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the produced bam files do not have the canonical format: this is the one that has been produced:
samtools view -h /mnt/Z/Shared\ folder/Shared\ data/DNA/NGS_AP01_cfrezza_A006200317/A006200317_201074_S18_L000/A006200317_201074_S18_L000.bam | head -50 @HD VN:1.5 SO:unsorted GO:query @SQ SN:Chromosome LN:4686137 @PG ID:bowtie2 PN:bowtie2 VN:2.5.1 CL:"mambaforge/envs/cutruntools2.1/bin/bowtie2-align-s --wrapper basic-0 -p 8 --dovetail --phred33 -x CUT-RUNTools-2.0/assemblies/EB1/Sequence/Bowtie2Index/genome -1 /mnt/c/AP01/A006200317_201074_S18_L000/trimmed2/A006200317_201074_S18_L000_1.paired.fastq.gz -2 /mnt/c/AP01/A006200317_201074_S18_L000/trimmed2/A006200317_201074_S18_L000_2.paired.fastq.gz" @PG ID:samtools PN:samtools PP:bowtie2 VN:1.15.1 CL:mambaforge/envs/cutruntools2.1/bin/samtools view -bS - @PG ID:samtools.1 PN:samtools PP:samtools VN:1.15.1 CL:samtools view -h /mnt/Z/Shared folder/Shared data/DNA/NGS_AP01_cfrezza_A006200317/A006200317_201074_S18_L000/A006200317_201074_S18_L000.bam
and this is the the expected one:
@HD VN:1.5 SO:coordinate @SQ SN:chr1 LN:195471971 @SQ SN:chr2 LN:182113224 @SQ SN:chr3 LN:160039680 @SQ SN:chr4 LN:156508116 @SQ SN:chr5 LN:151834684 @SQ SN:chr6 LN:149736546 @SQ SN:chr7 LN:145441459 @SQ SN:chr8 LN:129401213 @SQ SN:chr9 LN:124595110 @SQ SN:chr10 LN:130694993 @SQ SN:chr11 LN:122082543 @SQ SN:chr12 LN:120129022 @SQ SN:chr13 LN:120421639 @SQ SN:chr14 LN:124902244 @SQ SN:chr15 LN:104043685 @SQ SN:chr16 LN:98207768 @SQ SN:chr17 LN:94987271 @SQ SN:chr18 LN:90702639 @SQ SN:chr19 LN:61431566 @SQ SN:chrX LN:171031299 @SQ SN:chrY LN:91744698 @SQ SN:chrM LN:16299 @SQ SN:GL456210.1 LN:169725 @SQ SN:GL456211.1 LN:241735 @SQ SN:GL456212.1 LN:153618 @SQ SN:GL456213.1 LN:39340 @SQ SN:GL456216.1 LN:66673 @SQ SN:GL456219.1 LN:175968 @SQ SN:GL456221.1 LN:206961 @SQ SN:GL456233.1 LN:336933 @SQ SN:GL456239.1 LN:40056 @SQ SN:GL456350.1 LN:227966 @SQ SN:GL456354.1 LN:195993 @SQ SN:GL456359.1 LN:22974 @SQ SN:GL456360.1 LN:31704 @SQ SN:GL456366.1 LN:47073 @SQ SN:GL456367.1 LN:42057 @SQ SN:GL456368.1 LN:20208 @SQ SN:GL456370.1 LN:26764 @SQ SN:GL456372.1 LN:28664 @SQ SN:GL456378.1 LN:31602 @SQ SN:GL456379.1 LN:72385 @SQ SN:GL456381.1 LN:25871 @SQ SN:GL456382.1 LN:23158 @SQ SN:GL456383.1 LN:38659 @SQ SN:GL456385.1 LN:35240 @SQ SN:GL456387.1 LN:24685 @SQ SN:GL456389.1 LN:28772 @SQ SN:GL456390.1 LN:24668 @SQ SN:GL456392.1 LN:23629 @SQ SN:GL456393.1 LN:55711 @SQ SN:GL456394.1 LN:24323 @SQ SN:GL456396.1 LN:21240 @SQ SN:JH584292.1 LN:14945 @SQ SN:JH584293.1 LN:207968 @SQ SN:JH584294.1 LN:191905 @SQ SN:JH584295.1 LN:1976 @SQ SN:JH584296.1 LN:199368 @SQ SN:JH584297.1 LN:205776 @SQ SN:JH584298.1 LN:184189 @SQ SN:JH584299.1 LN:953012 @SQ SN:JH584300.1 LN:182347 @SQ SN:JH584301.1 LN:259875 @SQ SN:JH584302.1 LN:155838 @SQ SN:JH584303.1 LN:158099 @SQ SN:JH584304.1 LN:114452 @PG ID:bwa PN:bwa VN:0.7.17-r1198-dirty CL:./bwa mem -t 8 /mnt/c/references/GRCm38_gencode19/BWAIndex/genome.fa /mnt/c/AP01/A006200317_201074_S18_L000_R1_001.fastq.gz /mnt/c/AP01/A006200317_201074_S18_L000_R2_001.fastq.gz @PG ID:samtools PN:samtools PP:bwa VN:1.13 CL:samtools sort -@ 8 -o /mnt/c/AP01/A006200317_201074.bam - @PG ID:samtools.1 PN:samtools PP:samtools VN:1.15.1 CL:samtools view -H /mnt/c/AP01/bam2/A006200317_201074.bam
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the produced bam files do not have the canonical format:
this is the one that has been produced:
and this is the the expected one:
The text was updated successfully, but these errors were encountered: