Question about SILAC data analysis

[giuliof95]

Hi, I wanted to try your tool for my SILAC data and I'm currently setting the keys.txt file, I hust have a quick question: My heavy and light were mixed 1:1 before my LC-MS run and were analysed in MaxQuant. What should I put in the isotopelabeltype column?

I also would like to know how should i name my samples if I also made fractionation of my mixture. I have 3 biological replicates, 9 fractions and 2 tecnical replicates each. It is kind of "weird" and I don't know how to set the txt file.

Thanks for the kind attention,

Giulio