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ckmah committed Mar 21, 2018
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10 changes: 5 additions & 5 deletions Single-Cell RNA-seq Clustering Analysis Notebook.ipynb
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"\n",
"### Analysis Overview\n",
"\n",
"1. <a href=\"#Step-1:-Setup-Analysis\">**Setup Analysis**</a>\n",
"1. [**Setup Analysis**](#Step-1:-Setup-Analysis)\n",
" 1. Load raw count matrix.\n",
"2. <a href=\"#Step-2:-Preprocess-Counts\">**Preprocess Counts**</a>\n",
"2. [**Preprocess Counts**](#Step-2:-Preprocess-Counts)\n",
" 1. Filter cells based on QC metrics.\n",
" 2. Perform data normalization and scaling.\n",
" 3. Remove unwanted sources of variation (number of detected molecules per cell as well as the percentage mitochondrial gene content).\n",
" 4. Detect highly variable genes.\n",
" 5. Perform linear dimensional reduction (PCA).\n",
"3. <a href=\"#Step-3:-Cluster-Cells\">**Cluster Cells**</a>\n",
"3. [**Cluster Cells**](#Step-3:-Cluster-Cells)\n",
" 1. Cluster cells (graph-based clustering) in PCA space and visualize using t-SNE.\n",
"4. <a href=\"#Step-4:-Visualize-Cluster-Markers\">**Visualize Cluster Markers**</a>\n",
"4. [**Visualize Cluster Markers**](#Step-4:-Visualize-Cluster-Markers)\n",
" 1. Explore and visualize cluster markers interactively.\n",
"5. <a href=\"#Step-5:-Export-Analysis-Data\">**Export Analysis Data**</a>\n",
"5. [**Export Analysis Data**](#Step-5:-Export-Analysis-Data)\n",
" 1. Export data to `.csv` files or a compressed `.h5ad` format."
]
},
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