Are BLASTx annotations interchangeable?

[aspencoyle]

I'm prepping my new hemat_transcriptomev1.6 analysis for GO-MWU, and part of it involves cross-referencing with a BLASTx annotation table to obtain accession IDs.

When analyzing cbai_transcriptomev2.0 (which has both hematodinium and Chionoecetes sequences), I used this table, available from Genomic Resources

I believe (though am not 100% sure) that hemat_transcriptomev1.6 is derived from cbai_transcriptomev2.0. Is that the case? And if so, can I use the previously-used table to cross-reference sequence IDs and accession IDs?

[sr320]

Might it be best to generate a new blastx annotation table...

…

On Wed, Mar 10, 2021 at 3:35 PM afcoyle ***@***.***> wrote: I'm prepping my new hemat_transcriptomev1.6 analysis for GO-MWU, and part of it involves cross-referencing with a BLASTx annotation table to obtain accession IDs. When analyzing cbai_transcriptomev2.0 (which has both hematodinium and Chionoecetes sequences), I used this table <https://gannet.fish.washington.edu/Atumefaciens/20200608_cbai_diamond_blastx_v2.1_v3.1/cbai_transcriptome_v2.1.blastx.outfmt6>, available from Genomic Resources <https://robertslab.github.io/resources/Genomic-Resources/> I believe (though am not 100% sure) that hemat_transcriptomev1.6 is derived from cbai_transcriptomev2.0. Is that the case? And if so, can I use the previously-used table to cross-reference sequence IDs and accession IDs? — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub <#1145>, or unsubscribe <https://github.com/notifications/unsubscribe-auth/ABB4PNZN4S7BHWKEJNUAGFLTC7XWPANCNFSM4Y7DKZPQ> .

[aspencoyle]

Sounds good! Do I just BLAST my transcriptome against a database (say, the UniProt database) and then merge in R?

Roughly following this workflow here

[kubu4]

hemat_transcriptomev1.6 is derived from cbai_transcriptomev2.0. Is that the case?

No.

Deets can be found on Genomic Resources docs.

[sr320]

@afcoyle Yes - blast to Swiss-Prot/UniProt