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HgRad50.lyx
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#LyX 2.3 created this file. For more info see http://www.lyx.org/
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\begin_body
\begin_layout Chapter
An Extremely Stable Interprotein Tetrahedral Hg(Cys)
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4
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Forms in the Zinc Hook Domain of Rad50 Protein at Physiological pH
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textsubscript{4} Forms in the Zinc Hook Domain of Rad50 Protein at Physiological
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Marek Łuczkowski
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*
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, Michał Padjasek
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*
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, Józef Ba Tran
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*
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, Lars Hemmingsen
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†
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, Olga Kerber
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*
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, Jelena Habjanič
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‡
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, Eva Freisinger
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‡
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and Artur Krężel
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Department of Chemical Biology, Faculty of Biotechnology University of Wrocław,
F.
Joliot-Curie 14a, 50-383 Wrocław, Poland
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†
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Department of Chemistry, University of Copenhagen, Universitetparken 5,
2100 København Ø, Denmark
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‡
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Department of Chemistry, University of Zurich, Winterthurerstrasse, 190,
8057 Zürich, Switzerland
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Chemistry – A European Journal
\emph on
2022 28, e202202738
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\begin_layout Section
Brief history and bibliometric data
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The last two chapters (
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and
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LatexCommand labelonly
reference "chap:AgRad50"
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) presenting the results of my doctoral project focus on the mismetallation
of
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gls{rad}
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from
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gls{pf}
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.
My participation in the first of this studies is quite astonishing, I do
believe that
\begin_inset Quotes eld
\end_inset
Insanity is doing the same thing over and over again, but expecting different
results.
\begin_inset Quotes erd
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A quote wrongly attributed to Albert Einstein.
\end_layout
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, and asking me to perform the same
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gls{itc}
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experiments because the results are not in agreement with the initial hypothesi
s seemed to me as a waste of time, however, for some reason, maybe I had
a hunch, I agreed to perform again the same experiments.
My task was to conduct
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status open
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experiments that would give information about the stability of the Hg(II)–
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gls{rad}
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complex.
The first experiments I have performed gave exactly the same results as
those obtained by my colleagues.
The observed
\begin_inset ERT
status open
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\backslash
gls{dH}
\end_layout
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of binding of Hg(II) to the zinc hook, assuming that the
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status open
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\backslash
gls{dS}
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of binding of Zn(II), Cd(II) and Hg(II) were the same, was too small for
the observed lack of competition between the competitor (e.g., acetylcysteine,
cyanides) and the zinc hook to be explained by the formation constant calculate
d using the measured
\begin_inset ERT
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gls{dH}
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and the
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gls{dS}
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estimated by other experiments (see
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,
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and the rest of the chapter).
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By default, all
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status open
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gls{itc}
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experiments, I and my predecessors have performed, were performed in buffers
containing
\begin_inset ERT
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ce{Cl-}
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from
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ce{NaCl}
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intended to provide ionic strength.
In the case of other measurements made with Zn(II),
\begin_inset CommandInset citation
LatexCommand citep
key "Kochanczyk2016a"
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or Cd(II),
\begin_inset CommandInset citation
LatexCommand citep
key "Padjasek2020a"
literal "true"
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this did not make any difference, but in this case it did.
Browsing the literature in search of constants of formation of mercury
complexes with various compounds, I came across information on enthalpies
of formation of Hg(II) complexes with chlorides,
\emph on
eureka!
\emph default
, I have calculated the chemical speciation of Hg(II) and
\begin_inset ERT
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ce{Cl-}
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complexes, and along with the speciation I have calculated the sum of enthalpie
s of formation of the complexes.
According to Hess's law, the enthalpy of formation of the Hg(
\begin_inset ERT
status open
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\backslash
gls{rad}
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)
\begin_inset script subscript
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2
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complex should be diminished by the enthalpy of formation of various Hg(II)
complexes with chlorides.
One more I have repeated the
\begin_inset ERT
status open
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gls{itc}
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experiments using a different mercury salt and a different buffer (
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status open
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ce{Cl-}
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-free), unsurprisingly the difference in the observed enthalpy was consistent
with my theoretical calculations.
The measured enthalpy allowed me to
\emph on
estimate
\emph default
the formation constant of the Hg(II) and
\begin_inset ERT
status open
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\backslash
gls{rad}
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complex.
What was very nice, was the fact that the reviewers assigned by
\shape italic
Chemistry – A European Journal
\emph on
(
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\emph default
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status open
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gls{if}
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of 5.020
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\emph on
, March 6, 2023) have pointed out an interesting application of
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status open
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gls{itc}
\end_layout
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for measuring the complex stability.
\shape default
\emph default
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status open
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\backslash
begin{pycode}[sessionA]
\end_layout
\begin_layout Plain Layout
from scholarly import scholarly
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num2words = {1: 'once',
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2: 'two',
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30: 'thirty',
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40: 'forty',
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50: 'fifty',
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60: 'sixty',
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70: 'seventy',
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90: 'ninety',
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0: 'zero'}
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def n2w(n):
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try:
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return (num2words[n])
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except KeyError:
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try:
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return ((num2words[n-n % 10]) + ' ' + (num2words[n % 10].lower()))
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return ('Number out of range')
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\begin_layout Plain Layout
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\begin_layout Plain Layout
search_query = scholarly.search_single_pub(
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'An Extremely Stable Interprotein Tetrahedral Hg(Cys)4 Forms in the
Zinc Hook Domain of Rad50 Protein at Physiological pH')
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num_citations = search_query['num_citations']
\end_layout
\begin_layout Plain Layout
\end_layout
\begin_layout Plain Layout
if num_citations <= 10:
\end_layout
\begin_layout Plain Layout
if num_citations == 1:
\end_layout