USDA-SRGARP-gigas-carryover

Title: Improved climate resilience in oysters through optimization of hatchery-based environmental conditioning practices
2023-2024 USDA-NIFA Special Research Grants for Aquaculture Research Program (SRGARP)

Start: 2022-09-01
End: 2024-09-01

Pertinent documents

1. proposal
2. immune priming pilot shell measurements
3. Primers
4. manuscript
5. tissue google sheet
6. RNA extraction protocol
7. Initial qPCR results

Progress

1. 20221108 - received 100 Pacific oyster broodstock from USDA-ARS POGS; kept at ambient temperature (~12-14C)
2. 20230301 - oysters seperated into treatments. heat (13->30, 1hr/day) + mechanical stress (10min/3days) pre-treatments; temperature in tanks at 14C
3. 20230315 - oyster pre-treatments ended; third group of broodstock injected with poly(I:C); start of conditioning; temperature set at 14C (3d)
4. 20230318 - temperature increased to 15C (3d)
5. 20230321 - temperature increased to 16C (2d)
6. 20230323 - temperature increased to 17C (2d)
7. 20230325 - temperature increased to 18C (2d)
8. 20230327 - temperature increased to 19C (2d)
9. 20230329 - temperature increased to 20C (2d)
10. 20230331 - temperature increased to 21C (hold)
11. 20230428 - sample gonad for ripeness (4 weeks at 21C)
12. 20230727 - intitial qPCR results (injected vs control)

USDA: 62-7174

Winter-Spring 2024: Repeat stress conditiong experiment in 4 lifestages