = params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' publishDir = [ - path: { "${params.outdir}/multiqc/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, + path: { "${params.outdir}/multiqc/${params.narrow_peak ? 'narrow_peak' : 'broad_peak'}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf index e3a38432..6b9b8efe 100644 --- a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf +++ b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf @@ -30,7 +30,7 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { ch_peaks .map { meta, peak -> - [ meta.antibody, meta.id.split('_')[0..-2].join('_'), peak ] + [ meta.antibody, meta.id - ~/_T\d+$/, peak ] } .groupTuple() .map { diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 6f722b6f..54343de6 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -7,8 +7,6 @@ // // MODULE: Loaded from modules/local/ // -include { MACS2_CONSENSUS } from '../modules/local/macs2_consensus' -include { DESEQ2_QC } from '../modules/local/deseq2_qc' include { IGV } from '../modules/local/igv' include { MULTIQC } from '../modules/local/multiqc' include { MULTIQC_CUSTOM_PHANTOMPEAKQUALTOOLS } from '../modules/local/multiqc_custom_phantompeakqualtools' @@ -46,9 +44,6 @@ include { DEEPTOOLS_PLOTPROFILE } from '../modules/nf-core/deeptools/plo include { DEEPTOOLS_PLOTHEATMAP } from '../modules/nf-core/deeptools/plotheatmap/main' include { DEEPTOOLS_PLOTFINGERPRINT } from '../modules/nf-core/deeptools/plotfingerprint/main' include { KHMER_UNIQUEKMERS } from '../modules/nf-core/khmer/uniquekmers/main' -include { SUBREAD_FEATURECOUNTS } from '../modules/nf-core/subread/featurecounts/main' - -include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../modules/nf-core/homer/annotatepeaks/main' // // SUBWORKFLOW: Consisting entirely of nf-core/modules @@ -240,13 +235,13 @@ workflow CHIPSEQ { meta, bam -> def meta_clone = meta.clone() meta_clone.remove('read_group') - meta_clone.id = meta_clone.id.split('_')[0..-2].join('_') + meta_clone.id = meta_clone.id - ~/_T\d+$/ [ meta_clone, bam ] } .groupTuple(by: [0]) .map { - it -> - [ it[0], it[1].flatten() ] + meta, bam -> + [ meta, bam.flatten() ] } .set { ch_sort_bam } From 2936122cc1522c6fa52d777bfc140cee38d784d7 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 22 Jul 2024 22:17:00 +0200 Subject: [PATCH 179/201] Install macs3 module --- modules.json | 5 + .../nf-core/macs3/callpeak/environment.yml | 9 + modules/nf-core/macs3/callpeak/main.nf | 70 ++++ modules/nf-core/macs3/callpeak/meta.yml | 73 ++++ .../nf-core/macs3/callpeak/tests/bam.config | 5 + .../nf-core/macs3/callpeak/tests/bed.config | 5 + .../nf-core/macs3/callpeak/tests/main.nf.test | 113 ++++++ .../macs3/callpeak/tests/main.nf.test.snap | 358 ++++++++++++++++++ 8 files changed, 638 insertions(+) create mode 100644 modules/nf-core/macs3/callpeak/environment.yml create mode 100644 modules/nf-core/macs3/callpeak/main.nf create mode 100644 modules/nf-core/macs3/callpeak/meta.yml create mode 100644 modules/nf-core/macs3/callpeak/tests/bam.config create mode 100644 modules/nf-core/macs3/callpeak/tests/bed.config create mode 100644 modules/nf-core/macs3/callpeak/tests/main.nf.test create mode 100644 modules/nf-core/macs3/callpeak/tests/main.nf.test.snap diff --git a/modules.json b/modules.json index f4d4950b..33567a0b 100644 --- a/modules.json +++ b/modules.json @@ -90,6 +90,11 @@ "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", "installed_by": ["modules"] }, + "macs3/callpeak": { + "branch": "master", + "git_sha": "6046db922ee6c425a4dff26eeda122dffc3df9c0", + "installed_by": ["modules"] + }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", diff --git a/modules/nf-core/macs3/callpeak/environment.yml b/modules/nf-core/macs3/callpeak/environment.yml new file mode 100644 index 00000000..d9caa561 --- /dev/null +++ b/modules/nf-core/macs3/callpeak/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "macs3_callpeak" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::macs3=3.0.1" diff --git a/modules/nf-core/macs3/callpeak/main.nf b/modules/nf-core/macs3/callpeak/main.nf new file mode 100644 index 00000000..53a1a200 --- /dev/null +++ b/modules/nf-core/macs3/callpeak/main.nf @@ -0,0 +1,70 @@ + +process MACS3_CALLPEAK { + tag "$meta.id" + label 'process_medium' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/macs3:3.0.1--py311h0152c62_3': + 'biocontainers/macs3:3.0.1--py311h0152c62_3' }" + + input: + tuple val(meta), path(ipbam), path(controlbam) + val macs3_gsize + + output: + tuple val(meta), path("*.{narrowPeak,broadPeak}"), emit: peak + tuple val(meta), path("*.xls") , emit: xls + path "versions.yml" , emit: versions + + tuple val(meta), path("*.gappedPeak"), optional:true, emit: gapped + tuple val(meta), path("*.bed") , optional:true, emit: bed + tuple val(meta), path("*.bdg") , optional:true, emit: bdg + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def args_list = args.tokenize() + def format = meta.single_end ? 'BAM' : 'BAMPE' + def control = controlbam ? "--control $controlbam" : '' + if(args_list.contains('--format')){ + def id = args_list.findIndexOf{it=='--format'} + format = args_list[id+1] + args_list.remove(id+1) + args_list.remove(id) + } + """ + macs3 \\ + callpeak \\ + ${args_list.join(' ')} \\ + --gsize $macs3_gsize \\ + --format $format \\ + --name $prefix \\ + --treatment $ipbam \\ + $control + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + macs3: \$(macs3 --version | sed -e "s/macs3 //g") + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.gappedPeak + touch ${prefix}.bed + touch ${prefix}.bdg + touch ${prefix}.narrowPeak + touch ${prefix}.xls + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + macs3: \$(macs3 --version | sed -e "s/macs3 //g") + END_VERSIONS + """ +} diff --git a/modules/nf-core/macs3/callpeak/meta.yml b/modules/nf-core/macs3/callpeak/meta.yml new file mode 100644 index 00000000..b1c7cade --- /dev/null +++ b/modules/nf-core/macs3/callpeak/meta.yml @@ -0,0 +1,73 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "macs3_callpeak" +description: Peak calling of enriched genomic regions of ChIP-seq and ATAC-seq experiments +keywords: + - alignment + - atac-seq + - chip-seq + - peak-calling +tools: + - macs3: + description: "Model Based Analysis for ChIP-Seq data" + homepage: "https://macs3-project.github.io/MACS/" + documentation: "https://macs3-project.github.io/MACS/" + tool_dev_url: "https://github.com/macs3-project/MACS/" + doi: "10.1101/496521" + licence: ["BSD-3-clause"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample_1', single_end:false ]` + - ipbam: + type: file + description: The ChIP-seq treatment file + - controlbam: + type: file + description: The control file + - macs2_gsize: + type: string + description: | + Effective genome size. It can be 1.0e+9 or 1000000000, + or shortcuts:'hs' for human (2,913,022,398), 'mm' for mouse + (2,652,783,500), 'ce' for C. elegans (100,286,401) + and 'dm' for fruitfly (142,573,017), Default:hs. + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - versions: + type: file + description: File containing software version + pattern: "versions.yml" + - peak: + type: file + description: BED file containing annotated peaks + pattern: "*.gappedPeak,*.narrowPeak}" + - xls: + type: file + description: xls file containing annotated peaks + pattern: "*.xls" + - gapped: + type: file + description: Optional BED file containing gapped peak + pattern: "*.gappedPeak" + - bed: + type: file + description: Optional BED file containing peak summits locations for every peak + pattern: "*.bed" + - bdg: + type: file + description: Optional bedGraph files for input and treatment input samples + pattern: "*.bdg" + +authors: + - "@JoseEspinosa" +maintainers: + - "@JoseEspinosa" diff --git a/modules/nf-core/macs3/callpeak/tests/bam.config b/modules/nf-core/macs3/callpeak/tests/bam.config new file mode 100644 index 00000000..217e3107 --- /dev/null +++ b/modules/nf-core/macs3/callpeak/tests/bam.config @@ -0,0 +1,5 @@ +process { + withName: 'MACS3_CALLPEAK' { + ext.args = '--qval 0.1' + } +} \ No newline at end of file diff --git a/modules/nf-core/macs3/callpeak/tests/bed.config b/modules/nf-core/macs3/callpeak/tests/bed.config new file mode 100644 index 00000000..19444006 --- /dev/null +++ b/modules/nf-core/macs3/callpeak/tests/bed.config @@ -0,0 +1,5 @@ +process { + withName: 'MACS3_CALLPEAK' { + ext.args = '--format BED --qval 10 --nomodel --extsize 200' + } +} \ No newline at end of file diff --git a/modules/nf-core/macs3/callpeak/tests/main.nf.test b/modules/nf-core/macs3/callpeak/tests/main.nf.test new file mode 100644 index 00000000..4338c96b --- /dev/null +++ b/modules/nf-core/macs3/callpeak/tests/main.nf.test @@ -0,0 +1,113 @@ +nextflow_process { + + name "Test Process MACS3_CALLPEAK" + script "../main.nf" + process "MACS3_CALLPEAK" + + tag "modules" + tag "modules_nfcore" + tag "macs3" + tag "macs3/callpeak" + + test("homo_sapiens - callpeak - bed") { + + when { + config "./bed.config" + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/pacbio/bed/alz.ccs.fl.NEB_5p--NEB_Clontech_3p.flnc.clustered.singletons.merged.aligned_tc.bed', checkIfExists: true) ], + [] + ] + input[1] = 4000 + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - callpeak - bam") { + + when { + config "./bam.config" + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.name.sorted.bam', checkIfExists: true) ], + [] + ] + input[1] = 40000 + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - callpeak - control - bam") { + + when { + config "./bam.config" + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test.paired_end.name.sorted.bam', checkIfExists: true) ], + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/bam/test2.paired_end.name.sorted.bam', checkIfExists: true) ] + ] + input[1] = 40000 + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2 - bam - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) ], + [] + ] + input[1] = 40000 + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/macs3/callpeak/tests/main.nf.test.snap b/modules/nf-core/macs3/callpeak/tests/main.nf.test.snap new file mode 100644 index 00000000..d6d98292 --- /dev/null +++ b/modules/nf-core/macs3/callpeak/tests/main.nf.test.snap @@ -0,0 +1,358 @@ +{ + "homo_sapiens - callpeak - bam": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.narrowPeak:md5,2e4da1c1704595e12aaf99cc715ad70c" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.xls:md5,221852e4639574d2f53cf1917efa4922" + ] + ], + "2": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "3": [ + + ], + "4": [ + [ + { + "id": "test", + "single_end": false + }, + "test_summits.bed:md5,26f0f97b6c14dbca129e947a58067c82" + ] + ], + "5": [ + + ], + "bdg": [ + + ], + "bed": [ + [ + { + "id": "test", + "single_end": false + }, + "test_summits.bed:md5,26f0f97b6c14dbca129e947a58067c82" + ] + ], + "gapped": [ + + ], + "peak": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.narrowPeak:md5,2e4da1c1704595e12aaf99cc715ad70c" + ] + ], + "versions": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "xls": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.xls:md5,221852e4639574d2f53cf1917efa4922" + ] + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-22T17:04:31.629715" + }, + "sarscov2 - bam - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test.narrowPeak:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test.xls:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "3": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gappedPeak:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "4": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "5": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bdg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "bdg": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bdg:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "bed": [ + [ + { + "id": "test", + "single_end": false + }, + "test.bed:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "gapped": [ + [ + { + "id": "test", + "single_end": false + }, + "test.gappedPeak:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "peak": [ + [ + { + "id": "test", + "single_end": false + }, + "test.narrowPeak:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "xls": [ + [ + { + "id": "test", + "single_end": false + }, + "test.xls:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-22T17:04:58.589844" + }, + "homo_sapiens - callpeak - control - bam": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.narrowPeak:md5,653e1108cc57ca07d0f60fc0f4fb8ba3" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.xls:md5,9fce04613bdc9c8372a9f884aa0d5aa6" + ] + ], + "2": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "3": [ + + ], + "4": [ + [ + { + "id": "test", + "single_end": false + }, + "test_summits.bed:md5,4f3c7c53a1d730d90d1b3dd9d3197af4" + ] + ], + "5": [ + + ], + "bdg": [ + + ], + "bed": [ + [ + { + "id": "test", + "single_end": false + }, + "test_summits.bed:md5,4f3c7c53a1d730d90d1b3dd9d3197af4" + ] + ], + "gapped": [ + + ], + "peak": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.narrowPeak:md5,653e1108cc57ca07d0f60fc0f4fb8ba3" + ] + ], + "versions": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "xls": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.xls:md5,9fce04613bdc9c8372a9f884aa0d5aa6" + ] + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-22T17:04:44.063426" + }, + "homo_sapiens - callpeak - bed": { + "content": [ + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.narrowPeak:md5,10e7d4747f8a2513e5ebb04856a51673" + ] + ], + "1": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.xls:md5,14efbf7137623df5aaf282b506ac9601" + ] + ], + "2": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "3": [ + + ], + "4": [ + [ + { + "id": "test", + "single_end": false + }, + "test_summits.bed:md5,28833eeb7816688f0d698f51670be946" + ] + ], + "5": [ + + ], + "bdg": [ + + ], + "bed": [ + [ + { + "id": "test", + "single_end": false + }, + "test_summits.bed:md5,28833eeb7816688f0d698f51670be946" + ] + ], + "gapped": [ + + ], + "peak": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.narrowPeak:md5,10e7d4747f8a2513e5ebb04856a51673" + ] + ], + "versions": [ + "versions.yml:md5,cb33970f9aaa0730733abe2fd9cb2b74" + ], + "xls": [ + [ + { + "id": "test", + "single_end": false + }, + "test_peaks.xls:md5,14efbf7137623df5aaf282b506ac9601" + ] + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.04.2" + }, + "timestamp": "2024-07-22T17:04:16.697163" + } +} \ No newline at end of file From dabbe8cf77c6a0a06358f14c23d53f40481cffe5 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Mon, 22 Jul 2024 22:31:48 +0200 Subject: [PATCH 180/201] Remove macs2/callpeak module --- modules.json | 198 ++++++++++++++++++------ modules/nf-core/macs2/callpeak/main.nf | 53 ------- modules/nf-core/macs2/callpeak/meta.yml | 63 -------- 3 files changed, 148 insertions(+), 166 deletions(-) delete mode 100644 modules/nf-core/macs2/callpeak/main.nf delete mode 100644 modules/nf-core/macs2/callpeak/meta.yml diff --git a/modules.json b/modules.json index 33567a0b..a1bfa4c7 100644 --- a/modules.json +++ b/modules.json @@ -8,177 +8,252 @@ "bowtie2/align": { "branch": "master", "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", - "installed_by": ["fastq_align_bowtie2", "modules"] + "installed_by": [ + "fastq_align_bowtie2", + "modules" + ] }, "bowtie2/build": { "branch": "master", "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "bwa/mem": { "branch": "master", "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", - "installed_by": ["fastq_align_bwa", "modules"] + "installed_by": [ + "fastq_align_bwa", + "modules" + ] }, "chromap/chromap": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["fastq_align_chromap", "modules"] + "installed_by": [ + "fastq_align_chromap", + "modules" + ] }, "chromap/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/getchromsizes": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/computematrix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "gffread": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] - }, - "macs2/callpeak": { - "branch": "master", - "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "macs3/callpeak": { "branch": "master", "git_sha": "6046db922ee6c425a4dff26eeda122dffc3df9c0", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "phantompeakqualtools": { "branch": "master", "git_sha": "2dfe9afa90fefc70e320140e5f41287f01f324b0", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "picard/markduplicates": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": ["bam_markduplicates_picard", "modules"] + "installed_by": [ + "bam_markduplicates_picard", + "modules" + ] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "preseq/lcextrap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "samtools/flagstat": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_stats_samtools", "modules"] + "installed_by": [ + "bam_stats_samtools", + "modules" + ] }, "samtools/idxstats": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_stats_samtools", "modules"] + "installed_by": [ + "bam_stats_samtools", + "modules" + ] }, "samtools/index": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools", "modules"] + "installed_by": [ + "bam_markduplicates_picard", + "bam_sort_stats_samtools", + "modules" + ] }, "samtools/sort": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": ["bam_sort_stats_samtools", "modules"] + "installed_by": [ + "bam_sort_stats_samtools", + "modules" + ] }, "samtools/stats": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": ["bam_stats_samtools", "modules"] + "installed_by": [ + "bam_stats_samtools", + "modules" + ] }, "subread/featurecounts": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "trimgalore": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore", + "modules" + ] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "umitools/extract": { "branch": "master", "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", - "installed_by": ["fastq_fastqc_umitools_trimgalore"] + "installed_by": [ + "fastq_fastqc_umitools_trimgalore" + ] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "untarfiles": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -187,55 +262,78 @@ "bam_markduplicates_picard": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "bam_sort_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["fastq_align_bowtie2", "fastq_align_bwa", "fastq_align_chromap"] + "installed_by": [ + "fastq_align_bowtie2", + "fastq_align_bwa", + "fastq_align_chromap" + ] }, "bam_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] + "installed_by": [ + "bam_markduplicates_picard", + "bam_sort_stats_samtools" + ] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "fastq_align_bwa": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "fastq_align_chromap": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/macs2/callpeak/main.nf b/modules/nf-core/macs2/callpeak/main.nf deleted file mode 100644 index cbef7838..00000000 --- a/modules/nf-core/macs2/callpeak/main.nf +++ /dev/null @@ -1,53 +0,0 @@ -process MACS2_CALLPEAK { - tag "$meta.id" - label 'process_medium' - - conda "bioconda::macs2=2.2.7.1" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/macs2:2.2.7.1--py38h4a8c8d9_3' : - 'biocontainers/macs2:2.2.7.1--py38h4a8c8d9_3' }" - - input: - tuple val(meta), path(ipbam), path(controlbam) - val macs2_gsize - - output: - tuple val(meta), path("*.{narrowPeak,broadPeak}"), emit: peak - tuple val(meta), path("*.xls") , emit: xls - path "versions.yml" , emit: versions - - tuple val(meta), path("*.gappedPeak"), optional:true, emit: gapped - tuple val(meta), path("*.bed") , optional:true, emit: bed - tuple val(meta), path("*.bdg") , optional:true, emit: bdg - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - def args_list = args.tokenize() - def format = meta.single_end ? 'BAM' : 'BAMPE' - def control = controlbam ? "--control $controlbam" : '' - if(args_list.contains('--format')){ - def id = args_list.findIndexOf{it=='--format'} - format = args_list[id+1] - args_list.remove(id+1) - args_list.remove(id) - } - """ - macs2 \\ - callpeak \\ - ${args_list.join(' ')} \\ - --gsize $macs2_gsize \\ - --format $format \\ - --name $prefix \\ - --treatment $ipbam \\ - $control - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - macs2: \$(macs2 --version | sed -e "s/macs2 //g") - END_VERSIONS - """ -} diff --git a/modules/nf-core/macs2/callpeak/meta.yml b/modules/nf-core/macs2/callpeak/meta.yml deleted file mode 100644 index 6e2bc1db..00000000 --- a/modules/nf-core/macs2/callpeak/meta.yml +++ /dev/null @@ -1,63 +0,0 @@ -name: macs2_callpeak -description: Peak calling of enriched genomic regions of ChIP-seq and ATAC-seq experiments -keywords: - - alignment - - atac-seq - - chip-seq - - peak-calling -tools: - - macs2: - description: Model Based Analysis for ChIP-Seq data - - documentation: https://docs.csc.fi/apps/macs2/ - tool_dev_url: https://github.com/macs3-project/MACS - doi: "10.1101/496521" - licence: ["BSD"] - -input: - - meta: - type: map - description: | - Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] - - ipbam: - type: file - description: The ChIP-seq treatment file - - controlbam: - type: file - description: The control file - - macs2_gsize: - type: string - description: Effective genome size. It can be 1.0e+9 or 1000000000, or shortcuts:'hs' for human (2.7e9), - 'mm' for mouse (1.87e9), 'ce' for C. elegans (9e7) and 'dm' for fruitfly (1.2e8) - -output: - - versions: - type: file - description: File containing software version - pattern: "versions.yml" - - peak: - type: file - description: BED file containing annotated peaks - pattern: "*.gappedPeak,*.narrowPeak}" - - xls: - type: file - description: xls file containing annotated peaks - pattern: "*.xls" - - gapped: - type: file - description: Optional BED file containing gapped peak - pattern: "*.gappedPeak" - - bed: - type: file - description: Optional BED file containing peak summits locations for every peak - pattern: "*.bed" - - bdg: - type: file - description: Optional bedGraph files for input and treatment input samples - pattern: "*.bdg" - -authors: - - "@ntoda03" - - "@JoseEspinosa" - - "@jianhong" From 01623d6f403ee03d188dd79bddd04f217b1215a8 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 23 Jul 2024 11:13:34 +0200 Subject: [PATCH 181/201] Update all code using macs2 to use macs3, also docs --- CITATIONS.md | 2 +- README.md | 2 +- assets/multiqc/frip_score_header.txt | 4 +- assets/multiqc/peak_count_header.txt | 4 +- assets/multiqc_config.yml | 2 +- assets/schema_input.json | 19 +++++- ...erged_expand.py => macs3_merged_expand.py} | 10 +-- bin/{plot_macs2_qc.r => plot_macs3_qc.r} | 2 +- conf/modules.config | 34 +++++----- docs/output.md | 24 +++---- modules/local/igv.nf | 6 +- ...{macs2_consensus.nf => macs3_consensus.nf} | 4 +- modules/local/multiqc.nf | 8 +-- .../{plot_macs2_qc.nf => plot_macs3_qc.nf} | 4 +- nextflow_schema.json | 20 +++--- .../utils_nfcore_chipseq_pipeline/main.nf | 4 +- test.csv | 7 ++ tower.yml | 10 +-- workflows/chipseq.nf | 66 +++++++++---------- 19 files changed, 127 insertions(+), 105 deletions(-) rename bin/{macs2_merged_expand.py => macs3_merged_expand.py} (96%) rename bin/{plot_macs2_qc.r => plot_macs3_qc.r} (99%) rename modules/local/{macs2_consensus.nf => macs3_consensus.nf} (98%) rename modules/local/{plot_macs2_qc.nf => plot_macs3_qc.nf} (96%) create mode 100644 test.csv diff --git a/CITATIONS.md b/CITATIONS.md index f8160c8f..d75a5154 100644 --- a/CITATIONS.md +++ b/CITATIONS.md @@ -46,7 +46,7 @@ > Heinz S, Benner C, Spann N, Bertolino E, Lin YC, Laslo P, Cheng JX, Murre C, Singh H, Glass CK. Simple combinations of lineage-determining transcription factors prime cis-regulatory elements required for macrophage and B cell identities. Mol Cell. 2010 May 28;38(4):576-89. doi: 10.1016/j.molcel.2010.05.004. PubMed PMID: 20513432; PubMed Central PMCID: PMC2898526. -- [MACS2](https://www.ncbi.nlm.nih.gov/pubmed/18798982/) +- [MACS3](https://www.ncbi.nlm.nih.gov/pubmed/18798982/) > Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17. PubMed PMID: 18798982; PubMed Central PMCID: PMC2592715. diff --git a/README.md b/README.md index 2dcb70e1..01a8a10c 100644 --- a/README.md +++ b/README.md @@ -59,7 +59,7 @@ You can find numerous talks on the [nf-core events page](https://nf-co.re/events 5. Generate gene-body meta-profile from bigWig files ([`deepTools`](https://deeptools.readthedocs.io/en/develop/content/tools/plotProfile.html)) 6. Calculate genome-wide IP enrichment relative to control ([`deepTools`](https://deeptools.readthedocs.io/en/develop/content/tools/plotFingerprint.html)) 7. Calculate strand cross-correlation peak and ChIP-seq quality measures including NSC and RSC ([`phantompeakqualtools`](https://github.com/kundajelab/phantompeakqualtools)) - 8. Call broad/narrow peaks ([`MACS2`](https://github.com/macs3-project/MACS)) + 8. Call broad/narrow peaks ([`MACS3`](https://github.com/macs3-project/MACS)) 9. Annotate peaks relative to gene features ([`HOMER`](http://homer.ucsd.edu/homer/download.html)) 10. Create consensus peakset across all samples and create tabular file to aid in the filtering of the data ([`BEDTools`](https://github.com/arq5x/bedtools2/)) 11. Count reads in consensus peaks ([`featureCounts`](http://bioinf.wehi.edu.au/featureCounts/)) diff --git a/assets/multiqc/frip_score_header.txt b/assets/multiqc/frip_score_header.txt index 82902115..b8c35cd7 100644 --- a/assets/multiqc/frip_score_header.txt +++ b/assets/multiqc/frip_score_header.txt @@ -1,7 +1,7 @@ #id: 'frip_score' -#section_name: 'MERGED LIB: MACS2 FRiP score' +#section_name: 'MERGED LIB: MACS3 FRiP score' #description: "is generated by calculating the fraction of all mapped reads that fall -# into the MACS2 called peak regions. A read must overlap a peak by at least 20% to be counted. +# into the MACS3 called peak regions. A read must overlap a peak by at least 20% to be counted. # See FRiP score." #plot_type: 'bargraph' #anchor: 'frip_score' diff --git a/assets/multiqc/peak_count_header.txt b/assets/multiqc/peak_count_header.txt index aa4dd346..66f6d2d9 100644 --- a/assets/multiqc/peak_count_header.txt +++ b/assets/multiqc/peak_count_header.txt @@ -1,7 +1,7 @@ #id: 'peak_count' -#section_name: 'MERGED LIB: MACS2 peak count' +#section_name: 'MERGED LIB: MACS3 peak count' #description: "is calculated from total number of peaks called by -# MACS2" +# MACS3" #plot_type: 'bargraph' #anchor: 'peak_count' #pconfig: diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 14a9f451..4f32837d 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -72,7 +72,7 @@ module_order: anchor: "mlib_featurecounts" info: "This section of the report shows featureCounts results for the number of reads assigned to merged library consensus peaks." path_filters: - - "./macs2/featurecounts/*.summary" + - "./macs3/featurecounts/*.summary" report_section_order: peak_count: diff --git a/assets/schema_input.json b/assets/schema_input.json index bc2fa467..fb41210f 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -33,15 +33,30 @@ } ] }, + "replicate": { + "type": "integer", + "errorMessage": "Replicate id not an integer!", + "meta": ["replicate"] + }, "antibody": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Antibody entry cannot contain spaces" + "errorMessage": "Antibody entry cannot contain spaces", + "dependentRequired": ["control"], + "meta": ["antibody"] }, "control": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Control entry cannot contain spaces" + "errorMessage": "Control entry cannot contain spaces", + "dependentRequired": ["antibody", "control_replicate"], + "meta": ["control"] + }, + "control_replicate": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Control entry cannot contain spaces", + "meta": ["control_replicate"] } }, "required": ["sample", "fastq_1"] diff --git a/bin/macs2_merged_expand.py b/bin/macs3_merged_expand.py similarity index 96% rename from bin/macs2_merged_expand.py rename to bin/macs3_merged_expand.py index 28ffb087..64dc7770 100755 --- a/bin/macs2_merged_expand.py +++ b/bin/macs3_merged_expand.py @@ -17,15 +17,15 @@ ############################################ Description = "Add sample boolean files and aggregate columns from merged MACS narrow or broad peak file." -Epilog = """Example usage: python macs2_merged_expand.py --is_narrow_peak --min_replicates 1""" +Epilog = """Example usage: python macs3_merged_expand.py --is_narrow_peak --min_replicates 1""" argParser = argparse.ArgumentParser(description=Description, epilog=Epilog) ## REQUIRED PARAMETERS -argParser.add_argument("MERGED_INTERVAL_FILE", help="Merged MACS2 interval file created using linux sort and mergeBed.") +argParser.add_argument("MERGED_INTERVAL_FILE", help="Merged MACS3 interval file created using linux sort and mergeBed.") argParser.add_argument( "SAMPLE_NAME_LIST", - help="Comma-separated list of sample names as named in individual MACS2 broadPeak/narrowPeak output file e.g. SAMPLE_R1 for SAMPLE_R1_peak_1.", + help="Comma-separated list of sample names as named in individual MACS3 broadPeak/narrowPeak output file e.g. SAMPLE_R1 for SAMPLE_R1_peak_1.", ) argParser.add_argument("OUTFILE", help="Full path to output directory.") @@ -76,7 +76,7 @@ def makedir(path): ## sort -k1,1 -k2,2n | mergeBed -c 2,3,4,5,6,7,8,9,10 -o collapse,collapse,collapse,collapse,collapse,collapse,collapse,collapse,collapse > merged_peaks.txt -def macs2_merged_expand(MergedIntervalTxtFile, SampleNameList, OutFile, isNarrow=False, minReplicates=1): +def macs3_merged_expand(MergedIntervalTxtFile, SampleNameList, OutFile, isNarrow=False, minReplicates=1): makedir(os.path.dirname(OutFile)) combFreqDict = {} @@ -208,7 +208,7 @@ def macs2_merged_expand(MergedIntervalTxtFile, SampleNameList, OutFile, isNarrow ############################################ ############################################ -macs2_merged_expand( +macs3_merged_expand( MergedIntervalTxtFile=args.MERGED_INTERVAL_FILE, SampleNameList=args.SAMPLE_NAME_LIST.split(","), OutFile=args.OUTFILE, diff --git a/bin/plot_macs2_qc.r b/bin/plot_macs3_qc.r similarity index 99% rename from bin/plot_macs2_qc.r rename to bin/plot_macs3_qc.r index 5cf074de..e40a6837 100755 --- a/bin/plot_macs2_qc.r +++ b/bin/plot_macs3_qc.r @@ -20,7 +20,7 @@ library(scales) option_list <- list(make_option(c("-i", "--peak_files"), type="character", default=NULL, help="Comma-separated list of peak files.", metavar="path"), make_option(c("-s", "--sample_ids"), type="character", default=NULL, help="Comma-separated list of sample ids associated with peak files. Must be unique and in same order as peaks files input.", metavar="string"), make_option(c("-o", "--outdir"), type="character", default='./', help="Output directory", metavar="path"), - make_option(c("-p", "--outprefix"), type="character", default='macs2_peakqc', help="Output prefix", metavar="string")) + make_option(c("-p", "--outprefix"), type="character", default='macs3_peakqc', help="Output prefix", metavar="string")) opt_parser <- OptionParser(option_list=option_list) opt <- parse_args(opt_parser) diff --git a/conf/modules.config b/conf/modules.config index 59af9e66..8828f48f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -540,7 +540,7 @@ if (!params.skip_plot_fingerprint) { } process { - withName: 'MACS2_CALLPEAK' { + withName: 'MACS3_CALLPEAK' { ext.args = [ '--keep-dup all', params.narrow_peak ? '' : "--broad --broad-cutoff ${params.broad_cutoff}", @@ -551,7 +551,7 @@ process { ].join(' ').trim() publishDir = [ path: { [ - "${params.outdir}/${params.aligner}/merged_library/macs2", + "${params.outdir}/${params.aligner}/merged_library/macs3", params.narrow_peak? '/narrow_peak' : '/broad_peak' ].join('') }, mode: params.publish_dir_mode, @@ -562,14 +562,14 @@ process { withName: 'FRIP_SCORE' { ext.args = '-bed -c -f 0.20' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, enabled: false ] } withName: 'MULTIQC_CUSTOM_PEAKS' { publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -578,11 +578,11 @@ process { if (!params.skip_peak_annotation) { process { - withName: 'HOMER_ANNOTATEPEAKS_MACS2' { + withName: 'HOMER_ANNOTATEPEAKS_MACS3' { ext.args = '-gid' ext.prefix = { "${meta.id}_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -591,10 +591,10 @@ if (!params.skip_peak_annotation) { if (!params.skip_peak_qc) { process { - withName: 'PLOT_MACS2_QC' { - ext.args = '-o ./ -p macs2_peak' + withName: 'PLOT_MACS3_QC' { + ext.args = '-o ./ -p macs3_peak' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -602,9 +602,9 @@ if (!params.skip_peak_annotation) { withName: 'PLOT_HOMER_ANNOTATEPEAKS' { ext.args = '-o ./' - ext.prefix = 'macs2_annotatePeaks' + ext.prefix = 'macs3_annotatePeaks' publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/qc" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -615,11 +615,11 @@ if (!params.skip_peak_annotation) { if (!params.skip_consensus_peaks) { process { - withName: 'MACS2_CONSENSUS' { + withName: 'MACS3_CONSENSUS' { ext.when = { meta.multiple_groups || meta.replicates_exist } ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -629,7 +629,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-F SAF -O --fracOverlap 0.2' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -642,7 +642,7 @@ if (!params.skip_consensus_peaks) { ext.args = '-gid' ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -651,7 +651,7 @@ if (!params.skip_consensus_peaks) { withName: 'ANNOTATE_BOOLEAN_PEAKS' { ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] @@ -671,7 +671,7 @@ if (!params.skip_consensus_peaks) { ].join(' ').trim() ext.prefix = { "${meta.id}.consensus_peaks" } publishDir = [ - path: { "${params.outdir}/${params.aligner}/merged_library/macs2/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}/deseq2" }, + path: { "${params.outdir}/${params.aligner}/merged_library/macs3/${params.narrow_peak ? '/narrow_peak' : '/broad_peak'}/consensus/${meta.id}/deseq2" }, mode: params.publish_dir_mode, saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] diff --git a/docs/output.md b/docs/output.md index d8131c61..2f0ee320 100644 --- a/docs/output.md +++ b/docs/output.md @@ -188,21 +188,21 @@ The results from deepTools plotProfile gives you a quick visualisation for the g
    Output files -- `/merged_library/macs2//` - - `*.xls`, `*.broadPeak` or `*.narrowPeak`, `*.gappedPeak`, `*summits.bed`: MACS2 output files - the files generated will depend on whether MACS2 has been run in _narrowPeak_ or _broadPeak_ mode. +- `/merged_library/macs3//` + - `*.xls`, `*.broadPeak` or `*.narrowPeak`, `*.gappedPeak`, `*summits.bed`: MACS3 output files - the files generated will depend on whether MACS3 has been run in _narrowPeak_ or _broadPeak_ mode. - `*.annotatePeaks.txt`: HOMER peak-to-gene annotation file. -- `/merged_library/macs2//qc/` - - `macs2_peak.plots.pdf`: QC plots for MACS2 peaks. - - `macs2_annotatePeaks.plots.pdf`: QC plots for peak-to-gene feature annotation. +- `/merged_library/macs3//qc/` + - `macs3_peak.plots.pdf`: QC plots for MACS3 peaks. + - `macs3_annotatePeaks.plots.pdf`: QC plots for peak-to-gene feature annotation. - `*.FRiP_mqc.tsv`, `*.peak_count_mqc.tsv`, `annotatepeaks.summary_mqc.tsv`: MultiQC custom-content files for FRiP score, peak count and peak-to-gene ratios. -> **NB:** `` in the directory structure above corresponds to the type of peak that you have specified to call with MACS2 i.e. `broadPeak` or `narrowPeak`. If you so wish, you can call both narrow and broad peaks without redoing the preceding steps in the pipeline such as the alignment and filtering. For example, if you already have broad peaks then just add `--narrow_peak -resume` to the command you used to run the pipeline, and these will be called too! However, resuming the pipeline will only be possible if you have not deleted the `work/` directory generated by the pipeline. +> **NB:** `` in the directory structure above corresponds to the type of peak that you have specified to call with MACS3 i.e. `broadPeak` or `narrowPeak`. If you so wish, you can call both narrow and broad peaks without redoing the preceding steps in the pipeline such as the alignment and filtering. For example, if you already have broad peaks then just add `--narrow_peak -resume` to the command you used to run the pipeline, and these will be called too! However, resuming the pipeline will only be possible if you have not deleted the `work/` directory generated by the pipeline.
    -[MACS2](https://github.com/macs3-project/MACS) is one of the most popular peak-calling algorithms for ChIP-seq data. By default, the peaks are called with the MACS2 `--broad` parameter. If, however, you would like to call narrow peaks then please provide the `--narrow_peak` parameter when running the pipeline. See [MACS2 outputs](https://github.com/macs3-project/MACS/blob/master/docs/callpeak.md#output-files) for a description of the output files generated by MACS2. +[MACS3](https://github.com/macs3-project/MACS) is one of the most popular peak-calling algorithms for ChIP-seq data. By default, the peaks are called with the MACS3 `--broad` parameter. If, however, you would like to call narrow peaks then please provide the `--narrow_peak` parameter when running the pipeline. See [MACS3 outputs](https://github.com/macs3-project/MACS/blob/master/docs/callpeak.md#output-files) for a description of the output files generated by MACS3. -![MultiQC - MACS2 total peak count plot](images/mqc_macs2_peak_count_plot.png) +![MultiQC - MACS3 total peak count plot](images/mqc_macs2_peak_count_plot.png) [HOMER annotatePeaks.pl](http://homer.ucsd.edu/homer/ngs/annotation.html) is used to annotate the peaks relative to known genomic features. HOMER is able to use the `--gtf` annotation file which is provided to the pipeline. Please note that some of the output columns will be blank because the annotation is not provided using HOMER's in-built database format. However, the more important fields required for downstream analysis will be populated i.e. _Annotation_, _Distance to TSS_ and _Nearest Promoter ID_. @@ -210,14 +210,14 @@ The results from deepTools plotProfile gives you a quick visualisation for the g Various QC plots per sample including number of peaks, fold-change distribution, [FRiP score](https://genome.cshlp.org/content/22/9/1813.full.pdf+html) and peak-to-gene feature annotation are also generated by the pipeline. Where possible these have been integrated into the MultiQC report. -![MultiQC - MACS2 peaks FRiP score plot](images/mqc_frip_score_plot.png) +![MultiQC - MACS3 peaks FRiP score plot](images/mqc_frip_score_plot.png) ### Create and quantify consensus set of peaks
    Output files -- `/merged_library/macs2//consensus//` +- `/merged_library/macs3//consensus//` - `*.bed`: Consensus peak-set across all samples in BED format. - `*.saf`: Consensus peak-set across all samples in SAF format. Required by featureCounts for read quantification. - `*.featureCounts.txt`: Read counts across all samples relative to consensus peak-set. @@ -245,7 +245,7 @@ The [featureCounts](http://bioinf.wehi.edu.au/featureCounts/) tool is used to co
    Output files -- `/merged_library/macs2//consensus//deseq2/` +- `/merged_library/macs3//consensus//deseq2/` - `*.sample.dists.txt`: Spreadsheet containing sample-to-sample distance across each consensus peak. - `*.plots.pdf`: File containing PCA and hierarchical clustering plots. - `*.dds.RData`: File containing R `DESeqDataSet` object generated by DESeq2, with either @@ -254,7 +254,7 @@ The [featureCounts](http://bioinf.wehi.edu.au/featureCounts/) tool is used to co `readRDS` to give user control of the eventual object name. - `*pca.vals.txt`: Matrix of values for the first 2 principal components. - `R_sessionInfo.log`: File containing information about R, the OS and attached or loaded packages. - - `/merged_library/macs2//consensus//sizeFactors/` + - `/merged_library/macs3//consensus//sizeFactors/` - `*.txt`, `*.RData`: Files containing DESeq2 sizeFactors per sample.
    diff --git a/modules/local/igv.nf b/modules/local/igv.nf index 4352edae..182a339e 100644 --- a/modules/local/igv.nf +++ b/modules/local/igv.nf @@ -13,8 +13,8 @@ process IGV { val peak_dir path fasta path ("${aligner_dir}/merged_library/bigwig/*") - path ("${aligner_dir}/merged_library/macs2/${peak_dir}/*") - path ("${aligner_dir}/merged_library/macs2/${peak_dir}/consensus/*") + path ("${aligner_dir}/merged_library/macs3/${peak_dir}/*") + path ("${aligner_dir}/merged_library/macs3/${peak_dir}/consensus/*") path ("mappings/*") output: @@ -27,7 +27,7 @@ process IGV { task.ext.when == null || task.ext.when script: // scripts are bundled with the pipeline in nf-core/chipseq/bin/ - def consensus_dir = "${aligner_dir}/merged_library/macs2/${peak_dir}/consensus/*" + def consensus_dir = "${aligner_dir}/merged_library/macs3/${peak_dir}/consensus/*" """ find * -type l -name "*.bigWig" -exec echo -e ""{}"\\t0,0,178" \\; > bigwig.igv.txt find * -type l -name "*Peak" -exec echo -e ""{}"\\t0,0,178" \\; > peaks.igv.txt diff --git a/modules/local/macs2_consensus.nf b/modules/local/macs3_consensus.nf similarity index 98% rename from modules/local/macs2_consensus.nf rename to modules/local/macs3_consensus.nf index 6b2006e4..520727a8 100644 --- a/modules/local/macs2_consensus.nf +++ b/modules/local/macs3_consensus.nf @@ -1,7 +1,7 @@ /* * Consensus peaks across samples, create boolean filtering file, SAF file for featureCounts */ -process MACS2_CONSENSUS { +process MACS3_CONSENSUS { tag "$meta.id" label 'process_long' @@ -37,7 +37,7 @@ process MACS2_CONSENSUS { sort -T '.' -k1,1 -k2,2n ${peaks.collect{it.toString()}.sort().join(' ')} \\ | mergeBed -c $mergecols -o $collapsecols > ${prefix}.txt - macs2_merged_expand.py \\ + macs3_merged_expand.py \\ ${prefix}.txt \\ ${peaks.collect{it.toString()}.sort().join(',').replaceAll("_peaks.${peak_type}","")} \\ ${prefix}.boolean.txt \\ diff --git a/modules/local/multiqc.nf b/modules/local/multiqc.nf index 31da0b07..db64f9d7 100644 --- a/modules/local/multiqc.nf +++ b/modules/local/multiqc.nf @@ -40,10 +40,10 @@ process MULTIQC { path ('phantompeakqualtools/*') path ('phantompeakqualtools/*') - path ('macs2/peaks/*') - path ('macs2/peaks/*') - path ('macs2/annotation/*') - path ('macs2/featurecounts/*') + path ('macs3/peaks/*') + path ('macs3/peaks/*') + path ('macs3/annotation/*') + path ('macs3/featurecounts/*') path ('deseq2/*') path ('deseq2/*') diff --git a/modules/local/plot_macs2_qc.nf b/modules/local/plot_macs3_qc.nf similarity index 96% rename from modules/local/plot_macs2_qc.nf rename to modules/local/plot_macs3_qc.nf index d514db46..1722cadd 100644 --- a/modules/local/plot_macs2_qc.nf +++ b/modules/local/plot_macs3_qc.nf @@ -1,4 +1,4 @@ -process PLOT_MACS2_QC { +process PLOT_MACS3_QC { label 'process_medium' conda "conda-forge::r-base=4.0.3 conda-forge::r-reshape2=1.4.4 conda-forge::r-optparse=1.6.6 conda-forge::r-ggplot2=3.3.3 conda-forge::r-scales=1.1.1 conda-forge::r-viridis=0.5.1 conda-forge::r-tidyverse=1.3.0 bioconda::bioconductor-biostrings=2.58.0 bioconda::bioconductor-complexheatmap=2.6.2" @@ -22,7 +22,7 @@ process PLOT_MACS2_QC { def args = task.ext.args ?: '' def peak_type = is_narrow_peak ? 'narrowPeak' : 'broadPeak' """ - plot_macs2_qc.r \\ + plot_macs3_qc.r \\ -i ${peaks.join(',')} \\ -s ${peaks.join(',').replaceAll("_peaks.${peak_type}","")} \\ $args diff --git a/nextflow_schema.json b/nextflow_schema.json index f51e0940..7189751b 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -36,9 +36,9 @@ }, "read_length": { "type": "integer", - "description": "Read length used to calculate MACS2 genome size for peak calling if `--macs_gsize` isn't provided.", + "description": "Read length used to calculate MACS3 genome size for peak calling if `--macs_gsize` isn't provided.", "fa_icon": "fas fa-chart-area", - "help_text": "Read length together with the genome fasta are used to calculate MACS2 genome size using the `khmer` program as explained [here](https://deeptools.readthedocs.io/en/develop/content/feature/effectiveGenomeSize.html#effective-genome-size). For all the genomes present in the `igenomes.config` the genome size has been already precomputed and the read length is then used to retrieve the corresponding value", + "help_text": "Read length together with the genome fasta are used to calculate MACS3 genome size using the `khmer` program as explained [here](https://deeptools.readthedocs.io/en/develop/content/feature/effectiveGenomeSize.html#effective-genome-size). For all the genomes present in the `igenomes.config` the genome size has been already precomputed and the read length is then used to retrieve the corresponding value", "enum": [50, 75, 100, 150, 200] }, "outdir": { @@ -142,8 +142,8 @@ }, "macs_gsize": { "type": "number", - "description": "Effective genome size parameter required by MACS2.", - "help_text": "[Effective genome size](https://github.com/taoliu/MACS#-g--gsize) parameter required by MACS2. If using an iGenomes reference these have been provided for any of the genomes available in the igenomes.config, and for the following read lengths (50,75,100,150,200) that should be set using the `--read_length` parameter. For other genomes, if this parameter is not specified it will be inferred using the provided `--read_length` or otherwise the pipeline execution will stop with an error.", + "description": "Effective genome size parameter required by MACS3.", + "help_text": "[Effective genome size](https://github.com/taoliu/MACS#-g--gsize) parameter required by MACS3. If using an iGenomes reference these have been provided for any of the genomes available in the igenomes.config, and for the following read lengths (50,75,100,150,200) that should be set using the `--read_length` parameter. For other genomes, if this parameter is not specified it will be inferred using the provided `--read_length` or otherwise the pipeline execution will stop with an error.", "fa_icon": "fas fa-arrows-alt-h" }, "blacklist": { @@ -292,14 +292,14 @@ "properties": { "narrow_peak": { "type": "boolean", - "description": "Run MACS2 in narrowPeak mode.", - "help_text": "MACS2 is run by default with the [`--broad`](https://github.com/taoliu/MACS#--broad) flag. Specify this flag to call peaks in narrowPeak mode.", + "description": "Run MACS3 in narrowPeak mode.", + "help_text": "MACS3 is run by default with the [`--broad`](https://github.com/taoliu/MACS#--broad) flag. Specify this flag to call peaks in narrowPeak mode.", "fa_icon": "fas fa-arrows-alt-h" }, "broad_cutoff": { "type": "number", "default": 0.1, - "description": "Specifies broad cutoff value for MACS2. Only used when --narrow_peak isnt specified.", + "description": "Specifies broad cutoff value for MACS3. Only used when --narrow_peak isnt specified.", "fa_icon": "fas fa-hand-scissors" }, "macs_fdr": { @@ -321,18 +321,18 @@ }, "save_macs_pileup": { "type": "boolean", - "description": "Instruct MACS2 to create bedGraph files normalised to signal per million reads.", + "description": "Instruct MACS3 to create bedGraph files normalised to signal per million reads.", "fa_icon": "fas fa-save" }, "skip_peak_qc": { "type": "boolean", "fa_icon": "fas fa-fast-forward", - "description": "Skip MACS2 peak QC plot generation." + "description": "Skip MACS3 peak QC plot generation." }, "skip_peak_annotation": { "type": "boolean", "fa_icon": "fas fa-fast-forward", - "description": "Skip annotation of MACS2 and consensus peaks with HOMER." + "description": "Skip annotation of MACS3 and consensus peaks with HOMER." }, "skip_consensus_peaks": { "type": "boolean", diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf index 14bac09f..37070807 100644 --- a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -145,7 +145,7 @@ def validateInputParameters() { } if (!params.read_length && !params.macs_gsize) { - error ("Both '--read_length' and '--macs_gsize' not specified! Please specify either to infer MACS2 genome size for peak calling.") + error ("Both '--read_length' and '--macs_gsize' not specified! Please specify either to infer MACS3 genome size for peak calling.") } } @@ -269,6 +269,6 @@ def macsGsizeWarn(log) { log.warn "=============================================================================\n" + " --macs_gsize parameter has not been provided.\n" + " It will be auto-calculated by 'khmer unique-kmers.py' using the '--read_length' parameter.\n" + - " Explicitly provide '--macs_gsize macs2_genome_size' to change this behaviour.\n" + + " Explicitly provide '--macs_gsize macs3_genome_size' to change this behaviour.\n" + "===================================================================================" } diff --git a/test.csv b/test.csv new file mode 100644 index 00000000..c871931f --- /dev/null +++ b/test.csv @@ -0,0 +1,7 @@ +sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate +SPT5_T0,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822153_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822153_2.fastq.gz,1,SPT5,SPT5_INPUT,1 +SPT5_T0,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822154_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822154_2.fastq.gz,2,SPT5,SPT5_INPUT,2 +SPT5_T15,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822157_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822157_2.fastq.gz,1,SPT5,SPT5_INPUT,1 +SPT5_T15,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822158_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822158_2.fastq.gz,2,SPT5,SPT5_INPUT,2 +SPT5_INPUT,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,1,,, +SPT5_INPUT,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,2,,, diff --git a/tower.yml b/tower.yml index 5b1f5f90..5b981d67 100644 --- a/tower.yml +++ b/tower.yml @@ -1,9 +1,9 @@ reports: multiqc_report.html: display: "MultiQC HTML report" - macs2_peak.plots.pdf: - display: "All samples MACS2 peak QC PDF plots" - macs2_annotatePeaks.plots.pdf: + macs3_peak.plots.pdf: + display: "All samples MACS3 peak QC PDF plots" + macs3_annotatePeaks.plots.pdf: display: "All samples HOMER annotatePeaks.pl QC PDF plots" "*.consensus_peaks.plots.pdf": display: "Consensus peaks DESeq2 QC PDF plots" @@ -14,6 +14,6 @@ reports: "*.plotHeatmap.pdf": display: "Per-sample deepTools plotHeatmap PDF plots" "*_peaks.broadPeak": - display: "Per-sample MACS2 broadPeak file" + display: "Per-sample MACS3 broadPeak file" "*_peaks.narrowPeak": - display: "Per-sample MACS2 narrowPeak file" + display: "Per-sample MACS3 narrowPeak file" diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 8f4a9e63..a5a255c8 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -9,9 +9,9 @@ // include { BEDTOOLS_GENOMECOV } from '../modules/local/bedtools_genomecov' include { FRIP_SCORE } from '../modules/local/frip_score' -include { PLOT_MACS2_QC } from '../modules/local/plot_macs2_qc' +include { PLOT_MACS3_QC } from '../modules/local/plot_macs3_qc' include { PLOT_HOMER_ANNOTATEPEAKS } from '../modules/local/plot_homer_annotatepeaks' -include { MACS2_CONSENSUS } from '../modules/local/macs2_consensus' +include { MACS3_CONSENSUS } from '../modules/local/macs3_consensus' include { ANNOTATE_BOOLEAN_PEAKS } from '../modules/local/annotate_boolean_peaks' include { DESEQ2_QC } from '../modules/local/deseq2_qc' include { IGV } from '../modules/local/igv' @@ -50,10 +50,10 @@ include { DEEPTOOLS_PLOTPROFILE } from '../modules/nf-core/deeptools/plo include { DEEPTOOLS_PLOTHEATMAP } from '../modules/nf-core/deeptools/plotheatmap/main' include { DEEPTOOLS_PLOTFINGERPRINT } from '../modules/nf-core/deeptools/plotfingerprint/main' include { KHMER_UNIQUEKMERS } from '../modules/nf-core/khmer/uniquekmers/main' -include { MACS2_CALLPEAK } from '../modules/nf-core/macs2/callpeak/main' +include { MACS3_CALLPEAK } from '../modules/nf-core/macs3/callpeak/main' include { SUBREAD_FEATURECOUNTS } from '../modules/nf-core/subread/featurecounts/main' -include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_MACS2 } from '../modules/nf-core/homer/annotatepeaks/main' +include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_MACS3 } from '../modules/nf-core/homer/annotatepeaks/main' include { HOMER_ANNOTATEPEAKS as HOMER_ANNOTATEPEAKS_CONSENSUS } from '../modules/nf-core/homer/annotatepeaks/main' // @@ -467,29 +467,29 @@ workflow CHIPSEQ { .set { ch_ip_control_bam } // - // MODULE: Call peaks with MACS2 + // MODULE: Call peaks with MACS3 // - MACS2_CALLPEAK ( + MACS3_CALLPEAK ( ch_ip_control_bam, ch_macs_gsize ) - ch_versions = ch_versions.mix(MACS2_CALLPEAK.out.versions.first()) + ch_versions = ch_versions.mix(MACS3_CALLPEAK.out.versions.first()) // - // Filter out samples with 0 MACS2 peaks called + // Filter out samples with 0 MACS3 peaks called // - MACS2_CALLPEAK + MACS3_CALLPEAK .out .peak .filter { meta, peaks -> peaks.size() > 0 } - .set { ch_macs2_peaks } + .set { ch_macs3_peaks } // Create channels: [ meta, ip_bam, peaks ] ch_ip_control_bam - .join(ch_macs2_peaks, by: [0]) + .join(ch_macs3_peaks, by: [0]) .map { it -> [ it[0], it[1], it[3] ] @@ -526,30 +526,30 @@ workflow CHIPSEQ { if (!params.skip_peak_annotation) { // - // MODULE: Annotate peaks with MACS2 + // MODULE: Annotate peaks with MACS3 // - HOMER_ANNOTATEPEAKS_MACS2 ( - ch_macs2_peaks, + HOMER_ANNOTATEPEAKS_MACS3 ( + ch_macs3_peaks, ch_fasta, ch_gtf ) - ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS_MACS2.out.versions.first()) + ch_versions = ch_versions.mix(HOMER_ANNOTATEPEAKS_MACS3.out.versions.first()) if (!params.skip_peak_qc) { // - // MODULE: MACS2 QC plots with R + // MODULE: MACS3 QC plots with R // - PLOT_MACS2_QC ( - ch_macs2_peaks.collect{it[1]}, + PLOT_MACS3_QC ( + ch_macs3_peaks.collect{it[1]}, params.narrow_peak ) - ch_versions = ch_versions.mix(PLOT_MACS2_QC.out.versions) + ch_versions = ch_versions.mix(PLOT_MACS3_QC.out.versions) // // MODULE: Peak annotation QC plots with R // PLOT_HOMER_ANNOTATEPEAKS ( - HOMER_ANNOTATEPEAKS_MACS2.out.txt.collect{it[1]}, + HOMER_ANNOTATEPEAKS_MACS3.out.txt.collect{it[1]}, ch_peak_annotation_header, "_peaks.annotatePeaks.txt" ) @@ -561,14 +561,14 @@ workflow CHIPSEQ { // // Consensus peaks analysis // - ch_macs2_consensus_bed_lib = Channel.empty() - ch_macs2_consensus_txt_lib = Channel.empty() + ch_macs3_consensus_bed_lib = Channel.empty() + ch_macs3_consensus_txt_lib = Channel.empty() ch_deseq2_pca_multiqc = Channel.empty() ch_deseq2_clustering_multiqc = Channel.empty() if (!params.skip_consensus_peaks) { // Create channels: [ meta , [ peaks ] ] // Where meta = [ id:antibody, multiple_groups:true/false, replicates_exist:true/false ] - ch_macs2_peaks + ch_macs3_peaks .map { meta, peak -> [ meta.antibody, meta.id.split('_')[0..-2].join('_'), peak ] @@ -595,20 +595,20 @@ workflow CHIPSEQ { // // MODULE: Generate consensus peaks across samples // - MACS2_CONSENSUS ( + MACS3_CONSENSUS ( ch_antibody_peaks, params.narrow_peak ) - ch_macs2_consensus_bed_lib = MACS2_CONSENSUS.out.bed - ch_macs2_consensus_txt_lib = MACS2_CONSENSUS.out.txt - ch_versions = ch_versions.mix(MACS2_CONSENSUS.out.versions) + ch_macs3_consensus_bed_lib = MACS3_CONSENSUS.out.bed + ch_macs3_consensus_txt_lib = MACS3_CONSENSUS.out.txt + ch_versions = ch_versions.mix(MACS3_CONSENSUS.out.versions) if (!params.skip_peak_annotation) { // // MODULE: Annotate consensus peaks // HOMER_ANNOTATEPEAKS_CONSENSUS ( - MACS2_CONSENSUS.out.bed, + MACS3_CONSENSUS.out.bed, ch_fasta, ch_gtf ) @@ -618,7 +618,7 @@ workflow CHIPSEQ { // MODULE: Add boolean fields to annotated consensus peaks to aid filtering // ANNOTATE_BOOLEAN_PEAKS ( - MACS2_CONSENSUS.out.boolean_txt.join(HOMER_ANNOTATEPEAKS_CONSENSUS.out.txt, by: [0]), + MACS3_CONSENSUS.out.boolean_txt.join(HOMER_ANNOTATEPEAKS_CONSENSUS.out.txt, by: [0]), ) ch_versions = ch_versions.mix(ANNOTATE_BOOLEAN_PEAKS.out.versions) } @@ -633,7 +633,7 @@ workflow CHIPSEQ { .set { ch_antibody_bams } // Create channels: [ meta, [ ip_bams ], saf ] - MACS2_CONSENSUS + MACS3_CONSENSUS .out .saf .map { @@ -679,9 +679,9 @@ workflow CHIPSEQ { params.narrow_peak ? 'narrow_peak' : 'broad_peak', ch_fasta, UCSC_BEDGRAPHTOBIGWIG.out.bigwig.collect{it[1]}.ifEmpty([]), - ch_macs2_peaks.collect{it[1]}.ifEmpty([]), - ch_macs2_consensus_bed_lib.collect{it[1]}.ifEmpty([]), - ch_macs2_consensus_txt_lib.collect{it[1]}.ifEmpty([]) + ch_macs3_peaks.collect{it[1]}.ifEmpty([]), + ch_macs3_consensus_bed_lib.collect{it[1]}.ifEmpty([]), + ch_macs3_consensus_txt_lib.collect{it[1]}.ifEmpty([]) ) ch_versions = ch_versions.mix(IGV.out.versions) } From f8514362914b0c488a5c0574280785549e8d3cc0 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 23 Jul 2024 11:13:43 +0200 Subject: [PATCH 182/201] Update changelog --- CHANGELOG.md | 3 +++ 1 file changed, 3 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index be4590e9..adf84b3d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#385](https://github.com/nf-core/chipseq/issues/385)] - Fix `--save_unaligned` description in schema. - [[PR #392](https://github.com/nf-core/chipseq/pull/392)] - Adding line numbers to warnings/errors messages in `bin/check_samplesheet.py`. - [[#396](https://github.com/nf-core/chipseq/issues/396)] - Check that samplesheet samples IDs do only have alphanumeric characters, dots, dashes or underscores. +- [[#378](https://github.com/nf-core/chipseq/issues/378)] - Switch from macs2 to macs3. ### Software dependencies @@ -35,6 +36,8 @@ Note, since the pipeline is now using Nextflow DSL2, each process will be run wi | Dependency | Old version | New version | | ---------- | ----------- | ----------- | | `chromap` | 0.2.1 | 0.2.4 | +| `macs2` | 2.2.7.1 | | +| `macs3` | | 3.0.1 | | `multiqc` | 1.13 | 1.14 | | `picard` | 2.27.4 | 3.0.0 | | `samtools` | 1.15.1 | 1.17 | From 0191a6fa4d1c3089dd4d79bb7c97379e696db68f Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 23 Jul 2024 11:26:31 +0200 Subject: [PATCH 183/201] Update macs3/callpeak module --- modules.json | 195 ++++++------------------ modules/nf-core/macs3/callpeak/meta.yml | 2 +- 2 files changed, 47 insertions(+), 150 deletions(-) diff --git a/modules.json b/modules.json index a1bfa4c7..e8c2f7aa 100644 --- a/modules.json +++ b/modules.json @@ -8,252 +8,172 @@ "bowtie2/align": { "branch": "master", "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", - "installed_by": [ - "fastq_align_bowtie2", - "modules" - ] + "installed_by": ["fastq_align_bowtie2", "modules"] }, "bowtie2/build": { "branch": "master", "git_sha": "6a24fbe314bb2e6fe6306c29a63076ea87e8eb3c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "bwa/mem": { "branch": "master", "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", - "installed_by": [ - "fastq_align_bwa", - "modules" - ] + "installed_by": ["fastq_align_bwa", "modules"] }, "chromap/chromap": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "fastq_align_chromap", - "modules" - ] + "installed_by": ["fastq_align_chromap", "modules"] }, "chromap/index": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/getchromsizes": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/computematrix": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotfingerprint": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotheatmap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "deeptools/plotprofile": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "gffread": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "homer/annotatepeaks": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "khmer/uniquekmers": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "macs3/callpeak": { "branch": "master", - "git_sha": "6046db922ee6c425a4dff26eeda122dffc3df9c0", - "installed_by": [ - "modules" - ] + "git_sha": "3a3f471ed42f640115b2661edee5b258e6a254c1", + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "phantompeakqualtools": { "branch": "master", "git_sha": "2dfe9afa90fefc70e320140e5f41287f01f324b0", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/collectmultiplemetrics": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "picard/markduplicates": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": [ - "bam_markduplicates_picard", - "modules" - ] + "installed_by": ["bam_markduplicates_picard", "modules"] }, "picard/mergesamfiles": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "preseq/lcextrap": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "samtools/flagstat": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/idxstats": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "samtools/index": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_markduplicates_picard", - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools", "modules"] }, "samtools/sort": { "branch": "master", "git_sha": "ce0b1aed7d504883061e748f492a31bf44c5777c", - "installed_by": [ - "bam_sort_stats_samtools", - "modules" - ] + "installed_by": ["bam_sort_stats_samtools", "modules"] }, "samtools/stats": { "branch": "master", "git_sha": "ec833ac4c29db6005d18baccf3306f557c46b006", - "installed_by": [ - "bam_stats_samtools", - "modules" - ] + "installed_by": ["bam_stats_samtools", "modules"] }, "subread/featurecounts": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "trimgalore": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore", - "modules" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore", "modules"] }, "ucsc/bedgraphtobigwig": { "branch": "master", "git_sha": "911696ea0b62df80e900ef244d7867d177971f73", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "umitools/extract": { "branch": "master", "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", - "installed_by": [ - "fastq_fastqc_umitools_trimgalore" - ] + "installed_by": ["fastq_fastqc_umitools_trimgalore"] }, "untar": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "untarfiles": { "branch": "master", "git_sha": "5c460c5a4736974abde2843294f35307ee2b0e5e", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -262,78 +182,55 @@ "bam_markduplicates_picard": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "bam_sort_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "fastq_align_bowtie2", - "fastq_align_bwa", - "fastq_align_chromap" - ] + "installed_by": ["fastq_align_bowtie2", "fastq_align_bwa", "fastq_align_chromap"] }, "bam_stats_samtools": { "branch": "master", "git_sha": "0c38be7e652a0b2f3a37681ee4c0dbdf85677647", - "installed_by": [ - "bam_markduplicates_picard", - "bam_sort_stats_samtools" - ] + "installed_by": ["bam_markduplicates_picard", "bam_sort_stats_samtools"] }, "fastq_align_bowtie2": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_bwa": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_align_chromap": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "fastq_fastqc_umitools_trimgalore": { "branch": "master", "git_sha": "cfd937a668919d948f6fcbf4218e79de50c2f36f", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "92de218a329bfc9a9033116eb5f65fd270e72ba3", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } } } -} \ No newline at end of file +} diff --git a/modules/nf-core/macs3/callpeak/meta.yml b/modules/nf-core/macs3/callpeak/meta.yml index b1c7cade..1603b8e2 100644 --- a/modules/nf-core/macs3/callpeak/meta.yml +++ b/modules/nf-core/macs3/callpeak/meta.yml @@ -28,7 +28,7 @@ input: - controlbam: type: file description: The control file - - macs2_gsize: + - macs3_gsize: type: string description: | Effective genome size. It can be 1.0e+9 or 1000000000, From b0d81e68c41df260005d880360d07206c7d4972d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 23 Jul 2024 12:02:22 +0200 Subject: [PATCH 184/201] Update subworkflows using macs2 to macs3 after merge --- ...bam_peaks_call_qc_annotate_macs3_homer.nf} | 54 +++++++++---------- ...antify_qc_bedtools_featurecounts_deseq2.nf | 24 ++++----- workflows/chipseq.nf | 26 ++++----- 3 files changed, 52 insertions(+), 52 deletions(-) rename subworkflows/local/{bam_peaks_call_qc_annotate_macs2_homer.nf => bam_peaks_call_qc_annotate_macs3_homer.nf} (76%) diff --git a/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf b/subworkflows/local/bam_peaks_call_qc_annotate_macs3_homer.nf similarity index 76% rename from subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf rename to subworkflows/local/bam_peaks_call_qc_annotate_macs3_homer.nf index 5b7b9b85..69ecb99f 100644 --- a/subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf +++ b/subworkflows/local/bam_peaks_call_qc_annotate_macs3_homer.nf @@ -1,16 +1,16 @@ // -// Call peaks with MACS2, annotate with HOMER and perform downstream QC +// Call peaks with MACS3, annotate with HOMER and perform downstream QC // -include { MACS2_CALLPEAK } from '../../modules/nf-core/macs2/callpeak/main' +include { MACS3_CALLPEAK } from '../../modules/nf-core/macs3/callpeak/main' include { HOMER_ANNOTATEPEAKS } from '../../modules/nf-core/homer/annotatepeaks/main' include { FRIP_SCORE } from '../../modules/local/frip_score' include { MULTIQC_CUSTOM_PEAKS } from '../../modules/local/multiqc_custom_peaks' -include { PLOT_MACS2_QC } from '../../modules/local/plot_macs2_qc' +include { PLOT_MACS3_QC } from '../../modules/local/plot_macs3_qc' include { PLOT_HOMER_ANNOTATEPEAKS } from '../../modules/local/plot_homer_annotatepeaks' -workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { +workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER { take: ch_bam // channel: [ val(meta), [ ip_bam ], [ control_bam ] ] ch_fasta // channel: [ fasta ] @@ -29,29 +29,29 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { ch_versions = Channel.empty() // - // Call peaks with MACS2 + // Call peaks with MACS3 // - MACS2_CALLPEAK ( + MACS3_CALLPEAK ( ch_bam, macs_gsize ) - ch_versions = ch_versions.mix(MACS2_CALLPEAK.out.versions.first()) + ch_versions = ch_versions.mix(MACS3_CALLPEAK.out.versions.first()) // - // Filter out samples with 0 MACS2 peaks called + // Filter out samples with 0 MACS3 peaks called // - MACS2_CALLPEAK + MACS3_CALLPEAK .out .peak .filter { meta, peaks -> peaks.size() > 0 } - .set { ch_macs2_peaks } + .set { ch_macs3_peaks } // Create channels: [ meta, ip_bam, peaks ] ch_bam - .join(ch_macs2_peaks, by: [0]) + .join(ch_macs3_peaks, by: [0]) .map { meta, ip_bam, control_bam, peaks -> [ meta, ip_bam, peaks ] @@ -86,8 +86,8 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { ch_versions = ch_versions.mix(MULTIQC_CUSTOM_PEAKS.out.versions.first()) ch_homer_annotatepeaks = Channel.empty() - ch_plot_macs2_qc_txt = Channel.empty() - ch_plot_macs2_qc_pdf = Channel.empty() + ch_plot_macs3_qc_txt = Channel.empty() + ch_plot_macs3_qc_pdf = Channel.empty() ch_plot_homer_annotatepeaks_txt = Channel.empty() ch_plot_homer_annotatepeaks_pdf = Channel.empty() ch_plot_homer_annotatepeaks_tsv = Channel.empty() @@ -96,7 +96,7 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { // Annotate peaks with HOMER // HOMER_ANNOTATEPEAKS ( - ch_macs2_peaks, + ch_macs3_peaks, ch_fasta, ch_gtf ) @@ -105,15 +105,15 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { if (!skip_peak_qc) { // - // MACS2 QC plots with R + // MACS3 QC plots with R // - PLOT_MACS2_QC ( - ch_macs2_peaks.collect{it[1]}, + PLOT_MACS3_QC ( + ch_macs3_peaks.collect{it[1]}, is_narrow_peak ) - ch_plot_macs2_qc_txt = PLOT_MACS2_QC.out.txt - ch_plot_macs2_qc_pdf = PLOT_MACS2_QC.out.pdf - ch_versions = ch_versions.mix(PLOT_MACS2_QC.out.versions) + ch_plot_macs3_qc_txt = PLOT_MACS3_QC.out.txt + ch_plot_macs3_qc_pdf = PLOT_MACS3_QC.out.pdf + ch_versions = ch_versions.mix(PLOT_MACS3_QC.out.versions) // // Peak annotation QC plots with R @@ -131,11 +131,11 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { } emit: - peaks = ch_macs2_peaks // channel: [ val(meta), [ peaks ] ] - xls = MACS2_CALLPEAK.out.xls // channel: [ val(meta), [ xls ] ] - gapped_peaks = MACS2_CALLPEAK.out.gapped // channel: [ val(meta), [ gapped_peak ] ] - bed = MACS2_CALLPEAK.out.bed // channel: [ val(meta), [ bed ] ] - bedgraph = MACS2_CALLPEAK.out.bdg // channel: [ val(meta), [ bedgraph ] ] + peaks = ch_macs3_peaks // channel: [ val(meta), [ peaks ] ] + xls = MACS3_CALLPEAK.out.xls // channel: [ val(meta), [ xls ] ] + gapped_peaks = MACS3_CALLPEAK.out.gapped // channel: [ val(meta), [ gapped_peak ] ] + bed = MACS3_CALLPEAK.out.bed // channel: [ val(meta), [ bed ] ] + bedgraph = MACS3_CALLPEAK.out.bdg // channel: [ val(meta), [ bedgraph ] ] frip_txt = FRIP_SCORE.out.txt // channel: [ val(meta), [ txt ] ] @@ -144,8 +144,8 @@ workflow BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER { homer_annotatepeaks = ch_homer_annotatepeaks // channel: [ val(meta), [ txt ] ] - plot_macs2_qc_txt = ch_plot_macs2_qc_txt // channel: [ txt ] - plot_macs2_qc_pdf = ch_plot_macs2_qc_pdf // channel: [ pdf ] + plot_macs3_qc_txt = ch_plot_macs3_qc_txt // channel: [ txt ] + plot_macs3_qc_pdf = ch_plot_macs3_qc_pdf // channel: [ pdf ] plot_homer_annotatepeaks_txt = ch_plot_homer_annotatepeaks_txt // channel: [ txt ] plot_homer_annotatepeaks_pdf = ch_plot_homer_annotatepeaks_pdf // channel: [ pdf ] diff --git a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf index 6b9b8efe..294a0eec 100644 --- a/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf +++ b/subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf @@ -5,7 +5,7 @@ include { HOMER_ANNOTATEPEAKS } from '../../modules/nf-core/homer/annotatepeaks/main' include { SUBREAD_FEATURECOUNTS } from '../../modules/nf-core/subread/featurecounts/main' -include { MACS2_CONSENSUS } from '../../modules/local/macs2_consensus' +include { MACS3_CONSENSUS } from '../../modules/local/macs3_consensus' include { ANNOTATE_BOOLEAN_PEAKS } from '../../modules/local/annotate_boolean_peaks' include { DESEQ2_QC } from '../../modules/local/deseq2_qc' @@ -54,18 +54,18 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { // // Generate consensus peaks across samples // - MACS2_CONSENSUS ( + MACS3_CONSENSUS ( ch_antibody_peaks, is_narrow_peak ) - ch_versions = ch_versions.mix(MACS2_CONSENSUS.out.versions) + ch_versions = ch_versions.mix(MACS3_CONSENSUS.out.versions) // // Annotate consensus peaks // if (!skip_peak_annotation) { HOMER_ANNOTATEPEAKS ( - MACS2_CONSENSUS.out.bed, + MACS3_CONSENSUS.out.bed, ch_fasta, ch_gtf ) @@ -75,13 +75,13 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { // MODULE: Add boolean fields to annotated consensus peaks to aid filtering // ANNOTATE_BOOLEAN_PEAKS ( - MACS2_CONSENSUS.out.boolean_txt.join(HOMER_ANNOTATEPEAKS.out.txt, by: [0]), + MACS3_CONSENSUS.out.boolean_txt.join(HOMER_ANNOTATEPEAKS.out.txt, by: [0]), ) ch_versions = ch_versions.mix(ANNOTATE_BOOLEAN_PEAKS.out.versions) } // Create channels: [ meta, [ ip_bams ], saf ] - MACS2_CONSENSUS + MACS3_CONSENSUS .out .saf .map { @@ -134,12 +134,12 @@ workflow BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 { } emit: - consensus_bed = MACS2_CONSENSUS.out.bed // channel: [ bed ] - consensus_saf = MACS2_CONSENSUS.out.saf // channel: [ saf ] - consensus_pdf = MACS2_CONSENSUS.out.pdf // channel: [ pdf ] - consensus_txt = MACS2_CONSENSUS.out.txt // channel: [ pdf ] - consensus_boolean_txt = MACS2_CONSENSUS.out.boolean_txt // channel: [ txt ] - consensus_intersect_txt = MACS2_CONSENSUS.out.intersect_txt // channel: [ txt ] + consensus_bed = MACS3_CONSENSUS.out.bed // channel: [ bed ] + consensus_saf = MACS3_CONSENSUS.out.saf // channel: [ saf ] + consensus_pdf = MACS3_CONSENSUS.out.pdf // channel: [ pdf ] + consensus_txt = MACS3_CONSENSUS.out.txt // channel: [ pdf ] + consensus_boolean_txt = MACS3_CONSENSUS.out.boolean_txt // channel: [ txt ] + consensus_intersect_txt = MACS3_CONSENSUS.out.intersect_txt // channel: [ txt ] featurecounts_txt = SUBREAD_FEATURECOUNTS.out.counts // channel: [ txt ] featurecounts_summary = SUBREAD_FEATURECOUNTS.out.summary // channel: [ txt ] diff --git a/workflows/chipseq.nf b/workflows/chipseq.nf index 4b7fb453..491d5f53 100644 --- a/workflows/chipseq.nf +++ b/workflows/chipseq.nf @@ -22,7 +22,7 @@ include { INPUT_CHECK } from '../subworkflows/local/input_check' include { ALIGN_STAR } from '../subworkflows/local/align_star' include { BAM_FILTER_BAMTOOLS } from '../subworkflows/local/bam_filter_bamtools' include { BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC } from '../subworkflows/local/bam_bedgraph_bigwig_bedtools_ucsc' -include { BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER } from '../subworkflows/local/bam_peaks_call_qc_annotate_macs2_homer.nf' +include { BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER } from '../subworkflows/local/bam_peaks_call_qc_annotate_macs3_homer.nf' include { BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 } from '../subworkflows/local/bed_consensus_quantify_qc_bedtools_featurecounts_deseq2.nf' /* @@ -446,9 +446,9 @@ workflow CHIPSEQ { .set { ch_ip_control_bam } // - // SUBWORKFLOW: Call peaks with MACS2, annotate with HOMER and perform downstream QC + // SUBWORKFLOW: Call peaks with MACS3, annotate with HOMER and perform downstream QC // - BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER ( + BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER ( ch_ip_control_bam, ch_fasta, ch_gtf, @@ -461,7 +461,7 @@ workflow CHIPSEQ { params.skip_peak_annotation, params.skip_peak_qc ) - ch_versions = ch_versions.mix(BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.versions) + ch_versions = ch_versions.mix(BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER.out.versions) // // Consensus peaks analysis @@ -481,7 +481,7 @@ workflow CHIPSEQ { .set { ch_antibody_bams } BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2 ( - BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peaks, + BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER.out.peaks, ch_antibody_bams, ch_fasta, ch_gtf, @@ -491,8 +491,8 @@ workflow CHIPSEQ { params.skip_peak_annotation, params.skip_deseq2_qc ) - ch_macs2_consensus_bed_lib = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.consensus_bed - ch_macs2_consensus_txt_lib = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.consensus_txt + ch_macs3_consensus_bed_lib = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.consensus_bed + ch_macs3_consensus_txt_lib = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.consensus_txt ch_subreadfeaturecounts_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.featurecounts_summary ch_deseq2_pca_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.deseq2_qc_pca_multiqc ch_deseq2_clustering_multiqc = BED_CONSENSUS_QUANTIFY_QC_BEDTOOLS_FEATURECOUNTS_DESEQ2.out.deseq2_qc_dists_multiqc @@ -508,9 +508,9 @@ workflow CHIPSEQ { params.narrow_peak ? 'narrow_peak' : 'broad_peak', ch_fasta, BAM_BEDGRAPH_BIGWIG_BEDTOOLS_UCSC.out.bigwig.collect{it[1]}.ifEmpty([]), - BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peaks.collect{it[1]}.ifEmpty([]), - ch_macs2_consensus_bed_lib.collect{it[1]}.ifEmpty([]), - ch_macs2_consensus_txt_lib.collect{it[1]}.ifEmpty([]) + BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER.out.peaks.collect{it[1]}.ifEmpty([]), + ch_macs3_consensus_bed_lib.collect{it[1]}.ifEmpty([]), + ch_macs3_consensus_txt_lib.collect{it[1]}.ifEmpty([]) ) ch_versions = ch_versions.mix(IGV.out.versions) } @@ -568,9 +568,9 @@ workflow CHIPSEQ { ch_multiqc_phantompeakqualtools_rsc_multiqc.collect{it[1]}.ifEmpty([]), ch_multiqc_phantompeakqualtools_correlation_multiqc.collect{it[1]}.ifEmpty([]), - BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.frip_multiqc.collect{it[1]}.ifEmpty([]), - BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.peak_count_multiqc.collect{it[1]}.ifEmpty([]), - BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER.out.plot_homer_annotatepeaks_tsv.collect().ifEmpty([]), + BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER.out.frip_multiqc.collect{it[1]}.ifEmpty([]), + BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER.out.peak_count_multiqc.collect{it[1]}.ifEmpty([]), + BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER.out.plot_homer_annotatepeaks_tsv.collect().ifEmpty([]), ch_subreadfeaturecounts_multiqc.collect{it[1]}.ifEmpty([]), ch_deseq2_pca_multiqc.collect().ifEmpty([]), From 7238956cb94112d1fcfb9151278eee330f51c203 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 23 Jul 2024 12:58:15 +0200 Subject: [PATCH 185/201] Address review comments --- conf/modules.config | 2 +- test.csv | 7 ------- 2 files changed, 1 insertion(+), 8 deletions(-) delete mode 100644 test.csv diff --git a/conf/modules.config b/conf/modules.config index eb028a67..2251e0ff 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -575,7 +575,7 @@ process { if (!params.skip_peak_annotation) { process { - withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS2_HOMER:HOMER_ANNOTATEPEAKS' { + withName: '.*:BAM_PEAKS_CALL_QC_ANNOTATE_MACS3_HOMER:HOMER_ANNOTATEPEAKS' { ext.args = '-gid' ext.prefix = { "${meta.id}_peaks" } publishDir = [ diff --git a/test.csv b/test.csv deleted file mode 100644 index c871931f..00000000 --- a/test.csv +++ /dev/null @@ -1,7 +0,0 @@ -sample,fastq_1,fastq_2,replicate,antibody,control,control_replicate -SPT5_T0,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822153_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822153_2.fastq.gz,1,SPT5,SPT5_INPUT,1 -SPT5_T0,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822154_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822154_2.fastq.gz,2,SPT5,SPT5_INPUT,2 -SPT5_T15,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822157_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822157_2.fastq.gz,1,SPT5,SPT5_INPUT,1 -SPT5_T15,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822158_1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/atacseq/testdata/SRR1822158_2.fastq.gz,2,SPT5,SPT5_INPUT,2 -SPT5_INPUT,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204809_Spt5-ChIP_Input1_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,1,,, -SPT5_INPUT,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R1.fastq.gz,https://raw.githubusercontent.com/nf-core/test-datasets/chipseq/testdata/SRR5204810_Spt5-ChIP_Input2_SacCer_ChIP-Seq_ss100k_R2.fastq.gz,2,,, From 2b631730a27f1395d192aada2f2d36863e17011d Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Tue, 23 Jul 2024 16:10:33 +0200 Subject: [PATCH 186/201] Pass read group fields to bowtie2 --- CHANGELOG.md | 1 + conf/modules.config | 7 ++++++- subworkflows/local/input_check.nf | 4 ++-- 3 files changed, 9 insertions(+), 3 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index adf84b3d..c33bd644 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -28,6 +28,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[PR #392](https://github.com/nf-core/chipseq/pull/392)] - Adding line numbers to warnings/errors messages in `bin/check_samplesheet.py`. - [[#396](https://github.com/nf-core/chipseq/issues/396)] - Check that samplesheet samples IDs do only have alphanumeric characters, dots, dashes or underscores. - [[#378](https://github.com/nf-core/chipseq/issues/378)] - Switch from macs2 to macs3. +- [[#347](https://github.com/nf-core/chipseq/issues/347)] - Add read group tag to bam files processed by bowtie2. ### Software dependencies diff --git a/conf/modules.config b/conf/modules.config index 2251e0ff..22ae4ec0 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -225,7 +225,12 @@ if (params.aligner == 'bwa') { if (params.aligner == 'bowtie2') { process { withName: 'BOWTIE2_ALIGN' { - ext.args = '' + ext.args = { + [ + meta.read_group ? "--rg-id ${meta.id} --rg SM:${meta.id - ~/_T\d+$/} --rg PL:ILLUMINA --rg LB:${meta.id} --rg PU:1" : '', + params.seq_center ? "--rg CN:${params.seq_center}" : '' + ].join(' ').trim() + } ext.prefix = { "${meta.id}.Lb" } publishDir = [ [ diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 648a2971..40e20bd1 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -29,9 +29,9 @@ def create_fastq_channel(LinkedHashMap row, String seq_center) { meta.antibody = row.antibody meta.control = row.control - def read_group = "\'@RG\\tID:${meta.id}\\tSM:${meta.id.split('_')[0..-2].join('_')}\\tPL:ILLUMINA\\tLB:${meta.id}\\tPU:1\'" + def read_group = "\'@RG\\tID:${meta.id}\\tSM:${meta.id - ~/_T\d+$/}\\tPL:ILLUMINA\\tLB:${meta.id}\\tPU:1\'" if (seq_center) { - read_group = "\'@RG\\tID:${meta.id}\\tSM:${meta.id.split('_')[0..-2].join('_')}\\tPL:ILLUMINA\\tLB:${meta.id}\\tPU:1\\tCN:${seq_center}\'" + read_group = "\'@RG\\tID:${meta.id}\\tSM:${meta.id - ~/_T\d+$/}\\tPL:ILLUMINA\\tLB:${meta.id}\\tPU:1\\tCN:${seq_center}\'" } meta.read_group = read_group From 5682c831e2ea3bd8f74bf40eedf9a7f26255a488 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 16:29:36 +0200 Subject: [PATCH 187/201] Update svg metro map with macs3 --- docs/images/nf-core-chipseq_metro_map_grey.svg | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/docs/images/nf-core-chipseq_metro_map_grey.svg b/docs/images/nf-core-chipseq_metro_map_grey.svg index 7c81c00c..d9db58b2 100644 --- a/docs/images/nf-core-chipseq_metro_map_grey.svg +++ b/docs/images/nf-core-chipseq_metro_map_grey.svg @@ -2446,7 +2446,7 @@ style="font-style:normal;font-variant:normal;font-weight:600;font-stretch:normal;font-size:6.3499999px;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro Semi-Bold';text-align:center;text-anchor:middle;stroke-width:0.26458299" x="375.62692" y="35.110294" - id="tspan2289-4-4">MACS2 + id="tspan2289-4-4">MACS3 Date: Wed, 24 Jul 2024 17:05:54 +0200 Subject: [PATCH 188/201] Update png metro map to change to macs3 --- 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ce528a0e814b9a320d2a1f14ca878d5ce2775ae6 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 17:10:01 +0200 Subject: [PATCH 189/201] Update macs peak count plot --- ...count_plot.png => mqc_macs3_peak_count_plot.png} | Bin 1 file changed, 0 insertions(+), 0 deletions(-) rename docs/images/{mqc_macs2_peak_count_plot.png => mqc_macs3_peak_count_plot.png} (100%) diff --git a/docs/images/mqc_macs2_peak_count_plot.png b/docs/images/mqc_macs3_peak_count_plot.png similarity index 100% rename from docs/images/mqc_macs2_peak_count_plot.png rename to docs/images/mqc_macs3_peak_count_plot.png From 50a2416689b7a03f3a64d7cbad3a6cf15fb46623 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 17:15:29 +0200 Subject: [PATCH 190/201] Update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index c33bd644..7cc0d258 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -29,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - [[#396](https://github.com/nf-core/chipseq/issues/396)] - Check that samplesheet samples IDs do only have alphanumeric characters, dots, dashes or underscores. - [[#378](https://github.com/nf-core/chipseq/issues/378)] - Switch from macs2 to macs3. - [[#347](https://github.com/nf-core/chipseq/issues/347)] - Add read group tag to bam files processed by bowtie2. +- [[PR #406](https://github.com/nf-core/chipseq/pull/406)] - Update metro map to show macs3 instead of macs2. ### Software dependencies From dcd850f3157e276b49425f01a3967cc29362ba86 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 17:35:32 +0200 Subject: [PATCH 191/201] Update svg metro map to show margins --- .../images/nf-core-chipseq_metro_map_grey.svg | 565 +++++++++--------- 1 file changed, 283 insertions(+), 282 deletions(-) diff --git a/docs/images/nf-core-chipseq_metro_map_grey.svg b/docs/images/nf-core-chipseq_metro_map_grey.svg index d9db58b2..4bf901ee 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inkscape:export-xdpi="90" inkscape:export-ydpi="90" /> + style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;baseline-shift:baseline;text-anchor:start;white-space:normal;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:2.53707;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate" /> Sarek  + style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;baseline-shift:baseline;text-anchor:start;white-space:normal;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:2.53707;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate" />  Somatic Sarek  + style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;baseline-shift:baseline;text-anchor:start;white-space:normal;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:2.53707;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate" /> Sarek    Exome + style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:41.4543px;line-height:1.25;font-family:'Eurostile Extended';-inkscape-font-specification:'Eurostile Extended';text-align:start;text-anchor:start">Exome + style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;baseline-shift:baseline;text-anchor:start;white-space:normal;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:2.53707;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate" /> Sarek  + style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;baseline-shift:baseline;text-anchor:start;white-space:normal;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:2.53707;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate" />  Somatic Sarek  + style="color:#000000;font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:medium;line-height:normal;font-family:sans-serif;text-indent:0;text-align:start;text-decoration:none;text-decoration-line:none;text-decoration-style:solid;text-decoration-color:#000000;letter-spacing:normal;word-spacing:normal;text-transform:none;writing-mode:lr-tb;direction:ltr;baseline-shift:baseline;text-anchor:start;white-space:normal;clip-rule:nonzero;display:inline;overflow:visible;visibility:visible;opacity:1;isolation:auto;mix-blend-mode:normal;color-interpolation:sRGB;color-interpolation-filters:linearRGB;solid-color:#000000;solid-opacity:1;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none;stroke-width:2.53707;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-dashoffset:0;stroke-opacity:1;color-rendering:auto;image-rendering:auto;shape-rendering:auto;text-rendering:auto;enable-background:accumulate" /> Sarek    Exome + style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:41.4543px;line-height:1.25;font-family:'Eurostile Extended';-inkscape-font-specification:'Eurostile Extended';text-align:start;text-anchor:start">Exome + y="117.92941" + style="stroke-width:0.264583" /> + y="27.057777" + style="stroke-width:0.264583" /> Pre-processing + style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:5.64444px;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro';stroke:#000000;stroke-width:0.264583;stroke-opacity:1">Pre-processing Genome alignment + style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:5.64444px;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro';stroke:#000000;stroke-width:0.264583;stroke-opacity:1">Genome alignment @@ -1481,86 +1482,86 @@ sodipodi:nodetypes="cc" id="path2500-5-22-5-4-7-4-07" d="m 132.06682,-28.534285 h 94.59215" - style="fill:none;stroke:#ff1465;stroke-width:2.64583325;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1" + style="fill:none;stroke:#ff1465;stroke-width:2.64583;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1" inkscape:connector-curvature="0" /> Cutadapt + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.35px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.264583" /> FastQC FastQC IGV + style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:6.35px;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro';stroke:#000000;stroke-width:0.264583;stroke-opacity:1">IGV phantompeakqualtools MultiQC @@ -1609,29 +1610,29 @@ id="flowPara5862" /> STAR BWA Peak calling & ChIP QC + style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:5.64444px;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro';stroke:#000000;stroke-width:0.264583;stroke-opacity:1">Peak calling & ChIP QC @@ -1728,10 +1729,10 @@ bigWig + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:26.231px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold'">bigWig + style="fill:#ffffff;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill" /> + style="fill:#ffffff;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill" /> + style="fill:#ffffff;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill" /> + style="fill:#ffffff;stroke:#000000;stroke-width:2.64583;stroke-linecap:round;stroke-miterlimit:4;stroke-dasharray:none;paint-order:stroke markers fill" /> Bowtie2 Chromap @@ -1840,59 +1841,59 @@ id="g1967" transform="matrix(0.2434307,0,0,0.2434307,103.90138,113.36827)"> Picard¹ + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.35px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.264583" /> Filtering² Picard + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.35px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.264583" /> Preseq + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.35px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.264583" /> Alignment QC Deeptools BedgraphtoBigWig + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:6.35px;font-family:'DejaVu Sans Mono';-inkscape-font-specification:'DejaVu Sans Mono Bold';text-align:center;text-anchor:middle;stroke-width:0.264583" /> IP enrichment analysis + style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:5.64444px;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro';stroke:#000000;stroke-width:0.264583;stroke-opacity:1">IP enrichment analysis @@ -2353,25 +2354,25 @@ sodipodi:nodetypes="cc" id="path2500-5-22-5-4-7-4-07-7-0-9-2" d="m 132.06682,-28.534285 h 94.59215" - style="fill:none;stroke:#ff1465;stroke-width:2.64583325;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1" + style="fill:none;stroke:#ff1465;stroke-width:2.64583;stroke-linecap:butt;stroke-linejoin:miter;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1" inkscape:connector-curvature="0" /> MACS3 Homer Bedtools FeatureCounts R+DESeq2 XML + style="font-style:normal;font-variant:normal;font-weight:bold;font-stretch:normal;font-size:26.231px;line-height:1.25;font-family:'Liberation Sans';-inkscape-font-specification:'Liberation Sans Bold';text-align:center;direction:rtl;text-anchor:middle">XML @@ -2625,17 +2626,17 @@ + y="69.957184" + style="font-size:5.64444px;stroke:#000000;stroke-width:0.264583;stroke-opacity:1" /> 1. @@ -2669,9 +2670,9 @@ transform="matrix(0.26458333,0,0,0.26458333,-0.15701478,-103.37633)" xml:space="preserve" id="flowRoot2974-6" - style="font-style:normal;font-variant:normal;font-weight:600;font-stretch:normal;font-size:26.66666794px;line-height:1.25;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro Semi-Bold';letter-spacing:0px;word-spacing:0px;fill:#000000;fill-opacity:1;stroke:none">Picard Mark duplicatesMerge alignments from multiple libraries of the same sample + style="font-style:normal;font-variant:normal;font-weight:600;font-stretch:normal;font-size:16px;line-height:0.8;font-family:'Maven Pro';-inkscape-font-specification:'Maven Pro, Semi-Bold';font-variant-ligatures:normal;font-variant-caps:normal;font-variant-numeric:diagonal-fractions;font-feature-settings:normal;text-align:start;writing-mode:lr-tb;direction:ltr;text-anchor:start" /> Pysam (paired-ends only) @@ -2735,17 +2736,17 @@ id="text76489" y="133.17679" x="482.20358" - style="font-size:4.23332977px;line-height:1.54999995;font-family:Commissioner;-inkscape-font-specification:Commissioner;letter-spacing:0px;stroke-width:0.26458299" + style="font-size:4.23333px;line-height:1.55;font-family:Commissioner;-inkscape-font-specification:Commissioner;letter-spacing:0px;stroke-width:0.264583" xml:space="preserve"> License: From fec708eb3b6ae4c24e03b9ab8142914efc5180e5 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 18:22:29 +0200 Subject: [PATCH 192/201] Always upload logs after megatests --- .github/workflows/awsfulltest.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 9c93179c..b40d8bfb 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -33,6 +33,7 @@ jobs: profiles: test_full - uses: actions/upload-artifact@v4 + if: success() || failure() with: name: Seqera Platform debug log file path: | From 9d6919a9d12dfec567cdb1c95285716e66774d40 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 18:30:00 +0200 Subject: [PATCH 193/201] Revert always upload logs after megatests --- .github/workflows/awsfulltest.yml | 1 - 1 file changed, 1 deletion(-) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index b40d8bfb..9c93179c 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -33,7 +33,6 @@ jobs: profiles: test_full - uses: actions/upload-artifact@v4 - if: success() || failure() with: name: Seqera Platform debug log file path: | From cee6b4708c3044918ddc195c36eb1f1f8dcd42e1 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 18:31:29 +0200 Subject: [PATCH 194/201] Always upload logs when running aws full tests --- .github/workflows/awsfulltest.yml | 1 + 1 file changed, 1 insertion(+) diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 9c93179c..b40d8bfb 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -33,6 +33,7 @@ jobs: profiles: test_full - uses: actions/upload-artifact@v4 + if: success() || failure() with: name: Seqera Platform debug log file path: | From fd28fe456b1b888fe28068b2b7bbac05113e3331 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 23:42:07 +0200 Subject: [PATCH 195/201] Read params before including anything --- main.nf | 24 ++++++++++++------------ 1 file changed, 12 insertions(+), 12 deletions(-) diff --git a/main.nf b/main.nf index 32dd637c..63146cd4 100755 --- a/main.nf +++ b/main.nf @@ -11,18 +11,6 @@ nextflow.enable.dsl = 2 -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ -include { CHIPSEQ } from './workflows/chipseq' -include { PREPARE_GENOME } from './subworkflows/local/prepare_genome' -include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' -include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' -include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_chipseq_pipeline' -include { getMacsGsize } from './subworkflows/local/utils_nfcore_chipseq_pipeline' - /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ GENOME PARAMETER VALUES @@ -40,6 +28,18 @@ params.gene_bed = getGenomeAttribute('gene_bed') params.blacklist = getGenomeAttribute('blacklist') params.macs_gsize = getMacsGsize(params) +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ +include { CHIPSEQ } from './workflows/chipseq' +include { PREPARE_GENOME } from './subworkflows/local/prepare_genome' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +include { getMacsGsize } from './subworkflows/local/utils_nfcore_chipseq_pipeline' + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ NAMED WORKFLOWS FOR PIPELINE From b1f7d313b7c638e71aacfc9122673058ee9d9730 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 23:48:45 +0200 Subject: [PATCH 196/201] Move getGenomeAttribute function to main --- main.nf | 19 +++++++++++++++++++ .../utils_nfcore_chipseq_pipeline/main.nf | 16 ++++++++-------- 2 files changed, 27 insertions(+), 8 deletions(-) diff --git a/main.nf b/main.nf index 63146cd4..cdf4f045 100755 --- a/main.nf +++ b/main.nf @@ -137,6 +137,25 @@ workflow { ) } +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + FUNCTIONS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +// +// Get attribute from genome config file e.g. fasta +// + +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf index 37070807..c9f8bb3d 100644 --- a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -152,14 +152,14 @@ def validateInputParameters() { // // Get attribute from genome config file e.g. fasta // -def getGenomeAttribute(attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null -} +// def getGenomeAttribute(attribute) { +// if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { +// if (params.genomes[ params.genome ].containsKey(attribute)) { +// return params.genomes[ params.genome ][ attribute ] +// } +// } +// return null +// } // // Exit pipeline if incorrect --genome key provided From a20c464dae7648e49e2e12cc329955e8f6dc4c1e Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 24 Jul 2024 23:59:04 +0200 Subject: [PATCH 197/201] Also move getMacsGsize declaration --- main.nf | 16 +++++++++++++- .../utils_nfcore_chipseq_pipeline/main.nf | 22 +++++++++---------- 2 files changed, 26 insertions(+), 12 deletions(-) diff --git a/main.nf b/main.nf index cdf4f045..0079ec55 100755 --- a/main.nf +++ b/main.nf @@ -146,7 +146,6 @@ workflow { // // Get attribute from genome config file e.g. fasta // - def getGenomeAttribute(attribute) { if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { if (params.genomes[ params.genome ].containsKey(attribute)) { @@ -156,6 +155,21 @@ def getGenomeAttribute(attribute) { return null } +// +// Get macs genome size (macs_gsize) +// +def getMacsGsize(params) { + def val = null + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey('macs_gsize')) { + if (params.genomes[ params.genome ][ 'macs_gsize' ].containsKey(params.read_length.toString())) { + val = params.genomes[ params.genome ][ 'macs_gsize' ][ params.read_length.toString() ] + } + } + } + return val +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf index c9f8bb3d..dcdaba6c 100644 --- a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -178,17 +178,17 @@ def genomeExistsError() { // // Get macs genome size (macs_gsize) // -def getMacsGsize(params) { - def val = null - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey('macs_gsize')) { - if (params.genomes[ params.genome ][ 'macs_gsize' ].containsKey(params.read_length.toString())) { - val = params.genomes[ params.genome ][ 'macs_gsize' ][ params.read_length.toString() ] - } - } - } - return val -} +// def getMacsGsize(params) { +// def val = null +// if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { +// if (params.genomes[ params.genome ].containsKey('macs_gsize')) { +// if (params.genomes[ params.genome ][ 'macs_gsize' ].containsKey(params.read_length.toString())) { +// val = params.genomes[ params.genome ][ 'macs_gsize' ][ params.read_length.toString() ] +// } +// } +// } +// return val +// } // // Generate methods description for MultiQC From 50caeb9945ef4c41334961dbe1ff981848b0cfbc Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 25 Jul 2024 00:02:35 +0200 Subject: [PATCH 198/201] Remove includes --- main.nf | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/main.nf b/main.nf index 0079ec55..af328320 100755 --- a/main.nf +++ b/main.nf @@ -37,8 +37,8 @@ include { CHIPSEQ } from './workflows/chipseq' include { PREPARE_GENOME } from './subworkflows/local/prepare_genome' include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_chipseq_pipeline' -include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_chipseq_pipeline' -include { getMacsGsize } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +// include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_chipseq_pipeline' +// include { getMacsGsize } from './subworkflows/local/utils_nfcore_chipseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From cd28cd0c92c32da5541e324eab8df8229f1a2e97 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Thu, 25 Jul 2024 10:10:54 +0200 Subject: [PATCH 199/201] Fix output of bwa index when provided as not compressed input --- subworkflows/local/prepare_genome.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index b7878aaf..64350b6a 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -146,7 +146,7 @@ workflow PREPARE_GENOME { ch_bwa_index = UNTAR_BWA_INDEX ( [ [:], bwa_index ] ).untar ch_versions = ch_versions.mix(UNTAR_BWA_INDEX.out.versions) } else { - ch_bwa_index = file(bwa_index) + ch_bwa_index = [ [:], file(bwa_index) ] } } else { ch_bwa_index = BWA_INDEX ( ch_fasta.map { [ [:], it ] } ).index From 588a28af88107d4e74e41384a9c0e340dac8fde6 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 31 Jul 2024 10:00:40 +0200 Subject: [PATCH 200/201] Make lint happy --- subworkflows/local/prepare_genome.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/prepare_genome.nf b/subworkflows/local/prepare_genome.nf index 64350b6a..0d91f377 100644 --- a/subworkflows/local/prepare_genome.nf +++ b/subworkflows/local/prepare_genome.nf @@ -146,7 +146,7 @@ workflow PREPARE_GENOME { ch_bwa_index = UNTAR_BWA_INDEX ( [ [:], bwa_index ] ).untar ch_versions = ch_versions.mix(UNTAR_BWA_INDEX.out.versions) } else { - ch_bwa_index = [ [:], file(bwa_index) ] + ch_bwa_index = [ [:], file(bwa_index) ] } } else { ch_bwa_index = BWA_INDEX ( ch_fasta.map { [ [:], it ] } ).index From 49c405c71356110f32cb33ce9aacc9fa2fd61295 Mon Sep 17 00:00:00 2001 From: JoseEspinosa Date: Wed, 31 Jul 2024 10:59:17 +0200 Subject: [PATCH 201/201] Get rid of the functions in pipeline utils --- .../utils_nfcore_chipseq_pipeline/main.nf | 27 ------------------- 1 file changed, 27 deletions(-) diff --git a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf index dcdaba6c..4ba55198 100644 --- a/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_chipseq_pipeline/main.nf @@ -149,18 +149,6 @@ def validateInputParameters() { } } -// -// Get attribute from genome config file e.g. fasta -// -// def getGenomeAttribute(attribute) { -// if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { -// if (params.genomes[ params.genome ].containsKey(attribute)) { -// return params.genomes[ params.genome ][ attribute ] -// } -// } -// return null -// } - // // Exit pipeline if incorrect --genome key provided // @@ -175,21 +163,6 @@ def genomeExistsError() { } } -// -// Get macs genome size (macs_gsize) -// -// def getMacsGsize(params) { -// def val = null -// if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { -// if (params.genomes[ params.genome ].containsKey('macs_gsize')) { -// if (params.genomes[ params.genome ][ 'macs_gsize' ].containsKey(params.read_length.toString())) { -// val = params.genomes[ params.genome ][ 'macs_gsize' ][ params.read_length.toString() ] -// } -// } -// } -// return val -// } - // // Generate methods description for MultiQC //
    Process Name \\", - " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow

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