From 167fec50060316fc4cd280d7bc319e14a63bb553 Mon Sep 17 00:00:00 2001 From: Ira Iosub Date: Wed, 13 Dec 2023 20:39:10 +0000 Subject: [PATCH] updated run instructions --- README.md | 8 ++++---- modules/local/ribowaltz.nf | 2 +- run.sh | 6 +++--- 3 files changed, 8 insertions(+), 8 deletions(-) diff --git a/README.md b/README.md index 185f1c0..1783232 100644 --- a/README.md +++ b/README.md @@ -49,7 +49,7 @@ We recommend using Nextflow with `Java 17.0.9` or later. 2. Pull the desired version of the pipeline from the GitHub repository: ``` -nextflow pull iraiosub/riboseq-flow -r v1.0.0 +nextflow pull iraiosub/riboseq-flow -r v1.0.1 ``` 3. Run the pipeline on the provided test dataset: @@ -57,13 +57,13 @@ nextflow pull iraiosub/riboseq-flow -r v1.0.0 Using Singularity: ``` -nextflow run iraiosub/riboseq-flow -r v1.0.0 -profile test,singularity +nextflow run iraiosub/riboseq-flow -r v1.0.1 -profile test,singularity ``` or using Docker: ``` -nextflow run iraiosub/riboseq-flow -r v1.0.0 -profile test,docker +nextflow run iraiosub/riboseq-flow -r v1.0.1 -profile test,docker ``` 4. Check succesful execution. @@ -75,7 +75,7 @@ nextflow run iraiosub/riboseq-flow -r v1.0.0 -profile test,docker 2. Pull the desired version of the pipeline from the GitHub repository: ``` -nextflow pull iraiosub/riboseq-flow -r v1.0.0 +nextflow pull iraiosub/riboseq-flow -r v1.0.1 ``` 3. Create a samplesheet `samplesheet.csv` with information about the samples you would like to analyse before running the pipeline. It has to be a comma-separated file with 2 columns, and a header row as shown in the example below. diff --git a/modules/local/ribowaltz.nf b/modules/local/ribowaltz.nf index a4d071e..5c2ff64 100644 --- a/modules/local/ribowaltz.nf +++ b/modules/local/ribowaltz.nf @@ -66,7 +66,7 @@ process GET_PSITE_TRACKS { path(fai) output: - path("*.psites.bed"), emit: psite_bed + path("*.psites.bed.gz"), emit: psite_bed // path("*.bigwig"), emit: psite_bigwig script: diff --git a/run.sh b/run.sh index e899d44..23b0926 100755 --- a/run.sh +++ b/run.sh @@ -1,5 +1,5 @@ #!/usr/bin/sh -#SBATCH --job-name=nf-ribo-seq-test +#SBATCH --job-name=riboseq-flow-test #SBATCH --ntasks=1 #SBATCH --time=24:00:00 #SBATCH --mem-per-cpu=4G @@ -17,7 +17,7 @@ ml Singularity/3.6.4 export NXF_SINGULARITY_CACHEDIR=/nemo/lab/ulej/home/shared/singularity export NXF_HOME=/nemo/lab/ulej/home/users/luscomben/users/iosubi/.nextflow -nextflow pull iraiosub/riboseq-flow -r main +nextflow pull iraiosub/riboseq-flow -r v1.0.1 -nextflow run iraiosub/riboseq-flow -r main \ +nextflow run iraiosub/riboseq-flow -r v1.0.1 \ -profile test,singularity \ No newline at end of file