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Error with running SPAdes: The program was terminated by segmentation fault #231

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SowmyaPulapet opened this issue Jan 23, 2023 · 4 comments

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@SowmyaPulapet
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Hi,
I using GetOrganelle for the first time. I followed the method using setup.py to install GetOganelle. The dependencies are from GetOrganelleDep. My data has approximate size of ~9 gb. The command I am using is :

/Tools/GetOrganelle/get_organelle_from_reads.py -1 data_1.fq.gz -2 data_2.fq.gz -o data_output -R 15 -F embplant_pt -t 10

I am getting the following error:

ERROR: Error with running SPAdes: The program was terminated by segmentation fault
ERROR: Assembling failed.

The log file has been attached here. Hope someone would help me.

Thank you.
log.txt

@JianjunJin
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It should be similar to many of the previous issues, e.g. #226

So, what is the outcome of spades.py --test coming from the SPAdes of GetOrganelleDep?

@SowmyaPulapet
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I got this error while running spades.py --test :

== Error == system call for: "['/Tools/GetOrganelle/GetOrganelleDep/linux/SPAdes/bin/spades-hammer', '/Tools/GetOrganelle/GetOrganelleDep/linux/SPAdes/bin/spades_test/corrected/configs/config.info']" finished abnormally, OS return value: -11

Here's the complete log spades.log

@JianjunJin
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It seems the version at GetOrganelleDep is not working in your environment.
Is it possible for you to install SPAdes from other approaches, like from the SPAdes website or GitHub?
After you install SPAdes by your own and add spades bin to the $PATH, you should remove the entire directory of /Tools/GetOrganelle/GetOrganelleDep/linux/SPAdes/, thus GetOrganelle can automatically detect the new SPAdes.

Please let me know you updates.

@SowmyaPulapet
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I removed /Tools/GetOrganelle/GetOrganelleDep/linux/SPAdes/ as you said. I didn't have to install spades again, had previusly installed one. Now the assembly is finished.

Thank you ! @JianjunJin

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