diff --git a/conformance_tests.yaml b/conformance_tests.yaml
index cc2cb1e..28b8be1 100644
--- a/conformance_tests.yaml
+++ b/conformance_tests.yaml
@@ -3258,3 +3258,24 @@
     hash-prefixed "main"
   tags: [ required, command_line_tool ]
 
+- job: tests/itemseparator-job1.yml
+  output:
+    o:
+      class: File
+      checksum: sha1$1f44fbdb98150d4ba212e7b2cdec6c9bda27f6ef
+      size: 8
+  tool: tests/itemseparator1.cwl
+  label: itemseparator_multiple_items
+  doc: Test itemSeparator with multiple inputs in array input.
+  tags: [ required, command_line_tool ]
+
+- job: tests/itemseparator-job2.yml
+  output:
+    o:
+      class: File
+      checksum: sha1$90ce62edf2fe4940e041a68b13e7b5f9d02bbf51
+      size: 4
+  tool: tests/itemseparator1.cwl
+  label: itemseparator_single_item
+  doc: Test itemSeparator with single input in array input.
+  tags: [ required, command_line_tool ]
diff --git a/tests/itemseparator-job1.yml b/tests/itemseparator-job1.yml
new file mode 100644
index 0000000..1eb2d19
--- /dev/null
+++ b/tests/itemseparator-job1.yml
@@ -0,0 +1,2 @@
+i:
+  ['b', 'd']
\ No newline at end of file
diff --git a/tests/itemseparator-job2.yml b/tests/itemseparator-job2.yml
new file mode 100644
index 0000000..e6a4eee
--- /dev/null
+++ b/tests/itemseparator-job2.yml
@@ -0,0 +1,2 @@
+i:
+  ['b']
\ No newline at end of file
diff --git a/tests/itemseparator1.cwl b/tests/itemseparator1.cwl
new file mode 100644
index 0000000..70c770b
--- /dev/null
+++ b/tests/itemseparator1.cwl
@@ -0,0 +1,24 @@
+#!/usr/bin/env cwl-runner
+class: CommandLineTool
+cwlVersion: v1.1.0-dev1
+doc: "Test itemSeparator with prefix"
+hints:
+  ResourceRequirement:
+    ramMin: 8
+    coresMax: 1
+inputs:
+  i:
+    type: string[]
+    label: Input File
+    doc: "Echo these strings"
+    inputBinding: 
+      position: 1
+      prefix: a
+      itemSeparator: " c "
+outputs:
+  o:
+    type: File
+    outputBinding:
+      glob: output.txt
+baseCommand: echo
+stdout: output.txt