Error in access(object, "otu_table", errorIfNULL) :

[kdbchau]

I'm trying to rarefy my data, and I want to first remove samples with less than 500 reads but I am running into an issue.

My phyloseq object:

phyloseq-class experiment-level object
otu_table()   OTU Table:         [ 457 taxa and 88 samples ]
sample_data() Sample Data:       [ 88 samples by 4 sample variables ]
tax_table()   Taxonomy Table:    [ 457 taxa by 6 taxonomic ranks ]

The code and error:

# Remove samples with < 500 reads total
samples_over500 <- subset_samples(phylo, sample_sums(phylo) > 500)

Error in access(object, "otu_table", errorIfNULL) : 
  otu_table slot is empty.

I've checked other sources for this error and there doesn't seem to be a clear reason why this occurs. I am using Phyloseq package version 1.42.0 on R Studio version 2024.04.2, with R version 4.2.0