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pDecaf_Coffee.gb
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LOCUS Exported 9466 bp DNA circular SYN 25-DEC-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pDecaf_Coffee
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9466)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 29, 2021 from SnapGene 5.3.2
https://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9466
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1219..1848
/label=pVS1 StaA
/note="pVS1 StaA"
CDS 2277..3350
/label=pVS1 RepA
/note="pVS1 RepA"
rep_origin 3416..3610
/label=pVS1 oriV
/note="pVS1 oriV"
misc_feature 3954..4094
/label=bom
/note="bom"
rep_origin complement(4280..4868)
/direction=LEFT
/label=ori
/note="ori"
CDS complement(4955..5749)
/label=KanR
/note="KanR"
misc_feature 6174..6198
/label=LB T-DNA repeat
/note="LB T-DNA repeat"
terminator 6276..6528
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(6585..7382)
/label=NeoR/KanR
/note="NeoR/KanR"
promoter complement(7445..8122)
/label=CaMV 35S promoter (enhanced)
/note="CaMV 35S promoter (enhanced)"
promoter 8349..8354
/label=lac promoter
/note="lac promoter"
promoter 8355..8372
/label=lac promoter
/note="lac promoter"
promoter 8373..8379
/label=lac promoter
/note="lac promoter"
protein_bind 8387..8403
/label=lac operator
/note="lac operator"
primer_bind 8411..8427
/label=M13 rev
/note="M13 rev"
promoter 8459..8803
/label=CaMV 35S promoter
/note="CaMV 35S promoter"
misc_feature 8804..8824
/note="siRNA Sense Strand"
misc_feature 8825..8833
/note="Nucleotide Spacer"
misc_feature 8834..8854
/note="siRNA Antisense strand"
terminator 8855..9107
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
CDS 9108..9301
/label=lacZ-alpha
/note="lacZ-alpha"
primer_bind complement(9142..9158)
/label=M13 fwd
/note="M13 fwd"
misc_feature 9361..9385
/label=RB T-DNA repeat
/note="RB T-DNA repeat"
ORIGIN
1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
4921 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
4981 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
5041 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
5101 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
5161 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
5221 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
5281 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
5341 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
5401 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
5461 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
5521 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
5581 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
5641 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
5701 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
5761 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
5821 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
5881 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
5941 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
6001 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
6061 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
6121 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
6181 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
6241 ttaatgtact gaattaacgc cgaattaatt cgggggatcg ttcaaacatt tggcaataaa
6301 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
6361 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
6421 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
6481 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcct cgagcttgtc
6541 gatcgactct agctagagga tcgatccgaa ccccagagtc ccgctcagaa gaactcgtca
6601 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
6661 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
6721 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
6781 ttttccacca tgatattcgg caagcaggca tcgccatgtg tcacgacgag atcctcgccg
6841 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
6901 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
6961 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
7021 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
7081 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
7141 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgg
7201 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
7261 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
7321 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcccc
7381 atggtcgatc gacagatctg cgaaagctcg agagagatag atttgtagag agagactggt
7441 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga
7501 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
7561 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
7621 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
7681 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
7741 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
7801 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
7861 caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
7921 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
7981 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
8041 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
8101 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
8161 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
8221 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
8281 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
8341 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
8401 caatttcaca caggaaacag ctatgaccat gattacgaat tcgagctcgg tacccgggtg
8461 agacttttca acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct
8521 gtcacttcat caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg
8581 ataaaggaaa ggctatcgtt caagatgccc ctgccgacag tggtcccaaa gatggacccc
8641 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
8701 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
8761 acccttcctc tatataagga agttcatttc atttggagag gaccagattg taggatgagt
8821 tcttttcaag agagtctaac atcctactca agaagatcgt tcaaacattt ggcaataaag
8881 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
8941 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
9001 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
9061 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcctc tagagtcgac
9121 ctgcaggcat gcaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
9181 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
9241 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta
9301 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
9361 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata
9421 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtg
//