sc-multiomics

During secondary palate development assembly, interactions with the adjacent epithelial and mesenchymal cells control the epithelial-mesenchymal communication for cell growth and differentiation during the craniofacial development.

Summary

The communication between neighboring tissues plays a central role in guiding organ morphogenesis. During secondary palate development assembly, interactions with the adjacent epithelial and mesenchymal cells control the epithelial-mesenchymal communication for cell growth and differentiation during the craniofacial development. However, the molecular underpinnings of this epithelial-mesenchymal relationship remain unclear. In this investigation, I conducted single-cell multiome sequencing analysis, simultaneously profiling chromatin accessibility and gene expression within the same individual cells (n = 35,160). These cells were isolated from the secondary palate of mice at varying embryonic days-E12.5, E13.5, E14.0, and E14.5. Here, the data show an essential role of cellular communication in directing the secondary palate formation at different stages of palatogenesis. The data analysis demonstrated that three signaling pathways including WNT, BMP, and PDGF suggested to steer the prominent developmental process of mice secondary palate during palatogenesis at E12.5 and then gradually diminished in their communication probabilities at E14.5. As a result, the nasal epithelium exhibits a higher level of enrichment in WNT signaling interaction with neighboring cells, while the oral epithelium displays greater enrichment in BMP signaling interaction. Additionally, the dental epithelium demonstrates increased enrichment in PDGF signaling communication. Together, these data uncover novel discoveries through which epithelial-mesenchymal communication can guide the cell movements that initiate secondary palate morphogenesis.

Figure 1.

Single-cell multiome assays were employed to analyze changes in both transcriptome and epigenome during the developmental stages of the mouse secondary palate.

Figure 2.

Integrative analysis of subpopulations in CNC-derived mesenchymal and epithelial cells.

Figure 3.

Unsupervised clustering of the major sources and targets from the interaction strength in two-dimensional manifold.

Figure 4.

CellChat analysis of the WNT signaling communication network in epithelial cells across different developmental stages.

Figure 5.

Analysis of BMP signaling network in epithelial cells at various developmental stages using CellChat.

Figure 6.

Analysis of PDGF signaling network in epithelial cells across diverse developmental stages using CellChat.