diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py
index 36351a71..b7437c12 100755
--- a/bin/check_samplesheet.py
+++ b/bin/check_samplesheet.py
@@ -89,7 +89,7 @@ def check_samplesheet(file_in, file_out):
                 for fastq in [fastq_1, fastq_2]:
                     if fastq:
                         if fastq.find(" ") != -1:
-                            print_error("FastQ file contains spaces!", "Line {}".format(lineNo), line)
+                            print_error("FastQ file contains spaces!", "Line {}".format(line_number), line)
                         if not fastq.endswith(".fastq.gz") and not fastq.endswith(".fq.gz"):
                             print_error(
                                 "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!",