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updated run instructions
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Ira Iosub committed Dec 13, 2023
1 parent 8d826cd commit 167fec5
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8 changes: 4 additions & 4 deletions README.md
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Expand Up @@ -49,21 +49,21 @@ We recommend using Nextflow with `Java 17.0.9` or later.
2. Pull the desired version of the pipeline from the GitHub repository:

```
nextflow pull iraiosub/riboseq-flow -r v1.0.0
nextflow pull iraiosub/riboseq-flow -r v1.0.1
```

3. Run the pipeline on the provided test dataset:

Using Singularity:

```
nextflow run iraiosub/riboseq-flow -r v1.0.0 -profile test,singularity
nextflow run iraiosub/riboseq-flow -r v1.0.1 -profile test,singularity
```

or using Docker:

```
nextflow run iraiosub/riboseq-flow -r v1.0.0 -profile test,docker
nextflow run iraiosub/riboseq-flow -r v1.0.1 -profile test,docker
```

4. Check succesful execution.
Expand All @@ -75,7 +75,7 @@ nextflow run iraiosub/riboseq-flow -r v1.0.0 -profile test,docker
2. Pull the desired version of the pipeline from the GitHub repository:

```
nextflow pull iraiosub/riboseq-flow -r v1.0.0
nextflow pull iraiosub/riboseq-flow -r v1.0.1
```

3. Create a samplesheet `samplesheet.csv` with information about the samples you would like to analyse before running the pipeline. It has to be a comma-separated file with 2 columns, and a header row as shown in the example below.
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2 changes: 1 addition & 1 deletion modules/local/ribowaltz.nf
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Expand Up @@ -66,7 +66,7 @@ process GET_PSITE_TRACKS {
path(fai)

output:
path("*.psites.bed"), emit: psite_bed
path("*.psites.bed.gz"), emit: psite_bed
// path("*.bigwig"), emit: psite_bigwig

script:
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6 changes: 3 additions & 3 deletions run.sh
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@@ -1,5 +1,5 @@
#!/usr/bin/sh
#SBATCH --job-name=nf-ribo-seq-test
#SBATCH --job-name=riboseq-flow-test
#SBATCH --ntasks=1
#SBATCH --time=24:00:00
#SBATCH --mem-per-cpu=4G
Expand All @@ -17,7 +17,7 @@ ml Singularity/3.6.4
export NXF_SINGULARITY_CACHEDIR=/nemo/lab/ulej/home/shared/singularity
export NXF_HOME=/nemo/lab/ulej/home/users/luscomben/users/iosubi/.nextflow

nextflow pull iraiosub/riboseq-flow -r main
nextflow pull iraiosub/riboseq-flow -r v1.0.1

nextflow run iraiosub/riboseq-flow -r main \
nextflow run iraiosub/riboseq-flow -r v1.0.1 \
-profile test,singularity

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