From 7f33d210dadf77c044f8f36e05ff7da0d9a00e3a Mon Sep 17 00:00:00 2001 From: sdentro Date: Sat, 17 Nov 2018 17:51:13 +0000 Subject: [PATCH 01/32] fix installation of copynumber dependency of brass --- build/rlib-build.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build/rlib-build.R b/build/rlib-build.R index dddaab1..d12986f 100644 --- a/build/rlib-build.R +++ b/build/rlib-build.R @@ -36,4 +36,4 @@ ipak(c("ggplot2")) ipak(c("gridExtra")) ipak(c("gtools")) -install_github("sb43/copynumber", ref="f1688edc154f1a0e3aacf7781090afe02882f623") +install_github("Irrationone/copynumber", ref="87d2663fe6b11c03cf6006b4ee9ed70450eacb5a") From 7998c6d112279735673842dd452cd8ed6d3f072d Mon Sep 17 00:00:00 2001 From: sdentro Date: Sat, 17 Nov 2018 21:52:00 +0000 Subject: [PATCH 02/32] Fix issue where incorrect timing file was created when skipping qc --- scripts/analysisWGS.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 51c264b..73e2492 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -446,7 +446,7 @@ do_parallel[CaVEMan_annot]="AnnotateVcf.pl -t -c $REF_BASE/vagrent/vagrent.cache -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman/${NAME_MT}_vs_${NAME_WT}.annot.muts.vcf" if [ ! -z ${SKIPQC+x} ]; then - do_parallel[verify_WT]="echo 'VerifyBam Tumour disabled by params'" + do_parallel[verify_MT]="echo 'VerifyBam Tumour disabled by params'" else echo -e "\t[Parallel block 6] VerifyBam Tumour added..." do_parallel[verify_MT]="verifyBamHomChk.pl -d 25 \ From 8eadd6e106b7764a277ea260db75ea6e32efffb1 Mon Sep 17 00:00:00 2001 From: sdentro Date: Sun, 18 Nov 2018 13:43:09 +0000 Subject: [PATCH 03/32] Fixed crash in tarballing step when no QC output has been generated --- scripts/analysisWGS.sh | 6 +++++- 1 file changed, 5 insertions(+), 1 deletion(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 73e2492..32a1715 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -484,7 +484,11 @@ fi echo 'Package results' # timings first tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.timings.tar.gz timings -tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT} ${PROTOCOL}_${NAME_MT} ${PROTOCOL}_${NAME_WT} +if [ ! -z ${SKIPQC+x} ]; then + tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT} +else + tar -C $OUTPUT_DIR -zcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.result.tar.gz ${PROTOCOL}_${NAME_MT}_vs_${NAME_WT} ${PROTOCOL}_${NAME_MT} ${PROTOCOL}_${NAME_WT} +fi cp $PARAM_FILE $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.run.params echo -e "\nWorkflow end: `date`" From a432b77aa2b345bf9251d5a8346ea14a35568545 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 8 Apr 2019 15:36:29 +0000 Subject: [PATCH 04/32] Removing expected tree, will be adding new method for this soon --- .../contamination/COLO-829-BL.depthRG | 13468 ------ .../contamination/COLO-829-BL.depthSM | 202 - .../contamination/COLO-829-BL.log | 1 - .../contamination/COLO-829-BL.selfRG | 68 - .../contamination/COLO-829-BL.selfSM | 2 - .../contamination/COLO-829-BL_snps.vcf | 35408 ---------------- .../WGS_COLO-829-BL/contamination/result.json | 1 - .../contamination/COLO-829.depthRG | 20704 --------- .../contamination/COLO-829.depthSM | 202 - .../WGS_COLO-829/contamination/COLO-829.log | 1 - .../contamination/COLO-829.selfRG | 104 - .../contamination/COLO-829.selfSM | 2 - .../contamination/COLO-829_snps.vcf | 20961 --------- .../WGS_COLO-829/contamination/result.json | 1 - .../ascat/COLO-829.ASCATprofile.png | Bin 42504 -> 0 bytes .../ascat/COLO-829.ASPCF.png | Bin 411586 -> 0 bytes .../ascat/COLO-829.copynumber.baf.bw | 1 - .../ascat/COLO-829.copynumber.caveman.csv | 240 - .../ascat/COLO-829.copynumber.caveman.vcf.gz | Bin 4572 -> 0 bytes .../COLO-829.copynumber.caveman.vcf.gz.tbi | 1 - .../ascat/COLO-829.copynumber.logR.bw | 1 - .../ascat/COLO-829.copynumber.minorCn.bw | 1 - .../ascat/COLO-829.copynumber.rawCn.bw | 1 - .../ascat/COLO-829.copynumber.segBaf.bw | 1 - .../ascat/COLO-829.copynumber.segLogR.bw | 1 - .../ascat/COLO-829.copynumber.totalCn.bw | 1 - .../ascat/COLO-829.copynumber.txt.gz | 1 - .../ascat/COLO-829.germline.png | Bin 212694 -> 0 bytes .../ascat/COLO-829.rawprofile.png | Bin 55454 -> 0 bytes .../ascat/COLO-829.samplestatistics.txt | 7 - .../ascat/COLO-829.sunrise.png | Bin 35675 -> 0 bytes .../ascat/COLO-829.tumour.png | Bin 359125 -> 0 bytes .../brass/COLO-829-BL.brm.bam | 1 - .../brass/COLO-829-BL.brm.bam.bai | 1 - .../brass/COLO-829.brm.bam | 1 - .../brass/COLO-829.brm.bam.bai | 1 - .../COLO-829_vs_COLO-829-BL.annot.bedpe.gz | Bin 1997 -> 0 bytes ...COLO-829_vs_COLO-829-BL.annot.bedpe.gz.tbi | 1 - .../COLO-829_vs_COLO-829-BL.annot.vcf.gz | Bin 3636 -> 0 bytes .../COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi | 1 - ...LO-829_vs_COLO-829-BL.intermediates.tar.gz | 1 - .../COLO-829_vs_COLO-829-BL.ngscn.abs_cn.bw | 1 - .../COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz | Bin 9112 -> 0 bytes ...O-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi | 1 - ...OLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz | 1 - ...829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi | 1 - .../COLO-829_vs_COLO-829-BL.muts.ids.vcf.gz | 1 - ...OLO-829_vs_COLO-829-BL.muts.ids.vcf.gz.tbi | 1 - .../COLO-829_vs_COLO-829-BL.no_analysis.bed | 34944 --------------- .../COLO-829_vs_COLO-829-BL.snps.ids.vcf.gz | Bin 775266 -> 0 bytes ...OLO-829_vs_COLO-829-BL.snps.ids.vcf.gz.tbi | 1 - .../caveman/alg_bean | 4 - .../caveman/caveman.cfg.ini | 14 - .../caveman/cov_arr | Bin 454298 -> 0 bytes .../caveman/prob_arr | 1 - .../caveman/splitList | 3 - .../genotyped/COLO-829-BL.full_gender.tsv | 5 - .../genotyped/COLO-829-BL.full_genotype.tsv | 93 - .../genotyped/COLO-829.full_gender.tsv | 5 - .../genotyped/COLO-829.full_genotype.tsv | 93 - .../COLO-829_vs_COLO-829-BL.genotype.txt | 10 - .../genotyped/result.json | 1 - .../COLO-829_vs_COLO-829-BL.annot.vcf.gz | Bin 8670 -> 0 bytes .../COLO-829_vs_COLO-829-BL.annot.vcf.gz.tbi | 1 - .../COLO-829_vs_COLO-829-BL.flagged.vcf.gz | 1 - ...COLO-829_vs_COLO-829-BL.flagged.vcf.gz.tbi | 1 - .../COLO-829_vs_COLO-829-BL.germline.bed.gz | Bin 545 -> 0 bytes ...OLO-829_vs_COLO-829-BL.germline.bed.gz.tbi | 1 - .../pindel/COLO-829_vs_COLO-829-BL_mt.bam | Bin 35723 -> 0 bytes .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.bai | 1 - .../pindel/COLO-829_vs_COLO-829-BL_mt.bam.md5 | 1 - .../pindel/COLO-829_vs_COLO-829-BL_wt.bam | Bin 30326 -> 0 bytes .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.bai | 1 - .../pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 | 1 - 74 files changed, 126576 deletions(-) delete mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.depthRG delete mode 100644 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expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam delete mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.bai delete mode 100644 expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/pindel/COLO-829_vs_COLO-829-BL_wt.bam.md5 diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.depthRG b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.depthRG deleted file mode 100644 index 7dc0d13..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.depthRG +++ /dev/null @@ -1,13468 +0,0 @@ -#RG DEPTH #SNPs %SNPs %CUMUL -2649 200 0 0.00000 0.00000 -2649 199 0 0.00000 0.00000 -2649 198 0 0.00000 0.00000 -2649 197 0 0.00000 0.00000 -2649 196 0 0.00000 0.00000 -2649 195 0 0.00000 0.00000 -2649 194 0 0.00000 0.00000 -2649 193 0 0.00000 0.00000 -2649 192 0 0.00000 0.00000 -2649 191 0 0.00000 0.00000 -2649 190 0 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100644 index 8b13789..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.log +++ /dev/null @@ -1 +0,0 @@ - diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfRG b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfRG deleted file mode 100644 index 5ea6102..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfRG +++ /dev/null @@ -1,68 +0,0 @@ -#SEQ_ID RG CHIP_ID #SNPS #READS AVG_DP FREEMIX FREELK1 FREELK0 FREE_RH FREE_RA CHIPMIX CHIPLK1 CHIPLK0 CHIP_RH CHIP_RA DPREF RDPHET RDPALT -COLO-829-BL 2649 NA 35283 23521 0.67 0.00000 10151.12 10151.12 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2651 NA 35283 23048 0.65 0.00000 9854.90 9854.90 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2653 NA 35283 22765 0.65 0.00000 9577.72 9577.72 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2655 NA 35283 23010 0.65 0.00000 9987.93 9987.93 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2657 NA 35283 22380 0.63 0.00000 9789.86 9789.86 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2660 NA 35283 22364 0.63 0.00000 9603.49 9603.49 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2662 NA 35283 23135 0.66 0.00000 9803.81 9803.81 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2664 NA 35283 22828 0.65 0.00000 9687.63 9687.63 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2666 NA 35283 21791 0.62 0.00000 9446.68 9446.68 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2668 NA 35283 23114 0.66 0.00000 9806.33 9806.33 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2670 NA 35283 22914 0.65 0.00000 9864.51 9864.51 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2672 NA 35283 22522 0.64 0.00000 9659.06 9659.06 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2674 NA 35283 20898 0.59 0.00000 9172.71 9172.71 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2676 NA 35283 22615 0.64 0.00000 9621.23 9621.23 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2678 NA 35283 23512 0.67 0.00000 10001.17 10001.17 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2680 NA 35283 19802 0.56 0.00000 8568.90 8568.90 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2683 NA 35283 21866 0.62 0.00000 9357.06 9357.06 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2686 NA 35283 22593 0.64 0.00000 9875.72 9875.72 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2688 NA 35283 22382 0.63 0.00000 9705.37 9705.37 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2690 NA 35283 21914 0.62 0.00000 9393.00 9393.00 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2692 NA 35283 21475 0.61 0.00000 9236.75 9236.75 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2694 NA 35283 22350 0.63 0.00000 9823.47 9823.47 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2696 NA 35283 22368 0.63 0.00000 9685.83 9685.83 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2698 NA 35283 23428 0.66 0.00000 10156.39 10156.39 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2700 NA 35283 25028 0.71 0.00000 10802.39 10802.39 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2703 NA 35283 24814 0.70 0.00000 10428.29 10428.29 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2705 NA 35283 23996 0.68 0.00000 10269.03 10269.03 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2707 NA 35283 24546 0.70 0.00000 10413.96 10413.96 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2709 NA 35283 24747 0.70 0.00000 10565.20 10565.20 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2712 NA 35283 20661 0.59 0.00000 8928.14 8928.14 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2714 NA 35283 22318 0.63 0.00000 9709.99 9709.99 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2716 NA 35283 19032 0.54 0.00000 8348.44 8348.44 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2718 NA 35283 21872 0.62 0.00000 9421.76 9421.76 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2720 NA 35283 15939 0.45 0.00000 6965.54 6965.54 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2722 NA 35283 18407 0.52 0.00000 8049.76 8049.76 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2724 NA 35283 22054 0.63 0.00000 9468.13 9468.13 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2727 NA 35283 21526 0.61 0.00000 9304.01 9304.01 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2729 NA 35283 19194 0.54 0.00000 8278.21 8278.21 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2731 NA 35283 21456 0.61 0.00000 9403.38 9403.38 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2733 NA 35283 17864 0.51 0.00000 7830.53 7830.53 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2735 NA 35283 21201 0.60 0.00000 9077.09 9077.09 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2737 NA 35283 15358 0.44 0.00000 6809.59 6809.59 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2739 NA 35283 21685 0.61 0.00000 9419.21 9419.21 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2741 NA 35283 21094 0.60 0.00000 9062.19 9062.19 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2743 NA 35283 20274 0.57 0.00000 8912.35 8912.35 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2745 NA 35283 21927 0.62 0.00000 9519.33 9519.33 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2747 NA 35283 22450 0.64 0.00000 9584.08 9584.08 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2749 NA 35283 21377 0.61 0.00000 9295.24 9295.24 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2751 NA 35283 22390 0.63 0.00000 9562.52 9562.52 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2753 NA 35283 19785 0.56 0.00000 8642.89 8642.89 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2755 NA 35283 21633 0.61 0.00000 9430.88 9430.88 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2757 NA 35283 22485 0.64 0.00000 9681.00 9681.00 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2759 NA 35283 22527 0.64 0.00000 9740.99 9740.99 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2762 NA 35283 24958 0.71 0.00000 10649.54 10649.54 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2764 NA 35283 24581 0.70 0.00000 10365.04 10365.04 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2767 NA 35283 24253 0.69 0.00000 10348.05 10348.05 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2769 NA 35283 24453 0.69 0.00000 10321.45 10321.45 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2772 NA 35283 23534 0.67 0.00000 10112.26 10112.26 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2774 NA 35283 23461 0.66 0.00000 10083.68 10083.68 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2776 NA 35283 24907 0.71 0.00000 10460.70 10460.70 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2779 NA 35283 24558 0.70 0.00000 10413.08 10413.08 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2781 NA 35283 21801 0.62 0.00000 9339.48 9339.48 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2783 NA 35283 23326 0.66 0.00000 9874.64 9874.64 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2785 NA 35283 22665 0.64 0.00000 9652.76 9652.76 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2787 NA 35283 23602 0.67 0.00000 9966.43 9966.43 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2790 NA 35283 23681 0.67 0.00000 10074.88 10074.88 NA NA NA NA NA NA NA NA NA NA -COLO-829-BL 2792 NA 35283 23065 0.65 0.00000 9725.33 9725.33 NA NA NA NA NA NA NA NA NA NA diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfSM b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfSM deleted file mode 100644 index 463644d..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL.selfSM +++ /dev/null @@ -1,2 +0,0 @@ -#SEQ_ID RG CHIP_ID #SNPS #READS AVG_DP FREEMIX FREELK1 FREELK0 FREE_RH FREE_RA CHIPMIX CHIPLK1 CHIPLK0 CHIP_RH CHIP_RA DPREF RDPHET RDPALT -COLO-829-BL ALL NA 35283 1489080 42.20 0.00075 336467.25 336532.29 NA NA NA NA NA NA NA NA NA NA diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL_snps.vcf b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL_snps.vcf deleted file mode 100644 index cf49e8c..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829-BL/contamination/COLO-829-BL_snps.vcf +++ /dev/null @@ -1,35408 +0,0 @@ -#CHROM POS ID REF ALT QUAL FILTER INFO -1 1017216 rs6667248 A G . PASS AF=0.04188 -1 1311716 rs2649589 G A . PASS AF=0.77138 -1 1778090 rs12126768 T G . PASS AF=0.15348 -1 2069681 rs3753242 C T . PASS AF=0.19781 -1 2234251 rs1496555 A G . PASS AF=0.86161 -1 2328714 rs2645081 A G . PASS AF=0.84304 -1 2546229 rs6684865 G A . PASS AF=0.43266 -1 2831369 rs2606408 T C . PASS AF=0.05865 -1 2919942 rs12022429 G A . PASS AF=0.17887 -1 2974212 rs12057171 T C . PASS AF=0.02565 -1 3080855 rs1798246 A C . PASS AF=0.43709 -1 3138136 rs2817148 A G . PASS AF=0.13715 -1 3220160 rs6670518 A G . PASS AF=0.11327 -1 3256202 rs16824338 G A . PASS AF=0.04755 -1 3337552 rs2493283 T C . PASS AF=0.08286 -1 3494079 rs12080677 A G . PASS AF=0.22687 -1 3607520 rs3765730 G A . PASS AF=0.25503 -1 3696639 rs16824088 C T . PASS AF=0.02402 -1 3751929 rs12093010 G T . PASS AF=0.04990 -1 3999637 rs4654522 C G . PASS AF=0.33273 -1 4039440 rs12758410 C T . PASS AF=0.51987 -1 4111741 rs4553153 C T . PASS AF=0.25766 -1 4158734 rs12031803 T A . PASS AF=0.03986 -1 4220301 rs7549636 G A . PASS AF=0.24675 -1 4284659 rs903540 T C . PASS AF=0.68878 -1 4343581 rs16838549 G A . PASS AF=0.02398 -1 4370346 rs16838636 C A . PASS AF=0.01553 -1 4395020 rs12046130 G A . PASS AF=0.05761 -1 4426478 rs16838819 A C . PASS AF=0.18149 -1 4457112 rs16838929 C G . PASS AF=0.16709 -1 4482610 rs737324 G C . PASS AF=0.38945 -1 4523410 rs17453664 G A . PASS AF=0.12283 -1 4559484 rs616317 C A . PASS AF=0.07528 -1 4582089 rs518456 T G . PASS AF=0.35263 -1 4617856 rs10799244 T C . PASS AF=0.25289 -1 4657295 rs651695 A T . PASS AF=0.25761 -1 4693931 rs6687814 A G . PASS AF=0.40161 -1 4734514 rs12411035 A G . PASS AF=0.17888 -1 4774438 rs242058 T C . PASS AF=0.87270 -1 4812815 rs381240 A G . PASS AF=0.30488 -1 4853068 rs12408533 A G . PASS AF=0.03817 -1 4897644 rs11576972 G C . PASS AF=0.18922 -1 4958939 rs10915656 A G . PASS AF=0.22449 -1 4986435 rs4529681 G A . PASS AF=0.08583 -1 5011290 rs12403612 C G . PASS AF=0.07067 -1 5048624 rs7541612 T C . PASS AF=0.85640 -1 5088617 rs2169089 A G . PASS AF=0.76304 -1 5151476 rs2199215 T C . PASS AF=0.52212 -1 5198789 rs16840051 C T . PASS AF=0.34230 -1 5247355 rs10492931 G A . PASS AF=0.45884 -1 5303371 rs480807 G A . PASS AF=0.64359 -1 5330286 rs494138 C T . PASS AF=0.46626 -1 5362292 rs9439551 C G . PASS AF=0.38029 -1 5410542 rs4129058 C T . PASS AF=0.54484 -1 5532101 rs12566922 T G . PASS AF=0.14638 -1 5572076 rs10796398 G A . PASS AF=0.22742 -1 5625929 rs7523214 C G . PASS AF=0.05431 -1 5645712 rs4845805 G A . PASS AF=0.39912 -1 5684440 rs945321 C A . PASS AF=0.16142 -1 5718367 rs17028547 A C . PASS AF=0.02072 -1 5750582 rs770710 A T . PASS AF=0.16094 -1 5813724 rs12061902 T C . PASS AF=0.17633 -1 5902086 rs11579676 C T . PASS AF=0.21427 -1 5925955 rs12737596 T C . PASS AF=0.04300 -1 5985072 rs1011994 C T . PASS AF=0.14861 -1 6059322 rs7524865 G A . PASS AF=0.14348 -1 6141261 rs6701690 C G . PASS AF=0.10958 -1 6272813 rs2294711 C T . PASS AF=0.11485 -1 6416104 rs3789470 T C . PASS AF=0.10013 -1 6581283 rs12410405 C G . PASS AF=0.20773 -1 6713114 rs2235564 C T . PASS AF=0.31874 -1 6817343 rs17027008 T A . PASS AF=0.13993 -1 6927752 rs11120780 A G . PASS AF=0.63959 -1 7018719 rs11120797 A G . PASS AF=0.03063 -1 7089029 rs17030160 A C . PASS AF=0.01558 -1 7146809 rs9434834 T C . PASS AF=0.15858 -1 7214374 rs13376128 G T . PASS AF=0.22414 -1 7297400 rs7521056 A T . PASS AF=0.11816 -1 7336978 rs4908615 T A . PASS AF=0.43213 -1 7406419 rs4908456 T C . PASS AF=0.34129 -1 7449971 rs7524314 G A . PASS AF=0.29214 -1 7527996 rs1201529 T C . PASS AF=0.52139 -1 7554982 rs1725269 C T . PASS AF=0.70066 -1 7626995 rs17031145 C A . PASS AF=0.05192 -1 7697179 rs6661496 A T . PASS AF=0.09450 -1 7795554 rs4908688 C T . PASS AF=0.18933 -1 7850081 rs10864315 C T . PASS AF=0.26519 -1 7926542 rs504560 A G . PASS AF=0.64277 -1 8000562 rs9657956 C T . PASS AF=0.05221 -1 8161850 rs11487884 A G . PASS AF=0.08060 -1 8206326 rs6577473 C A . PASS AF=0.59929 -1 8273177 rs6683603 T G . PASS AF=0.32833 -1 8329710 rs2661869 C T . PASS AF=0.63665 -1 8457623 rs1443929 G T . PASS AF=0.15060 -1 8612104 rs6577499 G C . PASS AF=0.64178 -1 8767780 rs10864364 C A . PASS AF=0.85979 -1 8899305 rs6577532 C T . PASS AF=0.81602 -1 8993867 rs7417097 G A . PASS AF=0.30485 -1 9045124 rs7513804 C T . PASS AF=0.71809 -1 9116743 rs12080175 T C . PASS AF=0.30975 -1 9219383 rs6577556 T C . PASS AF=0.20481 -1 9292082 rs3753271 C T . PASS AF=0.30014 -1 9368209 rs1294040 G A . PASS AF=0.64682 -1 9417946 rs9435244 G A . PASS AF=0.10350 -1 9495587 rs9726405 T C . PASS AF=0.17077 -1 9650571 rs6669846 C T . PASS AF=0.02455 -1 9784423 rs11121484 C T . PASS AF=0.21830 -1 10013014 rs7516611 A C . PASS AF=0.15632 -1 10232200 rs17034569 C T . PASS AF=0.08874 -1 10353112 rs10492972 T C . PASS AF=0.32968 -1 10502710 rs2847337 A G . PASS AF=0.81899 -1 10591104 rs12090079 T C . PASS AF=0.07608 -1 10627542 rs12033275 G T . PASS AF=0.20859 -1 10726689 rs284321 G A . PASS AF=0.12132 -1 10832861 rs205474 C T . PASS AF=0.57717 -1 10929298 rs1281001 A G . PASS AF=0.59128 -1 11034817 rs9659356 A G . PASS AF=0.29462 -1 11191232 rs12732063 G A . PASS AF=0.01854 -1 11289161 rs2076655 G A . PASS AF=0.57495 -1 11401745 rs2982384 G A . PASS AF=0.37641 -1 11461601 rs6671983 G A . PASS AF=0.36952 -1 11507303 rs3010220 G A . PASS AF=0.02134 -1 11567190 rs2817637 C T . PASS AF=0.46179 -1 11647669 rs2817644 C T . PASS AF=0.31047 -1 11749908 rs6694489 C T . PASS AF=0.19377 -1 11828319 rs4845881 G A . PASS AF=0.65428 -1 11896654 rs198403 G A . PASS AF=0.12652 -1 11960394 rs17346334 T C . PASS AF=0.06100 -1 12117901 rs6665486 A G . PASS AF=0.46139 -1 12194612 rs1148471 G A . PASS AF=0.47178 -1 12249770 rs548580 G A . PASS AF=0.19639 -1 12418700 rs10864552 T C . PASS AF=0.94679 -1 12615069 rs6659122 G T . PASS AF=0.13976 -1 12672536 rs12046693 G C . PASS AF=0.21400 -1 12765166 rs4568844 C T . PASS AF=0.75593 -1 12813522 rs4548455 C T . PASS AF=0.05027 -1 13008676 rs3928862 G A . PASS AF=0.16698 -1 13841055 rs2990683 T C . PASS AF=0.65144 -1 13909142 rs355023 A C . PASS AF=0.63583 -1 13948047 rs12085273 G T . PASS AF=0.02610 -1 14043026 rs12049438 G A . PASS AF=0.17276 -1 14119085 rs1203642 T C . PASS AF=0.17732 -1 14150992 rs2697962 T C . PASS AF=0.82785 -1 14216150 rs17350970 C T . PASS AF=0.03696 -1 14252247 rs1534946 A G . PASS AF=0.12197 -1 14278991 rs7532844 T A . PASS AF=0.10033 -1 14313274 rs12728009 A G . PASS AF=0.18096 -1 14346018 rs7515012 C T . PASS AF=0.18178 -1 14380326 rs7519048 G C . PASS AF=0.14984 -1 14441384 rs7519796 A G . PASS AF=0.73920 -1 14474715 rs11586283 T C . PASS AF=0.33907 -1 14509276 rs577987 A G . PASS AF=0.15488 -1 14586318 rs10754915 T C . PASS AF=0.94610 -1 14655424 rs16853960 G A . PASS AF=0.04361 -1 14726096 rs820668 T C . PASS AF=0.96262 -1 14782380 rs6682103 T G . PASS AF=0.57814 -1 14811781 rs2480063 G A . PASS AF=0.45767 -1 14859579 rs7540545 T C . PASS AF=0.39903 -1 14919895 rs664747 G A . 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PASS AF=0.18944 -1 16716374 rs612727 T C . PASS AF=0.73533 -1 16814994 rs156851 C T . PASS AF=0.93350 -1 17290058 rs6696928 A G . PASS AF=0.94176 -1 17386104 rs12404121 C T . PASS AF=0.55366 -1 17409241 rs2235925 G A . PASS AF=0.41606 -1 17465174 rs2746486 A G . PASS AF=0.53898 -1 17514734 rs3003464 C G . PASS AF=0.25143 -1 17557837 rs3003409 A C . PASS AF=0.39339 -1 17595925 rs17458091 T C . PASS AF=0.08699 -1 17655407 rs1748041 C T . PASS AF=0.63610 -1 17694701 rs1204890 T G . PASS AF=0.28934 -1 17785736 rs2428740 C T . PASS AF=0.34500 -1 17903298 rs6702679 G A . PASS AF=0.32566 -1 18003946 rs6665721 C T . PASS AF=0.02171 -1 18079812 rs12136469 C T . PASS AF=0.22986 -1 18143674 rs508571 C T . PASS AF=0.33696 -1 18201318 rs563835 T C . PASS AF=0.48431 -1 18246140 rs12073092 T A . PASS AF=0.31540 -1 18282095 rs1192583 G A . PASS AF=0.93892 -1 18317841 rs12726822 G A . PASS AF=0.20157 -1 18343106 rs12058837 G T . PASS AF=0.14041 -1 18374838 rs4920416 T C . 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--git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.log b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.log deleted file mode 100644 index 8b13789..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.log +++ /dev/null @@ -1 +0,0 @@ - diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfRG b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfRG deleted file mode 100644 index 383f6de..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfRG +++ /dev/null @@ -1,104 +0,0 @@ -#SEQ_ID RG CHIP_ID #SNPS #READS AVG_DP FREEMIX FREELK1 FREELK0 FREE_RH FREE_RA CHIPMIX CHIPLK1 CHIPLK0 CHIP_RH CHIP_RA DPREF RDPHET RDPALT -COLO-829 2650 NA 20887 14834 0.71 0.00000 6244.71 6244.71 NA NA NA NA NA NA NA NA NA NA -COLO-829 2652 NA 20887 14397 0.69 0.00000 6102.14 6102.14 NA NA NA NA NA NA NA NA NA NA -COLO-829 2654 NA 20887 13808 0.66 0.00000 5928.59 5928.59 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20887 14994 0.72 0.00000 6367.85 6367.85 NA NA NA NA NA NA NA NA NA NA -COLO-829 2799 NA 20887 11700 0.56 0.00000 5095.41 5095.41 NA NA NA NA NA NA NA NA NA NA -COLO-829 2800 NA 20887 14581 0.70 0.00000 6280.03 6280.03 NA NA NA NA NA NA NA NA NA NA -COLO-829 2801 NA 20887 10890 0.52 0.00000 4813.41 4813.41 NA NA NA NA NA NA NA NA NA NA -COLO-829 2802 NA 20887 14401 0.69 0.00000 6169.91 6169.91 NA NA NA NA NA NA NA NA NA NA -COLO-829 2803 NA 20887 9380 0.45 0.00000 4123.90 4123.90 NA NA NA NA NA NA NA NA NA NA -COLO-829 2804 NA 20887 12460 0.60 0.00000 5434.13 5434.13 NA NA NA NA NA NA NA NA NA NA -COLO-829 2805 NA 20887 13473 0.65 0.00000 5660.14 5660.14 NA NA NA NA NA NA NA NA NA NA -COLO-829 2806 NA 20887 10204 0.49 0.00000 4553.81 4553.81 NA NA NA NA NA NA NA NA NA NA -COLO-829 2807 NA 20887 9366 0.45 0.00000 4117.81 4117.81 NA NA NA NA NA NA NA NA NA NA -COLO-829 2808 NA 20887 10297 0.49 0.00000 4516.26 4516.26 NA NA NA NA NA NA NA NA NA NA -COLO-829 2809 NA 20887 10244 0.49 0.00000 4561.70 4561.70 NA NA NA NA NA NA NA NA NA NA -COLO-829 76 NA 20887 3979 0.19 0.00000 1899.07 1899.07 NA NA NA NA NA NA NA NA NA NA -COLO-829 77 NA 20887 4180 0.20 0.00000 1879.38 1879.38 NA NA NA NA NA NA NA NA NA NA -COLO-829 78 NA 20887 3241 0.16 0.01277 1465.91 1465.93 NA NA NA NA NA NA NA NA NA NA -COLO-829 81 NA 20887 3829 0.18 0.00000 1760.72 1760.72 NA NA NA NA NA NA NA NA NA NA -COLO-829 994 NA 20887 3297 0.16 0.07082 1444.92 1445.34 NA NA NA NA NA NA NA NA NA NA -COLO-829 995 NA 20887 2891 0.14 0.00000 1279.04 1279.04 NA NA NA NA NA NA NA NA NA NA -COLO-829 996 NA 20887 3304 0.16 0.00000 1497.73 1497.73 NA NA NA NA NA NA NA NA NA NA -COLO-829 997 NA 20887 3409 0.16 0.00000 1465.42 1465.42 NA NA NA NA NA NA NA NA NA NA -COLO-829 998 NA 20887 3280 0.16 0.00000 1437.91 1437.91 NA NA NA NA NA NA NA NA NA NA -COLO-829 999 NA 20887 3364 0.16 0.11522 1471.54 1472.46 NA NA NA NA NA NA NA NA NA NA diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfSM b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfSM deleted file mode 100644 index d673731..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829.selfSM +++ /dev/null @@ -1,2 +0,0 @@ -#SEQ_ID RG CHIP_ID #SNPS #READS AVG_DP FREEMIX FREELK1 FREELK0 FREE_RH FREE_RA CHIPMIX CHIPLK1 CHIPLK0 CHIP_RH CHIP_RA DPREF RDPHET RDPALT -COLO-829 ALL NA 20887 1234173 59.09 0.00109 272092.96 272205.96 NA NA NA NA NA NA NA NA NA NA diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829_snps.vcf b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829_snps.vcf deleted file mode 100644 index bbe9eb6..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829/contamination/COLO-829_snps.vcf +++ /dev/null @@ -1,20961 +0,0 @@ -#CHROM POS ID REF ALT QUAL FILTER INFO -1 144551842 rs1699721 C T . PASS AF=0.95000 -1 144945737 rs2996281 G T . PASS AF=0.68029 -1 145041016 rs2799329 G A . PASS AF=0.05000 -2 41404 rs10173732 G A . PASS AF=0.18223 -2 151464 rs381726 T C . PASS AF=0.51279 -2 210076 rs11692330 G C . PASS AF=0.34338 -2 282736 rs6708541 G T . PASS AF=0.67819 -2 355800 rs6740249 T C . PASS AF=0.68073 -2 454860 rs1511324 G A . PASS AF=0.25911 -2 548121 rs2685261 C T . PASS AF=0.73483 -2 589743 rs2683980 G A . PASS AF=0.11729 -2 614168 rs2947411 A G . PASS AF=0.84001 -2 637830 rs13393304 A G . PASS AF=0.87828 -2 669023 rs3187671 C T . PASS AF=0.11917 -2 759749 rs7596247 A G . PASS AF=0.27573 -2 825721 rs4358155 A C . PASS AF=0.85572 -2 877633 rs12466616 G A . PASS AF=0.52501 -2 943250 rs4971346 C T . PASS AF=0.26955 -2 1025239 rs10201045 A G . PASS AF=0.71192 -2 1077718 rs10470000 T C . PASS AF=0.99900 -2 1173710 rs4464311 T C . PASS AF=0.58960 -2 1311760 rs9678456 T C . PASS AF=0.07164 -2 1383237 rs4927650 T G . PASS AF=0.68697 -2 1432599 rs949834 G A . PASS AF=0.08284 -2 1473898 rs11897718 T A . PASS AF=0.22705 -2 1620721 rs12614899 C T . PASS AF=0.20106 -2 1696016 rs2288183 G A . PASS AF=0.62480 -2 1772199 rs13403413 G A . PASS AF=0.25325 -2 1838964 rs6747366 A G . PASS AF=0.50786 -2 1897522 rs12476079 G C . PASS AF=0.08354 -2 1974582 rs1864882 T G . PASS AF=0.51705 -2 2058426 rs6716149 C T . PASS AF=0.04490 -2 2204863 rs12471456 G A . PASS AF=0.28694 -2 2268764 rs6753548 C G . PASS AF=0.64530 -2 2355993 rs2551214 G T . PASS AF=0.57480 -2 2410541 rs2063549 C T . PASS AF=0.25024 -2 2474047 rs1706869 G T . PASS AF=0.08784 -2 2520736 rs1710503 C T . PASS AF=0.61866 -2 2538135 rs17039995 G A . PASS AF=0.02618 -2 2583815 rs10166891 T C . PASS AF=0.17379 -2 2608466 rs17496843 C T . PASS AF=0.17934 -2 2643137 rs7591104 C G . PASS AF=0.38903 -2 2687911 rs1470738 A G . PASS AF=0.61086 -2 2740902 rs9309697 A G . PASS AF=0.86916 -2 2787699 rs4146909 T C . PASS AF=0.67053 -2 2837549 rs11686670 C A . PASS AF=0.24521 -2 2872628 rs876299 A G . PASS AF=0.35077 -2 2898776 rs6724661 A G . PASS AF=0.03476 -2 2944589 rs935626 A G . PASS AF=0.05100 -2 3001674 rs1729936 C T . PASS AF=0.56846 -2 3084999 rs6754010 T C . PASS AF=0.05000 -2 3129553 rs17040861 G A . PASS AF=0.13359 -2 3193040 rs17682 G A . PASS AF=0.24028 -2 3242858 rs6548144 C T . PASS AF=0.91382 -2 3280842 rs2462956 C T . PASS AF=0.61137 -2 3371308 rs10189709 G C . PASS AF=0.68827 -2 3457097 rs10193454 G A . PASS AF=0.19286 -2 3511044 rs12052249 C A . PASS AF=0.30512 -2 3665146 rs11896016 T C . PASS AF=0.66625 -2 3702733 rs11902059 A G . PASS AF=0.09878 -2 3732546 rs6743352 T C . PASS AF=0.15799 -2 3780896 rs357953 C A . PASS AF=0.31458 -2 3815767 rs4849927 G A . PASS AF=0.68382 -2 3841699 rs12466058 T C . PASS AF=0.57616 -2 3896630 rs1902020 A C . PASS AF=0.25202 -2 3929278 rs7592385 A G . PASS AF=0.49176 -2 4030608 rs1494055 G T . PASS AF=0.33029 -2 4088831 rs12467317 G A . PASS AF=0.11411 -2 4137992 rs6759055 T G . PASS AF=0.10721 -2 4186566 rs4850056 T G . PASS AF=0.26714 -2 4270966 rs10204732 C T . PASS AF=0.21922 -2 4313449 rs7606619 T G . PASS AF=0.04784 -2 4352168 rs17552614 A C . PASS AF=0.08816 -2 4427111 rs11897967 A G . PASS AF=0.12831 -2 4502958 rs654853 T G . PASS AF=0.61706 -2 4583303 rs17562653 A G . PASS AF=0.03849 -2 4607508 rs6717561 C G . PASS AF=0.25649 -2 4659821 rs13006169 A G . PASS AF=0.16464 -2 4724464 rs10208517 C A . PASS AF=0.07951 -2 4773653 rs987689 C G . PASS AF=0.04467 -2 4822438 rs13395076 G A . PASS AF=0.12013 -2 4853967 rs1439818 A C . PASS AF=0.37512 -2 4894001 rs17020102 A G . PASS AF=0.07035 -2 4929220 rs1371485 G A . PASS AF=0.21523 -2 4973754 rs12711964 A G . PASS AF=0.46424 -2 5205484 rs12466042 C T . PASS AF=0.10444 -2 5253908 rs6720425 G A . PASS AF=0.05325 -2 5293171 rs17331611 C A . PASS AF=0.05168 -2 5377640 rs17333600 A G . PASS AF=0.42806 -2 5418064 rs1453783 C T . PASS AF=0.53083 -2 5456009 rs6752772 A T . PASS AF=0.69376 -2 5511339 rs16863669 C T . PASS AF=0.06308 -2 5547463 rs1659014 G C . PASS AF=0.94664 -2 5588619 rs16863779 T C . PASS AF=0.09016 -2 5645201 rs12614734 T C . PASS AF=0.53370 -2 5700470 rs13036193 A G . PASS AF=0.12032 -2 5773869 rs10195195 C A . PASS AF=0.04161 -2 5821748 rs4547512 G T . PASS AF=0.74475 -2 5870314 rs16864077 G A . PASS AF=0.04686 -2 5921095 rs16864193 C T . PASS AF=0.05000 -2 5968922 rs995949 A C . PASS AF=0.35181 -2 6002657 rs12471085 C T . PASS AF=0.15341 -2 6040560 rs4668813 A G . PASS AF=0.88704 -2 6072487 rs12470700 C T . PASS AF=0.13850 -2 6117771 rs12623457 T A . PASS AF=0.28371 -2 6165022 rs16864588 T C . PASS AF=0.01168 -2 6204312 rs12992673 G A . PASS AF=0.24981 -2 6247942 rs4233842 T A . PASS AF=0.91947 -2 6297054 rs6738047 G A . PASS AF=0.17991 -2 6343942 rs11897219 A G . PASS AF=0.02500 -2 6381852 rs10495535 G A . PASS AF=0.03955 -2 6398894 rs6734659 T A . PASS AF=0.82854 -2 6443516 rs921227 G A . PASS AF=0.61601 -2 6496322 rs16865067 G T . PASS AF=0.10499 -2 6550266 rs1462060 G A . PASS AF=0.13385 -2 6580645 rs3101047 C T . PASS AF=0.37607 -2 6622471 rs12475855 A T . PASS AF=0.29136 -2 6655781 rs16865292 T C . PASS AF=0.05327 -2 6701257 rs2061315 A G . PASS AF=0.47396 -2 6753497 rs11884506 G A . PASS AF=0.05000 -2 6794825 rs7564462 T G . PASS AF=0.43025 -2 6857712 rs6725067 T C . PASS AF=0.42506 -2 6891670 rs6721103 T C . PASS AF=0.14217 -2 6948715 rs771177 T C . PASS AF=0.95000 -2 7007620 rs2122103 C G . PASS AF=0.94399 -2 7069534 rs11674053 G A . PASS AF=0.01087 -2 7101720 rs771318 A G . PASS AF=0.02314 -2 7150330 rs447721 C T . PASS AF=0.41743 -2 7187738 rs7575969 A G . PASS AF=0.17086 -2 7229091 rs6431847 T C . PASS AF=0.66016 -2 7263133 rs10221865 G A . PASS AF=0.21458 -2 7322437 rs12151441 T C . PASS AF=0.18653 -2 7350916 rs4669156 C A . PASS AF=0.29344 -2 7407463 rs16866052 G T . PASS AF=0.27630 -2 7471732 rs6732990 G A . PASS AF=0.50513 -2 7533435 rs6756247 G A . PASS AF=0.50075 -2 7586436 rs11897766 T C . PASS AF=0.03812 -2 7647860 rs1907451 G T . PASS AF=0.21387 -2 7714913 rs6710248 T C . PASS AF=0.38644 -2 7812716 rs11682611 C T . PASS AF=0.15396 -2 7869555 rs772431 C T . PASS AF=0.95981 -2 7942491 rs7570944 T C . PASS AF=0.20037 -2 7997419 rs1861500 T C . PASS AF=0.68291 -2 8045363 rs16866529 T C . PASS AF=0.46225 -2 8094445 rs42208 C T . PASS AF=0.95000 -2 8115782 rs1974696 G A . PASS AF=0.53508 -2 8161538 rs16866571 A T . PASS AF=0.14358 -2 8227802 rs6759347 A C . PASS AF=0.46412 -2 8283740 rs6735900 G T . PASS AF=0.17881 -2 8373273 rs1034381 T C . PASS AF=0.60006 -2 8436099 rs346830 A G . PASS AF=0.32265 -2 8466486 rs10210006 C G . PASS AF=0.31655 -2 8501738 rs3097283 T C . PASS AF=0.82707 -2 8549910 rs6744484 A G . PASS AF=0.09584 -2 8579305 rs876861 G A . PASS AF=0.13562 -2 8613081 rs6739697 T C . PASS AF=0.10287 -2 8667160 rs6712259 A G . PASS AF=0.69392 -2 8707402 rs345898 C T . PASS AF=0.06196 -2 8753269 rs12993630 C T . PASS AF=0.41669 -2 8810012 rs3856461 C T . PASS AF=0.76617 -2 8927209 rs2033755 C T . PASS AF=0.95842 -2 9076600 rs7596539 C T . PASS AF=0.07031 -2 9226881 rs328628 C G . PASS AF=0.72862 -2 9274692 rs12622209 C A . PASS AF=0.57889 -2 9328737 rs7582342 G A . PASS AF=0.33991 -2 9398844 rs2709590 A G . PASS AF=0.48675 -2 9449556 rs6715973 G A . PASS AF=0.05734 -2 9503165 rs4669386 G A . PASS AF=0.22443 -2 9574542 rs12464664 T C . PASS AF=0.44989 -2 9662820 rs10929587 T A . PASS AF=0.51541 -2 9770050 rs13417081 A G . PASS AF=0.50379 -2 9807984 rs6713719 C T . PASS AF=0.12039 -2 9863969 rs12466375 G A . PASS AF=0.46238 -2 9908191 rs16867157 C T . PASS AF=0.04292 -2 9985606 rs287986 T C . PASS AF=0.17170 -2 10046283 rs4669490 A C . PASS AF=0.51479 -2 10098227 rs6708858 T C . PASS AF=0.45760 -2 10163328 rs10803714 T C . PASS AF=0.74683 -2 10275814 rs717886 T C . PASS AF=0.69530 -2 10364272 rs16855971 C T . PASS AF=0.21964 -2 10413431 rs17379538 T C . PASS AF=0.33045 -2 10468296 rs1003652 A C . PASS AF=0.20545 -2 10519847 rs12477018 G A . PASS AF=0.19458 -2 10566804 rs2270307 A G . PASS AF=0.21516 -2 10640667 rs11686084 G A . PASS AF=0.06033 -2 10688969 rs4668687 T G . PASS AF=0.54211 -2 10734703 rs11680669 T C . PASS AF=0.05014 -2 10845437 rs6738662 C G . PASS AF=0.02290 -2 10922516 rs1686482 A C . PASS AF=0.38370 -2 10943882 rs1734365 A G . PASS AF=0.44218 -2 10986305 rs1734394 C T . PASS AF=0.60591 -2 11018001 rs2884310 G A . PASS AF=0.75607 -2 11033567 rs4233891 T G . PASS AF=0.78153 -2 11085961 rs6735622 C G . PASS AF=0.35715 -2 11135121 rs10185930 G A . PASS AF=0.67054 -2 11162735 rs1349722 C T . PASS AF=0.33021 -2 11258601 rs11892684 G A . PASS AF=0.05675 -2 11366000 rs10167277 T A . PASS AF=0.38573 -2 11509480 rs4668722 G A . PASS AF=0.75652 -2 11562669 rs6708015 T C . PASS AF=0.37061 -2 11649547 rs2082830 G A . PASS AF=0.95818 -2 11706730 rs13383579 C T . PASS AF=0.05000 -2 11753955 rs10174816 T C . PASS AF=0.11310 -2 11815723 rs11676470 G T . PASS AF=0.13260 -2 11872755 rs6723479 T C . PASS AF=0.01577 -2 11938946 rs2577262 G A . PASS AF=0.35863 -2 12017371 rs893341 C T . PASS AF=0.81988 -2 12069382 rs2581084 T G . PASS AF=0.41315 -2 12110926 rs6712004 T C . PASS AF=0.42622 -2 12146345 rs7565113 T C . PASS AF=0.27709 -2 12201097 rs10929783 G C . PASS AF=0.24947 -2 12242056 rs6432262 T C . PASS AF=0.73327 -2 12272871 rs10201223 A C . PASS AF=0.33915 -2 12304843 rs3863945 G C . PASS AF=0.24243 -2 12326837 rs17618039 A T . PASS AF=0.37096 -2 12363549 rs10929794 G A . PASS AF=0.23819 -2 12418532 rs6432275 C T . PASS AF=0.88380 -2 12451586 rs1453490 C G . PASS AF=0.05000 -2 12495667 rs4417692 C T . PASS AF=0.05760 -2 12568996 rs10929808 G A . PASS AF=0.79180 -2 12624618 rs12614015 C T . PASS AF=0.49376 -2 12678708 rs16859063 A C . PASS AF=0.04673 -2 12745377 rs6729948 T C . PASS AF=0.02164 -2 12791196 rs6746789 A G . PASS AF=0.04840 -2 12855750 rs7593863 A G . PASS AF=0.06448 -2 12898480 rs13400230 T C . PASS AF=0.05000 -2 12969972 rs7558818 C T . PASS AF=0.61856 -2 13069079 rs7607052 C T . PASS AF=0.49573 -2 13126278 rs7590964 A G . PASS AF=0.31416 -2 13202306 rs11678695 G T . PASS AF=0.14675 -2 13310106 rs10182726 G A . PASS AF=0.34464 -2 13392540 rs388556 C G . PASS AF=0.28923 -2 13549728 rs10182743 A G . PASS AF=0.13245 -2 13617364 rs10495616 G A . PASS AF=0.27591 -2 13676862 rs2140525 A G . PASS AF=0.69861 -2 13698137 rs4669937 A G . PASS AF=0.69140 -2 13792992 rs1510832 G A . PASS AF=0.13877 -2 13863284 rs6432409 G A . PASS AF=0.74476 -2 13955627 rs7592520 C T . PASS AF=0.13996 -2 14056291 rs6742948 C T . PASS AF=0.35433 -2 14126218 rs12612782 C T . PASS AF=0.05988 -2 14204517 rs16861144 A T . PASS AF=0.02068 -2 14291599 rs7355592 G A . PASS AF=0.09892 -2 14332903 rs7600508 A G . PASS AF=0.46007 -2 14373024 rs16861506 C T . PASS AF=0.07195 -2 14433542 rs11684967 G A . PASS AF=0.23486 -2 14505949 rs10171046 T G . PASS AF=0.55445 -2 14571744 rs4145975 T C . PASS AF=0.44785 -2 14658959 rs6745064 G A . PASS AF=0.56939 -2 14696966 rs2675896 G C . PASS AF=0.67694 -2 14759577 rs16861922 G C . PASS AF=0.05257 -2 14851012 rs1434983 G C . PASS AF=0.08831 -2 14911179 rs10929918 G T . PASS AF=0.28127 -2 15000512 rs7570741 C T . PASS AF=0.06644 -2 15043800 rs4670039 G A . PASS AF=0.13109 -2 15091566 rs17368670 A C . PASS AF=0.16720 -2 15133451 rs10495643 C T . PASS AF=0.13434 -2 15181133 rs6720338 T C . PASS AF=0.34935 -2 15203160 rs1367489 C T . PASS AF=0.49166 -2 15270541 rs4668873 A G . PASS AF=0.88718 -2 15326834 rs2302940 T C . PASS AF=0.08263 -2 15409565 rs735447 A G . PASS AF=0.20144 -2 15497974 rs6727262 G A . PASS AF=0.70764 -2 15601441 rs7590340 C T . PASS AF=0.55127 -2 15722503 rs13391772 A G . PASS AF=0.53607 -2 15787986 rs807616 A G . PASS AF=0.99510 -2 15818041 rs7582338 A G . PASS AF=0.16074 -2 15867762 rs4668964 C T . PASS AF=0.20597 -2 15906778 rs2544535 T C . PASS AF=0.56530 -2 15944021 rs10185339 C T . PASS AF=0.09042 -2 15986586 rs11678237 C A . PASS AF=0.27315 -2 16040430 rs2081782 C T . PASS AF=0.00100 -2 16137237 rs3213827 G A . PASS AF=0.57285 -2 16199265 rs10173113 G A . PASS AF=0.27502 -2 16394600 rs13018508 C T . PASS AF=0.04299 -2 16435385 rs16982180 A G . PASS AF=0.14966 -2 16471243 rs340742 C A . PASS AF=0.69614 -2 16545353 rs6761668 A G . PASS AF=0.06015 -2 16606596 rs4832591 A G . PASS AF=0.80419 -2 16644988 rs11096574 T G . PASS AF=0.68678 -2 16680691 rs1430054 A G . PASS AF=0.80269 -2 16734284 rs12997119 T C . PASS AF=0.13911 -2 16803169 rs10188832 T C . PASS AF=0.36735 -2 16826386 rs6727449 T C . PASS AF=0.03403 -2 16896822 rs17645454 C T . PASS AF=0.16373 -2 16949316 rs11691792 A C . PASS AF=0.18156 -2 16983700 rs500053 T C . PASS AF=0.55870 -2 17037809 rs6727942 G T . PASS AF=0.41711 -2 17121628 rs2287517 A C . PASS AF=0.29777 -2 17222830 rs2880387 T A . PASS AF=0.35885 -2 17303956 rs10495659 A G . PASS AF=0.25631 -2 17369844 rs287295 C T . PASS AF=0.68407 -2 17444663 rs16983422 A G . PASS AF=0.11660 -2 17465777 rs16983448 T C . PASS AF=0.02820 -2 17535528 rs16983540 C T . PASS AF=0.01101 -2 17662826 rs17380058 A G . PASS AF=0.15819 -2 17774634 rs3109180 A G . PASS AF=0.67601 -2 17834452 rs6751113 C T . PASS AF=0.38648 -2 17953560 rs300174 C T . PASS AF=0.70023 -2 17997581 rs1471344 T C . PASS AF=0.57227 -2 18052674 rs10178489 C G . PASS AF=0.34528 -2 18117930 rs2068801 C T . PASS AF=0.05000 -2 18151088 rs2709998 T C . PASS AF=0.97366 -2 18227760 rs16984221 T C . PASS AF=0.11984 -2 18270965 rs7426132 G A . PASS AF=0.13350 -2 18294360 rs2345494 C T . PASS AF=0.96840 -2 18352405 rs10495679 G T . PASS AF=0.05000 -2 18400797 rs10171254 A G . PASS AF=0.87651 -2 18451405 rs16984606 T C . PASS AF=0.15114 -2 18505648 rs10186797 A G . PASS AF=0.02939 -2 18538810 rs7559226 C T . PASS AF=0.56682 -2 18600650 rs10179711 G A . PASS AF=0.28023 -2 18653880 rs2704625 T C . PASS AF=0.90314 -2 18677655 rs4119151 G A . PASS AF=0.22153 -2 18712884 rs17751326 A G . 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a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi deleted file mode 100644 index 8b13789..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.annot.muts.vcf.gz.tbi +++ /dev/null @@ -1 +0,0 @@ - diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz deleted file mode 100644 index 8b13789..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz +++ /dev/null @@ -1 +0,0 @@ - diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/COLO-829_vs_COLO-829-BL.flagged.muts.vcf.gz.tbi 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@@ -rd_pos 2.63;47.37;23.68;13.16;13.16 -base_qual 0-9;10-19;20-24;25-29;30-34;35-39;40-200 -map_qual 0-60;255-255;61-254 -lane 2649_1;2651_1;2653_1;2655_1;2657_1;2660_1;2662_1;2664_1;2666_1;2668_1;2670_1;2672_1;2674_1;2676_1;2678_1;2680_1;2683_1;2686_1;2688_1;2690_1;2692_1;2694_1;2696_1;2698_1;2700_1;2703_1;2705_1;2707_1;2709_1;2712_1;2714_1;2716_1;2718_1;2720_1;2722_1;2724_1;2727_1;2729_1;2731_1;2733_1;2735_1;2737_1;2739_1;2741_1;2743_1;2745_1;2747_1;2749_1;2751_1;2753_1;2755_1;2757_1;2759_1;2762_1;2764_1;2767_1;2769_1;2772_1;2774_1;2776_1;2779_1;2781_1;2783_1;2785_1;2787_1;2790_1;2792_1;2650_0;2652_0;2654_0;2656_0;2658_0;2659_0;2661_0;2663_0;2665_0;2667_0;2669_0;2671_0;2673_0;2675_0;2677_0;2679_0;2681_0;2684_0;2685_0;2687_0;2689_0;2691_0;2693_0;2695_0;2697_0;2699_0;2701_0;2702_0;2704_0;2706_0;2708_0;2710_0;2711_0;2713_0;2715_0;2717_0;2719_0;2721_0;2723_0;2725_0;2726_0;2728_0;2730_0;2732_0;2734_0;2736_0;2738_0;2740_0;2742_0;2744_0;2746_0;2748_0;2750_0;2752_0;2754_0;2756_0;2758_0;2760_0;2761_0;2763_0;2765_0;2766_0;2768_0;2770_0;2771_0;2773_0;2775_0;2777_0;2778_0;2780_0;2782_0;2784_0;2786_0;2788_0;2789_0;2791_0;2793_0;2794_0;2795_0;2796_0;2797_0;2798_0;2799_0;2800_0;2801_0;2802_0;2803_0;2804_0;2805_0;2806_0;2807_0;2808_0;2809_0;76_0;77_0;78_0;81_0;994_0;995_0;996_0;997_0;998_0;999_0 diff --git a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini b/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini deleted file mode 100644 index 7648e75..0000000 --- a/expected/cgpwgs/bam_bai_pu_pl/WGS_COLO-829_vs_COLO-829-BL/caveman/caveman.cfg.ini +++ /dev/null @@ -1,14 +0,0 @@ -CWD=/var/spool/cwl -MUT_BAM=/var/spool/cwl/tmp/COLO-829.bam -NORM_BAM=/var/spool/cwl/tmp/COLO-829-BL.bam -REF_IDX=/var/spool/cwl/reference_files/genome.fa.fai -IGNORE=/var/spool/cwl/reference_files/caveman/HiDepth.tsv -ALG_FILE=/var/spool/cwl/WGS_COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/alg_bean -RESULT_DIR=/var/spool/cwl/WGS_COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/results -SPLIT_FILE=/var/spool/cwl/WGS_COLO-829_vs_COLO-829-BL/caveman/tmpCaveman/splitList -SW=0 -SE=0 -DUP=0 -NORMCN=/var/spool/cwl/tmp/norm.cn.bed -TUMCN=/var/spool/cwl/tmp/tum.cn.bed -VER=1.13.2 diff --git 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ziAT&XcuJHx+Y!@va?0FUQ{;*{uN~(={|%IMn>CLrogAN!rc}_nV*Wuz;fB zGDag~Oq$87W9ep@nGy?TOXP#&Lty^{pqD)8;S#y&nN#~03}^*b^@>i+US7dGnpX$N ztDPBoLv%X~WC+@HT1fxEDX$c()@-@gzq-foCj*tZ2Bo5c54rzi6o7fdW8%u;6p+dE zPu3QGk)*h_<@r(4{nD@BfzW*SBgYRzHasOwWi6P*Ub1xEvb0L>XJ>BTJLtI{G<1t? z(sk+3kzPd!dpU9ZgOQdn*N;CM&#p&$GyFy44%$1bvjfW&ouQFHEf zPwwV;UbW8Wsn+wphn7*#=X3lTzl_taX&Pv|y2fS!n;OUc}``Xtb{e0?? z<7Sm7r=OxOImjP~OIa0e@=+W-?e@Ce7} Date: Mon, 8 Apr 2019 15:41:22 +0000 Subject: [PATCH 05/32] Update readme, cleanout 'expected' etc --- README.md | 84 +++++++++++++++++++++++++------------------------------ 1 file changed, 38 insertions(+), 46 deletions(-) diff --git a/README.md b/README.md index 6f1c7a4..7c774e8 100644 --- a/README.md +++ b/README.md @@ -12,21 +12,17 @@ the [Dockstore.org](https://dockstore.org/) framework. | --------------------------------------------- | ----------------------------------------------- | | [![Master Badge][travis-master]][travis-base] | [![Develop Badge][travis-develop]][travis-base] | - - -- [Usage](#usage) - - [Usable Cores](#usable-cores) -- [Other uses](#other-uses) - - [Native docker](#native-docker) - - [Singularity](#singularity) -- [Verifying your deployment](#verifying-your-deployment) -- [Example data](#example-data) -- [Diagram of internals](#diagram-of-internals) -- [Development environment](#development-environment) -- [Release process](#release-process) -- [LICENCE](#licence) - - +* [Usage](#usage) + * [Usable Cores](#usable-cores) +* [Other uses](#other-uses) + * [Native docker](#native-docker) + * [Singularity](#singularity) +* [Verifying your deployment](#verifying-your-deployment) +* [Example data](#example-data) +* [Diagram of internals](#diagram-of-internals) +* [Development environment](#development-environment) +* [Release process](#release-process) +* [LICENCE](#licence) ## Usage @@ -40,14 +36,14 @@ You should see the usage for the `ds-cgpwgs.pl` script for all parameters (or th Required input files are 1. Tumour BAM file -1. Normal BAM file -1. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz][ftp-ref]) -1. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz][ftp-ref]) -1. WGS reference archive (e.g. [CNV_SV_ref_GRCh37d5_brass6+.tar.gz][ftp-ref]) -1. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz][ftp-ref]) -1. Subclonal reference archive ([SUBCL_ref_GRCh37d5.tar.gz][ftp-ref]) +2. Normal BAM file +3. Core reference archive (e.g. [core_ref_GRCh37d5.tar.gz][ftp-ref]) +4. WXS reference archive (e.g. [SNV_INDEL_ref_GRCh37d5.tar.gz][ftp-ref]) +5. WGS reference archive (e.g. [CNV_SV_ref_GRCh37d5_brass6+.tar.gz][ftp-ref]) +6. VAGrENT (annotation) reference archive (e.g. [VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz][ftp-ref]) +7. Subclonal reference archive ([SUBCL_ref_GRCh37d5.tar.gz][ftp-ref]) * Only needed if `skipbb` is `false` -1. QC reference archive (e.g. [qcGenotype_GRCh37d5.tar.gz][ftp-ref]) +8. QC reference archive (e.g. [qcGenotype_GRCh37d5.tar.gz][ftp-ref]) Inputs 1&2 are expected to have been mapped using [dockstore-cgpmap][dockstore-cgpmap] @@ -56,12 +52,12 @@ your own reference files. Much of this information is available on the individu pages (or the subsequently linked protocols papers). * Specific to dockstore-cgpwgs: - * [BRASS][brass-wiki] - * [ascatNgs][ascatngs-wiki] + * [BRASS][brass-wiki] + * [ascatNgs][ascatngs-wiki] * Inherited from dockstore-cgpwxs: - * [cgpCaVEManWrapper][caveman-wiki] - * [cgpPindel][cgppindel-wiki] - * [VAGrENT][vagrent-wiki] + * [cgpCaVEManWrapper][caveman-wiki] + * [cgpPindel][cgppindel-wiki] + * [VAGrENT][vagrent-wiki] ### Usable Cores @@ -80,7 +76,7 @@ If not set detects available cores on system. All of the tools installed as part of [dockstore-cgpmap][dockstore-cgpmap] and [dockstore-cgpwxs][dockstore-cgpwxs] and the above packages are available for direct use. -``` +```bash export CGPWGS_VER=X.X.X docker pull quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER docker --rm -ti [--volume ...] quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER bash @@ -90,13 +86,13 @@ docker --rm -ti [--volume ...] quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER bash The resulting docker container has been tested with Singularity. The command to exec is: -``` +```bash ds-cgpwgs.pl -h ``` Expected use would be along the lines of: -``` +```bash export CGPWGS_VER=X.X.X singularity pull docker://quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER @@ -125,9 +121,7 @@ via the options of `ds-cgpwgs.pl` ## Verifying your deployment The `examples/` tree contains test json files populated with data that can be used to verify the -tool. More details can be found [here](examples/README.md). - -The `expected/` tree contains the expected output for each tool. More details can be found [here](expected/README.md). +tool. ## Example data @@ -148,7 +142,7 @@ will need to be manually cleaned to keep the repo size down. Activate the hooks with -``` +```bash git config core.hooksPath git-hooks ``` @@ -157,21 +151,19 @@ git config core.hooksPath git-hooks This project is maintained using HubFlow. 1. Make appropriate changes -1. Build image locally -1. Run all example inputs and verify any changes are acceptable -1. Update `expected/` tree with output of last step -1. Bump version in `Dockerfile` and `Dockstore.cwl` -1. Push changes -1. Check state on Travis -1. Update expected datasets -1. Generate the release (add notes to GitHub) -1. Confirm that image has been built on [quay.io][quay-builds] -1. Update the [dockstore][dockstore-cgpwgs] entry, see [their docs][dockstore-get-started]. +2. Build image locally +3. Run all example inputs and verify any changes are acceptable +4. Bump version in `Dockerfile` and `Dockstore.cwl` +5. Push changes +6. Check state on Travis +7. Generate the release (add notes to GitHub) +8. Confirm that image has been built on [quay.io][quay-builds] +9. Update the [dockstore][dockstore-cgpwgs] entry, see [their docs][dockstore-get-started]. ## LICENCE -``` -Copyright (c) 2017-2018 Genome Research Ltd. +```none +Copyright (c) 2017-2019 Genome Research Ltd. Author: Cancer Genome Project From 0eb7ceeaede75e79e0620d7ae012153c23c4d883 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 8 Apr 2019 15:43:55 +0000 Subject: [PATCH 06/32] Bump base image --- Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/Dockerfile b/Dockerfile index 3637e58..149193a 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.0.3 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.1 as builder USER root From 30c100891f9e36553bb1b491d5bfd3b13238c55c Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 8 Apr 2019 15:45:03 +0000 Subject: [PATCH 07/32] Remove references to battenberg --- .travis.yml | 1 - build/opt-build.sh | 22 ++-------------- build/rlib-build.R | 10 +------- cwls/cgpwgs.cwl | 14 ---------- examples/cgpwgs/bam_bai.json | 5 ---- examples/cgpwgs/bam_bai_pu_pl.json | 5 ---- scripts/analysisWGS.sh | 41 ------------------------------ scripts/ds-cgpwgs.pl | 14 ---------- 8 files changed, 3 insertions(+), 109 deletions(-) diff --git a/.travis.yml b/.travis.yml index bdfef3d..0a6dc01 100644 --- a/.travis.yml +++ b/.travis.yml @@ -44,4 +44,3 @@ script: - docker run -t --rm dockstore-cgpwgs alleleCounter --version - docker run -t --rm dockstore-cgpwgs brass.pl -version - docker run -t --rm dockstore-cgpwgs ascat.pl -version - - docker run -t --rm dockstore-cgpwgs battenberg.pl -version diff --git a/build/opt-build.sh b/build/opt-build.sh index 4c083d9..1d5e98f 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -9,7 +9,7 @@ fi set -u ### alleleCount -VER_ALLELECOUNT="v4.0.0" +VER_ALLELECOUNT="v4.0.1" ### cgpNgsQc VER_CGPNGSQC="v1.5.1" @@ -23,13 +23,10 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/A VER_GRASS="v2.1.1" ### BRASS -VER_BRASS="v6.1.2" +VER_BRASS="v6.2.0" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/fasta-v36.3.8g.tar.gz" -### cgpBattenberg -VER_CGPBB="v3.3.0" - if [ "$#" -lt "1" ] ; then echo "Please provide an installation path such as /opt/ICGC" exit 1 @@ -220,21 +217,6 @@ if [ ! -e $SETUP_DIR/BRASS.success ]; then touch $SETUP_DIR/BRASS.success fi -### cgpBattenberg -if [ ! -e $SETUP_DIR/cgpBB.success ]; then - curl -sSL --retry 10 https://github.com/cancerit/cgpBattenberg/archive/${VER_CGPBB}.tar.gz > distro.tar.gz - rm -rf distro/* - tar --strip-components 1 -C distro -xzf distro.tar.gz - cd distro/Rsupport - ./setupR.sh $INST_PATH - cd $SETUP_DIR/distro/perl - cpanm --no-interactive --notest --mirror http://cpan.metacpan.org --notest -l $INST_PATH --installdeps . - cpanm -v --no-interactive --mirror http://cpan.metacpan.org -l $INST_PATH . - cd $SETUP_DIR - rm -rf distro.* distro/* - touch $SETUP_DIR/cgpBB.success -fi - cd $HOME rm -rf $SETUP_DIR diff --git a/build/rlib-build.R b/build/rlib-build.R index dddaab1..65ecce7 100644 --- a/build/rlib-build.R +++ b/build/rlib-build.R @@ -28,12 +28,4 @@ ipak(c("poweRlaw")) ipak(c("zlibbioc")) ipak(c("RColorBrewer")) -# add for BB -ipak(c("stringi")) -ipak(c("readr")) -ipak(c("doParallel")) -ipak(c("ggplot2")) -ipak(c("gridExtra")) -ipak(c("gtools")) - -install_github("sb43/copynumber", ref="f1688edc154f1a0e3aacf7781090afe02882f623") +install_github("Irrationone/copynumber", ref="87d2663fe6b11c03cf6006b4ee9ed70450eacb5a") diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index 1c63db0..869b4d7 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -62,13 +62,6 @@ inputs: prefix: -cnv_sv separate: true - subcl: - type: File - doc: "Supporting files for allele counts used by Battenberg Subclonal CNV analysis" - inputBinding: - prefix: -subcl - separate: true - qcset: type: File doc: "Supporting files for QC tools" @@ -134,13 +127,6 @@ inputs: separate: true shellQuote: true - skipbb: - type: boolean? - doc: "Skip Battenberg allele counts" - inputBinding: - prefix: -skipbb - separate: true - skipqc: type: boolean? doc: "Disable genotype and verifyBamID steps" diff --git a/examples/cgpwgs/bam_bai.json b/examples/cgpwgs/bam_bai.json index fd60c16..89b58a8 100644 --- a/examples/cgpwgs/bam_bai.json +++ b/examples/cgpwgs/bam_bai.json @@ -15,10 +15,6 @@ "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/CNV_SV_ref_GRCh37d5_brass6+.tar.gz", "class": "File" }, - "subcl": { - "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", - "class": "File" - }, "qcset": { "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", "class": "File" @@ -43,7 +39,6 @@ "species": "human", "assembly": "GRCh37d5", "cavereads": 800000, - "skipbb": true, "result_archive": { "path": "/tmp/result_WGS.tar.gz", "class": "File" diff --git a/examples/cgpwgs/bam_bai_pu_pl.json b/examples/cgpwgs/bam_bai_pu_pl.json index 3cbe4ee..aa82ef4 100644 --- a/examples/cgpwgs/bam_bai_pu_pl.json +++ b/examples/cgpwgs/bam_bai_pu_pl.json @@ -15,10 +15,6 @@ "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/CNV_SV_ref_GRCh37d5_brass6+.tar.gz", "class": "File" }, - "subcl": { - "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/SUBCL_ref_GRCh37d5.tar.gz", - "class": "File" - }, "qcset": { "path": "http://ftp.sanger.ac.uk/pub/cancer/dockstore/human/qcGenotype_GRCh37d5.tar.gz", "class": "File" @@ -43,7 +39,6 @@ "species": "human", "assembly": "GRCh37d5", "cavereads": 800000, - "skipbb": true, "purity": 0.99, "ploidy": 2, "result_archive": { diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 51c264b..d9e4ff0 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -164,25 +164,6 @@ do_parallel[CaVEMan_setup]="caveman.pl \ -x $CONTIG_EXCLUDE \ -p setup" -echo -e "\t[Parallel block 1] BB splitlocifiles added..." -if [ ! -z ${SKIPBB+x} ]; then - do_parallel[splitlocifiles]="echo 'BB splitlocifiles count disabled by params'" -else - do_parallel[splitlocifiles]="battenberg.pl \ - -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/battenberg \ - -u $REF_BASE/battenberg/1000genomesloci \ - -e $REF_BASE/battenberg/impute/impute_info.txt \ - -c $REF_BASE/battenberg/probloci.txt \ - -r $REF_BASE/genome.fa.fai \ - -ig $REF_BASE/battenberg/ignore_contigs.txt \ - -ge XX \ - -tb $BAM_MT_TMP \ - -nb $BAM_WT_TMP \ - -p splitlocifiles \ - -nl 50 \ - -t $CPU" -fi - if [ ! -z ${SKIPQC+x} ]; then do_parallel[geno]="echo 'Genotype Check disabled by params'" do_parallel[verify_WT]="echo 'VerifyBam Normal disabled by params'" @@ -213,25 +194,6 @@ unset do_parallel declare -A do_parallel echo -e "\nSetting up Parallel block 2" -echo -e "\t[Parallel block 2] BB alleleCount added..." -if [ ! -z ${SKIPBB+x} ]; then - do_parallel[alleleCount]="echo 'BB allele count disabled by params'" -else - do_parallel[alleleCount]="battenberg.pl \ - -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/battenberg \ - -u $REF_BASE/battenberg/1000genomesloci \ - -e $REF_BASE/battenberg/impute/impute_info.txt \ - -c $REF_BASE/battenberg/probloci.txt \ - -r $REF_BASE/genome.fa.fai \ - -ig $REF_BASE/battenberg/ignore_contigs.txt \ - -ge XX \ - -tb $BAM_MT_TMP \ - -nb $BAM_WT_TMP \ - -p allelecount \ - -nl 50 \ - -t $CPU" -fi - echo -e "\t[Parallel block 2] CaVEMan split added..." do_parallel[CaVEMan_split]="caveman.pl \ -r $REF_BASE/genome.fa.fai \ @@ -464,9 +426,6 @@ run_parallel do_parallel # clean up log files rm -rf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/*/logs -# cleanup battenberg logs -rm -f $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/battenberg/tmpBattenberg/logs/* - # correct default filenames from contamination jobs mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_WT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_WT}.time.verify_WT mv $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}.time.verify_MT $OUTPUT_DIR/timings/${PROTOCOL}_${NAME_MT}.time.verify_MT diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 96572ba..ab70bd9 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -28,7 +28,6 @@ 'a|annot=s' => \$opts{'a'}, 'si|snv_indel=s' => \$opts{'si'}, 'cs|cnv_sv=s' => \$opts{'cs'}, - 'sc|subcl=s' => \$opts{'sc'}, 'qc|qcset=s' => \$opts{'qc'}, 't|tumour=s' => \$opts{'t'}, 'tidx=s' => \$opts{'tidx'}, @@ -37,7 +36,6 @@ 'e|exclude=s' => \$opts{'e'}, 'sp|species:s' => \$opts{'sp'}, 'as|assembly:s' => \$opts{'as'}, - 'sb|skipbb' => \$opts{'sb'}, 'sq|skipqc' => \$opts{'sq'}, 'cr|cavereads:i' => \$opts{'cr'}, 'pc|pindelcpu:i' => \$opts{'pc'}, @@ -96,7 +94,6 @@ && $opts{'r'} eq $opts{'si'} && $opts{'r'} eq $opts{'cs'} && $opts{'r'} eq $opts{'qc'} - && (exists $opts{'sb'} || $opts{'r'} eq $opts{'sc'}) && -d $opts{'r'}) { $ref_area = $opts{'r'}; $ref_unpack = 0; @@ -107,7 +104,6 @@ ref_unpack($ref_area, $opts{'si'}); ref_unpack($ref_area, $opts{'cs'}); ref_unpack($ref_area, $opts{'qc'}); - ref_unpack($ref_area, $opts{'sc'}) if(! exists $opts{'sb'}); } ## now complete the caveman flaging file correctly @@ -279,7 +275,6 @@ =head1 SYNOPSIS -annot -a Path to VAGrENT*.tar.gz -snv_indel -si Path to SNV_INDEL*.tar.gz -cnv_sv -cs Path to CNV_SV*.tar.gz - -subcl -sc Path to SUBCL*.tar.gz -tumour -t Tumour [CR|B]AM file -tidx Tumour [CR|B]AM index (bai|csi|crai) -normal -n Normal [CR|B]AM file @@ -290,7 +285,6 @@ =head1 SYNOPSIS Optional parameters -species -sp Species name (may require quoting) -assembly -as Reference assembly - -skipbb -sb Skip Battenberg allele counts -pindelcpu -pc Max CPUs for pindel analysis, >8 ignored [8] -outdir -o Set the output folder [$HOME] -cores -c Set the number of cpu/cores available [default all]. @@ -326,10 +320,6 @@ =head1 OPTION DETAILS Path to Path to CNV_SV*.tar.gz -=item B<-subcl> - -Path to Path to SUBCL*.tar.gz - =item B<-tumour> Path to tumour BAM or CRAM file with co-located index and BAS file. @@ -352,10 +342,6 @@ =head1 OPTION DETAILS Specify overriding assembly, by default will select the most prevelant entry in [CR|B]AM header (to cope with inclusion of viral/decoy sequences). -=item B<-skipbb> - -Disables the Battenberg allele count generation - =item B<-skipqc> Disable genotype, gender and verifyBamID. Generally you want to set this for non-human data From e2eb73f1ab8424f1b44594c8dd6dd5d86a08f744 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 8 Apr 2019 15:57:31 +0000 Subject: [PATCH 08/32] version name convention for BRASS flipped again --- build/opt-build.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 1d5e98f..46ee6cc 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -23,7 +23,7 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/A VER_GRASS="v2.1.1" ### BRASS -VER_BRASS="v6.2.0" +VER_BRASS="6.2.0" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/fasta-v36.3.8g.tar.gz" From f83ee6c0c68b191bdd410020c1d88d2a1e2a9582 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 8 Apr 2019 15:57:59 +0000 Subject: [PATCH 09/32] Remove script checking so resume is available --- scripts/ds-cgpwgs.pl | 1 - 1 file changed, 1 deletion(-) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index ab70bd9..94834f9 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -112,7 +112,6 @@ my $run_file = $opts{'o'}.'/run.params'; open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; # Force explicit checking of file flush -print $FH "export PCAP_THREADED_NO_SCRIPT=1\n"; print $FH "export PCAP_THREADED_FORCE_SYNC=1\n"; print $FH "export PCAP_THREADED_LOADBACKOFF=1\n"; print $FH "export PCAP_THREADED_REM_LOGS=1\n"; From 58d96a679c8423ef0bbfe3c9542fcb64f2ff5dca Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 8 Apr 2019 15:58:43 +0000 Subject: [PATCH 10/32] Strip ENA from REF_PATH --- scripts/analysisWGS.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index d9e4ff0..701c492 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -137,7 +137,7 @@ if [ "$ALN_EXTN" == "cram" ]; then ## prime the cache USER_CACHE=$OUTPUT_DIR/ref_cache export REF_CACHE=$USER_CACHE/%2s/%2s/%s - export REF_PATH=$REF_CACHE:http://www.ebi.ac.uk/ena/cram/md5/%s + export REF_PATH=$REF_CACHE do_parallel[cache_POP]="seq_cache_populate.pl -root $USER_CACHE $REF_BASE/genome.fa" fi From 61cdb2f25eb581977a5ca436954a9dce8b146547 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 10 Apr 2019 07:53:14 +0000 Subject: [PATCH 11/32] Fix doc errors in cmdln help --- scripts/ds-cgpwgs.pl | 23 +++++++++++++++++------ 1 file changed, 17 insertions(+), 6 deletions(-) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index 94834f9..ec7b3ce 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -263,17 +263,18 @@ sub ref_unpack { =head1 NAME -dh-wrapper.pl - Generate the param file and execute analysisWGS.sh (for dockstore) +ds-cgpwgs.pl - Generate the param file and execute analysisWGS.sh (for dockstore) =head1 SYNOPSIS -dh-wrapper.pl [options] [file(s)...] +ds-cgpwgs.pl [options] [file(s)...] Required parameters: -reference -r Path to core reference tar.gz -annot -a Path to VAGrENT*.tar.gz -snv_indel -si Path to SNV_INDEL*.tar.gz -cnv_sv -cs Path to CNV_SV*.tar.gz + -qcset -qc Path to qcGenotype*.tar.gz -tumour -t Tumour [CR|B]AM file -tidx Tumour [CR|B]AM index (bai|csi|crai) -normal -n Normal [CR|B]AM file @@ -305,19 +306,29 @@ =head1 OPTION DETAILS =item B<-reference> -Path to mapping tar.gz reference files +Path to mapping tar.gz reference bundle or root of all expanded reference bundles. + +e.g. for each ref bundle needed + +mkdir expanded +curl -L ftp://ftp.sanger.ac.uk/pub/cancer/dockstore/.../core_ref_xxx.tar.gz \ +tar -C expanded --strip-components 1 -zx =item B<-annot> -Path to VAGrENT*.tar.gz +Path to VAGrENT*.tar.gz reference bundle or root of all expanded reference bundles. =item B<-snv_indel> -Path to Path to SNV_INDEL*.tar.gz +Path to SNV_INDEL*.tar.gz reference bundle or root of all expanded reference bundles. =item B<-cnv_sv> -Path to Path to CNV_SV*.tar.gz +Path to CNV_SV*.tar.gz reference bundle or root of all expanded reference bundles. + +=item B<-qcset> + +Path to qcGenotype*.tar.gz reference bundle or root of all expanded reference bundles. =item B<-tumour> From d929ef92efdb6fe67dbfc9a4c314905ee3f4759f Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 10 Apr 2019 07:56:48 +0000 Subject: [PATCH 12/32] Experiment to fix sync problem --- Dockerfile | 3 + Threaded.pm | 478 ++++++++++++++++++++++++++++++++++++++++++++++++++++ 2 files changed, 481 insertions(+) create mode 100644 Threaded.pm diff --git a/Dockerfile b/Dockerfile index 149193a..c7e547e 100644 --- a/Dockerfile +++ b/Dockerfile @@ -108,6 +108,9 @@ COPY scripts/analysisWGS.sh $OPT/bin/analysisWGS.sh COPY scripts/ds-cgpwgs.pl $OPT/bin/ds-cgpwgs.pl RUN chmod a+x $OPT/bin/analysisWGS.sh $OPT/bin/ds-cgpwgs.pl +## HACK +cp Threaded.pm $OPT/lib/perl5/PCAP/Threaded.pm + ## USER CONFIGURATION RUN adduser --disabled-password --gecos '' ubuntu && chsh -s /bin/bash && mkdir -p /home/ubuntu diff --git a/Threaded.pm b/Threaded.pm new file mode 100644 index 0000000..bd4a84d --- /dev/null +++ b/Threaded.pm @@ -0,0 +1,478 @@ +package PCAP::Threaded; + +##########LICENCE########## +# PCAP - NGS reference implementations and helper code for the ICGC/TCGA Pan-Cancer Analysis Project +# Copyright (C) 2014-2018 ICGC PanCancer Project +# Copyright (C) 2018-2019 Cancer, Ageing and Somatic Mutation, Genome Research Limited +# +# This program is free software; you can redistribute it and/or +# modify it under the terms of the GNU General Public License +# as published by the Free Software Foundation; either version 2 +# of the License, or (at your option) any later version. +# +# This program is distributed in the hope that it will be useful, +# but WITHOUT ANY WARRANTY; without even the implied warranty of +# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the +# GNU General Public License for more details. +# +# You should have received a copy of the GNU General Public License +# along with this program; if not see: +# http://www.gnu.org/licenses/gpl-2.0.html +##########LICENCE########## + + +use PCAP; + +use strict; +use autodie qw(:all); +use English qw( -no_match_vars ); +use warnings FATAL => 'all'; +use Carp qw( croak ); +use File::Spec; +use File::Path qw(make_path); +use Try::Tiny qw(try catch finally); +use Capture::Tiny qw(capture); +use Const::Fast qw(const); +use Scalar::Util qw(looks_like_number); +use Time::HiRes qw(usleep); + +our $CAN_USE_THREADS = eval 'use threads; 1' || 0; + +const my $SCRIPT_OCT_MODE => 0777; + +our $OUT_ERR = 1; + +sub new { + my ($class, $max_threads) = @_; + croak "Number of threads was NAN: $max_threads" if(defined $max_threads && !looks_like_number($max_threads)); + unless(defined $max_threads) { + warn "Thread count not defined, defaulting to 1.\n"; + $max_threads = 1; + } + if($max_threads > 1 && $CAN_USE_THREADS == 0) { + warn "Threading is not available perl component will run as a single process"; + $max_threads = 1; + } + + my $self = {'threads' => $max_threads, + 'join_interval' => 1,}; + bless $self, $class; + + # get core count for optional auto-back off when oversubscribed + $self->init_cores(); + + return $self; +} + +sub disable_out_err { + $OUT_ERR = 0; + return $OUT_ERR; +} + +sub enable_out_err { + $OUT_ERR = 1; + return $OUT_ERR; +} + +sub use_out_err { + return $OUT_ERR; +} + +sub init_cores { + my $self = shift; + if($ENV{PCAP_THREADED_LOADBACKOFF}) { + my ($cpus, $se, $ex) = capture { system('grep -c ^processor /proc/cpuinfo'); }; + chomp $cpus; + $self->{'system_cpus'} = $cpus; + } + return 1; +} + +sub need_backoff { + my $self = shift; + my $ret = 0; + # don't want to change normal behaviour + if($ENV{PCAP_THREADED_LOADBACKOFF}) { + my($uptime, $se, $ex) = capture { system('uptime'); }; + chomp $uptime; + # probably only need 1-min but grab them all + my ($one_min, $five_min, $fifteen_min) = $uptime =~ m/load average: ([[:digit:]]+\.[[:digit:]]+), ([[:digit:]]+\.[[:digit:]]+), ([[:digit:]]+\.[[:digit:]]+)$/; + $ret = 1 if($one_min > $self->{'system_cpus'}); + } + return $ret; +} + +sub add_function { + my ($self, $function_name, $function_ref, $divisor) = @_; + croak "Function $function_name has already been defined.\n" if(exists $self->{'functions'}->{$function_name}->{'code'}); + my $ref_type = ref $function_ref; + $ref_type ||= 'not a reference'; + croak "Second argument to add_function should be a code reference, I got '$ref_type'.\n" unless('CODE' eq ref $function_ref); + + $self->{'functions'}->{$function_name}->{'code'} = $function_ref; + $self->{'functions'}->{$function_name}->{'threads'} = $self->_suitable_threads($divisor); + + return 1; +} + +sub thread_join_interval { + my ($self, $sec) = @_; + if(defined $sec) { + croak 'join_interval must be an integer' if($sec !~ m/^[[:digit:]]+$/); + croak 'join_interval must be 1 or more' if($sec < 1); + $self->{'join_interval'} = $sec; + } + $self->{'join_interval'}; +} + +sub run { + my ($self, $iterations, $function_name, @params) = @_; + croak 'Iterations must be defined' unless(defined $iterations); + croak "Iterations must be a positive integer: $iterations" if($iterations !~ m/^[[:digit:]]+$/ || $iterations == 0); + croak 'Function_name must be defined' unless(defined $function_name); + croak "Unable to find '$function_name', please check your declaration of add_function()\n." + unless(exists $self->{'functions'}->{$function_name}->{'code'}); + + my $function_ref = $self->{'functions'}->{$function_name}->{'code'}; + my $thread_count = $self->{'functions'}->{$function_name}->{'threads'}; + + my $start_interval = 6; # initial scaling + if($self->{'threads'} >= 8) { + # when core count is higher spread the scaling up over 2 minutes + $start_interval = int 120 / $self->{'threads'}; + $start_interval = 6 if($start_interval < 6); + } + + # uncoverable branch true + if($thread_count > 1 && $CAN_USE_THREADS) { + # reserve 0 for when people want to use 'success_exists/touch_success' for non-threaded steps + # makes it easy to see in progress area which steps are threaded + my $index = 1; + while($index <= $iterations) { + while(threads->list(threads::all()) < $thread_count && $index <= $iterations) { + while($self->need_backoff) { + warn "Excessive load average, take a break... have a Kit-Kat!\n"; + sleep 30; + } + threads->create($function_ref, $index++, @params); + last if($index > $iterations); + usleep($start_interval * 1_000_000); + } + $start_interval = 0.1; # once the initial scale up is complete start interval can be tiny. + sleep $self->thread_join_interval while(threads->list(threads::joinable()) == 0); + for my $thr(threads->list(threads::joinable())) { + $thr->join; + if(my $err = $thr->error) { die "Thread error: $err\n"; } + } + } + # last gasp for any remaining threads + sleep $self->thread_join_interval while(threads->list(threads::running()) > 0); + for my $thr(threads->list(threads::joinable())) { + $thr->join; + if(my $err = $thr->error) { die "Thread error: $err\n"; } + } + } + else { + for my $index(1..$iterations) { + &{$function_ref}($index, @params); + } + } + return 1; +} + +sub _suitable_threads { + my ($self, $divisor) = @_; + my $suitable_threads = $self->{'threads'}; + if(defined $divisor) { + croak "Thread divisior must be a positive integer: $divisor\n." if($divisor !~ m/^[[:digit:]]+$/xms || $divisor == 0); + $suitable_threads = int ($suitable_threads / $divisor); + $suitable_threads++ if($suitable_threads == 0); + } + return $suitable_threads; +} + +sub success_exists { + my ($tmp, @indexes) = @_; + my ($legacy_type) = (caller(1))[3]; + my $new_type = $legacy_type; + $new_type =~ s/::/_/g; + + my $suffix = join '.', @indexes; + for my $type($new_type, $legacy_type) { # we should test the new file name style first + my $file = $type.'.'.$suffix; + my $path = File::Spec->catfile($tmp, $file); + if(-e $path) { + warn "Skipping $file as previously successful\n"; + return 1; + } + } + return 0; +} + +sub touch_success { + my ($tmp, @indexes) = @_; + my ($type) = (caller(1))[3]; + $type =~ s/::/_/g; + make_path($tmp) unless(-d $tmp); + my $file = join '.', $type, @indexes; + my $path = File::Spec->catfile($tmp, $file); + open my $TOUCH, '>', $path; + close $TOUCH; + return 1; +} + +sub _legacy_touch_success { + my ($tmp, @indexes) = @_; + my ($type) = (caller(1))[3]; + make_path($tmp) unless(-d $tmp); + my $file = join '.', $type, @indexes; + my $path = File::Spec->catfile($tmp, $file); + open my $TOUCH, '>', $path; + close $TOUCH; + return 1; +} + +sub external_process_handler { + my ($tmp, $command_in, @indexes) = @_; + + my @commands; + if(ref $command_in eq 'ARRAY') { + @commands = @{$command_in} + } + else { + @commands = ($command_in); + } + + if(&use_out_err == 0) { + # these may be marshalled to different files so output both + try { + for my $c(@commands) { + warn "\nErrors from command: $c\n\n"; + print "\nOutput from command: $c\n\n"; + system($c); + } + } + catch { die $_; }; + } + else { + my $caller = (caller(1))[3]; + $caller =~ s/::/_/g; + my $suffix = join q{.}, @indexes; + + my $script = _create_script(\@commands, File::Spec->catfile($tmp, "$caller.$suffix")); + + my $out = File::Spec->catfile($tmp, "$caller.$suffix.out"); + my $err = File::Spec->catfile($tmp, "$caller.$suffix.err"); + + try { + system("/usr/bin/time bash $script 1> $out 2> $err"); + } + catch { + warn "\nGeneral output can be found in this file: $out\n"; + warn "Errors can be found in this file: $err\n\n"; + die "Wrapper script message:\n".$_; + }; + + unlink $script; # only leave scripts if we fail + if($ENV{PCAP_THREADED_REM_LOGS}) { + unlink $err; + unlink $out; + } + } + + return 1; +} + +sub _create_script { + my ($commands, $stub) = @_; + + my $script = "$stub.sh"; + + if($ENV{PCAP_THREADED_NO_SCRIPT} && -e $script) { + die "ERROR: Script already present, delete to proceed: $script"; + } + + open my $SH, '>', $script or die "Failed to create $script"; + print $SH qq{#!/bin/bash\nset -eux\n} or die "Write to $script failed"; + print $SH join qq{\n}, @{$commands}, q{} or die "Write to $script failed"; + close $SH; + + my $microsec = 100_000; + _file_complete($script, $microsec); + return $script; +} + +sub _file_complete { + my ($script, $microsec) = @_; + usleep($microsec); + my $tries = 0; + while(! -e $script) { + $tries++; + croak "Failed to find script after 30 attempts ($microsec us delays): $script" if($tries >= 30); + usleep($microsec); + } + $tries = 0; + while(1) { + $tries++; + croak "Failed to confirm write complete after 30 attempts ($microsec us delays): $script" if($tries >= 30); + my ($stdout, $stderr, $exit) = capture { system([0,1], 'fuser', $script); }; + if($exit > 1) { + croak sprintf "ERROR: fuser output\n\tSTDOUT: %s\n\tSTDERR: %s\n\tEXIT: %d\n", $stdout, $stderr, $exit; + } + printf STDERR "OUT : %s\nERR : %s\nEXIT: %s\n", $stdout,$stderr,$exit if($exit == 0); + last if($exit == 1); + usleep($microsec); + } + return 1; +} + +1; + +__END__ + +=head1 NAME + +PCAP::Threaded - Run threaded processing easily. + +=head2 Constructor + +=over 4 + +=item PCAP::Threaded->new(max_threads) + +Generate a threaded processing object with the defined number of threads. + +=back + +=head2 Methods + +=over 4 + +=item add_function + +Register a named coderef to be run using threads. + + $threads->add_function($function_name, $coderef [, $divisor]); + + function_name - Text to address function by in L. + coderef - Reference to subroutine. + divisor - See L<_suitable_threads()|PCAP::Threaded/_suitable_threads>. + +=item run + +Run the named function for the stated number of iterations. + +NOTE: If your process needs to honor selected index processing this needs to be handled in the +callback registered in add_function. + + $threads->run($iterations, $function_name, @params); + + iterations - Number of times the function should be started iteration + number (origin 1) is passed as first argument to the code-ref + defined in L. + function_name - Name of function as defined in L. + @params - Any additional params for the coderef. + +=back + +=head2 Utility Methods + +These are non-object methods which are useful to related code + +=head3 Configuration + +=over 4 + +=item use_out_err + +Determines if stdout/stderr are redirected to file, by default 1/true. + +=item disable_out_err + +Prevent calls to external_process_handler from redirecting stdout/stderr to files. + +=item enable_out_err + +Enable redirect of stdout/stderr to files when calling external_process_handler. + +=item thread_join_interval + +Set/get the number of seconds to wait between thread joins. Default 1. + +=back + +=head3 Resume Helpers + +These are useful methods to help you program resume from the last successfully completed step. + +It is recommended that you use these in any callbacks used in the +L function to prevent unnecessary processing after restarting +a job. + +These can also be used for other processes in your program flow that aren't using threads. Where +these don't require an incrementing index value please use '0' (zero); + +=over 4 + +=item success_exists + +B compute step add this code: + + return if PCAP::Threaded::success_exists($tmpdir, $index[, $index_2...]); + +Requires implementation of L. + +($index_2... may be useful for some other implementation, see L). + +=item touch_success + +B compute step add this code: + + return if PCAP::Threaded::touch_success($tmpdir, $index[, $index_2...]); + +Requires implementation of L. + +($index_2... may be useful for some other implementation, see L). + +=item external_process_handler + + PCAP::Threaded::external_process_handler($logdir, $commands, $index[, $index_2...]); + + @params logdir - Path to pre-existing log directory + @params commands - Scalar command or arr-ref of commands + @params index - Which index this is, specifically for log/err files. + +Wraps up command with stdout and stderr catchalls to keep the output of each threaded process +separated from the script itself. Added to simplify interpretation of any issues that may occur. + +If you don't want to capture stdout/stderr see . + +($index_2... may be useful for some other implementation, see L). + +=back + +=head2 Internal functions + +=over 4 + +=item _suitable_threads + + $self->_suitable_threads($divisor); + +If the code-ref to be executed uses multiple threads (or piped processes) the total number of +parallel jobs will be divided by this number to prevent over subscription of resources. Take this +simple example: + + grep -wF 'wibble' | cut -f 5 | sort | uniq -c + +This 'pipeline' can theoretically use 4 CPU's at 100% each. When executing multiple parallel +executions of this the total number of threads defined on the command line is divided by the +provided value: + + max_parallel = int(total_threads / divisor); + max_parallel++ if(max_parallel == 0); + +If total_threads is < divisor a single process will run, any prevention of oversubscription then +falls to the OS and any CPU affinity settings. + +=back From 7da88c1e64beb0a746c23bbcd0a16eab3384e755 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 10 Apr 2019 08:02:04 +0000 Subject: [PATCH 13/32] docker syntax needed --- Dockerfile | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/Dockerfile b/Dockerfile index c7e547e..27a1a1b 100644 --- a/Dockerfile +++ b/Dockerfile @@ -109,7 +109,7 @@ COPY scripts/ds-cgpwgs.pl $OPT/bin/ds-cgpwgs.pl RUN chmod a+x $OPT/bin/analysisWGS.sh $OPT/bin/ds-cgpwgs.pl ## HACK -cp Threaded.pm $OPT/lib/perl5/PCAP/Threaded.pm +COPY Threaded.pm $OPT/lib/perl5/PCAP/Threaded.pm ## USER CONFIGURATION RUN adduser --disabled-password --gecos '' ubuntu && chsh -s /bin/bash && mkdir -p /home/ubuntu From db2513a0aca67c88cf83058f02754c5ecae5e7d0 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 16 Apr 2019 11:49:13 +0000 Subject: [PATCH 14/32] Layer minimisation and removal of test of Threaded.pm change --- Dockerfile | 70 ++++---- Threaded.pm | 478 ---------------------------------------------------- 2 files changed, 35 insertions(+), 513 deletions(-) delete mode 100644 Threaded.pm diff --git a/Dockerfile b/Dockerfile index 27a1a1b..9e9d975 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.1 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.2 as builder USER root @@ -60,37 +60,40 @@ LABEL vendor="Cancer Genome Project, Wellcome Trust Sanger Institute" LABEL uk.ac.sanger.cgp.description="CGP WGS pipeline for dockstore.org" LABEL uk.ac.sanger.cgp.version="2.0.2" -RUN bash -c 'apt-get update -yq >& this.log || (cat this.log 1>&2 && exit 1)' -RUN bash -c 'apt-get install -qy --no-install-recommends lsb-release >& this.log || (cat this.log 1>&2 && exit 1)' - -RUN echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list -RUN gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 -RUN gpg -a --export E084DAB9 | apt-key add - - -RUN bash -c 'apt-get update -yq >& this.log || (cat this.log 1>&2 && exit 1)' -RUN bash -c 'apt-get install -yq --no-install-recommends\ - apt-transport-https\ - locales\ - curl\ - ca-certificates\ - libperlio-gzip-perl\ - libssh2-1\ - bzip2\ - psmisc\ - time\ - zlib1g\ - liblzma5\ - libncurses5\ - libcairo2\ - gfortran\ - r-base\ - exonerate\ - libboost-iostreams-dev\ - p11-kit\ - >& this.log || (cat this.log 1>&2 && exit 1)' - -RUN bash -c 'locale-gen en_US.UTF-8 >& this.log || (cat this.log 1>&2 && exit 1)' -RUN bash -c 'update-locale LANG=en_US.UTF-8 >& this.log || (cat this.log 1>&2 && exit 1)' +RUN apt-get update -yq && \ +apt-get install -qy --no-install-recommends lsb-release + +RUN echo "deb http://cran.rstudio.com/bin/linux/ubuntu `lsb_release -cs`/" >> /etc/apt/sources.list && \ +gpg --keyserver keyserver.ubuntu.com --recv-key E084DAB9 && \ +gpg -a --export E084DAB9 | apt-key add - + +RUN apt-get update -yq && \ +apt-get install -yq --no-install-recommends \ +apt-transport-https \ +locales \ +curl \ +ca-certificates \ +libperlio-gzip-perl \ +libssh2-1 \ +bzip2 \ +psmisc \ +time \ +zlib1g \ +liblzma5 \ +libncurses5 \ +libcairo2 \ +gfortran \ +r-base \ +exonerate \ +libboost-iostreams-dev \ +p11-kit \ +unattended-upgrades && \ +unattended-upgrade -d -v && \ +apt-get remove -yq unattended-upgrades && \ +apt-get autoremove -yq + +RUN locale-gen en_US.UTF-8 && \ +update-locale LANG=en_US.UTF-8 ENV OPT /opt/wtsi-cgp ENV PATH $OPT/bin:$OPT/biobambam2/bin:$PATH @@ -108,9 +111,6 @@ COPY scripts/analysisWGS.sh $OPT/bin/analysisWGS.sh COPY scripts/ds-cgpwgs.pl $OPT/bin/ds-cgpwgs.pl RUN chmod a+x $OPT/bin/analysisWGS.sh $OPT/bin/ds-cgpwgs.pl -## HACK -COPY Threaded.pm $OPT/lib/perl5/PCAP/Threaded.pm - ## USER CONFIGURATION RUN adduser --disabled-password --gecos '' ubuntu && chsh -s /bin/bash && mkdir -p /home/ubuntu diff --git a/Threaded.pm b/Threaded.pm deleted file mode 100644 index bd4a84d..0000000 --- a/Threaded.pm +++ /dev/null @@ -1,478 +0,0 @@ -package PCAP::Threaded; - -##########LICENCE########## -# PCAP - NGS reference implementations and helper code for the ICGC/TCGA Pan-Cancer Analysis Project -# Copyright (C) 2014-2018 ICGC PanCancer Project -# Copyright (C) 2018-2019 Cancer, Ageing and Somatic Mutation, Genome Research Limited -# -# This program is free software; you can redistribute it and/or -# modify it under the terms of the GNU General Public License -# as published by the Free Software Foundation; either version 2 -# of the License, or (at your option) any later version. -# -# This program is distributed in the hope that it will be useful, -# but WITHOUT ANY WARRANTY; without even the implied warranty of -# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the -# GNU General Public License for more details. -# -# You should have received a copy of the GNU General Public License -# along with this program; if not see: -# http://www.gnu.org/licenses/gpl-2.0.html -##########LICENCE########## - - -use PCAP; - -use strict; -use autodie qw(:all); -use English qw( -no_match_vars ); -use warnings FATAL => 'all'; -use Carp qw( croak ); -use File::Spec; -use File::Path qw(make_path); -use Try::Tiny qw(try catch finally); -use Capture::Tiny qw(capture); -use Const::Fast qw(const); -use Scalar::Util qw(looks_like_number); -use Time::HiRes qw(usleep); - -our $CAN_USE_THREADS = eval 'use threads; 1' || 0; - -const my $SCRIPT_OCT_MODE => 0777; - -our $OUT_ERR = 1; - -sub new { - my ($class, $max_threads) = @_; - croak "Number of threads was NAN: $max_threads" if(defined $max_threads && !looks_like_number($max_threads)); - unless(defined $max_threads) { - warn "Thread count not defined, defaulting to 1.\n"; - $max_threads = 1; - } - if($max_threads > 1 && $CAN_USE_THREADS == 0) { - warn "Threading is not available perl component will run as a single process"; - $max_threads = 1; - } - - my $self = {'threads' => $max_threads, - 'join_interval' => 1,}; - bless $self, $class; - - # get core count for optional auto-back off when oversubscribed - $self->init_cores(); - - return $self; -} - -sub disable_out_err { - $OUT_ERR = 0; - return $OUT_ERR; -} - -sub enable_out_err { - $OUT_ERR = 1; - return $OUT_ERR; -} - -sub use_out_err { - return $OUT_ERR; -} - -sub init_cores { - my $self = shift; - if($ENV{PCAP_THREADED_LOADBACKOFF}) { - my ($cpus, $se, $ex) = capture { system('grep -c ^processor /proc/cpuinfo'); }; - chomp $cpus; - $self->{'system_cpus'} = $cpus; - } - return 1; -} - -sub need_backoff { - my $self = shift; - my $ret = 0; - # don't want to change normal behaviour - if($ENV{PCAP_THREADED_LOADBACKOFF}) { - my($uptime, $se, $ex) = capture { system('uptime'); }; - chomp $uptime; - # probably only need 1-min but grab them all - my ($one_min, $five_min, $fifteen_min) = $uptime =~ m/load average: ([[:digit:]]+\.[[:digit:]]+), ([[:digit:]]+\.[[:digit:]]+), ([[:digit:]]+\.[[:digit:]]+)$/; - $ret = 1 if($one_min > $self->{'system_cpus'}); - } - return $ret; -} - -sub add_function { - my ($self, $function_name, $function_ref, $divisor) = @_; - croak "Function $function_name has already been defined.\n" if(exists $self->{'functions'}->{$function_name}->{'code'}); - my $ref_type = ref $function_ref; - $ref_type ||= 'not a reference'; - croak "Second argument to add_function should be a code reference, I got '$ref_type'.\n" unless('CODE' eq ref $function_ref); - - $self->{'functions'}->{$function_name}->{'code'} = $function_ref; - $self->{'functions'}->{$function_name}->{'threads'} = $self->_suitable_threads($divisor); - - return 1; -} - -sub thread_join_interval { - my ($self, $sec) = @_; - if(defined $sec) { - croak 'join_interval must be an integer' if($sec !~ m/^[[:digit:]]+$/); - croak 'join_interval must be 1 or more' if($sec < 1); - $self->{'join_interval'} = $sec; - } - $self->{'join_interval'}; -} - -sub run { - my ($self, $iterations, $function_name, @params) = @_; - croak 'Iterations must be defined' unless(defined $iterations); - croak "Iterations must be a positive integer: $iterations" if($iterations !~ m/^[[:digit:]]+$/ || $iterations == 0); - croak 'Function_name must be defined' unless(defined $function_name); - croak "Unable to find '$function_name', please check your declaration of add_function()\n." - unless(exists $self->{'functions'}->{$function_name}->{'code'}); - - my $function_ref = $self->{'functions'}->{$function_name}->{'code'}; - my $thread_count = $self->{'functions'}->{$function_name}->{'threads'}; - - my $start_interval = 6; # initial scaling - if($self->{'threads'} >= 8) { - # when core count is higher spread the scaling up over 2 minutes - $start_interval = int 120 / $self->{'threads'}; - $start_interval = 6 if($start_interval < 6); - } - - # uncoverable branch true - if($thread_count > 1 && $CAN_USE_THREADS) { - # reserve 0 for when people want to use 'success_exists/touch_success' for non-threaded steps - # makes it easy to see in progress area which steps are threaded - my $index = 1; - while($index <= $iterations) { - while(threads->list(threads::all()) < $thread_count && $index <= $iterations) { - while($self->need_backoff) { - warn "Excessive load average, take a break... have a Kit-Kat!\n"; - sleep 30; - } - threads->create($function_ref, $index++, @params); - last if($index > $iterations); - usleep($start_interval * 1_000_000); - } - $start_interval = 0.1; # once the initial scale up is complete start interval can be tiny. - sleep $self->thread_join_interval while(threads->list(threads::joinable()) == 0); - for my $thr(threads->list(threads::joinable())) { - $thr->join; - if(my $err = $thr->error) { die "Thread error: $err\n"; } - } - } - # last gasp for any remaining threads - sleep $self->thread_join_interval while(threads->list(threads::running()) > 0); - for my $thr(threads->list(threads::joinable())) { - $thr->join; - if(my $err = $thr->error) { die "Thread error: $err\n"; } - } - } - else { - for my $index(1..$iterations) { - &{$function_ref}($index, @params); - } - } - return 1; -} - -sub _suitable_threads { - my ($self, $divisor) = @_; - my $suitable_threads = $self->{'threads'}; - if(defined $divisor) { - croak "Thread divisior must be a positive integer: $divisor\n." if($divisor !~ m/^[[:digit:]]+$/xms || $divisor == 0); - $suitable_threads = int ($suitable_threads / $divisor); - $suitable_threads++ if($suitable_threads == 0); - } - return $suitable_threads; -} - -sub success_exists { - my ($tmp, @indexes) = @_; - my ($legacy_type) = (caller(1))[3]; - my $new_type = $legacy_type; - $new_type =~ s/::/_/g; - - my $suffix = join '.', @indexes; - for my $type($new_type, $legacy_type) { # we should test the new file name style first - my $file = $type.'.'.$suffix; - my $path = File::Spec->catfile($tmp, $file); - if(-e $path) { - warn "Skipping $file as previously successful\n"; - return 1; - } - } - return 0; -} - -sub touch_success { - my ($tmp, @indexes) = @_; - my ($type) = (caller(1))[3]; - $type =~ s/::/_/g; - make_path($tmp) unless(-d $tmp); - my $file = join '.', $type, @indexes; - my $path = File::Spec->catfile($tmp, $file); - open my $TOUCH, '>', $path; - close $TOUCH; - return 1; -} - -sub _legacy_touch_success { - my ($tmp, @indexes) = @_; - my ($type) = (caller(1))[3]; - make_path($tmp) unless(-d $tmp); - my $file = join '.', $type, @indexes; - my $path = File::Spec->catfile($tmp, $file); - open my $TOUCH, '>', $path; - close $TOUCH; - return 1; -} - -sub external_process_handler { - my ($tmp, $command_in, @indexes) = @_; - - my @commands; - if(ref $command_in eq 'ARRAY') { - @commands = @{$command_in} - } - else { - @commands = ($command_in); - } - - if(&use_out_err == 0) { - # these may be marshalled to different files so output both - try { - for my $c(@commands) { - warn "\nErrors from command: $c\n\n"; - print "\nOutput from command: $c\n\n"; - system($c); - } - } - catch { die $_; }; - } - else { - my $caller = (caller(1))[3]; - $caller =~ s/::/_/g; - my $suffix = join q{.}, @indexes; - - my $script = _create_script(\@commands, File::Spec->catfile($tmp, "$caller.$suffix")); - - my $out = File::Spec->catfile($tmp, "$caller.$suffix.out"); - my $err = File::Spec->catfile($tmp, "$caller.$suffix.err"); - - try { - system("/usr/bin/time bash $script 1> $out 2> $err"); - } - catch { - warn "\nGeneral output can be found in this file: $out\n"; - warn "Errors can be found in this file: $err\n\n"; - die "Wrapper script message:\n".$_; - }; - - unlink $script; # only leave scripts if we fail - if($ENV{PCAP_THREADED_REM_LOGS}) { - unlink $err; - unlink $out; - } - } - - return 1; -} - -sub _create_script { - my ($commands, $stub) = @_; - - my $script = "$stub.sh"; - - if($ENV{PCAP_THREADED_NO_SCRIPT} && -e $script) { - die "ERROR: Script already present, delete to proceed: $script"; - } - - open my $SH, '>', $script or die "Failed to create $script"; - print $SH qq{#!/bin/bash\nset -eux\n} or die "Write to $script failed"; - print $SH join qq{\n}, @{$commands}, q{} or die "Write to $script failed"; - close $SH; - - my $microsec = 100_000; - _file_complete($script, $microsec); - return $script; -} - -sub _file_complete { - my ($script, $microsec) = @_; - usleep($microsec); - my $tries = 0; - while(! -e $script) { - $tries++; - croak "Failed to find script after 30 attempts ($microsec us delays): $script" if($tries >= 30); - usleep($microsec); - } - $tries = 0; - while(1) { - $tries++; - croak "Failed to confirm write complete after 30 attempts ($microsec us delays): $script" if($tries >= 30); - my ($stdout, $stderr, $exit) = capture { system([0,1], 'fuser', $script); }; - if($exit > 1) { - croak sprintf "ERROR: fuser output\n\tSTDOUT: %s\n\tSTDERR: %s\n\tEXIT: %d\n", $stdout, $stderr, $exit; - } - printf STDERR "OUT : %s\nERR : %s\nEXIT: %s\n", $stdout,$stderr,$exit if($exit == 0); - last if($exit == 1); - usleep($microsec); - } - return 1; -} - -1; - -__END__ - -=head1 NAME - -PCAP::Threaded - Run threaded processing easily. - -=head2 Constructor - -=over 4 - -=item PCAP::Threaded->new(max_threads) - -Generate a threaded processing object with the defined number of threads. - -=back - -=head2 Methods - -=over 4 - -=item add_function - -Register a named coderef to be run using threads. - - $threads->add_function($function_name, $coderef [, $divisor]); - - function_name - Text to address function by in L. - coderef - Reference to subroutine. - divisor - See L<_suitable_threads()|PCAP::Threaded/_suitable_threads>. - -=item run - -Run the named function for the stated number of iterations. - -NOTE: If your process needs to honor selected index processing this needs to be handled in the -callback registered in add_function. - - $threads->run($iterations, $function_name, @params); - - iterations - Number of times the function should be started iteration - number (origin 1) is passed as first argument to the code-ref - defined in L. - function_name - Name of function as defined in L. - @params - Any additional params for the coderef. - -=back - -=head2 Utility Methods - -These are non-object methods which are useful to related code - -=head3 Configuration - -=over 4 - -=item use_out_err - -Determines if stdout/stderr are redirected to file, by default 1/true. - -=item disable_out_err - -Prevent calls to external_process_handler from redirecting stdout/stderr to files. - -=item enable_out_err - -Enable redirect of stdout/stderr to files when calling external_process_handler. - -=item thread_join_interval - -Set/get the number of seconds to wait between thread joins. Default 1. - -=back - -=head3 Resume Helpers - -These are useful methods to help you program resume from the last successfully completed step. - -It is recommended that you use these in any callbacks used in the -L function to prevent unnecessary processing after restarting -a job. - -These can also be used for other processes in your program flow that aren't using threads. Where -these don't require an incrementing index value please use '0' (zero); - -=over 4 - -=item success_exists - -B compute step add this code: - - return if PCAP::Threaded::success_exists($tmpdir, $index[, $index_2...]); - -Requires implementation of L. - -($index_2... may be useful for some other implementation, see L). - -=item touch_success - -B compute step add this code: - - return if PCAP::Threaded::touch_success($tmpdir, $index[, $index_2...]); - -Requires implementation of L. - -($index_2... may be useful for some other implementation, see L). - -=item external_process_handler - - PCAP::Threaded::external_process_handler($logdir, $commands, $index[, $index_2...]); - - @params logdir - Path to pre-existing log directory - @params commands - Scalar command or arr-ref of commands - @params index - Which index this is, specifically for log/err files. - -Wraps up command with stdout and stderr catchalls to keep the output of each threaded process -separated from the script itself. Added to simplify interpretation of any issues that may occur. - -If you don't want to capture stdout/stderr see . - -($index_2... may be useful for some other implementation, see L). - -=back - -=head2 Internal functions - -=over 4 - -=item _suitable_threads - - $self->_suitable_threads($divisor); - -If the code-ref to be executed uses multiple threads (or piped processes) the total number of -parallel jobs will be divided by this number to prevent over subscription of resources. Take this -simple example: - - grep -wF 'wibble' | cut -f 5 | sort | uniq -c - -This 'pipeline' can theoretically use 4 CPU's at 100% each. When executing multiple parallel -executions of this the total number of threads defined on the command line is divided by the -provided value: - - max_parallel = int(total_threads / divisor); - max_parallel++ if(max_parallel == 0); - -If total_threads is < divisor a single process will run, any prevention of oversubscription then -falls to the OS and any CPU affinity settings. - -=back From 723225280d3c70861233847ffe9d6973a5b88612 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 16 Apr 2019 18:30:45 +0000 Subject: [PATCH 15/32] more explicit --- .dockerignore | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/.dockerignore b/.dockerignore index a637a6b..911ae83 100644 --- a/.dockerignore +++ b/.dockerignore @@ -2,5 +2,6 @@ /examples/ /images/ /git-hooks/ -/*.* +/CHANGES.md +/README.md /LICENSE From c007ab2dce5d966777a99c67314479ed4ba946ee Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 16 Apr 2019 18:31:21 +0000 Subject: [PATCH 16/32] Remove dumping of env to log files --- scripts/analysisWGS.sh | 1 - 1 file changed, 1 deletion(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 701c492..18b2f94 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -61,7 +61,6 @@ if [ ! -f $PARAM_FILE ]; then exit 1 fi source $PARAM_FILE -env if [ -z ${CPU+x} ]; then CPU=`grep -c ^processor /proc/cpuinfo` From 06692e2dd892d7306e373d708684c14f1cfc9cb8 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 16 Apr 2019 18:31:47 +0000 Subject: [PATCH 17/32] Remove legacy env var setting --- scripts/ds-cgpwgs.pl | 3 --- 1 file changed, 3 deletions(-) diff --git a/scripts/ds-cgpwgs.pl b/scripts/ds-cgpwgs.pl index ec7b3ce..2d78715 100644 --- a/scripts/ds-cgpwgs.pl +++ b/scripts/ds-cgpwgs.pl @@ -111,9 +111,6 @@ my $run_file = $opts{'o'}.'/run.params'; open my $FH,'>',$run_file or die "Failed to write to $run_file: $!"; -# Force explicit checking of file flush -print $FH "export PCAP_THREADED_FORCE_SYNC=1\n"; -print $FH "export PCAP_THREADED_LOADBACKOFF=1\n"; print $FH "export PCAP_THREADED_REM_LOGS=1\n"; # hard-coded printf $FH "PROTOCOL=WGS\n"; From a376cbaf0d45d9c1246cd4d63978159d6b42bc95 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 16 Apr 2019 18:32:25 +0000 Subject: [PATCH 18/32] bump allelecount for better protection against invalid index files --- build/opt-build.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 46ee6cc..17d844b 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -9,7 +9,7 @@ fi set -u ### alleleCount -VER_ALLELECOUNT="v4.0.1" +VER_ALLELECOUNT="v4.0.2" ### cgpNgsQc VER_CGPNGSQC="v1.5.1" From 4ad446e2595e5b300110c5502479f4cac00c18bf Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 16 Apr 2019 18:32:53 +0000 Subject: [PATCH 19/32] Reduce layers in final image, bump version --- Dockerfile | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/Dockerfile b/Dockerfile index 9e9d975..bcd585b 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.2 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.3 as builder USER root @@ -58,7 +58,7 @@ MAINTAINER keiranmraine@gmail.com LABEL vendor="Cancer Genome Project, Wellcome Trust Sanger Institute" LABEL uk.ac.sanger.cgp.description="CGP WGS pipeline for dockstore.org" -LABEL uk.ac.sanger.cgp.version="2.0.2" +LABEL uk.ac.sanger.cgp.version="2.1.0" RUN apt-get update -yq && \ apt-get install -qy --no-install-recommends lsb-release From c89a35c21b20dfdc990ac4d83710a7abed6e05d2 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 16 Apr 2019 18:34:01 +0000 Subject: [PATCH 20/32] changes and version bumps --- CHANGES.md | 7 +++++++ cwls/cgpwgs.cwl | 2 +- 2 files changed, 8 insertions(+), 1 deletion(-) diff --git a/CHANGES.md b/CHANGES.md index 8b05309..f8d043c 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -1,5 +1,12 @@ # CHANGES +## 2.1.0 + +* Update base image +* Upgrade allele counter and brass +* Reduce number of layers in final image. +* Remove some legacy env vars from wrappers + ## 2.0.2 * Correct skipqc flag diff --git a/cwls/cgpwgs.cwl b/cwls/cgpwgs.cwl index 869b4d7..c907065 100644 --- a/cwls/cgpwgs.cwl +++ b/cwls/cgpwgs.cwl @@ -25,7 +25,7 @@ doc: | requirements: - class: DockerRequirement - dockerPull: "quay.io/wtsicgp/dockstore-cgpwgs:2.0.2" + dockerPull: "quay.io/wtsicgp/dockstore-cgpwgs:2.1.0" hints: - class: ResourceRequirement From 5161612db36cdb78a2baf3646a824167dc57acbe Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 17 Apr 2019 14:47:00 +0000 Subject: [PATCH 21/32] Move some docs into wiki to ease user support between releases --- README.md | 48 ++++++++---------------------------------------- 1 file changed, 8 insertions(+), 40 deletions(-) diff --git a/README.md b/README.md index 7c774e8..e838fc9 100644 --- a/README.md +++ b/README.md @@ -13,10 +13,10 @@ the [Dockstore.org](https://dockstore.org/) framework. | [![Master Badge][travis-master]][travis-base] | [![Develop Badge][travis-develop]][travis-base] | * [Usage](#usage) - * [Usable Cores](#usable-cores) + * [Usable Cores](#usable-cores) * [Other uses](#other-uses) - * [Native docker](#native-docker) - * [Singularity](#singularity) + * [Native docker](#native-docker) + * [Singularity](#singularity) * [Verifying your deployment](#verifying-your-deployment) * [Example data](#example-data) * [Diagram of internals](#diagram-of-internals) @@ -76,47 +76,13 @@ If not set detects available cores on system. All of the tools installed as part of [dockstore-cgpmap][dockstore-cgpmap] and [dockstore-cgpwxs][dockstore-cgpwxs] and the above packages are available for direct use. -```bash -export CGPWGS_VER=X.X.X -docker pull quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER -docker --rm -ti [--volume ...] quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER bash -``` +See the [docker guide in the wiki][cgpmap-wiki-docker] for more details. ### Singularity -The resulting docker container has been tested with Singularity. The command to exec is: - -```bash -ds-cgpwgs.pl -h -``` - -Expected use would be along the lines of: - -```bash -export CGPWGS_VER=X.X.X -singularity pull docker://quay.io/wtsicgp/dockstore-cgpwgs:$CGPWGS_VER - -singularity exec\ - --workdir /.../workspace \ - --home /.../workspace:/home \ - --bind /.../ref/human:/var/spool/ref:ro \ - --bind /.../example_data/cgpwgs:/var/spool/data:ro \ - dockstore-cgpwgs-${CGPWGS_VER}.simg \ - ds-cgpwgs.pl \ - -reference /var/spool/ref/core_ref_GRCh37d5.tar.gz \ - -annot /var/spool/ref/VAGrENT_ref_GRCh37d5_ensembl_75.tar.gz \ - -snv_indel /var/spool/ref/SNV_INDEL_ref_GRCh37d5-fragment.tar.gz \ - -tumour /var/spool/data/COLO-829_ex.cram \ - ... -``` - -For a system automatically attaching _all local mount points_ (not default singularity behaviour) -you need not specify any `exec` params (workdir, home, bind) but you should specify the `-outdir` -option for `ds-cgpwgs.pl` to prevent data being written to your home directory. +The resulting docker container has been tested with Singularity. -By default results are written to the home directory of the container so ensure you bind -a large volume and set the `-home` variable. As indicated above the location can be overridden -via the options of `ds-cgpwgs.pl` +See the [docker guide in the wiki][cgpmap-wiki-singularity] for more details. ## Verifying your deployment @@ -200,6 +166,8 @@ identical to a statement that reads ‘Copyright (c) 2005, 2006, 2007, 2008, [caveman-wiki]: https://github.com/cancerit/cgpCaVEManWrapper/wiki [ascatngs-wiki]: https://github.com/cancerit/ascatNgs/wiki [vagrent-wiki]: https://github.com/cancerit/VAGrENT/wiki +[cgpmap-wiki-docker]: https://github.com/cancerit/dockstore-cgpwgs/wiki/Running-under-docker +[cgpmap-wiki-singularity]: https://github.com/cancerit/dockstore-cgpwgs/wiki/Running-under-singularity [travis-base]: https://travis-ci.org/cancerit/dockstore-cgpwgs From 24b602d5656a928f7bcdc4070acd4dd1447bafda Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 17 Apr 2019 14:47:34 +0000 Subject: [PATCH 22/32] pre-release test of change --- build/opt-build.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 17d844b..8b28b39 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -23,7 +23,7 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/A VER_GRASS="v2.1.1" ### BRASS -VER_BRASS="6.2.0" +VER_BRASS="hotfix/v6.2.1" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/fasta-v36.3.8g.tar.gz" From 5227db5ea5df7a055c341855f5d50a57fbc36fda Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 23 Apr 2019 12:02:17 +0000 Subject: [PATCH 23/32] Update base image to pick up hf vagrent --- CHANGES.md | 2 +- Dockerfile | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index f8d043c..2821311 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -2,7 +2,7 @@ ## 2.1.0 -* Update base image +* Update base image to dockstore-cgpwxs:3.1.4 * Upgrade allele counter and brass * Reduce number of layers in final image. * Remove some legacy env vars from wrappers diff --git a/Dockerfile b/Dockerfile index bcd585b..6a97da7 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.3 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.4 as builder USER root From 9e12c9130433e643013cb9900541a1c539142b1d Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 24 Apr 2019 10:49:46 +0000 Subject: [PATCH 24/32] Test of this looks good, move to tagged --- build/opt-build.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 8b28b39..5feffe7 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -23,7 +23,7 @@ SRC_ASCAT="https://raw.githubusercontent.com/Crick-CancerGenomics/ascat/v2.5.1/A VER_GRASS="v2.1.1" ### BRASS -VER_BRASS="hotfix/v6.2.1" +VER_BRASS="v6.2.1" SOURCE_BLAT="http://users.soe.ucsc.edu/~kent/src/blatSrc35.zip" SRC_FASTA36="https://github.com/wrpearson/fasta36/archive/fasta-v36.3.8g.tar.gz" From 778b23a40eebd34ecf78034045e10adbdc96b737 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 24 Apr 2019 10:55:53 +0000 Subject: [PATCH 25/32] More detail in changelog --- CHANGES.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CHANGES.md b/CHANGES.md index 2821311..9c7d06c 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -6,6 +6,10 @@ * Upgrade allele counter and brass * Reduce number of layers in final image. * Remove some legacy env vars from wrappers +* Battenberg removed + * Was only included to do base allele counts not complete analysis + * Underlying data files are not supported for new builds or other species + * Contact [original authors](https://github.com/Wedge-Oxford/battenberg) for ongoing support ## 2.0.2 From fe11284f6ce4a1922d933c8775af376525c0940e Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 26 Apr 2019 08:06:37 +0000 Subject: [PATCH 26/32] Pass in path to gender loci --- scripts/analysisWGS.sh | 1 + 1 file changed, 1 insertion(+) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index c91a4f1..9370c40 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -233,6 +233,7 @@ do_parallel[ascat]="ascat.pl \ -r $REF_BASE/genome.fa \ -q 20 \ -g L \ + -l $REF_BASE/gender.tsv \ -rs '$SPECIES' \ -ra $ASSEMBLY \ -pr $PROTOCOL \ From 02762f221c2895d581e35ceba1e529304f89de5d Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 29 Apr 2019 13:23:59 +0000 Subject: [PATCH 27/32] Fix failure to use normal contamination value from ASCAT and make flagging run in parallel --- CHANGES.md | 5 +++++ Dockerfile | 2 +- scripts/analysisWGS.sh | 36 ++++++++++++++++++++++++------------ 3 files changed, 30 insertions(+), 13 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index 9c7d06c..db90e9c 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -10,6 +10,11 @@ * Was only included to do base allele counts not complete analysis * Underlying data files are not supported for new builds or other species * Contact [original authors](https://github.com/Wedge-Oxford/battenberg) for ongoing support +* Wrapper script didn't explicitly specify sex SNP loci file (ASCAT) + * no impact for GRCh37 other species/build would have used wrong sex chromosome SNPs +* CaVEMan step wasn't being passed normal contamination value, scientific impact: + * previously `caveman.pl` default of `0.1` was applied + * this will now honor the value from ASCAT (`1-$rho`) ## 2.0.2 diff --git a/Dockerfile b/Dockerfile index 6a97da7..ee0ce23 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.4 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.5 as builder USER root diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index 9370c40..c925dce 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -284,6 +284,7 @@ set -x ASCAT_CN="$OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/$NAME_MT.copynumber.caveman.csv" perl -ne '@F=(split q{,}, $_)[1,2,3,4]; $F[1]-1; print join("\t",@F)."\n";' < $ASCAT_CN > $TMP/norm.cn.bed perl -ne '@F=(split q{,}, $_)[1,2,3,6]; $F[1]-1; print join("\t",@F)."\n";' < $ASCAT_CN > $TMP/tum.cn.bed +NORM_CONTAM=`perl -ne 'if(m/^rho\s(.+)\n/){print 1-$1;}' $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/$NAME_MT` set +x # unset and redeclare the parallel array ready for next block @@ -330,7 +331,8 @@ do_parallel[CaVEMan]="caveman.pl \ -e $CAVESPLIT \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman \ -x $CONTIG_EXCLUDE \ - -no-flagging" + -k $NORM_CONTAM \ + -no-flagging -noclean" echo "Starting Parallel block 4: `date`" run_parallel do_parallel @@ -373,20 +375,30 @@ do_parallel[cgpPindel_annot]="AnnotateVcf.pl -t -c $REF_BASE/vagrent/vagrent.cac -i $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/pindel/${NAME_MT}_vs_${NAME_WT}.flagged.vcf.gz \ -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/pindel/${NAME_MT}_vs_${NAME_WT}.annot.vcf" -echo -e "\t[Parallel block 5] cgpFlagCaVEMan added..." -do_parallel[cgpFlagCaVEMan]="cgpFlagCaVEMan.pl \ - -i $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman/${NAME_MT}_vs_${NAME_WT}.muts.ids.vcf.gz \ - -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman/${NAME_MT}_vs_${NAME_WT}.flagged.muts.vcf \ - -s '$SPECIES' \ - -m $BAM_MT_TMP \ - -n $BAM_WT_TMP \ +echo -e "\t[Parallel block 5] CaVEMan flag added..." +do_parallel[CaVEMan]="caveman.pl \ + -r $REF_BASE/genome.fa.fai \ + -ig $REF_BASE/caveman/HiDepth.tsv \ -b $REF_BASE/caveman/flagging \ - -g $GERMLINE_BED.gz \ - -umv $REF_BASE/caveman \ -ab $REF_BASE/vagrent \ - -ref $REF_BASE/genome.fa.fai \ + -u $REF_BASE/caveman \ + -s '$SPECIES' \ + -sa $ASSEMBLY \ + -t $CPU \ + -st $PROTOCOL \ + -tc $TMP/tum.cn.bed \ + -nc $TMP/norm.cn.bed \ + -td 5 -nd 2 \ + -tb $BAM_MT_TMP \ + -nb $BAM_WT_TMP \ -c $SNVFLAG \ - -v $REF_BASE/caveman/flagging/flag.to.vcf.convert.ini" + -f $REF_BASE/caveman/flagging/flag.to.vcf.convert.ini \ + -e $CAVESPLIT \ + -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman \ + -x $CONTIG_EXCLUDE \ + -k $NORM_CONTAM \ + -in $GERMLINE_BED.gz \ + -p flag" echo "Starting Parallel block 5: `date`" run_parallel do_parallel From 072e072546a76fc39b81fd167601b80494b4e036 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Mon, 29 Apr 2019 17:07:56 +0000 Subject: [PATCH 28/32] Correct path to file --- scripts/analysisWGS.sh | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index c925dce..f0e56f2 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -284,7 +284,7 @@ set -x ASCAT_CN="$OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/$NAME_MT.copynumber.caveman.csv" perl -ne '@F=(split q{,}, $_)[1,2,3,4]; $F[1]-1; print join("\t",@F)."\n";' < $ASCAT_CN > $TMP/norm.cn.bed perl -ne '@F=(split q{,}, $_)[1,2,3,6]; $F[1]-1; print join("\t",@F)."\n";' < $ASCAT_CN > $TMP/tum.cn.bed -NORM_CONTAM=`perl -ne 'if(m/^rho\s(.+)\n/){print 1-$1;}' $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/$NAME_MT` +NORM_CONTAM=`perl -ne 'if(m/^rho\s(.+)\n/){print 1-$1;}' $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/ascat/$NAME_MT.samplestatistics.txt` set +x # unset and redeclare the parallel array ready for next block From 4b9d5ba4813e35ea814bc3e2f389e3b55fb250a5 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Wed, 1 May 2019 07:51:21 +0000 Subject: [PATCH 29/32] Already compressed and indexed --- scripts/analysisWGS.sh | 6 +----- 1 file changed, 1 insertion(+), 5 deletions(-) diff --git a/scripts/analysisWGS.sh b/scripts/analysisWGS.sh index f0e56f2..14cfc3e 100755 --- a/scripts/analysisWGS.sh +++ b/scripts/analysisWGS.sh @@ -376,7 +376,7 @@ do_parallel[cgpPindel_annot]="AnnotateVcf.pl -t -c $REF_BASE/vagrent/vagrent.cac -o $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/pindel/${NAME_MT}_vs_${NAME_WT}.annot.vcf" echo -e "\t[Parallel block 5] CaVEMan flag added..." -do_parallel[CaVEMan]="caveman.pl \ +do_parallel[CaVEMan_flag]="caveman.pl \ -r $REF_BASE/genome.fa.fai \ -ig $REF_BASE/caveman/HiDepth.tsv \ -b $REF_BASE/caveman/flagging \ @@ -403,10 +403,6 @@ do_parallel[CaVEMan]="caveman.pl \ echo "Starting Parallel block 5: `date`" run_parallel do_parallel -# compress and index flagged caveman -bgzip $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman/${NAME_MT}_vs_${NAME_WT}.flagged.muts.vcf -tabix -p vcf $OUTPUT_DIR/${PROTOCOL}_${NAME_MT}_vs_${NAME_WT}/caveman/${NAME_MT}_vs_${NAME_WT}.flagged.muts.vcf.gz - # unset and redeclare the parallel array ready for next block unset do_parallel declare -A do_parallel From 097c84d7e67930f5e495da8d8b225101d7f0a198 Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Thu, 2 May 2019 15:34:26 +0000 Subject: [PATCH 30/32] Lowest resistance test of pindel speedup --- build/opt-build.sh | 23 +++++++++++++++++++++++ 1 file changed, 23 insertions(+) diff --git a/build/opt-build.sh b/build/opt-build.sh index 5feffe7..01fcbe0 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -217,6 +217,29 @@ if [ ! -e $SETUP_DIR/BRASS.success ]; then touch $SETUP_DIR/BRASS.success fi + +### HACK_REMOVE ME - cgpPindel +VER_CGPPINDEL=feature/speedUpGRCh38 +if [ ! -e $SETUP_DIR/cgpPindel.success ]; then + curl -sSL --retry 10 https://github.com/cancerit/cgpPindel/archive/${VER_CGPPINDEL}.tar.gz > distro.tar.gz + rm -rf distro/* + tar --strip-components 1 -C distro -xzf distro.tar.gz + cd distro + if [ ! -e $SETUP_DIR/cgpPindel_c.success ]; then + g++ -O3 -o $INST_PATH/bin/pindel c++/pindel.cpp + g++ -O3 -o $INST_PATH/bin/filter_pindel_reads c++/filter_pindel_reads.cpp + touch $SETUP_DIR/cgpPindel_c.success + fi + cd perl + cpanm --no-interactive --notest --mirror http://cpan.metacpan.org --notest -l $INST_PATH --installdeps . + cpanm -v --no-interactive --mirror http://cpan.metacpan.org -l $INST_PATH . + cd $SETUP_DIR + rm -rf distro.* distro/* + touch $SETUP_DIR/cgpPindel.success +fi + + + cd $HOME rm -rf $SETUP_DIR From 5d9e196d8926c7199d41aab67376e257adf31def Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Fri, 3 May 2019 08:04:27 +0000 Subject: [PATCH 31/32] Revert pindel test --- build/opt-build.sh | 23 ----------------------- 1 file changed, 23 deletions(-) diff --git a/build/opt-build.sh b/build/opt-build.sh index 01fcbe0..5feffe7 100644 --- a/build/opt-build.sh +++ b/build/opt-build.sh @@ -217,29 +217,6 @@ if [ ! -e $SETUP_DIR/BRASS.success ]; then touch $SETUP_DIR/BRASS.success fi - -### HACK_REMOVE ME - cgpPindel -VER_CGPPINDEL=feature/speedUpGRCh38 -if [ ! -e $SETUP_DIR/cgpPindel.success ]; then - curl -sSL --retry 10 https://github.com/cancerit/cgpPindel/archive/${VER_CGPPINDEL}.tar.gz > distro.tar.gz - rm -rf distro/* - tar --strip-components 1 -C distro -xzf distro.tar.gz - cd distro - if [ ! -e $SETUP_DIR/cgpPindel_c.success ]; then - g++ -O3 -o $INST_PATH/bin/pindel c++/pindel.cpp - g++ -O3 -o $INST_PATH/bin/filter_pindel_reads c++/filter_pindel_reads.cpp - touch $SETUP_DIR/cgpPindel_c.success - fi - cd perl - cpanm --no-interactive --notest --mirror http://cpan.metacpan.org --notest -l $INST_PATH --installdeps . - cpanm -v --no-interactive --mirror http://cpan.metacpan.org -l $INST_PATH . - cd $SETUP_DIR - rm -rf distro.* distro/* - touch $SETUP_DIR/cgpPindel.success -fi - - - cd $HOME rm -rf $SETUP_DIR From 11c538120554e6b35775599189a3c0f7a8c8ec8a Mon Sep 17 00:00:00 2001 From: Keiran Raine Date: Tue, 7 May 2019 11:52:32 +0000 Subject: [PATCH 32/32] Bump base image and detail changes --- CHANGES.md | 6 +++++- Dockerfile | 2 +- 2 files changed, 6 insertions(+), 2 deletions(-) diff --git a/CHANGES.md b/CHANGES.md index db90e9c..3ae3015 100644 --- a/CHANGES.md +++ b/CHANGES.md @@ -2,7 +2,11 @@ ## 2.1.0 -* Update base image to dockstore-cgpwxs:3.1.4 +* Update base image to dockstore-cgpwxs:3.1.6 (from 3.0.3) + * Includes, memory footprint reduction for Caveman flagging + * Significant speedup in inde input generation + * Parallel flagging for SNV + * Fragment based SNV/Indel calling * Upgrade allele counter and brass * Reduce number of layers in final image. * Remove some legacy env vars from wrappers diff --git a/Dockerfile b/Dockerfile index ee0ce23..841d807 100644 --- a/Dockerfile +++ b/Dockerfile @@ -1,4 +1,4 @@ -FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.5 as builder +FROM quay.io/wtsicgp/dockstore-cgpwxs:3.1.6 as builder USER root

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