Associated Publication: Johnston et al., 2014
- The maize leaf is an ideal system to study plant morphogenesis
- It is divided into a proximal (close to stem) sheath and a distal blade each with distinct developmental patterning.
- Specialized epidermal fringe ligule and auricle structures form at the blade-sheath boundary.
For full metadata: see the NCBI GEO page for GSE61333
The SRX vs sample relation is given here broadly captured from the full metadata file:
| Sample_relation | SRR_ID | Maize_cultivar | Genotype | Sample_description | Sample_title |
|---|---|---|---|---|---|
| SRX699505 | SRR1573504 | B73 | wild-type | all cell layers Leaf blade; Repeat:3 | B-3 |
| SRX699506 | SRR1573505 | B73 | wild-type | all cell layers Leaf blade; Repeat:4 | B-4 |
| SRX699507 | SRR1573506 | B73 | wild-type | all cell layers Leaf blade; Repeat:5 | B-5 |
| SRX699508 | SRR1573507 | B73 | wild-type | all cell layers Leaf ligule; Repeat:3 | L-3 |
| SRX699509 | SRR1573508 | B73 | wild-type | all cell layers Leaf ligule; Repeat:4 | L-4 |
| SRX699510 | SRR1573509 | B73 | wild-type | all cell layers Leaf ligule; Repeat:5 | L-5 |
| SRX699511 | SRR1573510 | B73 | wild-type | all cell layers Leaf sheath; Repeat:3 | S-3 |
| SRX699512 | SRR1573511 | B73 | wild-type | all cell layers Leaf sheath; Repeat:4 | S-4 |
| SRX699513 | SRR1573512 | B73 | wild-type | all cell layers Leaf sheath; Repeat:5 | S-5 |
| SRX699514 | SRR1573513 | B73 | wild-type | Wild-type tissue from P6 leaf primordia all cell layers. Repeat 1 | wtL-1 |
| SRX699515 | SRR1573514 | B73 | wild-type | Wild-type tissue from P6 leaf primordia all cell layers. Repeat 2 | wtL-2 |
| SRX699516 | SRR1573515 | B73 | wild-type | Wild-type tissue from P6 leaf primordia all cell layers. Repeat 3 | wtL-3 |
| SRX699517 | SRR1573516 | B73 | lg1-R | lg1 mutant tissue from P6 leaf primordia all cell layers. Repeat 1 | lg1-1 |
| SRX699518 | SRR1573517 | B73 | lg1-R | lg1 mutant tissue from P6 leaf primordia all cell layers. Repeat 2 | lg1-2 |
| SRX699519 | SRR1573518 | B73 | lg1-R | lg1 mutant tissue from P6 leaf primordia all cell layers. Repeat 3 | lg1-3 |
| SRX699520 | SRR1573519 | B73 | wild-type | adaxial L1 tissue was captured. blade; Repeat:1 | B_L1.1 |
| SRX699521 | SRR1573520 | B73 | wild-type | adaxial L1 tissue was captured. blade; Repeat:2 | B_L1.2 |
| SRX699522 | SRR1573521 | B73 | wild-type | adaxial L1 tissue was captured. blade; Repeat:3 | B_L1.3 |
| SRX699523 | SRR1573522 | B73 | wild-type | adaxial L1 tissue was captured. ligule; Repeat:1 | L_L1.1 |
| SRX699524 | SRR1573523 | B73 | wild-type | adaxial L1 tissue was captured. ligule; Repeat:2 | L_L1.2 |
| SRX699525 | SRR1573524 | B73 | wild-type | adaxial L1 tissue was captured. ligule; Repeat:3 | L_L1.3 |
| SRX699526 | SRR1573525 | B73 | wild-type | adaxial L1 tissue was captured. sheath; Repeat:1 | S_L1.1 |
| SRX699527 | SRR1573526 | B73 | wild-type | adaxial L1 tissue was captured. sheath; Repeat:2 | S_L1.2 |
| SRX699528 | SRR1573527 | B73 | wild-type | adaxial L1 tissue was captured. sheath; Repeat:3 | S_L1.3 |
For more details refer to the figure below:
This shows how the various tissue samples were microdissected. This is modified from original Fig 2 in Johnston et al. 2014,