From 4afcc879b542312f292cee716aa2049a0d03c2f8 Mon Sep 17 00:00:00 2001 From: chengxin Dai <37200167+daichengxin@users.noreply.github.com> Date: Mon, 11 Sep 2023 13:38:56 +0800 Subject: [PATCH 1/4] fixed bug --- pmultiqc/modules/quantms/quantms.py | 5 ++--- 1 file changed, 2 insertions(+), 3 deletions(-) diff --git a/pmultiqc/modules/quantms/quantms.py b/pmultiqc/modules/quantms/quantms.py index 0c336de..6dd30b9 100755 --- a/pmultiqc/modules/quantms/quantms.py +++ b/pmultiqc/modules/quantms/quantms.py @@ -145,8 +145,7 @@ def __init__(self): self.mzml_info_path.sort() if len(self.mzml_info_path) > 0: self.read_mzml_info = True - self.mzML_paths = [os.path.basename(i.rstrip("_mzml_info.tsv") + ".mzML") for i in self.mzml_info_path] - + self.mzML_paths = [os.path.basename(i).replace("_mzml_info.tsv", ".mzML") for i in self.mzml_info_path] for f in self.find_log_files("quantms/mztab", filecontents=False): self.out_mzTab_path = os.path.join(f["root"], f['fn']) self.parse_out_mzTab() @@ -1092,7 +1091,7 @@ def read_mzml_info(): log.info("{}: Parsing mzml_statistics dataframe {}...".format(datetime.now().strftime("%H:%M:%S"), file)) mzml_df = pd.read_csv(file, sep="\t") - m = os.path.basename(file.rstrip("_mzml_info.tsv") + ".mzML") + m = os.path.basename(file).replace("_mzml_info.tsv", ".mzML") if m not in mzml_table: mzml_table[m] = dict.fromkeys(['MS1_Num', 'MS2_Num', 'Charge_2'], 0) charge_group = mzml_df.groupby("Charge").size() From f3d67b685d79a324536e7868e2affa5e3898a31e Mon Sep 17 00:00:00 2001 From: Hong Wang <88552471+WangHong007@users.noreply.github.com> Date: Wed, 13 Sep 2023 08:57:53 +0800 Subject: [PATCH 2/4] Use the file prefix as the pattern --- pmultiqc/modules/quantms/quantms.py | 161 +++++++++++++++------------- 1 file changed, 86 insertions(+), 75 deletions(-) diff --git a/pmultiqc/modules/quantms/quantms.py b/pmultiqc/modules/quantms/quantms.py index 0c336de..287815e 100755 --- a/pmultiqc/modules/quantms/quantms.py +++ b/pmultiqc/modules/quantms/quantms.py @@ -57,6 +57,14 @@ class QuantMSModule(BaseMultiqcModule): + @staticmethod + def file_prefix(path): + try: + return os.path.splitext(os.path.basename(path))[0] + except: + raise SystemExit("Illegal file path: {path}") + + def __init__(self): # Initialise the parent module Class object @@ -96,7 +104,7 @@ def __init__(self): self.PSM_table = dict() self.mzml_peptide_map = dict() self.identified_spectrum = dict() - self.mL_with_psm = list() + self.ms_with_psm = list() self.pep_quant_table = dict() self.read_mzml_info = False self.mzml_table = OrderedDict() @@ -135,9 +143,9 @@ def __init__(self): self.pep_table_exists = False self.enable_dia = False - self.mzML_paths = [] + self.ms_paths = [] for mzML_file in self.find_log_files("quantms/mzML", filecontents=False): - self.mzML_paths.append(os.path.join(mzML_file["root"], mzML_file['fn'])) + self.ms_paths.append(os.path.join(mzML_file["root"], mzML_file['fn'])) self.mzml_info_path = [] for mzml_info in self.find_log_files("quantms/mzml_info", filecontents=False): @@ -145,7 +153,7 @@ def __init__(self): self.mzml_info_path.sort() if len(self.mzml_info_path) > 0: self.read_mzml_info = True - self.mzML_paths = [os.path.basename(i.rstrip("_mzml_info.tsv") + ".mzML") for i in self.mzml_info_path] + self.ms_paths = [self.file_prefix(i).replace("_mzml_info", ".mzML") for i in self.mzml_info_path] for f in self.find_log_files("quantms/mztab", filecontents=False): self.out_mzTab_path = os.path.join(f["root"], f['fn']) @@ -165,9 +173,10 @@ def __init__(self): self.draw_summary_protein_ident_table() self.draw_quantms_identi_num() self.draw_num_pep_per_protein() - self.draw_precursor_charge_distribution() - self.draw_peaks_per_ms2() - self.draw_peak_intensity_distribution() + if len(self.mzml_info_path) > 0: + self.draw_precursor_charge_distribution() + self.draw_peaks_per_ms2() + self.draw_peak_intensity_distribution() else: self.parse_idxml(mt) self.CalHeatMapScore() @@ -242,6 +251,7 @@ def __init__(self): os.path.join(os.path.dirname(__file__), 'assets', 'js', 'sql-optimized.js') } + def draw_heatmap(self): HeatMapScore = [] if self.pep_table_exists: @@ -249,7 +259,7 @@ def draw_heatmap(self): 'ID rate over RT', 'MS2 OverSampling', 'Pep Missing Values'] ynames = [] for k, v in self.heatmap_con_score.items(): - if k in self.mL_with_psm: + if k in self.ms_with_psm: ynames.append(k) HeatMapScore.append([v, self.heatmap_pep_intensity[k], self.heatmap_charge_score[k], self.MissedCleavages_heatmap_score[k], @@ -260,7 +270,7 @@ def draw_heatmap(self): 'Pep Missing Values'] ynames = [] for k, v in self.heatmap_charge_score.items(): - if k in self.mL_with_psm: + if k in self.ms_with_psm: ynames.append(k) HeatMapScore.append([self.heatmap_charge_score[k], self.MissedCleavages_heatmap_score[k], self.MissedCleavagesVar_score[k], self.ID_RT_score[k], @@ -315,18 +325,17 @@ def draw_exp_design(self): # One table format would actually be even easier. You can just use pandas.read_tsv self.sample_df, self.file_df = read_openms_design(self.exp_design) - for file in np.unique(self.file_df["Spectra_Filepath"].tolist()): - stand_file = os.path.basename(file) - file_index = self.file_df[self.file_df["Spectra_Filepath"] == file] - self.exp_design_table[stand_file] = {'Fraction_Group': file_index["Fraction_Group"].tolist()[0]} - self.exp_design_table[stand_file]['Fraction'] = file_index["Fraction"].tolist()[0] - self.exp_design_table[stand_file]['Label'] = '|'.join(file_index["Label"]) + for file in np.unique(self.file_df["Run"].tolist()): + file_index = self.file_df[self.file_df["Run"] == file] + self.exp_design_table[file] = {'Fraction_Group': file_index["Fraction_Group"].tolist()[0]} + self.exp_design_table[file]['Fraction'] = file_index["Fraction"].tolist()[0] + self.exp_design_table[file]['Label'] = '|'.join(file_index["Label"]) sample = file_index["Sample"].tolist() - self.exp_design_table[stand_file]['Sample'] = '|'.join(sample) - self.exp_design_table[stand_file]['MSstats_Condition'] = ','.join( + self.exp_design_table[file]['Sample'] = '|'.join(sample) + self.exp_design_table[file]['MSstats_Condition'] = ','.join( [row['MSstats_Condition'] for _, row in self.sample_df.iterrows() if row['Sample'] in sample]) - self.exp_design_table[stand_file]['MSstats_BioReplicate'] = '|'.join( + self.exp_design_table[file]['MSstats_BioReplicate'] = '|'.join( [row['MSstats_BioReplicate'] for _, row in self.sample_df.iterrows() if row['Sample'] in sample]) @@ -896,7 +905,7 @@ def CalHeatMapScore(self): if self.pep_table_exists: pep_table = mztab_data.peptide_table pep_table.loc[:, 'stand_spectra_ref'] = pep_table.apply( - lambda x: os.path.basename(meta_data[x.spectra_ref.split(':')[0] + '-location']), axis=1) + lambda x: self.file_prefix(meta_data[x.spectra_ref.split(':')[0] + '-location']), axis=1) study_variables = list(filter(lambda x: re.match(r'peptide_abundance_study_variable.*?', x) is not None, pep_table.columns.tolist())) @@ -918,7 +927,7 @@ def CalHeatMapScore(self): if config.kwargs['remove_decoy'] and 'opt_global_cv_MS:1002217_decoy_peptide' in psm.columns: psm = psm[psm['opt_global_cv_MS:1002217_decoy_peptide'] == 0] psm.loc[:, 'stand_spectra_ref'] = psm.apply( - lambda x: os.path.basename(meta_data[x.spectra_ref.split(':')[0] + '-location']), axis=1) + lambda x: self.file_prefix(meta_data[x.spectra_ref.split(':')[0] + '-location']), axis=1) enzyme_list = [i for i in meta_data.values() if str(i).startswith("enzyme:")] enzyme = enzyme_list[0].split(":")[1] if len(enzyme_list) == 1 else "Trypsin" @@ -1009,9 +1018,9 @@ def parse_mzml(self): mzml_table = {} heatmap_charge = {} - self.mL_without_psm = [] + self.ms_without_psm = [] - def add_mzml_values(info_df, mzml_name): + def add_ms_values(info_df, ms_name): charge_state = int(info_df["Charge"]) if info_df["Charge"] != None else None base_peak_intensity = float(info_df['Base_Peak_Intensity']) if info_df['Base_Peak_Intensity'] != None else None peak_per_ms2 = int(info_df['MS2_peaks']) if info_df['MS2_peaks'] != None else None @@ -1022,8 +1031,8 @@ def add_mzml_values(info_df, mzml_name): self.mzml_peaks_ms2_plot.addValue(peak_per_ms2) return - if mzml_name in self.mL_with_psm: - if info_df["SpectrumID"] in self.identified_spectrum[mzml_name]: + if ms_name in self.ms_with_psm: + if info_df["SpectrumID"] in self.identified_spectrum[ms_name]: self.mzml_charge_plot.addValue(charge_state) self.mzml_peak_distribution_plot.addValue(base_peak_intensity) self.mzml_peaks_ms2_plot.addValue(peak_per_ms2) @@ -1032,18 +1041,18 @@ def add_mzml_values(info_df, mzml_name): self.mzml_peak_distribution_plot_1.addValue(base_peak_intensity) self.mzml_peaks_ms2_plot_1.addValue(peak_per_ms2) else: - if mzml_name not in self.mL_without_psm: - self.mL_without_psm.append(mzml_name) + if ms_name not in self.ms_without_psm: + self.ms_without_psm.append(ms_name) def read_mzmls(): exp = MSExperiment() - for m in self.mzML_paths: + for m in self.ms_paths: ms1_number = 0 ms2_number = 0 log.info("{}: Parsing mzML file {}...".format(datetime.now().strftime("%H:%M:%S"), m)) MzMLFile().load(m, exp) log.info("{}: Done parsing mzML file {}...".format(datetime.now().strftime("%H:%M:%S"), m)) - m = os.path.basename(m) + m = self.file_prefix(m) log.info("{}: Aggregating mzML file {}...".format(datetime.now().strftime("%H:%M:%S"), m)) charge_2 = 0 for i in exp: @@ -1068,7 +1077,7 @@ def read_mzmls(): self.mzml_peaks_ms2_plot.addValue(peak_per_ms2) continue - if m in self.mL_with_psm: + if m in self.ms_with_psm: if i.getNativeID() in self.identified_spectrum[m]: self.mzml_charge_plot.addValue(charge_state) self.mzml_peak_distribution_plot.addValue(base_peak_intensity) @@ -1078,8 +1087,8 @@ def read_mzmls(): self.mzml_peak_distribution_plot_1.addValue(base_peak_intensity) self.mzml_peaks_ms2_plot_1.addValue(peak_per_ms2) else: - if m not in self.mL_without_psm: - self.mL_without_psm.append(m) + if m not in self.ms_without_psm: + self.ms_without_psm.append(m) heatmap_charge[m] = charge_2 / ms2_number self.total_ms2_spectra = self.total_ms2_spectra + ms2_number @@ -1092,7 +1101,7 @@ def read_mzml_info(): log.info("{}: Parsing mzml_statistics dataframe {}...".format(datetime.now().strftime("%H:%M:%S"), file)) mzml_df = pd.read_csv(file, sep="\t") - m = os.path.basename(file.rstrip("_mzml_info.tsv") + ".mzML") + m = self.file_prefix(file).replace("_mzml_info", "") if m not in mzml_table: mzml_table[m] = dict.fromkeys(['MS1_Num', 'MS2_Num', 'Charge_2'], 0) charge_group = mzml_df.groupby("Charge").size() @@ -1107,14 +1116,14 @@ def read_mzml_info(): group = mzml_df[mzml_df["MSLevel"] == 2] del mzml_df - group.apply(add_mzml_values, args=(m,), axis=1) + group.apply(add_ms_values, args=(m,), axis=1) for m in mzml_table.keys(): heatmap_charge[m] = mzml_table[m]['Charge_2'] / mzml_table[m]['MS2_Num'] log.info("{}: Done aggregating mzml_statistics dataframe {}...".format(datetime.now().strftime("%H:%M:%S"), file)) read_mzml_info() if self.read_mzml_info else read_mzmls() - for i in self.mL_without_psm: + for i in self.ms_without_psm: log.warning("No PSM found in '{}'!".format(i)) self.mzml_peaks_ms2_plot.to_dict() @@ -1181,7 +1190,7 @@ def parse_idxml(self, mzml_table): protein_ids = [] peptide_ids = [] IdXMLFile().load(raw_id, protein_ids, peptide_ids) - raw_id = os.path.basename(raw_id) + raw_id = self.file_prefix(raw_id) ## TODO I would use the QC functionality of pyopenms. Should be much faster. if config.kwargs['remove_decoy']: identified_num = len(set([i.getMetaValue("spectrum_reference") for i in peptide_ids @@ -1190,7 +1199,7 @@ def parse_idxml(self, mzml_table): identified_num = len(peptide_ids) ## TODO make clear if this is before or after first step of filtering - mzML_name = os.path.basename(protein_ids[0].getMetaValue("spectra_data")[0].decode("UTF-8")) + ms_name = self.file_prefix(protein_ids[0].getMetaValue("spectra_data")[0].decode("UTF-8")) search_engine = protein_ids[0].getSearchEngine() self.search_engine['SpecE'][raw_id] = OrderedDict() @@ -1215,7 +1224,7 @@ def parse_idxml(self, mzml_table): Consensus_support = Histogram('Consensus PSM number', plot_category = 'frequency', stacks = bar_stacks) if search_engine == "MSGF+" or "msgf" in raw_id: - mzml_table[mzML_name]['MSGF'] = identified_num + mzml_table[ms_name]['MSGF'] = identified_num self.MSGF_label = True SpecE_label.append({'name': raw_id, 'ylab': 'Counts'}) PEPs_label.append({'name': raw_id, 'ylab': 'Counts'}) @@ -1234,7 +1243,7 @@ def parse_idxml(self, mzml_table): elif search_engine == "Comet" or "comet" in raw_id: self.Comet_label = True - mzml_table[mzML_name]['Comet'] = identified_num + mzml_table[ms_name]['Comet'] = identified_num xcorr_label.append({'name': raw_id, 'ylab': 'Counts'}) PEPs_label.append({'name': raw_id, 'ylab': 'Counts'}) for peptide_id in peptide_ids: @@ -1250,17 +1259,17 @@ def parse_idxml(self, mzml_table): self.search_engine['PEPs'][raw_id] = PEP.dict['data'] else: - mzml_table[mzML_name][search_engine] = identified_num + mzml_table[ms_name][search_engine] = identified_num - mzml_table[mzML_name]['num_quant_psms'] = self.mL_spec_ident_final[mzML_name] if mzML_name in self.mL_spec_ident_final.keys() else 0 - mzml_table[mzML_name]['num_quant_peps'] = len(self.mzml_peptide_map[mzML_name]) if mzML_name in self.mL_spec_ident_final.keys() else 0 + mzml_table[ms_name]['num_quant_psms'] = self.mL_spec_ident_final[ms_name] if ms_name in self.mL_spec_ident_final.keys() else 0 + mzml_table[ms_name]['num_quant_peps'] = len(self.mzml_peptide_map[ms_name]) if ms_name in self.mL_spec_ident_final.keys() else 0 for raw_id in consensus_paths: log.info("Parsing consensus file {}...".format(raw_id)) protein_ids = [] peptide_ids = [] IdXMLFile().load(raw_id, protein_ids, peptide_ids) - raw_id = os.path.basename(raw_id) + raw_id = self.file_prefix(raw_id) consensus_label.append({'name': raw_id, 'ylab': 'Counts'}) self.search_engine['consensus_support'][raw_id] = OrderedDict() @@ -1275,9 +1284,9 @@ def parse_idxml(self, mzml_table): self.search_engine['data_label'] = {'score_label': [SpecE_label, xcorr_label], 'PEPs_label': PEPs_label, 'consensus_label': consensus_label} - # mzml file sorted based on experimental file - for mzML_name in self.exp_design_table.keys(): - self.mzml_table[mzML_name] = mzml_table[mzML_name] + # mass spectrum files sorted based on experimental file + for spectrum_name in self.exp_design_table.keys(): + self.mzml_table[spectrum_name] = mzml_table[spectrum_name] def parse_out_mzTab(self): log.info("{}: Parsing mzTab file {}...".format(datetime.now().strftime("%H:%M:%S"), self.out_mzTab_path)) @@ -1303,8 +1312,8 @@ def parse_out_mzTab(self): psm['stand_spectra_ref'] = psm.apply( lambda x: os.path.basename(meta_data[x.spectra_ref.split(':')[0] + '-location']) + ":" + x.spectra_ref.split(':')[1], axis=1) psm['filename'] = psm.apply( - lambda x: os.path.basename(meta_data[x.spectra_ref.split(':')[0] + '-location']), axis=1) - self.mL_with_psm = psm['filename'].unique().tolist() + lambda x: self.file_prefix(meta_data[x.spectra_ref.split(':')[0] + '-location']), axis=1) + self.ms_with_psm = psm['filename'].unique().tolist() prot = mztab_data.protein_table self.prot_search_score = dict() @@ -1364,6 +1373,7 @@ def parse_out_mzTab(self): mL_spec_ident_final = {} for m, group in psm.groupby('filename'): + m = os.path.basename(m) self.cal_num_table_data[m] = {'sample_name': self.exp_design_table[m]['Sample']} self.cal_num_table_data[m]['condition'] = self.exp_design_table[m]['MSstats_Condition'] self.cal_num_table_data[m]['fraction'] = self.exp_design_table[m]['Fraction'] @@ -1402,8 +1412,8 @@ def parse_out_mzTab(self): mL_spec_ident_final[m] = len(set(self.identified_spectrum[m])) # TODO mzMLs without PSM: experimental design information is displayed, and all quantitative information is 0 - self.mL_without_psm = set([os.path.basename(i) for i in self.mzML_paths]) - set(self.mL_with_psm) - for i in self.mL_without_psm: + self.ms_without_psm = set([self.file_prefix(i) for i in self.ms_paths]) - set(self.ms_with_psm) + for i in self.ms_without_psm: self.cal_num_table_data[i] = {'sample_name': self.exp_design_table[i]['Sample'], 'condition': self.exp_design_table[i]['MSstats_Condition'], 'fraction': self.exp_design_table[i]['Fraction'], @@ -1683,8 +1693,8 @@ def parse_diann_report(self): self.pep_plot.to_dict(percentage = True, cats = categorys) for run_file, group in report_data.groupby("File.Name"): - run_file = os.path.basename(run_file) - self.mL_with_psm.append(run_file) + run_file = self.file_prefix(run_file) + self.ms_with_psm.append(run_file) self.cal_num_table_data[run_file] = {'sample_name': self.exp_design_table[run_file]['Sample']} self.cal_num_table_data[run_file]['condition'] = self.exp_design_table[run_file]['MSstats_Condition'] self.cal_num_table_data[run_file]['fraction'] = self.exp_design_table[run_file]['Fraction'] @@ -1697,11 +1707,11 @@ def parse_diann_report(self): self.cal_num_table_data[run_file]['unique_peptide_num'] = len(unique_peptides) self.cal_num_table_data[run_file]['modified_peptide_num'] = len(modified_pep) - self.mL_without_psm = set([os.path.basename(i) for i in self.mzML_paths]) - set(self.mL_with_psm) - for i in self.mL_without_psm: + self.ms_without_psm = set([self.file_prefix(i) for i in self.ms_paths]) - set(self.ms_with_psm) + for i in self.ms_without_psm: log.warning("No PSM found in '{}'!".format(i)) - for i in self.mL_without_psm: + for i in self.ms_without_psm: self.cal_num_table_data[i] = {'sample_name': self.exp_design_table[i]['Sample'], 'condition': self.exp_design_table[i]['MSstats_Condition'], 'fraction': self.exp_design_table[i]['Fraction'], @@ -1985,29 +1995,29 @@ def uniqueCount(series): ) def read_openms_design(desfile): - with open(desfile, 'r') as f: - data = f.readlines() - s_row = False - f_table = [] - s_table = [] - for row in data: - if row == "\n": - continue - if "MSstats_Condition" in row: - s_row = True - s_header = row.replace('\n', '').split('\t') - elif s_row: - s_table.append(row.replace('\n', '').split('\t')) - elif "Spectra_Filepath" in row: - f_header = row.replace('\n', '').split('\t') - else: - f_table.append(row.replace('\n', '').split('\t')) + with open(desfile, 'r') as f: + data = f.readlines() + s_row = False + f_table = [] + s_table = [] + for row in data: + if row == "\n": + continue + if "MSstats_Condition" in row: + s_row = True + s_header = row.replace('\n', '').split('\t') + elif s_row: + s_table.append(row.replace('\n', '').split('\t')) + elif "Spectra_Filepath" in row: + f_header = row.replace('\n', '').split('\t') + else: + f_table.append(row.replace('\n', '').split('\t')) - f_table = pd.DataFrame(f_table, columns=f_header) - f_table["Run"] = f_table.apply(lambda x: os.path.splitext(os.path.basename(x["Spectra_Filepath"]))[0], axis=1) - s_DataFrame = pd.DataFrame(s_table, columns=s_header) + f_table = pd.DataFrame(f_table, columns=f_header) + f_table["Run"] = f_table.apply(lambda x: QuantMSModule.file_prefix(x["Spectra_Filepath"]), axis=1) + s_DataFrame = pd.DataFrame(s_table, columns=s_header) - return s_DataFrame, f_table + return s_DataFrame, f_table def find_modification(peptide): peptide = str(peptide) @@ -2024,3 +2034,4 @@ def find_modification(peptide): original_mods = ",".join(str(i) for i in original_mods) if len(original_mods) > 0 else "nan" return AASequence.fromString(peptide).toUnmodifiedString(), original_mods + From 54966d3a558b3ee3610fdf4180099e002a4e9ee8 Mon Sep 17 00:00:00 2001 From: chengxin Dai <37200167+daichengxin@users.noreply.github.com> Date: Fri, 15 Sep 2023 10:44:50 +0800 Subject: [PATCH 3/4] fixed json type error --- ...HeLacells_consensus_idXML_ylab_Counts_.pdf | Bin 0 -> 11757 bytes ...HeLacells_consensus_idXML_ylab_Counts_.pdf | Bin 0 -> 11757 bytes ...ame_Blank_consensus_idXML_ylab_Counts_.pdf | Bin 0 -> 11757 bytes ...nglecell1_consensus_idXML_ylab_Counts_.pdf | Bin 0 -> 11757 bytes ...nglecell2_consensus_idXML_ylab_Counts_.pdf | Bin 0 -> 11757 bytes ...nglecell3_consensus_idXML_ylab_Counts_.pdf | Bin 0 -> 11757 bytes ...nglecell4_consensus_idXML_ylab_Counts_.pdf | Bin 0 -> 11757 bytes ...lls_comet_feat_perc_idXML_ylab_Counts_.pdf | Bin 0 -> 17660 bytes ...ells_msgf_feat_perc_idXML_ylab_Counts_.pdf | Bin 0 -> 17613 bytes ...lls_comet_feat_perc_idXML_ylab_Counts_.pdf | Bin 0 -> 17598 bytes 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- - - - - - - - 2023-09-15T08:24:34.804772 - image/svg+xml - - - Matplotlib v3.3.4, https://matplotlib.org/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - diff --git a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell1_consensus_idXML_ylab_Counts_.svg b/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell1_consensus_idXML_ylab_Counts_.svg deleted file mode 100644 index 9944fe9..0000000 --- a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell1_consensus_idXML_ylab_Counts_.svg +++ /dev/null @@ -1,924 +0,0 @@ - - - - - - - - - 2023-09-15T08:24:35.228570 - image/svg+xml - - - Matplotlib v3.3.4, https://matplotlib.org/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - diff --git a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell2_consensus_idXML_ylab_Counts_.svg b/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell2_consensus_idXML_ylab_Counts_.svg deleted file mode 100644 index 965ef03..0000000 --- a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell2_consensus_idXML_ylab_Counts_.svg +++ /dev/null @@ -1,924 +0,0 @@ - - - - - - - - - 2023-09-15T08:24:35.605253 - image/svg+xml - - - Matplotlib v3.3.4, https://matplotlib.org/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - diff --git a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell3_consensus_idXML_ylab_Counts_.svg b/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell3_consensus_idXML_ylab_Counts_.svg deleted file mode 100644 index e9ef32f..0000000 --- a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell3_consensus_idXML_ylab_Counts_.svg +++ /dev/null @@ -1,924 +0,0 @@ - - - - - - - - - 2023-09-15T08:24:35.966552 - image/svg+xml - - - Matplotlib v3.3.4, https://matplotlib.org/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - diff --git a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell4_consensus_idXML_ylab_Counts_.svg b/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell4_consensus_idXML_ylab_Counts_.svg deleted file mode 100644 index dd1f2cd..0000000 --- a/multiqc_plots/svg/mqc_consensus_summary__name_Singlecell4_consensus_idXML_ylab_Counts_.svg +++ /dev/null @@ -1,924 +0,0 @@ - - - - - - - - - 2023-09-15T08:24:36.343560 - image/svg+xml - - - Matplotlib v3.3.4, https://matplotlib.org/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - diff --git a/multiqc_plots/svg/mqc_search_engine_PEP__name_100HeLacells_comet_feat_perc_idXML_ylab_Counts_.svg b/multiqc_plots/svg/mqc_search_engine_PEP__name_100HeLacells_comet_feat_perc_idXML_ylab_Counts_.svg deleted file mode 100644 index 7c106ab..0000000 --- a/multiqc_plots/svg/mqc_search_engine_PEP__name_100HeLacells_comet_feat_perc_idXML_ylab_Counts_.svg +++ /dev/null @@ -1,3116 +0,0 @@ - - - - - - - - - 2023-09-15T08:24:09.033702 - image/svg+xml - - - Matplotlib v3.3.4, https://matplotlib.org/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 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- - - - - - - - 2023-09-15T08:23:54.484255 - image/svg+xml - - - Matplotlib v3.3.4, https://matplotlib.org/ - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - diff --git a/pmultiqc/modules/quantms/quantms.py b/pmultiqc/modules/quantms/quantms.py index 572bffc..201237f 100755 --- a/pmultiqc/modules/quantms/quantms.py +++ b/pmultiqc/modules/quantms/quantms.py @@ -910,7 +910,7 @@ def CalHeatMapScore(self): pep_table.columns.tolist())) for name, group in pep_table.groupby("stand_spectra_ref"): - self.heatmap_con_score[name] = 1 - np.sum(np.sum( + self.heatmap_con_score[name] = 1.0 - np.sum(np.sum( group[group['accession'].str.contains(config.kwargs["contaminant_affix"])][study_variables])) / \ np.sum(np.sum(group[study_variables])) if config.kwargs['remove_decoy']: @@ -945,9 +945,9 @@ def CalHeatMapScore(self): worst = ((1 - y) ** 0.5) * 1 / n + (y ** 0.5) * (n - 1) / n sc = np.sum(np.abs(x - y) ** 0.5) / n if worst == 0: - self.ID_RT_score[name] = 1 + self.ID_RT_score[name] = 1.0 else: - self.ID_RT_score[name] = (worst - sc) / worst + self.ID_RT_score[name] = float((worst - sc) / worst) # For HeatMapOverSamplingScore self.HeatmapOverSamplingScore[name] = self.oversampling[name]['1'] / np.sum(list(self.oversampling[name].values())) @@ -1106,8 +1106,8 @@ def read_mzml_info(): charge_group = mzml_df.groupby("Charge").size() ms_level_group = mzml_df.groupby("MSLevel").size() charge_2 = charge_group[2] if 2 in charge_group else 0 - ms1_number = ms_level_group[1] if 1 in ms_level_group else 0 - ms2_number = ms_level_group[2] if 2 in ms_level_group else 0 + ms1_number = int(ms_level_group[1]) if 1 in ms_level_group else 0 + ms2_number = int(ms_level_group[2]) if 2 in ms_level_group else 0 self.total_ms2_spectra = self.total_ms2_spectra + ms2_number mzml_table[m].update({'MS1_Num': mzml_table[m]['MS1_Num'] + ms1_number}) mzml_table[m].update({'MS2_Num': mzml_table[m]['MS2_Num'] + ms2_number}) diff --git a/setup.py b/setup.py index 03bb4d2..7d5f2c7 100644 --- a/setup.py +++ b/setup.py @@ -7,11 +7,11 @@ from setuptools import setup, find_packages -version = '0.0.19' +version = '0.0.20' def readme(): - with open('README.md') as f: + with open('README.md', encoding="utf-8") as f: return f.read()

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