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Welcome to the Tumor-Digital-BioDynamics wiki!

The first project in this repository is the Tumor Cell Number code which estimates the number of Tumor Cells in a lesion of a given size. It estimates this by evaluating the volume of the lesion the average volume of the tumor cells.

It then estimates the amount of tumor and non-tumor DNA in the lesion, after compensating for percent stroma (non-tumor), and the cellularity of the stroma.

Finally it gives an estimate of the number of 10um tissue sections, from formalin fixed paraffin embedded tumor, that would be necessary to assure at least 100 ng of Tumor DNA, required for most long read sequencing methods.

This is a Python script written in Spyder (WINPY 64 port), and uses PySimpleGUI to create a form and data calculation page.

PyInstaller was used to create the executable (which requires the DNA logo image in the same folder.

The installer was created with Inno Setup.