Skip to content

14zac2/DiseasedWoodchuck

Repository files navigation

WHV Analysis

Analysis of woodchucks infected with the Woodchuck Hepatitis Virus (WHV)

File list

run_arriba_starsolo_scRNAseq_piped.sh - Run STARSolo and Arriba on scRNA-seq FASTQ files to find integration sites. Makes two directories: starSolo_whitelist_stringent_output and arriba_output_solo_stringent which contain STAR and Arriba outputs respectively.

intSite_cellAssociation_scRNAseq.sh - Associate specific clusters with integration sites found with Arriba. Uses input from makeBarcodeFusionMatrix.sh and a TSV file of cell barcodes and their associated cluster from processed scRNA-seq data (cluster_barcode_IDs.tsv) generated in R.

findIntegrationPatterns.sh - Pulling out important information from Arriba output fusions.tsv to compare across samples.

makeBarcodeFusionMatrix.sh - Make a matrix of the number of fusion events for each cell based on Arriba output. Requires Arriba output fusions.tsv and R1 barcodes FASTQ files from CellRanger (/file/path/to/fastqs/*R1*).

About

Analysis of woodchucks infected with WHV.

Resources

Stars

Watchers

Forks

Releases

No releases published

Packages

No packages published

Languages