How to do 'Missing pixels are inferred from nearby data to facilitate clustering analysis across the entire mapped area'?

[xieduo7]

Hi Dr. @Zhiliang-Bai ,

How did you impute the expression matrix?
In the methods of the paper, you wrote:

But I didn't find corresponding code in this repository.

Thank you,

Best,
Duo

[Zhiliang-Bai]

Hi Duo,

We performed a straightforward imputation on our data to address an issue encountered during the experiment. Specifically, the horizontal channel 4 of our microfluidic device became clogged, which we unfortunately did not detect until after data collection was completed for all the samples. To mitigate this, we replaced the values for channel 4 with the average expression levels from channels 3 and 5.

I hope you find it helpful!

Best,
Zhiliang