This colab notebook and the associated data have been given as a workshop on genome sequencing and long read data. The notebook is accessible to non-coders and introductory biologists. They can also work as a self-guided tutorial on using nanopore sequencing data for plant biology/pathology research. This repository includes step by step base calling of Arabidopsis NLR .fast5 files and alignment to the gene of interest. To get started, open the .ipynb file in Google Colab and click along! ✨
The background slides used in this workshop provide an overview of the history of sequencing and nanopore basecalling and are included as .pdf and .pptx files.