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identificacao.html
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<!DOCTYPE html>
<html lang="prt-br"> <!-- português -->
<head>
<meta charset="utf-8"> <!-- caracteres especiais -->
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> <!-- Permite usar o plug-in do Google Chrome que tem um mecanismo rápido de JavaScript ao abrir páginas no navegador -->
<title>FluxPRT</title> <!-- nome do Guia Interativo FluxPRT -->
<meta name="description" content="">
<meta name="viewport" content="width=device-width, initial-scale=1.0"> <!-- permite controlar a janela de visualização, reduzindo a página web inteira para caber na tela de celulares e ipads-->
<link rel="apple-touch-icon" href="apple-touch-icon.png"> <!-- Adapta os icones para vários dispositivos da apple-->
<!-- arquivos CSS -->
<link rel="stylesheet" href="css/bootstrap.min.css">
<link rel="stylesheet" href="css/bootstrap-theme.min.css">
<link rel="stylesheet" href="css/fontAwesome.css">
<link rel="stylesheet" href="css/light-box.css">
<link rel="stylesheet" href="css/owl-carousel.css">
<link rel="stylesheet" href="css/templatemo-style.css">
<link href="https://fonts.googleapis.com/css?family=Open+Sans:300,400,600,700,800" rel="stylesheet"> <!-- chamar a familia da fonte OpenSans -->
<script src="js/vendor/modernizr-2.8.3-respond-1.4.2.min.js"></script> <!-- biblioteca de js que detecta os recursos disponíveis no navegador e informa quando o navegador não é compatível -->
</head>
<body>
<header class="nav-down responsive-nav hidden-lg hidden-md">
<button type="button" id="nav-toggle" class="navbar-toggle" data-toggle="collapse" data-target="#main-nav">
<span class="sr-only">Toggle navigation</span> <!-- têcnica usada para acessibilidade-->
<span class="icon-bar"></span>
<span class="icon-bar"></span>
<span class="icon-bar"></span>
</button>
<!-- cabecalho da barra de navegação /navbar-header/ -->
<div id="cabecalho da barra de navegação" class="collapse navbar-collapse"><nav><ul class="nav navbar-nav">
<li><a href="#top">Estrutura</a></li>
<li><a href="#video">Apresentação</a></li>
<li><a href="#blog">Guia</a></li>
<li><a href="#contact">Contato</a></li>
</ul></nav>
</div>
</header>
<div class="sidebar-navigation hidde-sm hidden-xs">
<div class="logo">
<a href="index.html"><em>Flux</em>PRT</a>
</div>
<nav>
<ul>
<li>
<a href="#video">
<em>Identificação</em>
</a>
</li>
<li>
<a href="#video">
<span class="rect"></span>
<span class="circle"></span>
Fluxo de trabalho
</a>
</li>
</li>
<li>
<a href="#blog">
<span class="rect"></span>
<span class="circle"></span>
Probabilidade de erro posterior
</a>
</li>
<li>
<a href="#contact">
<span class="rect"></span>
<span class="circle"></span>
e-book
</a>
</li>
</ul>
</nav>
<ul class="social-icons">
<li><a href="index.html"><i class="fa fa-home"></i></a></li> <!-- gitlab do LUAR -->
<li><a href="http://cabernet.luar.dcc.ufmg.br:7070/groups/luar" target="_blank"><i class="fa fa-gitlab"></i></a></li> <!-- gitlab do LUAR -->
<li><a href="http://luar.dcc.ufmg.br/" target="_blank"><i class="fa fa-share-alt"></i></a></li> <!-- webpage do LUAR -->
<li><a href="mailto:elizabeth.espinoza@ufv.br" target="_blank"><i class="fa fa-envelope"></i></a></li> <!-- email do desenvolvedor -->
</ul>
</div>
<div class="page-content">
<section id="video" class="content-section">
<div class="row"><div class="col-md-12"><div class="section-heading">
<h1>Você está na atividade <em>Identificação</em> do FluxPRT</h1>
<p>O Guia Interativo do FluxPRT segue o Fluxo de Trabalho em Proteômica.</p>
</div>
<div class="text-content">
<p>O Guia Interativo do FluxPRT segue o Fluxo de Trabalho em Proteômica apresentado a seguir, clique nas atividades enumeradas mais detalhes e dicas. Aproveite instruções e dicas para tornar sua experiência com o FluxPRT ainda mais produtiva, nesta seção sao apresentadas informacões a respeito da Identificação.<br><br></p>
</div>
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</section>
<section id="blog" class="content-section">
<div class="section-heading">
<h1><em>Probabilidade</em><br>de erro posterior</h1><p>É a probabilidade de um peptídeo ser um golpe falso.<br>Conceitos importantes.</p>
</div>
<div class="tabgroup">
<h4>Fundamentos e técnicas</h4>
<p>Na a identificação de peptídeos, proteínas e sites de Modificações Pós-Traducionais (PTM) se usa o conceito de Probabilidade de erro posterior (<em>posterior error probability</em>, PEP) para integraras múltiplas propriedades peptídeas, como o comprimento, a carga e o número de modificações, pontuando-as em uma única quantidade (Tyanova, Temu, and Cox, 2016), o que reflete na qualidadede um Espectro Compatível de Peptídeos (<em>peptide spectrum match</em>, PSM).</p>
<p>O PEP é a probabilidade de que o PSM observado esteja incorreto, ou seja, a probabilidade de um peptídeo ser um golpe falso (Käll, Storey, MacCoss, and Noble, 2008), esta probabilidade condicional é calculada usando o teorema de Bayes e depende de fatores como: a pontuação de Andrômeda, o comprimento do peptídeo, o número de modificações variáveis, o estado de carga e o número de clivagens perdidas (Tyanova, Temu, and Cox, 2016; Yi, Gong, and Fu, 2020).</p>
</div>
<br><br><strong>Referências</strong><br><br>
<p>Stefka Tyanova, Tikira Temu, and Juergen Cox. <a href="https://doi.org/nprot.2016.136" target="_blank">The maxquant computational platform for mass spectrometry-based shotgun proteomics</a>. <em>Nature protocols</em>, 11(12):2301–2319, 2016. ISSN1750-2799. doi: 10.1038/nprot.2016.136.</p>
<p>Lukas Käll, John D Storey, Michael J MacCoss, and William Stafford Noble. <a href="https://doi.org/10.1021/pr700739d" target="_blank">Posterior error probabilities and false discovery rates: two sides of the same coin</a>. <em>Journal of Proteome Research</em>,7(01):40–44, 2008. doi: 10.1021/pr700739d.</p>
<p>Xinpei Yi, Fuzhou Gong, and Yan Fu. <a href="https://link.springer.com/content/pdf/10.1186/s12859-020-3485-y.pdf" target="_blank">Transfer posterior error probability estimation for peptide identification</a>. <em>BMC Bioinformatics</em>, 21:1–17, 2020.ISSN 1471-2105. doi: 10.1186/s12859-020-3485-y.</p>
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